Consulta: subjectFacets:"Endoxylanase"
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Data/hora: 01/06/2024 11:21:25

Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03
Provedor de dados: 58
Autores: Dobrev,Georgi Todorov; Zhekova,Boriana Yordanova.
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endoglucanase; Purification; Characterization; Aspergillus niger; Endoxylanase.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008