Consulta: subjectFacets:"Enterobacteriaceae"
Registros recuperados: 51
Data/hora: 16/06/2024 03:48:48

Quantitative analysis of the intestinal bacterial communities in broiler chickens using qPCR and metagenomic analysis.
Provedor de dados: 119
Autores: FURLAN, L.; CANTÃO, M.; GIACHETTO, P.; LUNEDO, R.; FERNANDES, C.; MACARI, M.
The 16S rDNA sequence analysis has been one of the most used approaches for microbiota taxonomic profiling. Among the available techniques, real-time quantitative PCR (qPCR) and next generation sequencing (NGS) and metagenomic analysis has been widely used by researchers, because in addition to qualitative, it also provide quantitative data. Thus, the aim of our study was to compare the effectiveness of these 2 methods for quantification of 3 groups of bacteria of the intestinal microbiota of broilers fed on a standard corn-soybean based diet.
Tipo: Separatas
Palavras-chave: Análise de DNA; Análise metagenômica.; Enterobacteriaceae; Metagenomics.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/953791

Quantitative analysis of the intestinal bacterial communities in broiler chickens using qPCR and metagenomic analysis.
Provedor de dados: 119
Autores: FURLAN, L.; CANTÃO, M.; GIACHETTO, P.; LUNEDO, R.; FERNANDES, C.; MACARI, M.
The 16S rDNA sequence analysis has been one of the most used approaches for microbiota taxonomic profiling. Among the available techniques, real-time quantitative PCR (qPCR) and next generation sequencing (NGS) and metagenomic analysis has been widely used by researchers, because in addition to qualitative, it also provide quantitative data. Thus, the aim of our study was to compare the effectiveness of these 2 methods for quantification of 3 groups of bacteria of the intestinal microbiota of broilers fed on a standard corn-soybean based diet.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Análise de DNA; Análise metagenômica; Metagenomics; Enterobacteriaceae.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946489

Microbiological dynamics of Piatã grass silage (U. brizantha cv. BRS Piatã) with additives.
Provedor de dados: 119
Autores: SEDANO, A. D. B.; PEREIRA, D. H.; PEDREIRA, B. C. e; PINA, D. S.; BOURSCHEIDT, M. L. B.; MOMBACH, M. A.; XAVIER, I. M.; SCHIMTED JUNIOR, R. J.
One of the alternatives to improve the ensilage process of tropical climate grasses is the use of bacterial and bacterial additives, which provide microorganisms desirable for fermentation, in addition to inhibiting the growth of undesirable microorganisms, which guarantees the formation of products that do not inhibit consumption and livestock production. The objective of this study was to evaluate the effect of the addition of additives on the microbial population of Urochloa brizantha cv. BRS Piatã. The piatã grass was established in the experimental area of Embrapa Agrossilvipastoril in Sinop - Mato Grosso, it was cut and ensiled in experimental silos of PVC, maintaining the specific mass of 650 kg / m3. The material was submitted to the different...
Tipo: Separatas
Palavras-chave: Crude glycerine; Ensilage; Glycerine.; Silage; Enterobacteriaceae; Microorganisms.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086323

Cultivable autochthonous bacteria of the intestinal mucosa of Arapaima gigas (Pisces: Arapaimidae) with probiotic potential.
Provedor de dados: 119
Autores: PROIETTI-JÚNIOR, A. A.; LIMA, L. S.; GONÇALVES, V. D.; PRIBUL, B. R.; RODRIGUES, D. P.; TAVARES-DIAS, M.
Tipo: Artigo de periódico
Palavras-chave: Diversity; Microbiota; Peixe de Água Doce; Pirarucu; Enterobacteriaceae; Virulence; Freshwater fish.
Ano: 2021
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131390

Culture-independent approach for endosymbiotic bacterial systematics in Pentatomidae.
Provedor de dados: 119
Autores: PRADO, S. de S.; ZUCCHI, T.; PANIZZI, A. R.; CÔNSOLI, F. L.
Tipo: Separatas
Palavras-chave: Endosymbiotic bacterial systematics; Pentatomidae; Enterobacteriaceae.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/917184

Microbiological quality of pig carcasses in a slaughterhouse under risk-based inspection system.
Provedor de dados: 119
Autores: CAVALHEIRO, L. G.; GENÉ, L. A.; COLDEBELLA, A.; KICH, J. D.; RUIZ, V. L. de A.
Tipo: Artigo de periódico
Palavras-chave: Risk-based inspection; Pork processing; Mesophilic aerobic counts; Inspeção baseada em risco; Processamento de carne suína; Contagens aeróbicas mesofílicas; Qualidade da carne; Produção de Carne; Segurança Alimentar; Porco Tipo Carne; Controle de Qualidade; Meat production; Food safety; Food Safety and Inspection Service; Salmonella enterica; Enterobacteriaceae; Meat quality.
Ano: 2022
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149770

Diversidad de ß-lactamasas en aislamientos clínicos de enterobacterias
Provedor de dados: 38
Autores: Casabonne,Cecilia; Pérez,Jorgelina; Balagué,Claudia; Fernández,Luisa.
La rápida emergencia de resistencia a antimicrobianos debida a la presencia de b-lactamasas de espectro extendido (BLEE) tiene un impacto significativo en la salud pública. Las BLEEs son enzimas producidas por bacilos gramnegativos y confieren resistencia a las penicilinas, a todas las cefalosporinas y al aztreonam, pero no a los carbapenemes ni a las cefamicinas y la mayoría son inhibidas por el ácido clavulánico. El objetivo de este trabajo fue evaluar la resistencia a antibióticos b-lactámicos en aislamientos de Klebsiella pneumoniae, Escherichia coli y Proteus mirabilis y caracterizar las b-lactamasas responsables de dicha resistencia. Se analizaron 2.030 aislamientos (362 Klebsiella pneumoniae, 1.250 Escherichia coli y 175 Proteus mirabilis)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SS-lactamasas de espectro extendido; Enterobacteriaceae; Reacción en cadena de la polimerasa.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000300009

Prevalência de enterobactérias em Bothrops jararaca no Estado de São Paulo: levantamento microbiológico e padrões de resistência antimicrobiana - DOI: 10.4025/actascibiolsci.v30i3.536
Provedor de dados: 3
Autores: Bastos, Henrique Marçal; Lopes, Luiz Fernando Larangeira; USP - São Paulo; Gattamorta, Marco Aurélio; UNICAMP - Campinas; Matushima, Eliana Reiko; USP - São Paulo.
Existem poucos levantamentos microbiológicos em répteis no Brasil. O estudo aqui descrito enfoca uma espécie de serpente de grande interesse médico e serve como base para outros estudos e comparações. As amostras foram colhidas diretamente do cólon de jararacas adultas e saudáveis. O material foi semeado em ágar MacConkey (Difco®) e ágar XLT4 (Difco®). Os isolados foram identificados por meio do Sistema API 20E de Identificação Bioquímica (Galerias  API-BioMériux®). Os isolados de Salmonella sp. foram submetidos à sorotipagem. Por fim, as colônias foram submetidas ao teste de sensibilidade antimicrobiana. Foram obtidos vários gêneros da família Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus,...
Palavras-chave: 2.00.00.00-6    Ciências Biológicas Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella. Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella.
Ano: 2008
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/536

Produção de ácido indol acético por microorganismos solubilizadores de fosfato e sua interação com fungos micorrízicos arbusculares - DOI: 10.4025/actascibiolsci.v29i3.492
Provedor de dados: 3
Autores: Souchie, Edson Luis; cefet- Goiás; Azcón, Rosario; CSIC; Barea, Jose Miguel; CSIC; Saggin-Júnior, Orivaldo José; EMBRAPA-RJ; Silva, Eliane Maria Ribeiro da; Embrapa.
Avaliou-se o potencial de produção de ácido indol acético (AIA) in vitro por cinco isolados de fungo solubilizador de fosfato (FSF) do gênero Aspergillus sp. e quatro isolados de bactéria solubilizadora de fosfato (BSF) da família Enterobacteriaceae. Os isolados de bactéria e fungo foram incubados em meio líquido a 28ºC, por dois e quatro dias, respectivamente. Além disso, dois isolados de BSF da família Enterobacteriaceae (BSF 8 e BSF 56) foram avaliados quanto à capacidade de estimular ou inibir a germinação de esporos e o desenvolvimento micelial de duas espécies de fungos micorrízicos arbusculares (FMAs) in vitro. Foram instalados dois ensaios em placas de Petri, contendo agar-água (0,8%); um, utilizando o FMA Gigaspora margarita e o outro, com o FMA...
Palavras-chave: 2.12.00.00-9    Microbiologia sinergismo; Enterobacteriaceae; Regulador vegetal; Aspergillus sp.; Gigaspora margarita; Scutellospora sp.
Ano: 2007
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/492

Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ - Brazil
Provedor de dados: 42
Autores: Souchie,Edson L.; Saggin-Júnior,Orivaldo J.; Silva,Eliane M.R.; Campello,Eduardo F.C.; Azcón,Rosario; Barea,Jose M.
Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi, were evaluated in two different ecosystems. Samplings taken from two areas of Atlantic forest, in Paraty - RJ, Brazil, one with a secondary forest and the other with a grass pasture were studied. Four growth media: GL (glucose and yeast extract), GES (glucose, soil extract, KNO3, CaCl2, MgSO4, NaCl, FeEDTA and micronutrients solution), GAGES (glucose, soil extract, arabinose, glycerol, CaCl2, MgSO4 and NaCl) and GELP (glucose, soil extract, yeast extract, peptone, CaCl2, MgSO4 and NaCl) were evaluated for the isolation of P-solubilizing microorganisms. The identification of P-solubilizing bacteria was based on 16 S rDNA sequence analysis, while the identification of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphate; Enterobacteriaceae; Atlantic Forest; Glomeromycota; Soil microorganisms.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100016

Detecção e avaliação do perfil de sensibilidade antimicrobiana de enterobactérias isoladas de periquitos cara-suja (Pyrrhura griseipectus) em cativeiro
Provedor de dados: 45
Autores: Machado,D.N.; Lopes,E.S.; Albuquerque,Á.H.; Bezerra,W.G.A.; Horn,R.V.; Lima,S.V.G.; Siqueira,R.A.S.; Beleza,A.J.F.; Oliveira,F.R.; Cardoso,W.M.; Teixeira,R.S.C.
ABSTRACT This study was conducted to detect enterobacteria in captive Grey-breasted parakeet from a commercial breeding located in the state of Ceara. 25 individual cloacal swabs were collected and this material was subjected to microbiological processing to obtain the bacterial isolation, identification, and evaluation of the sensitivity profile to antimicrobials. The bacterial isolation in ten samples was determined, belonging to the genera Enterobacter, Hafnia, Serratia, Escherichia, Pantoea and Klebsiella, in which these last three corresponded to the bacteria most isolated, with respective percentages of 28.6%, 21.4%, and 21.4%. As for the sensitivity profile, antibiotics to which the bacteria showed more resistance were trimethoprim-sulfamethoxazole...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolamento; Enterobacteriaceae; Psitacídeos; Resistência.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601732

Investigação de Salmonella spp. em Psittaciformes exóticos e nativos mantidos em cativeiro na região central do Rio Grande do Sul
Provedor de dados: 45
Autores: Murer,L.; Didoné,S.R.; Freitas,A.B.B.; Lovato,M.
RESUMO As doenças infecciosas são reconhecidamente causadoras de declínios populacionais de animais silvestres e algumas delas podem representar ameaça à saúde pública. O presente estudo objetivou investigar a ocorrência de Salmonella spp. em Psittaciformes exóticos e nativos mantidos em cativeiro na região central do Rio Grande do Sul, além de comparar os resultados obtidos por meio do método bacteriológico convencional e da reação em cadeia da polimerase (PCR), mediante a utilização de material fecal do ambiente, evitando-se o estresse da contenção. Durante os meses de agosto/2016 e setembro/2016 foram coletadas amostras de fezes frescas de 90 gaiolas em dois criatórios, representando 180 aves. Com as duas técnicas empregadas nas análises, as amostras...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aves silvestres; Psitacídeos; Patógenos intestinais; Enterobacteriaceae; Zoonoses.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300815

Influence of the cleaning system of conveyor belts on microbiological quality of poultry meat
Provedor de dados: 45
Autores: Julião,T.A.; Cortezi,A.M.; Rossi,G.A.M.; D’Abreu,L.F.; Balieiro,J.C.C.; Vidal,A.M.C.
ABSTRACT This study focused on assessing the microbiology of conveyor belts surfaces, cleaned or not with pressurized water, and verification of the influence of this process on the microbiological quality of poultry meat. A reduction on mesophilic, psychrotrophic and Enterobacteriaceae counts (P&lt;0.05) on dry conveyor belts compared to wet ones was observed. For the chicken leg (consisting of drumstick and thigh) samples, no statistically significant differences were detected on counts of indicators of microorganisms on poultry legs (composed by drumstick and thigh). For poultry meat conducted on wet or dry conveyor belts, 99% and 86%, were positive for Listeria spp, respectively. Only one sample of chicken leg was positive for presence of L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Listeria monocytogenes; Food microbiology; Staphylococcus spp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401325

Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
Provedor de dados: 48
Autores: CHARRIER,MAR YVONNE; FONTY,GERARD; GAILLARD-MARTINIE,BRIGITTE; AINOUCHE,KADER; ANDANT,GERARD.
The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared &amp;#8805; 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium,...
Tipo: Journal article
Palavras-chave: Clostridium; Edible snails; Enterobacteriaceae; Enterococci; Gut microbiota; Lactococci.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010

Emergence of colistin resistance in the largest university hospital complex of São Paulo, Brazil, over five years
Provedor de dados: 55
Autores: Rossi,Flávia; Girardello,Raquel; Cury,Ana Paula; Di Gioia,Thais Sabato Romano; Almeida Jr,João Nóbrega de; Duarte,Alberto José da Silva.
Abstract Colistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clínicas, São Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gram-negative bacilli were evaluated. Enterobacteriaceae was the most frequent (86.1%) pathogen isolated, followed by Acinetobacter spp. (7.6%), and Pseudomonas spp. (6.3%). By temporal analysis there was a trend for an increase of colistin resistance among Enterobacteriaceae, but not among...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Colistin-resistant; Polymyxin B-resistant; Enterobacteriaceae; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100098

Ceftolozane-tazobactam activity against drug-resistant Enterobacteriaceae and Pseudomonas aeruginosa causing healthcare-associated infections in Latin America: report from an antimicrobial surveillance program (2013-2015)
Provedor de dados: 55
Autores: Pfaller,Michael A.; Shortridge,Dee; Sader,Helio S.; Gales,Ana; Castanheira,Mariana; Flamm,Robert K.
ABSTRACT This study evaluated the in vitro activity of ceftolozane-tazobactam and comparator agents tested against Latin American isolates of Enterobacteriaceae and Pseudomonas aeruginosa from patients with health care-associated infections. Ceftolozane-tazobactam is an antipseudomonal cephalosporin combined with a well-established β-lactamase inhibitor.A total of 2415 Gram-negative organisms (537 P. aeruginosa and 1878 Enterobacteriaceae) were consecutively collected in 12 medical centers located in four Latin American countries. The organisms were tested for susceptibility by broth microdilution methods as described by the CLSI M07-A10 document and the results interpreted according to EUCAST and CLSI breakpoint criteria.  Results: Ceftolozane-tazobactam...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceftolozane-tazobactam; Drug resistance; Enterobacteriaceae; P. aeruginosa; Latin America; Surveillance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600627

Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil
Provedor de dados: 55
Autores: Nogueira,Keite da Silva; Higuti,Ilma Hiroko; Nascimento,Agnaldo José do; Terasawa,Larissa Bail; Oliveira,Simone de; Matos,Adriana Pereira; Souza,Helena Aguilar Peres Homem de Mello de; Cogo,Laura Lúcia; Dalla Costa,Libera Maria.
Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL; Beta-lactamases; Enterobacteriaceae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006

Plasmid-mediated extended-spectrum beta-lactamase-producing strains of Enterobacteriaceae isolated from diabetes foot infections in a Brazilian diabetic center
Provedor de dados: 55
Autores: Motta,R.N.; Oliveira,M.M.; Magalhães,P.S.F.; Dias,A.M.; Aragão,L.P.; Forti,A.C.; Carvalho,C.B.M.
We bacteriologically analyzed 156 species of Enterobacteriaceae, isolated from 138 patients with community-acquired diabetic foot ulcers, in a prospective study made at a diabetic center and at the Federal University of Ceará, Brazil, from March, 2000, to November, 2001.The samples were cultured using selective media, and identification, susceptibility tests and detection of plasmid-mediated-extended-spectrum-beta-lactamase (ESBL) producing strains were made with conventional and automated methods. The most frequently occurring pathogens were K. pneumoniae (21.2%), Morganella morganii (19.9%) and E. coli (15.4%). High resistance rates were noted for ampicillin, first generation cephalosporin, trimethoprim/sulfamethoxazole, tetracycline,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL-producing strains; Antimicrobial resistance; Enterobacteriaceae.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200006

Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units
Provedor de dados: 55
Autores: Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes.
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Carbapenems; Enterobacteriaceae; KPC; Surveillance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436

Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates
Provedor de dados: 55
Autores: Gharout-Sait,Alima; Touati,Abdelaziz; Guillard,Thomas; Brasme,Lucien; Champs,Christophe de.
In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates.Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4- producing isolates and one DHA-1-producing Klebsiella pneumoniae . All AmpC-producing isolates co-expressed the broad-spectrum TEM-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; AmpC β-lactamases; Genotyping; Algeria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200187

Emergence of Klebsiella pneumoniae-producing KPC-2 carbapenemase in Paraíba, Northeastern Brazil
Provedor de dados: 55
Autores: Fehlberg,Lorena C.C.; Carvalho,Albalucia M.C.; Campana,Eloiza H.; Gontijo-Filho,Paulo P.; Gales,Ana C.
The emergence of KPC-2 producing K. pneumoniae in hospitalized patients at the intensive care unit (ICU) of a teaching hospital located in the city of João Pessoa, Paraíba, Brazil, is reported. Seven carbapenem-resistant K. pneumoniae recovered from different body sites of infection were analyzed. Most isolates showed a multidrug-resistance phenotype. Genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype A, which belongs to ST 437. These isolates also carry the encoding genes of five other betalactamases.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Beta-lactam resistance; Modified Hodge test; Carbapenem resistance; Enterobacteriaceae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600013

Molecular epidemiology of CTX-M producing Enterobacteriaceae isolated from bloodstream infections in Rio de Janeiro, Brazil: emergence of CTX-M-15
Provedor de dados: 55
Autores: Seki,Liliane Miyuki; Pereira,Polyana Silva; Conceição,Magda de Souza; Souza,Maria José; Marques,Elizabeth Andrade; Carballido,Jupira Miron; Carvalho,Maria Elisabeth Serqueira de; Assef,Ana Paula D'Alincourt Carvalho; Asensi,Marise Dutra.
OBJECTIVE: The present studywas designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil. MATERIAL AND METHODS: A total of 231 nonduplicate Enterobacteriaceae were isolated from five Brazilian hospitals between September 2007 and September 2008. The antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical Laboratory Standard Institute. Isolates showing resistance to third-generation cephalosporins were screened for ESBL activity by the double-disk synergy test. The presence of blaCTX-M , blaCTX-M-15 and blaKPC genes was determined by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloodstream infections; Enterobacteriaceae; CTX-M-15; PFGE; KPC; Brazil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600003

Cefepime versus extended spectrum β-lactamase-producing Enterobacteriaceae
Provedor de dados: 55
Autores: Nogueira,Keite da Silva; Daur,Alessandra Vale; Reason,Iara Taborda de Messias; Gales,Ana Cristina; Costa,Libera Maria Dalla.
The objective of this study was to evaluate the susceptibility to cefepime of a large group of ESBL- producing enterobacteria recently isolated in a Brazilian teaching hospital . The study included 280 strains of ESBL-producing enterobacteria, isolated between 2005 and 2008. The presence of the genes blaCTX-M, blaTEM and blaSHV was determined by PCR and confirmed by nucleotide sequencing. Susceptibility testing for cefepime was performed by disc-diffusion, agar dilution method and E-test®. Among the isolates, 34 (12.1%) presented a cefepime inhibition zone &amp;gt; 21 and MIC &lt; 8 mg/L by agar dilution and E-strip methods. The use of cefepime for the treatment of infections caused by ESBL-producing bacteria has been controversial. Some studies of PD/PK...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Beta-lactamases; Cephalosporin resistance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200014

Enterobacteriaceae resistant to third generation cephalosporins upon hospital admission: risk factors and clinical outcomes
Provedor de dados: 55
Autores: Oliveira,Mirian Cristina; Oliveira,Clara Rodrigues Alves; Gonçalves,Karine Valéria; Santos,Marciléa Silva; Tardelli,Amanda Cristina Silva; Nobre Jr.,Vandack Alencar.
Objectives: Evaluate risk factors and clinical outcomes of infections caused by Enterobacteriaceae resistant to third-generation cephalosporins present in samples collected upon hospital admission.Methods: Risk factors were evaluated using a 1:2 ratio case-control study. Influence of resistance on the appropriateness of antibiotic therapy, length of stay, and hospital mortality were prospectively evaluated. Characteristics independently associated with the presence of resistant enterobacteria were assessed by logistic regression.Results: Enterobacteria resistant to third-generation cephalosporins were quite common (26.0%). Male gender (OR: 2.66; 95% CI, 1.17-5.06; p= 0.019), invasive prosthesis (OR: 3.79; 95% CI, 1.29-11.08; p= 0.015), previous use of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Third generation cephalosporins; Risk factors; Antibiotic resistance.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300239

Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal
Provedor de dados: 55
Autores: Baral,Pankaj; Neupanea,Sanjiv; Shresthac,Basudha; Ghimirea,Kashi Ram; Marasinia,Bishnu Prasad; Lekhaka,Binod.
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838-9.817; p &lt;...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: AmpC β-lactamase; Enterobacteriaceae; Antibiotic resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200022

Efﬁcacy of polymyxins in the treatment of carbapenem-resistant Enterobacteriaceae infections: a systematic review and meta-analysis
Provedor de dados: 55
Autores: Ni,Wentao; Cai,Xuejiu; Wei,Chuanqi; Di,Xiuzhen; Cui,Junchang; Wang,Rui; Liu,Youning.
In recent years, carbapenem-resistant Enterobacteriaceae has become endemic in many countries. Because of limited treatment options, the abandoned "old antibiotics", polymyxins, have been reintroduced to the clinic. To evaluate the clinical efﬁcacy of polymyxins in the treatment of infections caused by carbapenem-resistant Enterobacteriaceae , we systemically searched the PubMed, Embase, and Cochrane Library databases and analyzed the available evidence. The Preferred Reporting Items for Systematic reviews and Meta-Analysis statement were followed, and the I 2 method was used for heterogeneity. Nineteen controlled and six single-arm cohort studies comprising 1086 patients met the inclusion criteria. For controlled studies, no signiﬁcant difference was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymyxin; Enterobacteriaceae; Carbapenem-resistant; Carbapenemase-producing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200170

Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates
Provedor de dados: 56
Autores: Quiles,M.G.; Rocchetti,T.T.; Fehlberg,L.C.; Kusano,E.J.U.; Chebabo,A.; Pereira,R.M.G.; Gales,A.C.; Pignatari,A.C.C.
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and was associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), a 16S rRNA methylase not previously reported in Brazil, in two distinct strains of Enterobacter cloacae. Our results suggested that the introduction of blaNDM-1 in Brazil has been accompanied by rapid spread, since our isolates showed no genetic relationship.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Enterobacteriaceae; Carbapenem-resistance; Carbapenemases; Methylase.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200174

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins
Provedor de dados: 58
Autores: Sampaio,Jorge Luiz Mello; Gales,Ana Cristina.
ABSTRACT During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL; KPC; NDM; Extended spectrum β-lactamases; New Delhi metallo-β-lactamase; Klebsiella pneumoniae carbapenemase; Polymyxin B; Colistin; Antimicrobial resistance; Carbapenemases; Brazil; Enterobacteriaceae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500031

Occurrence and characteristics of extended spectrum beta-lactamases-producing Enterobacteriaceae from foods of animal origin
Provedor de dados: 58
Autores: Tekiner,İsmail Hakkı; Özpınar,Haydar.
Abstract Presence of extended spectrum beta-lactamases (ESBL) in bacteria is a growing health concern of global significance. The local, regional, national, and international epidemiological studies for extended spectrum beta-lactamases-producing Enterobacteriaceae and their encoding genes in foods are still incomplete. The objective of this study was to determine the occurrence of extended spectrum beta-lactamases-producing Enterobacteriaceae and the characteristics of their encoding genes from a total of 250 samples of various foods of animal-origin (100 raw chicken meat, 100 raw cow milk, and 50 raw cow milk cheese) sold in Turkey. Overall, 55 isolates were positive as extended spectrum beta-lactamases-producing Enterobacteriaceae. The most prevalent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bla gene; Enterobacteriaceae; ESBL; Public health; Turkey.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200444

Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
Provedor de dados: 58
Autores: Moreira,Maria Aparecida S.; Rodrigues,Patrícia P.C.F.; Tomaz,Rafael S.; Moraes,Célia A. de.
Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multiresistance; Antimicrobials; Enterobacteriaceae; Proton-motive force; Diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200007

Isolation of Salmonella spp. in cattle egrets (Bubulcus ibis) from Fernando de Noronha Archipelago, Brazil
Provedor de dados: 58
Autores: Silva,Marcio A.; Fernandes,Érika F.S.T.; Santana,Sandra C.; Marvulo,Maria Fernanda V.; Barros,Mércia R.; Vilela,Sineide M.O.; Reis,Eliane M.F.; Mota,Rinaldo A.; Silva,Jean C.R.
Abstract The growth of the population of cattle egrets (Bubulcus ibis) in the archipelago of Fernando de Noronha constitutes a threat to public health and biological diversity because of their competition with and predation on native species and the possibility of transmission of pathogens to human beings, livestock and native wildlife. The aim here was to search for, isolate and identify serovars of Salmonella in clinically healthy local cattle egrets. Cloacal swabs were obtained from 456 clinically healthy cattle egrets of both sexes and a variety of ages. The swabs were divided into 51 pools. Six of these (11.7%) presented four serovars of Salmonella enterica subspecies enterica: Salmonella serovar Typhimurium; Salmonella serovar Newport; Salmonella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ardeidae; Oceanic islands; Conservation medicine; Enterobacteriaceae; Public health.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300559

Enterobacteriaceae in mouth and cloaca of podocnemis expansa and P. Unifilis (testudines: chelonia) populations of national park of araguaia plains, Brazil
Provedor de dados: 58
Autores: Morais,Paula Benevides de; Souza,Denise Rodrigues de; Sousa,Francisca Maria Pinheiro de; Oliveira,Kleverson Wessel de; Pimenta,Raphael Sanzio.
Shigella flexnerii and Escherichia coli were the most frequent Gram-negative bacteria found in the mouth cavity and cloacae of the turtles Podocnemis expansa and P. unifilis on beaches in the National Park of Araguaia, Brazil. Reptiles are known as Salmonella carriers, despite rarely isolated in these turtles.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Shigella; Enterobacteriaceae; Turtles; Bananal Island.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200017

Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk
Provedor de dados: 58
Autores: Cirolini,Andréia; Miotto,Marília; Machado,Fernanda Morgana; Silva,Helen Silvestre da; Ogliari,Paulo José; Vieira,Cleide Rosana Werneck.
The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Milk; Alternative methods; ISO 21528-2:2004; Count.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300016

Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL)
Provedor de dados: 58
Autores: Silva,Carlos Henrique Pessôa de Menezes e.
The new ß-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum ß-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Culture medium; ESBL.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400006

Occurrence of yeasts, pseudomonads and enteric bacteria in the oral cavity of patients undergoing head and neck radiotherapy
Provedor de dados: 58
Autores: Gaetti-Jardim Júnior,Elerson; Ciesielski,Francisco Isaak Nicolas; Sousa,Fátima Regina Nunes de; Nwaokorie,Francisca; Schweitzer,Christiane Marie; Avila-Campos,Mario Júlio.
The aim of this study was to evaluate the occurrence of yeasts, pseudomonads and enteric bacteria in the oral cavity of patients undergoing radiotherapy (RT) for treatment of head and neck cancer. Fifty patients receiving RT were examined before, during and 30 days after RT. Saliva, mucosa, and biofilm samples were collected and microorganisms were detected by culture and polymerase chain reaction (PCR). The most prevalent yeasts in patients submitted to RT were Candida albicans, C. tropicalis, C. krusei, C. glabrata and C. parapsilosis. Citrobacter, Enterobacter, Enterococcus, Klebsiella, Proteus, and Pseudomonas were the most frequently cultivated bacteria. Before RT, targeted bacteria were cultivated from 22.2% of edentulous patients and 16.6% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiotherapy; Cancer; Enterobacteriaceae; Prevalence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300024

Opportunistic microorganisms in patients undergoing antibiotic therapy for pulmonary tuberculosis
Provedor de dados: 58
Autores: Querido,Silvia Maria Rodrigues; Back-Brito,Graziella Nuernberg; Santos,Silvana Soléo Ferreira dos; Leão,Mariella Vieira Pereira; Koga-Ito,Cristiane Yumi; Jorge,Antonio Olavo Cardoso.
Antimicrobial therapy may cause changes in the resident oral microbiota, with the increase of opportunistic pathogens. The aim of this study was to compare the prevalence of Candida, Staphylococcus, Pseudomonas and Enterobacteriaceae in the oral cavity of fifty patients undergoing antibiotic therapy for pulmonary tuberculosis and systemically healthy controls. Oral rinsing and subgingival samples were obtained, plated in Sabouraud dextrose agar with chloramphenicol, mannitol agar and MacConkey agar, and incubated for 48 h at 37ºC. Candida spp. and coagulase-positive staphylococci were identified by phenotypic tests, C. dubliniensis, by multiplex PCR, and coagulase-negative staphylococci, Enterobacteriaceae and Pseudomonas spp., by the API systems. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Candida; Enterobacteriaceae; Pseudomonas spp; Staphylococcus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400012

Extended-spectrum β-lactamase-producing enterobacteriaceae in community-acquired urinary tract infections in São Luís, Brazil
Provedor de dados: 58
Autores: Abreu,Afonso Gomes; Marques,Sirlei Garcia; Monteiro-Neto,Valério; Gonçalves,Azizedite Guedes.
The number of ESBL-producing Enterobacteriaceae in community-acquired urinary tract infections worldwide is probably underestimated because of the technical difficulties encountered with their detection. In this study, out of 5,672 urine samples analyzed, 916 were positive for uropathogens, 472 of them being enterobacteria of which 7.6% produced β-lactamases. Analysis of the isolated from 36 patients showed a high level of antibiotic resistance, with 52.7% and 80.5% of isolates expressing blaTEM and blaCTX-M, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Antimicrobial resistance; ESBL; Community-acquired infections.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200020

Mutations in the quinolone resistance-determining regions of gyrA and parC in Enterobacteriaceae isolates from Brazil
Provedor de dados: 58
Autores: Minarini,Luciene A. R.; Darini,Ana Lucia C.
Mutations in the quinolone resistance-determining regions (QRDR) in chromosomal gyrA and parC genes and fluoroquinolone susceptibility profiles were investigated in quinolone-resistant Enterobacteriaceae isolated from community and hospitalized patientsin the Brazilian Southeast region. A total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to 2005 were investigated for mutations in the topoisomerases genes gyrA and parC by amplifying and sequencing the QRDR regions. Susceptibility to fluoroquinolones was tested by the agar dilution method. Amongst the 112 enterobacterial isolates, 81 (72.3%) were resistant to ciprofloxacin and 5 (4.5%) showed reduced susceptibility. Twenty-six (23.2%) were susceptible to ciprofloxacin....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quinolone resistance-determining region; Mutations; GyrA; ParC; Enterobacteriaceae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400010

Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
Provedor de dados: 58
Autores: Oliveira,Caio Fernando de; Salla,Adenilde; Lara,Valéria Maria; Rieger,Alexandre; Horta,Jorge André; Alves,Sydney Hartz.
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ESBL; Enterobacteriaceae; Prevalence; PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200002

Frequency of zoonotic bacteria among illegally traded wild birds in Rio de Janeiro
Provedor de dados: 58
Autores: Matias,Carlos Alexandre Rey; Pereira,Ingrid Annes; Reis,Eliane Moura Falavina dos; Rodrigues,Dália dos Prazeres; Siciliano,Salvatore.
Abstract The illegal wildlife trade may increase the risk of infectious disease transmission, and it may not only cause disease outbreaks in humans but also threaten livestock, native wild populations, and ecosystems' health. Bird species may act as carriers in the transmission of enteric pathogens. However, epidemiological studies on zoonotic bacteria in wild birds are rare in Brazil. From March 2011 to March 2012, we investigated the frequency of Enterobacteriaceae in cloacal swab samples from 109 birds of the passerine and Psittacidae families. These birds were recovered from illegal trade in Rio de Janeiro, Brazil, and sent to a rehabilitation center. Gram-negative bacteria were isolated from 86 wild birds (78.9%). A mean (±SD) of 1.68 (±1.30)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antibiotic resistance; Enterobacteriaceae; Public health; Salmonella; Wild birds.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400882

High prevalence rate of extended-spectrum beta-lactamases (ESBL) among Enterobacteriaceae in a small Brazilian public hospital
Provedor de dados: 60
Autores: Lenhard-Vidal,Adriane; Cardoso,Rosilene Fressatti; Pádua,Rubia Andreia Falleiros de; Siqueira,Vera Lúcia Dias.
The production of extended-spectrum beta-lactamases (ESBL) is considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Data on culture and susceptibility tests were collected from the Clinical Analyses and Research Laboratory charts reporting on patients admitted to the University Hospital of Maringá (HUM) from January 2004 to December 2009. The following Enterobacteriaceae were selected: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter spp. and Proteus mirabilis. All tests were performed according to the recommendations of the Clinical and Laboratory Standards Institute (biochemical identification; susceptibility tests; initial screening and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extended-spectrum β-lactamases; Enterobacteriaceae; Bacterial resistance; Β-lactams.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400006

β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
Provedor de dados: 60
Autores: Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias.
ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609

Inspected and non-inspected chilled chicken carcasses commercialized in Viçosa, MG, Brazil: microbiological parameters and Salmonella spp. occurrence
Provedor de dados: 65
Autores: Cossi,Marcus Vinícius Coutinho; Almeida,Michelle Vieira de; Dias,Mariane Rezende; Pinto,Paulo Sérgio de Arruda; Nero,Luís Augusto.
Sixty samples of chilled chicken carcasses submitted (30) and not submitted (30) to Brazilian inspection services were analyzed to investigate if inspected and non-inspected chilled carcasses represented different food safety risks in the region of Viçosa, MG, Brazil. The mean counts of indicator microorganisms (mesophilic aerobes, Enterobacteriaceae, total coliforms and Escherichia coli) of samples belonging to the inspected and non-inspected lots did not present significant differences (P&amp;gt;0.05). Also, no significant differences (P&amp;gt;0.05) were observed for the numbers of Salmonella spp. and E. coli (higher than 2log cfu g-1) between samples submitted or not to inspection. Statistical differences were observed between the two sample classes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inspection; Salmonella; Escherichia coli; Enterobacteriaceae; Hygiene indicators.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900025

Effects of a dietary added formaldehyde-propionic acid blend on feed enterobacteria counts and on growing pig performance and fecal formaldehyde excretion
Provedor de dados: 65
Autores: Sbardella,Maicon; Perina,Danilo do Prado; Andrade,Carla de; Longo,Flavio Alves; Miyada,Valdomiro Shigueru.
The purpose of this study was to evaluate the effects of a dietary added formaldehyde-propionic acid blend on feed enterobacteria counts and on growing pig performance and fecal formaldehyde excretion. Forty pigs (34.2±1.8kg BW) were used in a randomized complete block design experiment with four treatments, five replications per treatment and two animals per experimental unit (pen). The treatments were: 0.0, 1.0, 2.0, and 3.0g of blend kg-1 of feed. No effects (P&gt;0.05) of dietary added blend levels were observed on average daily gain, average daily feed intake and feed:gain ratio during 1 to 14 and 1 to 28 days of experiment. Increasing dietary levels of blend reduced quadratically (P&lt;0.01) total Enterobacteriaceae counts on 1st and 14th day after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feed additive; Enterobacteriaceae; Swine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000300474

Isolamento de espécies enterobacterianas em Stomoxys calcitrans
Provedor de dados: 65
Autores: Castro,Bruno Gomes de; Souza,Miliane Moreira Soares de; Bittencourt,Avelino José.
Este estudo teve por objetivo relatar o isolamento de espécies enterobacterianas em segmentos de Stomoxys calcitrans coletadas, em propriedades rurais de dois municípios do Estado do Rio de Janeiro, Brasil. Nove das 28 espécies isoladas e identificadas ainda não haviam sido descritas na mosca dos estábulos. Algumas dessas espécies possuem destacado potencial patogênico ao homem e aos animais ou são oportunistas e outras não foram descritas como causadoras de enfermidades de importância médica ou veterinária.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mosca dos estábulos; Stomoxys calcitrans; Enterobacteriaceae; Gado leiteiro.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900043

Detection of resistance genes and evaluation of water quality at zoo lakes in Brazil
Provedor de dados: 65
Autores: Faria,Ana Carolina Silva de; Godoy,Isabela de; Sanches,Anderson Aparecido Amorim; Iglesias,Gabriela Accardi; Candido,Stefhano Luiz; Paz,Regina Célia Rodrigues da; Nakazato,Luciano; Dutra,Valéria.
ABSTRACT: The investigation of the presence of antibiotic-resistance genes in aquatic environments is important to identify possible reservoirs of resistant microorganisms that could be a threat to human and animal health. The aims of this study were to analyze the presence of genes conferring resistance to antimicrobials in the aquatic environment and to assess the quality of water in zoo lakes. Results showed a pattern of genes conferring resistance to multiple antibiotics and turbidity, which was expected to be due to the presence of contaminants. The most frequent genes were sul I and sul II (sulfonamides), which were present in all the lakes, followed by genes encoding β-lactamases such as blaPSE I (77.8%) and ampC (66.7%). However, tet(K), tet(M),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug resistance; Zoo pollution; Aquatic environment; Enterobacteriaceae.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500860

The dispersal of nitrogen-fixing Enterobacteriaceae from sewage into the waters and sediments of Morecambe Bay, UK.
Provedor de dados: 5
Autores: Jones, K; Betaieb, M.
The sediments of Morecambe Bay contain large numbers of salinity-tolerant, nitrogen-fixing Enterobacteriaceae (NFEs) mainly Klebsiella pneumoniae ) which appear to be derived from sewage. Sewage effluents, regardless as to whether they have received primary or secondary treatment, contain huge numbers of NFEs with salinity-tolerant and freshwater strains present in roughly equal quantities. The bacteria are discharged into the rivers and estuaries and carried out to sea. Once in the sea the salinity-tolerant strains retain their viability better than the freshwater strains so that the incoming tide, which doses the sediments, contains mainly salinity-tolerant NFEs. It is probable that similar processes occur all round the U.K. coast, as inshore coastal...
Tipo: Text
Palavras-chave: Klebsiella pneumoniae; Enterobacteriaceae; Salinity tolerance; Microbial contamination; Pathogenic bacteria; Bacteria; Sewage; Nitrogen fixation; Sediment pollution; Sea water.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-1003.pdf

Development of a real-time PCR method coupled with a selective pre-enrichment step for quantification of Morganella morganii and Morganella psychrotolerans in fish products
Provedor de dados: 5
Autores: Podeur, Gaetan; Dalgaard, Paw; Leroi, Francoise; Prevost, Herve; Ernborg, Jette; Martinussen, Jan; Hansen, Lars Hestbjerg; Filet, Marie-france.
Histamine fish poisoning is common and due to toxic concentrations of histamine often produced by Gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. The genus Morganella includes two species previously reported to cause incidents of histamine fish poisoning. Morganella morganii and Morganella psychrotolerans are both strong producer of histamine. However, little is known about the occurrence and critical stages for fish contamination with these bacteria. To elucidate contamination routes of Morganella, specific real-time quantitative PCR (RTi qPCR) methods for quantification of M. morganii and M. psychrotolerans have been developed. Selective primers amplified a 110 bp region of the vasD gene for M....
Tipo: Text
Palavras-chave: Histamine-producing bacteria; Tuna; Selective medium; Enterobacteriaceae; Galactokinase; Type VI secretion system.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00254/36551/35096.pdf

Environmental parameters and antimicrobial susceptibility of enterobacteriaceae isolated from estuarine waters of São Vicente, São Paulo state, Brazil
Provedor de dados: 80
Autores: Lourenço,N. G. G. S.; Takahashi,C. K.; Lopes,T. F.; Lopes,C. A. M.
Diseases transmitted by water consists a serious public health problem and enterobacteria are the main group of microorganisms responsible for outbreaks in humans. Such pathogenic bacteria proliferate in water polluted by domestic and industrial sewage and reach the population through seawater contact. The aim of the present work was to study environmental parameters as well as to identify Enterobacteriaceae species and their antimicrobial susceptibility in water samples collected from the estuarine area of São Vicente city (São Paulo State, Brazil). Strains were identified by using traditional biochemical tests described in literature and antimicrobial susceptibility tests were carried out using the disk diffusion method. Out of 26 samples, Escherichia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Estuarine water; Environmental parameters; Enterobacteriaceae; Antimicrobial susceptibility.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200006

Inhibición de los contaminantes del tempeh de soya
Provedor de dados: 20
Autores: Mejías, E.; Delgado, R.; Silveira, I.; Lafargue, D.; Cárdenas, E.
Se evaluó el efecto de diferentes especias o condimentos sobre la calidad microbiológica del tempeh y la posible acción inhibidora de sus contaminantes. El tempeh se obtuvo a partir de frijol de soya el cual se sometió a los procesos de cocción, escurrimiento y fermentación. A continuación se procedió a incorporar los condimentos ensayados en dos proporciones por separado a la soya fresca antes de su inoculación con la cepa R. oligosporus, se mezclaron bien y se sometieron al proceso de fermentación, el cual tuvo lugar en bandejas de poliestireno, incubadas a 30 ± 2 ºC por un período de 18 a 20 h hasta obtener un producto con las características típicas del tempeh. De éste se tomaron muestras para determinar contenido de humedad, conteo total de viables y...
Tipo: Journal Contribution
Palavras-chave: Biological contamination; Chemical activity; Tempeh; Bacterial contamination; Enterobacteriaceae; Inhibition; Garlic; Anions; Spices.
Ano: 2008
URL: http://hdl.handle.net/1834/5010

บทบาทของเชื้อแบคทีเรียในทางเดินอาหารของแมลงวันผลไม้ (Diptera: Tephritidae)
Provedor de dados: 106
Autores: Narit Thaochan.
Tipo: PhysicalObject
Palavras-chave: Fruit flies; Diptera; Tephritidae; Bactrocera dorsalis; Enterobacteriaceae; Symbiosis; Parasitism; Gut bacteria; Biodiversity; Behaviour; Growth; แมลงวันผลไม้; เชื้อแบคทีเรีย; ภาวะแบบพึ่งพาอาศัยกัน; ภาวะที่เป็นโทษต่อกัน; ทางเดินอาหาร; ความหลากหลายทางชนิด; กระบวนการเจริญเติบโต; การแสดงพฤติกรรม; การควบคุมประชากร; การควบคุมแมลงวันผลไม้.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5765