Consulta: subjectFacets:"Cleavage rate"
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Data/hora: 10/06/2024 00:49:45

Effect of insulin-like growth factor-1 during in vitro oocyte maturation and in vitro culture of bovine embryos
Provedor de dados: 45
Autores: Quetglas,M.D.; Coelho,L.A.; Garcia,J.M.; Oliveira Filho,E.B.; Esper,C.R.
The effects of insulin-like growth factor-I (IGF-I) on in vitro maturation (IVM) (experiment I) and on in vitro embryo development (experiment II) of bovine oocytes fertilized in vitro, were evaluated in terms of cleavage (CR), blastocyst (BR) and hatching (HR) rates. For IVM, immature cumulus-oocyte complexes were cultured in TCM-199 medium supplemented with Hepes, sodium bicarbonate, sodium pyruvate, additives, fetal calf serum (B-199 medium) and gonadotropins (14 U/ml PMSG and 7 U/ml hCG). For embryo development, the oocytes/zygotes were cultured in B-199 medium with bovine oviduct epithelial cells in suspension under silicon oil. Treatments for in vitro culture conditions for both experiments were: 1- B-199 + 200 ng/ml IGF-I; 2- B-199 + 100 ng/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Cleavage rate; IGF-I; In vitro maturation; In vitro fertilization.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200014