Consulta: subjectFacets:"CDNA sequences"
Registros recuperados: 2
Data/hora: 09/06/2024 10:26:54

Characterisation and expression of four mRNA sequences encoding glutathione S-transferases pi, mu, omega and sigma classes in the Pacific oyster Crassostrea gigas exposed to hydrocarbons and pesticides
Provedor de dados: 5
Autores: Boutet, Isabelle; Tanguy, Aurelie; Moraga, Dario.
Hydrocarbon and pesticide pollution in coastal ecosystems can disturb marine bivalve metabolism. In this study, we characterised four full-length cDNA sequences encoding glutathione S-transferases (GSTs) in the Pacific oyster Crassostrea gigas. A BLAST X search showed that these four sequences encode GSTs from four different classes: GST pi, sigma, mu and omega. A phylogenetic analysis of GST was made to determine the position of oyster GST compared to invertebrate and vertebrate sequences. We developed a semi-quantitative, multiplex RT-PCR to follow the expression of these four GSTs in tissues of oysters exposed to hydrocarbons and two pesticide treatments ( glyphosate and a mixture composed of atrazine, diuron and isoproturon) under experimental...
Tipo: Text
Palavras-chave: Crassostrea gigas; Gene expression; Multiplex RTPCR; Pesticides; Hydrocarbons; CDNA sequences; Glutathione S transferases.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-431.pdf

A simple RT-PCR-based strategy for screening connexin identity
Provedor de dados: 56
Autores: Urban,M.; Rozental,R.; Spray,D.C.
Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx) proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes after experimental manipulation has become quite laborious. Here we describe an efficient, rapid and simple method by which connexin type(s) can be identified in mammalian tissue and cultured cells using endonuclease cleavage of RT-PCR products generated from "multi primers" (sense primer, degenerate oligonucleotide corresponding to a region of the first extracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gap junctions; Intercellular communication; CDNA sequences; Universal primers; Multi primers.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800014