Consulta: subjectFacets:"Enzyme-linked immunosorbent assay"
Registros recuperados: 10
Data/hora: 11/06/2024 00:33:42

USE OF AN ELISA ASSAY TO EVALUATE VENOM, ANTIVENOM, IgG AND IgM HUMAN ANTIBODY LEVELS IN SERUM AND CEREBROSPINAL FLUID FROM PATIENTS BITTEN BY Crotalus durissus terrificus IN BRAZIL
Provedor de dados: 79
Autores: BARRAVIERA,B.; SARTORI,A.; PEREIRA DA SILVA,M. F.; KANENO,R.; PERAÇOLI,M. T. S.
A sandwich-type ELISA technique for specific and sensitive detection of Crotalus durissus terrificus venom antigens, horse-antivenom, human IgG and IgM antibodies was set up. Sixteen patients, 13 males and 3 females aged between 13 to 63 years (mean 33 ± 15) bitten by Crotalus durissus terrificus snakes were studied. Of the 15 patients, 6 had previously received anti-Crotalus venom and no seric venom was detected. For the other 9 patients studied, the venom levels ranged from 2 to 108 ng/ml according to the severity of each case. Seric antivenom was detected up to 44 days after the bite. IgM human antibody levels against Crotalus venom were higher between 3 and 18 days after specific treatment. IgG human antibody levels against Crotalus venom were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzyme-linked immunosorbent assay; Crotalus durissus terrificus; Venom; Antivenom; Antibodies; Cerebrospinal fluid.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100003

Detection and neutralization of venom by ovine antiserum in experimental envenoming by Bothrops jararaca
Provedor de dados: 80
Autores: Peres,C. M.; Bastos,M. F.; Ferreira,J.; Sartori,A.
In this study we optimized an enzyme-linked immunosorbent assay (ELISA) to evaluate bothropic venom levels in biological samples. These samples were obtained by two distinct protocols. In the first one, Swiss mice were injected with 1 LD50 of Bothrops jararaca (B. jararaca) venom and 15 minutes later, animals were treated with ovine antibothropic serum. Blood and spleen homogenate samples were obtained 6 hours after antiserum therapy. Ovine antibothropic serum significantly neutralized venom levels in serum and spleen. In the second protocol, BALB/c mice were injected with 1 LD50 of bothropic venom by either intraperitoneal (IP) or intradermal (ID) route and venom levels were evaluated 1, 3 and 6 hours after, in blood, spleen homogenates and urine. Serum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Enzyme-linked immunosorbent assay; Ovine anti-bothropic serum; Venom neutralization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100010

Detection of shellfish toxins from scallops in Guangzhou seafood market
Provedor de dados: 80
Autores: Huazhang,L; Wen-xue,L; Quan-xin,Z; Guang-yu,Y; Jun-tao,L; Li,M; Wei,Z.
To evaluate scallop safety in the Guangzhou seafood market, contents of shellfish toxins in adductor muscle, mantle skirts, gills and visceral mass of scallops were examined using enzyme-linked immunosorbent assay (ELISA) and mouse unit assay. The results showed that: paralytic shellfish poisoning contents were up to 37.44 μg/100 g by ELISA and 319.99 MU/100 g by mouse unit assay, which did not exceed the limits of national standards (80 μg/100g and 400 MU/100 g); the contents of diarrhetic shellfish poisoning were 142.04 μg/100g and 0.2 MU/100 g, which exceeded the national standard limits (60 μg/100g); neurotoxic shellfish poisoning was undetectable; the contents of amnesic shellfish poisoning reached 220.12 μg/100g (no limit value could be referred to)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paralytic shellfish poisoning; Diarrheic shellfish poisoning; Neurotoxic shellfish poisoning; Amnesic shellfish poisoning; Enzyme-linked immunosorbent assay; Mouse unit assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100010

Seroepidemiological analysis of toxoplasmosis in college students
Provedor de dados: 80
Autores: Rodrigues,Jaqueline Polizeli; Frei,Fernando; Navarro,Italmar Teodorico; Silva,Luciana Pereira; Marcelino,Monica Yonashiro; Andrade-Junior,Heitor Franco de; Faria,Carolina Arruda de; Santos,Marislene; Ribeiro-Paes,João Tadeu.
Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Toxoplasmosis; Enzyme-linked immunosorbent assay; Indirect fluorescent antibody test; Seroprevalence; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100311

Development of a sensitive enzyme immunoassay (ELISA) for specific identification of Lachesis acrochorda venom
Provedor de dados: 80
Autores: Núñez Rangel,V; Fernández Culma,M; Rey-Suárez,P; Pereañez,JA.
The snake genus Lachesis provokes 2 to 3% of snakebites in Colombia every year. Two Lachesis species, L. acrochorda and L. muta, share habitats with snakes from another genus, namely Bothrops asper and B. atrox. Lachesis venom causes systemic and local effects such as swelling, hemorrhaging, myonecrosis, hemostatic disorders and nephrotoxic symptoms similar to those induced by Bothrops, Portidium and Bothriechis bites. Bothrops antivenoms neutralize a variety of Lachesis venom toxins. However, these products are unable to avoid coagulation problems provoked by Lachesis snakebites. Thus, it is important to ascertain whether the envenomation was caused by a Bothrops or Lachesis snake. The present study found enzyme linked immunosorbent assay (ELISA)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Lachesis; Lachesis acrochorda; Enzyme-linked immunosorbent assay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200007

Study of anticardiolipin antibody in hepatitis C virus-positive patients
Provedor de dados: 80
Autores: Elsayeh,H; Abdallah,N; Hamed,NA; Morsi,MG; Eldighidy,A; Kamal,HA.
Several antibodies, including anticardiolipin antibodies (ACA), have been detected among chronically infected hepatitis C virus (HCV) patients. The present work aimed at ascertaining the clinical significance of ACA levels among HCV infection associated with two commonly encountered diseases, thrombocytopenia and arteriovenous-shunt malfunction. Six groups were studied, 11 HCV-positive thrombocytopenic patients (group I), 14 HCV-positive non-thrombocytopenic patients (group II) and 15 healthy controls (group III), 11 anti-HCV-positive hemodialysis patients with non-functioning shunt (group IV), 14 anti-HCV-positive hemodialysis patients with patent shunt (group V) (Bain Medical Equipment Co., China) and 15 healthy controls (group VI). Anticardiolipin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C virus; Hemodialysis; Anticardiolipin antibody; Enzyme-linked immunosorbent assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400014

Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
Provedor de dados: 19
Autores: Terkawi, M. A.; Aboge, G.; Jia, H.; Goo, Y-K.; Ooka, H.; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawazu, Shin-ichiro; Fujisaki, K.; Xuan, Xuenan; 山岸, 潤也; 西川, 義文; 河津, 信一郎; 玄, 学南.
A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both...
Palavras-chave: Babesia gibsoni; Enzyme-linked immunosorbent assay; Deiagnostic performance.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2232

Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen
Provedor de dados: 19
Autores: Zhou, Jinlin; Jia, Honglin; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or...
Palavras-chave: Babesia gibsoni; Rhoptry-associated protein; Enzyme-linked immunosorbent assay; Diagnosis.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/811

Epidemiological survey of Babesia gibsoni infection in dogs in eastern Japan
Provedor de dados: 19
Autores: Miyama, Takako; Sakata, Yoshimi; Shimada, Yojiro; Ogino, Shoji; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; Nagasawa, Hideyuki; Inokuma, Hisashi.
To determine the distribution of Babesia gibsoni infection in dogs in the eastern part of Japan, an epidemiological survey of dogs suspected of having B. gibsoni infection was attempted using the polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Thirty-five of 115 such dogs (30.4%) were positive by PCR and/or ELISA. The 35 positive dogs consisted of 28 Tosa dogs, 4 American Pit Bull Terriers, and 3 mongrel dogs in Aomori, Fukushima, Ibaraki, Gunma, Chiba, Tokyo, Kanagawa, and Nagano Prefectures. The positive dogs had a significantly lower rate of tick exposure and a higher rate of bites by other dogs. Twenty-two of 35 B. gibsoni-positive dogs were infected with hemoplasma, and the rate of infection was significantly higher than...
Palavras-chave: Babesia gibsoni; Eastern Japan; Enzyme-linked immunosorbent assay; Hemoplasma; Polymerase chain reaction.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/924

Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
Provedor de dados: 58
Autores: Matos,Denise Cristina Souza; Marcovistz,Rugimar; Silva,Andréa Marques Vieira da; Quintilio,Wagner; Georgini,Ricardo Amaral.
The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DTP vaccine; Pertussis whole cell; Enzyme-linked immunosorbent assay; Intracerebral mouse protection test.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200001