Consulta: subjectFacets:"Enteric viruses"
Registros recuperados: 6
Data/hora: 07/06/2024 11:19:05

Validity of Escherichia coli, enterovirus, and F-specific RNA bacteriophages as indicators of viral shellfish contamination
Provedor de dados: 5
Autores: Miossec, Laurence; Le Guyader, Soizick; Pelletier, Dominique; Haugarreau, Larissa; Caprais, Marie-paule; Pommepuy, Monique.
The sanitary classification of harvesting areas for bivalve mollusks in France is based on the level of Escherichia Coli contamination detected in shellfish meat, as defined in EC Directive 91/492 EEC. However, outbreaks of gastroenteritis or hepatitis after consumption of shellfish meeting current bacteriological standards suggest that E. coli is a poor indicator of viral contamination. The purpose of this study was to assess the adequacy of enterovirus and F-specific RNA bacteriophages as new indicators of human enteric viruses. Shellfish were sampled over a 37-mo period to characterize microbial contamination in two coastal areas subjected to different sewage contamination inputs. Contamination by E. coli, F-specific RNA bacteriophages (F+ RNA) and...
Tipo: Text
Palavras-chave: Indicator; Escherichia coli; F specific RNA bacteriophages; Enteric viruses; Viral contamination; Shellfish.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/00000/10006/7794.pdf

Devenir des virus entériques en mer et influence des facteurs environnementaux
Provedor de dados: 5
Autores: Gantzer, Christophe; Dubois, Éric; Crance, Jean-marc; Billaudel, Sylviane; Kopecka, Helena; Schwartzbrod, Louis; Pommepuy, Monique; Le Guyader, Françoise.
To improve the knowledge of the survival of enteric viruses in a marine environment, the influence of physico-chemical parameters (temperature, UV, salinity) on the survival of infectious poliovirus 1 and hepatitis A Virus (HAV) in seawater was first studied, the influence of suspended solids (SS) on poliovirus adsorption and survival in seawater was then evaluated and the detection of rotavirus genome in environmental samples (shellfish, river water, treated wastewater) was finally investigated. The results show that temperature has a major impact on virus survival in seawater as the time necessary to inactivate 90 % of the virus (T-90) is 671 days at 4 degrees C and only 25 days at 25 degrees C. Ultraviolet light (42 mW s cm(-2)) rapidly inactivates...
Tipo: Text
Palavras-chave: Environmental factors; Survival; Seawater; Enteric viruses; Facteurs environnementaux; Survie; Eau de mer; Virus entériques.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/publication-846.pdf

Analytical Methods for Virus Detection in Water and Food
Provedor de dados: 5
Autores: Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane.
Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses...
Tipo: Text
Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf

Assessment of human enteric viruses in cultured and wild bivalve molluscs
Provedor de dados: 5
Autores: Luz Vilarino, M.; Le Guyader, Soizick; Polo, David; Schaeffer, Julien; Krol, Joanna; Romalde, Jesus L.
Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ria de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from Cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated...
Tipo: Text
Palavras-chave: Seafood industry; Viral quantification; Viral prevalence; Norovirus; Hepatitis A virus; Enteric viruses; Molluscs.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6957.pdf

Rotavirus detection and isolation from chickens with or without symptoms
Provedor de dados: 89
Autores: Villarreal,LYB; Uliana,G; Valenzuela,C; Chacón,JLV; Saidenberg,ABS; Sanches,AA; Brandão,PE; Jerez,JA; Ferreira,AJP.
Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteric viruses; Rotavirus; Isolation; MA104 cells; Chicken.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300009

Detection of enteric viruses in activated sludge by feasible concentration methods
Provedor de dados: 58
Autores: Prado,Tatiana; Gaspar,Ana Maria Coimbra; Miagostovich,Marize Pereira.
Human enteric viruses are responsible to cause several diseases, including gastroenteritis and hepatitis, and can be present in high amounts in sewage sludge. This study compared virus recovery efficiency of two feasible concentration methods used for detecting human adenovirus (HAdV), rotavirus species A (RV-A), norovirus genogroup II (NoV GII) and hepatitis A virus (HAV) in sewage sludge from an activated sludge process. Twelve sewage sludge samples were collected bi-monthly from January to July, 2011. Ultracentrifugation was compared with a simplified protocol based on beef extract elution for recovering enteric viruses. Viruses were quantified by quantitative real-time PCR assays and virus recovery efficiency and limits of detection were determined....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enteric viruses; Sewage sludge; Activated sludge process; Virus concentration methods.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100049