Consulta: subjectFacets:"Cloning"
Registros recuperados: 44
Data/hora: 05/06/2024 15:18:22

Identificación filogenética de Candidatus Liberibacter solanacearum y su efecto en la calidad fisiológica de la semilla de chile jalapeño.
Provedor de dados: 32
Autores: Olivares Mercado, Perla Xochitl.
En 1993 se reportó por primera vez en México a Candidatus Liberibacter solanacearum, como el agente causal de la pigmentación morada en las hojas, reducción de tamaño de la planta y necrosis interna de los tubérculos de la papa; posteriormente se detectó este bacterium en los cultivos de tomate, chile y berenjena. Sin embargo, la distribución de las plantas sintomáticas en el campo no tenía un patrón definido hasta que en 2009 se observó la totalidad de plantas atípicas de chile jalapeño cv Ixtapa en dos campos de los estados de Nayarit y Jalisco. Las plantas tuvieron una reducción de tamaño en un 30%, hojas con coloración verde pálido y deformación de los frutos en un 100%. Por tal razón la presente investigación tuvo como objetivos identificar el agente...
Palavras-chave: Chile jalapeño; Candidatus Liberibacter solanacearum; PCR; Clonación; Microscopia electrónica de barrido; Calidad de la semilla; Jalapeño pepper; Cloning; Electronic microscopy scanning; Seed quality; Producción de Semillas; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/1956

Variación intraespecífica del hongo conidiobolus coronatus y su relación endosimbiótica con la bacteria pseudomonas sp.
Provedor de dados: 32
Autores: Pérez Martínez, Carolina.
El hongo Conidiobolus coronatus es un saprófito facultativo, patógeno de muchas especies de insectos e incluso está reportado como causante de micosis subcutánea en humanos, aunque los reportes de esto último son poco comunes y se restringen a regiones con climas tropicales y de alta humedad. En la presente investigación se analiza la variación genética de aislamientos de C. coronatus de diferentes hospedantes (incluyendo al humano) y se corrobora la presencia de una bacteria endosimbiótica en dicho hongo. Mediante las técnicas RAPD, SSR y secuenciación de ITS 1 y 2 y región 5.8, se obtuvo variación intraespecífica y una clara distancia genética entre aislamientos obtenidos de humanos y otras fuentes (insectos y suelo). Para corroborar la endosimbiosis, se...
Palavras-chave: Análisis filogenético; Clonación; Endosimbiosis; Entomoftorales; Microscopía Láser confocal; Multihospedero; Polimorfismo; Variación intraespecífica; Phylogenetic analysis; Cloning; Endosymbiosis; Entomophtorales; Confocal lasser microscopy; Multihost; Polimorphism; Intraespecific variation; Doctorado; Entomología y Acarología.
Ano: 2010
URL: http://hdl.handle.net/10521/366

Cre-loxP recombination vectors for promoter studies
Provedor de dados: 69
Autores: Pedersen,Nina; Tuxen Poulsen,Thomas; Skovgaard Poulsen,Hans.
For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requires use of restriction enzymes and ligation reactions. One obstacle is the nucleotide polymorphisms of eukaryotic genomic DNA, which has the consequence that a sequence often differs from published sequences. Therefore sequencing, rigorous restriction enzyme analysis or introduction of suitable sites has to be performed prior to cloning and subcloning. In addition, conventional methods using restriction enzymes, insert purifications and ligations is expensive and labour demanding. We have developed a fast, efficient and inexpensive Cre recombinase-loxP based method, which allows cloning of promoter regions and subcloning of these into a variety of vectors in...
Tipo: Journal article
Palavras-chave: Cloning; Cre recombinase; Plasmid; Reporter genes; Therapeutic genes.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200013

Vaccines for finfish aquaculture: What do we need to know to make them work?
Provedor de dados: 69
Autores: Dixon,Brian.
Aquaculture still faces serious economic impacts due to the loss of animals to disease. A conservative estimate of 5% losses due to disease means that the finfish aquaculture industry loses over $1 billion annually on a global scale. One proven way to prevent costly disease outbreaks is to vaccinate fish against common or known pathogens. Current vaccination schemes still result in losses, however, and this may be due in part to vaccine design. Vaccines are currently designed using state of the art knowledge of immune responses, which is based primarily on mammalian studies. Just how applicable is this information to fish immunity and vaccine design, however? This review discusses what is currently known for teleost fish about two key processes that drive...
Tipo: Journal article
Palavras-chave: Antibody; Antigen presentation; Cloning; Cytokine; Immunity; Major histocompatibility complex receptors.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500014

LaNe RAGE: a new tool for genomic DNA flanking sequence determination
Provedor de dados: 69
Autores: Park,Daniel J.
The determination of genomic DNA sequence flanking a known region is often problematic. Existing technologies depend on multiple, efficient enzyme-catalysed preparative processing steps and/or rely on relatively inefficient ‘one-sided' PCR mechanisms. I demonstrate the application of a simple ‘two-sided' PCR-based approach, lariat-dependent nested PCR for rapid amplification of genomic DNA ends (LaNe RAGE), applied to the mouse GAPDH and PGK1 gene flanking sequences. This demonstration offers great promise in applications such as genome walking, transposon mutagenesis mapping and DNA fingerprinting
Tipo: Journal article
Palavras-chave: Cloning; DNA fingerprinting; Genomic DNA walking; PCR; Sequencing; Transposon mutagenesis.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200012

PCR assembly of synthetic human erythropoietin gene
Provedor de dados: 69
Autores: Bustami,Yazmin; Yahya,Ahmad Ramli Mohd; Muhammad,Tengku Sifzizul Tengku; Shu-Chien,Alexander Chong; Abdullah,Amirul Al-Ashraf; Noor,Mohd Azizan Mohd; Arip,Yahya Mat.
Human erythropoietin (huEPO) is a glycoprotein with important physiological functions, such as erythropoiesis, angiogenesis, and wound healing. A therapeutic protein, huEPO is commonly used to treat patients suffering from renal and non-renal anemia. Recombinant human erythropoietin (rhuEPO) and endogenous huEPO are similar with respect to their biological and chemical properties. In this study, we describe the construction of synthetic huEPO gene to produce rhuEPO. The synthetic huEPO gene was constructed by overlapping oligonucleotides assembly and amplified by polymerase chain reaction (PCR). Twenty oligonucleotide sets, covering the huEPO gene sequence and two newly introduced restriction enzyme sites, were pulled together and amplified using Pfu DNA...
Tipo: Journal article
Palavras-chave: Cloning; Erythropoietin; Oligonucleotide assembly.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300011

A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
Provedor de dados: 69
Autores: Jayashree,B; Buhariwalla,Hutokshi K; Shinde,Sanjeev; Crouch,Jonathan H.
Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different...
Tipo: Journal article
Palavras-chave: Cloning; Data mining; Drought avoidance; Drought tolerance; EST database; Root traits; Stress.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200002

Attitudes towards genomic: Research in four Latin American countries
Provedor de dados: 69
Autores: Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando.
The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,...
Tipo: Journal article
Palavras-chave: Cloning; Genome; Social representations; Transgenic.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003

Melhoramento genetico do guaranazeiro resultados de ensaios de avaliacao de clones fase produtiva 1985 a 1994.
Provedor de dados: 14
Autores: NASCIMENTO FILHO, F. J. do; ATROCH, A. L.; CRAVO, M. da S.
O objetivo e discriminar os clones quanto a produtividade e ao numero de colheitas baseado nos resultados referentes aos ensaios avancados, ou seja, aqueles que antecedem a recomendacao dos materiais, no periodo de 1985 a 1994. Para isso, foram realizadas analises de variancia individuais, para cada experimento, e uma analise de variancia conjuntas, para cada experimento, e uma analise combinada, envolvendo todos os clones, experimentos e anos.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Melhoramento genetico; Brasil; Amazonas; Manaus; Genetic gain; Cloning; Harvest.; Colheita; Clonagem; Guaraná; Paullinia Cupana.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/670823

Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes
Provedor de dados: 5
Autores: Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V.
The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No...
Tipo: Text
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf

Diversite de populations microbiennes thermophiles d'une cheminée hydrothermale océanique profonde : cultures d'enrichissement en bioréacteur et isolement d'espèces nouvelles
Provedor de dados: 5
Autores: Postec, Anne.
To explore the microbial diversity at deep sea vents, molecular techniques based on the analysis of the gene coding the 16S ribosomal RNA permitted to highlight a large archaeal and bacterial diversity. Nevertheless, the diversity of the hydrothermal microorganisms cultivated to date remains much lower than the diversity described with molecular tools. Innovative methods should therefore be used in order to cultivate new microorganisms, and also determine their metabolic properties and their potential role in the ecosystem. That was the purpose of this study: a bioreactor gas-lift was used to perform enrichment cultures in continuous, during 50 days, in thermophilic (60°C) and hyperthermophilic (90°C) conditions. A hydrothermal chimney sample (Rainbow...
Tipo: Text
Palavras-chave: Strain isolation and characterization; Hybridization; Sequencing; Cloning; DGGE; Gas lift bioreactor; Enrichment cultures; Deep sea hydrothermal vents; Bacteria; Archaea; Diversity; Isolement et caractérisation de souche; Hybridation; Séquençage; Clonage; DGGE; Bioréacteur gas lift; Cultures d'enrichissement; Sources hydrothermales océaniques profondes; Bacteria; Archaea; Diversité.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-367.pdf

Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique
Provedor de dados: 77
Autores: Venegas,Felipe; Guillomot,Michel; Vignon,Xavier; Servely,Jean-Luc; Audouard,Chistophe; Montiel,Enrique; Le Bourhis,Daniel; Perón,Sandrine; Soto,Pilar; Rojas,Mariana.
The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid...
Tipo: Journal article
Palavras-chave: Pudu deer; Cervidae; Ruminant; Cloning.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026

Arthropod venom Hyaluronidases: biochemical properties and potential applications in medicine and biotechnology
Provedor de dados: 80
Autores: Bordon,Karla C F; Wiezel,Gisele A.; Amorim,Fernanda G.; Arantes,Eliane C.
AbstractHyaluronidases are enzymes that mainly degrade hyaluronan, the major glycosaminoglycan of the interstitial matrix. They are involved in several pathological and physiological activities including fertilization, wound healing, embryogenesis, angiogenesis, diffusion of toxins and drugs, metastasis, pneumonia, sepsis, bacteremia, meningitis, inflammation and allergy, among others. Hyaluronidases are widely distributed in nature and the enzymes from mammalian spermatozoa, lysosomes and animal venoms belong to the subclass EC 3.2.1.35. To date, only five three-dimensional structures for arthropod venom hyaluronidases (Apis mellifera and Vespula vulgaris) were determined. Additionally, there are four molecular models for hyaluronidases fromMesobuthus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronidase; Scorpion; Spider; Caterpillar; Hymenoptera; Insects; Cloning; Heterologous expression; PEGylation; Biotechnological applications.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100214

Bradyrhizobium japonicumカタラーゼ遺伝子のクローニング方法の確立
Provedor de dados: 19
Autores: 白川, 雪香; 井川, 香子; 佐藤, 哲也; 大和田, 琢二; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya; OHWADA, Takuji.
Palavras-chave: Bradyrhizobium; カタラーゼ; クローニング; In vitro パッケージング; Bradyrhizobium; Catalase; Cloning; In vitro packaging.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1288

Stability analysis of chickpea large genomic DNA inserts in Agrobacterium
Provedor de dados: 30
Autores: Rajesh, P.N.
Palavras-chave: Cloning; Genomes; Plasmids; DNA; Chickpeas; Genetic transformation; Chromosomes; Antibiotics; Bacteria; Genes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3293

Sequence of ITS-2 Amplified from Pearl Millet Downy Mildew Samples
Provedor de dados: 30
Autores: Viswanathan, A.
Palavras-chave: DNA; PCR; Genetic techniques; Sampling; Ribosomes; Fungi; Genetic variation; Genes; Millets; Cloning.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3262

Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)
Provedor de dados: 30
Autores: Luu M Cuc.
Palavras-chave: Genomes; Genetic structures; Polymorphism; Alleles; DNA; Genotypes; PCR; Cloning; Locus; Groundnuts.
Ano: 2008
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3317

An effective method for cloning of partial MADS-box genes related to flower development in groundnut
Provedor de dados: 30
Autores: Yuan, M.
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213

Extending the repertoire of microsatellite markers for genetic linkage mapping and germplasm screening in chickpea
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genetic structures; Chickpeas; Genomes; Cloning; Chromosome mapping; Plasmids; Polymorphism; DNA; Genotypes; Genetic markers.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3338

Early selection and classification of yerba mate progênies
Provedor de dados: 86
Autores: Wendling,Ivar; Sturion,José Alfredo; Stuepp,Carlos André; Reis,Cristiane Aparecida Fioravante; Ramalho,Magno Antonio Patto; Resende,Marcos Deon Vilela de.
Abstract: The objective of this work was to evaluate the feasibility of early selection of open-pollinated yerba mate (Ilex paraguariensis) progenies, and to classify the best parents and candidates for clones. The germplasm is composed of 140 progenies collected from areas within the natural distribution of the species in Southern Brazil and a commercial genotype (control). The experiment was established in Ivaí, in the state of Paraná, Brazil, in March 1997, in randomized complete blocks, with ten replicates, and linear plots of six plants. The commercial mass (kg per plant) of leaves and branches smaller than 7 mm was harvested at 2.5, 4.5, 6.5, and 18.7 years of age. The statistical evaluation was performed using the mixed model procedure with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ilex paraguariensis; Cloning; Genetic correlation; Progeny x harvesting-age interaction.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300279

Preferences of US and EU Undergraduates for Cloning
Provedor de dados: 31
Autores: Anderson,   Shonda R.; Fox,   John A.
Poster Presentation
Tipo: Conference Paper or Presentation
Palavras-chave: Cloning; Consumer preferences; Agribusiness; Agricultural and Food Policy; Food Consumption/Nutrition/Food Safety.
Ano: 2010
URL: http://purl.umn.edu/61368

Consumer Preferences for Amount and Type of Fat in Ground Beef
Provedor de dados: 31
Autores: Lusk,   Jayson L.; Parker,   Natalie.
Scientists and beef industry participants are investigating ways to improve the healthiness of beef. We report results of a nationwide mail survey developed to determine consumers’ preferences for fat content in ground beef and identify how consumers would most like to improve the healthiness of beef. The results from a choice-based conjoint experiment indicate that consumers place significant value on reducing saturated fat and the Omega 6:3 ratio in ground beef, but were relatively unconcerned about conjugated linoleic acid. The relatively new method of best-worst scaling was used to further identify which methods consumers most preferred producers use to improve fat content in beef. The results indicate consumers preferred feeding cattle a grass-fed...
Tipo: Journal Article
Palavras-chave: Beef; Best-worst scaling; Cloning; Conjoint; Fat; Maximum-difference scaling; Omega 3 fatty acid; Agribusiness; Demand and Price Analysis; Food Consumption/Nutrition/Food Safety; Livestock Production/Industries; Marketing; M31; Q13.
Ano: 2009
URL: http://purl.umn.edu/48763

Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation
Provedor de dados: 90
Autores: Paixão,Douglas Antonio Alvaredo; Dimitrov,Mauricio Rocha; Pereira,Rodrigo Matheus; Accorsini,Fábio Raphael; Vidotti,Maria Benincasa; Lemos,Eliana Gertrudes de Macedo.
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Cloning; 16S rRNA; Metagenomics; Soil; Biosurfactants.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300019

Advances in the propagation of Jabuticaba tree
Provedor de dados: 94
Autores: Silva,José Antonio Alberto da; Teixeira,Gustavo Henrique de Almeida; Martins,Antonio Baldo Geraldo; Citadin,Idemir; Wagner Júnior,Américo; Danner,Moeses Andrigo.
Abstract  The Myrtaceae family has representatives spread worldwide, and Jabuticaba tree is an important species native to Brazil, which is grown not only in the backyards and domestic orchards, but also in commercial orchards, landscaping projects and in several tropical and subtropical countries. The propagation of this species in a sexual form or by seeds is simple and efficient, being able to reach 100%. However, long juvenile phase and genetic segregation have led to the search for methods that allow the propagation of selected trees and guarantee through their standardized and productive clones, fruit quality and fruiting precocity. Protocols for asexual propagation are still scarce, but they show efficient methods, making possible the commercial use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrtaceae; Plinia sp.; Grafting; Cutting; Air layering; Cloning.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000301007

Advances in the spread of vegetative blueberry
Provedor de dados: 94
Autores: Schuch,Márcia Wulff; Tomaz,Zeni Fonseca Pinto.
Abstract  The lack of an efficient method of propagation in some cultivars makes it difficult to expand the blueberry culture. In Brazil, we still do not produce seedlings with needed quality and quantity to improve the sector. The cloning of the selected material through mini propagation and micro propagation is an alternative to increase the multiplication and rooting capacity of the blueberries. Allied to this, the use of cultivation system without soil for the development of cloned material facilitates the seedlings production. In addition, we can keep matrices in small spaces with the use of mini-gardens and clonal micro-gardens controlling climatic and nutritional factors. In the Laboratory of Fruit Plants Propagation, of the Phytotechnology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seedlings; Cloning; Mini propagation and micro propagation; Cultivation system without soil.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000104001

MANGO PROPAGATION
Provedor de dados: 94
Autores: PINTO,ALBERTO CARLOS DE QUEIROZ; SAÚCO,VICTOR GALÁN; MITRA,SISIR KUMAR; FERREIRA,FRANCISCO RICARDO.
ABSTRACT  This Chapter has the objectives to search, through the review of the available literature, important informations on the evolution of mango propagation regarding theoretical and practical aspects from cellular base of sexual propagation, nursery structures and organizations, substrate compositions and uses, importance of rootstock and scion selections, also it will be described the preparation and transport of the grafts (stem and bud) as well as the main asexual propagation methods their uses and practices. Finally, pattern and quality of graft mangos and their commercialization aspects will be discussed in this Chapter.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cloning; Propagation; Propagation methods; Graft mangos.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000104001

Advances in vegetative propagation of Olive tree
Provedor de dados: 94
Autores: Schuch,Márcia Wulff; Tomaz,Zeni Fonseca Pinto; Casarin,Josiane Vergara; Moreira,Roseane Maidana; Silva,Jacqueline Barcelos da.
Abstract  In Brazil, there is a growing interest in the crop of the olive tree, especially in those regions considered suitable for cultivation, where low temperatures occur between 8ºC and 10ºC in the period before flowering. The areas with these characteristics are located mainly in the South and Southeast regions of the country, characterized by a temperate climate in function of altitude, with the occurrence of natural vernalization. The propagation is an integral part of the production chain and the first step towards the implantation of the olive tree crop or renovation of the existing orchards. Obtaining quality seedlings, besides guaranteeing uniformity and reliable varietal origin, is a factor that influences the whole life of the orchard,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seedlings; Cloning; Mini propagation and micro propagation; Cultivation system without soil.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201004

Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor subunit-1 in the hypothalamus of male sheep
Provedor de dados: 96
Autores: Ruiz,Ana Zuley.
The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cloning; NMDA receptor; Ovis aries; Ram; Sequencing.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183

การโคลนนิ่งและหาลำดับเบสของยีนที่ตอบสนองต่อสภาวะแล้งและความสัมพันธ์ระหว่างลักษณะโครงสร้างทางกายวิภาคกับการทนทานต่อสภาพแล้งในข้าวโพด  (Zea mays L.)
Provedor de dados: 106
Autores: Niran Juntawong; Prasart Kuermanee.
Drought stress could induce expressions of various genes that are involved in stress tolerance and responses. Recent studies were emphasized on a gene encoded for the 9-cis epoxycarotenoid dioxygenase (NCED) enzyme which catalyzes the biosynthesis of abscisic acid (ABA) in plants. However, little is known about drought tolerance genes in maize. We designed primers from the VP14 sequence in the NCBI gene bank for the study of expression of drought tolerance gene in SW 2301 drought tolerant and KSX 4605 drought sensitive lines. The RT-PCR showed an expression of drought responsible gene in SW 2301 drought tolerant line. Under drought condition, the expression of the responsible gene was higher in stem apical meristem than in root and leaf tissues. The cDNA...
Palavras-chave: Zea mays; Cloning; Sequencing; Drought responsible gene; Anatomical structure; Drought tolerance; 9-cis epoxycarotenoid dioxygenase; VP14; NCED; ข้าวโพด; พันธุ์; ยีนทนแล้ง; การโคลนนิ่ง; การหาลำดับเบส; โครงสร้างทางกายวิภาค; การทนแล้ง; กรดแอบไซซิก.
Ano: 2010
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4662

Caracterização molecular de dois isolados brasileiros de Lettuce mosaic virus apresentando propriedades biológicas distintas
Provedor de dados: 102
Autores: KRAUSE-SAKATE,RENATE; MELLO,RAQUEL N.; PAVAN,MARCELO A.; ZAMBOLIM,EUNIZE M.; CARVALHO,MURILO G.; LE GALL,OLIVIER; ZERBINI,F. MURILO.
Efetuou-se a clonagem e seqüenciamento do gene que codifica a proteína capsidial de dois isolados do vírus do mosaico da alface (Lettuce mosaic virus, LMV) provenientes do estado de São Paulo, previamente caracterizados como pertencentes aos patótipos II (AF198, incapaz de infetar cultivares com os genes de resistência mo1¹ ou mo1²) e IV (AF199, capaz de quebrar a resistência propiciada pelos genes mo1¹ e mo1²), com base na virulência em cultivares diferenciadoras. Análise comparativa das seqüências de nucleotídeos de isolados provenientes da Europa, América do Norte, Oriente Médio e os dois isolados brasileiros não permitiu sua separação em estirpes, pois as porcentagens de homologia foram sempre superiores a 95%. Entretanto, análise filogenética dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactuca; Lettuce; Potyvirus; LMV; Capsid protein; Cloning; Resistance.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000200006

Vegetative propagation of adult Ilex paraguariensis trees through epicormic shoots - doi: 10.4025/actasciagron.v35i1.15958
Provedor de dados: 1
Autores: Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária; Brondani, Gilvano Ebling; Universidade Federal de Mato Grosso; Biassio, Adriana de; Universidade Federal do Paraná; Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária.
The difficulty and length of time required for seed germination of mate (Ilex paraguariensis), as well as the pressing need for clonal multiplication of improved genetic material, has resulted in several studies related to vegetative propagation in an effort to obtain rooted cuttings more quickly and with better genetic quality. Currently, the biggest challenge is propagating and rooting adult plants selected in the field without requiring clear cutting to generate conditions for the basal induction of juvenile sprouts. Therefore, the objective of this study was to develop a method to rescue adult mate plants through the generation of epicormic sprouts. To accomplish this, tree branches of mate that were at least 19 years of age were collected and packed...
Palavras-chave: Mate; Shoot induction; Rhizogenesis; Cutting technique; Cloning; Vegetative propagation 5.01.03.04-0 mate; Shoot induction; Rhizogenesis; Cutting technique; Cloning; Vegetative propagation.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15958

Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii) - doi: 10.4025/actasciagron.v33i4.8317
Provedor de dados: 1
Autores: Brondani, Gilvano Ebling; Universidade Federal do Paraná; Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária; Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária; Grossi, Fernando; Universidade Federal do Paraná; Hansel, Fabricio Augusto; Empresa Brasileira de Pesquisa Agropecuária; Araujo, Marla Alessandra; Universidade Federal de Lavras.
This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg...
Tipo: Pesquisa
Palavras-chave: 5.01.03.04-0 in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3 Produção de Mudas in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/8317

Coherent soliton propagation through doped optical fibers: cloning, breakup, and soliton interactions
Provedor de dados: 42
Autores: CAVALCANTI,SOLANGE B.; FONSECA,EDUARDO J. DA S.; CAETANO,DILSON P.; HICKMANN,JANDIR M.
The simultaneous propagation of two optical pulses through a doped nonlinear dispersive medium modelled by a resonant three-level system was investigated numerically, within the framework of a pair of coupled extended nonlinear Schrödinger equations. These included the contribution of the dopant resonances whose dynamics is governed by Bloch equations. In this work, we review the interesting possibilities on the manipulation of fields such as cloning, breakup and soliton interactions, that the combination of coherent population trapping with nonlinear dispersive media offers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soliton; Quantum coherence; Nonlinearity; Cloning.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000200005

Molecular Cloning and Sequencing of AlkalophilicCellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Provedor de dados: 52
Autores: Walia,Abhishek; Mehta,Preeti; Guleria,Shiwani; Chauhan,Anjali; Shirkot,C.K.
ABSTRACT A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology withCellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulase free xylanase; Cellulosimicrobium cellulans; E.coli; Cloning; Gene.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600913

cDNA cloning, Phylogenic Analysis and Gene Expression Pattern of Phenylalanine ammonia-lyase in Sugarcane (Saccharum officinarum L.)
Provedor de dados: 52
Autores: Hashemitabar,Mahmoud; Kolahi,Maryam; Tabandeh,Mohammad Reza; Jonoubi,Parisa; Majd,Ahmad.
The aim of the present study was to clone and characterize a full length cDNA of sugarcane (Saccharum officinarum) phenylalanine ammonia-lyase (SoPAL). Differential tissue expression pattern of the SoPAL transcript and its enzyme activity was also analyzed during the tillering stage of growth. The full-length of SoPAL cDNA was 2118 bp long and contained a protein with 706 amino acids, determined by encoding technique. The amino acid sequence and phylogenic analysis of the cloned SoPAL showed high similarity to PAL from other monocotyledonous such as sorghum (96%), maize (93%) and Bamboos (87.12%). The highest levels of SoPAL transcript were observed in the root and stem, while its minimal gene expression levels were in the leaves and sheath, respectively....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenylalanine ammonia-lyase; Saccharum officinarumL; Cloning; Tissue expression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400456

Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli
Provedor de dados: 56
Autores: Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L.
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006

Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Provedor de dados: 56
Autores: Sato,N.S.; Suzuki,T.; Ueda,T.; Watanabe,K.; Hirata,R.D.C.; Hirata,M.H.
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell culture for GST-rTp47, GST-rTp17 and GST-rTp15, respectively. The identities of the antigens obtained were confirmed by automated DNA sequencing using ABI Prism 310 and peptide mapping by Finningan LC/MS. These recombinant antigens were evaluated by immuno-slot blot techniques applied to 137 serum samples from patients with a clinical and laboratory diagnosis of syphilis (61 samples), from healthy...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Treponema pallidum; Cloning; Recombinant antigen; Syphilis; GST.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700002

Production of a cloned calf from a fetal fibroblast cell line
Provedor de dados: 56
Autores: Mello,M.R.B.; Caetano,H.V.A.; Marques,M.G.; Padilha,M.S.; Garcia,J.F.; Milazzotto,M.P.; Assumpção,M.E.O.A.; Lima,A.S.; Nicácio,A.C.; Mendes,C.M.; Oliveira,V.P.; Visintin,J.A.
The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cloning; Nuclear transfer; Bovine; Fetal fibroblasts; Embryo reconstruction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100006

Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae
Provedor de dados: 56
Autores: Cui,Yubao; Zhou,Ying; Ma,Guifang; Yang,Li; Wang,Yungang; Shi,Weihong.
Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatophagoides farinae (Hughes); Der f 5; Cloning; Expression; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800009

Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
Provedor de dados: 58
Autores: Rulík,Martin; Chaudhary,Prem Prashant.
Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnetotactic bacteria; Sediment; PCR; Cloning; Phylogeny; 16S rRNA gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016

Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
Provedor de dados: 58
Autores: Zhu,Yanping; Pan,Jieru; Qiu,Junzhi; Guan,Xiong.
Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/&amp;#946;)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chitinase; Cloning; Overexpression; Homology modeling; Verticillium lecanii.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022

Rhizobacterial promotion of eucalypt rooting and growth
Provedor de dados: 58
Autores: Teixeira,Débora A.; Alfenas,Acelino Couto; Mafia,Reginaldo Gonçalves; Ferreira,Eraclides M.; Siqueira,Leandro de; Maffia,Luiz A.; Mounteer,Ann H.
A total of 107 rhizobacterial isolates, obtained from the rhizosphere of eucalypt clones were tested as rooting inducers of cuttings and mini-cuttings planted in substrate composed of carbonized rice husk and vermiculite (1:1). Cuttings and mini-cuttings were planted in conical plastic tubes containing treated and untreated (control) substrate and kept under intermittent mist irrigation at 26-28ºC. After 35 days, rooting percentage and dry root matter of cuttings were evaluated. Ten isolates capable of providing gains of up to 110% in root formation and up to 250% in root biomass over non-inoculated control cuttings were selected. Gains in rooting varied according to clone and isolate tested. The greatest gains were obtained for the mini-cuttings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; Cloning; Mini-cutting; Cutting; Rhizobacteria.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100025

Different alternatives for producing sprouts of avocado (Persea americana) rootstocks
Provedor de dados: 118
Autores: Castro,Mónica; Fassio,Claudia; Darrouy,Nicole; Aedo,Marcela; Jorquera,Lorena.
In Chile, most avocado orchards have been planted using 'Hass' as the scion variety on seedling rootstocks. This has led to the presence of a large variety of individuals, some of which show, among other characteristics, high productivity and a low degree of biennial bearing cycle in the same growing conditions. The most common method used in other countries to propagate such rootstocks from trees involves severe topping; however, this technique is notably destructive, making it difficult to employ in commercial orchards. With the goal of developing alternate methods for forcing shoot production from the rootstock, we tested two different procedures in July 2002 and March 2003, respectively: wounding in conjunction with the application of 1.1 M or 2.2 M...
Tipo: Journal article
Palavras-chave: Avocado; Basal sprout; Cloning; Cytokinin; Epicormic sprout; Girdling; Rootstock.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000200007

Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer
Provedor de dados: 65
Autores: Silva,Carolina Gonzales da; Martins,Carlos Frederico; Cardoso,Tereza Cristina; Cunha,Elisa Ribeiro da; Bessler,Heidi Christina; McManus,Concepta Margaret; Pivato,Ivo; Báo,Sônia Nair.
ABSTRACT: Wharton's jelly is a source of mesenchymal stem cells (MSCs) that had not yet been tested for bovine embryo production by nuclear transfer (NT). Thus, the objective of this study was to isolate, characterize and test MSCs derived from Wharton's jelly for embryo and pregnancy production by NT in cattle. The umbilical cord was collected during calving and cells derived from Wharton's jelly (WJCs) were isolated by explant and cultured in Dulbecco's Modified Eagle Medium. Skin Fibroblasts (FB) were isolated after 6 months of life. Morphological analysis was performed by bright field and scanning electron microscopy (SEM) during cell culture. Phenotypic and genotypic characterization by flow cytometry, immunocytochemistry, RT-PCR and differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cloning; Mesenchymal stem cell; Umbilical cord.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001830