Consulta: subjectFacets:"Culture medium"
Registros recuperados: 26
Data/hora: 10/06/2024 10:16:15

Evaluación del efecto de diferentes diluciones de agua de coco suplementada con biotina, para la producción de levadura (Saccharomyces cerevisiae) a nivel matraz y en biorreactor de tanque agitado.
Provedor de dados: 32
Autores: Pérez Ramírez, Jacqueline.
En esta investigación, se evaluó el efecto de las diluciones del agua de coco y la adición de biotina sobre el crecimiento de Saccharomyces cerevisiae. En este sentido, la dilución 1:1 adicionada con biotina (µ= 0.21 h-1) y biomasa (3.32 g/L) fue suficiente para dar los requerimientos nutricionales a la cepa estudiada, en comparación con el agua de coco 100% (µ= 0.13 h-1) y biomasa (3.28 g/L) y un medio cultivo sintético (µ= 0.12 h-1) con biomasa de 2.86 g/L. Los nutrientes disponibles en diluciones 1:2 (µ= 0.18 h-1) y biomasa (2.25 g/L), podrían ser considerables para estudios posteriores; no así, las diluciones 1:5 y 1:10 que parecen inadecuadas para el óptimo crecimiento de S. cerevisiae. Se observó el efecto positivo de la biotina sobre el crecimiento...
Palavras-chave: Cepa; Medio de cultivo; Biomasa; Strain; Culture medium; Biomass production; Maestría Tecnológica; Agroindustria.
Ano: 2011
URL: http://hdl.handle.net/10521/643

Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil
Provedor de dados: 75
Autores: Pontes,Nadson C; Fujinawa,Miriam F; Oliveira,José R.
ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial wilt; Culture medium; Diagnosis; SMSA-E.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041

In vitro propagation of five Alocasia species
Provedor de dados: 75
Autores: Bhatt,Arvind; Stanly,Christine; Keng,Chan Lai.
The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige &amp;amp; Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alocasia amazonica; A. cuprea; A. robusta; A. longiloba; A. chaii; Culture medium; Acclimatization.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006

Agentes gelificantes alternativos na micropropagação de bananeira cv. Prata Catarina.
Provedor de dados: 14
Autores: SANTOS, H. M. G. dos; CARVALHO, A. C. P. P. de; SCHNEIDER, F.; ROSSETTI, A. G.; CAMPOS, A. S.
O ágar é o gelificante mais utilizado no preparo de meios de cultivo semissólidos na micropropagação vegetal. Porém, por ser um dos componentes de maior custo, agentes gelificantes alternativos vêm sendo prospectados, razão do objetivo deste trabalho ter sido avaliar o desempenho de mudas micropropagadas de ananeira, cv. Prata Cataria, na fase de alongamento e enraizamento, em meio de cultivo MS contendo diferentes gelificantes alternativos, tais como sagu, fécula de mandioca e amido de milho, produtos encontrados em supermercados. Agar is the most widely used gelling agent in the preparation of semi-solid culture media in plant micropropagation. However, because it is one of the most costly components, alternative gelling agents have been prospecting. The...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Agentes gelificantes; Meio de cultivo; Bananeira; Fécula de mandioca; Amido de milho; Maize starch; Culture medium; Musa sp; Micropropagação; Cassava starch; Gelling agents.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125505

Embryo rescue from interspecific crosses in apple rootstocks
Provedor de dados: 86
Autores: Dantas,Adriana Cibele de Mesquita; Boneti,José Itamar; Nodari,Rubens Onofre; Guerra,Miguel Pedro.
The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus; Marubakaido; M9; Culture medium; Carbon source.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600011

Crescimento in vitro de linhagens de coloração vermelha e verde clara de Gracilaria birdiae (Gracilariales, Rhodophyta) em dois meios de cultura: análise de diferentes estádios reprodutivos
Provedor de dados: 88
Autores: URSI,SUZANA; PLASTINO,ESTELA MARIA.
Gracilaria sp. é uma das principais macroalgas marinhas atualmente coletadas para produção de ágar no Brasil. Este trabalho objetivou comparar o crescimento in vitro de diferentes estádios reprodutivos tanto de linhagens selvagens (vermelhas), quanto de uma variante cromática (verde clara) de Gracilaria sp., em dois meios de cultura: Von Stosch e Provasoli. Seis linhagens foram selecionadas: gametófitos femininos (F) e masculinos (M), vermelhos (vm) e verde claros (vc); tetrasporófitos vermelhos, porém derivados de cruzamentos distintos, Fvm x Mvm e Fvc x Mvm. As taxas de crescimento avaliadas por 28 dias, foram calculadas a partir de medidas de massa fresca (TCm) e comprimento (TCc). A variante cromática apresentou TCm e TCc inferiores às verificadas para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gracilaria; Growth; Color strain; Reproductive stage; Culture medium.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500014

Collection time, dehidration, culture media and environment for germination and storage of campomanesia guazumifolia (cambess.) O. Berg. Pollen
Provedor de dados: 94
Autores: Guollo,Karina; Wagner Junior,Américo; Kosera Neto,Carlos; Radaelli,Juliana Cristina; Keller,Welida Mayara Tomazoni.
Abstract  Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce. The objective of this study was to elucidate aspects of pollen germination and viability of this specie. For the experiment, pollen from flowers in pre and post anthesis was used, which was dehydrated in a chamber containing silica, for different periods. For germination tests, different concentrations of sucrose, boric acid and calcium nitrate were used. After obtaining germination results above 80%, the pollen grains were stored in refrigerator (5 °C), freezer (-17 °C), liquid nitrogen (-147 °C) and natural environment (± 25 °C), evaluating monthly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Native fruit tree; Culture medium; Myrtaceae; Sete-capote tree.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452021000100803

Floral phase and sucrose/boric acid concentrations on in vitro germination of Inga edulis (Mart.), pollen grain
Provedor de dados: 22
Autores: Santos, Ana Claudia Schllemer dos; Castro, Juliana Dias de; Bressan, Daiane; Júnior, Américo Wagner; Guollo, Karina.
The aim of this work was to determine the ideal stage of pollen collection and the best concentrations of sucrose and boric acid in the culture medium for the in vitro germination of inga fruit tree pollen. For this, the work was carried out in two stages, one using concentrations of sucrose in the culture medium (0, 5, 10, 20 and 30%) - experiment 1. Another one using the best sucrose concentration, with concentrations (0, 100, 200 and 300 mg L-1) - experiment 2. In the two experiments, two flowering phases were use: pre-anthesis and post-anthesis. For both experiments, the culture medium contained 1% agar to solidify the medium and all treatments remained in BOD for 24 h at a temperature controlled at 25°C. For the experiment 1 with sucrose, DIC was use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture medium; Fabaceae; Native fruittree; Inga edulis Mart.; Pollen tube.
Ano: 2018
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/19619

In vitro seed germination and seedling development of Annona crassiflora Mart.
Provedor de dados: 63
Autores: Ribeiro,Márcia de Nazaré Oliveira; Pasqual,Moacir; Villa,Fabíola; Pio,Leila Aparecida Salles; Hilhorst,Henk William Maria.
Annona crassiflora Mart known as 'araticum', 'marolo' or 'field araticum' is a typical fruit from the Cerrado biome of Brazil with socio-economic and medicinal importance. Normally, Annona crassiflora is propagated through seeds. However, due to a deep dormancy that the seeds display at dispersion and the difficulty to obtain uniform plants in a short time period, micropropagation may be a feasible alternative. Concentrations of gibberellic acid (GA3) and naphthalene-acetic acid (NAA) and their interactive effects on in vitro seed germination and seedling development of Annona crassiflora were studied. Mature fruits of Annona crassiflora were depulped and the seeds washed in clear water and dried at room temperature. Seed coat was removed and the seeds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Culture medium; Growth regulators.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300017

In vitro propagation of Rubus macrocarpus Benth. and Rubus bogotensis Kunth, as an ex situ conservation strategy
Provedor de dados: 109
Autores: Pérez-Martínez,Belkys Adriana; Castañeda-Garzón,Sandra Liliana.
Abstract This research aimed to evaluate techniques for in vitro multiplication and rooting and ex vitro adaptation of wild blackberry plants (Rubus bogotensis Benth. and Rubus macrocarpus Kunth). In in vitro phases, the influence of culture media was evaluated based on Murashige &amp; Skoog (MS) composition, with or without a 50% reduction in salts, vitamins, and myo-inositol and supplemented or not with benzylaminopurine (BAP), indole acetic acid (IAA), L-cysteine, agar, peat, perlite, and vermiculite. The ex vitro phase was based on a soil, rice husks, peat, and perlite substrate management. After two months of evaluation of the in vitro phases, it was determined that the MS medium at 50% salts, vitamins, and myo-inositol and supplemented with sucrose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blackberry; Culture medium; High Andean forest; Plant growth regulator; Wild species.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000100102

Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino &amp;amp; E.C. Oliveira (Gracilariales, Rhodophyta)
Provedor de dados: 39
Autores: Ursi,Suzana; Guimarães,Melina; Plastino,Estela Maria.
This work evaluated the effects of Tris (hydroxymethyl)-aminomethane (TRIS) buffer and its interaction with nutrient concentration on the development of Gracilaria birdie, a common species on the Brazilian coast that has been exploited for agar production. Responses to different conditions were assessed through growth rates and pigment content (chlorophyll a, phycoerythrin, phycocyanin and allophycocyanin). Provasoli's nutrient solution with and without TRIS addition was tested at concentrations of 12.5, 25 and 50%. The pH was also monitored. G. birdiae grew better in the absence of TRIS and at low nutrient concentrations, 12.5 and 25% (growth rates of 10.8-11.3%.day-1). Higher contents of phycoerythrin and chlorophyll a were observed without TRIS at 12.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture medium; TRIS (hydroxymethyl)-aminomethane; Gracilaria birdiae; Growth; Pigments.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000300024

In vitro cultivation of zygotic embryos from Murici (Byrsonima cydoniifolia A. Juss.): establishment, disinfection, and germination - doi: 10.4025/actasciagron.v35i2.15402
Provedor de dados: 1
Autores: Martendal, Cíntia de Oliveira; Universidade Estadual de Maringá; Bernardino, Murilo Martins; Instituto Federal Goiano; Pereira, Flávia Dionísio; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Silva, Fabiano Guimarães; Instituto Federal Goiano; de Menezes, Carlos César Evangelista; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Monteiro Hara, Alessandra Cristina Boffino de Almeida; Instituto Federal Goiano.
The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid...
Tipo: Pesquisa Empírica de Campo
Palavras-chave: 5.01.03.04-0 tissue culture; Asepsis; Culture medium; Cerrado fruit Fitotecnia.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15402

Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii) - doi: 10.4025/actasciagron.v33i4.8317
Provedor de dados: 1
Autores: Brondani, Gilvano Ebling; Universidade Federal do Paraná; Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária; Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária; Grossi, Fernando; Universidade Federal do Paraná; Hansel, Fabricio Augusto; Empresa Brasileira de Pesquisa Agropecuária; Araujo, Marla Alessandra; Universidade Federal de Lavras.
This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg...
Tipo: Pesquisa
Palavras-chave: 5.01.03.04-0 in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3 Produção de Mudas in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/8317

Fontes de silício na micropropagação de orquídea do grupo Cattleya - doi: 10.4025/actasciagron.v33i3.6281
Provedor de dados: 1
Autores: Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras.
O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 &amp;plusmn; 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121&amp;deg;C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 &amp;micro;mol m-2 s-1, temperatura de 25 &amp;plusmn; 1&amp;deg;C e...
Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281

Morphological and physiological photon flux influence under in vitro culture of apple shoots
Provedor de dados: 52
Autores: Zanandrea,Ilisandra; Bacarin,Marcos Antonio; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio.
The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21 and 60 µmol m-2 s-1) and three culture media. The best photon flux for maximizing growth, number of shoots and leaves was 14 µmol m-2 s-1, which also resulted in the highest values of chloroplastic pigments. On the other hand, the highest photon flux (60 µmol m-2 s-1) decreased significantly these parameters. Medium with reduced sucrose concentration did not affect the analyzed parameters, enabling an efficient multiplication of the cultivar with only 1% of sucrose.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mallus; Apple; Sucrose; Photon flux density; Culture medium.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500005

Production of outer membrane vesicles (OMV) in batch cultivation of Neisseria meningitidis serogroup B
Provedor de dados: 58
Autores: Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado.
Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016

Optimization of flask culture medium and conditions for hyaluronic acid production by a streptococcus equisimilis mutant nc2168
Provedor de dados: 58
Autores: Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang.
A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040

Quality control for microbiological culture media: Is it enough to follow the NCCLS M22-A2 procedures?
Provedor de dados: 58
Autores: Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza; Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas.
Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality control; Culture medium; NCCLS.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003

Use of photocatalytic reduction to hasten preparation of culture media for saccharolytic Clostridium species
Provedor de dados: 58
Autores: Fukushima,Romualdo S.; Weimer,Paul J.; Kunz,Daniel A.
Cysteine is the preferred reducing agent used in the preparation of culture media for the growth of many strictly anaerobic microorganisms; however, redox potential reduction of cysteine is very slow, making it inconvenient if the medium is needed immediately or in large quantity. The time required to reduce culture medium containing resazurin (an indicator of reducing conditions) was dramatically shortened when the medium, after being injected with the reducing agent cysteine, was irradiated with incandescent light from a halogen lamp. Light intensity had an effect upon reduction time: tubes kept in the dark took more than 12 h to achieve the desired degree of anaerobiosis (measured spectrophotometrically by the bleaching of the indicator, resazurin)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaerobiosis; Culture medium; Cysteine; Light; Resazurin.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100006

Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media
Provedor de dados: 58
Autores: Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista; Raw,Isaías.
Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007

Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL)
Provedor de dados: 58
Autores: Silva,Carlos Henrique Pessôa de Menezes e.
The new ß-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum ß-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Culture medium; ESBL.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400006

Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of Mycobacterium bovis BCG
Provedor de dados: 58
Autores: Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina; Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías.
The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013

Chlorophyll a fluorescence of sweet potato plants cultivated in vitro and during ex vitro acclimatization
Provedor de dados: 61
Autores: Cassana,Francine F.; Falqueto,Antelmo R.; Braga,Eugenia J. B.; Peters,Jose A.; Bacarin,Marcos A.
Sweet potato (Ipomoea batatas L.) plants were cultivated in vitro in Murashige and Skoog (MS) medium with 20 and 40 g L-1 of sucrose under two different photon flux densities (21 and 60 µmol m-2 s-1). Leaves developed in vitro mostly showed high variable to maximum fluorescence ratio (F V/F M) , indicating good development of photosynthetic apparatus. This ratio decreased during leaf aging, especially in the plants cultivated in vitro on medium with higher sucrose concentration and higher photon flux. Leaves developed ex vitro showed high F V/F M ratio during whole experiment. The effective photochemical efficiency (F V'/F M') was maximum at 15th day after emergence of leaves. Photosynthetic potential rate was higher in leaves developed in vitro than in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Culture medium; Photon flux density; Photosystem; Sucrose.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300003

Effect of coconut water on growth of olive embryos cultured in vitro
Provedor de dados: 65
Autores: Souza,Rafaeli Aparecida Vieira de; Braga,Francyane Tavares; Setotaw,Tesfahun Alemu; Vieira Neto,João; Azevedo,Patrícia Helena de; Azevedo,Virgínia Helena de; Cançado,Geraldo Magela de Almeida.
The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea; Culture medium; Complex mixture; Tissue culture; Zygotic embryos.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000200016

European apple canker: morphophysiological variability and pathogenicity in isolates of Neonectria ditissima in southern Brazil
Provedor de dados: 65
Autores: Campos,Jonatas da Silva; Bogo,Amauri; Sanhueza,Rosa Maria Valdebenito; Casa,Ricardo Trezzi; Silva,Fabio Nascimento da; Cunha,Isabel Cristina da; Kuhnem Júnior,Paulo Roberto.
ABSTRACT:  European apple canker (EC) is caused by Neonectria ditissima, a pathogen officially registered as a quarantine pest in 2012. Thirty-five isolates of N. ditissima of different geographical regions of southern Brazil from apple branches showing symptoms of EC were identified by the specific pair primers Ch1 and Ch2 and analyzed concerning the virulence on Gala apple cultivar and morphophysiological characteristics. The disease symptoms were characterized and the isolates compared based on average mycelium growth (AMG), mycelium growth index (MGI), colony color, conidia type, dimensions and growth on potato dextrose agar (PDA), malt agar (AM), and synthetic SNAY (SN) culture media. Nineteen isolates showed the greatest AMG on PDA, forming three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus domestica L.; Culture medium; Mycelial and conidial morphophysiology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500152

Calli induction in leaf explants of coffee elite genotypes
Provedor de dados: 65
Autores: Rezende,Juliana Costa de; Carvalho,Carlos Henrique Siqueira de; Pasqual,Moacir; Santos,Ana Carolina Ramia; Carvalho,Stephan Malfitano de.
Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL &amp;amp; BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20µM) and 2-iP (2.5 e 20µM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica; Culture medium; Growth regulators.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000300004