Consulta: subjectFacets:"Depolimerização"
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Data/hora: 02/06/2024 09:45:21

Mild-acid hydrolysis of a native polysulfated fraction from Acanthophora muscoides generates sulfated oligosaccharides displaying in vitro thrombin generation inhibition
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
Acanthophora muscoides (Rhodophyta) has three polysulfated fractions    (Am-1, Am-2 and Am-3). Am-2 displayed anti-inflammation and serpin-independent anticoagulation; however, no effect of oligomers on thrombin-generation (TG) assay has been demonstrated. This study employed mild-acid hydrolysis to obtain low-molecular-size derivatives from Am-2 and compared in vitro inhibitory effects between intact Am-2 and its hydrolysates on a TG assay. The enzymatic extract was fractionated by DEAE-cellulose that revealed Am-2 eluted with 0.75-M NaCl containing sulfate (23%), hexoses (51%) and devoid of proteins, and indicating, by one-dimension nuclear magnetic resonance, structure of galactan similar to that of extract. The depolymerization with HCl (0.02 or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências Biológicas e Ambientais Rhodophyceae; Poli-aniônicos; Depolimerização; Trombina. Química de macromoléculas Rhodophyceae; Polyanionics; Depolymerization; Thrombin.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28257