Consulta: subjectFacets:"EST"
Registros recuperados: 23
Data/hora: 06/06/2024 11:29:27

Suppression subtractive hybridization PCR isolation of cDNAs from a Caribbean soft coral
Provedor de dados: 69
Autores: Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr.
Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-&amp;#8220;housekeeping&amp;#8221; genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila...
Tipo: Journal article
Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos
Provedor de dados: 69
Autores: Li,Ai Qin; Zhao,Chuan Zhi; Wang,Xing Jun; Liu,Zhan Ji; Zhang,Li Feng; Song,Guo Qi; Yin,Juan; Li,Chang Sheng; Xia,Han; Bi,Yu Ping.
Microsatellites, or simple sequence repeats (SSRs), in expressed sequence tags (ESTs) provide an opportunity for low cost SSR development. We looked for EST-SSRs in 403,511 ESTs (generated by 454 sequencing and representing 70,654 contigs and 52,082 singletons) from soybean globular stage embryos. Among 122,736 unique ESTs, 3,729 contained one or more SSRs. In total, 3,989 SSRs were identified including 304 mono, 1,374 di, 2,208 tri, 70 tetra, 13 penta and 20 hexanucleotide SSRs. Thirty three EST-SSRs were selected for primer design and polymorphism analysis using twenty soybean cultivars and one wild-type soybean. Successful amplification was obtained using 21 of these primer pairs, 11 of which detected polymorphisms in these soybean cultivars. These...
Tipo: Journal article
Palavras-chave: 454 sequencing; EST; Glycine max; Polymorphism; SSR.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500006

A transcriptome analysis of mitten crab testes (Eriocheir sinensis)
Provedor de dados: 74
Autores: Zhang,Wei; Wan,Haolei; Jiang,Hui; Zhao,Yunlong; Zhang,Xiaowei; Hu,Songnian; Wang,Qun.
The identification of expressed genes involved in sexual precocity of the mitten crab (Eriocheir sinensis) is critical for a better understanding of its reproductive development. To this end, we constructed a cDNA library from the rapid developmental stage of testis of E. sinensis and sequenced 3,388 randomly picked clones. After processing, 2,990 high-quality expressed sequence tags (ESTs) were clustered into 2,415 unigenes including 307 contigs and 2,108 singlets, which were then compared to the NCBI non-redundant (nr) protein and nucleotide (nt) database for annotation with Blastx and Blastn, respectively. After further analysis, 922 unigenes were obtained with concrete annotations and 30 unigenes were found to have functions possibly related to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproduction; Testis; EST; Eriocheir sinensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100024

Tandem repeat distribution of gene transcripts in three plant families
Provedor de dados: 74
Autores: Maia,Luciano Carlos da; Souza,Velci Queiróz de; Kopp,Mauricio Marini; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; EST; Comparative genomics; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400025

Expression profile of oxidative and antioxidative stress enzymes based on ESTs approach of citrus
Provedor de dados: 74
Autores: Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth.
Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016

Analysis of expressed sequence tags from Citrus sinensis L. Osbeck infected with Xylella fastidiosa
Provedor de dados: 74
Autores: Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A.
In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sweet orange; EST; CVC; Compatible interaction.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023

Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction
Provedor de dados: 74
Autores: Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A.
Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026

Exploiting a wheat EST database to assess genetic diversity
Provedor de dados: 74
Autores: Karakas,Ozge; Gurel,Filiz; Uncuoglu,Ahu Altinkut.
Expressed sequence tag (EST) markers have been used to assess variety and genetic diversity in wheat (Triticum aestivum). In this study, 1549 ESTs from wheat infested with yellow rust were used to examine the genetic diversity of six susceptible and resistant wheat cultivars. The aim of using these cultivars was to improve the competitiveness of public wheat breeding programs through the intensive use of modern, particularly marker-assisted, selection technologies. The F2 individuals derived from cultivar crosses were screened for resistance to yellow rust at the seedling stage in greenhouses and adult stage in the field to identify DNA markers genetically linked to resistance. Five hundred and sixty ESTs were assembled into 136 contigs and 989 singletons....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; EST; Genetic diversity; Triticum; Yellow rust.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400019

ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus
Provedor de dados: 74
Autores: Cassoli,Clarissa Sanches da Silva; Jorge,Erika Cirstina; Patrício,Mateus; Alves,Helena Javiel; Almeida,Erik Amazonas de; Ledur,Mônica Corrêa; Coutinho,Luiz Lehmann.
Brazilian poultry industry has reached a high level of development in both meat and egg production as a result of constant technological modernization. Further improvements can be achieved through genomics, but before this can be accomplished, a better understanding of gene expression profiles and nucleotide polymorphisms is necessary. Since animal physiology is directly or indirectly controlled by the pituitary and hypothalamus, the aim of the present work was to identify and analyze genes expressed in these tissues in chicken lines with different growth potential. Two pituitary and hypothalamus cDNA libraries from 21 day broiler (TT) and layer (CC) chickens lines were constructed and allowed identification of 3,074 unique sequences and 77 single...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary; Hypothalamus; EST; SNP; Chicken.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600008

In silico prediction of gene expression patterns in Citrus flavedo
Provedor de dados: 74
Autores: Berger,Irving J.; Freitas-Astúa,Juliana; Reis,Marcelo S.; Targon,Maria Luísa P.N.; Machado,Marcos A.
Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Fruit; Gene expression; Orange; Tissue-specific.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500004

Terpene production in the peel of sweet orange fruits
Provedor de dados: 74
Autores: Takita,Marco A.; Berger,Irving J.; Basílio-Palmieri,Ana Carolina; Borges,Kleber M.; Souza,Juliana M. de; Targon,Maria L.N.P.
Terpenoids constitute the largest and most diverse class of natural products. They are important factors for aroma and flavor, and their synthesis is basically done from two compounds: isopentenyl diphosphate and dimethylallyl diphosphate. Isopentenyl diphosphate is synthesized through two different pathways, one that occurs in the cytoplasm and one in the plastid. With the sequencing of ESTs from citrus, we were able to perform in silico analyses on the pathways that lead to the synthesis of terpenes as well as on the terpene synthases present in sweet orange. Moreover, expression analysis using real-time qPCR was performed to verify the expression pattern of a terpene synthase in plants. The results show that all the pathways for isopentenyl diphosphate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; Fruit; Terpenoids; Orange; Essential oil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500012

The post-transcriptional gene silencing pathway in Eucalyptus
Provedor de dados: 74
Autores: Sassaki,Flávio Tetsuo; Campos-Pereira,Tiago; Maia,Ivan de Godoy.
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene silencing; PTGS; RNA silencing; EST; Eucalyptus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400003

In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations
Provedor de dados: 74
Autores: Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui.
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013

Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)
Provedor de dados: 74
Autores: Preechaphol,Rachanimuk; Klinbunga,Sirawut; Khamnamtongand,Bavornlak; Menasveta,Piamsak.
Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previously deposited in GenBank. Several reproduction-related transcripts were identified. The full-length cDNA of anaphase promoting complex subunit 11 (PmAPC11; 600 bp with an ORF of 255 bp corresponding to a polypeptide of 84 amino acids) and selenoprotein M precursor (PmSePM; 904 bp with an ORF of 396 bp corresponding to a polypeptide of 131 amino acids) were characterized and reported for the first time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EST; SSH; Penaeus monodon; Ovarian development; Semiquantitative RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400014

ESTs and putative line-specific (broiler and layer) SNPs identified in genes expressed in Gallus gallus pituitary and hypothalamus.
Provedor de dados: 14
Autores: CASSOLI, C. S. da S.; JORGE, E. C.; PATRÍCIO, M.; ALVES, H. J.; ALMEIDA, E. A. de; LEDUR, M. C.; COUTINHO, L. L.
bitstream/item/139799/1/digitalizar0003.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Hipotálamo; Glândula pituitária; EST; SNP.; Frango.; Expressed sequence tags.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/443641

Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment
Provedor de dados: 5
Autores: Li, Ronghua; Li, Qi; Cornette, Florence; Degremont, Lionel; Lapegue, Sylvie.
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously...
Tipo: Text
Palavras-chave: Pacific oyster; Crassostrea gigas; Microsatellite; EST; Multiplex PCR; Parentage assignment.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12419/9999.pdf

Crassostrea gigas ferritin: cDNA sequence analysis for two heavy chain type subunits and protein purification
Provedor de dados: 5
Autores: Durand, Jean-pierre; Goudard, Françoise; Pieri, Jacques; Escoubas, Jean-michel; Schreiber, Nathalie; Cadoret, Jean-paul.
Ferritin has been shown as being the principal iron storage in the majority of living organisms. In marine species, ferritin is also involved in high-level accumulation of Po-210. As part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. Ferritin was purified from the visceral mass of the oyster Crassostrea gigas by ion-exchange chromatography and HPLC. SDS-PAGE revealed one band of 20 kDa. An Expressed Sequence Tag (EST) library was screened and led to the identification of two complementary DNA (cDNA) involved in ferritin subunit expression. The complete coding sequences and the untranslated regions (UTRs) of the two genes were obtained and a 5' Rapid...
Tipo: Text
Palavras-chave: Iron; IRE; EST; Oyster.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-693.pdf

Expressed sequence tags in venomous tissue of Scorpaena plumieri (Scorpaeniformes: Scorpaenidae)
Provedor de dados: 83
Autores: Costa,Fábio L. S.; Lima,Maria E. De; Pimenta,Adriano C.; Figueiredo,Suely G.; Kalapothakis,Evanguedes; Salas,Carlos E.
Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNAs; EST; Glands; Lectin; Scorpionfish; Toxins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400871

ETIQUETAS DE SECUENCIAS EXPRESADAS DIFERENCIALES DE FRUTOS DE AGUACATE RAZA MEXICANA (Persea americana Mill. var. drymifolia)
Provedor de dados: 94
Autores: Sánchez-González,Enrique Ignacio; Hernández-Delgado,Sanjuana; Aguirre-Arzola,Víctor Eustorgio; Torres-Castillo,Jorge Ariel; Gutiérrez-Díez,Adriana.
RESUMEN  En este estudio se desarrollaron e identificaron etiquetas de secuencias expresadas diferencialmente (ESTs) de frutos inmaduros de Persea americana Mill. var. drymifolia del estado de Nuevo León, México. Diez genotipos con frutos de forma y tamaño diferentes fueron seleccionados para generar ESTs por la técnica de despliegue diferencial.Se obtuvieron en total 393 fragmentos diferenciales amplificados, 82 fragmentos diferenciales fueron secuenciados y editados para identificación y comparación en las bases de datos para nucleótidos y proteínas del NCBI. Cuarenta secuencias mostraron similitud significativa con secuencias de ARNm y/o secuencias de proteínas hipotéticas o predichas pertenecientes a P. americana y/o a otros géneros. Algunas secuencias...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aguacate; Raza Mexicana; Despliegue diferencial; EST.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100102

Caracterização isoenzimática e morfológica de clones e introduções de alho
Provedor de dados: 53
Autores: Siqueira,Walter José; Medina Filho,Herculano Penna; Lisbão,Rogério Salles; Fornasier,João Baptista.
Em virtude do grande número de denominações locais para clones de alho, nem sempre correspondentes a materiais distintos, conduziu-se o presente estudo objetivando a caracterização e classificação de 72 clones e introduções de alho (Allium sativum L.), e um clone de alho-rei (A. ampeloprasum L.). Isso foi feito analisando as isoenzimas alcooldesidrogenase (ADH), esterase (EST), peroxidase (PRX) e fosfoglucoisomerase (PGI) através da técnica de eletroforese horizontal em gel de amido hidrolisado de batata. Verificou-se que os clones nacionais e introduzidos se enquadram nos grupos aqui denominados DIKA ou CJLB, respectivamente para os padrões de ADH, EST, PRX e PGI. Entretanto, os padrões CILB, CJKB e CIKB foram observados em alguns clones estrangeiros,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eletroforese; Isoenzimas ADH; EST; PRX; PGI; Alho; Caracterização de cultivares.
Ano: 1985
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051985000100032

Identifying Eucalyptus expressed sequence tags related to Arabidopsis flowering-time pathway genes
Provedor de dados: 61
Autores: Dornelas,Marcelo Carnier; Rodriguez,Adriana Pinheiro Martinelli.
Flowering initiation depends on the balanced expression of a complex network of genes that is regulated by both endogenous and environmental factors. The timing of the initiation of flowering is crucial for the reproductive success of plants; therefore, they have developed conserved molecular mechanisms to integrate both environmental and endogenous cues to regulate flowering time precisely. Extensive advances in plant biology are possible now that the complete genome sequences of flowering plants is available and plant genomes can be comprehensively compared. Thus, association studies are emerging as powerful tools for the functional identification of genes involved on the regulation of flowering pathways. In this paper we report the results of our search...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CONSTANS; Data mining; EST; Photoperiod; Vernalization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200009

Brazilian coffee genome project: an EST-based genomic resource
Provedor de dados: 61
Autores: Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães.
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; CDNA; EST; Transcriptome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008

Cytology, biochemistry and molecular changes during coffee fruit development
Provedor de dados: 61
Autores: De Castro,Renato D.; Marraccini,Pierre.
In commercial coffee species (Coffea arabica and Coffea canephora), fruit development is a lengthy process, characterized by tissue changes and evolutions. For example, soon after fecundation and up to mid development, the fruit is mainly constituted of the pericarp and perisperm tissue. Thereafter, the perisperm gradually disappears and is progressively replaced by the endosperm (true seed). Initially present in a "liquid" state, the endosperm hardens as it ripens during the maturation phase, as a result of accumulation of storage proteins, sucrose and complex polysaccharides representing the main reserves of the seed. The last step of maturation is characterized by the dehydration of the endosperm and the color change of the pericarp. Important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea spp; Bean development; Cell cycle; Endosperm; EST; Gene expression; Pericarp; Perisperm.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100013