Consulta: subjectFacets:"Cost effective"
Registros recuperados: 2
Data/hora: 16/06/2024 11:02:37

Universal protocol for generating 100bp size standard for endless usage
Provedor de dados: 69
Autores: Natarajan,Chandrasekhar; Vijayan,Neetha N; Vaidyan,Linda Koshy; Mathew,Anila; Srinivas,Lekshmy; Banerjee,Moinak.
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
Tipo: Journal article
Palavras-chave: 100bp ladder; Cost effective; DNA size standards; Endless usage.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015

Advancing Observation of Ocean Biogeochemistry, Biology, and Ecosystems With Cost-Effective in situ Sensing Technologies
Provedor de dados: 5
Autores: Wang, Zhaohui Aleck; Moustahfid, Hassan; Mueller, Amy V.; Michel, Anna Pm; Mowlem, Matthew; Glazer, Brian T.; Mooney, T. Aran; Michaels, William; Mcquillan, Jonathan S.; Robidart, Julie C.; Churchill, James; Sourisseau, Marc; Daniel, Anne; Schaap, Allison; Monk, Sam; Friedman, Kim; Brehmer, Patrice.
Advancing our understanding of ocean biogeochemistry, biology, and ecosystems relies on the ability to make observations both in the ocean and at the critical boundaries between the ocean and other earth systems at relevant spatial and temporal scales. After decades of advancement in ocean observing technologies, one of the key remaining challenges is how to cost-effectively make measurements at the increased resolution necessary for illuminating complex system processes and rapidly evolving changes. In recent years, biogeochemical in situ sensors have been emerging that are threefold or more lower in cost than established technologies; the cost reduction for many biological in situ sensors has also been significant, although the absolute costs are still...
Tipo: Text
Palavras-chave: In situ; Sensor; OceanObs; Ocean technology; EOVs; Biogeochemistry; Biology; Cost effective.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00514/62567/66921.pdf