Consulta: subjectFacets:"Clostridium perfringens"
Registros recuperados: 26
Data/hora: 01/06/2024 12:41:11

TOXIGENICITY CHARACTERIZATION OF Clostridium perfringens FROM BOVINE ISOLATES
Provedor de dados: 79
Autores: BALDASSI,L.; BARBOSA,M. L.; BACH,E. E.; IARIA,S. T.
Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Toxigenicity; Bovine isolates; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100010

Esterase electrophoresis of Clostridium perfringens bovine strains
Provedor de dados: 80
Autores: Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.
Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Esterase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200009

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India
Provedor de dados: 80
Autores: Rahman,Mafruza S; Sharma,RK; Borah,P; Chakraborty,A; Devi,Mandakini RK; Longjam,N.
Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens. Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. Among the isolated C. perfringens, 30 (62.50%) showed DNase production. Hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase C production. All the phospholipase C positive isolates revealed the presence of cpa gene encoding alpha (α) toxin. Of the 102...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Α toxin; Cpa gene; Polymerase chain reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100010

Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens
Provedor de dados: 80
Autores: Tolooe,A; Shojadoost,B; Peighambari,SM.
Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Necrotic enteritis; Chicken; Beta2 toxin; Cpb2; Iran.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100008

Virulence exaltation of Clostridium perfringens strains from bovines
Provedor de dados: 80
Autores: Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.
Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Virulence exaltation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300007

Detection of bovine Clostridium perfringens by polymerase chain reaction
Provedor de dados: 80
Autores: Piatti,R. M.; Ikuno,A. A.; Baldassi,L.
A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; PCR; Alpha toxin; Polymerase chain reaction.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005

Clostridium perfringens isolate typing by multiplex PCR
Provedor de dados: 80
Autores: Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB.
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006

Genotyping of Clostridium perfringens associated with sudden death in cattle
Provedor de dados: 80
Autores: Miyashiro,S; Baldassi,L; Nassar,AFC.
Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small intestine samples of bovines that have died suddenly, after manifesting or not enteric or neurological disorders. Two strains (8.7%) were identified as type E, two (8.7%) as type D and the remainder as type A (82.6%). Commercial toxoids available in Brazil have no label claims for efficacy against type E-associated enteritis; however, the present study shows the occurrence of this infection. Furthermore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Iota toxin; Sudden death; PCR; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300010

การปนเปื้อนของเชื้อจุลินทรีย์ที่ทำให้เกิดโรคในวัตถุดิบสำหรับผลิตน้ำพริกสำเร็จรูป และการศึกษาระยะเวลาในการอบเพื่อลดปริมาณ
Provedor de dados: 106
Autores: Siriporn Stonsaovapak; Pramote Tammarate; Kanchanich Vachanavinich.
Initial microbial loads of raw materials such as onion, garlic, dried shrimp, dried chilli and shrimp paste (kapi) that used in producing ready-made chilli paste were analysed. The results indicated that most of them contained high numbers of microorganisms. The total viable counts ranged from log10 CFU/g 4.279 – 7.778. The heaviest contamination was dried chilli. Escherichia coli were found between &lt;3 - 4.6 x 102 MPN/g. None of them was found to contain Salmonella spp. Staphylococcus aureus and Bacillus cereus were found ranging from log10 CFU/g 0 - 3.301 and 0 - 2.000, respectively. Clostridium perfringens was in the range of log10 CFU/g 1.000 – 4.623 and found in all kinds of raw materials, with high counts in dried chilli. After heat treatment at...
Tipo: PhysicalObject
Palavras-chave: Ready-made chilli paste; Raw materials; Reduction; Clostridium perfringens; น้ำพริกสำเร็จรูป; วัตถุดิบ; การลดปริมาณเชื้อจุลินทรีย์; เชื้อโรคในอาหาร; ระยะเวลาในการอบ; การปนเปื้อนเชื้อจุลินทรีย์; จุลินทรีย์ก่อโรค.
Ano: 1996
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5133

Production and purification of epsilon prototoxin produced by Clostridium perfringens type D
Provedor de dados: 45
Autores: Parreiras,P.M.; Lobato,F.C.F.; Heneine,C.F.L.G.D.; Assis,R.A.; Balsamão,G.M.; Nascimento,R.A.P.
A prototoxina epsilon foi obtida a partir de cultivo líquido, rico em carboidratos, a partir de amostra de C. perfringens tipo D (INTA - Argentina), incubado em condições de anarobiose à 37ºC por oito horas. O sobrenadante da cultura foi concentrado utilizando-se o sistema Amicon com membrana de "cut off" de 10 kDa. O concentrado foi precipitado com sulfato de amônio (350 g/ml) e dissolvido em tampão fosfato 0,01 M (pH 7,2). A prototoxina purificada por cromatografia de troca iônica (DEAE-Sepharose CL 6B) e isolada no primeiro pico, mostrou uma banda única de aproximadamente 34kDa de peso molecular em SDS-PAGE, além de ser tóxica em camundongos.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Enterotoxemia; Purification; Epsilon toxin; Ion exchange chromatography.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300019

Avaliação da resposta de antitoxinas beta e épsilon de Clostridium perfringens induzidas em bovinos e coelhos por seis vacinas comerciais no Brasil
Provedor de dados: 45
Autores: Lobato,F.C.F.; Moro,E.; Umehara,O.; Assis,R.A.; Martins,N.E.; Gonçalves,L.C.B.
Avaliou-se a resposta de antitoxinas beta e épsilon de Clostridium perfringens em bovinos vacinados contra clostridioses com seis vacinas disponíveis no mercado. Quarenta e oito bezerros de seis a sete meses de idade foram divididos em oito grupos (T1 a T8) de seis animais cada. Os grupos de número 2 a 7 receberam as vacinas T2 a T7 nos dias 0 e 42 com a dose e via recomendadas pelos fabricantes. Solução salina e toxóide padrão foram usados nos mesmos dias nos grupos 1 e 8 (T1 e T8), respectivamente, como controles negativo e positivo. Amostras de sangue foram coletadas nos dias 0, 42 e 56 pós-vacinação (PV), para titulação de anticorpos no soro. As vacinas e os controles foram também testados em oito coelhos cada, inoculados nos dias 0 e 21 com metade da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Coelho; Vacina; Clostridiose; Clostridium perfringens; Anticorpo antitoxina.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400004

Production and purification of beta-toxin from Clostridium perfringens type C
Provedor de dados: 45
Autores: Carvalho,A.V.A.; Heneine,L.G.D.; Assis,R.A.; Abreu,V.L.V.; Gonçalves,L.A.; Lobato,F.C.F.
Empregaram-se os métodos cromatográficos de afinidade metálica e de imunoafinidade para purificação da toxina beta em sobrenadante de cultivo de Clostridium perfringens tipo C. Observaram-se, na eletroforese das primeiras frações eluidas nos dois métodos de purificação, uma banda de peso molecular aproximado de 38kDa, característica da forma monomérica de toxina beta de Clostridium perfringens tipo C, e bandas de peso moleculares superiores, referentes às suas formas oligoméricas. Maior rendimento foi obtido com a utilização do método de imunoafinidade.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Clostridium perfringens; Beta-toxin; Purification; Metal affinity; Immunoaffinity.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000200018

Stability and toxicity of Clostridium perfringens type D epsilon prototoxin treated by iodine
Provedor de dados: 45
Autores: Rocha,P.H.; Assis,R.A.; Lobato,F.C.F.; Cardoso,V.N.; Heneine,L.G.D.
Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of stability of samples kept under different temperatures 4ºC, - 20ºC, and -80 ºC. The iodinated prototoxins were stocked for a period of four months.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Epsilon toxin; Iodination; Stability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000400007

Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis
Provedor de dados: 56
Autores: Ma,Yu-Hua; Ye,Gui-Sheng.
In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The RNA sequencing results showed that, compared with the control strain, 1128 genes were differentially expressed in the isolate strain, and these included 227 up-regulated genes and 901 down-regulated genes. Bioinformatics analysis identified the following genes and gene categories that are potentially involved in multidrug resistance (MDR) in the isolate strain: drug transport, drug response, hydrolase activity, transmembrane transporter,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Multidrug resistance; RNA sequencing; 2D-electrophoresis; Molecular mechanism.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800609

Antimicrobial susceptibility of Clostridium perfringens isolated from piglets with or without diarrhea in Brazil
Provedor de dados: 58
Autores: Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Pires,Prhiscylla Sadanã; Cruz Júnior,Eduardo Coulaud da Costa; Albefaro,Isabella Silva; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors related to the antimicrobial resistance of C. perfringens.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Piglet; Diarrhea; Antibiotic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300027

Cross protection by heat and cold shock to lethal temperatures in Clostridium perfringens
Provedor de dados: 58
Autores: García,Santos; Limón,Julio C.; Heredia,Norma L.
A heat shock pre-treatment of Clostridium perfringens provides protection against lethality from subsequent exposure to cold shock and vice versa. Heat shocked cells were more cold tolerant (one log reduction in 65 min) than control cells (one log reduction in 34 min). On the other hand, cold-shocked cells were more thermotolerant (D55= 17 min) than the control (D55= 6.5 min). The addition of chloramphenicol (an inhibitor of protein synthesis) in the experiments indicates that synthesis of new proteins was necessary for cross protection between both treatments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Heat shock; Cold shock.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200007

Antimicrobial activities of six essential oils commonly used as condiments in Brazil against Clostridium perfringens
Provedor de dados: 58
Autores: Radaelli,Marcela; Silva,Bárbara Parraga da; Weidlich,Luciana; Hoehne,Lucélia; Flach,Adriana; Costa,Luiz Antonio Mendonça Alves da; Ethur,Eduardo Miranda.
Abstract Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC–MS (gas chromatography–mass spectrometry). The identities of the isolated compounds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food-borne disease; Antimicrobial activity; Clostridium perfringens; Spices; Essential oils.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200424

Use of single-enzyme amplified fragment length polymorphism for typing Clostridium perfringens isolated from diarrheic piglets
Provedor de dados: 58
Autores: Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke.
Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034

Quality of water sources used as drinking water in a Brazilian peri-urban area
Provedor de dados: 58
Autores: Razzolini,Maria Tereza Pepe; Günther,Wanda Maria Risso; Peternella,Francisca Alzira dos Santos; Martone-Rocha,Solange; Bastos,Veridiana Karmann; Santos,Thaís Filomena da Silva; Cardoso,Maria Regina Alves.
The objective of this paper was to assess bacteriological quality of drinking water in a peri-urban area located in the Metropolitan Region of São Paulo, Brazil. A total of 89 water samples were collected from community plastic tanks and 177 water samples from wells were collected bimonthly, from September 2007 to November 2008, for evaluating bacteriological parameters including: Escherichia coli, Enterococcus and heterotrophic plate count (HPC). Clostridium perfringens was investigated in a subsample (40 samples from community plastic tank and 40 from wells). E. coli was present in 5 (5.6%) samples from community plastic tanks (2.0 - 5.1x10(4) MPN/100mL) and in 70 (39.5%) well samples (2.0 - 8.6x10(4) MPN/100mL). Thus, these samples were not in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Escherichia coli; Enterococcus; Peri-urban area; Drinking water.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200021

Jejunal hemorrhage syndrome in a Zebu cow in Brazil
Provedor de dados: 65
Autores: Pires,Prhiscylla Sadanã; Uribe,Jose Azael Zambrano; Carvalho,Antônio Último de; Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Meneses,Rodrigo Melo; Leite,Luiza Bossi; Alves,Guilherme Guerra; Gonçalves,Luciana Aramuni; Lobato,Francisco Carlos Faria; Facury Filho,Elias Jorge.
Clostridium perfringenstype A has been incriminated as the etiologic agent in jejunal hemorrhage syndrome (JHS), which is a disease that affects dairy cattle. Although this microorganism is considered an important enteropathogen the pathogenesis of JHS is still not clear, and there have been no reports of its occurrence in Brazil so far. The aim of this study was to describe the occurrence of JHS by infection with a C. perfringenstype A strain carrying the beta-2 toxin gene in a zebu cow in Brazil, for the first time.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Beta-2 toxin gene; Enteropathogen; Enteric blood clot.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801476

Clostridium perfringens em rações e águas fornecidos a frangos de corte em granjas avícolas do interior paulista: Brasil
Provedor de dados: 65
Autores: Schocken-Iturrino,Rubén Pablo; Vittori,Juliano; Beraldo-Massoli,Mariana Casteleti; Delphino,Tammy Priscilla Chioda; Damasceno,Priscila Regina.
Através de métodos bacteriológicos convencionais, avaliou-se a contaminação por Clostridium perfringens na ração e água utilizadas na alimentação e dessedentação de frangos de corte em diferentes regiões avícolas do interior paulista. C. perfringens esteve presente em 42 e 30% das amostras de ração e águas analisadas, respectivamente. As médias das contagens foram 6,7 x 10-2UFC mL para as amostras de água e 3,69 x 10-2UFC g para as de rações. As altas freqüências e contagens de C. perfringens verificadas nas rações e nas águas podem estar associadas à falta de higiene geral na manipulação e armazenamento dos mesmos. Sugere-se o monitoramento periódico da presença de C. perfringens nestas fontes, com a finalidade de evitar tal patógeno, em vista que o mesmo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alimento; Avicultura; Contaminação; Microbiologia; Qualidade; Clostridium perfringens.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000100033

Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D
Provedor de dados: 65
Autores: Freitas,Theonys Diógenes; Heneine,Luis Guilherme Dias; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Toxina épsilon; Anticorpos monoclonais.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100046

Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil
Provedor de dados: 65
Autores: Pinheiro,Carolina de Gouveia Mendes da Escóssia; Abrantes,Maria Rociene; Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Lobato,Francisco Carlos Faria; Silva,Jean Berg Alves da.
ABSTRACT:  The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium botulinum; Clostridium perfringens; Stingless bee.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900450

Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
Provedor de dados: 65
Autores: Ferreira,Thais Sebastiana Porfida; Moreno,Andrea Micke; Almeida,Renata Rodrigues de; Gomes,Cleise Ribeiro; Gobbi,Debora Dirani Sena de; Filsner,Pedro Henrique Nogueira de Lima; Moreno,Marina.
Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Swine; Slaughterhouse; PFGE; Carcass; Toxins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020

Alphitobius diaperinus spp como veiculador de Clostridium perfringens em granjas avícolas do interior paulista - Brasil
Provedor de dados: 65
Autores: Vittori,Juliano; Schocken-Iturrino,Rubén Pablo; Trovó,Kátia Prochnon; Ribeiro,César Augusto Martins; Barbosa,Gislaine Gomes; Souza,Luciano Melo de; Pigatto,Caroline Peters.
O besouro Alphitobius diaperinus spp (cascudinho) é visto como uma importante praga da avicultura mundial. Por suas características comportamentais e hábitos biológicos que dificultam seu controle, é considerado um vetor de agentes patogênicos. O objetivo desta pesquisa foi investigar o cascudinho como possível vetor de Clostridium perfringens em granjas avícolas industriais, localizadas em diferentes regiões do interior Paulista. Através de métodos bacteriológicos convencionais, em 40 amostras analisadas, foram encontradas contagens significativas de Clostridium perfringens em todas elas. A partir dos resultados obtidos, pôde-se demonstrar o potencial deste inseto como vetor do agente responsável pela enterite necrótica.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avicultura; Clostridium perfringens; Cascudinho; Enterite necrótica.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300048

Genotipicação de Clostridium perfringens isolados de frangos de corte através da PCR múltipla
Provedor de dados: 65
Autores: Gomes,Alexis de Matos; Lobato,Francisco Carlos Faria; Martins,Nelson Rodrigo da Silva; Assis,Ronnie Antunes de.
Clostridium perfringens (Cp) é uma bactéria aneróbica gram positiva que, além de provocar gangrena gasosa e enterotoxemia em humanos e animais, constitui-se na principal causa de enterite necrótica em aves de criações intensificadas. A identificação dos isolados foi realizada pela reação de lecitinase em ágar TSC-gema de ovo, colônias com dupla hemólise em ágar sangue desfibrinado de eqüino, coloração de Gram e provas bioquímicas. Das amostras analisadas, 171Cp foram isolados em jejuno e íleo de frangos de corte provenientes de um frigorífico da região de Pará de Minas-MG. Cp foi isolado em 62 (49,6%) de 125 amostras de conteúdo lumenal de jejunos e em 109 (87,2%) de igual número de íleos dos frangos de corte. Utilizando-se a técnica da PCR múltipla para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterite necrótica; Tipos toxigênicos; Frangos de corte; Clostridium perfringens.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000700022