Consulta: subjectFacets:"Bioinformatics analysis"
Registros recuperados: 5
Data/hora: 14/06/2024 03:09:01

Comparative Analysis of C-repeat Binding Factors (CBFs) in Tomato and Arabidopsis
Provedor de dados: 52
Autores: Heidari,Parviz.
Abstract Cold stress is one of the limiting factors of plant production that plants use different mechanisms for cold tolerance. CBF genes play critical role to regulate the cold responsive genes. To better understand of CBF gene functions, the tomato-CBFs and Arabidopsis-CBFs were evaluated using bioinformatics tools, and finally the expression patterns of SlCBF1 gene were analyzed under 10 and 4˚C in two contrasting tomato species (Solanum lycopersicum and S. habrochaites). The different cis regulatory elements were observed in promoter region of SlCBF1 and AtCBF1 genes, and ICE1, COR and HOS1 proteins exhibited high interaction with CBFs. The results of Real time PCR of SlCBF1 exhibited that under 10 and 4 ˚C, SlCBF1 was down regulated in cold sensitive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cold stress; CBF genes; Real time PCR; Tomato; Bioinformatics analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100208

Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments
Provedor de dados: 58
Autores: Ser,Hooi-Leng; Tan,Wen-Si; Ab Mutalib,Nurul-Syakima; Yin,Wai-Fong; Chan,Kok-Gan; Goh,Bey-Hing; Lee,Learn-Han.
ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome sequence; Streptomyces; Mangrove; AntiSMASH; Bioinformatics analysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100013

Comparative analysis of two component signal transduction systems of the Lactobacillus acidophilus group
Provedor de dados: 58
Autores: Cui,Yanhua; Qu,Xiaojun.
The Lactobacillus acidophilus group is a phylogenetically distinct group of closely related lactobacilli. Members of this group are considered to have probiotic properties and occupy different environmental niches. Bacteria generally sense and respond to environmental changes through two component systems (TCSs) which consist of a histidine protein kinase (HPK) and its cognate response regulator (RR). With the use of in silico techniques, the five completely sequenced L. acidophilus group genomes were scanned in order to predict TCSs. Five to nine putative TCSs encoding genes were detected in individual genomes of the L. acidophilus group. The L. acidophilus group HPKs and RRs were classified into subfamilies using the Grebe and Stock classification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus acidophilus group; Two component system; Histidine protein kinase; Response regulator protein; Bioinformatics analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100019

Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
Provedor de dados: 69
Autores: Zhong,Tianxiu; Zhu,Chen; Zeng,Huiming; Han,Liebao.
Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproduction. Leaves from plants subjected to drought stress provided the source of &amp;#8216;Tester&amp;#8217; DNA, and leaves from plants grown under normal conditions provided the &amp;#8216;Driver&amp;#8217; DNA for subtractive hybridization. Results: A total of 481 high quality ESTs were generated, which clustered into 390 unigenes. Of these unigenes, 132 grouped into 12...
Tipo: Journal article
Palavras-chave: Bioinformatics analysis; Real time PCR; Suppression subtractive hybridization.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004

A functional polymorphism in the paired basic amino acid-cleaving enzyme 4 gene confers osteoarthritis risk in a population of Eastern China
Provedor de dados: 74
Autores: He,Jin; Yang,Haoyu; Xu,Zhonghua; Li,Jin; Chen,Gang; Jiang,Lifeng; Wu,Lidong; Zhou,Xindie.
Abstract Paired basic amino acid-cleaving enzyme 4 (PACE4), a proprotein convertase, is involved in the activation of aggrecanases (ADAMTS-4 and ADAMTS-5) in osteoarthritic and cytokine-stimulated cartilage. Activated aggrecanases cause aggrecan degradation and thus, contribute to osteoarthritis (OA). In this study, we investigated the association between PACE4 gene polymorphisms and OA risk. One single-nucleotide polymorphism (rs4965833) in the PACE4 gene was genotyped in 432 OA patients and 523 healthy controls using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Quantitative reverse transcription PCR (qRT-PCR) was used to determine the relative expression of PACE4 in blood samples from 90 OA patients (30 for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ACE4; Osteoarthritis; Bioinformatics analysis; Single-nucleotide polymorphism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100104