Consulta: subjectFacets:"Borrelia"
Registros recuperados: 2
Data/hora: 05/06/2024 17:34:07

Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
Provedor de dados: 45
Autores: Radulović,Ž.; Milutinović,M.; Tomanović,S.; Mulenga,A.
A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015

Evaluation of antibodies production against Borrelia burgdorferi in cattle submitted to rBm86 protein Boophilus microplus tick immunization and associated challenges influence
Provedor de dados: 58
Autores: Ishikawa,Márcia Mayumi; Fonseca,Adivaldo Henrique; Yoshinari,Natalino Hajime; Osório,Ana Luiza Alves Rosa.
IgG antibodies production against Borrelia burgdorferi in immunized cattle with rBm86 protein from Boophilus microplus was evaluated as well as the influence of the association between immunizations and stress through indirect ELISA test during one year. In the present study there was no influence of the isolated challenged used on the production of IgG antibodies against B. burgdorferi. The rBm86 immunogen did not cause significant oscillation in the production of IgG antibodies against B. burgdorferi capable to interfere in the serological results for Lyme Borreliosis in cattle. This study demonstrated the possibility of transitory changes in the production of antibodies after the association of vaccine stimuli and stress, emphasizing the necessity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibodies; Borrelia; Cattle; Tick.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500032