Consulta: subjectFacets:"Diarrhea"
Registros recuperados: 62
Data/hora: 06/06/2024 03:32:23

Diarréia epidêmica dos suínos (PED).
Provedor de dados: 14
Autores: ZANELLA, J. R. C.; MORES, N.
bitstream/item/141042/1/final8035.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Doença animal; Diarréia; Suíno; Animal diseases; Diarrhea; Swine.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1040628

Listeria monocytogenes in HIV-infected patients in a hospital of Nova Iguaçu, Rio de Janeiro, Brazil
Provedor de dados: 80
Autores: Norberg,A. N.; Maure,E. A. P.; Svaiter,N.; Gonçalves,A. S.; Sanches,F. G.
A survey was carried out in a hospital of Nova Iguaçu, Rio de Janeiro, Brazil, in the period from July 1999 to March 2002, to determine the infection rate of Listeriamonocytogenes in HIV+ patients with diarrhea symptoms; 134 samples were processed by microbiological methods. The results demonstrated 12.68% of positive samples. However, no statistical differences were observed for age or sex in the studied group, suggesting that this microorganism should be regarded in the differential diagnosis of infectious processes in HIV+ patients in the area.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeriosis; HIV; Diarrhea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400016

Cryptosporidium SP in HIV-infected individuals attending a Brazilian University Hospital
Provedor de dados: 80
Autores: Langoni-Júnior,G.; Souza,L. R.
The aim of the current work was to evaluate the occurrence of Cryptosporidium sp in AIDS patients in a region of São Paulo State, Brazil. Patients were divided into groups according to CD4+ T lymphocyte count and use of potent antiretroviral treatment. Two hundred and ten fecal samples from 105 patients were fixed in 10% formalin and subjected to centrifuge formol-ether sedimentation. Slides were stained with auramine and confirmed by modified Ziehl-Neelsen. Cryptosporidiosis occurrence was 10.5% with no relationship among gender, age or the presence of diarrhea. The number of oocysts in all samples was small, independent of CD4+ T lymphocyte count, HIV plasma viral load, and presence of diarrhea. These results may be due to the reduced prevalence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Viral load; HIV; HAART; Cryptosporidium; CD4-positive T-lymphocytes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400005

Rotavirus gastroenteritis in a children's hospital specialized in craniofacial malformations
Provedor de dados: 80
Autores: Vieira,NA; Pereira,PCM; Carvalho,LR.
This study aimed to verify the relationship between acute diarrhea provoked by rotavirus and different indicators of craniofacial malformations. In the Hospital for Rehabilitation of Craniofacial Anomalies, 8,724 children with cleft lip and cleft palate were divided into the following groups: acute diarrhea and infection due to rotavirus (C1, n = 62), acute diarrhea (C2, n = 153) and without acute diarrhea (C3, n = 8,509). In C1, 29.03% of the cases consisted of hospital infections associated with the hospitalization period while 38.71% of the patients were aged less than six months. The percentage of children not having breastfed was significantly higher in acute diarrhea groups. Additionally, there was a seasonal prevalence of rotavirus infection between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Gastroenteritis; Diarrhea; Child.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200015

Phytogenic feed additives in piglets challenged with Salmonella Typhimurium
Provedor de dados: 96
Autores: Bruno,Daniel Gonçalves; Martins,Simone Maria Massami Kitamura; Parazzi,Larissa José; Afonso,Esther Ramalho; Del Santo,Tácia Antunes; Teixeira,Sergio de Mello Novita; Moreno,Andrea Micke; Moretti,Aníbal de Sant'Anna.
The effects of phytogenic feed additives on piglet performance and fecal score (FDD), as well as on lipid oxidation of pork meat were evaluated. One hundred and twenty crossbred weaned piglets were randomly assigned to six treatments according to a 2 × 3 factorial design with five replicates per treatment. Factors were: challenge with Salmonella Typhimurium at 35 days of age or no challenge, and three different additives (control (CTR), basal diet; phytogenic feed additives (PHY), basal diet plus 2000 ppm of phytogenic feed additives - Rosmarinus officinalis, Mentha piperita, Lippia sidoides and Porophyllum ruderale; and antimicrobial agent (ATB), basal diet plus 100 ppm of tylosin, 2000 ppm of zinc and colistin sulfate, 30 ppm in the pre-starter basal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Growth promoter; Lipid oxidation; Performance; Phytogenic compounds; Swine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000200009

Sources of organic trace minerals in diets for weaned piglets
Provedor de dados: 96
Autores: Mello,Gabriela de; Berto,Dirlei Antonio; Lo Tierzo,Vivian; Augusto,Regina Maria Nascimento; Silva,Anália Maria Ribeiro da; Trindade Neto,Messias Alves da; Villela,Cássio Cordeiro Ensá Junqueira; Girão,Lúcio Vilela Carneiro.
One hundred and twenty six piglets from a commercial genetic strain weaned at 21 days of age (6.11±0.42 kg) were used to evaluate the effects of supplementation levels of organic sources of trace minerals in the diets of weaned piglets on performance, occurrence of diarrhea, excretion of copper and zinc in the feces, and hematological parameters. A completely randomized block design was adopted, composed of six treatments (diets containing 100% of inorganic trace minerals premix at 3.00 kg/T; diets containing 0, 25, 50, 75 or 100% of organic trace minerals premix, equivalent to 0; 0.75; 1.50; 2.25 or 3.00 kg/T, respectively), seven replicates and three animals per plot. During the experimental period (from 21 to 63 days of age), the increasing levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Hematological parameters; Pigs; Performance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000800010

Probiotics and antibiotics as additives for sows and piglets during nursery phase
Provedor de dados: 96
Autores: Silva,Marcus Leonardo Figueiredo; Lima,José Augusto de Freitas; Cantarelli,Vinícius de Souza; Amaral,Níkolas de Oliveira; Zangerônimo,Márcio Gilberto; Fialho,Elias Tadeu.
The purpose of this study was to evaluate the effects of the use of probiotics in diets for sows and piglets on performance and morphophysiologic parameters and the incidence of diarrhea in piglets during 28 days after weaning. A total of 120 pigs weaned at 21 days of age from 24 litters whose sows (primiparous) received probiotics or antibiotics from the 94th day of gestation until weaning. It was used a complete randomized design in a 2 × 3 factorial (probiotics or antibiotics for sows and three diets for piglets - antibiotics, probiotics or probiotic-antibiotic combination) with four replicates of five animals to evaluate the performance and incidence of diarrhea. At 28 days of experiment, two animals from each plot were sacrificed for histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Early stage; Nutrition; Performance; Weaning.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001100019

Incidence of Campylobacter in pigs with and without diarrhea
Provedor de dados: 59
Autores: Modolo,José Rafael; Margato,Luiz Florêncio Fernandes; Gottschalk,Arnold Frederico; Lopes,Carlos Alberto de Magalhães.
Two hundred pigs (1- 21 weeks old), from five piggeries in São Paulo State, Brazil, were divided in two groups of 100 animals each, G1 with diarrhea and G2 without diarrhea. Campylobacter was recovered from 43% of G1 and 34% of G2 specimens, and was more frequently recovered from 0-4 week old piglets. C. coli was the most common species (44.2% in G1 and 32.4% in G2), followed by C. jejuni/coli (16.3% in G1 and 23.5% in G2). Campylobacter counts were significantly higher in G1 (&lt;FONT FACE="Symbol"&gt;£&lt;/FONT&gt; 108 UFC/g) than in G2 (&lt;FONT FACE="Symbol"&gt;£&lt;/FONT&gt; 104 UFC/g) (p &amp;lt; 0.01), which suggests that the bacterium may play a role at least in the aggravation of the diarrheic process.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Campylobacter; Diarrhea; Pigs.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100004

Detection of viral particles in feces of young dogs and their relationship with clinical signs
Provedor de dados: 59
Autores: Amo,Alicia N. del; Aprea,Adriana N.; Petruccelli,Miguel A.
Gross and light microscopic studies of 100 stool specimens of young dogs were carried out. Viral particles were detected in 31% of the analized samples using negative contrast electron microscopic diagnostic technique. Parvo-like virus, corona-like virus and other non-identified particles were observed in 17%, 7% and 2% of the samples, respectively. Parvo-like and corona-like viruses were found together in 5% of the samples. More than half (58.82%) of the positive parvo-like virus specimens were from dogs aged between 6 weeks and 6 months. 42.85% of the corona-like virus positive samples were detected in dogs between 6 weeks and 6 months and a similar percentage was found in dogs older than six months of age. Dual infections with parvo-like and corona-like...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parvo-like virus; Corona-like virus; Diarrhea; Dogs; Electron microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300009

Evaluation of growth performance and gastro-intestinal parameters on the response of weaned piglets to dietary organic acids
Provedor de dados: 42
Autores: GRECCO,HENRIQUE A.T.; AMORIM,ALESSANDRO B.; SALEH,MAYRA A.D.; TSE,MARCOS L.P.; TELLES,FILIPE G.; MIASSI,GABRIELA M.; PIMENTA,GUILHERME M.; BERTO,DIRLEI A.
ABSTRACT Two experiments (E) were carried out to evaluate the effects of fumaric acid and an acidifier blend [composed by calcium formate, calcium lactate and medium-chain fatty acids (capric and caprylic)] in piglet diets containing colistin (40 ppm) or halquinol (120 ppm) on performance, diarrhea incidence (E1), organs relative weight, pH values, intestinal morphometry and microbiota (E2). In E1, 192 and E2, 24 piglets weaned at 21-day-old were randomly assigned to blocks with 2x2 factorial arrangement of treatments [absence or presence of fumaric acid x absence or presence of acidifier blend], six replicates of eight (E1) and one piglet per pen (E2). For E1, the treatments were control (CD): no acidifier product + 40 ppm of colistin, FA: fumaric acid in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acidifiers; Diarrhea; Nutrition; Pig; Weaning.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100401

Maintenance enteral electrolyte solutions for neonatal calves: sodium acetate and osmolarity effects
Provedor de dados: 45
Autores: Lima,A.P.; Ribeiro Filho,J.D.; Ermita,P.A.N.; Baptista Filho,L.C.F.; Avanza,M.F.B.; Viana,R.B.; Santos,P.V.M.; Silva,M.O.; Monteiro,L.C.; Dantas,W.M.F.
ABSTRACT The use of hypotonic electrolytic solutions in enteral fluid therapy is still understudied in calves. The objective of the present study was to evaluate the effects of maintenance enteral electrolytic solutions with different concentrations of sodium acetate and different osmolarities in calves. For this, 18 Holstein calves, six male and 12 female, 20 days old and weighing around 52kg, were used. The animals were randomly divided into three groups and each group received one of the treatments. The three electrolytic solutions contained the same components in different concentrations, resulting in a hyposmotic, an isosmotic and a hyperosmotic solution. Each animal was maintained in enteral fluid therapy for 12 hours with infusion rate of 15mL kg-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Fluid therapy; Hypotonic solution; Volemia.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100018

Porcine circovirus-2 load in the gastrointestinal tract, mesenteric lymph node, tonsil, and feces of wasted pigs with and without diarrhea
Provedor de dados: 45
Autores: Zlotowski,P.; Corrêa,A.M.R; Castro,L.A; Koerich,P.K.V; Barcellos,D.E.S.N; Driemeier,D.
Relata-se a quantificação de PCV-2, por meio de PCR em tempo real, no trato gastrintestinal, linfonodo mesentérico, tonsilas e fezes de nove suínos refugo da fase de crescimento, provenientes de rebanhos com histórico de diarreia não responsiva ao tratamento com antibioticoterapia. Com base nos resultados histopatológicos e imunoistoquímicos, os animais foram classificados como afetados por PCV-2 (n=5) e não afetados (n=4). Foi observada diarreia em todos os animais do grupo afetado por PCV-2. Em todas as amostras testadas foi detectado PCV-2, no entanto, a carga viral observada nos tecidos, bem como a excreção nas fezes foi significantemente maior (P&lt;0.01) nos animais com diarreia. A maior concentração de PCV-2 foi observada nos linfonodos...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: PCV-2; Viral load; Diarrhea.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600036

Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum
Provedor de dados: 45
Autores: Figueiredo,H.C.P.; Lage,A.P.; Pereira Júnior,F.N.; Leite,R.C.
A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the blocks, each block consisting of two pregnant females (a vaccinated one and a control one) and their respective calves. In experiment A (farm 1), comprised of 18 blocks, the animals received a vaccine dose 30 days before delivery. In experiment B (farm 1), consisted of 26 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In experiment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Enterotoxigenic Escherichia coli; Vaccine; Diarrhea; K99; F41.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400001

Virulence factors of Escherichia coli isolated from diarrheic calves
Provedor de dados: 45
Autores: Rigobelo,E.C.; Gamez,H.J.; Marin,J.M.; Macedo,C.; Ambrosin,J.A.; Ávila,F.A.
One hundred seventy-three Escherichia coli strains isolated from calves from northwestern São Paulo State, having diarrhea were examined for the production of thermolabile (LT) and thermostable (ST) enterotoxins and for the presence of virulence factors associated with bovine colibacillosis. Eighty-five (49.1%) of the E.coli strains produced toxins; 53 isolates were detected as producing STa toxin, and 9 also produced LT toxin. By PCR, 23 isolates were shown to harbor only the LT-II gene. Nine (5.2%) isolates harbored Shiga toxin genes: four carried the stx2 gene, four the stx1 gene and one carried both. Three of the isolates showing stx1 also carried the eae gene. Among the E. coli isolates examined for susceptibility to 10 antimicrobial agents,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Diarrhea; Escherichia coli; Virulence factor; Antimicrobial agent.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300003

Avaliação clínica da infecção experimental de bezerros com Salmonella Typhimurium
Provedor de dados: 45
Autores: Ávila,L.G.; Silva,D.G.; Sato,R.A.; Fagliari,J.J.
The clinical conditions of healthy calves infected with 10(9) CFU of Salmonella Typhimurium were evaluated and the viability of the experimental model in disease induction was verified. Twelve male Holstein calves, 10 to 15-days old, were examined. They were allocated into two groups, control and experimentally infected. Animals were submitted to clinical examination after inoculation and at every 24 hours, during six days after infection. Samples of rectal swabs were collected for Salmonella Typhimurium isolation. Results showed that all animals had different degrees of diarrhea, with mucus and bleeding, 24 to 72 hours after experimental infection with Salmonella Typhimurium, accompanied by fever, but no signs of sepsis. The isolation of Salmonella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Salmonellosis; Diarrhea.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600043

Isolation of Clostridium perfringens and C. difficile in crab-eating fox ( Cerdocyon thous - Linnaeus 1776) from Northeastern Brazil
Provedor de dados: 45
Autores: Almeida,J.C.; Silva,R.O.S.; Lobato,F.C.F.; Mota,R.A.
ABSTRACT The aim of the present study was to isolate Clostridium perfringens and C. difficile in crab-eating fox (Cerdocyon thous) from Northeastern Brazil. Stool samples of 18 captive crab-eating foxes from four states of Northeastern Brazil (Alagoas, Bahia, Paraíba e Pernambuco) were collected and subjected to C. perfringens and C. difficile isolation. Suggestive colonies of C. perfringens were then analyzed for genes encoding the major C. perfringens toxins (alpha, beta, epsilon and iota), beta-2 toxin (cpb2), enterotoxin (cpe), and NetB- (netB) and NetF- (netF) encoding genes. C. difficile strains were analyzed by multiplex-PCR for a housekeeping gene (tpi), toxins A (tcdA) and B (tcdB) and a binary toxin gene (cdtB). Unthawed aliquots of stool samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Carnivores; Wild canids.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601709

Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli
Provedor de dados: 47
Autores: Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M.
Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction).
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006

Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves
Provedor de dados: 52
Autores: Stipp,Danilo Tancler; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Bodnar,Lívia; Alfieri,Amauri Alcindo.
Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Diarrhea; Bovine coronavirus; Cell culture; Molecular analyses.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700007

Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus
Provedor de dados: 52
Autores: Barry,Aline Fernandes; Alfieri,Alice Fernandes; Stipp,Danilo Tancler; Alfieri,Amauri Alcindo.
Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Diarrhea; BCoV; Rotavirus; SN-PCR; Co-infection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006

An outbreak of winter dysentery caused by bovine coronavirus in a high-production dairy cattle herd from a tropical country
Provedor de dados: 52
Autores: Takiuchi,Elisabete; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Filippsen,Patrícia; Alfieri,Amauri Alcindo.
Bovine coronavirus (BCoV) is a known cause of winter dysentery (WD) in adult cattle. The morbidity of the disease is high, that results in a significant decrease in milk production and consequently, economic losses. In the present study, we report on a classical outbreak of WD that affected a high-production Holstein dairy herd raised in a tropical country. The lactating batch included 154 cows, and 138 (90%) presented diarrhea in a short (nine days) period of time. Three (2%) cows died. The other batches of animals did not become ill. The evolution of the disease in the herd, including the clinical signs and epidemiological features, strongly suggested a WD case. Semi-nested PCR and RFLP confirmed that BCoV was the cause of the infection. Samples tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Dairy herd; Winter dysentery; Diarrhea; Bovine coronavirus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700008

Incidence of group A rotavirus in urban and rural areas of the city of Londrina-Brazil, from 1995 to 1997
Provedor de dados: 52
Autores: Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko.
Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006

Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil
Provedor de dados: 52
Autores: Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.
From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008

The infectivity of pig rotavirus in stools
Provedor de dados: 62
Autores: RAMOS,Ana Paula Dores; STEFANELLI,Carla Cristina; LINHARES,Rosa Elisa Carvalho; BRITO,Benito Guimarães de; NOZAWA,Carlos Mitihiko.
Rotaviruses are the major pathogen for both human and animal. They affect young animals in intensive rearing and cause great economic losses. This study intended to evaluate the infectivity of porcine rotavirus maintained for 32 months at approximately 10ºC. in the original stools specimens. Thirty stools specimens of 1-4-week-old piglets originating from breeding farms located in the southwest region of the Paraná State were selected for this study. They were randomly chosen from stools samples positive for rotavirus by polyacrylamide gel electrophoresis (PAGE) at the time of collection. The thirty stools samples maintained for 32 months at approximately 10ºC were re-tested by PAGE and 11 out of 30 were still positive showing the integrity of the eleven...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Rotavirus Infection; Diarrhea; Swine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000200007

Contribution to the study of diarrhea etiology in neonate dairy calves in São Paulo state, Brazil
Provedor de dados: 62
Autores: Langoni,Helio; Linhares,Antonio Carlos; Avila,Fernando Antonio de; Da Silva,Aristeu Vieira; Elias,Acacia Orieth.
Two hundred and three samples of feces of calves aged less than 30 days old from both sexes and from different properties and regions of the state of São Paulo were examined in the period of two years. Bacterial cultures were carried out in bovine 10% bloodagar and Levine mediums, incubated for up to 96 hours in aerobic conditions at 37°C, with the observation of colonies and morphological and biochemical study to characterize isolated microorganisms, or other tests, when pertinent. ELISA was performed for the Rotavirus research. The Cryptosporydium spp was surveyed and its parasitological examination was made. Results revealed the involvement of several enteropathogens alone and associated. Rotavirus was found in 51 (25.1%) samples, being 58.8% alone and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Calves; Etiology; Rotavirus; Cryptospodium.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000500004

Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC)
Provedor de dados: 55
Autores: Bouzari,Saeid; Aslani,Mohammad M; Oloomi,Mana; Jafari,Anis; Dashti,Amir.
Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2%)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Enteropathogenic Escherichia coli; Polymerase chain reaction.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400011

Antibiotics for the empirical treatment of acute infectious diarrhea in children
Provedor de dados: 55
Autores: Diniz-Santos,Daniel R.; Silva,Luciana R.; Silva,Nanci.
While the routine use of antibiotics for infectious diarrhea in children must be avoided, because it brings little benefit in most cases and is associated with the risk of increasing antimicrobial resistance, selected cases may require antimicrobial therapy, and the choice of the antimicrobial agent often has to be made empirically. Physicians prescribing antimicrobials in such a setting have not only to be aware of the most likely pathogens, but also of their characteristic antimicrobial susceptibility pattern and the safety profile of the various drugs. We reviewed the literature on the use of ampicillin, beta-lactamase inhibitors, trimethoprim-sulfamethoxazole, chloramphenicol, tetracyclines, nalidixic acid, fluoroquinolones, third-generation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Antibiotics; Children; Treatment.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300011

Epidemiological and microbiological aspects of acute bacterial diarrhea in children from Salvador, Bahia, Brazil
Provedor de dados: 55
Autores: Diniz-Santos,Daniel R.; Santana,José S.; Barretto,Junaura R.; Andrade,Maria Goreth M.; Silva,Luciana R.
In the few cases of acute childhood diarrhea that require antimicrobial therapy, the correct choice of the drug depends on detailed previous knowledge of local strains. In order to establish such parameters in our city, we reviewed the results of all 260 positive stool cultures of children between 0 and 15 years of age during two years at a pediatric tertiary care facility in Salvador, Brazil. Bacterial strains had been presumptively identified by culturing in selective media and by biochemical testing, and their antimicrobial susceptibility patterns were automatically detected by the MicroScan Walkaway System. Data about patients' sex and age, monthly distribution of the cases, pathogens isolated and their antimicrobial resistance patterns were recorded....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Antimicrobial; Resistance; Children; Epidemiology.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100013

Nosocomial diarrhea in the intensive care unit
Provedor de dados: 55
Autores: Marcon,Ana Paula; Gamba,Mônica Antar; Vianna,Lucila Amaral Carneiro.
We made an epidemiological case-control study to examine risk factors for the development of diarrhea in the intensive care unit (ICU) of a public hospital in Santo André, SP, from January to October 2002. Forty-nine patients with diarrhea (cases) and 49 patients without diarrhea (controls), matched for age and gender, were included in the study. A stool culture and enzyme immunoassays for Clostridium difficile toxins A and B were performed on fecal specimens from diarrhea patients. Fourteen of them presented positive cultures for Pseudomonas aeruginosa and 22 patients presented positive ELISA for Clostridium diffícile. Nosocomial diarrhea was associated with several factors, including use of antibiotics (P=0.001), use of ceftriaxone (P=0.001), presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nosocomial infections; Diarrhea; Antibiotics; Intensive care; Clostridium difficile.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600005

Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization
Provedor de dados: 55
Autores: Pereira,Luciane Aparecida; Raboni,Sonia Mara; Nogueira,Meri B; Vidal,Luine R; Almeida,Sergio Monteiro de; Debur,Maria C; Cruz,Cristina.
BACKGROUND AND OBJECTIVES: Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea. METHODS: 293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE). RESULTS: Rotavirus was detected in 34.8% of samples by LA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; Immunoenzyme techniques; Latex fixation tests; Electrophoresis; Polyacrylamide gel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300006

Common infectious diseases and skin test anergy in children from an urban slum in Northeast Brazil
Provedor de dados: 55
Autores: Castro,Melânia X.; Soares,Alberto M.; Fonsêca,Walter; Rey,Luís C.; Guerrant,Richard L.; Lima,Aldo A. M.
BACKGROUND: Acute respiratory infection (ARI), diarrheal disease (DD) and infective dermatitis (ID) are important causes of morbidity in children under five, in Northeast Brazil. Objectives: (a) to evaluate the morbidity of ARI, DD and ID; and (b) to determine their association with cellular immunity in poor urban children from Fortaleza, Brazil. MATERIALS AND METHODS: A prospective cohort study. At enrollment, multipuncture skin-tests (Multitest CMI) were performed and interpreted according to standard procedures. Children were followed for infectious diseases by weekly home visits. RESULTS: Seventy-one children aged 6 to 21 months were recruited in an ongoing cohort of newborns. A mean of 39 (6 to 63) home visits per child were made, which detected 184.5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Acute respiratory infection; Cellular immunity; Skin test; Child.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000600006

Incidence of diarrhea in children living in urban slums in Salvador, Brazil
Provedor de dados: 55
Autores: Melo,Maria Clotildes N. de; Taddei,José A.A.C.; Diniz-Santos,Daniel R.; Vieira,Camilo; Carneiro,Nadya B.; Melo,Rita Franca; Silva,Luciana R.
Diarrhea remains a major health issue in developing countries, with high morbidity and mortality rates. Determining the incidence of acute diarrhea in children and its associated factors is crucial to the planning of preventive approaches. The objective of this study was to determine the incidence of diarrhea and to assess some relevant associated factors to it in children younger than 40 months living in two slums of Salvador, Brazil. This is the first prospective cohort, community-based study that was performed in two periurban slums of Salvador, Brazil. Eighty-four children younger than 40 months were randomly selected and visited every other day for one year. The chi-square test was used to evaluate the occurrence of diarrhea and its associated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Children; Sewerage.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100019

Genetic analysis of the porcine group B rotavirus NSP2 gene from wild-type Brazilian strains
Provedor de dados: 56
Autores: Médici,K.C.; Barry,A.F.; Alfieri,A.F.; Alfieri,A.A.
Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Piglets; Diarrhea; Group B rotavirus; NSP2 gene; Phylogenetic analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100003

Prevalence of diarrheogenic Escherichia coli and rotavirus among children from Botucatu, São Paulo State, Brazil
Provedor de dados: 56
Autores: Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.
In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008

The Shiga toxin 2 B subunit inhibits net fluid absorption in human colon and elicits fluid accumulation in rat colon loops
Provedor de dados: 56
Autores: Creydt,V. Pistone; Miyakawa,M. Fernandez; Martín,F.; Zotta,E.; Silberstein,C.; Ibarra,C.
Shiga toxin (Stx)-producing Escherichia coli (STEC) colonizes the large intestine causing a spectrum of disorders, including watery diarrhea, bloody diarrhea (hemorrhagic colitis), and hemolytic-uremic syndrome. It is estimated that hemolytic-uremic syndrome is the most common cause of acute renal failure in infants in Argentina. Stx is a multimeric toxin composed of one A subunit and five B subunits. In this study we demonstrate that the Stx2 B subunit inhibits the water absorption (Jw) across the human and rat colonic mucosa without altering the electrical parameters measured as transepithelial potential difference and short circuit current. The time-course Jw inhibition by 400 ng/ml purified Stx2 B subunit was similar to that obtained using 12 ng/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemolytic-uremic syndrome; Diarrhea; Shiga toxin 2; B subunit; Water transport.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600004

Human group C rotavirus in children with diarrhea in the Federal District, Brazil
Provedor de dados: 56
Autores: Teixeira,J.M.S.; Camara,G.N.N.L.; Pimentel,P.F.V.; Ferreira,M.N.R.; Ferreira,M.S.R.; Alfieri,A.A.; Gentsch,J.R.; Leite,J.P.G.
Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Group C rotavirus; Diarrhea; HIV; RT-PCR; Probe hybridization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005

ApoE polymorphisms and diarrheal outcomes in Brazilian shanty town children
Provedor de dados: 56
Autores: Oriá,R.B.; Patrick,P.D.; Oriá,M.O.B.; Lorntz,B.; Thompson,M.R.; Azevedo,O.G.R.; Lobo,R.N.B.; Pinkerton,R.F.; Guerrant,R.L.; Lima,A.A.M.
A series of studies have shown that the heavy burdens of diarrheal diseases in the first 2 formative years of life in children living in urban shanty towns have negative effects on physical and cognitive development lasting into later childhood. We have shown that APOE4 is relatively common in shanty town children living in Brazil (13.4%) and suggest that APOE4 has a protective role in cognitive development as well as weight-for-height in children with heavy burdens of diarrhea in early childhood (64/123; 52%), despite being a marker for cognitive decline with Alzheimer’s and cardiovascular diseases later in life. APOE2 frequency was higher among children with heaviest diarrhea burdens during the first 2 years of life, as detected by PCR using the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; Malnutrition; Diarrhea; Children.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300005

Pathogenesis of Salmonella-induced enteritis
Provedor de dados: 56
Autores: Santos,R.L.; Tsolis,R.M.; Bäumler,A.J.; Adams,L.G.
Infections with Salmonella serotypes are a major cause of food-borne diseases worldwide. Animal models other than the mouse have been employed for the study of nontyphoidal Salmonella infections because the murine model is not suitable for the study of Salmonella-induced diarrhea. The microbe has developed mechanisms to exploit the host cell machinery to its own purpose. Bacterial proteins delivered directly into the host cell cytosol cause cytoskeletal changes and interfere with host cell signaling pathways, which ultimately enhance disease manifestation. Recently, marked advances have been made in our understanding of the molecular interactions between Salmonella serotypes and their hosts. Here, we discuss the molecular basis of the pathogenesis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella typhimurium; Enteritis; Diarrhea; Salmonellosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100002

Use of nucleotides in weanling rats with diarrhea induced by a lactose overload: effect on the evolution of diarrhea and weight and on the histopathology of intestine, liver and spleen
Provedor de dados: 56
Autores: Norton,R.; Leite,J.; Vieira,E.; Bambirra,E.; Moura,C.; Penna,G.; Penna,F.
Until recently, dietary sources of nucleotides were thought not to be essential for good nutrition. Certain states with higher metabolic demands may require larger amounts that cannot be provided by endogenous production. The objective of the present study was to determine the action of nucleotides on the recovery from lactose-induced diarrhea in weaned rats. Thirty-six weanling Fisher rats were divided into two groups. Group 1 received a standard diet and group 2 received a diet containing lactose in place of starch. On the 10th day, six animals per group were sacrificed for histopathological evaluation. The remaining animals were divided into two other subgroups, each with 6 animals, receiving a control diet, a control diet with nucleotides (0.05%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleotides; Diarrhea; Weight; Weanling rats.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200006

Measurement of intestinal permeability using mannitol and lactulose in children with diarrheal diseases
Provedor de dados: 56
Autores: Barboza Jr.,M.S.; Silva,T.M.J.; Guerrant,R.L.; Lima,A.A.M.
The excretion ratio of lactulose/mannitol in urine has been used to assess the extension of malabsorption and impairment of intestinal permeability. The recovery of lactulose and mannitol in urine was employed to evaluate intestinal permeability in children with and without diarrhea. Lactulose and mannitol probes were measured using high-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD). Two groups of solutions containing 60 µM sugars were prepared. Group I consisted of glucosamine, mannitol, melibiose and lactulose, and group II of inositol, sorbitol, glucose and lactose. In the study of intra-experiment variation, a sample of 50 µl from each group was submitted to 4 successive determinations. The recovered amounts and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Permeability test; Mannitol; Lactulose; HPLC-PAD; Diarrhea.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200008

Antimicrobial susceptibility of Campylobacter sp strains isolated from calves with and without diarrhea in Minas Gerais state, Brazil
Provedor de dados: 58
Autores: Miranda,Karina Leite; Lage,Andrey Pereira.
The antimicrobial susceptibility of 25 Campylobacter sp strains isolated from calves with and without diarrhea - 7 C. coli, 16 C. fetus and 2 C. jejuni was studied by the disk diffusion method. Eleven antimicrobial agents were tested amikacin, ampicillin, kanamycin, chloramphenicol, erythromycin, gentamicin, neomycin, nitrofurantoin, penicillin G, tetracycline and sulfamethoxazole-trimethoprim. All Campylobacter sp strains were susceptible to amikacin, ampicillin, chloramphenicol, erythromycin, gentamicin, neomycin and nitrofurantoin. Three strains were moderately susceptible to kanamycin (2 C. coli and 1 C. fetus). All the strains were resistant to penicillin G. Two C. fetus strains were moderately susceptible to sulfamethoxazole-trimethoprim and 1 C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter sp; Calf; Diarrhea; Antimicrobial agents; Susceptibility.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200032

Campylobacter jejuni occurrence in chicken fecal samples from small properties in Pelotas, southern of Brazil
Provedor de dados: 58
Autores: Gomes,Fabiane R.; Curcio,Bruna R.; Ladeira,Sílvia R.L.; Fernández,Heriberto; Meireles,Mário Carlos Araújo.
The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni; Thermotolerant species; Diarrhea; Chicken.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300032

Identification of enteric viruses circulating in a dog population with low vaccine coverage
Provedor de dados: 58
Autores: Alves,Christian D.B.T.; Granados,Oscar F.O.; Budaszewski,Renata da F.; Streck,André F.; Weber,Matheus N.; Cibulski,Samuel P.; Pinto,Luciane D.; Ikuta,Nilo; Canal,Cláudio W.
ABSTRACT Although the use of vaccines has controlled enteric diseases in dogs in many developed countries, vaccine coverage is still under optimal situation in Brazil. There is a large population of nonimmunized dogs and few studies about the identification of the viruses associated with diarrhea. To address this situation, stool samples from 325 dogs were analyzed by polymerase chain reaction for the detection of common enteric viruses such as Canine adenovirus (CAdV), Canine coronavirus (CCoV), Canine distemper virus (CDV), Canine rotavirus (CRV) and Carnivorous protoparvovirus 1 (canine parvovirus 2; CPV-2). At least one of these species was detected in 56.6% (184/325) of the samples. The viruses detected most frequently in either diarrheic or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distemper; Parvovirus; Diarrhea; Dog; Co-infection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400790

Antimicrobial susceptibility of Clostridium perfringens isolated from piglets with or without diarrhea in Brazil
Provedor de dados: 58
Autores: Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Pires,Prhiscylla Sadanã; Cruz Júnior,Eduardo Coulaud da Costa; Albefaro,Isabella Silva; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria.
The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors related to the antimicrobial resistance of C. perfringens.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium perfringens; Piglet; Diarrhea; Antibiotic.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300027

Clinical importance and representation of toxigenic and non-toxigenic Clostridium difficile cultivated from stool samples of hospitalized patients
Provedor de dados: 58
Autores: Stojanovic,Predrag; Kocic,Branislava; Stojanovic,Miodrag; Miljkovic-Selimovic,Biljana; Tasic,Suzana; Miladinovic-Tasic,Natasa; Babic,Tatjana.
The aim of this study was to fortify the clinical importance and representation of toxigenic and non-toxigenic Clostridium difficile isolated from stool samples of hospitalized patients. This survey included 80 hospitalized patients with diarrhea and positive findings of Clostridium difficile in stool samples, and 100 hospitalized patients with formed stool as a control group. Bacteriological examination of a stool samples was conducted using standard microbiological methods. Stool sample were inoculated directly on nutrient media for bacterial cultivation (blood agar using 5% sheep blood, Endo agar, selective Salmonella Shigella agar, Selenite-F broth, CIN agar and Skirrow's medium), and to selective cycloserine-cefoxitin-fructose agar (CCFA) (Biomedics,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clostridium difficile; Diarrhea; Toxins.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100023

Relationship among Shigella spp. and enteroinvasive Escherichia coli (EIEC) and their differentiation
Provedor de dados: 58
Autores: Ud-Din,Abu; Wahid,Syeda.
Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similarity. Shigella spp. could be easily differentiated from E. coli, but difficulties observed for the EIEC-Shigella differentiation as both show similar biochemical traits and can cause dysentery using the same mode of invasion. Sequencing of multiple housekeeping genes indicates that Shigella has derived on several different occasions via acquisition of the transferable forms of ancestral virulence plasmids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; E. coli; Shigella; Real-time PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400002

Distribution, detection of enterotoxigenic strains and antimicrobial drug susceptibility patterns of Bacteroides fragilis group in diarrheic and non-diarrheic feces from Brazilian infants
Provedor de dados: 58
Autores: Ferreira,Débora Paula; Silva,Vânia Lúcia; Guimarães,Danielle Aparecida; Coelho,Cíntia Marques; Zauli,Danielle Alves Gomes; Farias,Luiz Macêdo; Carvalho,Maria Auxiliadora Roque; Diniz,Claudio Galuppo.
Despite the importance of gastrointestinal diseases and their global distribution, affecting millions of individuals around the world, the role and antimicrobial susceptibility patterns of anaerobic bacteria such as those in the Bacteroides fragilis group (BFG) are still unclear in young children. This study investigated the occurrence and distribution of species in the BFG and enterotoxigenic strains in the fecal microbiota of children and their antimicrobial susceptibility patterns. Diarrheic (n=110) and non-diarrheic (n=65) fecal samples from children aged 0-5 years old were evaluated. BFG strains were isolated and identified by conventional biochemical, physiological and molecular approaches. Alternatively, bacteria and enterotoxigenic strains were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteroides fragilis group; ETBF; Antimicrobial susceptibility; Diarrhea.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300010

Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil
Provedor de dados: 58
Autores: Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia.
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015

Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken
Provedor de dados: 58
Autores: Tamehiro,Claudia Yurika; Alfieri,Alice Fernandes; Médici,Cristina; Alfieri,Amauri Alcindo.
Segmented double-stranded RNA (dsRNA) viruses were identified by polyacrylamide gel electrophoresis (PAGE) technique in fecal samples from broiler chicken. A total of 378 fecal samples from 1-7 weeks old chickens were analyzed. dsRNA with migration profile characteristic of avian rotavirus (AvRV), reovirus (ARV) or picobirnavirus (PBV) was identified in 32 (8.5%), 7 (1.8%) and 13 (3.4%) samples, respectively. AvRV and ARV occurred more frequently in chickens up to 1 month old and were related with enteritis signs. Considering only fecal samples of chickens with diarrhea, the AvRV was detected in 37.8% (14/37) and the ARV in 13.5% (5/37) of analyzed samples. AvRV was identified in only 1.5% (4/274) and ARV was not detected in normal feces collected from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avian rotavirus; Avian reovirus; Avian picobirnavirus; Broiler chickens; Diarrhea; PAGE.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400013

Inhibition of enterotoxigenic Escherichia coli (ETEC) adhesion to Caco-2 cells by human milk and its immunoglobulin and non-immunoglobulin fractions
Provedor de dados: 58
Autores: Oliveira,Inaiara R. de; Bessler,Heidi C.; Bao,Sonia N.; Lima,Renato de L.; Giugliano,Loreny G.
Enterotoxigenic Escherichia coli (ETEC) is the most common cause of diarrhea in children in developing countries and among travelers to ETEC endemic areas. ETEC diarrhea is caused by colonization of the small intestine mediated by colonization factor (CF) antigens, and subsequent elaboration of enterotoxins. Breast feeding has been related to protection against enteric infections. The protective effect of human milk can be ascribed to its immunoglobulin content, specially secretory immunoglobulin A (sIgA), and to non-immunoglobulin components such as free oligosaccharides, glycoproteins and glycolipids. In this study we investigated the effect of whole human milk and its fractions immunoglobulin and non-immunoglobulin on the adherence of ETEC strains...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Adherence inhibition; Escherichia coli enterotoxigenic; Colonization factor antigen; Human milk.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100018

Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil
Provedor de dados: 58
Autores: Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa.
Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009

Use of single-enzyme amplified fragment length polymorphism for typing Clostridium perfringens isolated from diarrheic piglets
Provedor de dados: 58
Autores: Shinya,Luciane Tieko; Baccaro,Maria Regina; Moreno,Andrea Micke.
Clostridium perfringens is an important pathogen in human and veterinary medicine. In swine, the agent is responsible for necrotic enteritis and enterotoxemia characterized by diarrhea, weight loss, delayed development and, in some cases, death. In the present study amplified fragment length polymorphism analyses (AFLP) was used to characterize 54 C. perfringens strains isolated from swine presenting diarrhea. Analysis of the results showed 29 distinct profiles with discriminatory index equal to 0.97. Partial correlation between the origin of the isolates and groups was drawn, and correlation was possible in only 18.5% of the samples. Characterization of the strains in biotypes (A, B, C, D and E), production of beta-2 toxin and enterotoxin were performed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Diarrhea; Piglets; Clostridium perfringens.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300034

Diarrheagenic Escherichia coli
Provedor de dados: 58
Autores: Gomes,Tânia A.T.; Elias,Waldir P.; Scaletsky,Isabel C.A.; Guth,Beatriz E.C.; Rodrigues,Juliana F.; Piazza,Roxane M.F.; Ferreira,Luís C.S.; Martinez,Marina B.
ABSTRACT Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Diarrhea; Pathogenic mechanisms; Virulence factor; Epidemiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500003

Isolation of Shiga toxin-producing Escherichia coli (STEC) serotype 091:H21 from a child with diarrhea in Porto Alegre city, RS, Brazil
Provedor de dados: 58
Autores: Cantarelli,Vlademir; Nagayama,Kenichi; Takahashi,Akira; Honda,Takeshi; Cauduro,Paulo F.; Dias,Cícero A.G.; Mezzari,Adelina; Brodt,Teresa.
We describe the isolation of one strain of Shiga toxin-producing Escherichia coli O91:H21 from a child with diarrhea in the city of Porto Alegre, RS, Brazil. Considering the pathogenic potential of STEC, these organisms should be looked for more carefully among our population.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterohemorrhagic E. coli; STEC; Enteroaggregative E. coli; Enteropathogenic E. coli; Diarrhea.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400005

Distribution of non-LEE-encoded type 3 secretion system dependent effectors in enteropathogenic Escherichia coli
Provedor de dados: 58
Autores: Salvador,Fábia A.; Hernandes,Rodrigo T.; Vieira,Mônica A.M.; Rockstroh,Anna C.; Gomes,Tânia A.T.
Enteropathogenic Escherichia coli (EPEC) are important human gastroenteritis agents. The prevalence of six non-LEE genes encoding type 3 translocated effectors was investigated. The nleC, cif and nleB genes were more prevalent in typical than in atypical EPEC, although a higher diversity of genes combinations was observed in atypical EPEC.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli (EPEC); Type 3 secretion system; Pathogenicity; Diarrhea.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300014

Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Gabardo,Michelle de Paula; Oliveira,Juliana Saes Vilaça de; Lobato,Francisco Carlos Faria; Guedes,Roberto Maurício Carvalho.
Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lawsonia intracellularis; Canine; Proliferative enteropathy; Diarrhea.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901619

Virulence factors of  Escherichia coli in relation to the importance of vaccination in pigs
Provedor de dados: 65
Autores: Pereira,Daniele Araujo; Vidotto,Marilda Carlos; Nascimento,Karla Alvarenga; Santos,Anne Caroline Ramos dos; Mechler,Marina Lopes; Oliveira,Luís Guilherme de.
ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) is the major cause of diarrhea in newborn and weaned pigs. Bacteria adhesion to the host cell is considered a specific phenomenon among fimbrial and non-fimbrial adhesins with their respective receptors on enterocytes. Enteric disorders are related with the fimbriae F4 (K88), F5 (K99), F6 (987P), F41, and F18. In addition to ETEC, another category of  E. coli , porcine pathogenic  E. coli (PEPEC),can cause diarrhea in pigs; it produces the porcine attaching and effacing-associated (Paa) adhesin in, which is capable to cause a typical lesion known as an attaching and effacing (A/E) lesion. Immunization of sows with adhesin is important to stimulate the production of antibodies and their subsequent transfer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colibacillosis; Diarrhea; Vaccines; Swine; Colostrum.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801430

Virulence factors and phylotyping of Escherichia coli isolated from non-diarrheic and diarrheic water buffalo calves
Provedor de dados: 65
Autores: Coura,Fernanda Morcatti; Diniz,Soraia de Araújo; Silva,Marcos Xavier; Oliveira,Cairo Henrique Sousa de; Mussi,Jamili Maria Suhet; Oliveira,Camila Stefanie Fonseca de; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
ABSTRACT:  This study aimed to determine the virulence factors, phylogenetic groups, and the relationships between pathovars and phylogenetic groups of E. coli strains isolated from feces of buffalo calves. A total of 217 E. coli strains were obtained from feces after culture and were screened by PCR for detection of virulence factors EAST-1, enterohemolysin, Saa, CNF2, F41, F5, STa, intimin, Stx1 and Stx2. One hundred and thirty-four isolates were positive for one or more virulence factors: eighty-four from diarrheic animals, and fifty from non-diarrheic calves. The pathovars of E. coli identified in diarrheic feces were ETEC (F5+) (2/84), NTEC (16/84), STEC (20/84), EPEC (3/84), EHEC (3/84), and EAEC (EAST-1+) (33/84). Pathovars identified in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo calves; Diarrhea; Escherichia coli; Pathovars; Phylogenetic group.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500451

Enteral fluid therapy in neonatal calves and features of commercially available electrolyte solutions in Brazil
Provedor de dados: 65
Autores: Bregadioli,Gabriela de Castro; Pereira,Priscilla Fajardo Valente; Flaiban,Karina Keller Marques da Costa; Ribeiro Filho,José Dantas; Lisbôa,Júlio Augusto Naylor.
ABSTRACT: According to the World Health Organization, the development of orally replacement fluids and electrolytes was one of the most significant advances of the twentieth century, markedly reducing mortality from diarrheal children. In veterinary medicine, oral electrolyte solutions (OES) are routinely used to treat diarrheic neonatal calves in order to correct water, electrolyte and acid base imbalances. In North America, the use of OES is routine and there are at least 20 commercial alternatives. Use of OES has increased in recent years in Brazil, but just five commercial products are currently available. The OES should contain, in suitable concentrations, electrolytes, glucose and an alkalizing agent to ensure its therapeutic efficacy. This review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Electrolyte imbalance; Diarrhea; Oral electrolyte solution; Dehydration; Acid base imbalance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700603

Propolis extract in tshe diet of weaned piglets
Provedor de dados: 65
Autores: Gonçalves,Liliane Maria Piano; Kiefer,Charles; Silva,Camilla Mendonça; Leal,Cássia Rejane Brito; Alencar,Stephan Alexander da Silva; Carvalho,Kelly Cristina Nunes; Rufino,Luciana Moura.
ABSTRACT:  This study was conducted to assess the use of propolis ethanolic extract as an alternative to performance-enhancing antibiotics for piglets. Seventy piglets weaned at 21 days of age, with initial weights of 7.3±0.4kg and final weights of 24.9±1.2kg, were randomly assigned to five diets: positive control (diet with 200ppm antibiotic); negative control (diet without growth promoter); and diets with 100, 500, and 1,000ppm of brown propolis ethanolic extract, with seven replicates of two piglets each. The duration of the experimental period was 35 days. The diets did not influence (P&gt;0.05) daily feed intake, daily weight gain, final weight, and feed conversion of animals. The bacteriological profile of the nasal swab presented a predominance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobials; Diarrhea; Ethanolic extract; Vegetable extract.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000100702

Clostridium perfringens: a review of the disease in pigs, horses and broiler chickens
Provedor de dados: 65
Autores: Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria.
Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027

Salmonella Typhimurium - associated meningoencephalomyelitis in a foal
Provedor de dados: 65
Autores: Oliveira,Jéssica Guerra de; Ramos,Carolina Pantuzza; Rocha,Izabela de Assis; Marcelino,Sóstenes Apolo Correia; Pierezan,Felipe; Palhares,Maristela Silveira; Maranhão,Renata de Pino Albuquerque; Silva,Rodrigo Otávio Silveira; Teixeira,Raffaella Bertoni Cavalcanti.
ABSTRACT: A 10-day old foal presented with a history of acute recumbency and generalized weakness, that progressed to seizure episodes and death. Post mortem examination revealed necrotizing and purulent omphalophlebitis and fibrinopurulent meningoencephalomyelitis. Salmonella Typhimurium was isolated from the central nervous system and determined to be the cause of the meningoencephalomyelitis. Due to the lack of evidence of gastrointestinal disease, the umbilical cord was considered the most likely portal of entry of the bacteria. The isolated S. Typhimurium was resistant to ampicillin and cephalotin, and partially resistant to enrofloxacin. These drugs are commonly used in the treatment of salmonellosis. This is the first report of S. Typhimurium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Salmonellosis; Omphalophlebitis; Meningoencephalitis; Diarrhea.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800451

Prevention of  Clostridium difficile infection in hamsters using a non-toxigenic strain
Provedor de dados: 65
Autores: Oliveira Júnior,Carlos Augusto de; Silva,Rodrigo Otávio Silveira; Diniz,Amanda Nádia; Pires,Prhiscylla Sadanã; Lobato,Francisco Carlos Faria; Assis,Ronie Antunes de.
ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Clostridium difficile- associated diseases; Colitis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853