Consulta: subjectFacets:"Chlorhexidine"
Registros recuperados: 12
Data/hora: 15/06/2024 03:06:16

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction
Provedor de dados: 56
Autores: Bonacorsi,C.; Raddi,M.S.G.; Carlos,I.Z.
Chlorhexidine, even at low concentrations, is toxic for a variety of eukaryotic cells; however, its effects on host immune cells are not well known. We evaluated in vitro chlorhexidine-induced cytotoxicity and its effects on reactive oxygen/nitrogen intermediate induction by murine peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 ml of 10 mM phosphate-buffered saline, washed twice and resuspended (10(6) cells/ml) in appropriate medium for each test. Cell preparations contained more than 95% macrophages. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the presence of hydrogen peroxide (H2O2) and nitric oxide (NO) by the horseradish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Cytotoxicity; Macrophages; Nitric oxide; Hydrogen peroxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200007

Disinfection of gutta-percha cones with chlorhexidine
Provedor de dados: 58
Autores: Redmerski,Roberta; Bulla,Joice Renata; Moreno,Tatiana; Garcia,Lourdes Botelho; Cardoso,Celso Luiz.
This study investigated the effectiveness of detergent and aqueous solutions of 2% chlorhexidine digluconate in decontaminating gutta-percha cones (gpc) contaminated with bacteria, yeast, or bacterial spores. Gutta-percha cones were contaminated with 10(7)-10(8) colony-forming units per milliliter (cfu/ml) of the following test organisms: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Candida albicans. Spores of Bacillus subtilis were also tested. Contaminated gpc were treated with the chlorhexidine solutions for 1, 5, 10, or 15 min. Each cone was then transferred to a tube containing saline and the micoorganisms were recovered after homogenization for cfu determination. Both detergent and aqueous chlorhexidine solutions were effective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Decontamination; Gutta-percha cones.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400013

Levels of infection and colonization of some oral bacteria after use of naf, chlorhexidine and a combined chlorhexidine with naf mouthrinses
Provedor de dados: 58
Autores: Lima,Kenio Costa; Neves,Aline A.; Beyruth,Janine B.; Magalhães,Fernando A.C.; Uzeda,Milton de.
This study was carried out to evaluate levels of infection of bacteria involved in caries and oral streptococci and their colonization after use of mouthrinses with NaF, chlorhexidine and chlorhexidine plus NaF. Combination of chlorhexidine and fluoride was the only agent that reduced levels of Streptococcus mutans (group) for 30 days.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chlorhexidine; Sodium fluoride; Cariogenic bacteria; Oral streptococci.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200018

Sub-MIC of antibiotics induced biofilm formation of Pseudomonas aeruginosa in the presence of chlorhexidine
Provedor de dados: 58
Autores: Aka,Safaa T.; Haji,Sayran H.
Public health is facing a new challenge due to the alarming increase in bacterial resistance to most of the conventional antibacterial agents. It has been found that only minor cell damage is caused when exposed to sub-lethal levels of antimicrobial. Biofilms can play an important role in producing resistance, which is developed to reservoirs of pathogens in the hospital and cannot be easily removed. The aim of this study was to test whether the sub-lethal dose of antibiotics can induce biofilm formation of P. aeruginosafollowing incubating in the presence and absence of chlorhexidine. Standard antibiotic-micro broth 96-flat well plates were used for determination of MIC and biofilm assay. The adherence degree of biofilm was determined by estimation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic; Biofilm; Sub-MIC; Chlorhexidine; P. aeruginosa.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100149

Chlorhexidine: beta-cyclodextrin inhibits yeast growth by extraction of ergosterol
Provedor de dados: 58
Autores: Teixeira,K. I. R.; Araújo,P. V.; Sinisterra,R. D.; Cortés,M. E.
Chlorhexidine (Cx) augmented with beta-cyclodextrin (β-cd) inclusion compounds, termed Cx:β-cd complexes, have been developed for use as antiseptic agents. The aim of this study was to examine the interactions of Cx:β-cd complexes, prepared at different molecular ratios, with sterol and yeast membranes. The Minimal Inhibitory Concentration (MIC) against the yeast Candida albicans (C.a.) was determined for each complex; the MICs were found to range from 0.5 to 2 µg/mL. To confirm the MIC data, quantitative analysis of viable cells was performed using trypan blue staining. Mechanistic characterization of the interactions that the Cx:β-cd complexes have with the yeast membrane and assessment of membrane morphology following exposure to Cx:β-cd complexes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Β-cyclodextrin; Membrane-drug interactions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200047

Antinociceptive local effect of the combination of dexketoprofen trometamol and chlorhexidine gluconate in a formalin test: an additive effect
Provedor de dados: 60
Autores: Esparza-Villalpando,Vicente; Chavarria-Bolaños,Daniel; Zapata-Morales,Juan Ramón; Vértiz-Hernández,Antonio; Pozos-Guillén,Amaury.
ABSTRACT A drug delivery system (DDS) with analgesic and antibacterial properties would be desirable for the local control of post-operatory pain and the prevention for surgical site infection (SSI). The objective of the present study was to evaluate the antinociceptive effect of the combination between dexketoprofen trometamol (DXT) and chlorhexidine gluconate (CHX) in the formalin pain model. Different doses of CHX were combined with DXT and were locally administered in rats paw simultaneously with 5% formalin dilution. Flinches were documented and the antinociceptive effect was calculated. The area under the curve of each experimental group were calculated and the % of antinociception were compared. The groups of CHX and DXT showed similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception/effects; Formalin test; Dexketoprofen trometamol; Chlorhexidine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200601

Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution
Provedor de dados: 60
Autores: Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes.
Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017

Skin antisepsis protocols for the collection of blood from donor dogs
Provedor de dados: 65
Autores: Marchi,Melca Niceia Altoé de; Sargi,Lais Fernanda; Martins,Raquel Reis; Luz,Patrick Eugênio; Tanaka,Cristina Yuki; Pereira,Ulisses de Padua; Pereira,Patrícia Mendes.
ABSTRACT: The objective of this study was to evaluate and compare the bactericidal efficacy of2% chlorhexidine surfactant solution + 70% alcohol and 2% chlorhexidine surfactant solution + 0.5% chlorhexidine-alcohol, and standardize skin antisepsis for blood collection from donor dogs. One hundred and twenty skin swabsof the jugular regions of 20 dogs were evaluated. Swabs were distributed into six treatment(T) groups according to the disinfectant used and removal or retention of local hair: T1involved neither antisepsisnorhair removal; T2comprised 2% chlorhexidine + 0.5% chlorhexidine-alcoholwithout hair removal;T3 comprised 2% chlorhexidine + 70% alcohol without hair removal; T4comprised hair removal but no antisepsis;T5comprised 2% chlorhexidine + 0.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial contamination blood collection disinfection; Chlorhexidine; Dog.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500601

In-vitro Comparative Study of the use of 2 % Chlorhexidine on Microtensile Bond Strength of Different Dentin Adhesives: A 6 Months Evaluation
Provedor de dados: 77
Autores: Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
The aim of this study was to evaluate the effect of chlorhexidine (CHX) dentin treatment on microtensile bond strength (µTBS) of adhesive systems in different storage times. Occlusal enamel was removed from ninety third-molars and flat surfaces of middle dentin were exposed. Teeth were randomly divided in 6 groups according to adhesive system (etch-and-rinse : Adper Scotchbond 1XT - ASB ; self-etch: Adper Prompt L-Pop ­ APP; and universal: Single Bond Universal - SBU) and chlorhexidine (CHX) dentin treatment (2 % CHX application for 20 s prior Primer). After resin composite build up, teeth were sectioned to obtain beam specimens and divided in 3 subgroups (n=5): 72h, 3 and 6 months storage times. After the storage times, teeth were tested in tension until...
Tipo: Journal article
Palavras-chave: Microtensile bond strength; Dentin; Chlorhexidine; Dentin adhesives; Adhesion.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300016

Effect of 2 % Chlorhexidine on Dentin Shear Bond Strength of Different Adhesive Systems: A 6 Months Evaluation
Provedor de dados: 77
Autores: Bravo,Cristián; Sampaio,Camila S; Hirata,Ronaldo; Puppin-Rontani,Regina M; Mayoral,Juan Ricardo; Giner,Lluis.
This study evaluated the effect of chlorhexidine dentin treatment on shear bond strength (SBS) of adhesive systems after different storages. The work included 144 third molars that had their dentin exposed and were divided in 6 groups: G1 (ASB+CHX: Adper Scotchbond 1XT + chlorhexidine 2 % prior Primer); G2(ASB); G3 (APP+CHX: Adper Prompt L-Pop + CHX); G4(APP); G5 (SBU+CHX: Single Bond Universal + CHX); and G6(SBU). Resin build-up was performed and teeth were subdivided regarding storage times (n=8): 72 h, 3 and 6 months. Next, SBS test was performed. At 72 hours, all equivalent groups (same adhesive system, different dentin treatment) showed no significant difference in SBS (P.05). Self-etch adhesive groups (with or without CHX) presented lower SBS...
Tipo: Journal article
Palavras-chave: Shear bond strength; Dentin bond strength; Chlorhexidine; Adhesive systems; Adhesion.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300052

A Comparison of the Effect of Chlorhexidine, Tap Water and Normal Saline on Healing Wounds
Provedor de dados: 77
Autores: Salami,Ayodeji A; Imosemi,Innocent O; Owoeye,Olatunde O.
Many researchers have advocated in recent times that antiseptic use in healing wounds should be discouraged. Antiseptics have been found to retard healing of wounds. Innocuous solutions like normal saline and tap water are being considered as better alternatives to antiseptics. Chlorhexidine, a commonly used antiseptic, is known to be less toxic on granulation cells. We decided to compare the effect of chlorhexidine, normal saline and tap water on healing wounds. Three groups of wistar rats had 2 by 2cm full thickness wounds made on their right dorsolateral flanks. The wounds were dressed with either chlorhexidine, normal saline or tap water. Wounds were examined at three-day intervals and measurements of area covered were taken on the first day of study...
Tipo: Journal article
Palavras-chave: Chlorhexidine; Tap water; Nomal saline; Wound.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500025

Candida sp in the oral cavity with and without lesions: maximal inhibitory dilution of Propolis and Periogard
Provedor de dados: 59
Autores: Azevedo,Rosa Vitória Palamin; Komesu,Marilena Chinalli; Candido,Regina Celia; Salvetti,Cristiane; Rezende,Fausto Hanaoka Caetano.
Fifty individuals of both sexes aged on average 45.2 years were evaluated at the Semiology Clinic of FORP-USP in order to isolate and identify yeasts from the oral cavity, with and without lesions, and to determine the maximal inhibitory dilution (MID) of the commercial products Propolis (Apis-Flora) and Periogard (Colgate) against the strains isolated. Yeasts of the genus Candida were detected in the saliva of 9/19 (47.4%) individuals with a clinically healthy mouth, 18/22 (81.8%) of individuals with oral lesions, and in 4/9 (44.4%) of patients with deviation from normality, and were detected in 19/22 (86.4%) of the lesions. In the group with oral candidiasis, we isolated in tongue and lesion, respectively for each specie: C.tropicalis (8% and 10.7%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oral candidiasis; Propolis; Chlorhexidine; Yeasts.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400008