Consulta: subjectFacets:"Enterotoxigenic Escherichia coli"
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Data/hora: 17/06/2024 14:18:51

Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum
Provedor de dados: 45
Autores: Figueiredo,H.C.P.; Lage,A.P.; Pereira Júnior,F.N.; Leite,R.C.
A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the blocks, each block consisting of two pregnant females (a vaccinated one and a control one) and their respective calves. In experiment A (farm 1), comprised of 18 blocks, the animals received a vaccine dose 30 days before delivery. In experiment B (farm 1), consisted of 26 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In experiment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calf; Enterotoxigenic Escherichia coli; Vaccine; Diarrhea; K99; F41.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400001

Enterotoxins, colonization factors, serotypes and antimicrobial resistance of enterotoxigenic Escherichia coli (ETEC) strains isolated from hospitalized children with diarrhea in Bolivia
Provedor de dados: 55
Autores: Rodas,Claudia; Mamani,Rosalía; Blanco,Jorge; Blanco,Jesus Eulogio; Wiklund,Gudrun; Svennerholm,Ann-Mari; Sjöling,Åsa; Iniguez,Volga.
Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of bacterial diarrhoea among children in Asia, Africa and Latin America but less investigated in Bolivia. OBJECTIVE: To determine the relation between enterotoxins, CFs and serotypes as well as the antimicrobial resistance patterns in a set of ETEC isolates collected from hospitalized children with acute diarrhea. In the present study we characterized 43 ETEC strains isolated from 2002 to 2006 from hospitalized children (0-5 years) with acute diarrhea in Bolivia. The strains were analyzed for heat-labile (LT) and heat-stable (ST) enterotoxins and colonization factor (CF) profiles, as well as for serogroups and antimicrobial resistance using phenotypic (ELISA, dot blot, slide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterotoxigenic Escherichia coli; Enterotoxins; Drug resistance; Bolivia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200007

A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli
Provedor de dados: 55
Autores: Bagheri,Samane; Gargari,Seyed Latif Mousavi; Rasooli,Iraj; Nazarian,Shahram; Alerasol,Masoome.
OBJECTIVES: Enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in children under 5, is an important agent for traveler's diarrhea. Heat-labile enterotoxin (LT) and colonization factors (CFs) are two main virulence mechanisms in ETEC. CS6 is one of the most prevalent CFs consisting of two structural subunits viz., CssA, CssB, necessary for attachment to the intestinal cells.METHODS: In the present research, a chimeric trivalent protein composed of CssB, CssA and LTB was constructed. The chimeric gene was synthesized with codon bias of E. coli for enhanced expression of the protein. Recombinant proteins were expressed and purified. Mice were immunized with the recombinant protein. The antibody titer and specificity of the immune sera were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterotoxigenic Escherichia coli; CS6; Enterotoxin; Chimeric protein.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300308

Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain
Provedor de dados: 56
Autores: Guillobel,H.C.R.; Luna,M.G.; Camacho,E.F.; Almeida,D.F.; Ferreira,L.C.S.
An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella typhimurium aroA; Enterotoxigenic Escherichia coli; Attenuated vaccines; Colonization factor antigen I.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012