Consulta: subjectFacets:"Copy number variation"
Registros recuperados: 6
Data/hora: 02/06/2024 11:40:06

Sequence diversity and copy number variation of Mutator-like transposases in wheat
Provedor de dados: 74
Autores: Asakura,Nobuaki; Yoshida,Shinya; Mori,Naoki; Ohtsuka,Ichiro; Nakamura,Chiharu.
Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutator-like transposase; Sequence diversity; Copy number variation; Triticum; Aegilops.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022

Human molecular cytogenetics: from cells to nucleotides
Provedor de dados: 74
Autores: Riegel,Mariluce.
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytogenetics to molecular genetics. This technique has a wide range of applications that increased the dimension of chromosome analysis. The field of cytogenetics is particularly important for medical diagnostics and research as well as for gene ordering and mapping. Furthermore, the increased application of molecular biology techniques, such as array-based technologies, has led to improved resolution,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular cytogenetics; FISH; Array-CGH; Copy number variation; Genomic disorders.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200006

Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco
Provedor de dados: 74
Autores: Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015

Nucleotide diversity and molecular evolution of the WAG-2 gene in common wheat (Triticum aestivum L) and its relatives
Provedor de dados: 74
Autores: Wei,Shuhong; Peng,Zhengsong; Zhou,Yonghong; Yang,Zaijun; Wu,Kai; Ouyang,Zhongming.
In this work, we examined the genetic diversity and evolution of the WAG-2 gene based on new WAG-2 alleles isolated from wheat and its relatives. Only single nucleotide polymorphisms (SNP) and no insertions and deletions (indels) were found in exon sequences of WAG-2 from different species. More SNPs and indels occurred in introns than in exons. For exons, exons+introns and introns, the nucleotide polymorphism Π decreased from diploid and tetraploid genotypes to hexaploid genotypes. This finding indicated that the diversity of WAG-2 in diploids was greater than in hexaploids because of the strong selection pressure on the latter. All dn/ds ratios were &lt; 1.0, indicating that WAG-2 belongs to a conserved gene affected by negative selection. Thirty-nine of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Genetic diversity; Molecular evolution; Triticum; WAG-2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400013

From the sxtA4 Gene to Saxitoxin Production: What Controls the Variability Among Alexandrium minutum and Alexandrium pacificum Strains?
Provedor de dados: 5
Autores: Geffroy, Solene; Lechat, Marc-marie; Le Gac, Mickael; Rovillon, Georges-augustin; Marie, Dominique; Bigeard, Estelle; Malo, Florent; Amzil, Zouher; Guillou, Laure; Caruana, Amandine.
Paralytic shellfish poisoning (PSP) is a human foodborne syndrome caused by the consumption of shellfish that accumulate paralytic shellfish toxins (PSTs, saxitoxin group). In PST-producing dinoflagellates such as Alexandrium spp., toxin synthesis is encoded in the nuclear genome via a gene cluster (sxt). Toxin production is supposedly associated with the presence of a 4th domain in the sxtA gene (sxtA4), one of the core genes of the PST gene cluster. It is postulated that gene expression in dinoflagellates is partially constitutive, with both transcriptional and post-transcriptional processes potentially co-occurring. Therefore, gene structure and expression mode are two important features to explore in order to fully understand toxin production processes...
Tipo: Text
Palavras-chave: Alexandrium; Saxitoxins; SxtA4; Copy number variation; SxtA; Expression; Isoform.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00682/79402/81944.pdf

Integrated analyses of copy number variations and gene differential expression in lung squamous-cell carcinoma
Provedor de dados: 48
Autores: Yang,Zhao; Zhuan,Bing; Yan,Ying; Jiang,Simin; Wang,Tao.
BACKGROUND: Although numerous efforts have been made, the pathogenesis underlying lung squamous-cell carcinoma (SCC) remains unclear. This study aimed to identify the CNV-driven genes by an integrated analysis of both the gene differential expression and copy number variation (CNV). RESULTS: A higher burden of the CNVs was found in 10-50 kb length. The 16 CNV-driven genes mainly located in chr 1 and chr 3 were enriched in immune response &amp;#91;e.g. complement factor H (CFH) and Fc fragment of IgG, low affinity Ilia, receptor (FCGR3A)&amp;#93;, starch and sucrose metabolism &amp;#91;e.g. amylase alpha 2A (AMY2A)&amp;#93;. Furthermore, 38 TFs were screened for the 9 CNV-driven genes and then the regulatory network was constructed, in which the...
Tipo: Journal article
Palavras-chave: Lung squamous-cell carcinoma; Differentially expressed genes; Copy number variation; Copy number variation-driven genes.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100047