Consulta: subjectFacets:"Coagulase-negative staphylococci"
Registros recuperados: 11
Data/hora: 05/06/2024 08:19:19

Risk of peritonitis during peritoneal dialysis in carriers of Staphylococcus aureus and coagulase-negative staphylococci
Provedor de dados: 80
Autores: Batalha,J. E. N.; Caramori,J. C. T.; Corrente,J. E.; Montelli,A. C.; Barretti,P.; Cunha,M. L. R. S.
The presence of Staphylococcus aureus in the nasal cavities and pericatheter skin of peritoneal dialysis patients put them at high risk of developing peritonitis. However, it is not clear whether the presence of coagulase-negative staphylococci (CNS) in the nasal passages and skin of patients is related to subsequent occurrence of peritoneal infection. The aim of the present study was to verify the relationship between endogenous sources of S. aureus and CNS and occurrence of peritonitis in patients undergoing peritoneal dialysis. Thirty-two patients on peritoneal hemodialysis were observed for 18 months. Staphylococcus species present in their nasal passage, pericatheter skin and peritoneal effluent were identified and compared based on drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Coagulase-negative staphylococci; Carriers; Peritoneal dialysis; Peritonitis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400005

Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
Provedor de dados: 80
Autores: Marconi,C.; Cunha,M. L. R. S.; Araújo Jr,J. P.; Rugolo,L. M. S. S.
Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Delta toxin; PCR; Staphylococcus; Coagulase-negative staphylococci.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200004

Bacterial biofilms with emphasis on coagulase-negative staphylococci
Provedor de dados: 80
Autores: Oliveira,A; Cunha,MLRS.
In addition to their capacity to attach to surfaces, various groups of microorganisms also produce an extracellular polymeric substance known as "slime". This slime forms a thin layer around cells known as biofilm. Thus, biofilm structure comprises bacterial cells and an extracellular polymeric substance. It also presents a defined architecture, providing the microorganisms with an excellent protective environment and favoring the exchange of genetic material between cells as well as intercellular communication. The ability to produce biofilm is observed in a large group of bacteria, including coagulase-negative staphylococci (CNS) which are the predominant microorganisms of normal skin flora and have been implicated as the causative agents of hospital...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; Biofilm; Slime; Infection; Microbiology.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400003

Inhibition of the growth of major mastitis-causing pathogens by non-aureus Staphylococcus isolates using the cross-streaking method
Provedor de dados: 45
Autores: Ferronatto,J.A.; Souza,F.N.; Libera,A.M.M.P. Della; Vliegher,S. De; Visscher,A. De; Piepers,S.; Blagitz,M.G.; Heinemann,M.B.
RESUMO O objetivo do presente estudo foi avaliar a capacidade de estafilococos não aureus (NAS) isolados de diferentes nichos ecológicos (leite, ambiente e ápice do teto), associados a vacas leiteiras, de inibir os principais agentes etiológicos da mastite bovina (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis e Escherichia coli). Neste estudo, 38 isolados NAS de diferentes nichos ecológicos foram avaliados quanto à capacidade de inibir o crescimento in vitro de importantes patógenos causadores de mastite pelo método cross-streaking. No total, 19 (50%) isolados de NAS (oito isolados de S. chromogenes, 10 de S. fleurettii e um de S. haemolyticus) apresentaram inibição contra os principais patógenos causadores de mastite. No entanto, a...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dairy cows; Coagulase-negative staphylococci; Intramammary infection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501745

Antimicrobial susceptibility of coagulase-negative staphylococci isolated from meat-producing ewes with mastitis
Provedor de dados: 45
Autores: Della Libera,A.M.M.P; Blagitz,M.G; Souza,F.N; Batista,C.F; Azedo,M.R; Benites,N.R; Melville,P.A; Gomes,V.
Avaliou-se a sensibilidade antimicrobiana in vitro de 121 cepas de estafilococos coagulase-negativa isolada de leite de ovelhas Santa Inês, aos fármacos: penicilina, amoxicilina, ampicilina, estreptomicina, oxaciclina, neomicina, cefalotina, gentamicina e sulfonamida. A resistência à sulfonamida foi a mais frequente (27,3%), seguida pela estreptomicina (14,0%) e pela oxaciclina (14,0%), enquanto da gentamicina (1,6%) foi a menos frequente. Todas as cepas foram sensíveis a pelo menos um antimicrobiano, e 20,3% das cepas apresentaram resistência múltipla. Os resultados mostram a importância de Staphylococci coagulase-negativas como agentes causadores de mastite em ovinos, e o perfil de resistência múltipla indica a importância da determinação da resistência...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ewe; Mastites; Coagulase-negative staphylococci.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600030

Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23
Provedor de dados: 55
Autores: Martínez-Meléndez,Adrián; Morfín-Otero,Rayo; Villarreal-Treviño,Licet; Camacho-Ortíz,Adrián; González-González,Gloria; Llaca-Díaz,Jorge; Rodríguez-Noriega,Eduardo; Garza-González,Elvira.
Abstract The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in Mexico, the Hospital Universitario in Monterrey and the Hospital Civil in Guadalajara. Antimicrobial susceptibility was determined. Biofilm formation was assessed by crystal violet staining. Detection of the ica operon and Staphylococcal Cassette Chromosome mec typing were performed by PCR. Clonal relatedness was determined by Pulsed-fiel gel electrophoresis and Multi locus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Linezolid resistance; Staphylococcal Cassette; Chromosome mec; Coagulase-negative staphylococci; Multilocus Sequence Typing.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500419

Oxacilin-resistant Coagulase-negative staphylococci (CoNS) bacteremia in a general hospital at São Paulo city, Brasil
Provedor de dados: 58
Autores: D'Azevedo,P.A.; Secchi,C.; Antunes,A.L.S.; Sales,T.; Silva,F.M.; Tranchesi,R.; Pignatari,A.C.C.
In the last decades, coagulase-negative staphylococci (CoNS), especially Staphylococcus epidermidis have become an important cause of bloodstream infections. In addition, rates of methicillin-resistance among CoNS have increased substantially, leading to the use of glicopeptides for therapy. The objective of this study was to evaluate eleven consecutives clinically relevant cases of oxacillin-resistant CoNS bacteremia in a general hospital localized in São Paulo city, Brazil. Five different species were identified by different phenotypic methods, including S. epidermidis (5), S. haemolyticus (3), S. hominis (1), S. warneri (1) and S. cohnii subsp urealyticus (1). A variety of Pulsed Field Gel Electrophoresis profiles was observed by macrorestriction DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; Bacteremia; Oxacillin; PFGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400006

Comparison between qualitative and semiquantitative catheter-tip cultures: laboratory diagnosis of catheter-related infection in newborns
Provedor de dados: 58
Autores: Marconi,Camila; Cunha,Maria de Lourdes RS; Lyra,João C; Bentlin,Maria R; Batalha,Jackson EN; Sugizaki,Maria Fátima; Rugolo,Lígia MSS.
This prospective study evaluated semiquantitative and qualitative catheter-culture methods for diagnosis of catheter-related infection (CRI) in newborns. Catheter tips from newborns admitted to the Neonatal Unit of the University Hospital of the Botucatu Medical School, UNESP were included in the study. Catheter cultures were performed with both semiquantitative and qualitative techniques. For CRI diagnosis, microorganisms isolated from catheter cultures and from peripheral blood cultures were identified and submitted to agent susceptibility test. The gold standard was the certain CRI diagnosis when same microorganism (specie and profile of susceptibility to agents) was isolated from both catheter tips and peripheral blood culture. A total of 85 catheters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catheter-related infection; Catheter culture; Semiquantitative culture; Newborns; Coagulase-negative staphylococci.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200012

Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens
Provedor de dados: 58
Autores: Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de.
Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010

Detection of enterotoxin genes of Staphylococcus sp isolated from nasal cavities and hands of food handlers
Provedor de dados: 58
Autores: Rall,V.L.M.; Sforcin,J.M.; Augustini,V.C.M.; Watanabe,M.T.; Fernandes Jr.,A.; Rall,R.; Silva,M.G.; Araújo Jr.,J.P.
Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcal enterotoxins; Coagulase-negative staphylococci; S. aureus; Food handler.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100011

Detection of enterotoxins genes in coagulase-negative staphylococci isolated from foods
Provedor de dados: 58
Autores: Cunha,Maria de Lourdes Ribeiro de Souza da; Peresi,Eliana; Calsolari,Regina Adriana Oliveira; Araújo Júnior,João Pessoa.
Staphylococcal food poisoning is caused by ingestion of enterotoxins preformed in the food contaminated essentially through human manipulation or raw material obtained from animals. Although coagulase-positive Staphylococcus aureus is the main agent responsible for food intoxication, some researches emphasise that coagulase-negative staphylococci (CNS) are able to produce staphylococcal enterotoxins and may be a potential cause of food poisoning. In the present study CNS were isolated from foods and the toxigenic capacity of the strains determined. A total of 88 food samples were analysed and 22.7% were positive for CNS strains. Staphylococcal counts ranged from 3.0 x 10² to 1.4 x 10(6) CFU/g or mL of food examined. S. epidermidis predominated among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coagulase-negative staphylococci; PCR; Enterotoxins.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100013