Consulta: subjectFacets:"Dinophysistoxins"
Registros recuperados: 2
Data/hora: 01/06/2024 10:23:48

Contribution to toxicity assessment of Dinophysis acuminata (Dinophyceae)
Provedor de dados: 5
Autores: Marcaillou-le Baut, Claire; Mondeguer, Florence; Gentien, Patrick.
Blooms of Dinophysis in French coastal waters are implicated in most bans on marketing commercial bivalves. However, the relation between Dinophysis cell density and shellfish toxicity is not always consistent. Discrepancies may be due to the simple fact that it is nearly impossible to compare an integral over a few days (shellfish toxin content) and water samples. Furthermore, it seems that cells may have a variable specific toxicity. This work focuses on the variability in cell toxicity taking into account recent findings and using liquid chromatography coupled to mass spectrometry with an ion trap and electrospray interface. Esterified analogues of okadaic acid (DTX-4 and diol-esters) have been identified in cultures of Prorocentrum lima, another...
Tipo: Text
Palavras-chave: Liquid chromatography electrospray ionization mass spectrometry; Okadaic acid; Dinophysistoxins; Dinophysis acuminata.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-605.pdf

Validation d’un procédé automatisé d’extraction sur phase solide de glandes digestives de moules pour l’identification et la quantification des dinophysistoxines en LC/ESI/SM2 par piégeage d’ions quadripolaire
Provedor de dados: 5
Autores: Mondeguer, Florence; Mestres, Gilberte; Rouland, Caroline; Montagu, Monique; Maillols, Hélène.
This report presents a new extraction method of the dinophysistoxins (DTXs), confirmed by quantification using high-performance liquid chromatography coupled to mass spectrometry with an ion trap and electro spray interface (HPLC/ESI/MS2). The method originality consists on the adaptation of DTXs basic extraction procedure (liquid/ liquid) to a solid phase extraction (SPE) via a robotic station: ASPEC XLi The parameters of the automatization procedure were optimized to obtain the best DTXs recovery rate. These improvements were loaded with digestive gland mussel homogenat realized on a silica cartridge SPE, activated in hexane/chloroform (50:50), washed with hexane/chloroform (50:50) and extracted by an elution gradient (chloroform methanol (65:35) and...
Tipo: Text
Palavras-chave: Toxines; Intoxication des coquillages; Dinophysistoxines; Acide okadaïque; Dinophysistoxine-1; Méthode alternative; Extraction sur phase solide; Automatisation; Couplage CLHP/ESI/SM2 par piège d’ions.; Toxins; Shellfish poisoning; Dinophysistoxins; Okadaic acid; Dinophysistoxin-1; Alternative method; Solid phase extraction; Automation; Liquid chromatography coupled ion trap.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00342/45270/44724.pdf