Consulta: subjectFacets:"Dermatan sulfate"
Registros recuperados: 6
Data/hora: 07/06/2024 03:52:56

Structure and anticoagulant properties of sulfated glycosaminoglycans from primitive Chordates
Provedor de dados: 42
Autores: PAVÃO,MAURO S. G.
Dermatan sulfates and heparin, similar to the mammalian glycosaminoglycans, but with differences in the degree and position of sulfation were previously isolated from the body of the ascidian Styela plicata and Ascidia nigra. These differences produce profound effects on their anticoagulant properties. S. plicata dermatan sulfate composed by 2-O-sulfatedalpha-L-iduronic acid and 4-O-sulfated N-acetyl-beta-D-galactosamine residues is a potent anticoagulant due to a high heparin cofactor II activity. Surprisingly, it has a lower potency to prevent thrombus formation on an experimental model and a lower bleeding effect in rats than the mammalian dermatan sulfate. In contrast, A. nigra dermatan sulfate, also enriched in 2-O-sulfated alpha-L-iduronic acid, but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascidian; Heparin; Dermatan sulfate; Heparin cofactor II; Anticoagulant; Antithrombotic.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100007

The specificity of interactions between proteins and sulfated polysaccharides
Provedor de dados: 42
Autores: Mulloy,Barbara.
Sulfated polysaccharides are capable of binding with proteins at several levels of specificity. As highly acidic macromolecules, they can bind non-specifically to any basic patch on a protein surface at low ionic strength, and such interactions are not likely to be physiologically significant. On the other hand, several systems have been identified in which very specific substructures of sulfated polysaccharides confer high affinity for particular proteins; the best-known example of this is the pentasaccharide in heparin with high affinity for antithrombin, but other examples may be taken from the study of marine invertebrates: the importance of the fine structure of dermatan sulfate (DS) to its interaction with heparin cofactor II (HCII), and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sulfated polysaccharides; Fucans; Dermatan sulfate; Heparan sulfate; Heparin; Protein structure; Growth factors; Development.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400007

Galactosaminoglycans from normal myometrium and leiomyoma
Provedor de dados: 56
Autores: Berto,A.G.A.; Oba,S.M.; Michelacci,Y.M.; Sampaio,L.O.
In many tumors, the amount of chondroitin sulfate in the extracellular matrix has been shown to be elevated when compared to the corresponding normal tissue. Nevertheless, the degree of chondroitin sulfate increase varies widely. In order to investigate a possible correlation between the amount of chondroitin sulfate and tumor size, several individual specimens of human leiomyoma, a benign uterine tumor, were analyzed. The glycosaminoglycans from eight tumors were extracted and compared with those from the respective adjacent normal myometrium. The main glycosaminoglycan found in normal myometrium was dermatan sulfate, with small amounts of chondroitin sulfate and heparan sulfate. In leiomyoma, both dermatan sulfate and chondroitin sulfate were detected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Galactosaminoglycan; Glycosaminoglycan; Chondroitin sulfate; Dermatan sulfate; Leiomyoma; Myometrium.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500011

Dermatan sulfate reduces monocyte chemoattractant protein 1 and TGF-β production, as well as macrophage recruitment and myofibroblast accumulation in mice with unilateral ureteral obstruction
Provedor de dados: 56
Autores: Belmiro,C.L.R.; Gonçalves,R.G.; Kozlowski,E.O.; Werneck,A.F.; Takyia,C.M.; Leite-Jr.,M.; Pavão,M.S.G.
Selectins play an essential role in most inflammatory reactions, mediating the initial leukocyte-rolling event on activated endothelium. Heparin and dermatan sulfate (DS) bind and block P- and L-selectin function in vitro. Recently, we reported that subcutaneous administration of DS inhibits colon inflammation in rats by reducing macrophage and T-cell recruitment and macrophage activation. In the present study, we examined the effect of porcine intestinal mucosa DS on renal inflammation and fibrosis in mice after unilateral ureteral obstruction (UUO). Twenty-four adult male Swiss mice weighing 20-25 g were divided into 4 groups: group C (N = 6) was not subjected to any surgical manipulation; group SH (N = 6) was subjected to surgical manipulation but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans; Dermatan sulfate; Kidney; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700003

Collagens and proteoglycans of the corneal extracellular matrix
Provedor de dados: 56
Autores: Michelacci,Y.M.
The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea; Proteoglycan; Glycosaminoglycan; Collagen; Dermatan sulfate; Keratan sulfate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800009

Preparation and purification of Flavobacterium heparinum chondroitinases AC and B by hydrophobic interaction chromatography
Provedor de dados: 56
Autores: Aguiar,J.A.K.; Michelacci,Y.M.
Flavobacterium heparinum is a soil bacterium that produces several mucopolysaccharidases such as heparinase, heparitinases I and II, and chondroitinases AC, B, C and ABC. The purpose of the present study was to optimize the preparation of F. heparinum chondroitinases, which are very useful tools for the identification and structural characterization of chondroitin and dermatan sulfates. We observed that during the routine procedure for cell disruption (ultrasound, 100 kHz, 5 min) some of the chondroitinase B activity was lost. Using milder conditions (2 min), most of the chondroitinase B and AC protein was solubilized and the enzyme activities were preserved. Tryptic soy broth without glucose was the best culture medium both for bacterial growth and enzyme...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondroitinases; Mucopolysaccharidases; Dermatan sulfate; Chondroitin sulfate.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500007