Consulta: subjectFacets:"Bone regeneration"
Registros recuperados: 16
Data/hora: 08/06/2024 11:57:31

Electrical Stimulation in the Bone Repair of Defects Created in Rabbit Skulls
Provedor de dados: 77
Autores: Silva,C; Olate,S; Pozzer,L; Muñoz,M; Cantín,M; Uribe,F; de Albergaría-Barbosa,J. R.
Electrical stimulation has been used in different conditions for tissue regeneration. The aim of this study was to analyze the tissue response of defects created in rabbit skulls to electrical stimulation. Two groups were formed, each with 9 New Zealand rabbits; two 5 mm defects were made, one in each parietal, with one being randomly filled with autogenous bone extracted as particles and the other maintained only with blood clotting. The rabbits were euthanized at 8 weeks and 15 weeks to then study the samples collected histologically. In the 8-week analysis bone formation was observed in the defects in the test and control filled with bone graft, whereas the defects with clotting presented a very early stage of bone formation with abundant connective...
Tipo: Journal article
Palavras-chave: Electrical stimulus; Bone regeneration; Bone graft.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300054

Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets
Provedor de dados: 77
Autores: Fuentes,Ramón; Oporto Venegas,Gonzalo; Olate,Sergio; Rojas Rauco,Mariana; Borie,Eduardo.
Within oral rehabilitation alveolar ridge preservation following extraction is important. This research study shows a histological, histochemical and histomorphometrical evaluation in two cases of post extraction ridge-socket preservation performed with FDBA. In two patients dental extraction procedures were performed and sockets were immediately filled with FDBA. Six months later a biopsy of grafted area was obtained and rehabilitated through dental implant. Grafted bone samples were treated for histological and histochemical analysis. Bone tissue area was measured. Laboratory analysis of three samples showed inactive bone surfaces, neither osteoblasts nor osteoclasts were found, only osteocyte and osteogenous cells were observed. These findings do not...
Tipo: Journal article
Palavras-chave: Bone regeneration; Biomaterials; FDBA; Socket.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100047

Evaluation of Decellularized Matrix and ß-Tricalcium Phosphate as Biomaterials for Bone Neoformation: In vivo Study
Provedor de dados: 77
Autores: Muñoz-Ruíz,Abraham; Silva-Benítez,Erika; Soto-Sainz,Eduardo; Cerda-Cristerna,Bernardino; Ortiz-Magdaleno,Mariné; Pozos-Guillen,Amaury; Flores,Héctor.
The aim of this study was to evaluate histologically the effect of two biomaterials, a biomaterial derived from porcine Urinary submucosa Bladder Matrix (UBM) and beta-TriCalcium Phosphate (ß-TCP), on bone defects. Twenty male New Zealand rabbits were used; the models were divided in two groups: the UBM group; the ß-TCP group, and a Negative Control (NC) group. Five-mm defects were created in the femur of each model and then the different biomaterials were set in place depending on each group. At 4 and 8 weeks, the animals in the models were sacrificed and samples of the defect site were collected to perform a Hematoxylin and Eosin stain (H&amp;E). Histologically, ß-TCP group at 4 and 8 weeks presented neoformation of bone-like and cartilage-like tissue,...
Tipo: Journal article
Palavras-chave: Urinary bladder matrix; Bone regeneration; Biomaterial; SS-tricalcium phosphate.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300003

Bone Healing in Transverse Maxillary Defects with Different Surgical Procedures Using Anorganic Bovine Bone in Humans
Provedor de dados: 77
Autores: Beltrán,Victor; Matthijs,Andries; Borie,Eduardo; Fuentes,Ramón; Valdivia-Gandur,Iván; Engelke,Wilfried.
The centripetal resorption of maxilla is a continuous process after tooth loss. For treatment of deficient bone sites, autologous bone grafts may be used, as an alternative, biomaterials can be applied which do not require intra- or extraoral donor sites. The present report we describe the use of anorganic bovine bone (ABB) based on three case reports under different modes: Membrane, rigid barrier and connective tissue graft. Clinical results show that under all conditions, sufficient hard tissue was formed to allow implant born rehabilitation in the former insufficient bone sites. Histologic evaluation revealed trabecular bone in an almost mature matrix with resting particles of ABB. Depending on the original site situation, two step rigid occlusive...
Tipo: Journal article
Palavras-chave: Bone regeneration; Maxilla; Dental implant; Bone defect; Biomaterials.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100011

The Behavior of Demineralized Bone Matrix (DBM) in Post-Extraction Sockets
Provedor de dados: 77
Autores: Fuentes,Ramón; Issa,João Paulo Mardegan; Iyomasa,Mamie Mizusaki; Oporto,Gonzalo; Prieto,Ruth; Borie,Eduardo.
Autogenous bone grafts are considered to be the gold standard in bone regeneration because of their osteogenic activity; however, due to limited availability of intraoral donor sites and the need to resolve the demands of patients requires an alternative to these. Two male patients were submitted to implant surgery in two stages with 6 months intervals between each of them: the first was exodontia and placement of DBM graft into the socket; the second stage was the drill with a 2 mm internal diameter trephine in center of the alveolar ridge previously grafted with DBM and subsequent implant placement. The samples were analyzed under histological techniques. A very mature bone was observed at 6 months after DBM graft placement in the sockets, showing it to...
Tipo: Journal article
Palavras-chave: Bone regeneration; Biomaterials; DBM; Socket.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200005

Bone Augmentation With Occlusive Barriers and Cortical Particulate Allograft in Transverse Maxillary Defects: A Pilot Study
Provedor de dados: 77
Autores: Beltrán,Víctor; Engelke,Wilfried; Fuentes,Ramón; Decco,Oscar; Prieto,Ruth; Wilckens,Mario; Borie,Eduardo.
The centripetal resorption of maxilla is a continuous process after tooth loss. For treatment of deficient bone sites, autologous bone grafts may be used, as an alternative, biomaterials can be applied which do not require intra or extraoral donor sites. The present report describes the use of occlusive barriers and cortical particulate allograft in transverse maxillary defects. This surgical approach was performed in five patients (4 females and 1 male, aged 20 to 37 years). Clinical results show that sufficient hard tissue was formed to allow implant born rehabilitation in the former insufficient bone sites. Histological evaluation revealed small amounts of newly formed bone with a predominance of collagen fibrous tissue and mature bone with very little...
Tipo: Journal article
Palavras-chave: Bone regeneration; Maxilla; Dental implant; Bone defect; Biomaterials.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100057

Fibrin biopolymer as scaffold candidate to treat bone defects in rats
Provedor de dados: 80
Autores: Cassaro,Claudia Vilalva; Justulin Jr.,Luis Antonio; Lima,Patrícia Rodrigues de; Golim,Marjorie de Assis; Biscola,Natália Perussi; Castro,Mateus Vidigal de; Oliveira,Alexandre Leite Rodrigues de; Doiche,Danuta Pulz; Pereira,Elenize Jamas; Ferreira Jr.,Rui Seabra; Barraviera,Benedito.
ABSTRACT  Background:  Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs.  Methods:  Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Biomaterials; Fibrin sealant; Fibrin biopolymer; Biphasic calcium phosphate; Mesenchymal stem cells.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320

Synthesis of calcium-phosphate and chitosan bioceramics for bone regeneration
Provedor de dados: 42
Autores: FINISIE,MELLATIE R.; JOSUÉ,ATCHE; FÁVERE,VALFREDO T.; LARANJEIRA,MAURO C. M.
Bioceramic composites were obtained from chitosan and hydroxyapatite pastes synthesized at physiological temperature according to two different syntheses approaches. Usual analytical techniques (X-ray diffraction analysis, Fourier transformed infrared spectroscopy, Thermo gravimetric analysis, Scanning electron microscopy, X-ray dispersive energy analysis and Porosimetry) were employed to characterize the resulting material. The aim of this investigation was to study the bioceramic properties of the pastes with non-decaying behavior from chitosan-hydroxyapatite composites. Chitosan, which also forms a water-insoluble gel in the presence of calcium ions, and has been reported to have pharmacologically beneficial effects on osteoconductivity, was added to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioceramic; Chitosan; Hydroxyapatite; Composites; Bone regeneration.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000400006

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism
Provedor de dados: 45
Autores: Buchaim,R.L.; Andreo,J.C.; Rodrigues,A. C.; Buchaim,D.V.; Dias,D.V.; Daré,L.R.; Roque,D.D.; Roque,J.S.
The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat; Bone regeneration; Alcoholism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300016

Bone regeneration in mandible defect with autograft bone and cell suspension from bone marrow in rabbits
Provedor de dados: 45
Autores: Gomes,C.; Paz,A.H.; Bonilla,A.T.; Corrêa,A.; Cavalcante,R.L.; Veiga,D.; Colomé,L.M.; Foerstnow,L.P.; Contesini,E.A.; Cirne-Lima,E.O.
The objective of this study was to investigate the bone regeneration of a "gold standard" (autograft) from iliac crest associated with cellular therapy in rabbits. A bone defect was created with 10x5x5mm in 28 rabbit mandibles. The control group animals (n=14) were repaired with autograft of iliac crest and the experimental group animals (n=14) received iliac crest autograft in association with mononuclear cells from the bone marrow of the femur. Weekly radiographs were taken of the surgery region and histological analyses was performed in seven animals in each group at 15 days and in seven animals of each group at 30 days after the surgery. A gradual increase of bone density was observed and the experimental animals presented the bone bridge in 85.7%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabbit; Mandibular reconstruction; Bone regeneration; Graft.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400007

Hydroxyapatite, polycaprolactone and alendronate composites for bone regeneration in rabbits' olecranon: histological features
Provedor de dados: 45
Autores: Valente,F.L.; Reis,E.C.C.; Sepúlveda,R.V.; Ochoa,C.C.R.; Santos,L.C.; Corsini,C.M.; Borges,A.P.B.
O presente trabalho teve como objetivo avaliar a biocompatibilidade e a osseointegração de compósitos de hidroxiapatita (HA), policaprolactona (PCL) e alendronato (ALN) em defeitos ósseos produzidos no olécrano de coelhos. Trinta e seis coelhos foram distribuídos em quatro grupos, recebendo como tratamento: (1) compósito de HA (49,5%), PCL (49,5%) e ALN (1%); (2) HA (50%) e PCL (50%); (3) PCL (100%); e (4) solução salina 0,9%. As amostras para análise histológica foram coletadas de três animais de cada grupo aos oito, 45 e 90 dias de pós-operatório. No oitavo dia, as bordas do defeito ainda eram identificáveis em todos os grupos. Tecido ósseo novo era formado em contato com o biomaterial apenas nas formulações que incluíam HA. Essas características...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bone regeneration; Biocompatibility; Biomaterial.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200543

Using mesenchymal stem cells as a therapy for bone regeneration and repairing
Provedor de dados: 48
Autores: Shao,Jin; Zhang,Weiwei; Yang,Tieyi.
Bone is a unique tissue which could regenerate completely after injury rather than heal itself with a scar. Compared with other tissues the difference is that, during bone repairing and regeneration, after the inflammatory phase the mesenchymal stem cells (MSCs) are recruited to the injury site and differentiate into either chondroblasts or osteoblasts precursors, leading to bone repairing and regeneration. Besides these two precursors, the MSCs can also differentiate into adipocyte precursors, skeletal muscle precursors and some other mesodermal cells. With this multiline-age potentiality, the MSCs are probably used to cure bone injury and other woundings in the near future. Here we will introduce the recent developments in understanding the mechanism of...
Tipo: Journal article
Palavras-chave: Mesenchymal stem cells; Bone regeneration; Bone repairing; Tissue engineering.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100062

Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair
Provedor de dados: 52
Autores: Maia Filho,Antonio Luiz Martins; Amaral,Fabrício Pires de Moura do; Martin,Airton Abrahão; Soares,Luís Eduardo Silva.
The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Alumina foams; Apatite coating; FT-Raman spectroscopy; Micronucleus test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004

Thymbra Spicata Var. Intricata Induces Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation
Provedor de dados: 52
Autores: Mendi,Ayseqül; Yağcı,Beyza Gökçınar; Kızıloğlu,Mustafa; Saraç,Nurdan; Uğur,Aysel; Yılmaz,Derviş; Uçkan,Duygu.
ABSTRACT A natural agent that maintains mesenchymal stem cell (MSCs) viability, promotes osteogenic differentiation while modulating the immunological response could achieve success in regeneration during healing and may also prevent bone resorption and improve regeneration. We aimed to demonstrate that a Thymbra spicata var. intricata extract could induce proliferation, differentiation, and modulate the immune responses of mesenchymal stem cells (MSCs). Using xCELLigence, a real-time monitoring system, we obtained a growth curve of MSCs. A dose of 10 µg/mL was the most efficient concentration for vitality. Osteogenic differentiation and antiinflammatory activities were determined by using an ELISA Kit to detect early and late markers of differentiation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cell; Osteogenic differentiation; T. spicata var. intricata; Bone regeneration.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100310

BONEFILL ® block as alternative for bone substitute: a toxicological evaluation
Provedor de dados: 60
Autores: Melchior,Karine; Saska,Sybele; Coelho,Fernanda; Scarel-Caminaga,Raquel Mantuaneli; Capote,Ticiana Sidorenko de Oliveira.
ABSTRACT Bone substitutes based on hydroxyapatite (HA) and Bonefill® (BO - inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BONEFILL®/toxicity; Biomaterials/evaluation; Bone regeneration; Hydroxyapatite.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200611

Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs
Provedor de dados: 65
Autores: Alves,Endrigo Gabellini Leonel; Serakides,Rogéria; Rosado,Isabel Rodrigues; Paez,Omar Leonardo Aristizabal; Varon,Jéssica Alejandra Castro; Machado,Felipe Nemer; Fukushima,Fabíola Bono; Góes,Alfredo Miranda; Rezende,Cleuza Maria de Faria.
ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Orthopedics; Cellular therapy; Fracture non-union.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700604