Consulta: subjectFacets:"BoHV-5"
Registros recuperados: 8
Data/hora: 06/06/2024 01:21:47

Co-infections with bovine herpesvirus type 5 and bovine viral diarrhoea virus
Provedor de dados: 45
Autores: Spilki,F.R.; Silva,T.C.; Esteves,P.A.; Teixeira,M.B.; Batista,H.B.C.R.; Chiminazzo,C.; Driemeier,D.; Franco,A.C.; Roehe,P.M.
During a series of experiments attempting to reproduce clinically apparent bovine herpesvirus type 5 (BoHV-5) infections, a group of calves was inadvertently infected with bovine viral diarrhoea virus (BVDV). Another group of calves was infected with BoHV-5 only. This paper reports the outcome of such infections. Two out of six calves solely infected with BoHV-5 displayed moderate to severe clinical signs. Three out of four calves of the group co-infected with BoHV-5 and BVDV developed severe clinical signs, two of them died. BoHV-5 virus was isolated to higher titres and for a longer period from the group of calves infected with both viruses. These results suggest that BVDV may enhance clinical signs induced by BoHV-5 and may also play a role in extending...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; BVDV; Co-infection; Experimental inoculation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500001

Utilização de parte da região codificadora da glicoproteína b na diferenciação do herpesvírus bovino 1.1, herpesvírus bovino 1.2 e herpesvírus bovino 5
Provedor de dados: 45
Autores: Costa,E.A.; Barbosa-Stancioli,E.F.; Leite,R.C.; Oliveira,G.D.R.; Rocha,M.A.
Utilizou-se uma reação em cadeia de polimerase (PCR) previamente desenvolvida para a amplificação de parte da região única longa 27 (UL27) do genoma de herpesvírus bovino 1.1 (BoHV-1), que codifica a glicoproteína B, buscando a diferenciação entre isolados de BoHV-1.1 e BoHV-5. Os produtos de PCR gerados a partir de isolados de BoHV-1.1 e BoHV-5 mostraram padrão de amplificação diferenciado em seus tamanhos moleculares. Analisando as seqüências de nucleotídeos dos produtos de PCR obtidos de dois isolados de BoHV-5, juntamente com as seqüências dos produtos de PCR obtidos de um isolado de BoHV-1.1 e de três isolados de BoHV-1.2, previamente depositados no GenBank, verificou-se que a diferença observada na amplificação se deve ao número de repetições de G-C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-1.1; BoHV-1.2; BoHV-5; GB; PCR; Seqüências-motivo.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000200001

The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
Provedor de dados: 52
Autores: Lima,Francisco Esmaile de Sales; Batista,Helena Beatriz Ruthner; Campos,Fabrício Souza; Kunert Filho,Hiran Castagnino; Cibulski,Samuel; Finoketti,Fernando; Arantes,Thalita; Spilki,Fernando; Roehe,Paulo Michel; Rijsewijk,Franciscus Antonius Maria; Franco,Ana Cláudia.
This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; V mRNA; Transfection.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007

A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits
Provedor de dados: 56
Autores: Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I.
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005

Selection and characterization of brivudin resistant bovine herpesvirus type 5
Provedor de dados: 58
Autores: Brum,Mário Celso Sperotto; Santos,Cyndia Mara Bezerra dos; Weiblen,Rudi; Flores,Eduardo Furtado.
Bovine herpesvirus type 5 (BoHV-5) is the agent of meningoencephalitis, an important disease of cattle in South America. The neuropathogenesis of BoHV-5 infection is poorly understood and most previous research focused on the role of envelope glicoproteins in neurovirulence. Thymidine kinase (TK) is a viral enzyme necessary for virus replication in neurons and, therefore, represents a potential target for virus attenuation. The selection and characterization of BoHV-5 variants resistant to the nucleoside analog brivudin (BVDU), which selects TK-defective viruses is here described. Several BVDU-resistant clones were obtained after multiple passages in tissue culture in the presence of BVDU and one clone (BoHV-5/R-27) was further characterized. The selected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-5; Thymidine kinase; Drug resistant; Brivudin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100019

Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the gI, gE and US9 genes
Provedor de dados: 58
Autores: Franco,Ana Cláudia; Hübner,Sílvia de Oliveira; Oliveira,Anna Paula de; Batista,Helena B. de C. Ruthner; Roehe,Paulo Michel; Rijsewijk,Franciscus A.M.
Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus 5; BoHV-5; Deletion mutant; Glycoprotein genes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400016

Produção e caracterização de anticorpos monoclonais contra uma cepa do herpesvírus bovino tipo 1 defectiva na glicoproteína C (gC)
Provedor de dados: 65
Autores: Winkelmann,Evandro Reinoldo; Silva,Letícia Frizzo da; Mayer,Sandra Vanderli; Mazzutti,Ketty Cristina; Weiblen,Rudi; Flores,Eduardo Furtado.
A maioria dos anticorpos monoclonais (AcMs) já produzidos contra o herpesvírus bovino tipo 1 (BoHV-1) reage com a glicoproteína C (gC), um antígeno abundante e imunodominante presente no envelope viral. Com o objetivo de produzir AcMs com outras especificidades protéicas, antígenos de uma cepa do BoHV-1 defectiva no gene da gC foram utilizados para a imunização de camundongos BALB/c. Após fusão e seleção de 54 hibridomas resistentes ao meio seletivo HAT, foram obtidos três clones (1F1, 2H4 e 4D7) secretores de imunoglobulinas da classe IgG2a, que reagiram com antígenos da cepa homóloga. Os AcMs reagiram com antígenos virais nas técnicas de imunofluorescência (IFA) e imunoperoxidase (IPX) em diluições de até 1:640 (sobrenadante de cultivo) e 1:20.000...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herpesvírus bovino; BoHV-1; BoHV-5; Anticorpos monoclonais; Neutralização.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400024

Imunogenicidade de vacinas comerciais inativadas contra o herpesvírus bovino tipo 1
Provedor de dados: 65
Autores: Silva,Letícia Frizzo da; Weiblen,Rudi; Flores,Eduardo Furtado.
O presente trabalho avaliou a imunogenicidade de seis vacinas comerciais contendo antígenos inativados do herpesvírus bovino tipo 1 (BoHV-1): uma brasileira (BR), uma norte-americana (US), duas uruguaias (UR1 e UR2) e duas argentinas (ARG1 e ARG2). Para isso, grupos de bovinos foram vacinados com duas doses (dias 0 e 21) de cada uma das vacinas. Anticorpos neutralizantes contra o BoHV-1 e o BoHV-5 foram pesquisados no soro colhido 21 dias após a segunda dose. Com exceção das vacinas US e UR1, as demais vacinas induziram títulos baixos de anticorpos na maioria dos animais. Os títulos induzidos pela vacina US (média geométrica, GMT=38) foram superiores aos demais (P&lt;0,05). A vacina UR1 induziu títulos (GMT=20,2) superiores aos induzidos pelas vacinas BR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BoHV-1; BoHV-5; Vacinas comerciais; Brasil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000500042