Consulta: subjectFacets:"Differentiation"
Registros recuperados: 47
Data/hora: 06/06/2024 00:44:41

Single embryoid body formation in a multi-well plate
Provedor de dados: 69
Autores: Ezekiel,Uthayashanker R; Muthuchamy,Mariappan; Ryerse,Jan S; Heuertz,Rita M.
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES...
Tipo: Journal article
Palavras-chave: Differentiation; EB; ES; Murine embryonic stem cell.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200015

Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
Provedor de dados: 74
Autores: Mittmann,Josane; Kerkis,Irina; Kawashima,Cinthia; Sukoyan,Marina; Santos,Enrico; Kerkis,Alexandre.
We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse ES-somatic cell hybrids; Karyotype; Pluriopotency; Differentiation; Embryoid bodies.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100019

The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
Provedor de dados: 74
Autores: Mansouri,Vahid; Salehi,Mohammad; Nourozian,Mohsen; Fadaei,Fatemeh; Farahani,Reza Mastery; Piryaei,Abbas; Delbari,Ali.
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differentiation; Germ cells; Nuclear transfer embryonic stem cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220

Особенности формирования хроматофорного комплекса камбалы калкан Psetta maxima var. maeotica в онтогенезе в зависимости от пищевой цепи.
Provedor de dados: 13
Autores: Ханайченко, А. Н.; Битюкова, Ю. Е.
Сравнение влияния пищевой цепи на развитие пигментации (хроматофорного комплекса) у камбалы калкан Psetta maxima var. maeotica проводили от начала экзогенного питания до начала метаморфоза (3 - 18 сут) и от начала до последней стадии метаморфоза (18 – 60 сут). Группы калкана (в двух повторностях, из однородных партий икры) содержали в разных пищевых цепях: либо в типично морской, состоящей из культивируемых каланоидных копепод, Acartia tonsa (группа MZ), либо в искусственной, состоящей из солоноватоводных коловраток Brachionus plicatilis и метанауплиев Artemia  franciscana (группа BZ). Для аппроксимации  жирнокислотного состава применяли единую схему насыщения, в результате которой у всех кормовых организмов были близкие значения содержания и соотношения...
Tipo: Journal Contribution
Palavras-chave: Калкан; Пищевая цепь; Пигментация; Меланофор; Липофор; Гуанофор; Дифференциация; Black Sea turbot; Food web; Pigmentation; Melanophore; Lipophore; Guanophore; Differentiation; Харчовий ланцюг; Пігментація; Меланофор; Ліпофор; Гуанофор; Діфференціація.
Ano: 2007
URL: http://repository.ibss.org.ua/dspace/handle/99011/849

Di and tripeptides from marine sources can target adipogenic process and contribute to decrease adipocyte number and functions
Provedor de dados: 5
Autores: Ben Henda, Yesmine; Laamari, Mariem; Lanneluc, Isabelle; Travers, Marie-agnes; Agogue, Helene; Arnaudin, Ingrid; Bridiau, Nicolas; Maugard, Thierry; Piot, Jean-marie; Sannier, Frederic; Bordenave-juchereau, Stephanie.
The effect of 11 marine-derived cryptides was investigated on proliferation, differentiation and maturation of human white pre-adipocytes (HWP). They were all formerly identified as potent Angiotensin-Converting-Enzyme inhibitors.Val-Trp (VW),Val-Tyr (VY), Lys-Tyr (KY), Lys-Trp (KW), Ile-Tyr (IY), Ala-Pro (AP),Val-Ile-Tyr (VIY), Leu-Lys-Pro (LKP), Gly-Pro-Leu (GPL), Ala-Lys-Lys (AKK) and Val-Ala-Pro (VAP) were previously found in fish products and coproducts as well as other marine resources like wakame. Treatment with AP, VAP and AKK greatly affected viability of HWP during the proliferation period while KW and VW treatment reduced the number of viable cells during the differentiation stage. A GPL and IY incubation during the differentiation stage allowed...
Tipo: Text
Palavras-chave: Bioactive peptides; Obesity; Human white pre-adipocytes'; Proliferation; Differentiation; Adipocyte differentiation markers.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/38014/36308.pdf

Tumorhead distribution to cytoplasmic membrane of neural plate cells is positively regulated by Xenopus p21-activated kinase 1 (X-PAK1)
Provedor de dados: 5
Autores: Wu, Chuan-fen; Delsert, Claude; Faure, Sandrine; Traverso, Edwin E.; Kloc, Malgorzata; Kuang, Jian; Etkin, Laurence D.; Morin, Nathalie.
Tumorhead (TH) regulates neural plate cell proliferation during Xenopus early development, and gain or loss of function prevents neural differentiation. TH shuttles between the nuclear and cytoplasmic/cortical cell compartments in embryonic cells. In this study, we show that subcellular distribution of TH is important for its functions. Targeting TH to the cell cortex/membrane potentiates a TH gain of function phenotype and results in neural plate expansion and inhibition of neuronal differentiation. We have found that TH subcellular localization is regulated, and that its shuttling between the nucleus and the cell cortex/cytoplasm is controlled by the catalytic activity of p21-activated kinase, X-PAK1. The phenotypes of embryos that lack, or have excess,...
Tipo: Text
Palavras-chave: Actin cytoskeleton; Differentiation; Proliferation; Neural plate; P21 activated kinase; Tumorhead.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2996.pdf

Olivines in main-group pallasites: magma-ocean cumulates or partial melting residues?
Provedor de dados: 5
Autores: Barrat, Jean-alix; Ferrière, L.
Main-group pallasites (MGPs) are meteorites mainly composed of Fe-Ni metal and olivines, the latter being considered as one of the largest sampling of extraterrestrial mantle material available for study on Earth. We analysed the rare earth element (REE) concentrations of olivines from six MGPs to understand better the processes of formation of their parent mantle. All the investigated samples display very low REE abundances, and enrichments in both light REEs and heavy REEs. We interpret the light REE enrichments as a fingerprint of terrestrial contamination. The least contaminated olivines have higher heavy REE enrichments than those inferred for olivines directly crystallised in a magma ocean. Such enrichments in heavy REEs are possible if the mantle of...
Tipo: Text
Palavras-chave: Pallasite; Early solar system; Trace elements; Rare earth elements; Differentiation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79770/82569.pdf

Olfactory Epithelium as an Infinitive Source of Neural Stem Cells for Derivation of Inner Ear Hair Cells
Provedor de dados: 77
Autores: Bahmani,Tahere; Gholami,Samira; Mostafaie,Ali; Karimi,Ebrahim; Hoseinkhani,Zohreh; Azad,Taha; Mahdizadeh,Hamid; Mansouri,Kamran.
Surgical techniques for treatment of sensory neural hearing loss (SNHL) have unpredictable outcomes and in recent years cell therapy investigated for treatment of SNHL. Olfactory epithelium proceed neurogenesis during life time and provide an easily accessible source of neural stem cells. So the aim of this study was isolating neural stem cells from olfactory epithelium of rat and differentiation of these cells into hair cells of inner ear in vitro. The epithelium tissue of olfactory mucosa of rats were removed and digested by collagenase H. The digested tissue was cultured in flasks in suspension forms to create spheres. Spheres were passaged and from passage 2 spheres selected for differentiation. At this stage cells of spheres isolated from each other...
Tipo: Journal article
Palavras-chave: Olfactory mucosa; Neural stem cells; Hair cells; Differentiation; Rat.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200004

Cytological Aspects of the Differentiation of Barb Cells During the Formation of the Ramus of Feathers
Provedor de dados: 77
Autores: Alibardi,Lorenzo.
The present ultrastructural study on developing and regenerating feathers of chick and zebrafinch describes the ultrastructural changes that occur during the differentiation of barb cells that leads to the formation of the ramus of barbs. Differently from barbule and barb cortical cells that accumulate feather keratin, barb medullary cells undergo to lipid degeneration. Eventually, lipids disappear and medullary cells become empty cavities in the central part of the ramus. In barb medullary cells feather keratin is accumulated in few peripheral bundles that merge with those of cortical cells to fom the wall of the ramus. The latter is joined with branching barbules. The process that controls the transition from keratin-synthesizing to lipid-producing barb...
Tipo: Journal article
Palavras-chave: Feathers; Barbs; Differentiation; Lipidization; Ultrastructure.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000100010

Morphometrics and cytogenetics of Gracilinanus Agilis and Cryptonanus spp. (Didelphimorphia: Didelphidae) from central and northeastern Brazil
Provedor de dados: 81
Autores: Garcia,João P.; Oliveira,João A.; Corrêa,Margaret M. O.; Pessôa,Leila M.
Species of the didelphid genera Gracilinanus and Cryptonanus are morphologically and cytogenetically very similar. Several qualitative characters, some of which exhibit intraspecific polymorphisms, have been used to distinguish these genera, but more data are needed to characterize them better. Samples of G. agilis and Cryptonanus spp. from nine localities in central and northeastern Brazil were analyzed. Multivariate analyses of craniodental measurements and descriptive statistics of external body measurements indicate that G. agilis is conspicuously larger than Cryptonanus spp., and that general size is the main factor distinguishing these forms. Size differences can be combined with qualitative characters making the differentiation between G. agilis and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differentiation; General size; Karyotypes; Mouse opossums.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832010000100006

Electrophoretic protein pattern and acid phosphatase activity in the midgut extracts of Apis mellifera L. (Hymenoptera: Apidae) during metamorphosis
Provedor de dados: 82
Autores: Cavalcante,Vagner M.; Cruz-Landim,Carminda da.
The electrophoretic pattern of proteins and the activity of acid phosphatase were described in the midgut extracts during the post-embryonic stages of Apis mellifera L. in order to stablish a correlation with the metamorphosis events. The results show the greatest variation in proteic electrophoretical bands during prepupal stage and the smallest variation during the brown eyed pupae stage. The acid phosphatase activity was higher during the larval last instar and lower in white eyed pupae. The greatest variety of bands during the prepupal stage coincides with the histolysis of the larval midgut epithelium and the lowest variability in the brown eyed pupa coincides with the end of the midgut differentiation. In another hand, the greatest phosphatase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differentiation; Enzymes; Histolysis; Larva; Prepupa; Post-embryonic development; Pupa.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200007

The Effects of Imbalanced Competition on Demonstration Strategies
Provedor de dados: 31
Autores: Heiman,   Amir; Ofir,   Chezy.
This paper analyzes the effect of competition on product demonstration decisions. Pre-purchase product demonstration enables marketers to differentiate products that are ex-post differentiated but are judged according to perceived fit, rather than actual fit, due to pre-purchase consumer uncertainty. Imbalanced competition accompanied by fit uncertainty motivates the follower to offer demonstrations to avoid a price war. This paper explores the conditions that lead the leader to retaliate. In addition to effects on quantity, competition may increase the quality of demonstrations offered by the leader. We analyze a business case, showing that competition may increase the demonstration intensity and that the leading manufacturer’s response to changes in...
Tipo: Working or Discussion Paper
Palavras-chave: Imbalanced competition; Product demonstration; Differentiation; Test-drive; Price war; Political Economy; Production Economics.
Ano: 2010
URL: http://purl.umn.edu/93131

Connecting Product Attributes with Emotional Benefits. Analysis of a Mediterranean product across consumer age segments
Provedor de dados: 31
Autores: Barrena Figueroa,   Ramo; Sanchez Garcia,   Mercedes.
Due to a high level of product substitution in the food market, it often proves difficult to strike a balance between supply and demand. This is especially true in sectors with a such high level of competition and product differentiation as the wine producing sector. Faced with the difficulty of differentiating the product in terms of its technical characteristics, quality and price, therefore, a useful alternative is to explore what consumers perceive to be its "emotional benefits", since these have been shown to have a decisive effect on consumer purchasing decisions. For marketing purposes, an understanding of how consumers' personality traits condition their choice of products can help manufacturers to improve their strategic positioning in the market....
Tipo: Conference Paper or Presentation
Palavras-chave: Wine; Consumer behaviour; Laddering; Emotions; Differentiation; Demand and Price Analysis.
Ano: 2007
URL: http://purl.umn.edu/9435

BUYER POWER THROUGH PRODUCER'S DIFFERENTIATION
Provedor de dados: 31
Autores: Chambolle,   Claire; Villas-Boas,   Sofia Berto.
This paper shows that retailers may choose to offer products differentiated in quality to consumers, not to relax downstream competition, but to improve their buyer power in the negotiation with their supplier. We consider a simple vertical industry where two producers sell products differentiated in quality to two retailers who operate in separated markets. In the game, first retailers choose which product to carry, then each retailer and her chosen producer bargain over the terms of a two-part tariff contract and retailers finally choose the quantities. When upstream production costs are convex, the share of the total profits going to the retailer would be higher if they choose to differentiate. We thus isolate the wish to differentiate as "only" due to...
Tipo: Working or Discussion Paper
Palavras-chave: Buyer Power; Product line; Differentiation; Marketing; L13; L42.
Ano: 2007
URL: http://purl.umn.edu/6866

Coffee Differentiation: Demand Analysis at Retail Level in the US Market
Provedor de dados: 31
Autores: Alamo,   Carmen I.; Malaga,   Jaime E.
Scanned data was used to estimate US coffee demand using an AIDS model. The estimated elasticities have the expected signs and magnitude. Differentiated coffees are complements for regular and unclassified while regular and unclassified coffees are substitutes. These results could be useful in designing marketing strategies by coffee suppliers.
Tipo: Presentation
Palavras-chave: Demand; Coffee; Differentiation; Demand and Price Analysis.
Ano: 2012
URL: http://purl.umn.edu/119885

Market Structure and Competition in Food Retail: Some Evidences from Brazil
Provedor de dados: 31
Autores: Monterio,   G.F.A.; Farina,   Elizabeth Maria Mercier Querido; Nunes,   R.
The paper analyzes competition among supermarkets in Brazil. In contrast to part of the economic literature which suggests that the fast growth of big supermarket chains would destroy independent, medium and small supermarkets, the paper argues that big supermarket chains can coexist with different formats of independent food retailing. As a result, competition in food retail is complex and cannot be described as a simple Darwinian process of market concentration. The analysis is divided in two parts. In the first part, the competition between hypermarkets and supermarkets is examined. Evidences for the district of Sao Paulo, Brazil, suggest that these retailers form separate markets. The second part is focused on neighborhood supermarkets. The results...
Tipo: Conference Paper or Presentation
Palavras-chave: Food retail; Supermarkets; Differentiation; Agribusiness; Industrial Organization.
Ano: 2008
URL: http://purl.umn.edu/44199

Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR
Provedor de dados: 89
Autores: Okino,CH; Montassier,MFSM; Givisiez,PEN; Furuyama,CRAG; Brentano,L; Montassier,HJ.
A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken embryos and from tissues of chickens experimentally infected with different strains of IBV. Amplification and identification of the viral RNA was performed using two sets of primers complementary to a region of the S glycoprotein gene in the Semi-N-RT-PCR assay. The pair of primers used in the first PCR consisted of universal oligonucleotides flanking a more variable region of S1-S2 gene. The second primer pair was used in the Semi-N-RT-PCR and was comprised of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detection; Differentiation; Infectious bronchitis virus; Semi-nested RT-PCR; Vaccine strain.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010

Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
Provedor de dados: 89
Autores: Paiva,JB; Cavallini,JS; Silva,MD; Almeida,MA; Ângela,HL; Berchieri Junior,A.
Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FliC gene; Differentiation; Salmonella Gallinarum; Salmonella Pullorum.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000400009

Roles of Hedgehog signaling pathway in adipogenic differentiation potential of porcine adipose-derived mesenchymal stem cells
Provedor de dados: 96
Autores: Fan,Caiyun; Zhang,Yunhai; Wang,Juhua; Cheng,Jianbo.
ABSTRACT This study examined the effects of purmorphamine and cyclopamine, classical agonists and inhibitors of the Hedgehog (Hh) signaling pathway, in the adipogenic differentiation of porcine adipose-derived mesenchymal stem cells (AMSC) to investigate the roles underlying adipogenic differentiation in AMSC. Porcine-derived AMSC were established, and the Hh signaling pathway was activated or inhibited by treatment with purmorphamine or cyclopamine. The adipogenic differentiation of the porcine AMSC was then analyzed by Oil Red O staining. The expression levels of Hh signaling pathway factors and adipogenic transcription factors were determined using quantitative real-time polymerase chain reaction and western blot analysis. We verified that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipogenesis; Differentiation; Expression; Pig.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100415

Consumer perception of Brazilian traced beef
Provedor de dados: 96
Autores: Barcellos,Júlio Otávio Jardim; Abicht,Alexandre de Melo; Brandão,Fernanda Scharnberg; Canozzi,Maria Eugênia Andrighetto; Collares,Fernando Carbonari.
The objective of this study was to determine consumers understanding of beef traceability, identifying how consumers value this meat and traceability elements to be presented on retail shelves. The method used in this study was a survey through the internet applying the Sphinx software. The sample consisted of 417 consumers, mostly living in Porto Alegre, Brazil. Consumers are aware of certified beef, consider it important, but this is not a demand. As to traced beef, most consumers (62.4%) are in favor of mandatory traceability of beef cattle in Brazil, but 86.6% disagree with the destination of traced beef only to the foreign market. The majority of people are willing to pay more for traced beef and consider traceability a market opportunity, used as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agribusiness; Certification; Differentiation; Quality; Traceability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300041

Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells
Provedor de dados: 42
Autores: Schwindt,Telma T.; Motta,Fabiana L.; Gabriela F.,Barnabé; Cristina G.,Massant; Guimarães,Alessander O.; Calcagnotto,Maria Elisa; Pesquero,João B.; Mello,Luiz E.
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neural precursor cells; Neurosphere; Fibroblast growth factor 2; Epidermal growth factor; Differentiation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009

In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review
Provedor de dados: 42
Autores: CECHIM,GIOVANA; CHIES,JOSÉ A.B.
Abstract: Dendritic cells (DCs) initiate and shape both innate and adaptive immune responses. They are specialized in antigen presentation to naive T cells, thereby orchestrating the T cell immune responses. Human peripheral blood and tissues contain several subsets of phenotypically and functionally distinct DCs, which promote interactions between the external environment and lymphoid organs. Because of the difficulty in purifying these cells, in vitro studies only became more frequent when Frederica Sallusto and Antonio Lanzavecchia developed a method to generate DCs from blood monocytes in vitro. Nowadays a wide range of biotechnological innovations has allowed the study of DCs and their precursors in the most diverse situations faced by the immune...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Dendritic cell; Differentiation; Monocytes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700614

Characterization of mesenchymal stem cells derived from equine adipose tissue
Provedor de dados: 45
Autores: Carvalho,A.M.; Yamada,A.L.M.; Golim,M.A.; Álvarez,L.E.C.; Jorge,L.L.; Conceição,M.L.; Deffune,E.; Hussni,C.A.; Alves,A.L.G.
Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunophenotypic characterization; Differentiation; Equine; Adipose tissue; Mesenchymal stem cell.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400001

Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation
Provedor de dados: 47
Autores: Julianelli,Vanina L; Guerra,Liliana N; Calvo,Juan C.
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triglycerides; Mammary epithelium; Differentiation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200006

Nkx-2.3 gene in mouse epidemial maturation
Provedor de dados: 48
Autores: MA,CHANG M.
In higher vertebrates, from amphibians to humans, epidemial maturation is a conserved developmental process. Using adult epidemial tissue and an established keratinocyte cell line, the mouse Nkx-2.3 homeobox gene was demonstrated, for the first time, to be expressed in mouse epidermal keratinocytes. Under the normal culture condition, the spontaneous aggregation phenomenon, a common initiation step of ES cell differentiation, and the induction of mouse adult K1 keratin, a marker of mature epidermal keratinocytes, were both observed in vitro when the Xenopus Nkx-2.3 gene was stably transfected into a mouse pluripotent P19 EC cell line. The induction of mouse K1 keratin by using its Xenopus orthologous gene in the mouse P19 cell implies that Nkx-2.3 may play...
Tipo: Journal article
Palavras-chave: Nkx-2.3; Epidermis; Homeobox gene; Skin maturation; Differentiation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300001

BJ-1108, a 6-Amino-2,4,5-trimethylpyridin-3-ol analogue, regulates differentiation of Th1 and Th17 cells to ameliorate experimental autoimmune encephalomyelitis
Provedor de dados: 48
Autores: Kang,Youra; Timilshina,Maheshwor; Nam,Tae-gyu; Jeong,Byeong-Seon; Chang,Jae-Hoon.
Abstract  Background  CD4+ T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-&amp;#947; (IFN-&amp;#947;)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases.  Results  In the current study, we found that BJ-1108, a 6-aminopyridin-3-ol analogue, significantly inhibited Th1 and Th17 differentiation in vitro in a concentration-dependent manner, with no effect on proliferation or apoptosis of activated T...
Tipo: Journal article
Palavras-chave: BJ-1108; Th1/Th17 cell; Differentiation; EAE.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100202

Early and late molecular and morphologic changes that occur during the in vitro transformation of Trypanosoma cruzi metacyclic trypomastigotes to amastigotes
Provedor de dados: 48
Autores: CONTRERAS,VÍCTOR T; NAVARRO,MARÍA C; DE LIMA,ANA R; DURAN,FRANCY; ARTEAGA,ROSA; FRANCO,YUNAIMY.
The amastigogenesis primary of T. cruzi occurs naturally when metacyclic trypomastigotes transform into amastigotes within the cells of the mammalian host. The in vitro study of the macromolecular changes that occur over several days during the transformation process should provide significant indications of how the parasite adapts to the mammalian host environment. We show here that metacyclic trypomastigotes pre-incubated at 37&amp;deg; C in a protein-rich medium reach a high degree of transformation to amastigotes when re-incubated in the fresh medium. Giemsa-stained smears show that during the pre-incubation phase, the metacyclic trypomastigotes undergo lengthening at the posterior end and a thinning out of the entire body. SDS-PAGE analysis of...
Tipo: Journal article
Palavras-chave: Amastigogenesis; Differentiation; Macromolecular changes; Metacyclic trypomastigotes; Trypanosoma cruzi.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100008

The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
Provedor de dados: 48
Autores: FELMER,RICARDO N; CLARK,JOHN A.
The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a...
Tipo: Journal article
Palavras-chave: Genetic ablation; NTR/CB1954; Adipocytes; Differentiation.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009

Nuclear translocation of STAT3 by in vitro metreleptin administration causes lipolysis in human primary adipocytes
Provedor de dados: 52
Autores: Choi,Seung Kug; Park,Sunmi; Moon,Hyun-Seuk.
We utilized subcutaneous (SC)- and omental (OM)-derived human primary adipocytes (hPA) from obese male, and investigated whether synthetic analog of leptin, metreleptin, may regulate lipolysis via translocation of STAT3 to the nucleus. We observed that 50 ng/mL of metreleptin increases STAT3 phosphorylation in both SC- and OM-derived hPA. Importantly, we found for the first time that metreleptin is capable of trans-locating STAT3 to the nucleus and STAT3 blockade inhibits metreleptin-induced lipolysis. Our initial data provide novel insights into the role of STAT3 as probable mediator of the action of metreleptin in regulating metabolism.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metreleptin; Human primary adipocyte; STAT3; Differentiation; Lipolysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100309

Chromosomal differentiation between populations of Oligosarcus hepsetus (Teleostei, Characidae) from small tributaries at opposite margins of the Paraíba do Sul river (Brazil)
Provedor de dados: 52
Autores: Centofante,Liano; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Cytogenetic analyses were carried out in two populations of Oligosarcus hepsetus from tributaries at opposite margins of the Paraíba do Sul river. The same diploid number was observed in both populations (2n=50), but they showed remarkable differences related to karyotype formula and distribution of rDNA sites as revealed by silver nitrate staining and in situ hybridization with 18S probes. The results suggested that the main channel of the Paraíba do Sul river acted as barrier to gene flow between populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Headstream; Cytogenetic; Differentiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700016

Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species
Provedor de dados: 52
Autores: Kumar,Ashwani; Goyal,Suresh Chand; Dhansu,Pooja; Sheoran,Parvender; Kumar,Arvind; Verma,Ekta; Parshad,Jagdish; Singh,Anshuman.
ABSTRACTThe present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemented with NAA (2.5 mg/L) inDioscorea alata and 2, 4-D (2.0 mg/L) inD. deltoidea, respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea alata; Dioscorea deltoidea; Callus; Differentiation; Polypeptides.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500667

In vitro proliferation and differentiation of hepatic oval cells and their potential capacity for intrahepatic transplantation
Provedor de dados: 56
Autores: Li,Z.; Chen,J.; Li,L.; Ran,J.H.; Liu,J.; Gao,T.X.; Guo,B.Y.; Li,X.H.; Liu,Z.H.; Liu,G.J.; Gao,Y.C.; Zhang,X.L.
Hepatic oval cells (HOCs) are recognized as facultative liver progenitor cells that play a role in liver regeneration after acute liver injury. Here, we investigated the in vitro proliferation and differentiation characteristics of HOCs in order to explore their potential capacity for intrahepatic transplantation. Clusters or scattered HOCs were detected in the portal area and interlobular bile duct in the liver of rats subjected to the modified 2-acetylaminofluorene and partial hepatectomy method. Isolated HOCs were positive for c-kit and CD90 staining (99.8% and 88.8%, respectively), and negative for CD34 staining (3.6%) as shown by immunostaining and flow cytometric analysis. In addition, HOCs could be differentiated into hepatocytes and bile duct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic oval cells; Proliferation; Differentiation; Liver transplantation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800681

Tissue transglutaminase (TG2) activity regulates osteoblast differentiation and mineralization in the SAOS-2 cell line
Provedor de dados: 56
Autores: Yin,Xiaoxue; Chen,Zhongqiang; Liu,Zhongjun; Song,Chunli.
Tissue transglutaminase (type II, TG2) has long been postulated to directly promote skeletal matrix calcification and play an important role in ossification. However, limited information is available on the expression, function and modulating mechanism of TG2 during osteoblast differentiation and mineralization. To address these issues, we cultured the well-established human osteosarcoma cell line SAOS-2 with osteo-inductive conditioned medium and set up three time points (culture days 4, 7, and 14) to represent different stages of SAOS-2 differentiation. Osteoblast markers, mineralization, as well as TG2 expression and activity, were then assayed in each stage. Furthermore, we inhibited TG activity with cystamine and then checked SAOS-2 differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transglutaminase; Osteoblast; Differentiation; Mineralization; Bone morphogenetic protein-2 (BMP-2).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800002

Thyroid hormone regulates protein expression in C6 glioma cells
Provedor de dados: 56
Autores: Trentin,A.G.; Alvarez-Silva,M.
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008

Retinoic acid and cAMP inhibit rat hepatocellular carcinoma cell proliferation and enhance cell differentiation
Provedor de dados: 56
Autores: Ionta,M.; Rosa,M.C.; Almeida,R.B.; Freitas,V.M.; Rezende-Teixeira,P.; Machado-Santelli,G.M.
Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion; CAMP; Differentiation; Proliferation; Hepatocarcinoma; Retinoic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800006

Serotonin regulates osteoblast proliferation and function in vitro
Provedor de dados: 56
Autores: Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759

Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line
Provedor de dados: 56
Autores: Li,Ying-fei; Xiao,Bing; Tu,San-fang; Wang,Yuan-yuan; Zhang,Xiao-lang.
Our group established a method to culture spheres under serum-free culture condition. However, the biological characteristics and the tumorigenicity of spheres are unknown. Here, we demonstrate that sphere cells expressed high levels of the putative colorectal cancer stem cell markers CD133 and CD44. The CD133-positive rates were 13.27 ± 5.62, 52.71 ± 16.97 and 16.47 ± 2.45% in sphere cells, regular Colo205 cells and differentiated sphere cells, respectively, while the CD44-positive rates were 62.92 ± 8.38, 79.06 ± 12.10 and 47.80 ± 2.5%, respectively, and the CD133/CD44-double-positive rates were 10.77 ± 4.96, 46.89 ± 19.17 and 12.41 ± 2.27%, respectively (P &lt; 0.05). Cancer sphere cells formed crypt-like structures in 3-D culture. Moreover, cells from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colon cancer stem cells; Cancer sphere; Self-renew; Differentiation; Tumorigenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300003

Tetramethylpyrazine potentiates arsenic trioxide activity against HL-60 cell lines
Provedor de dados: 56
Autores: Wu,Yuni; Xu,Youhua; Shen,Yali; Wang,Cuicui; Guo,Gaili; Hu,Tiantian.
The objective of this study was to evaluate the effects of tetramethylpyrazine (TMP) in combination with arsenic trioxide (As2O3) on the proliferation and differentiation of HL-60 cells. The HL-60 cells were treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively. The proliferative inhibition rates were determined with MTT. Differentiation was detected by the nitroblue tetrazolium (NBT) reduction test, Wright’s staining and the distribution of CD11b and CD14. Flow cytometry was used to analyze cell cycle distribution. RT-PCR and Western blot assays were employed to detect the expressions of c-myc, p27, CDK2, and cyclin E1. Combination treatment had synergistic effects on the proliferative inhibition rates. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetramethylpyrazine; Acute promyelocytic leukemia; Arsenic trioxide; HL-60 cell; Proliferation; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300002

Biology and clinical utilization of mesenchymal progenitor cells
Provedor de dados: 56
Autores: Minguell,J.J.; Conget,P.; Erices,A.
Within the complex cellular arrangement found in the bone marrow stroma there exists a subset of nonhematopoietic cells referred to as mesenchymal progenitor cells (MPC). These cells can be expanded ex vivo and induced, either in vitro or in vivo, to terminally differentiate into at least seven types of cells: osteocytes, chondrocytes, adipocytes, tenocytes, myotubes, astrocytes and hematopoietic-supporting stroma. This broad multipotentiality, the feasibility to obtain MPC from bone marrow, cord and peripheral blood and their transplantability support the impact that the use of MPC will have in clinical settings. However, a number of fundamental questions about the cellular and molecular biology of MPC still need to be resolved before these cells can be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal progenitor cells; Transplantation; Differentiation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800003

Intramyocardial implantation of differentiated rat bone marrow mesenchymal stem cells enhanced by TGF-β1 improves cardiac function in heart failure rats
Provedor de dados: 56
Autores: Lv,Y.; Liu,B.; Wang,H.P.; Zhang,L.
The present study tested the hypotheses that i) transforming growth factor beta 1 (TGF-β1) enhances differentiation of rat bone marrow mesenchymal stem cells (MSCs) towards the cardiomyogenic phenotype and ii) intramyocardial implantation of the TGF-β1-treated MSCs improves cardiac function in heart failure rats. MSCs were treated with different concentrations of TGF-β1 for 72 h, and then morphological characteristics, surface antigens and mRNA expression of several transcription factors were assessed. Intramyocardial implantation of these TGF-β1-treated MSCs to infarcted heart was also investigated. MSCs were initially spindle-shaped with irregular processes. On day 28 after TGF-β1 treatment, MSCs showed fusiform shape, orientating parallel with one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone marrow mesenchymal stem cell; Transforming growth factor beta1; Cardiomyocytes; Differentiation; Intramyocardial implantation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600603

Sec61alpha synthesis is enhanced during translocation of nascent chains of collagen type IV in F9 teratocarcinoma cells after retinoic acid treatment
Provedor de dados: 56
Autores: Ferreira,L.R.; Velano,C.E.E.; Braga,E.C.; Paula,C.C.; Martéli Junior,H.; Sauk,J.J.
Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (ER) membrane through a protein-conducting channel called translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as being essential for translocation of polypeptide chains into the cisterns of the ER. Sec61alpha forms a protein complex with collagen and Hsp47, an ER-resident heat shock protein that binds specifically to collagen. However, it is not known whether Sec61alpha is ubiquitously produced in collagen-producing F9 teratocarcinoma cells or under heat shock treatment. Furthermore, the production and utilization of Sec61alpha may depend on the stage of cell differentiation. Cultured F9 teratocarcinoma cells are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sec61alpha; Collagen type IV; Retinoic acid; F9 cells; Differentiation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100005

PD-1/PD-L1 regulates Treg differentiation in pregnancy-induced hypertension
Provedor de dados: 56
Autores: Jiang,Lai; Tang,Chaoliang; Gong,Yanping; Liu,Yujie; Rao,Jie; Chen,Suyu; Qu,Wanjun; Wu,Dabao; Lei,Lei; Chen,Ling.
Pregnancy-induced hypertension (PIH) causes significant maternal and fetal morbidity and mortality. A decreased number of regulatory T (Treg) cells is associated with the pathogenesis of PIH. The programmed cell death-1 (PD-1)/PD-ligand 1 (PD-L1) pathway is critical to normal pregnancy (NP) by promoting Treg cell development. However, the relationship between PD-1/PD-L1 and Treg differentiation in PIH has not been fully elucidated. In this study, venous blood was obtained from 20 NP and 58 PIH patients. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood. The levels of Treg-related cytokines (TGF-β, IL-10, and IL-35) in serum and PBMCs were measured by ELISA. The percentage of Treg cells in PBMCs was assessed by flow cytometry. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PD-1/PD-L1; Treg; PIH; Foxp3; Differentiation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800605

Rhinovirus detection using different PCR-based strategies
Provedor de dados: 58
Autores: Silva,E.R.M.; Watanabe,A.S.A.; Carraro,E.; Perosa,A.H.S.; Granato,C.F.H.; Bellei,N.C.J.
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinovirus; Enterovirus; Detection; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200038

Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and Salmonella enterica subsp enterica serovar Gallinarum
Provedor de dados: 58
Autores: Ribeiro,Simone Alves Mendes; Paiva,Jaqueline Boldrin de; Zotesso,Fábio; Lemos,Manoel Victor Franco; Berchieri Júnior,Ângelo.
S. Pullorum (SP) and S. Gallinarum (SG) are very similar. They are the agents of pullorum disease and fowl typhoid, respectively, and the two diseases are responsible for economic losses in poultry production. Although SP and SG are difficult to be differentiated in routine laboratory procedures, the ability to metabolize ornithine is a biochemical test that may be used to achieve this aim. While SP is able to decarboxylate this amino acid, SG is not. However, the isolation of strains showing atypical biochemical behavior has made this differentiation difficult. One of the genes associated with the metabolization of the amino acid ornithine is called speC, and is found in both serovars. The analysis of 21 SP and 15 SG strains by means of PCR did not enable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella Pullorum; Salmonella Gallinarum; Differentiation; PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100032

A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis
Provedor de dados: 58
Autores: Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da.
Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004

Beef Consumer Preferences in Chile: Importance of Quality Attribute Differentiators on the Purchase Decision
Provedor de dados: 117
Autores: Villalobos,Pablo; Padilla,Carlos; Ponce,Cristian; Rojas,Álvaro.
Agrifood markets worldwide have focused on searching for new quality attribute differentiators, which capture the attention of consumers and meet their needs. The purpose of this research was to determine the importance of a set of quality attribute differentiators associated with a beef cut on the choice behavior of the Chilean consumer. The evaluated differentiating characteristics were: price, origin, production method, and quality assurance. A total of 750 subjects were surveyed in the following cities: Talca, Rancagua, and Santiago. Conjoint analysis was carried out to estimate the impact of the assessed attributes on the purchase decision of the consumers polled. Findings point out that the quality attribute differentiators significantly influence...
Tipo: Journal article
Palavras-chave: Conjoint analysis; Country of origin; Animal welfare; Quality assurance; Differentiation; Marketing.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000100009

Differentiation of sheep milk and cheese based on quality and composition
Provedor de dados: 118
Autores: Vera,Raúl R; Aguilar,Claudio; Lira,Rodrigo.
Traditional sheep production for meat and wool for meat and wool among small and medium-sized Chilean farmers has low profitability. Therefore, there is interest in producing value-added, differentiated products. One alternative is the production of sheep milk and cheese. This arricie analyzes and discusses existing alternatives for modifying milk and cheese compositions with the aim of differentiating these products. Also, analytical techniques that allowthe chemical characterization of milk and cheese are briefly mentioned. The main international thrusts are focused on the modification of the content and composition of milk fatty acids and on the identification of volatile compounds, terpenes, polyphenols and other analytes that allow differentiation of...
Tipo: Journal article
Palavras-chave: Cheese; Differentiation; Flavor; Milk composition sheep value-added.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000300001