Consulta: subjectFacets:"Dietary selenium deficiency"
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Data/hora: 02/06/2024 11:58:00

Antioxidant dietary deficiency induces caspase activation in chick skeletal muscle cells
Provedor de dados: 56
Autores: Nunes,V.A.; Gozzo,A.J.; Juliano,M.A.; Cerqueira César,M.; Sampaio,M.U.; Sampaio,C.A.M.; Araújo,M.S.
Apoptosis and necrosis are two distinct forms of cell death that can occur in response to different agents and stress conditions. In order to verify if the oxidative stress induced by dietary selenium and vitamin E deficiencies can lead muscle cells to apoptosis, one-day-old chicks were reared using diets differing in their vitamin E (0 or 10 IU/kg) and selenium (0 or 0.15 ppm) supplementation. Chick skeletal muscle tissue was obtained from 28-day-old animals and used to verify apoptosis occurrence based on caspase activity detection and DNA fragmentation. Antioxidant deficiency significantly increased caspase-like activity assessed by the hydrolysis of fluorogenic peptide substrates (Abz-peptidyl-EDDnp) at lambdaexc = 320 nm and lambdaem = 420 nm....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Caspase activation; Apoptosis; Dietary selenium deficiency; Dietary vitamin E deficiency; Internucleosomal DNA fragmentation; Caspase substrate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800010