Consulta: subjectFacets:"Culture media"
Registros recuperados: 33
Data/hora: 07/06/2024 08:07:12

Cultivo de morchella esculenta (L.) Pers. y obtención de esclerocios in vitro
Provedor de dados: 32
Autores: Alvarado Castillo, Gerardo.
Morchella esculenta es un hongo comestible de alto valor comercial y la obtención de  esclerocios es considerada como la clave para su cultivo. En este trabajo se evaluó  la cepa IE-750 en ocho medios de cultivo sólido y seis en forma líquida, utilizando  como parámetros el crecimiento micelial, la producción de biomasa, la habilidad para  producir esclerocios y su biomasa. El mejor crecimiento micelial se obtuvo en el  tratamiento con composta (T7: 53.87 cm2), y en cuanto a la biomasa, el medio de  cultivo con levadura fue mejor (T3: 80.3 mg). Los tratamientos con gallinaza y  composta, presentaron 40% y 80% de esclerocios, respectivamente, tanto en medio  sólido como líquido. La mayor cantidad de biomasa de estas estructuras se presentó  en el...
Tipo: Tesis
Palavras-chave: Esclerocios; Composta; Medios de cultivo; Morchella esculenta; Maestría; Agroecosistemas Tropicales; Sclerotia; Compost; Culture media.
Ano: 2008
URL: http://hdl.handle.net/10521/1414

Caracterización de las condiciones para la micropropagación de Polianthes tuberosa L. a partir de cormo, hoja, flor y yema.
Provedor de dados: 32
Autores: Hernández Mendoza, Fanny.
Debido a las tecnologías con que se cuenta en la actualidad es relativamente fácil incrementar la biodiversidad y se considera que dos grupos de técnicas son indispensables para aumentar la variabilidad genética de los cultivos. Las técnicas empleadas para la inducción de mutaciones son las herramientas útiles para incrementar la variabilidad genética y las técnicas de cultivo in vitro permiten la multiplicación rápida de los fenotipos seleccionados. El nardo (Polianthes tuberosa L.) es una planta endémica de México, la importancia de este cultivo radica en que es utilizada como flor de corte así como en la industria farmacéutica y del perfume. Es peculiar que presenta poca variabilidad genética, porque es propagada vegetativamente. En este estudio se...
Palavras-chave: Propagación; Regeneración de plantas; Medio de cultivo; Hormonas; Eficiencia de la regeneración; Contaminación; Regeneration of plantlets; Culture media; Hormones; Contamination; Genética; Maestría; Regeneration efficiency.
Ano: 2013
URL: http://hdl.handle.net/10521/2108

Caracterización de las condiciones para la micropropagación de Polianthes tuberosa L. a partir de cormo, hoja, flor y yema.
Provedor de dados: 32
Autores: Hernández Mendoza, Fanny.
Debido a las tecnologías con que se cuenta en la actualidad es relativamente fácil incrementar la biodiversidad y se considera que dos grupos de técnicas son indispensables para aumentar la variabilidad genética de los cultivos. Las técnicas empleadas para la inducción de mutaciones son las herramientas útiles para incrementar la variabilidad genética y las técnicas de cultivo in vitro permiten la multiplicación rápida de los fenotipos seleccionados. El nardo (Polianthes tuberosa L.) es una planta endémica de México, la importancia de este cultivo radica en que es utilizada como flor de corte así como en la industria farmacéutica y del perfume. Es peculiar que presenta poca variabilidad genética, porque es propagada vegetativamente. En este estudio se...
Palavras-chave: Propagación; Regeneración de plantas; Medio de cultivo; Hormonas; Eficiencia de la regeneración; Contaminación; Regeneration of plantlets; Culture media; Hormones; Regeneration efficiency; Contamination; Genética; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/2331

Production and characterization of algae extract from Chlamydomonas reinhardtii
Provedor de dados: 69
Autores: Kightlinger,Weston; Chen,Kai; Pourmir,Azadeh; Crunkleton,Daniel W; Price,Geoffrey L; Johannes,Tyler W.
Background: Algae offer many advantages as biofuel sources including: high growth rates, high lipid content, the ability to grow on non-agricultural land, and the genetic versatility to improve strains rapidly and produce co-products. Research is ongoing to make algae biofuels a more financially attractive energy option; however, it is becoming evident that the economic viability of algae-based fuels may hinge upon high-value co-products. This work evaluated the feasibility of using a co-product, algae extract, as a nutrient source in cell culture media. Results: Algae extract prepared from autolysed Chlamydomonas reinhardtii was found to contain 3.0% protein, 9.2% total carbohydrate, and 3.9% free &amp;#945;-amino acid which is similar to the nutrient...
Tipo: Journal article
Palavras-chave: Algae extract Algae; Chlamydomonas reinhardtii; Microalgae; Culture media.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100003

Asymbiotic germination in three Chloraea species (Orchidaceae) from Chile
Provedor de dados: 73
Autores: PEREIRA,GUILLERMO; ALBORNOZ,VERÓNICA; ROMERO,CHRISTIAN; LARA,SEBASTIÁN; SÁNCHEZ-OLATE,MANUEL; RÍOS,DARCY; ATALA,CRISTIAN.
ABSTRACT Orchids require symbiotic fungi and/or specific conditions to germinate. Asymbiotic techniques have been shown successful for orchid germination. In Chile, Chloraea include many endemic, and potentially ornamental, terrestrial orchid species. In this study, individuals of Chloraea crispa, C. gavilu and C. virescens were manually autopollinated. The resulting capsules were sterilized and seeds were aseptically obtained. We evaluated asymbiotic germination in: Agar Water (AW), Knudson C (KC), Banana Culture Media (CMB), Tomato Culture Media (CMT), Malmgren Modified (MM), Murashige and Skoog (MS), and MS modified (MS1/2). Seeds were incubated in the dark at 24±1 ºC for 2 weeks. Then they were put in 16/8 h light/dark cycles for 14 weeks. We...
Tipo: Journal article
Palavras-chave: Terrestrial orchids; Endemic species; In vitro germination; Culture media; Orchid propagation; Asymbiotic germination.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100131

Produção artificial de basidiocarpos de Crinipellis perniciosa do cupuaçuzeiro em meio de farelo-vermiculita.
Provedor de dados: 14
Autores: STEIN, R. L. B.; ITO, T.; ALBUQUERQUE, F. C. de; NASCIMENTO R. M. do.
bitstream/item/42901/1/Boletim-Pesquisa-167-CPATU.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Basidiocarpos; Theobroma agrandiflorium; Brasil.; Crinipellis Perniciosa; Cupuaçu; Fruta; Fungo; Meio de Cultura.; Amazonia; Culture media; Fruits; Fungi.
Ano: 1996
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/374452

Germinação "in vitro" de mogno.
Provedor de dados: 14
Autores: LOPES, S. da C.; LAMEIRA, O. A.; FORTES, G. R. L.; NOGUEIRA, R. C.; LEÃO, N. V. M.
bitstream/item/42026/1/BolPesquisa30.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Germinação in vitro; Pará; Brasil; Emergence.; Cultura de Tecido; Emergência; Mogno; Swietenia Macrophylla.; Substrato de Cultura; Amazonia; Culture media; In vitro fertilization; Tissue culture.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382708

Meios de cultura para esporulação do fungo Mycosphaerella fijiensis.
Provedor de dados: 14
Autores: HANADA, R. E.; PEREIRA, J. C. R.; GASPAROTTO, L.; COSTA, M. M. da.
Desenvolvimento de meios de cultura que, associados a regimes de luz, propiciem condicoes favoraveis para a producao massal de conidios de sigatoka negra, em Manaus-AM (Brasil).
Tipo: Outras publicações técnicas (INFOTECA-E)
Palavras-chave: Brasil; Amazonas; Manaus; Species; Fungal diseases.; Banana; Esporo; Espécie; Doença; Fungo; Meio de Cultura; Mycosphaerella Fijiensis.; Musa; Culture media; Spores.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/669760

Efeito de microorganismos benéficos no crescimento e nutrição mineral de mudas de tomate.
Provedor de dados: 14
Autores: YING, E. C.; BENCHIMOL, R. L.; MAKI, H. J. O.
bitstream/item/57305/1/CPATU-PA192.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Absorção de nutriente; Microorganismo; Controle de doença; Susbtrato de cultura; Pará; Brasil; Tomatos; Seedling; Growth; Nutient uptake.; Tomate.; Amazonia; Culture media; Disease control; Microorganisms.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/375199

Influência do tipo de substratos e de temperaturas na germinação de sementes de juquiri (Mimosa regnelii (i.e. regnellii) Benthan).
Provedor de dados: 14
Autores: FOWLER, J. A. P.; CARPANEZZI, A. A.
bitstream/CNPF-2009-09/16601/1/com_tec16.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Juquiri; Mimosa regnelii; Substrato; Propagação.; Espécie nativa; Germinação; Semente; Temperatura.; Culture media; Seeds; Temperature.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/290853

Influência do substrato no desenvolvimento de mudas de mogno Swietenia macrophylla, King.
Provedor de dados: 14
Autores: MARQUES, L. C. T.; YARED, J. A. G.; KANASHIRO, M.; BRIENZA JUNIOR, S.
bitstream/item/60174/1/CPATU-PA126.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Espécie florestal; Meio de cultivo; Plant development stages.; Etapa de Desenvolvimento da Planta; Mogno; Swietenia Macrophylla.; Culture media; Forest trees.
Ano: 1983
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/383255

Efeito do substrato e da adubacao no crescimento e na sobrevivencia de mudas de mangabeira em tubetes.
Provedor de dados: 14
Autores: PEREIRA, E. B. C.; PEREIRA, A. V.; PACHECO, A. R.
bitstream/CPAC-2009/24366/1/p2003_04.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Indigenous organisms.; Adubação; Areia; Cerrado; Fruta; Hancornia Speciosa; Mangaba; Substrato de Cultura.; Fertilizer application; Culture media; Sand.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/562985

Procedimentos para isolamento de bactérias que expressam sistemas alternativos de nitrogenases: Preparo de vidraria e meios de cultura.
Provedor de dados: 14
Autores: ALVES, G. C.; SILVA, M. F. da; FONSECA, W. C. da; TEIXEIRA, K. R. dos S.
Preparo de vidrarias e descartáveis. Preparo de meio de cultura livre de Mo. Preparo do meio Burk's 1x (concentração de trabalho). Validação da metodologia para o isolamento de bactérias que expressam sistemas alternativos de nitrogenases.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Mo.; Molibdênio.; Meio de Cultura; Culture media; Molybdenum.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/627702

Methods for microbial biomass, community structure and metabolic activities on surfaces.
Provedor de dados: 5
Autores: White, D.
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from...
Tipo: Text
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf

Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria.
Provedor de dados: 5
Autores: Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text
Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf

Effects of environmental conditions on the sessile existence of an estuarine sediment bacterium.
Provedor de dados: 5
Autores: Geesey, G; Salas, S; Mittelman, M.
A submerged glass coverslip technique was developed to determine dissolved organic nutrient uptake by adherent cells of a sediment isolate of Enterobacter cloacae . Cells which colonized the coverslips in swirling culture flasks during anaerobic growth remained firmly attached during manipulations employed to determine uptake of radiolabeled glucose by the adherent population. The attached cells were capable of a more rapid rate of glucose uptake than free cells. Lineweaver-Burk plots demonstrated different glucose uptake kinetics for the 2 cell populations. The data suggest that physiological changes occur in cells soon after they become attached to surfaces. These changes appear to enhance the metabolic activity of the adherent population.
Tipo: Text
Palavras-chave: Enterobacter cloacae; Adhesion; Glucose; Environmental effects; Bacteria; Sediments; Estuaries; Sessile species; Culture media; Metabolism; Dissolved oxygen; Microbiological analysis.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-967.pdf

A culture system for &lt;i&gt;Artemia, Daphnia&lt;/i&gt;, and other invertebrates, with continuous separation of the larvae
Provedor de dados: 27
Autores: Sorgeloos, P.; Persoone, G.
An easily constructed apparatus is described in which adult invertebrates can be reared with automatic feeding and with automatic and continuous separation of the offspring from the adults.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture media; Larvae.
Ano: 1973
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3471

Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra
Provedor de dados: 86
Autores: Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji.
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010

Salt concentrations in culture media for the development of Dipteryx alata in vitro
Provedor de dados: 86
Autores: Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de.
Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige &amp; Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295

In vitro germination and viability of pollen grain of coconut accessions1
Provedor de dados: 124
Autores: Moura,Catrine Regina Feitosa; Machado,Caroline de Araújo; Lédo,Ana da Silva.
Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; In vitro culture; Culture media.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000200421

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis
Provedor de dados: 63
Autores: Dibax,Roberson; Eisfeld,Cristiane de Loyola; Cuquel,Francine Lorena; Koehler,Henrique; Quoirin,Marguerite.
Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L-1 NAA and 4.44 µmol L-1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Forest species; Culture media; Organogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400016

Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes
Provedor de dados: 63
Autores: Onofre,Sideney Becker; Miniuk,Cindia Mara; Barros,Neiva Monteiro de; Azevedo,João Lúcio.
Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used....
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Metarhizium flavoviride; Mycelial growth; Culture media; Lighting regimes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000300026

การงอกและการเจริญเติบโตของเอมบริโอพืชในสกุล Prunus ที่ยังพัฒนาไม่เต็มที่โดยวิธีการเพาะเลี้ยงเอมบริโอ
Provedor de dados: 106
Autores: Nonglak Jeengool.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject
Palavras-chave: Peach; Prunus persica; Nectarine; Plum; Prunus domestica; Plant; Genus Prunus; Embryo; Germination; Growth; Embryo culture technique; Culture media; Woody plant medium; WPM; Brooks and Hough; BH; พีช; เนคทารีน; พลัม; พืช; เอมบริโอ; การงอก; การเจริญเติบโต; การเพาะเลี้ยงเอมบริโอ; อาหารเพาะเลี้ยง; สูตรอาหาร.
Ano: 2003
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5477

In vitro germination and growth of Vriesea incurvata Gaudich. (Bromeliaceae)
Provedor de dados: 109
Autores: Pulido-Rueda,Edwin Ernesto; Milaneze-Gutierre,Maria Auxiliadora; Negrelle,Raquel.
Abstract Vriesea incurvata in the natural environment shows some difficulties associated to the low seed germination capacity. Aiming to provide basis for the seedlings production, the results of the seeds germination percentage and the initial growth of V. incurvata seedlings in vitro conditions in different culture media, are reported. Completely randomized design was comprised of eight treatments and eight replications. The treatments were as culture media: MS (Murashige &amp; Skoog); MS%; KC (Knudson); KC%; MS + activated carbon (AC); MSV2 + AC; KC + AC; and KCV2 + AC. The germination percentage was calculated from the division between the number of seeds with primary root extrusion by the total number of sowed seeds. The initial growth was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Culture media; In vitro propagation; Native plant; Seed germination.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000100140

Effects of nutritional conditions on lipid production by cyanobacteria
Provedor de dados: 42
Autores: CORDEIRO,RAQUEL S.; VAZ,IZABELA C.D.; MAGALHÃES,SÉRGIA M.S.; BARBOSA,FRANCISCO A.R.
ABSTRACT The present study evaluated the effects of the culturing media and the levels of nitrogen and phosphorus on the growth, biomass productivity and lipid production of four species of Microcystis (M. novacekii, M. aeruginosa, M panniformis and M. protocystis). The lipid extract was obtained by refluxing with dichloromethane (Soxhlet). The biomass and biomass productivity yields were maximized with ASM-1 medium treatment enriched with nitrogen and/or phosphorus (0.25-0.65 g/L and 25-50.7 mg/L d-1, respectively). The lipid extract yields from M. panniformis and M. novacekii were inversely correlated with the concentration of nitrogen and directly correlated with the concentration of phosphorus (35.8 % and 31.7 %). The lipid extract yield from M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiesel; Culture media; Lipid extraction; Microcystis; Nitrogen and phosphorus deprivation and Stress.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502021

Antisepsys and profilatic antimicrobial therapy in prevention of surgical site infection of horses
Provedor de dados: 45
Autores: Canola,P.A.; Faria,A.P.P.A.; Paula,V.B.; Ávila,F.A.; Cardozo,M.V.; Rivera,G.G.
ABSTRACT The efficacy of an antisepsis protocol comprising chlorhexidine gluconate and ethyl alcohol in combination with prophylactic antimicrobial therapy in controlling surgical site infection in horses was studied. To that purpose, seven mixed breed horses received potassium penicillin and gentamicin at least 30 minutes prior to surgery. The surgical site was scrubbed with chlorhexidine gluconate and rinsed with ethyl alcohol. Samples were collected at four time points: (A) - before and (B) - immediately following shaving of the hair coat, (C) - at the end of antisepsis procedures, and (D) - at the end of the surgical procedure. Duration of surgery was recorded. Samples were cultured in three different culture mediums: Mitis Salivarus (Streptococcus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine gluconate; Culture media; Potassium penicillin; Staphylococcus sp; SSIs.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100049

Isolation and mycelial growth of Diehliomyces microsporus: effect of culture medium and incubation temperature
Provedor de dados: 52
Autores: Nascimento,José Soares do; Eira,Augusto Ferreira da.
The false truffle is one of the main problems in the production of the Agaricus brasiliensis in Brazil and the control of this fungal competitor has been rather difficult due to difficulties in the isolation and cultivation of this pathogen. This experiment was conducted in three stages, the first consisting of the isolation of Diehliomyces microsporus starting from portions of the fruiting body and through the ascospores suspension; second, D. microsporus cultivated in vitro at 15, 20, 25, 30 and 35ºC in six different culture media (CSDA, OCDA, PCDA, ODA, PDA, CDA); third, D. microsporus was inoculated on sterilized compost for formation of the fruiting body. The colony formation from tissue of D. microsporus starting from portions of fruiting body was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus brasiliensis; False truffle; Culinary-medicinal mushrooms; Culture media; Mushroom disease; Competitor.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400004

Growth capacity of thermotolerant campylobacters in culture media supplemented with pig and cow blood
Provedor de dados: 52
Autores: Tresierra-Ayala,Álvaro; Navas,Manuel; Flores,Josué; Perea,Ramsés; Huanaquiri,Juan; Bendayán,María; Fernández,Heriberto.
In this work 60 thermotolerant Campylobacter strains (37 C. jejuni and 23 C. coli) isolated from the cows, pigs, chickens and ducks (15 strains of each type of animal) were used to establish their growth capacity on media containing cow or swine blood as potential substitutes of sheep or horse blood. The growth capacity was assessed by viable counts on cow and swine blood media, using the modified Miles and Misra method. Campylobacter strains showed better growth in the media supplemented with pig or sheep blood than with cow blood. Thus, the use of pig blood could be a supplement for Campylobacter culture medium, when there was no availability of sheep or horse blood.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter; Culture media; Cow blood; Swine blood.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500011

Evaluation of gelling agents on anther culture: response of two soybean cultivars
Provedor de dados: 52
Autores: Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane.
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004

Assessment of the metabolism of different strains of Bacillus megaterium
Provedor de dados: 52
Autores: Suarez,Carlos Alberto Galeano; Montano,Inti Doraci Cavalcanti; Nucci,Edson Romano; Iemma,Monica Rosas da Costa; Giordano,Raquel de Lima Camargo; Giordano,Roberto de Campos.
Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic profile; Bacillus megaterium; Culture media.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001

Selective versus non-selective culture medium for group B streptococcus detection in pregnancies complicated by preterm labor or preterm-premature rupture of membranes
Provedor de dados: 55
Autores: Nomura,Marcelo Luís; Passini Júnior,Renato; Oliveira,Ulysses Moraes.
The objective of this study was to identify group B streptococcus (GBS) colonization rates and compare detection efficiency of selective versus non-selective culture media and anorectal versus vaginal cultures in women with preterm labor and preterm-premature rupture of membranes (PROM). A prospective cohort study of 203 women was performed. Two vaginal and two anorectal samples from each woman were collected using sterile swabs. Two swabs (one anorectal and one vaginal) were placed separately in Stuart transport media and cultured in blood-agar plates for 48 hours; the other two swabs were inoculated separately in Todd-Hewitt selective media for 24 hours and then subcultured in blood-agar plates. Final GBS identification was made by the CAMP test. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus agalactiae; Culture media; Premature rupture of membranes; Preterm labor.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400006

Growth of the Ectomycorrhizal Fungus Pisolithus Microcarpus in different nutritional conditions
Provedor de dados: 58
Autores: Rossi,Márcio José; Oliveira,Vetúria L.
The most important plant species employed in reforestation programs depend on ectomycorrhizal fungi for their establishment and growth. The exploitation of this symbiosis to improve forest productivity requires fungal inoculants in a large scale level. To develop such a technology it is necessary to define the optimal composition of the culture medium for each fungus. With these objectives in mind, the effect of the composition of the culture medium on biomass production of the ectomycorrhizal fungus Pisolithus microcarpus (isolate UFSC-Pt116) was studied. The original composition of two culture media, already employed for cultivation of ectomycorrhizal fungi, was submitted to several variations with the C/N ratio as the main variable. A variation of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pisolithus microcarpus; Culture media; C/N ratio; Submerged cultivation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200027

EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen
Provedor de dados: 58
Autores: Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p&lt;0. 0001; Fisher). Differences were found when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033