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Data/hora: 01/06/2024 12:32:22

Assessment of serological response of chickens to Salmonella Gallinarum and Salmonella Pullorum by Elisa
Provedor de dados: 89
Autores: Oliveira,GH de&lt;A NAME="nt01a"&gt;&lt;/A&gt;; Berchieri Júnior,Â; Montassier,HJ; Fernandes,AC.
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chickens; Conjugate; ELISA; Salmonella sp; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200007

A POLYCLONAL -ANTIBODY-IMMUNOPEROXIDASE-CONJUGATE FOR THE SPECIFIC DETECTION OF PORCINE CIRCOVIRUS TYPE 2
Provedor de dados: 101
Autores: Pérez,L.J.; Díaz de Arce,Heidy; Barrera,Maritza; Castell,Sara; Frías,María T.
Porcine circovirus 2 is nowadays accepted as the essential infectious agent of postweaning multisystemic wasting syndrome which causes severe economic losses in porcine production worldwide. The diagnosis of PMWS is a difficult task and must follow three criteria: (i) the presence of compatible clinical signs, (ii) the presence of characteristic microscopic histopathological lesions, and (iii) the presence of PCV2 within these lesions in moderate or high. The presence of PCV2 in lymphoid tissues must be demonstrated by in situ hybridization or immunohistochemical methods. The in situ hybridization is a more complex and expense compared to other diagnostic tools, on the other hand, one problem concerning the immunohistochemical methods for PMWS diagnostic...
Tipo: Journal article
Palavras-chave: Porcine circovirus 2; Immunohistochemical; Conjugate; Polyclonal antibody.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300003