Consulta: subjectFacets:"COI"
Registros recuperados: 48
Data/hora: 14/06/2024 13:48:28

Variación morfométrica y genética de Diaphorina citri (Hemiptera: Liviidae) en rutáceas de Veracruz, México.
Provedor de dados: 32
Autores: Cuellar Sánchez, Valeria Isabel.
Diaphorina citri es el vector más importante del Huanglongbing, la enfermedad más destructiva de los cítricos en el mundo. El objetivo de este estudio fue detectar la presencia de variantes morfométricas y genéticas, a través del análisis de secuencias de nucleótidos mediante RAPDs y el gen mitocondrial mtCOI para determinar si existe correspondencia entre variantes de individuos de D. citri Kuwayama (Hemiptera: Liviidae), colectados en rutáceas de Veracruz, México. Se encontraron variaciones morfométricas en el ancho, largo y venación de las alas y de los procesos genales de los especímenes estudiados. Los análisis genéticos por medio de RAPDs y secuencias obtenidas con el gen mtCOI confirman la existencia de cuatro haplotipos (H1-H4) de D. citri...
Palavras-chave: Psílido asiático de los cítricos; Morfometría; RAPDs; COI; PCR; Asiatic citrus psyllid; Morphometrics; Entomología y Acarología; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2346

Estructura genética de poblaciones de Oligonychus perseae y Oligonychus punicae (Acari: Tetranychidae) en huertos de aguacate.
Provedor de dados: 32
Autores: Guzmán Valencia, Stephanie.
La identidad y estructura genética de poblaciones de Oligonychus punicae y O. perseae (Acari: Tetranychidae) fue estudiada en siete localidades productoras de aguacate Persea americana var. Hass. La identidad de los ácaros fue determinada morfológica y molecularmente mediante la secuenciación de una fracción del gen Citocromo Oxidasa subunidad I (COI). La variación genética intraespecífica de diferentes poblaciones de ambas especies de ácaros fue estudiada mediante la secuenciación de las regiones ITS1 e ITS2 del rADN.  El análisis filogenético de las secuencias parciales del gen COI separó adecuadamente las dos especies de ácaros, confirmando los resultados de la identificación morfológica. El análisis de las secuencias de las regiones ITS1 e ITS2 del...
Palavras-chave: COI; ITS1; ITS2; Estructura genética; Entomología y Acarología; Maestría; Genetic structure.
Ano: 2012
URL: http://hdl.handle.net/10521/1765

ANALISIS MOLECULAR EN GASTROPODOS CHILENOS COMERCIALES BASADOS EN LAS SECUENCIAS 16S rRNA,. COI Y ITS1-5.8S rDNA-ITS2
Provedor de dados: 72
Autores: Aguilera-Muñoz,Felipe; Lafarga-Cruz,Fabiola; Gallardo-Escárate,Cristian.
Gastropod mollusks are part of the principal marine resources cultivated and commercialized in Chile. There are native Chilean species such as loco (Concholepas concholepas), locate (Thais chocolata), trumulco snail (Chorus giganteus), keyhole limpets (Fissurella spp.), tegula snail (Tegula atra) as well as exotic species such as red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai). Despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. The aim of this study is to establish a molecular approach among the main commercial gastropod species in Chile. The mitochondrial genes 16S rRNA and COI, and the nuclear ribosomal...
Tipo: Journal article
Palavras-chave: 16S rRNA; COI; ITSl-5.8SrDNA-ITS2; Gastropod mollusks; Phylogenetic relationships.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382009000100003

Mitochondrial genetic variability of Didelphis albiventris (Didelphimorphia, Didelphidae) in Brazilian localities
Provedor de dados: 74
Autores: Sousa,Luciene C.C.; Gontijo,Célia M.F.; Botelho,Helbert A.; Fonseca,Cleusa G.
Didelphis albiventris is a well-known and common marsupial. Due to its high adaptability, this very widespread generalist species occurs under various environmental conditions, this even including protected regions and disturbed urban areas. We studied a 653 bp fragment of cytochrome oxidase c (COI) from 93 biological samples from seven Brazilian localities, with linear distances ranging between 58 and about 1800 km to analyze the effects of geographic distances on variability and genetic differentiation. The haplotype network presented nine haplotypes and two genetic clusters compatible with the two most distant geographic areas of the states of Minas Gerais, in the southeast, and Rio Grande do Sul, in the extreme south. As each cluster was characterized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Didelphis albiventris; Marsupial; Variability; COI; Genetic differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300020

Phylogenetic relationships and the evolution of mimicry in the Chauliognathus yellow-black species complex (Coleoptera: Cantharidae) inferred from mitochondrial COI sequences
Provedor de dados: 74
Autores: Machado,Vilmar; Araujo,Aldo M.; Serrano,José; Galián,José.
The phylogenetic relationships of twelve species of Chauliognathus were investigated by studying the mitochondrial cytochrome oxidase I gene. A 678 bp fragment of the COI gene was sequenced to test the hypothesis that the Müllerian mimicry species of the "yellow-black" complex make up a monophyletic clade, separated from species with other colour patterns. The data set was analysed by neighbour-joining, maximum parsimony and maximum likelihood procedures. The results support a single origin of the yellow-black colour pattern during the evolution of the genus, with one main clade formed by Chauliognathus lineatus, C. tetrapunctatus, C. riograndensis, C. flavipes, C. octomaculatus, C. fallax, and another one formed by two species, C. expansus and C sp 1,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cantharidae; Chauliognathus; COI; Coleoptera; Müllerian mimicry; Phylogeny; Yellow-black complex.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100010

A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes)
Provedor de dados: 74
Autores: Toffoli,Daniel; Hrbek,Tomas; Araújo,Maria Lúcia Góes de; Almeida,Maurício Pinto de; Charvet-Almeida,Patricia; Farias,Izeni Pires.
DNA barcoding is a recently proposed global standard in taxonomy based on DNA sequences. The two main goals of DNA barcoding methodology are assignment of specimens to a species and discovery of new species. There are two main underlying assumptions: i) reciprocal monophyly of species, and ii) intraspecific divergence is always less than interspecific divergence. Here we present a phylogenetic analysis of the family Potamotrygonidae based on mitochondrial cytochrome c oxidase I gene, sampling 10 out of the 18 to 20 valid species including two non-described species. Potamotrygonidae systematics is still not fully resolved with several still-to-be-described species while some other species are difficult to delimit due to overlap in morphological characters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potamotrygon; Barcoding; Radiation; Phylogenetics; COI; MtDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200028

Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion
Provedor de dados: 74
Autores: Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena.
Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033

Molecular evidence of two cryptic species of Stramonita (Mollusca, Muricidae) in the southeastern Atlantic coast of Brazil
Provedor de dados: 74
Autores: De Biasi,Juliana Beltramin; Tomás,Acácio Ribeiro Gomes; Hilsdorf,Alexandre Wagner Silva.
Abstract Snails of the genus Stramonita are commonly found in the rocky intertidal habitat of the western Atlantic Ocean coast. They belong to a monophyletic taxon that occurs along the tropical and warm-temperate Atlantic and eastern Pacific rocky shores. This genus comprises different valid species and members of the S. haemastoma complex. In the present study, samples of Stramonita were collected from three different regions of southeastern Brazil. Partial sequences of two mitochondrial genes, COI and 16S rRNA, were used to compare nucleotides sequences between Stramonita specimens. Levels of nucleotide divergence greater than 2% across the three sampled regions were used for differentiation at the species level. One of the identified species was S....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: COI; 16s rRNA; Mitochondrial DNA; Southern oyster drill; Brazilian coast.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300392

The history of the introduction of the giant river prawn, Macrobrachium cf. rosenbergii (Decapoda, Palaemonidae), in Brazil: new insights from molecular data
Provedor de dados: 74
Autores: Iketani,Gabriel; Pimentel,Luciana; Silva-Oliveira,Glaúcia; Maciel,Cristiana; Valenti,Wagner; Schneider,Horacio; Sampaio,Iracilda.
The giant river prawn, Macrobrachium cf. rosenbergii, is one of the most cultivated freshwater prawns in the world and has been introduced into more than 40 countries. In some countries, this prawn is considered an invasive species that requires close monitoring. Recent changes in the taxonomy of this species (separation of M. rosenbergii and M. dacqueti) require a re-evaluation of introduced taxa. In this work, molecular analyses were used to determine which of these two species was introduced into Brazil and to establish the geographic origin of the introduced populations that have invaded Amazonian coastal waters. The species introduced into Brazil was M. dacqueti through two introduction events involving prawns originating from Vietnam and either...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinvasion; Exotic species; Giant river prawn; Macrobrachium dacqueti; COI; 16S rRNA; Amazonia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100025

Presence of two mitochondrial genomes in the mytilid Perumytilus purpuratus: Phylogenetic evidence for doubly uniparental inheritance
Provedor de dados: 74
Autores: Vargas,Jaime; Pérez,Montse; Toro,Jorge; Astorga,Marcela P.
This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F genome was found in the somatic and gonadal tissues of females and in the somatic tissues of males; the M genome was found in the gonads and mantle of males only. For the mitochondrial 16S region, ten haplotypes were found for the F genome (nucleotide diversity 0.004), and 7 haplotypes for the M genome (nucleotide diversity 0.001), with a distance Dxy of 0.125 and divergence Kxy of 60.33%. For the COI gene 17 haplotypes were found for the F...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mytilidae; 16S; COI; Perumytilus; DUI.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200173

Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers
Provedor de dados: 74
Autores: Paim,Fabilene Gomes; Brandão,José Henrique Souza Galdino; Sampaio,Iracilda; Affonso,Paulo Roberto Antunes de Mello; Diniz,Débora.
Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI; Karyotype; South Atlantic fish; Ribossomal genes; Robertsonian rearrangements.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500007

Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home
Provedor de dados: 5
Autores: Benzoni, Francesca; Arrigoni, Roberto; Stefani, Fabrizio; Reijnen, Bastian T.; Montano, Simone; Hoeksema, Bert W.
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...
Tipo: Text
Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00145/25580/23718.pdf

Not all spotted cats are leopards: evidence for a Hemilienardia ocellata species complex (Gastropoda: Conoidea: Raphitomidae)
Provedor de dados: 5
Autores: Fedosov, Alexander E.; Stahlschmidt, Peter; Puillandre, Nicolas; Aznar-cormano, Laetitia; Bouchet, Philippe.
The small conoidean Hemilienardia ocellata is one of the easily recognizable Indo-Pacific "turrids", primarily because of its remarkable eyespot colour pattern. Morphological and molecular phylogenetic analyses revealed four species that share this "characteristic" colour pattern but demonstrate consistent differences in size and shell proportions. Three new species - Hemilienardia acinonyx sp. nov. from the Philippines, H. lynx sp. nov. from Papua New Guinea and H. pardus sp. nov. from the Society and Loyalty Islands - are described based on the results of phylogenetic analyses. Although the H. ocellata species complex clade falls in a monophyletic Hemilienardia, H. ocellata and H. acinonyx sp. nov. possess a radula with semi-enrolled or notably flattened...
Tipo: Text
Palavras-chave: Integrative taxonomy; Species delimitation; Indo-Pacific; COI; 16S.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00479/59033/61660.pdf

A new species of Phyllochaetopterus (Annelida: Chaetopteridae) from deep-sea hydrothermal Ashadze-1 vent field, Mid-Atlantic Ridge: taxonomical description and partial COI DNA sequence
Provedor de dados: 5
Autores: Morineaux, Marie; Nishi, Eijiroh; Ormos, Andrea; Mouchel, Olivier.
Phyllochaetopterus polus, a new species of the polychaete family Chaetopteridae is described. Specimens were collected at the Ashadze-1 vent field (Mid-Atlantic Ridge), from 4100-4200 m depth. P polus is the first chaetopterid reported from the Atlantic hydrothermal vents. Three chaetopterid species have been recorded from deep-sea hydrothermal vents belonging to two genera, Spiochaetopterus and Phyllochaetopterus. P polus sp. nov. is a medium-sized chaetopterid, characterized by 9 chaetigers in region A and 2 in region B; region C is usually incomplete in the specimens examined, but has at least 40-70 chaetigers. This polychaete has one pair of modified chaetae in chaetiger 4 with a pear-shaped obliquely truncated head, triangular uncini and a branched...
Tipo: Text
Palavras-chave: Polychaeta; Hydrothermal vent; Mid-Atlantic Ridge; Taxonomy; New species; COI.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12439/9250.pdf

First record of the coloured righteye flounder, Poecilopsetta colorata (Teleostei: Poecilopsettidae) from the Sakalaves seamounts in the Mozambique Channel
Provedor de dados: 5
Autores: Chen, Wei-jen; Chen, Jhen-nien; Pernet, Marie Eve Julie; Olu, Karine.
Background: The coloured righteye flounder, Poecilopsetta colorata Günther, 1880 was previously known from the eastern Indian Ocean to the South China Sea and Indonesia. Here, a new record from the western Indian Ocean is reported. Results: The new record is based on a specimen collected on the Sakalaves seamounts at 375 m in depth in the Mozambique Channel during a recent oceanographic survey. Four other teleost fish species including an uncommon ophidiid species, Neobythites somaliaensis Nielsen, 1995 were also collected on the same seamounts.  Conclusions: The presence of P. colorata in the Mozambique Channel suggests a broad and Indo-West Pacific wide distribution for this relatively rare deep-sea species. The sequence of the cytochrome oxidase...
Tipo: Text
Palavras-chave: Fishes; New record; Distribution; Western Indian Ocean; Mozambique Channel; Sakalaves seamounts; COI; PAMELA-MOZ01 cruise.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00353/46385/46009.pdf

Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)
Provedor de dados: 5
Autores: Arrigoni, Roberto; Richards, Zoe T.; Chen, Chaolun Allen; Baird, Andrew H.; Benzoni, Francesca.
Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...
Tipo: Text
Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00298/40912/39970.pdf

Phylogeny and phylogeography of Atlantic oyster species: evolutionary history, limited genetic connectivity and isolation by distance
Provedor de dados: 5
Autores: Lazoski, C.; Gusmao, J.; Boudry, Pierre; Sole-cava, A. M.
The phylogenetic relationships between naturally occurring Atlantic Crassostrea oyster species were inferred through analyses of mitochondrial (cytochrome oxidase subunit I and 16S) and nuclear (second internal transcribed spacer) sequences. We also scored 15 allozyme loci on 422 oysters to study population structuring of C. rhizophorae and C. brasiliana along 9000 km of the Western Atlantic coastline. Despite morphological similarities, C. virginica was genetically more closely related to C. rhizophorae than to C. brasiliana. In contrast, C. paraibanensis was genetically indistinguishable from C. brasiliana, which is probably a junior synonym of the African C. gasar. Significant genetic differentiation between populations of C. rhizophorae and C. gasar...
Tipo: Text
Palavras-chave: Population genetics; Biogeography; Allozymes; Cytochrome c oxidase subunit I; COI; 16S; Internal transcribed spacer 2; ITS-2.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14464/11765.pdf

Molecular phylogeny and evolution of the cone snails (Gastropoda, Conoidea)
Provedor de dados: 5
Autores: Puillandre, N.; Bouchet, P.; Duda, T. F., Jr.; Kauferstein, S.; Kohn, A. J.; Olivera, B. M.; Watkins, M.; Meyer, C.
We present a large-scale molecular phylogeny that includes 320 of the 761 recognized valid species of the cone snails (Conus), one of the most diverse groups of marine molluscs, based on three mitochondrial genes (COI, 16S rDNA and 12S rDNA). This is the first phylogeny of the taxon to employ concatenated sequences of several genes, and it includes more than twice as many species as the last published molecular phylogeny of the entire group nearly a decade ago. Most of the numerous molecular phylogenies published during the last 15 years are limited to rather small fractions of its species diversity. Bayesian and maximum likelihood analyses are mostly congruent and confirm the presence of three previously reported highly divergent lineages among cone...
Tipo: Text
Palavras-chave: Ancestral state reconstruction; Conidae; Conus; COI; 16SrRNA; 12SrRNA.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00468/57920/83480.pdf

Morphological and molecular characterization of Isostichopus sp. in the Colombian Caribbean sea
Provedor de dados: 46
Autores: Vergara,W; Agudelo,V; Castro,L; R. Rodríguez,A; Eeckhaut,I.
Isostichopus sp. (Aspidochirotida: Stichopodidae) are sea cucumbers widely distributed in the Caribbean Sea. Amongst them, Isostichopus badionotus is one of the most harvested species. It shows a wide range of morphotypes widespread in the Caribbean region including different habitats (muddy, sandy and rocky bottoms, and sea grass beds). In Colombia, three morphotypes can be distinguished; two of them live in sea grass beds while the third one is found on rocky substrates. The present study describes the morphological characteristics of these morphotypes and analyzes their genetic structures through 16S rDNA and COI data. Our phylogenetic analyses show that the morphotype living on rocky substrates is morphologically and genetically distinct from the two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucumbers; COI; 16S rDNA; Isostichopus badionotus.
Ano: 2018
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000200004

Searching for cryptic species in Erpobdella octoculata (L.) (Hirudinea: Clitellata): discordance between the results of genetic analysis and cross-breeding experiments
Provedor de dados: 16
Autores: Koperski, P.; Milanowski, R.; Krzyk, A.
The main aim of this study was to reveal reproduction barriers and potentially cryptic radiation within the very common and morphologically variable leech species - Erpobdella octoculata (L., 1758). The differences in reproductive success of the morphological forms were compared experimentally. The data based on the results of field sampling and analysis of ITS and COI sequences is also presented. The results of the analysis of DNA sequences clearly show lack of reproduction barriers between the analysed morphological forms. Subtle differences in DNA sequences between individuals, found with the use of the arbitrary primers method, seem to be related mainly to geographical distance between sub-populations. On the basis of these results, E. octoculata...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Cryptic species; Discordance; Hirudinea; ITS; Molecular taxonomy; 42.76.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/369712

Taxonomy, DNA barcoding and phylogeny of three new species of Pythium from Canada
Provedor de dados: 16
Autores: Bala, K.; Robideau, G.P.; Désaulniers, N.; Cock, A.W.A.M. de; Lévesque, C.A.
Three new species of Pythium, namely, P. oopapillum, P. emineosum and P. camurandrum are presented in this paper based on morphological descriptions and molecular phylogenetic characterisation. These new species were isolated from various ecological regions in Canada. They have unique morphological features in the genus Pythium, and form distinct clades in maximum parsimony analyses, which are also supported by maximum likelihood phylogeny using general time reversible model (GTR), and Bayesian inference (BI) phylogeny using Markov Chain Monte Carlo (MCMC) analysis methods. A comparative study of the new species with closely related taxa, their clade positions, and morphological features are described in this paper.
Tipo: Article / Letter to the editor
Palavras-chave: COI; ITS rDNA; LSU rDNA; Oomycete; Peronosporales; Pythium; Taxonomy.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/531810

Antispila oinophylla new species (Lepidoptera, Heliozelidae), a new North American grapevine leafminer invading Italian vineyards: taxonomy, DNA barcodes and life cycle
Provedor de dados: 16
Autores: Nieukerken, E. van; Wagner, D.; Baldessari, M.; Mazzon, L.; Angeli, G.; Girolami, V.; Duso, C.; Doorenweerd, C.
A grapevine leafminer Antispila oinophylla van Nieukerken &amp; Wagner, sp. n., is described both from eastern North America (type locality: Georgia) and as a new important invader in North Italian vineyards (Trentino and Veneto Region) since 2006. The species is closely related to, and previously confused with A. ampelopsifoliella Chambers, 1874, a species feeding on Virginia creeper Parthenocissus quinquefolia (L.) Planchon., and both are placed in an informal A. ampelopsifoliella group. Wing pattern, genitalia, and DNA barcode data all confirm the conspecificity of native North American populations and Italian populations. COI barcodes differ by only 0–1.23%, indicating that the Italian populations are recently established from eastern North America....
Tipo: Article / Letter to the editor
Palavras-chave: Invasive species; New species; Vitaceae; Viticulture; COI; Leafmines; Venation; Genitalia; Holocacista rivillei; 42.75.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/411010

Genetic and morphological variation in corallivorous snails (Coralliophila spp.) living on different host corals at Curaçao, southern Caribbean
Provedor de dados: 16
Autores: Potkamp, G.; Vermeij, M.J.A.; Hoeksema, B.W.
Snails of the genus Coralliophila (Muricidae: Coralliophilinae) are common corallivores in the Caribbean, feeding on a wide range of host species. In the present study, the morphological and genetic variation in C. galea and C. caribaea were studied in relation to their association with host coral species at Curaçao. Differences in shell shape among snails living on different hosts were quantified using geometric morphometric and phylogenetic relationships were studied using two mitochondrial markers (12S and COI). Based on these analyses, a new species, C. curacaoensis sp. nov., was found in association with the scleractinian coral Madracis auretenra. Both C. galea and C. caribaea showed host-specific differences in shell shape, size, and shell allometry...
Tipo: Article / Letter to the editor
Palavras-chave: Genetic differentiation; Phylogenetics; 12S; COI; Corallivory; Morphology; Geometric morphometrics; Host race.
Ano: 2017
URL: http://www.repository.naturalis.nl/record/630190

A molecularly based phylogeny reconstruction of mushroom corals (Scleractinia: Fungiidae) with taxonomic consequences and evolutionary implications for life history traits
Provedor de dados: 16
Autores: Gittenberger, A.; Reijnen, B.T.; Hoeksema, B.W.
The phylogenetic relationships of the Fungiidae, a family of predominantly free-living, zooxanthellate, reef corals, were studied by sequencing a part of the mitochondrial Cytochrome Oxidase I (COI) and the complete ribosomal Internal Transcribed Spacers (ITS) I &amp; II of specimens from various locations in the Indo- West Pacific. Some sequences were retrieved by using fungiidspecific primers on DNA-extracts from parasitic gastropods living with these corals. The analyses were performed both including and excluding intraspecific variation to investigate the potential effect of saturation. Even though the present molecular phylogeny reconstructions largely reflect those based on morphological characters, there are some distinct differences. Three major...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Evolutionary history; ITS 1 &amp;amp; 2; Maximum coral size; Polystomatism; Reproduction strategy; 42.72.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/376596

Hierarchical analysis of mtDNA variation and the use of mtDNA for isopod (Crustacea: Peracarida: Isopoda) systematics
Provedor de dados: 16
Autores: Wetzer, R.
Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent...
Tipo: Article / Letter to the editor
Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI.
Ano: 2001
URL: http://www.repository.naturalis.nl/record/534397

Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home
Provedor de dados: 16
Autores: Benzoni, F.; Arrigoni, R.; Stefani, F.; Reijnen, B.T.; Montano, S.; Hoeksema, B.
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA; 42.72.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/421558

Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)
Provedor de dados: 16
Autores: Arrigoni, R.; Richards, Z.T.; Chen, C.A.; Baird, A.H.; Benzoni, F.
Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision; 42.72.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/499033

Phytopythium: molecular phylogeny and systematics
Provedor de dados: 16
Autores: Cock, A.W.A.M. de; Lodhi, A.M.; Rintoul, T.L.; Bala, K.; Robideau, G.P.; Gloria Abad, Z.; Coffey, M.D.; Shahzad, S.; Lévesque, C.A.
The genus Phytopythium (Peronosporales) has been described, but a complete circumscription has not yet been presented. In the present paper we provide molecular-based evidence that members of Pythium clade K as described by Lévesque &amp; de Cock (2004) belong to Phytopythium. Maximum likelihood and Bayesian phylogenetic analysis of the nuclear ribosomal DNA (LSU and SSU) and mitochondrial DNA cytochrome oxidase subunit 1 (COI) as well as statistical analyses of pairwise distances strongly support the status of Phytopythium as a separate phylogenetic entity. Phytopythium is morphologically intermediate between the genera Phytophthora and Pythium. It is unique in having papillate, internally proliferating sporangia and cylindrical or lobate antheridia. The...
Tipo: Article / Letter to the editor
Palavras-chave: COI; LSU; Oomycetes; Oomycota; Peronosporales; Phytopythium; Pythiales; SSU.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/560055

The enigmatic Marmorkrebs (marbled crayfish) is the parthenogenetic form of Procambarus fallax (Hagen, 1870)
Provedor de dados: 16
Autores: Martin, P.; Dorn, N.J.; Kawai, T.; Heiden, C. van der; Scholtz, G.
A mysterious parthenogenetic cambarid crayfish (the Marmorkrebs) has been spreading across the globe for the past decade. We compare this crayfish directly to two other cambarids, Procambarus fallax and P. alleni, that have been suggested to be related or even identical to the Marmorkrebs. Using external morphology and sequences of two mitochondrial genes we show clear correspondences between Marmorkrebs and P. fallax, a species found natively throughout peninsular Florida, USA. Based on these congruent results we suggest that the Marmorkrebs is the parthenogenetic form of P. fallax. This finding has potential evolutionary and ecological implications at several levels. The Marmorkrebs might be a type of geographical parthenogenesis, but a natural...
Tipo: Article / Letter to the editor
Palavras-chave: 12S rRNA; Annulus ventralis; COI; DNA barcoding; Species concept; Thelytoky; 42.74.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/355212

Phylogenetic Relationships Among Species of the fraterculus Group (Anastrepha: Diptera: Tephritidae) Inferred from DNA Sequences of Mitochondrial Cytochrome Oxidase I
Provedor de dados: 82
Autores: SMITH-CALDAS,MARTHA R.B.; MCPHERON,BRUCE A.; SILVA,JANISETE G.; ZUCCHI,ROBERTO A.
A fragment of 808 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analyzed for 15 species of Anastrepha: 12 within the fraterculus group, one unplaced species and two outgroups. Phylogenetic relationships among the included taxa were inferred using neighbor-joining and maximum parsimony methods. The average Jukes-Cantor genetic distance among the species was 0.033±0.006 and the level of sequence divergence ranged from 0.0 to 0.083. Our results of COI indicate the placement of A. acris Stone, an unplaced species, in the fraterculus group. The membership of A. barbiellinii Lima in the fraterculus group and the monophyly of the aforementioned group are also discussed. Moreover, the presence of multiple gene pools in the nominal species A....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Insecta; Fruit flies; Anastrepha fraterculus; COI.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000400009

Molecules, wing pattern and distribution: an approach to species delimitation in the "loxurina group" (Lepidoptera: Lycaenidae: Penaincisalia)
Provedor de dados: 82
Autores: Prieto,C; Micó,E; Galante,E.
The wide range of morphological variations in the "loxurina group" makes taxa identification difficult, and despite several reviews, serious taxonomical confusion remains. We make use of DNA data in conjunction with morphological appearance and available information on species distribution to delimit the boundaries of the "loxurina" group species previously established based on morphology. A fragment of 635 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analysed for seven species of the "loxurina group". Phylogenetic relationships among the included taxa were inferred using maximum parsimony and maximum likelihood methods. Penaincisalia sigsiga (Bálint et al), P. cillutincarae (Draudt), P. atymna (Hewitson) and P. loxurina (C. Felder...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI; Eumaeini; Thecloxurina; Andes; Butterfly.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000500006

DNA barcoding confirms the occurrence of Rhamdia branneri and Rhamdia voulezi (Siluriformes: Heptapteridae) in the Iguaçu River Basin
Provedor de dados: 83
Autores: Ribolli,Josiane; Scaranto,Bianca Maria; Shibatta,Oscar Akio; Bombardelli,Robie Allan; Zaniboni-Filho,Evoy.
ABSTRACT DNA barcoding is a widely utilized molecular-based identification of species and taxonomic resolutions. Until recently, Rhamdia voulezi and Rhamdia branneri were considered species synonyms of Rhamdia quelen; however, morphological and cytogenetic analyses have suggested the validity of distinct species. Due to the absence of molecular taxonomy of R. voulezi and R. branneri, the objective of this study was to test its validity through traditional DNA barcoding and the GMYC (General Mixed Yule Coalescent) COI-based analyses in 19 specimens from the Iguaçu River Basin. In both methodologies, three MOTUs (Molecular Operational Taxonomic Units) were identified based on the estimated optimum threshold (OT = 0.77). The average inter-MOTU distance (NJ,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; COI; Jundiá; MOTU; Species complex.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100217

Cytogenetic and DNA barcoding reveals high divergence within the trahira, Hoplias malabaricus (Characiformes: Erythrinidae) from the lower Amazon River
Provedor de dados: 83
Autores: Marques,Diego Ferreira; Santos,Fabíola Araújo dos; Silva,Simoni Santos da; Sampaio,Iracilda; Rodrigues,Luís Reginaldo Ribeiro.
Molecular and cytogenetic data have provided evidence of cryptic speciation in the widespread South American trahira, Hoplias malabaricus. In the present study, karyotypes and DNA barcode sequences of specimens from seven populations inhabiting the lower Amazon River were analyzed in order to characterize the levels of genetic divergence within a single karyomorph. All the specimens presented karyotypes with 2n = 40 chromosomes (20m+20sm) that were consistent with the species' C karyomorph. The DNA barcodes revealed six haplogroups, with clear divergence between populations from Brazil and Argentina. The results support the species complex hypothesis and indicate that a single karyomorph of H. malabaricus may harbor more than one species
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon basin; COI; Karyomorph; Species complex; Trahiras.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200459

Phylogeography and conservation genetics of the Amazonian freshwater stingray Paratrygon aiereba Müller &amp;amp; Henle, 1841 (Chondrichthyes: Potamotrygonidae)
Provedor de dados: 83
Autores: Frederico,Renata G.; Farias,Izeni P.; Araújo,Maria Lúcia Góes de; Charvet-Almeida,Patricia; Alves-Gomes,José A.
The family Potamotrygonidae is monophyletic comprising three genera: Paratrygon Duméril, Potamotrygon Garman and Plesiotrygon Rosa, Castello &amp;amp; Thorson. The distribution of most species in this family is restricted to a single basin or fluvial system. Only Potamotrygon motoro, Potamotrygon orbignyi and Paratrygon aiereba are found in more than one river basin. In this study we investigate genetic structuring of Paratrygon aiereba, from five rivers of the Amazon region: Negro, Solimões-Amazon-Estuary system, Tapajós, Xingu and Araguaia. Sixty-three individuals were sequenced for ATPase 6, and a representative subsample of 27 individuals was sequenced for COI. The COI dataset analysis indicated that Paratrygon is sister to all other potamotrygonid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon basin; ATPase; COI; Population genetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100007

Morphometric variation of the Herichthys bartoni (Bean, 1892) species group (Teleostei: Cichlidae): How many species comprise H. labridens (Pellegrin, 1903)?
Provedor de dados: 83
Autores: Mejía,Omar; Pérez-Miranda,Fabián; León-Romero,Yatzil; Soto-Galera,Eduardo; Luna,Efraín de.
Cichlids of the tribe Heroini have long been a source of taxonomical conflict. In particular, the species included in the Herichthys bartoni group have failed to be recovered as monophyletic in different molecular studies. In this paper we use traditional and geometric morphometrics to evaluate morphological variation in the species included in the H. bartoni complex in order to evaluate the number of species it contains. An update of a previously published DNA barcoding study suggests the existence of three genetic clusters that included the six recognized species analyzed in this study, none of them recovered as monophyletic. On the other hand, geometric morphometrics arise as a useful tool to discriminate species due that traditional morphometrics...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cichlids; COI; DNA barcode; Geometric morphometrics; Mexico.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100061

Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family
Provedor de dados: 83
Autores: Hett,Anne Kathrin; Nirchio,Mauro; Oliveira,Claudio; Siccha,Zoila Raquel; Rossi,Anna Rita; Sola,Luciana.
This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; COI; 5S rDNA; Mullet; NORs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100008

Molecular identification of Atlantic goliath grouper Epinephelus itajara (Lichtenstein, 1822) (Perciformes: Epinephelidae) and related commercial species applying multiplex PCR
Provedor de dados: 83
Autores: Damasceno,Júnio S.; Siccha-Ramirez,Raquel; Oliveira,Claudio; Mendonça,Fernando F.; Lima,Arthur C.; Machado,Leonardo F.; Tosta,Vander C.; Farro,Ana Paula C.; Hostim-Silva,Maurício.
ABSTRACT The Atlantic goliath grouper, Epinephelus itajara , is a critically endangered species, threatened by illegal fishing and the destruction of its habitats. A number of other closely related grouper species found in the western Atlantic are also fished intensively. While some countries apply rigorous legislation, illegal harvesting followed by the falsification of fish products, which impedes the correct identification of the species, is a common practice, allowing the catch to be marketed as a different grouper species. In this case, molecular techniques represent an important tool for the monitoring and regulation of fishery practices, and are essential for the forensic identification of a number of different species. In the present study,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; COI; Endangered species; Fishery management; Forensic genetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000300203

REDESCRIPCIÓN DE POTAMOLITHUS SUPERSULCATUS PILSBRY, 1896 (GASTROPODA, TATEIDAE) DEL SUR DE LA CUENCA DEL PLATA
Provedor de dados: 84
Autores: LUCÍA,MICAELA DE; GREGORIC,DIEGO EDUARDO GUTIÉRREZ.
RESUMEN El género Potamolithus Pilsbry, 1896 (Gastropoda; Tateidae), posee 31 especies, 22 de las cuales se hayan en la Argentina distribuidas principalmente en la cuenca Del Plata, definiendo al río Uruguay y al Río de la Plata como una zona caliente de diversidad en gasterópodos dulceacuícolas. Sin embargo, la mayoría de sus especies han sido descriptas solo por caracteres conquilógicos y, unas pocas tienen datos anatómicos, conllevando a la descripción de subespecies o morfos que se superponen unos a otros. A Potamolithus lapidum algunos autores le atribuyen cuatro subespecies (con datos conquiológicos, y una con datos parciales de anatomía blanda), sin embargo otros incluyen ocho “morfos”. Aquí nosotros damos un comienzo en el estudio de Potamolithus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomía; COI; Conquilogía; Potamolithus lapidum; Especies vulnerables.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492017001700207

Heterobathmia pseuderiocrania Kristensen &amp;amp; Nielsen (Lepidoptera, Heterobathmiidae): identificación basada en DNA-barcoding y notas morfológicas e historia de vida de los estados inmaduros
Provedor de dados: 92
Autores: Ramos,Rosa A.; Parra,Luis E.
Heterobathmia pseuderiocrania Kristensen &amp;amp; Nielsen (Lepidoptera, Heterobathmiidae): identification based on DNA-barcoding and notes on the morphology and life history of the immature stages. The larva morphology of the species Heterobathmia pseuderiocrania (Lepidoptera, Heterobathmiidae), a Nothofagus obliqua leafminer in Chile, is described. The tissue-feeding first and last instars are described. Also, the number of larval stages, some aspects of the biology and life cycle of the species are provided.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biology; COI; Egg; Insecta; Morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000100005

Male and female association in Trichomyia Haliday in Curtis, 1839 using a molecular approach (Diptera, Psychodidae, Trichomyiinae), and description of new species from Brazil
Provedor de dados: 92
Autores: Araújo,Maíra Xavier; Aragão,Marcos; Cordeiro,Danilo; Bravo,Freddy; Carvalho,Claudio José Barros de; Andena,Sergio R.
ABSTRACT A new species of Trichomyia from the state of Bahia, Brazil, is described and illustrated, and male and female are associated using DNA barcoding. Additionally, fragments of the COI of two other species, Trichomyia cerdosa Araújo &amp; Bravo, 2016 and Trichomyia ituberensis Araújo &amp; Bravo, 2016, and the females of two unidentified species, are sequenced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA-barcoding; COI; Neotropical Region; Psychodid; Trichomyiinae.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000400283

The genus Basilichthys (Teleostei: Atherinopsidae) revisited along its Chilean distribution range (21° to 40° S) using variation in morphologyand mtDNA
Provedor de dados: 97
Autores: VÉLIZ,DAVID; CATALÁN,LAURA; PARDO,RODRIGO; ACUÑA,PATRICIO; DÍAZ,ANGIE; POULIN,ELIE; VILA,IRMA.
There is still doubt as to the number of species of the freshwater Chilean ichthyofauna, 64 % of which have conservation problems. One of the groups is that of the silversides of the genus Basilichthys. Three morphological species of this genus have been described in Chile with disjoint distributions: Basilichthys semotilus, B. microlepidotus and B. australis; the latter two overlap in distribution only in the Aconcagua River and are not easily distinguishable by morphological and meristic characters. In order to evaluate the efficacy of identification of these species by molecular techniques, we analyzed the sequence of 9 % of the mitochondrial DNA (Control Region and COI) of individuals from the Loa River (21°41' S) to the Valdivia River (39°50' S),...
Tipo: Journal article
Palavras-chave: COI; Control Region; Drainages; MtDNA; Silverside fish.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100004

Divergence time estimations and contrasting patterns of genetic diversity between Antarctic and southern South America benthic invertebrates
Provedor de dados: 97
Autores: GONZÁLEZ-WEVAR,CLAUDIO ALEJANDRO; DÍAZ,ANGIE; GERARD,KARIN; CAÑETE,JUAN IVÁN; POULIN,ELIE.
Diversity, abundance and composition of taxonomic groups in the Southern Ocean differ from elsewhere in the planet, since the biogeography in this region reflects the complex interactions of tectonics, oceanography, climate and biological elements since the Eocene. Several groups of marine benthic organisms exhibit high levels of genetic divergence among provinces in this region, supporting the existence of a vicariance process through plate tectonics, while other groups with high dispersive capacity exhibit recent divergence processes. Moreover, the discovery of non-Antarctic decapod larvae in Antarctic Peninsula suggests that some groups can travel across the Antarctic Circumpolar Current. Here we analyzed levels of genetic divergence in congeneric...
Tipo: Journal article
Palavras-chave: Antarctic Circumpolar Current; COI; Oceanographic barrier; Planktotrophy; Pliocene separation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000400007

DNA barcoding: a molecular appliance for fish species identification support
Provedor de dados: 22
Autores: Tresbach, Rafael Hencke; Cerqueira, Natalia Menezes; Medeiros, Sarah Ramos; Gutierrez, Harold Julián Pérez; Hernández, Natalia Ossa; Rodrigues, Marília Danyelle Nunes.
The identification of genus and species is made by systematic, by noticing morphological and anatomical characteres which were the phenotype expression. However, changes in one or more gene bases can result in phenotypes similarities between different species, inducing an identification error. Alternatively, molecular appliances based in mithocondrial and ribosomal genes appeared. This study aims to compile information about the molecular identification and present the South American Catfish (Rhamdia quelen) case, for which there is controversy generated by taxonomy identification. The different genes used to identification have their particularities, like insertions and deletions on the case of 16S rRNA and the 18S rRNA. Besides these ribosomal genes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA barcoding; Peixes; Rhamdia quelen; COI; DNA mitocondrial; DNA ribossomal.
Ano: 2015
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408

CARACTERIZACIÓN GENÉTICA DE LA POBLACIÓN DE Heliconius sara (Nymphalidae) EN LA ISLA GORGONA, COLOMBIA
Provedor de dados: 37
Autores: SEDAÑO CRUZ,Raúl Ernesto; CALERO MEJIA,Humberto.
RESUMEN La estructura genética de poblaciones de mariposas con distribución en islas y sus pares continentales ha sido poco documentada para el neotrópico. Este estudio presenta la caracterización de una población de Heliconius sara con distribución en la Isla Gorgona, ubicada en la región del Pacífico Oriental Colombiano. Para esto se examinaron secuencias parciales de un marcador mitocondrial incluyendo información obtenida del GenBank. Se comparó la diversidad y estructura genética con sus conespecíficos continentales y también con congéneres, con los que comparte un ancestro común cercano en el clado Sapho-Sara. Para el análisis de diversidad y estructura genética se realizó un análisis de varianza molecular. Este análisis muestra que la distancia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aislamiento por distancia; Filogeografía; Lepidóptera; COI; Mariposas.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000300374

DNA barcoding and traditional taxonomy unified through Integrative Taxonomy: a view that challenges the debate questioning both methodologies
Provedor de dados: 49
Autores: Pires,Amanda Ciprandi; Marinoni,Luciane.
The taxonomic crisis, emphasized in recent years, is marked by the lack of popularity (lack of interest in taxonomy) and financial incentives to study biodiversity. This situation, coupled with the issues involved with the necessity of knowing many yet undiscovered species, has meant that new technologies, including the use of DNA, have emerged to revitalize taxonomy. Part of the scientific community, however, has rejected the use of these innovative ideas. DNA barcoding has especially been the target of numerous criticisms regarding its application, as opposed to the use of morphology. This paper aims to highlight the inconsistency of the debate involving DNA versus morphology, since there is a proposal for the integration of traditional taxonomy and DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; COI; Species; Taxonomic impediment; Morphology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032010000200035

Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue
Provedor de dados: 49
Autores: Leite,Luis Anderson Ribeiro.
Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COI; Molecular; NUMTs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029

The Cytochrome Oxidase I gene confirms the presence of the nemertine Carcinonemertes conanobrieni, a parasite of the Caribbean spiny lobster (Panulirus argus) in Colombia
Provedor de dados: 50
Autores: Berben,Amanda; Gonzalez-Cueto,Jaime; Colorado,Angie; Quiroga,Sigmer.
ABSTRACT Morphological identifications complemented with molecular analysis are important for the delimitation of cryptic species. This document contains annotations on the molecular identification of the parasitic nemertine Carcinonemertes conanobrieni in the Caribbean spiny lobsters Panulirus argus. The collected nemerteans were found parasitizing ovigerous lobsters from the Gulf of Salamanca (Puebloviejo), Colombia. DNA was extracted from a specimen and a fragment of the mitochondrial gene Cytochrome Oxidase I (COI) was amplified using Folmer’s universal primers, the sequence was edited with ProSeq 3.5 and aligned with all the C. conanobrieni sequences available in GenBank. A sequence of 488 base pairs corresponding to the section of the genetic barcode...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcinonemertidae; COI; Puebloviejo.
Ano: 2022
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612022000100201

Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil
Provedor de dados: 52
Autores: Oliveira,Pablo Viana; Matos,Natan Silva; Klippel,Angélica Hollunder; Oliveira-Costa,Janyra; Careta,Francisco de Paula; Paneto,Greiciane Gaburro.
ABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on their morphology, new methods have been developed, including species identification by DNA barcodes. The objective of this study was to identify forensically important species of Diptera in Espirito Santo state using DNA barcodes. For this, adult flies were collected in Espirito Santo, Southeast Region of Brazil. After DNA extraction, COI gene was amplified and sequenced. All sequences were matched to BOLD platform and alternatively to GenBank MegaBLAST. As result,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomology; COI; BOLD; Forensic; Flies.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403