Consulta: subjectFacets:"Cytochalasin B"
Registros recuperados: 6
Data/hora: 10/06/2024 10:13:48

A complementary method for production of tetraploid Crassostrea gigas using crosses between diploids and tetraploids with cytochalasin B treatments
Provedor de dados: 5
Autores: Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre.
We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,...
Tipo: Text
Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf

Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Ledu, Christophe; Mccombie, Helen.
The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention of polar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassostrea gigas embryos with sequential samples stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatment. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus...
Tipo: Text
Palavras-chave: Crassostrea gigas; Fertilization; Cytochalasin B; Polar body; Polyploidy.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2845.pdf

Polyploidy in the Manila clam Ruditapes philippinarum - 1 - Chemical induction and larval performances of triploids
Provedor de dados: 5
Autores: Dufy, Christian; Diter, Alain.
An effective treatment using catochalasin B (1 mg CB/ml dimethylsulfoxyde in 1 litre of sea water) was developed to induce triploidy in the Manila clam Ruditapes philippinurum (Adams and Reeve). The percentage of induced triploidy was assessed by karyological examination of embryos and spat. The treatment was found to be optimal between 20 to 35 minutes post-insemination exposure to CB, at 25'C, and produced 75.8 ± 5.7% triploid embryos on the average over four experiments. Growth and survival of larvae to metamorphosis were recorded for two different rearings each split into two replicates. Both larval rearings showed similar survival up to settlement: 19.3 and 27.0%, on average over two replicates, in the treated batches relative to the controls. Mean...
Tipo: Text
Palavras-chave: Larval survival; Larval growth; Bivalves; Cytochalasin B; Triploidy; Sruvie larvaire; Croissance larvaire; Bivalve; Cytochalasine B; Triploïdie.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/1990/publication-2752.pdf

Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
Provedor de dados: 47
Autores: Bevacqua,Romina J.; Fernandez-Martin,Rafael; Salamone,Daniel F.
Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meiotic maturation; Microfilaments; Cytochalasin B; Parthenogenesis.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100001

Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B
Provedor de dados: 62
Autores: Mezzalira,Alceu; Viera,Arnaldo Diniz; Barbieri,Dilmar Paulo; Machado,Mariana Fernandes; Thaler Neto,André; Bernadi,Mari Lourdes; Silva,Carlos Antônio Mondino; Rubin,Mara Iolanda Batistella.
In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Cytochalasin B; Embryos.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500008

Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development
Provedor de dados: 56
Autores: Feng,D.Q.; Zhou,Y.; Ling,B.; Gao,T.; Shi,Y.Y.; Wei,H.M.; Tian,Z.G.
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Oocyte activation; Parthenogenesis; Calcium; Cytochalasin B.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600006