Consulta: subjectFacets:"Denervation"
Registros recuperados: 13
Data/hora: 02/06/2024 11:53:38

Apoptosis in Denervated Skeletal Muscle
Provedor de dados: 77
Autores: Davatz,Giovanna Castilho; Andreo,Jesus Carlos; Rodrigues,Antonio de Castro; Rosa Júnior,Geraldo Marco; Moraes,Luiz Henrique Rapucci.
The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated...
Tipo: Journal article
Palavras-chave: Skeletal muscle; Denervation; Apoptosis; Rat.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300009

Ultrastructural Study of the Denervated Diaphragm in Rats
Provedor de dados: 77
Autores: Torrejais,M. M; Soares,J. C; Matheus,S. M. M; Mello,J. M; Francia-Farje,L. A. D; Jeronimo,L. C; Vicente,E. J. D.
The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and...
Tipo: Journal article
Palavras-chave: Denervation; Diaphragm; Muscle fiber; Ultrastructural; Rat.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300025

Morphometric and Morphological Analysis of Neuromuscular Junction Alterations in the Denervated Rat Diaphragm
Provedor de dados: 77
Autores: Torrejais,M. M; Soares,J. C; Matheus,S. M. M; Mello,J. M; Francia-Farje,L. A. D; Vicente,E. J. D.
The morphological and structural alterations that occur in the neuromuscular junctions of the denervated rat diaphragm were studied. Fifteen adult male albino rats (Rattus norvegicus) aged about 60 days and with a mean weight of 200 g were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by section of the phrenic nerve and the right antimere was used as control. Each antimere was divided into three fragments: one was used for histochemical (nonspecific esterase) and morphometric study of neuromuscular junctions, and the other two were used for transmission and scanning electron microscopy (SEM) analysis. Histochemical analysis of...
Tipo: Journal article
Palavras-chave: Diaphragm; Neuromuscular junction; Denervation; Rat.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400043

Correlation between Innervation of Skeletal Muscles and Myoblast Stem Cells
Provedor de dados: 77
Autores: Wu,Jiang; Sun,Xiaojuan; Zhu,Min; Zhu,Chongtao; Chen,Bin; Xu,Yongqing; Ouyang,Jun; Liao,Hua; Qiu,Xiaozhong.
In order to explore the change rule of myoblast stem cells (satellite cells, SCs) in the denervated and re-innervated muscle and to investigate the cellular mechanism of the morphological and functional changes of the muscle, denervated muscle atrophy and nerve regeneration models were established in one-month-old rats. Postoperative indexes such as muscle wet weight, cell section areas, content of collagen fibers and DNA, electrophysiology, numbers of SCs in the triceps muscle of calf were dynamically tested. After denervation, the muscle wet weight and cell area reduced rapidly, and the collagen fiber content increased slowly. The number of SCs increased at first, and then declined suddenly two months later. From 4 to 5 weeks after re-neuralization,...
Tipo: Journal article
Palavras-chave: Satellite cells; Atrophy; Denervation; Innervations; Stem cells.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400043

Morphologic Alterations Resulting from Denervation of the Diaphragm in Rats
Provedor de dados: 77
Autores: Torrejais,M. M; Soares,J. C; Matheus,S. M. M; Francia-Farje,L. A. D; Mello,J. M; Vicente,E. J. D.
The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm...
Tipo: Journal article
Palavras-chave: Diaphragm; Muscle fiber; Denervation; Rat.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300061

Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
Provedor de dados: 42
Autores: NUNES-TAVARES,NILSON; CUNHA-E-SILVA,NARCISA LEAL; HASSÓN-VOLOCH,AÍDA.
Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Choline acetyltransferase; Electrocyte; Denervation; Immunohistochemistry.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007

Study of the myenteric and submucous plexuses after BAC treatment in the intestine of rats
Provedor de dados: 47
Autores: Buttow,Nilza Cristina; Santin,Miriam; Conci Macedo,Luciana; Neres Teixeira,Aline Cristina; Novakowski,Gisele Caroline; Bolonheis Armelin,Taíse Roberta; Assmann,Kathya.
A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI - ileum control; CBI - denervated ileum; SC - colon control; and CBC - denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2. The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric plexus there was also a significant decrease in the number of ganglia/mm2 (13.8% in group CBI and 52.14% in group CBC) and in the number of neurons/ganglion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myenteric plexus; Submucous plexus; Morphology; Benzalkonium chloride; Denervation.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000200002

Ciliary Neurotrophic Factor (CNTF) affects the excitable and contractile properties of innervated skeletal muscles
Provedor de dados: 48
Autores: RAMIREZ,BEATRIZ U; RETAMAL,LEIRA; VERGARA,CECILIA.
The well-established trophic role of CNTF upon neurons led to performing clinical trials in patients of neurodegenerative diseases. However, trials were suspended due to side effects such as severe weight loss, hyperalgesia, coughing, muscle cramps and pain. So far it is not known how CNTF triggers the problems related to skeletal muscle cramps and pain. CNTF has also been described as a myotrophic factor for denervated skeletal muscles, but the possibility that it affects innervated muscles has also been considered. Since a myotrophic factor could be a valuable tool for treatment of several muscle diseases, we studied the effects of low doses of CNTF delivered systemically by an osmotic pump, over the electrical and mechanical properties of innervated and...
Tipo: Journal article
Palavras-chave: CNTF; Contraction and relaxation time; Denervation; EMG; Fibrillation; Skeletal muscle.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300003

Glucose metabolism in rats submitted to skeletal muscle denervation
Provedor de dados: 52
Autores: Nunes,Wilton Marlindo Santana; Mello,Maria Alice Rostom de.
This study analyzed the local and systemic effects of immobilization by denervation of the skeletal muscle on glucose metabolism. The rats were submitted to section of the right paw sciatic nerve. A reduction was observed in glucose uptake by the isolated soleus muscle of the denervated paw after 3 and 7 days, but not after 28 days in relation to the control animals. There was no difference after 3 and 7 days in glucose uptake by the soleus muscle of the opposite intact paw in relation to the control. There was increased glucose uptake in the same paw 28 days after denervation. The rate of glucose removal in response to exogenous insulin after 28 days of denervation was significantly higher than in control animals and those observed after 3 and 7 days of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denervation; Glucose metabolism; Skeletal muscle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500006

Effect of salbutamol on innervated and denervated rat soleus muscle
Provedor de dados: 56
Autores: Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M.
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008

PPM1B and P-IKKβ expression levels correlated inversely with rat gastrocnemius atrophy after denervation
Provedor de dados: 56
Autores: Wei,Jian; Liang,Bing-Sheng.
Activated inhibitor of nuclear factor-κB kinase β (IKKβ) is necessary and sufficient for denervated skeletal muscle atrophy. Although several studies have shown that Mg2+/Mn2+-dependent protein phosphatase 1B (PPM1B) inactivated IKKβ, few studies have investigated the role of PPM1B in denervated skeletal muscle. In this study, we aim to explore the expression and significance of PPM1B and phosphorylated IKKβ (P-IKKβ) during atrophy of the denervated gastrocnemius. Thirty young adult female Wistar rats were subjected to right sciatic nerve transection and were sacrificed at 0 (control), 2, 7, 14, and 28 days after denervation surgery. The gastrocnemius was removed from both the denervated and the contralateral limb. The muscle wet weight ratio was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular atrophy; Denervation; PPM1B; P-IKKβ; IKKβ; RT-PCR; Western blot.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800004

Implantation of muscle satellite cells overexpressing myogenin improves denervated muscle atrophy in rats
Provedor de dados: 56
Autores: Shen,H.; Lv,Y.; Shen,X.Q.; Xu,J.H.; Lu,H.; Fu,L.C.; Duan,T.
This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1 week after surgical denervation. Controls included injections of untransfected MSCs or the vehicle only. Muscles were harvested and analyzed at 2, 4, and 24 weeks post-transplantation. Immunofluorescence confirmed MyoG overexpression in MTMs. The muscle wet weight ratio was significantly reduced at 2 weeks after MTM injection (67.17±6.79) compared with muscles injected with MSCs (58.83±5.31) or the vehicle (53.00±7.67; t=2.37, P=0.04 and t=3.39, P=0.007, respectively). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Denervation; Muscle atrophy; Satellite cells; Myogenin; Transplantation; Transfection.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200609

Effects of long-term fluoxetine treatment on adrenergic plasticity in rat vas deferens
Provedor de dados: 60
Autores: Souza,Cláudio Fontes; Garcez-do-Carmo,Lúcia; Conceição,Isaltino Marcelo; Freitas,Thalma Ariani; Frussa-Filho,Roberto.
Chronic antidepressant administration increases neurotrophin levels in the central and peripheral nervous system, leading to an increase of neuronal sprouting, reestablishment of neural networks and neurotransmitter levels. Injured peripheral nerves regenerate at very slow rates. However, the recovery of the hypogastric nerve in rodents after injury is significantly improved with neurotrophin administration. Accordingly, our goal was to determine whether treatment with the antidepressant fluoxetine affects catecholamine levels and neuronal function, after surgical denervation of the rat vas deferens. Noradrenaline levels in the denervated vas deferens were higher in fluoxetine-treated animals than in the vehicle-treated group, as measured by high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine/chronic administration/effects; Denervation; Antidepressants/long term use/experimental study; Adrenergic neurotransmission; Noradrenaline/neuronal release.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400757