Consulta: subjectFacets:"Cell cycle arrest"
Registros recuperados: 4
Data/hora: 01/06/2024 02:25:47

Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells
Provedor de dados: 47
Autores: XU,Li-na; LU,Bi-nan; HU,Ming-ming; XU,You-wei; HAN,Xu; QI,Yan; PENG,Jin-yong.
Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining. The concentrations of lactate dehydrogenase and both acid and alkaline phosphatases were significantly increased in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Cell cycle arrest; Death receptor pathway; Mitochondrial pathway; Natural product.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300003

2-Methyl 2-butanol suppresses human retinoblastoma cells through cell cycle arrest and autophagy
Provedor de dados: 56
Autores: Li,Xiangyun; Zhu,Xiangxiang; Xu,Chong; Wu,Jianhua.
2-Methyl-2-butanol (MBT) is a chemical compound from the group of alcohols more specifically pentanols, which has shown an excellent anti-cancer activity in our previous study. However, its mechanism of action remains unclear. The present study was designed to investigate the anti-cancer effect of MBT on human retinoblastoma cells. The results showed that the use of MBT leads to HXO-RB44 cell death but is cytotoxic to normal cells at higher concentrations. It showed a dose- as well as a time-dependent inhibition of HXO-RB44 cells. P27 is a cell cycle inhibitory protein, which plays an important role in cell cycle regulation whereas cyclin-B1 is a regulatory protein involved in mitosis. MBT increased the cell cycle arrest in a dose-dependent manner by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-methyl-2-butanol; Retinoblastoma; Cell cycle arrest; Autophagy; Phosphoinositide 3-kinase (PI3K)/Akt.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500605

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells
Provedor de dados: 56
Autores: Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B.
Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines
Provedor de dados: 56
Autores: Park,S.H.; Sung,J.H.; Kim,E.J.; Chung,N.
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Berberine; Gemcitabine; Apoptosis; Cell cycle arrest.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200111