Consulta: subjectFacets:"DNA barcoding"
Registros recuperados: 30
Data/hora: 06/06/2024 06:43:54

Response of a kleptoplastidic foraminifer to heterotrophic starvation: photosynthesis and lipid droplet biogenesis
Provedor de dados: 5
Autores: Jauffrais, Thierry; Lekieffre, Charlotte; Schweizer, Magali; Jesus, Bruno; Metzger, Edouard; Geslin, Emmanuelle.
The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to...
Tipo: Text
Palavras-chave: Foraminifera; Electron microscopy; DNA barcoding; Kleptoplasty; Lipids.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59894/63074.pdf

Biodiversity inventory of the grey mullets (Actinopterygii: Mugilidae) of the Indo‐Australian Archipelago through the iterative use of DNA‐based species delimitation and specimen assignment methods
Provedor de dados: 5
Autores: Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas.
DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications...
Tipo: Text
Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf

Current Methods for Seafood Authenticity Testing in Europe: is there a Need for Harmonisation?
Provedor de dados: 5
Autores: Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano.
Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing...
Tipo: Text
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf

Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
Provedor de dados: 5
Autores: Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: Text
Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf

The largest type study of Agaricales species to date: bringing identification and nomenclature of Phlegmacium (Cortinarius) into the DNA era
Provedor de dados: 16
Autores: Liimatainen, K.; Niskanen, T.; Dima, B.; Kytövuori, I.; Ammirati, J.F.; Frøslev, T.G.
Cortinarius is a species-rich and morphologically challenging genus with a cosmopolitan distribution. Many names have not been used consistently and in some instances the same species has been described two or more times under separate names. This study focuses on subg. Phlegmacium as traditionally deﬁned and includes species from boreal and temperate areas of the northern hemisphere. Our goals for this project were to: i) study type material to determine which species already have been described; ii) stabilize the use of Friesian and other older names by choosing a neo- or epitype; iii) describe new species that were discovered during the process of studying specimens; and iv) establish an accurate ITS barcoding database for Phlegmacium species. A total...
Tipo: Article / Letter to the editor
Palavras-chave: Basidiomycota; Diversity; DNA barcoding; ITS; Taxonomy; Typiﬁcation.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/532330

The genera of Hyphomycetes – 2011 update
Provedor de dados: 16
Autores: Seifert, K.A.; Gams, W.
This supplement to the taxonomic monograph The Genera of Hyphomycetes summarises information on 23 accepted new genera and c. 160 species described in 2011. These include three dematiaceous genera (Funbolia, Noosia, Pyrigemmula, all related to Dothideomycetes), a bulbil-producing genus, Spiroplana (Pleosporales), and two endophytic genera, the sterile Periglandula (Clavicipitaceae), and the hyaline, sympodial Micronematobotrys (Pyronemataceae). Slow-growing, morphologically-reduced, darkly pigmented fungi continue to be the source of new taxa, including the new genus Atramixtia (Dothioraceae). Eight new genera of darkly pigmented chlamydosporelike anamorphs were described from marine or subtidal environments (Glomerulispora, Halozoön, Hiogispora,...
Tipo: Article / Letter to the editor
Palavras-chave: Anamorphic fungi; DNA barcoding; Moulds; New genera; New species.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/532291

Comparative tadpole morphology in three species of frogs of the genus Spinomantis (Amphibia: Mantellidae)
Provedor de dados: 16
Autores: Vejarano, S.; Thomas, M.; Vences, M.
We describe the tadpoles of three species of Malagasy frogs, classified in the genus Spinomantis, based on specimens identified by DNA barcoding. The tadpole of Spinomantis aglavei is a typical Orton type IV larva. The oral disc is not emarginated laterally, but has two mid-ventral folds and a labial tooth row formula of 3(2- 3)/2(1). The tadpole of S. phantasticus is similar, the oral disc being laterally emarginated and having one medial fold; LTRF is 3/3(1). A third species, S. cf. fimbriatus is also similar to the other two species; the oral disc is not laterally emarginated but has three medial folds and LTRF is 3(2-3)/3(1). One shared character is a median fold or emargination in the lower part of the oral disc, although the shape of this fold is...
Tipo: Article / Letter to the editor
Palavras-chave: Anura; Spinomantis aglavei; Spinomantis phantasticus; Spinomantis fi mbriatus; Madagascar; Systematics; DNA barcoding; 42.82.
Ano: 2006
URL: http://www.repository.naturalis.nl/record/210775

Diversity, external morphology and ‘reverse taxonomy’ in the specialized tadpoles of Malagasy river bank frogs of the subgenus Ochthomantis (genus Mantidactylus)
Provedor de dados: 16
Autores: Randrianiaina, R.-D.; Strauß, A.; Glos, J.; Glaw, F.; Vences, M.
We provide detailed morphological descriptions of the tadpoles of Malagasy river bank frogs of the subgenera Ochthomantis and Maitsomantis (genus Mantidactylus, family Mantellidae), and data on relative abundance and habitat preferences of Ochthomantis species from Ranomafana National Park in southeastern Madagascar. Our study includes the tadpoles of six described and four undescribed candidate species. Eight of these larvae were previously unknown. Tadpoles were identified by DNA barcoding. Due to the very rudimentary taxonomic knowledge on Ochthomantis, we followed a ‘reverse taxonomy’ approach in which adult classification was to a great part determined on the basis of larval differences. By this procedure we even identified one candidate species whose...
Tipo: Article / Letter to the editor
Palavras-chave: Amphibia; Mantellidae; Madagascar; Tadpole morphology; DNA barcoding; 42.82.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/369710

Multi-gene phylogenetic analyses reveal species limits, phylogeographic patterns, and evolutionary histories of key morphological traits in Entoloma (Agaricales, Basidiomycota)
Provedor de dados: 16
Autores: Morgado, L.N.; Noordeloos, M.E.; Lamoureux, Y.; Gem, J.
Species from Entoloma subg. Entoloma are commonly recorded from both the Northern and Southern Hemispheres and, according to literature, most of them have at least Nearctic-Palearctic distributions. However, these records are based on morphological analysis, and studies relating morphology, molecular data and geographical distribution have not been reported. In this study, we used phylogenetic species recognition criteria through gene genealogical concordance (based on nuclear ITS, LSU, rpb2 and mitochondrial SSU) to answer specific questions considering species limits in Entoloma subg. Entoloma and their geographic distribution in Europe, North America and Australasia. The studied morphotaxa belong to sect. Entoloma, namely species like the notorious...
Tipo: Article / Letter to the editor
Palavras-chave: Biodiversity; DNA barcoding; Entolomataceae; Fungal biogeography; Molecular systematics; Phylogenetic species.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/532443

DNA barcoding resolves species complexes in Stigmella salicis and S. aurella species groups and shows additional cryptic speciation in S. salicis (Lepidoptera: Nepticulidae)
Provedor de dados: 16
Autores: Nieukerken, E.J. van; Mutanen, M.; Doorenweerd, C.
We sequenced the mitochondrial barcoding marker COI and nuclear marker EF1-alpha for most Nordic and other European species of the Stigmella salicis and S. aurella species groups. In the S. salicis group both markers confirm the synonymy of S. lappovimella with S. zelleriella. Specimens previously identified as Stigmella salicis and S. vimineticola are shown to form a complex of several cryptic species for which the taxonomy needs to be worked out. The species previously recorded as S. vimineticola from Norway represents probably an unnamed species. In the S. aurella group, the oligophagous Rosaceae feeders S. aurella and S. poterii are confirmed to be each a single oligophagous species. The synonymy between Stigmella ulmariae from Filipendula ulmaria and...
Tipo: Article / Letter to the editor
Palavras-chave: DNA barcoding; Phylogeny; Lepidoptera; 42.75.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/417740

DNA barcoding in Mucorales: an inventory of biodiversity
Provedor de dados: 16
Autores: Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de.
The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and...
Tipo: Article / Letter to the editor
Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/532353

Revision of the West Palaearctic Polistes Latreille, with the descriptions of two species: an integrative approach using morphology and DNA barcodes (Hymenoptera, Vespidae)
Provedor de dados: 16
Autores: Schmid-Egger, C.; Achterberg, K. van; Neumeyer, R.; Morinière, J.; Schmidt, S.
The genus Polistes is revised for the West Palaearctic region based on morphology and DNA barcodes. The revision includes all known West Palaearctic species, raising the number of species in Europe to 14 and to 17 for the West Palaearctic realm. DNA barcodes were recovered from 15 species, 14 of which belong to the subgenus Polistes, and one, P. wattii, to the subgenus Gyrostoma. An integrative taxonomic approach combining morphology and molecular data (DNA barcoding) was employed to resolve longstanding taxonomic problems in this group. Two species, P. austroccidentalis van Achterberg &amp; Neumeyer, sp. n. (= P. semenowi auctt.) from W and SW Europe and P. maroccanus Schmid-Egger, sp. n. from Morocco are described as new. Polistes bucharensis Erichson,...
Tipo: Article / Letter to the editor
Palavras-chave: Taxonomic revision; DNA barcoding; Key to species; Integrative taxon.
Ano: 2017
URL: http://www.repository.naturalis.nl/record/644038

Markets, mixtures and molecular methods: investigating medicinal plant and edible orchid diversity in Tanzania and Zambia
Provedor de dados: 16
Autores: Veldman, S.
Medicinal plants are an important source of primary healthcare for many people in Tanzania. These medicinal plants are harvested from the wild, and increasing commercial trade poses a serious threat to local plant populations. Currently it is unknown which species are traded and in what amounts. Across the southwestern border in Zambia, the traditional dish chikanda has transformed from a niche product to being a mainstream delicacy. One of the main ingredients are wild-harvested orchids, and these have become depleted throughout the country as an effect of the increased trade. It is unclear which orchid species are targeted and might be at risk of overharvesting. The aims of my doctorate are to map harvest and trade of Tanzanian medicinal plants and...
Tipo: Book (monograph)
Palavras-chave: DNA barcoding; Africa; Species delimitation; Orchids; Medicinal plants.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/668031

A new species of Fizesereneia Takeda &amp; Tamura, 1980 (Crustacea: Brachyura: Cryptochiridae) from the Red Sea and Oman
Provedor de dados: 16
Autores: Meij, S.E.T. van der; Berumen, M.L.; Paulay, G.
A new species of cryptochirid crab, Fizesereneia panda van der Meij, is described and illustrated based on specimens collected from the scleractinian corals Lobophyllia cf. hemprichii and L. cf. corymbosa from the Farasan Banks, Farasan Islands, and the reefs off Thuwal in the Saudi Arabian Red Sea, and from Symphyllia recta from reefs in the Gulf of Oman. This is the second cryptochirid species with the Red Sea as type locality. It can be separated from its congeners by the subrectangular carapace, raised midline and the complete division of the carapace depressions, and reddish black colour pattern of these concavities in live specimens. This new species is the seventh assigned to Fizesereneia. A DNA barcode for the new species has been deposited in...
Tipo: Article / Letter to the editor
Palavras-chave: DNA barcoding; Gall crabs; Host specificity; Lobophylliidae; Saudi Arabia; Scleractinia; Taxonomy; 42.74.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/533931

Evidence for the validity of Protatlanta sculpta (Gastropoda: Pterotracheoidea)
Provedor de dados: 16
Autores: Wall-Palmer, D.; Burridge, A.K.; Peijnenburg, K.C.T.A.; Janssen, A.; Goetze, E.; Kirby, R.; Hart, M.B.; Smart, C.W.
The genus Protatlanta is thought to be monotypic and is part of the Atlantidae, a family of shelled heteropods. These microscopic planktonic gastropods are poorly known, although research on their ecology is now increasing in response to concerns about the effects of ocean acidification on calcareous plankton. A correctly implemented taxonomy of the Atlantidae is fundamental to this progressing field of research and it requires much attention, particularly using integrated molecular and morphological techniques. Here we use DNA barcoding, shell morphology and biogeography to show that the genus Protatlanta includes at least two valid species in the Atlantic Ocean. Protatlanta souleyeti and Protatlanta sculpta were found to be separate species, with...
Tipo: Article / Letter to the editor
Palavras-chave: Atlantic Ocean; Biogeography; DNA barcoding; Morphometrics; Protatlanta; Shelled heteropod.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/619150

Evolutionary history of the sequestrate genus Rossbeevera (Boletaceae) reveals a new genus Turmalinea and highlights the utility of ITS minisatellite-like insertions for molecular identification
Provedor de dados: 16
Autores: Orihara, T.; Lebel, T.; Ge, Z.-W.; Smith, M.E.; Maekawa, N.
The sequestrate (truffle-like) basidiomycete genera Rossbeevera, Chamonixia, and Octaviania are closely related to the epigeous mushroom genera Leccinum and Leccinellum. In order to elucidate the properties and placement of several undescribed sequestrate taxa in the group and to reveal the evolutionary history of Rossbeevera and its allies, we conducted phylogenetic analyses based on three nuclear (ITS, nLSU, EF-1α) and two mitochondrial DNA loci (ATP6 and mtSSU) as well as precise morphological observations. Phylogenetic analyses of three nuclear loci suggest a complex evolutionary history with sequestrate fruiting bodies present in several clades, including a previously unrecognized sister clade to Rossbeevera. Here we propose a new sequestrate genus,...
Tipo: Article / Letter to the editor
Palavras-chave: Biogeography; Cryptic species; DNA barcoding; Hypogeous fungi; Introgression; Species tree.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/611857

Species-level para- and polyphyly in DNA barcode gene trees: strong operational bias in European Lepidoptera
Provedor de dados: 16
Autores: Mutanen, M.; Kivelä, S.M.; Vos, R.A.; Doorenweerd, C.; Ratnasingham, S.; Hausmann, A.; Huemer, P.; Dinca, V.; Nieukerken, E.J. van; Lopez-Vaamonde, C.; Vila, R.; Aarvik, L.; Decaëns, T.; Efetov, K.A.; Hebert, P.D.N.; Johnsen, A.; Karsholt, O.; Pentinsaari, M.; Rougerie, R.; Segerer, A.; Tarmann, G.; Zahiri, R.; Godfray, H.C.J.
The proliferation of DNA data is revolutionizing all fields of systematic research. DNA barcode sequences, now available for millions of specimens and several hundred thousand species, are increasingly used in algorithmic species delimitations. This is complicated by occasional incongruences between species and gene genealogies, as indicated by situations where conspecific individuals do not form a monophyletic cluster in a gene tree. In two previous reviews, non-monophyly has been reported as being common in mitochondrial DNA gene trees. We developed a novel web service “Monophylizer” to detect non-monophyly in phylogenetic trees and used it to ascertain the incidence of species non-monophyly in COI (a.k.a. cox1) barcode sequence data from 4977 species...
Tipo: Article / Letter to the editor
Palavras-chave: DNA barcoding; Gene tree; Lepidoptera; Mitochondrial COI; Mitochondrial cox1; Paraphyly; Polyphyly; Species delimitation; Species monophyly.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/648758

The enigmatic Marmorkrebs (marbled crayfish) is the parthenogenetic form of Procambarus fallax (Hagen, 1870)
Provedor de dados: 16
Autores: Martin, P.; Dorn, N.J.; Kawai, T.; Heiden, C. van der; Scholtz, G.
A mysterious parthenogenetic cambarid crayfish (the Marmorkrebs) has been spreading across the globe for the past decade. We compare this crayfish directly to two other cambarids, Procambarus fallax and P. alleni, that have been suggested to be related or even identical to the Marmorkrebs. Using external morphology and sequences of two mitochondrial genes we show clear correspondences between Marmorkrebs and P. fallax, a species found natively throughout peninsular Florida, USA. Based on these congruent results we suggest that the Marmorkrebs is the parthenogenetic form of P. fallax. This finding has potential evolutionary and ecological implications at several levels. The Marmorkrebs might be a type of geographical parthenogenesis, but a natural...
Tipo: Article / Letter to the editor
Palavras-chave: 12S rRNA; Annulus ventralis; COI; DNA barcoding; Species concept; Thelytoky; 42.74.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/355212

Morphological difference between upperside and underside leaf-mining larvae of Phyllocnistis Unipunctella (Stephens, 1834) (Lep. Gracillariidae) and its changing phenology
Provedor de dados: 16
Autores: Jordan, M.P.; Langmaid, J.R.; Doorenweerd, C.
Larvae of Phyllocnistis unipunctella (Stephens) mining the upperside and underside of leaves of Populus spp. were compared in terms of gross morphology and the COI DNA barcoding section of mitochondrial DNA. It was discovered that larvae feeding on the underside did not show the dark pigmentation of the prothoracic plate as described in the literature. Larvae were found feeding two months earlier than normal and at least one extra generation was observed. More work isrequired to determine the possibility ofspeciation.
Tipo: Article / Letter to the editor
Palavras-chave: Lepidoptera; Prothoracic plate; DNA barcoding; Phenology.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/610600

One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes
Provedor de dados: 16
Autores: Stielow, J.B.; Lévesque, C.A.; Seifert, K.A.; Meyer, W.; Irinyi, L.; Smits, D.; Renfurm, R.; Verkley, G.J.M.; Groenewald, M.; Chaduli, D.; Lomascolo, A.; Welti, S.; Lesage-Meessen, L.; Favel, A.; Al-Hatmi, A.M.S.; Damm, U.; Yilmaz, N.; Houbraken, J.; Lombard, L.; Quaedvlieg, W.; Binder, M.; Vaas, L.A.I.; Vu, D.; Yurkov, A.; Begerow, D.; Roehl, O.; Guerreiro, M.; Fonseca, A.; Samerpitak, K.; Diepeningen, A.D. van; Dolatabadi, S.; Moreno, L.F.; Casaregola, S.; Mallet, S.; Jacques, N.; Roscini, L.; Egidi, E.; Bizet, C.; Garcia-Hermoso, D.; Martin, M.P.; Deng, S.; Groenewald, J.Z.; Boekhout, T.; Beer, Z.W. de; Barnes, I.; Duong, T.A.; Wingfield, M.J.; Hoog, G.S. de; Crous, P.W.; Lewis, C.T.; Hambleton, S.; Moussa, T.A.A.; Al-Zahrani, H.S.; Almaghrabi, O.A.; Louis-Seize, G.; Assabgui, R.; McCormick, W.; Omer, G.; Dukik, K.; Cardinali, G.; Eberhardt, U.; Vries, M. de; Robert, V.
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Ampliﬁcation efﬁciencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across &gt; 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α);...
Tipo: Article / Letter to the editor
Palavras-chave: DNA barcoding; ITS supplement; Molecular taxonomy; Phylogeny; Species identiﬁcation; Universal primers.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/588731

DNA barcodes for soil animal taxonomy
Provedor de dados: 86
Autores: Rougerie,Rodolphe; Decaëns,Thibaud; Deharveng,Louis; Porco,David; James,Sam W.; Chang,Chih-Han; Richard,Benoit; Potapov,Mikhail; Suhardjono,Yayuk; Hebert,Paul D.N.
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collembola; Cryptic diversity; DNA barcoding; Oligochaeta; Species identification; Taxonomic crisis; Taxonomic impediment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800002

First record of the sedge feeder Bactra verutana Zeller (Lepidoptera: Tortricidae) in Chile based on morphology and DNA barcodes
Provedor de dados: 92
Autores: Vargas,Héctor A.; Vargas-Ortiz,Marcelo.
Abstract The sedge-feeding moth Bactra verutana Zeller, 1875 (Lepidoptera: Tortricidae: Olethreutinae: Bactrini), described from Dallas, Texas, USA, is widespread, recorded throughout much North America, Central and South America, including the Caribbean, and Africa. The species is recorded for the first time from Chile based on specimens collected in the coastal valleys of the Atacama Desert, where its larvae feed on Cyperus corymbosus Rottb. var. subnodosus (Nees &amp; Meyen) Kük. (Cyperaceae). A single DNA barcode haplotype, which is widespread in USA, was found in two Chilean specimens sequenced.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Atacama Desert; Cyperaceae; Cyperus corymbosus; DNA barcoding.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200104

Two new species of Mycodrosophila (Diptera, Drosophilidae) proposed by molecular and morphological approaches, with a key to American species
Provedor de dados: 92
Autores: Junges,João; Gottschalk,Marco Silva; Loreto,Elgion Lucio da Silva; Robe,Lizandra Jaqueline.
ABSTRACT There are approximately 130 species of MycodrosophilaOldenberg, 1914 worldwide, although only nine species were recorded in American countries so far, three of which are exclusively Nearctic, five exclusively Neotropical and one found in both biogeographic regions (Mycodrosophila projectans). Such a small number of American species is likely a consequence of collecting bias, which favors the capture of frugivorous drosophilids, and to the general absence of Neotropical Mycodrosophila studies in the last 50 years. Here, we describe two commonly sampled species of Mycodrosophila from the Amazonian and Pampa Brazilian biomes, which share morphological similarities with Mycodrosophila neoprojectans and M. projectans, respectively. We compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian forest; Cytochrome oxidase subunit I; DNA barcoding; Mycophagous drosophilids; Pampa biome.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000100030

Saving our science from ourselves: the plight of biological classification
Provedor de dados: 92
Autores: Ebach,Malte C.; Carvalho,Marcelo R. de; Nihei,Silvio S.
Saving our science from ourselves: the plight of biological classification. Biological classification ( nomenclature, taxonomy, and systematics) is being sold short. The desire for new technologies, faster and cheaper taxonomic descriptions, identifications, and revisions is symptomatic of a lack of appreciation and understanding of classification. The problem of gadget-driven science, a lack of best practice and the inability to accept classification as a descriptive and empirical science are discussed. The worst cases scenario is a future in which classifications are purely artificial and uninformative.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Classification; DNA barcoding; Morphology; Nomenclature; Systematic; Taxonomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000200001

External morphology of immature stages of Zaretis strigosus (Gmelin) and Siderone galanthis catarina Dottax and Pierre comb. nov., with taxonomic notes on Siderone (Lepidoptera: Nymphalidae: Charaxinae)
Provedor de dados: 92
Autores: Dias,Fernando Maia Silva; Oliveira-Neto,José Francisco de; Casagrande,Mirna Martins; Mielke,Olaf Hermann Hendrik.
ABSTRACT The external morphology of immature stages of Zaretis strigosus (Gmelin, [1790]) and Siderone galanthis catarina Dottax and Pierre, 2009 comb. nov. from southern Brazil are described. Additionally, morphology of the adults and sequences of the mitochondrial gene cytochrome oxidase, subunit I, were analyzed in order to evaluate the taxonomy of Siderone galanthis Hübner, [1823]. Immatures were collected on Casearia sylvestris (Salicaceae) in Curitiba, Paraná, and Balneário Barra do Sul, Santa Catarina, Brazil, and reared at the laboratory. Morphological descriptions and illustrations are provided, based on observations through stereoscopic and optic microscopes attached to camera lucida; results are compared and discussed and immature stages of some...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaeini; Chaetotaxy; DNA barcoding; Leafwing; Zaretidinae.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262015000400307

Phyllocnistis hemera sp. nov. (Lepidoptera: Gracillariidae): a new species of leaf-miner associated with Daphnopsis fasciculata (Thymelaeaceae) in the Atlantic Forest
Provedor de dados: 92
Autores: Fochezato,Júlia; Brito,Rosângela; Isaias,Rosy Mary dos Santos; Gonçalves,Gislene Lopes; Moreira,Gilson R.P.
ABSTRACT During recent studies performed in the Atlantic Forest, a new species of Phyllocnistinae (Gracillariidae), Phyllocnistis hemera sp. nov., leaf miner of Daphnopsis fasciculata (Thymelaeaceae) was discovered. The adults are described and illustrated as well as the immature stages, with notes on natural history including a description of the leaf mine. Additionally, DNA barcode sequences were compared to other representatives of Phyllocnistinae to test for the specific status of P. hemera and to infer phylogenetic relationships. This is the fifth species described for the genus Phyllocnistis in the Atlantic Forest and the first record of a gracillarid mining Thymelaeaceae leaves.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microlepidoptera; New species; Morphology; DNA barcoding; Neotropical region.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100057

DNA barcoding: a molecular appliance for fish species identification support
Provedor de dados: 22
Autores: Tresbach, Rafael Hencke; Cerqueira, Natalia Menezes; Medeiros, Sarah Ramos; Gutierrez, Harold Julián Pérez; Hernández, Natalia Ossa; Rodrigues, Marília Danyelle Nunes.
The identification of genus and species is made by systematic, by noticing morphological and anatomical characteres which were the phenotype expression. However, changes in one or more gene bases can result in phenotypes similarities between different species, inducing an identification error. Alternatively, molecular appliances based in mithocondrial and ribosomal genes appeared. This study aims to compile information about the molecular identification and present the South American Catfish (Rhamdia quelen) case, for which there is controversy generated by taxonomy identification. The different genes used to identification have their particularities, like insertions and deletions on the case of 16S rRNA and the 18S rRNA. Besides these ribosomal genes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA barcoding; Peixes; Rhamdia quelen; COI; DNA mitocondrial; DNA ribossomal.
Ano: 2015
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/10408

The Tonkin weakfish, Cynoscion similis (Sciaenidae, Perciformes), an endemic species of the Amazonas-Orinoco Plume
Provedor de dados: 36
Autores: CAIRES,Rodrigo Antunes; SANTOS,Wagner. C. R. dos; MACHADO,Leonardo; OLIVEIRA,Claudio; CERQUEIRA,Najila N. C. D.; ROTUNDO,Matheus Marcos; OLIVEIRA,Cintia; MARCENIUK,Alexandre P.
ABSTRACT Despite its importance in biogeographical, ecological, and commercial terms, the fish fauna of the northern Brazilian coast is still poorly known, representing the least sampled portion of the Brazilian Exclusive Economic Zone. We collected Tonkin weakfish, Cynoscion similis specimens during extensive surveys of the northern Brazilian coast and concluded that C. similis is common in this region. While the species had not previously been reported for the northern Brazilian state of Pará, it may have been recorded in studies of industrial fisheries, being identified only as Cynoscion sp. or by the common name pescada negra. This reinforces the need for the reliable taxonomical identification of species, to guarantee the collection of accurate data...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian north coast; Cynoscion jamaicensis; Industrial fisheries; DNA barcoding; Reliable taxonomical identification.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000300197

Pharmacognostical study of Desmodium caudatum
Provedor de dados: 42
Autores: LI,JUNNI; LIN,XIAOJING; TANG,GENGQIU; LI,RONG; WANG,DONG; JI,SHENGGUO.
Abstract: De﻿﻿smodium caudatum (Thunb.) DC, is an ever-green plant widely used in the central and southern China with great economic value for their medical values on fever, dysentery, gastroenteritis, rectal prolapse, snake bites, mastitis, and boils carbuncle. Despite its extensive uses as a traditional Chinese medicine, no systematic research on the identification of Desmodium caudatum ha﻿s been reported. In﻿ this study, tr﻿﻿aditional ph﻿﻿﻿armacognostical identification including th﻿e bo﻿﻿﻿﻿tanical origin and morphological characters, medicinal material characters, microscopic characters, physicochemical parameters determination and phytochemical screening, and DNA barcoding analysis were employed to establish an accurate and effective identification...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Desmodium caudatum; DNA barcoding; Quality standardization; Traditional pharmacognostical identification.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300709

Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
Provedor de dados: 65
Autores: Barbosa,Marcílio Souza; Barbosa,Suzanne Nunes; Nagamoto,Nilson Satoru; Forti,Luiz Carlos; lmeida,Cícero.
ABSTRACT: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; DNA barcoding; Leaf-cutter; Ants.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500150