Consulta: subjectFacets:"DNA vaccine"
Registros recuperados: 12
Data/hora: 11/06/2024 10:13:53

DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice
Provedor de dados: 19
Autores: Takashima, Yasuhiro; Xuan, Xuenan; Shirafuji, Hiroaki; Zhang, Guohong; Otsuka, Haruki.
To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility  of the plasmid pCX-p23Fc as a DNA vaccine...
Palavras-chave: C. parvum; DNA vaccine; Fc; Interferon-8.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/147

Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein
Provedor de dados: 45
Autores: Cândido,A.L.; Resende,M.; Bessa,L.R.G.; Leite,R.C.
A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine herpesvirus-1.2a; Glycoprotein D gene; DNA vaccine; ELISA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300002

The immunogenicity and anti-tumor effects of a lung cancer DNA vaccine harboring a MUC-1 and GM-CSF fusion gene
Provedor de dados: 52
Autores: Yang,Zhen; Jiang,DanDan; Zhu,Qiang; Xiao,BinBin; Chen,Liang An.
ABSTRACT DNA vaccines have been shown to be an effective approach to induce antigen-specific cellular and humoral immunity. However, the inability of DNA vaccines to elicit strong immune responses in clinical trials limits the application of DNA vaccines. Here, we developed a new DNA vaccine based on MUC1, which has been suggested as a potential target for lung cancer therapy, and we enhanced the potency of the DNA vaccine by including granulocyte-macrophage colony-stimulating factor (GM-CSF) as an adjuvant. A series of DNA plasmids encoding MUC1, human GM-CSF and their conjugates were constructed and injected into female mice intramuscularly (i.m.). This action was followed by an electric pulse. The humoral and cellular immune responses after immunization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MUC1; GM-CSF; Lung cancer; DNA vaccine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100331

Construction of a fusion plasmid containing the PSCA gene and cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and its anti-tumor effect in an animal model of prostate cancer
Provedor de dados: 56
Autores: Mai,T.J.; Ma,R.; Li,Z.; Bi,S.C.
Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a negative regulator of T cell activation, which competes with CD28 for B7.1/B7.2 binding, and which has a greater affinity. Fusion of specific antigens to extracellular domain of CTLA4 represents a promising approach to increase the immunogenicity of DNA vaccines. In this study, we evaluated this interesting approach for CTLA4 enhancement on prostate stem cell antigen (PSCA)-specific immune responses and its anti-tumor effects in a prostate cancer mouse model. Consequently, we constructed a DNA vaccine containing the PSCA and the CTLA-4 gene. Vaccination with the CTLA4-fused DNA not only induced a much higher level of anti-PSCA antibody, but also increased PSCA-specific T cell response in mice. To...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostate cancer; DNA vaccine; PSCA; CTLA-4.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100601

Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D
Provedor de dados: 56
Autores: Diniz,M.O.; Ferreira,L.C.S.
Anti-cancer DNA vaccines have attracted growing interest as a simple and non-invasive method for both the treatment and prevention of tumors induced by human papillomaviruses. Nonetheless, the low immunogenicity of parenterally administered vaccines, particularly regarding the activation of cytotoxic CD8+ T cell responses, suggests that further improvements in both vaccine composition and administration routes are still required. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Gene gun; DNA vaccine; HPV-16; Anti-cancer vaccine.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500007

Regulation of transgene expression in genetic immunization
Provedor de dados: 56
Autores: Harms,J.S.; Oliveira,S.C.; Splitter,G.A.
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/FONT&gt; on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; Gene therapy; Regulation; Transcription; Transgene.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003

New vaccine strategies against enterotoxigenic Escherichia coli: II: Enhanced systemic and secreted antibody responses against the CFA/I fimbriae by priming with DNA and boosting with a live recombinant Salmonella vaccine
Provedor de dados: 56
Autores: Lásaro,M.O.; Alves,A.M.B.; Guillobel,H.C.R.; Almeida,D.F.; Ferreira,L.C.S.
The induction of systemic (IgG) and mucosal (IgA) antibody responses against the colonization factor I antigen (CFA/I) of enterotoxigenic Escherichia coli (ETEC) was evaluated in mice primed with an intramuscularly delivered CFA/I-encoding DNA vaccine followed by two oral immunizations with a live recombinant Salmonella typhimurium vaccine strain expressing the ETEC antigen. The booster effect induced by the oral immunization was detected two weeks and one year after the administration of the DNA vaccine. The DNA-primed/Salmonella-boosted vaccination regime showed a synergistic effect on the induced CFA/I-specific systemic and secreted antibody levels which could not be attained by either immunization strategy alone. These results suggest that the combined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; ETEC; CFA/I fimbriae; Salmonella bivalent vaccine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200014

Antigen-presenting cells transfected with Hsp65 messenger RNA fail to treat experimental tuberculosis
Provedor de dados: 56
Autores: Rocha,C.D.; Trombone,A.P.F.; Lorenzi,J.C.C.; Almeida,L.P.; Gembre,A.F.; Padilha,E.; Ramos,S.G.; Silva,C.L.; Coelho-Castelo,A.A.M.
In the last several years, the use of dendritic cells has been studied as a therapeutic strategy against tumors. Dendritic cells can be pulsed with peptides or full-length protein, or they can be transfected with DNA or RNA. However, comparative studies suggest that transfecting dendritic cells with messenger RNA (mRNA) is superior to other antigen-loading techniques in generating immunocompetent dendritic cells. In the present study, we evaluated a new therapeutic strategy to fight tuberculosis using dendritic cells and macrophages transfected with Hsp65 mRNA. First, we demonstrated that antigen-presenting cells transfected with Hsp65 mRNA exhibit a higher level of expression of co-stimulatory molecules, suggesting that Hsp65 mRNA has immunostimulatory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Therapeutic immunization; CD; MHSP65; DNA vaccine; APCs.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200011

Effect of the Kozak sequence on seroconversion of mice immunized with a DNA vaccine against swine colibacilosis
Provedor de dados: 58
Autores: Michelon,André; Michelon,Marcelo; Simionatto,Simone; Lagranha,Valeska Lizzi; Conceição,Fabrício Rochedo; Vaz,Eliana Knackfus; Cerqueira,Gustavo Maia; Dellagostin,Odir Antonio.
The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P&lt;0.05) than the one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine colibacilosis; DNA vaccine; FaeC; Kozak sequence.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500029

Immune response to dna vaccine expressing transferrin binding protein a gene of Pasteurella multocida
Provedor de dados: 58
Autores: Singh,Satparkash; Singh,Vijendra Pal; Cheema,Pawanjit Singh; Sandey,Maninder; Ranjan,Rajeev; Gupta,Santosh Kumar; Sharma,Bhaskar.
Haemorrhagic Septicaemia (HS), an acute and fatal disease of cattle and buffalo is primarily caused by serotype B:2 or E:2 of Pasteurella multocida. The transferrin binding protein A (TbpA) has been found to act as immunogen and potent vaccine candidate in various Gram negative bacteria including P. multocida. The present study was carried out to evaluate the potential of this antigen as a DNA vaccine against HS in mice model. The tbpA gene of P. multocida serotype B:2 was cloned in a mammalian expression vector alone and along with murine IL2 gene as immunological adjuvant to produce monocistronic and bicistronic DNA vaccine constructs, respectively. The immune response to DNA vaccines was evaluated based on serum antibody titres and lymphocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA vaccine; Haemorrhagic septicaemia; IL2; Pasteurella multocida; Transferrin binding protein.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200043

Live bacterial vaccine vectors: An overview
Provedor de dados: 58
Autores: Silva,Adilson José da; Zangirolami,Teresa Cristina; Novo-Mansur,Maria Teresa Marques; Giordano,Roberto de Campos; Martins,Elizabeth Angélica Leme.
Genetically attenuated microorganisms, pathogens, and some commensal bacteria can be engineered to deliver recombinant heterologous antigens to stimulate the host immune system, while still offering good levels of safety. A key feature of these live vectors is their capacity to stimulate mucosal as well as humoral and/or cellular systemic immunity. This enables the use of different forms of vaccination to prevent pathogen colonization of mucosal tissues, the front door for many infectious agents. Furthermore, delivery of DNA vaccines and immune system stimulatory molecules, such as cytokines, can be achieved using these special carriers, whose adjuvant properties and, sometimes, invasive capacities enhance the immune response. More recently, the unique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial vector; Vaccine delivery system; DNA vaccine; Cancer vaccine; Antigen presentation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400001

Enhancing immune effects of a DNA vaccine against kidney cancer using CD40L as an adjuvant
Provedor de dados: 60
Autores: Jiang,Guohong; Song,Rixin; Ma,Peilong.
The use of specific combinations of antigens and adjuvant represents a promising approach for increasing the immunogenicity of DNA vaccines. In the present study, we evaluated the immunity and antitumor effects of DNA vaccines with G250 as the target antigen in a mouse model of renal cell carcinoma. We constructed two recombinant plasmids, pVAX1-G250 and pVAX1-CD40L. The recombinant plasmids were injected into mice by intramuscular injection and electrical pulse stimulation. ELISA and ELISPOT experiments were performed to evaluate the corresponding humoral and cellular immune responses following immunization. To further investigate the antitumor potential of the DNA vaccines, we established a tumor-bearing mouse model expressing G250 target antigen. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney cancer; G250; CD40L; DNA vaccine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100555