Consulta: subjectFacets:"Enzymes"
Registros recuperados: 182
Data/hora: 16/06/2024 01:01:46

Effects of the Dietary Inclusion of Xylanase on the Performance and Jejunum Morphometry of Meat-Type Quails
Provedor de dados: 89
Autores: Grecco,ET; Marcato,SM; Oliveira,TMM; Stanquevis,CE; Grieser,DO; Perine,TP; Finco,EM; Benites,MI.
ABSTRACT The objective of this study was to evaluate the effects of the supplementation of two xylanase products to diets with reduced metabolizable energy fed to meat-type quails during the starter phase (1-14 days). A completely randomized experimental design in a 2 x 3 + 1 factorial arrangement two reduced metabolizable energy (ME) diets, inclusion or not of xylanase, and a control diet with no enzyme addition) was applied, totaling seven treatments with five replicates of 45 quails each. At 14 days of age, jejunum segments were collected for morphometry evaluation. No interaction between the studied factors were detected for performance and jejunal morphometry parameters. Body weight, weight gain, feed intake and feed conversion were not influenced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbohydrases; Energy; Enzymes; Intestine; Quail production.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400313

The Effect of Olive Cake, with or Without Enzymes Supplementation, on Growth Performance, Carcass Characteristics, Lymphoid Organs and Lipid Metabolism of Broiler Chickens
Provedor de dados: 89
Autores: Al-Harthi,MA.
ABSTRACT An experiment was carried out to investigate the effect of using olive cake (OC) in broiler diets, when it is supplemented with multi-enzymes or phytase enzyme. The OC was included in isocaloric, isonitorgneous diets at 5 and 10% levels and fed to broilers from 1-28 days of age. Experimental diets were fed with or without either of the two enzymes: galzym or phytase. This resulted in 3 OC levels (0, 5, 10%) × 3 enzyme supplementations (no enzyme, galzym enzyme, phytase enzyme). This included nine treatments, and each treatment was replicated eight times with seven broiler chickens each. Feed intake, feed conversion ratio, body weight gain, survival rate, dressing, inner and immune organ´s weights (compared to live body weight); and blood lipids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Enzymes; Olive cake; Carcasses parameters; Lymphoid organs and lipid metabolism constituents.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500083

Performance, Carcass Traits, Biochemical and Hematological Profile, Ileal Microbiota and Nutrient Metabolizability in Broilers Fed Diets Containing Cell Wall of Saccharomyces Cerevisiae and Piperine
Provedor de dados: 89
Autores: Trindade,BS; Lima,CAR; Cardoso,VS; Direito,GM; Machado,NJB; Souza,MMS; Curvello,FA; Corrêa,GSS.
ABSTRACT The objective of this study was to evaluate the inclusion of cell wall of Saccharomyces cerevisiae (CWSc) and piperine in broiler rations and their effects on performance, carcass traits, blood parameters, ileal microbiota and nutrient digestibility. A randomized block design with five treatments and six replicates of 10 birds was used, totaling 300 chickens. The treatments consisted of: control ration (CR); CR + avilamycin (10 mg / kg); CR + CWSc (2.0 g / kg); CR + piperine (60 mg / kg); and CR + CWSc (2.0 g / kg) + piperine (60 mg / kg). The use of isolated piperine resulted in greater weight gain from 9 to 40 days of age (2505g). The additives CWSc and piperine conjugates influenced the lower coliform count in the ceca (4.45 CFU / g) and caused...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Additive; Coliforms; Enzymes; Hematocrit.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300322

Nutritional Balance of broilers fed diets containing two calcium levels and supplemented with different phytase levels
Provedor de dados: 89
Autores: Donato,DCZ; Ribeiro,P de AP; Magalhães,JD; Polycarpo,G do V; Garcia,PDSR; Burbarelli,MF de C; Albuquerque,R de.
The objective of this study was to evaluate the effect of diets containing different calcium and phytase levels on the nutritional balance of broilers.A total of 108 male AG Ross 308 broilers were used in each of the replicates phases used in the study: starter (1-21 days), grower (29-35 days) and finisher (36-42 days). A completely randomized experimental design in a 3 x 2 factorial arrangement, with three phytase levels (0, 600 or 1200 FTU/kg) and two calcium levels (0.94 and 0.66%; 0.84 and 0.59%; e 0.78 and 0.54% in the starter, grower, and finisher phases, respectively), totaling six treatments with six replicates each.The experimental feeds also contained reduced available phosphorus levels and minimum crude protein level.The method of total excreta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Calcium; Broilers.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000400010

Laying Performance and Egg Quality of Japanese Quails Fed Diets Containing Castor Meal and Enzyme Complex
Provedor de dados: 89
Autores: Ludke,MCMM; Pimentel,ACS; Ludke,JV; Silva,JCNS; Rabello,CBV; Santos,JS.
ABSTRACT The objective of this study was to evaluate the effect of diets formulated with corn plus soybean meal (CSM) or with 21% autoclaved castor meal (ACM), with the addition of two enzyme complexes (EC1 and EC2), on the performance and egg quality of laying quail. Two hundred and sixteen quails were selected by egg production and weight uniformity and distributed in a completely randomized design with six treatments (in a 2 × 3 cross-factorial arrangement) and six replicates with six birds each. Three CSM and three ACM diets supplemented withEC1, EC2, or unsupplemented were established. The trial lasted three cycles of 21 days. Feed intake, laying rate, egg mass, feed conversion per egg mass, and feed conversion per dozen eggs did not differ. The use...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autoclaved castor meal; Enzymes; Laying quails; Protein source.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400781

Microbial alterations of the soil influenced by induced compaction
Provedor de dados: 90
Autores: Pupin,Breno; Freddi,Onã da Silva; Nahas,Ely.
Compaction is one of the most destructive factors of soil quality, however the effects on the microbial community and enzyme activity have not been investigated in detail so far. The objective of this study was to evaluate the effects of soil compaction caused by the traffic of agricultural machines on the soil microbial community and its enzyme activity. Six compaction levels were induced by tractors with different weights driving over a Eutrustox soil and the final density was measured. Soil samples were collected after corn from the layers 0-0.10 and 0.10-0.20 m. The compaction effect on all studied properties was evident. Total bacteria counts were reduced significantly (by 22-30 %) and by 38-41 % of nitrifying bacteria in the soil with highest bulk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microorganisms; Enzymes; Corn.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500014

Antioxidant compoundsand total antioxidant activity in fruits of acerola from cv. Flor Branca, Florida Sweet and BRS 366
Provedor de dados: 94
Autores: Souza,Kellina Oliveira de; Moura,Carlos Farley Herbster; Brito,Edy Sousa de; Miranda,Maria Raquel Alcântara de.
Information on antioxidant properties at different ontological stages may help producers and food technologists to identify which cultivar and/or maturity stage are most adequate for their need, therefore this work aimed to study the changes in the antioxidant metabolism during acerola development. Fruit from cv. Flor Branca, BRS366 and Florida Sweet were harvested at different stages: immature green colored (I), physiologically mature with green color and maximum size (II), breaker (III) and full red ripe (IV). After harvest, fruits were selected, divided into four replications with 500 g each and evaluated regarding their titratable acidity, pH, soluble solids, total soluble sugar, vitamin C, polyphenol, anthocyanin, yellow flavonoid, total antioxidant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malpighia emarginata; Development; Vitamin C; Enzymes; Polyphenols.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452014000200004

Feed efficiency in Mangalarga Marchador foals fed diet supplemented with probiotics or phytase
Provedor de dados: 96
Autores: Moura,Raquel Silva de; Saliba,Eloísa de Oliveira Simões; Almeida,Fernando Queiroz de; Lana,Ângela Maria Quintão; Silva,Vinícius Pimentel; Rezende,Adalgiza Souza Carneiro de.
The objective was to assess the effect of probiotic or phytase supplementation on dry matter intake (DMI), average daily gain (ADG) and feed conversion (FC) in 24 Mangalarga Marchador foals (16 males, 8 females), with 241.6 ± 23.2 days of age, and 216.7 ± 27.8 kg of body weight (BW). The study lasted 28 days, the first 23 days for diet and handling adaptation, and the last five days for feces collection. The basal diet consisted of Cynodon pasture and pelleted concentrate (1.2 kg/100 kg BW), as well as water and mineral salt ad libitum. Treatments groups were assigned as follows: basal diet (control); basal diet + 5 g/day of live yeast culture (10(9) CFU/g of S. cerevisiae); basal diet + 2 g/day of live bacteria (10(8) CFU/g of B. bifidum, E. faecium, L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Horses; Lactobacillus; LIPE®; Pasture intake; Saccharomyces cerevisiae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000600011

Enzyme complex at different levels in diets with enriched ingredients for commercial laying hens
Provedor de dados: 96
Autores: Silva,Jaqueline de Cássia Ramos da; Rabello,Carlos Bôa-Viagem; Ludke,Maria do Carmo Mohaupt Marques; Lopes,Cláudia da Costa; Arruda,Emmanuele Maria Florêncio de; Lopes,Elainy Cristina; Oliveira,Camilla Roana Costa de; Oliveira,Cláudio José Parro de.
ABSTRACT The objective of this study was to evaluate levels of inclusion of an enzyme complex (EC) in corn- and soybean meal-based diets for laying hens on the digestibility of nutrients and energy. A metabolism trial was conducted using 75 Dekalb Brown laying hens at 26 weeks of age, which were distributed into five treatments with five replicates in a completely randomized design. The ingredients used in the diets received an additional (enriched) 3% methionine, lysine, cysteine, threonine, tryptophan, and metabolizable energy and 33.3% phosphorus. Treatments consisted of diets including 0, 150, 200, 250, or 300 mg.kg‒1 EC. We determined apparent metabolizable energy (AME); nitrogen-corrected AME (AMEn); apparent metabolizability coefficients of dry...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Digestibility; Enzymes; Hens; Nutrients.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100503

Xylanase for meat-type quails from 15 to 35 days old
Provedor de dados: 96
Autores: Grecco,Érica Travaini; Marcato,Simara Márcia; Oliveira-Bruxel,Taciana Maria de; Stanquevis,Caroline Espejo; Grieser,Daiane de Oliveira; Finco,Eline Maria; Zancanela,Vittor; Ferreira,Mariana Fátima Zanon.
ABSTRACT The objective of this study was to evaluate the effect of the supplementation of two xylanases in the diet of meat-type quail on performance, morphology of the intestinal mucosa, metabolization of nutrients, and carcass yield. The experimental design was a completely randomized 2×3+1 factorial arrangement [two metabolisable energy reductions (70 and 140 kcal/kg), with or without the inclusion of two xylanases (A and B), plus a control treatment without xylanase), totalling seven treatments with five replicates and 42 quail each. Total excreta were collected at 28 days of age to determine the metabolisable coefficients of dry matter, crude protein (CP), neutral detergent fibre (NDF), and gross energy (GE). There was no interaction between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carbohydrases; Carcass yield; Energy; Enzymes; Quail production.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100509

Effects of adding dried distillers grains with solubles (DDGS) to dog diets supplemented with xylanase and protease
Provedor de dados: 96
Autores: Risolia,Larissa Wünsche; Sabchuk,Tabyta Tamara; Murakami,Fabiane Yukiko; Félix,Ananda Portella; Maiorka,Alex; Oliveira,Simone Gisele de.
ABSTRACT This study aimed to evaluate the effects of including 200 g kg −1 dried distillers grains with solubles (DDGS) to xylanase- and protease-supplemented diets for dogs on kibble properties, digestibility, fecal characteristics, and palatability. Experimental diets consisted of: 0 g kg −1 DDGS without enzymes (0WE), 0 g kg −1 DDGS with xylanase and protease (0XP), 200 g kg −1 DDGS without enzymes (200WE), 200 g kg −1 DDGS with xylanase (200X), 200 g kg −1 DDGS with protease (200P), and 200 g kg −1 DDGS with xylanase and protease (200XP). Kibbles were evaluated for density, extruded size, expansion index, hardness, and uniformity. Six beagle dogs were distributed in a 6×6 Latin square design for analysis of digestibility and fecal characteristics. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Digestibility; Enzymes; Fecal characteristics; Palatability; Short-chain fatty acids.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100528

Effects of lines and inoculants on nutritive value and production costs of triticale silages
Provedor de dados: 96
Autores: Sucu,Ekin; Çifci,Esra Aydoğan.
ABSTRACT The current study was undertaken to investigate the ensilage characteristics in triticale lines treated by inoculants and their interaction on fermentation metabolites and rumen degradability. Costs were estimated for growing and feeding whole-crop triticale lines for animal production. Triticale hybrids were harvested at the dough stage of maturity (38% dry matter, DM). Plants were chopped approximately 2 cm after harvest and then treated with inoculants and were ensiled in 1.5-L mini laboratory silos. Two lactic acid bacterial inoculants with enzymes (LAB+enzymes I: Pediococcus acidilactici, Lactobacillus plantarum, and Streptococcus faecium with cellulase, hemicellulase, pentosanase, and amylase; LAB+enzyme II: P. acidilactici, L. plantarum,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cost; Degradability; Enzymes; Fermentation; Lactic acid bacteria; Triticale lines.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000700355

What mineral and vitamin levels to recommend in swine diets?
Provedor de dados: 96
Autores: Gaudré,Didier; Quiniou,Nathalie.
Minerals, including trace-elements, and vitamins are currently introduced into the pig diet in order to obtain suitable performances for the modern pig genotype bred in confinement. Most of the mineral and vitamin contents of cereals and usual protein compounds are characterized by their poor and variable availability. Supplemental diets are then usually needed. This paper, based on literature data, reviews the pig requirement for minerals and vitamins in order to ensure economic and profitable performance. Recent research has focused on phosphorus due to environmental considerations. The widespread use of microbial phytase has decreased the need for inorganic phosphates. Cereals are particularly deficient in calcium and sodium, consequently limestone and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environment; Enzymes; Feeding; Meat quality; Modern genotype; Nutritional requirements.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300019

Animal performance and nutrient digestibility of feedlot steers fed a diet supplemented with a mixture of direct-fed microbials and digestive enzymes
Provedor de dados: 96
Autores: Encinas,Christian Michael Alvarez; Villalobos,Guillermo Villalobos; Viveros,Joel Domínguez; Flores,Gabriela Corral; Almora,Enrique Alvarez; Rangel,Francisco Castillo.
ABSTRACT The objective of this study was to evaluate the effect of a mixture of direct-fed microbials (DFM), yeast, and digestive enzymes on animal performance in feedlot cattle, as well as digestibility when finishing feedlot cattle. Thirty crossbreed (Charolais × Beefmaster) steers averaging 15 months old and 321.83±3.73 kg of initial body weight were used. Animals were randomly assigned to one of two treatment groups: control (basal diet) and a mixture of DFM, yeast, and digestive enzymes (basal diet + 30 g of the mixture). Each group contained fifteen experimental units. Animals were fed individually twice a day, and dry matter intake (DMI) was recorded daily. Body weights were recorded initially and subsequently at 28-day intervals for a total of 140...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Finishing; Probiotics; Saccharomyces cerevisiae; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100615

Blood biochemical parameters of Brazilian sport horses under training in tropical climate
Provedor de dados: 96
Autores: Padilha,Felipe Gomes Ferreira; Dimache,Luana Avila Giorgia; Almeida,Fernando Queiroz de; Ferreira,Ana Maria Reis.
ABSTRACT This study aimed to provide baseline data for the serum biochemical parameters of Brazilian sport horses undergoing physical training for eventing to be used as a practical guide. A total of 139 blood samples were collected by vacuum venipuncture of the jugular vein from healthy horses in training. Tubes without anticoagulant were used for biochemical analysis. Means and standard deviations were determined for aspartate aminotransferase (AST) (306.27±84.66 U/L), creatine kinase (CK) (181.06±80.38 U/L), urea (29.51±5.82 mg/dL), creatinine (1.44±0.20 mg/dL), gamma glutamyl transferase (GGT) (14.58±6.55 U/L), calcium (13.22±0.59 mg/dL), and albumin (2.86±0.16 g/dL). The specific reference ranges were: AST, 197-454 U/L; CK, 116-290 U/L; urea, 22-42...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enzymes; Horses; Serum biochemistry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000800678

Effects of enzyme complex SSF (solid state fermentation) in pellet diets for Nile tilapia
Provedor de dados: 96
Autores: Moura,Guilherme de Souza; Lanna,Eduardo Arruda Teixeira; Filer,Keith; Falkoski,Daniel Luciano; Donzele,Juarez Lopes; Oliveira,Maria Goreti de Almeida; Rezende,Sebastião Tavares de.
The effects of enzyme complex SSF (solid state fermentation) on growth performance and the availability of sucrose and monosaccharides in the chyme of Nile were involved. The study included 360 fish (70g±4.43) in a completely randomized design with six dietary treatments (0, 50, 100, 150, 200 and 250 ppm of SSF) arranged in six replicates, with 10 fish per replicate. Every 15 days, one tilapia of each experimental unit was sacrificed for analyses of carbohydrate in the chyme. On day 60 of the experiment, the performance parameters were measured. There was a linear effect according to treatment for final weight and weight gain. For the other performance parameters, there were no differences. There was quadratic effect for sucrose and glucose in function of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Enzymes; Fish nutrition; HPLC; Oreochromis niloticus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000001

Determination of nutrient and energy values of cottonseed meal supplemented or not with phytase and protease for broiler chicks
Provedor de dados: 96
Autores: Tavares-Samay,Alcilene Maria Andrade; Dutra Junior,Wilson Moreira; Palhares,Liliane Olímpio; Lopes,Cláudia da Costa; Rabello,Carlos Bôa-Viagem; Coelho,Andrew Henrique da Silva Cavalcanti.
ABSTRACT The objective of this study was to determine the nutritional and energy compositions of cottonseed meal (CM), with or without enzyme supplementation, for broilers at different ages. A total of 672 male Cobb 500 chickens were distributed into four metabolism and ileal-digestibility trials. The experimental design was completely randomized, with four treatments and six replicates. The following treatments were tested: a corn- and soybean meal-based control diet without enzymes; control diet with enzyme addition; control diet with 25% replaced by CM; and control diet with enzyme addition and 25% replaced by CM. The following variables were investigated: apparent metabolizable energy (AME); nitrogen-corrected AME (AMEn); apparent metabolizability...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Alternative feedstuffs; Amino acids; Enzymes; Metabolizable energy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100521

β-galactosidases from cowpea stems: properties and gene expression under conditions of salt stress
Provedor de dados: 124
Autores: Sudério,Fabrício Bonfim; Gomes Filho,Enéas; Costa,José Hélio; Enéas Filho,Joaquim.
The aim of this work was to evaluate the properties and gene expression of isoforms of β-galactosidase, isolated from the stems of seedlings of the cowpea cv. Pitiuba, at different stages of development and sown in the absence and presence of NaCl. Seeds were sown onto sheets of filter paper soaked in distilled water (control) and a 100 mM NaCl solution. The plants were collected at the III (ED III) and VIII (ED VIII) stages of development. Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied. The three isoforms were stable up to 50 °C. The thermal inactivation curves of the enzymes as a function of time were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vigna unguiculata; Enzymes; Purification.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000400018

Xylanase and β-glucanase in diets for Japanese laying quails
Provedor de dados: 124
Autores: Gouveia,Alison Batista Vieira Silva; Souza,Christiane Silva; Santos,Fabiana Ramos dos; Minafra-Rezende,Cíntia Silva; Minafra,Cibele Silva.
RESUMO As rações dos animais monogástricos no Brasil constituem-se de milho e farelo de soja. Dentre os entraves do uso de grãos de cereais nas dietas destaca-se a presença de polissacarídeos não amiláceos, constituintes de baixa digestibilidade, não digeridos pelas aves. Desse modo, faz-se importância estudar ingredientes não convencionais e uso de aditivos, tais como as enzimas nas rações. Objetivou-se avaliar a produtividade e o metabolismo de codornas japonesas em postura, alimentadas com rações à base de milho ou sorgo contendo ou não xilanase e β-glucanase. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2x2, sendo quatro tratamentos, com cinco repetições e sete aves em cada, perfazendo 140 animais. Os tratamentos foram:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coturnix coturnix japonica; Egg quality; Enzymes; Performance; Seedor Index.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000300407

VCP1 PROTEASE DETECTION IN Pochonia chlamydosporia var. CATENULATA STRAIN IMI SD 187
Provedor de dados: 100
Autores: Peteira-Delgado,B; Hidalgo-Díaz,L.
The mode of action of Pochonia as a biological control agent is the penetration by means of the hyphae into the target nematode eggs. The egg shell of nematodes has an outer vitelline membrane composed mainly by proteins. Thus, hyphal penetration is the result of a physic pressure and the specific hydrolytic activity of some enzymes such as proteases and chitinases. The most important protease studied in Pochonia chlamydosporia var. chlamydosporia is VCP1. The aim of this work was to detect and characterize the protease VCP1 in a strain of P. chlamydosporia var. catenulata, a potential biological control agent for root-knot nematodes. The specific activity of this protease was tested on different culture media supplemented with protein inductors using...
Tipo: Journal article
Palavras-chave: Nematode parasites; RFLP; Enzymes; Protease.
Ano: 2009
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522009000300006

Enzymes of Significance to the Restoration of Polluted Systems: Traditional and Advanced Approaches
Provedor de dados: 127
Autores: Gianfreda,L.
Tipo: Journal article
Palavras-chave: Xenobiotics; Enzymes; Soil bioremediation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400004

Interaction with Soil Constituents Determines the Environmental Impact of Proteins
Provedor de dados: 127
Autores: Quiquampoix,H.
Tipo: Journal article
Palavras-chave: Proteins; Enzymes; Clay-humic complexes.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400012

Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost
Provedor de dados: 63
Autores: Chang,Chen-Chin; Ng,Chang-Chai; Wang,Chung-Yi; Shyu,Yuan-Tay.
Plant wastes present a high cellulose content, which is an ideal organic material for composting. Five strains of thermophiles from processed Brassica waste were isolated, and the hydrolytic activity on various cellulosic biomass substrata and their temperature profiles were determined. 16S rRNA sequencing identified these strains as Thermoactinomyces and Bacillus spp. Maximal cellulase activity corresponded to 2.3 U mL-1 of enzyme. The application of these strains on Brassica rapa residues demonstrates increased total nitrogen content). TA-3, a Thermoactinomycetes sp. strain, performs best among all inoculants, increasing the nitrogen content from 0.74 to 0.91%, and decreasing the carbon content from 15.4 to 12.2%, showing its high efficiency and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulose degrading; Enzymes; Vegetable wastes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300004

Invertase and sucrose synthase activities in coffee plants sprayed with sucrose solution
Provedor de dados: 63
Autores: Silva,José Carlos da; Alves,José Donizeti; Alvarenga,Amauri Alves de; Magalhães,Marcelo Murad; Livramento,Dárlan Einstein do; Fries,Daniela Deitos.
One management practice of which the efficiency has not yet been scientifically tested is spraying coffee plants with diluted sucrose solutions as a source of carbon for the plant. This paper evaluates the effect of foliar spraying with sugar on the endogenous level of carbohydrates and on the activities of invertase and sucrose synthase in coffee (Coffea arabica L.) seedlings with reduced (low) and high (normal) levels of carbon reserve. The concentrations used were 0.5 and 1.0% sucrose, and water as a control. The use of sucrose at 1.0% caused an increase in the concentration of total soluble sugars in depauperate plants, as well as increased the activity of the following enzymes: cell wall and vacuole acid invertase, neutral cytosol invertase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea arabica L.; Sugar; Spray; Enzymes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200006

Microbial enzymatic activity and thermal effect in a tropical soil treated with organic materials
Provedor de dados: 63
Autores: Cenciani,Karina; Freitas,Sueli dos Santos; Critter,Silvana Auxiliadora Missola; Airoldi,Cláudio.
Bacteria and fungi are the most active decomposers of organic materials in soil. They directly affect plant nutrient availability, and chemical and physical properties of soils. This investigation aimed at quantifying the effect of several organic materials on microbial activity of a Rhodic Eutrudox. Soil samples were incubated over a period of 91 days with the following organic materials: cattle manure (CM), earthworm humus (HM), and city sewage sludge from Barueri (BA) and Franca (FR). The activities of cellulase, protease and urease enzymes, the soil microbial carbon content (by fumigation-extraction method) and the exothermal effect were evaluated. Experimental design was randomized and arranged as factorial scheme five treatments x seven samplings...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Biomass carbon; Organic materials; Soil microorganisms; Microcalorimetry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000600016

Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides.
Provedor de dados: 119
Autores: CECHIN, J.; VARGAS, L.; AGOSTINETTO, D.; LAMEGO, F. P.; MARIANI, F.; DAL MAGRO, T.
Tipo: Separatas
Palavras-chave: Enzima; Mutação hereditária; Metabolismo vegetal; Nabo; Herbicida; Raphanus Sativus.; Enzymes; Mutation; Turnips; Herbicides.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1070083

Improving enzymatic saccharification of peach palm (Bactris gasipaes) wastes via biological pretreatment with Pleurotus ostreatus.
Provedor de dados: 119
Autores: SPACKI, K. de C.; NOVI, D. M. P.; OLIVEIRA-JUNIOR, V. A. de; DURIGON, D. C.; FRAGA, F. C.; SANTOS, L. F. O. dos; HELM, C. V.; LIMA, E. A. de; PERALTA, R. A.; MOREIRA, R. de F. P. M.; CORRÊA, R. C. G.; BRACHT, A.; PERALTA, R. M.
Abstract. The white-rot fungus Pleurotus ostreatus was used for biological pretreatment of peach palm (Bactris gasipaes) lignocellulosic wastes. Non-treated and treated B. gasipaes inner sheaths and peel were submitted to hydrolysis using a commercial cellulase preparation from T. reesei. The amounts of total reducing sugars and glucose obtained from the 30 d-pretreated inner sheaths were seven and five times higher, respectively, than those obtained from the inner sheaths without pretreatment. No such improvement was found, however, in the pretreated B. gasipaes peels. Scanning electronic microscopy of the lignocellulosic fibers was performed to verify the structural changes caused by the biological pretreatments. Upon the biological pretreatment, the...
Tipo: Artigo de periódico
Palavras-chave: Circular economy; Renewable energy; Upcycling; Bactris Gasipaes; Pleurotus ostreatus; Enzymes.
Ano: 2023
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159167

Caracterização de uma amilase de sementes de Libidibia ferrea (Mart. ex. Tul.).
Provedor de dados: 119
Autores: LIMA, T. C.; ALVES, J. P. de A.; BRITO, M. S. de; FIGUEIREDO, M. F.; SALLES, H. O.; ANDRADE, L. B. da S.
Caracterizar uma amilase de extratos aquosos de sementes de L. ferrea quanto à estabilidade térmica e massa molecular.
Tipo: Parte de livro
Palavras-chave: Glicosidase; Pau-ferro; Libidibia ferrea; Potencial biotecnológico; Indigenous organisms.; Jucá; Caesalpinia ferrea; Espécie nativa; Leguminosa; Proteína; Enzima; Amido; Enzymes; Amylases; Starch; Glycosidases; Proteinases.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/973728

Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil.
Provedor de dados: 119
Autores: GALÃO, O. F.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; LEITE, R. S.; CLAUS, T.; VISENTAINER, J. V.
Diferenças em níveis de inibidor de tripsina Kunitz e ácido fítico foram determinadas para cultivares de soja convencionais e transgênicas cultivadas em Londrina e Ponta Grossa, Sul do Brasil. Os níveis de inibidor de tripsina na região de Londrina e Ponta Grossa para variedades convencionais variaram de 14,56 mg g-1 (BRS 267) a 20,40 mg g-1 (BRS 261) e de 13,51 mg g-1 (BRS 232) a 19,65 mg g-1 (BRS 268), respectivamente, enquanto para as variedades transgênicas foram encontrados valores de 14,16 mg g-1 (BRS 242 RR) a 18,21 mg g-1 (BRS 255 RR) para Londrina e 13,68 mg g-1 (BRS 244 RR) a 18,73 mg g-1 (BRS 256 RR) para Ponta Grossa. A média de teor de ácido fítico em variedades convencionais e transgênicas de Londrina teve variação de 2,05 a 1,78 mg g-1,...
Tipo: Artigo de periódico
Palavras-chave: Soja convencional; Soja transgêncica; Enzimas; Melhoramento genético; Fatores antinutricionais; Conventional soybean; Genetically modified soybean.; Antinutritional factors; Enzymes; Genetic improvement.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1007382

The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighia emarginata) purée.
Provedor de dados: 119
Autores: OLIVEIRA, L. S.; RUFINO, M. do S. M.; MOURA, C. F. H.; CAVALCANTI, F. R.; ALVES, R. E.; MIRANDA, M. R. A. de.
Tipo: Separatas
Palavras-chave: Vitamin C; Postharvest; Frozen.; Enzymes; Pulp.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/901053

The potential of [beta]-glucosidases for aroma and flavor improvement in the food indstry.
Provedor de dados: 119
Autores: SOUTO, B. de M.; BARBOSA, M. F.; SALES, R. M. M.; MOURA, S. C. de; ARAUJO, A. de R. B.; QUIRINO, B. F.
Tipo: Artigo de periódico
Palavras-chave: Indústria Alimentícia; Aroma; Enzima; Food industry; Enzymes; Glucosidases; Flavor; Beverage industry.
Ano: 2023
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156460

Immunodetection of angiotensin converting enzyme in Nelore sperm.
Provedor de dados: 119
Autores: ALMEIDA, R. G. de; FARIÁ, F. J. C.; SILVA, J. C. B.; SAMPAIO, B. F. B.; SANTOS, M. D. dos; FERNANDES, C. A. C.; COSTA, D. S.
The study was designed to perform immunodetection in spermatozoa and seminal plasma, and immunolocalization in spermatozoa of tACE before and after freezing semen of Nelore bulls. Semen samples from 10 sexually mature bulls were used. Objetivou-se com esta pesquisa caracterizar a tACE antes e após o congelamento do sêmen de touros Nelore.
Tipo: Separatas
Palavras-chave: Enzima; Criopreservação; Sêmen.; Enzymes; Cryopreservation.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061162

Tolerance of alfafa varieties subjected to salinity.
Provedor de dados: 119
Autores: SILVA, R. M. e; SIMOES, W. L.; GUIMARÃES, M. J. M.; BARROS, J. R. A.; BARBOSA, K. V. F.; ANGELOTTI, F.
Saline water can be an alternative for the production of salt-tolerant forages. Thus, the objective was to evaluate the tolerance of alfalfa varieties to salinity, aiming at their inclusion in production systems that use irrigation water with high levels of salt or salinized soils. The experiment was conducted in a greenhouse, in a 3x5x3 factorial, three alfalfa varieties (LE-N4, Crioula and DK-192), five salinity levels (0; 3.5; 5.0; 7.0; 10. 5 and 14.0 dS m-1) and three cuts of the aerial part, with four replications. For irrigation management, lysimeters were installed. Fresh and dry plant biomass, chlorophyll, water use efficiency and enzymatic activity were analyzed. Increased salinity reduced fresh and dry biomass of shoots and roots, chlorophyll...
Tipo: Artigo de periódico
Palavras-chave: Forrageiras tolerantes ao sal; Estresse oxidativo; Estresse salino; Biomassa fresca e seca; Alfafa; Água Salina; Salinidade; Biomassa; Enzima; Medicago Sativa; Medicago sativa subsp. falcata; Saline water; Biomass; Enzymes; Oxidative stress.
Ano: 2023
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1158230

Potencial biotecnológico de actinomicetos para produção de amilase.
Provedor de dados: 119
Autores: OLIVEIRA, J. C. M. D. de; BOMBA, J. P.; SOUSA, J. C. da C. de; MELO, I. G.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; MARRIEL, I. E.
FertBio 2016.
Tipo: Parte de livro
Palavras-chave: Enzima; Streptomyces; Kitasatospora; Enzymes.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056147

Different phytase levels and energy densities in broiler diets on performance, nutrient digestibility, and bone integrity from 28 to 35 days of age.
Provedor de dados: 119
Autores: DALLMANN, H. M.; AVILA, V. S. de; KRABBE, E. L.; SUREK, D.; BEDENDO, G. C.; TOLEDO, T. S.; DALLMANN, P. R.; ROLL, A. A. P.; ROLL, V. F. B.; RUTZ, F.
Abstract: The study was carried out with the objective of evaluating the effects of using phytase levels at different energy densities in the diet of broilers, from 28 to 35 days of age. The experimental diets contained increasing levels of apparent metabolizable energy corrected for nitrogen balance and different levels of phytase. Growth performance variables, nutrient digestibility, as well as variables related to bone integrity were examined. Diets containing 500 FTU/kg in combination with 3150kcal.kg-1 of AMEn resulted in better growth performance. Supplementation with 500 and 1000 FTU/kg in the diets provide better coefficients of apparent and ileal digestibility of calcium and phosphorus, but negatively influence the deposition of calcium,...
Tipo: Artigo de periódico
Palavras-chave: Phytate; Fitato; Frango de Corte; Aditivo; Tíbia; Enzima; Fósforo; Broiler chickens; Additives; Enzymes; Phosphorus.
Ano: 2023
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153135

Effects of in vitro drought stress on growth, proline accumulation and antioxidant defense in sugarcane.
Provedor de dados: 119
Autores: OLIVEIRA, L. A. R. de; CARDOSO, M. N.; OLIVEIRA, A. C. A. de; MACHADO, C. de A.; CARDOSO, B. T.; MUNIZ, A. V. C. da S.; LEDO, A. da S.
Drought is the most limiting environmental factor to crop productivity and presents a great variability in the degree of tolerance among and within species, among varieties. The aim of this study was to characterize sugarcane accessions regarding tolerance to water stress during in vitro cultivation based on changes in biometric, physiological and biochemical characteristics, within species and among species, to support future breeding programs. Adventitious shoots of five sugarcane accessions: Saccharum robustum, Saccharum spontaneum and Saccharum officinarum species, cultivated in Murashige and Skoog medium supplemented with 2% sucrose and 4 g/l Phytagel were used in five water potentials, 0, -0.3, -0.6, -0.9, -1.2 MPa, induced by mannitol. Survival,...
Tipo: Artigo de periódico
Palavras-chave: Cana de Açúcar; Prolina; Absorção de Água; Enzima; Antioxidante; Cane sugar; Drought; Proline; Enzymes; Antioxidants.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106068

Identificação de enzimas vegetais com atividade coagulante sobre o leite para possível utilização na fabricação de queijos de cabra.
Provedor de dados: 119
Autores: RODRIGUES, R. C.; LAGUNA, L. E.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Caprino; Leite de cabra; Coagulação; Extrato vegetal; Queijo; Tecnologia de alimento; Enzima; Goat milk; Plant extracts; Coagulation; Goat cheese; Enzymes.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/531663

Compostos antioxidantes e atividade antioxidante total em frutos das variedades Flor Branca, Florida Sweet e BRS 366 de aceroleira.
Provedor de dados: 119
Autores: SOUZA, K. O.; MOURA, C. F. H.; BRITO, E. S. de; MIRANDA, M. R. A. de.
Tipo: Artigo de periódico
Palavras-chave: Desenvolvimento; Enzimas; Polifenóis; Development; Vitamin C.; Vitamina C.; Malpighia emarginata; Enzymes; Polyphenols.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/999371

Mecanismos de tolerancia da variedade de milho Saracura (BRS 4154) ao alagamento.
Provedor de dados: 119
Autores: ALVES, J. D.; MAGALHAES, M. M.; GOULART, P. de F. P.; DANTAS, B. F.; GOUVEA, J. A. de; PURCINO, R. P.; MAGALHAES, P. C.; FRIES, D. D.; LIVRAMENTO, D. E. do; MEYER, L. E.; SEIFFERT, M.; SILVEIRA, T.
Tipo: Artigo de periódico
Palavras-chave: Hipoxia; Parede celular; Maize; Variety; Cell wall; Cálcio; Enzima; Milho; Variedade; Calcium; Enzymes.
Ano: 2002
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/134585

Avaliação do hemograma e das funções hepática e renal de cabras leiteiras alimentadas com farelo de mamona destoxificado.
Provedor de dados: 119
Autores: FERNANDES, M. F.; MEDEIROS, A. N. de; BOMFIM, M. A. D.; QUEIROGA, R. de C. R. do E.; PINHEIRO, R. R.; SANTIAGO, L. B.
O objetivo deste estudo foi avaliar a eficácia in vivo do processo de destoxificação do farelo de mamona através da avaliação do hemograma e das funções hepática e renal de cabras leiteiras. Foram utilizadas 28 cabras da raça Anglo-Nubiana e Saanen, alojadas em baias individuais e distribuídas em um delineamento inteiramente casualizado com quatro níveis de substituição (0%, 33,3%, 66,6% e 100%) do farelo de soja pelo farelo de mamona destoxificado e sete repetições. O período experimental foi composto por 30 dias de adaptação à dieta e 5 dias para a coleta dos dados. O exame hematológico consistiu na determinação do volume globular, hemáceas, hemoglobina, volume globular médio, concentração hemoglobínica globular média, hemoglobina globular média,...
Tipo: Anais e Proceedings de eventos
Palavras-chave: Farelo de mamona; Função renal; Enzima hepática; Co-produto.; Caprino; Nutrição animal; Alimento alternativo; Alimento para animal; Ricinus communis; Mamona; Ricina; Resíduo; Subproduto.; Ricinus; Goats; Enzymes; Hematology; Biodiesel.
Ano: 2010
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/870294

Evaluation of nutrient excretion and retention in broilers submitted to different nutritional strategies.
Provedor de dados: 119
Autores: GRAÑA, A. L.; TAVERNARI, F. de C.; LELIS, G. R.; ALBINO, L. F. T.; ROSTAGNO, H. S.; GOMES, P. C.
Tipo: Artigo de periódico
Palavras-chave: Nutrição animal; Frango de corte; Fósforo; Enzima; Animal nutrition; Broiler chickens; Enzymes; Phosphorus.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/967181

Atividade enzimática na rizosfera de milho sob diferentes métodos de inoculação com azospirillum.
Provedor de dados: 119
Autores: REIS, D. P.; FONSECA, L. M. F.; DUARTE, E. S.; OLIVEIRA, C. M. R.; OLIVEIRA, C. A.; GUIMARAES, L. J. M.; MARRIEL, I. E.
Tipo: Parte de livro
Palavras-chave: Enzima; Microrganismo; Enzymes; Microorganisms.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001573

Enzimas em líquidos iônicos: Uma combinação promissora para um biodiesel limpo.
Provedor de dados: 119
Autores: MEDEIROS, G. A.; GONCALVES, S. B.; RODRIGUES, D. de S.; NETO, B. A. D.
Tipo: Separatas
Palavras-chave: Enzymes; Biodiesel; Ionic liquids.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/964947

Avaliações da parasitemia, hematócrito e níveis enzimáticos de bezerros Nelore (Bos indicus), inoculados com isolados de Babesia bigemina (Smith &amp; Kilborne, 1893), das Regiões Sul, Sudeste, Centro-Oeste, Nordeste e Norte do Brasil.
Provedor de dados: 119
Autores: VIEIRA, D.; MENDONCA, L.; KAHYAGAWA, A.; MADRUGA, C. R.; BICUDO, P. L.; SCHENK, M. A.; KESSLER, R.
Avaliaram-se parasitemia e os niveis de aspartato amino-transferase (AST), gama glutamil-transerase (GGT), glutamil desidrogenase (GLDH) e fosfatase alcalina (FA), de bezerros Nelore (Bos indicus) inoculados com isolados de Babesia bigemina das cinco regioes fisiograficas do Brasil. Constatou-se que os diferentes isolados desenvolveram baixa parasitemia nos animais experimentalmente inoculados e que nao houve variacao nos niveis das enzimas sericas estudadas.
Tipo: Artigo de periódico
Palavras-chave: Bovino de corte; Nelore; Brasil; Babesia Bigemina; Bezerro; Enzima; Parasitismo; Nellore; Beef cattle; Calves; Enzymes; Parasitism.
Ano: 2000
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/324312

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates.
Provedor de dados: 119
Autores: MELO, J. F. B.; LUNDSTEDT, L. M.; INOUE, L. A. K.; METÓN, I.; BAANANTE, I. V.; MORAES, G.
The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH) and glucose...
Tipo: Artigo de periódico
Palavras-chave: Enzima; Metabolismo; Peixe; Nutriente; Bagre; Proteina; Fish; Catfish; Enzymes; Metabolism; Fish nutrition.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058428

Caracterização das lipases obtidas por duas cepas de Aspergillus niger.
Provedor de dados: 119
Autores: SOUSA, F. do C. A. de; SOUZA, E. F. de; TERZI, S. da C.; BRIGIDA, A. I. S.; GOTTSCHALK, L. M. F.; PENHA, E. das M.
As lipases são enzimas que catalisam a hidrólise parcial ou total de triacilglicerois produzindo ácidos graxos livres, diacilglicerol, monoacilglicerol e glicerol. Este trabalho caracterizou a especificidade, a temperatura ótima e o pH ótimo das lipases obtidas por duas cepas de Aspergillus niger, sendo uma selvagem C e outra mutante 11T53A14. Os resultados mostram que os extratos enzimáticos apresentam especificidade diferentes, sendo a cepa selvagem mais específica para ácidos graxos de 8 carbonos e a cepa mutante inespecífica em relação ao tamanho do ácido graxo. As duas cepas apresentaram atividade em uma ampla faixa de pH, porém observou-se uma redução da atividade de mais de 50% em pH acima de 9,0. Em relação à temperatura, as lipases das duas cepas...
Tipo: Separatas
Palavras-chave: Enzima; Lípase; Ph; Temperatura.; Aspergillus; Enzymes.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053675

Efeito da insulação nos níveis enzimáticos de AST e ALT no plasma seminal de caprinos.
Provedor de dados: 119
Autores: PINHEIRO, R. R.; SALLES, H. O.; AZEVEDO, H. C.; SIMPLICIO, A. A.; SANTOS, D. O.; ANDRIOLI, A.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Caprino; Sêmen; Bioquímica; Enzima; Goats; Enzymes; Biochemistry.
Ano: 1993
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/515049

Identification of new Sphingomyelinases D in pathogenic fungi and other pathogenic organisms.
Provedor de dados: 119
Autores: DIAS-LOPES, C.; NESHICH, I. A. P.; NESHICH, G.; ORTEGA, J. M.; GRANIER, C.; CHÁVEZ-OLORTEGUI, C.; MOLINA, F.; FELICORI, L.
Sphingomyelinases D (SMases D) or dermonecrotic toxins are well characterized in Loxosceles spider venoms and have been described in some strains of pathogenic microorganisms, such as Corynebacterium sp. After spider bites, the SMase D molecules cause skin necrosis and occasional severe systemic manifestations, such as acute renal failure. In this paper, we identified new SMase D amino acid sequences from various organisms belonging to 24 distinct genera, of which, 19 are new. These SMases D share a conserved active site and a C-terminal motif. We suggest that the C-terminal tail is responsible for stabilizing the entire internal structure of the SMase D Tim barrel and that it can be considered an SMase D hallmark in combination with the amino acid...
Tipo: Artigo de periódico
Palavras-chave: Bioinformática; Esfingomielinas; Enzimas; Bioinformatics; Sphingomyelins; Enzymes.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/974829

Changes in enzymatic activity, technological quality and gamma-aminobutyric acid (GABA) content of wheat flour as affected by germination.
Provedor de dados: 119
Autores: BARANZELLI, J.; KRINGEL, D. H.; COLUSSI, R.; PAIVA, F. F.; ARANHA, B. C.; MIRANDA, M. Z. de; ZAVAREZE, E. da R.; DIAS, A. R. G.
Tipo: Artigo de periódico
Palavras-chave: GABA; Trigo; Germinação; Enzima; Pão; Wheat; Germination; Enzymes; Breads.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092245

Improving cellulolytic enzyme production using sugarcane bagasse in a solid-state fermentation bioreactor.
Provedor de dados: 119
Autores: VITCOSQUE, G. L.; FARINAS, C. S.; FONSECA, R. F.; BERTUCCI NETO, V.
Tipo: Separatas
Palavras-chave: Xilanase; Endoglucanase; Xylanase; Cellulase; Enzimas; Bioetanol; Fermentação em estado sólido.; Fermentação; Celulase.; Solid state fermentation.; Enzymes; Bioethanol.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/901997

Enzymatic profile of actinobacteria across a desertification gradient in the brazilian Semiarid region.
Provedor de dados: 119
Autores: SANTOS, F. D. dos; SOUSA, J. B. de; SILVA, V. M. A.; BANDEIRA, L. L.; SILVA, V. B. da; FERNANDES JUNIOR, P. I.; MARTINS, S. C. S.; MARTINS, C. M.
This study aimed to determine the enzymatic profile for xylanase, amylase, cellulase and pectinase in areas with different levels of desertification, in order to investigate how this process influences the enzymatic variation of actinobacteria.
Tipo: Artigo de periódico
Palavras-chave: Enzimas Hidrolíticas; Serviços Ecossistêmicos; Semiárido; Desertificação; Cobertura Vegetal; Enzima; Vegetation cover; Enzymes; Ecosystem services.
Ano: 2024
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159842

Screening of protease-producing microorganisms from anaerobic biodigestion system of dairy cattle manure.
Provedor de dados: 119
Autores: SANTANA, L. A. R.; CUNHA, D. S.; TEODORO, L. da C.; OTENIO, M. H.; RODARTE, M.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Biodigestão; Bovino; Gado Leiteiro; Protease; Enzima; Enzymes; Screening.
Ano: 2022
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148432

Phosphatases and antioxidant enzymes as potential biochemical indicators of toxicity in aquatic non-target organisms.
Provedor de dados: 119
Autores: DANTZGER, D. D.; JONSSON, C. M.; AOYAMA, H.
Tipo: Separatas
Palavras-chave: Phosphatases; Antioxidant Enzymes; Biochemical Indicators; Antioxidants; Enzymes; Diflubenzuron.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010917

Efeito do uso de fitase em dietas de frangos de corte sobre a biodisponibilidade de minerais e aproveitamento de energia.
Provedor de dados: 119
Autores: AVILA, V. S. de; KRABBE, E. L.; KLEIN, C. H.; LOPES, L. dos S.; SANDRI, E. C.; MAIORKA, A.
Tipo: Separatas
Palavras-chave: Nutrição animal; Dieta; Ração; Frango de corte; Animal nutrition; Broiler feeding; Enzymes; Phytases.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/969559

Proposta conceitual de estudo para a integração e gerenciamento do uso de marcadores bioquímicos de contaminação ambiental.
Provedor de dados: 119
Autores: CASTRO, V. L. S. S. de; JONSSON, C. M.
Resumo: A agricultura sustentável prioriza o uso racional da terra e da biota. Para tanto, é necessária a compreensão da interação entre os insumos agrícolas e os ecossistemas. Porém, há carência de métodos de fácil acesso para a verificação de contaminação ambiental por muitos desses produtos, como os agrotóxicos e de suas misturas. A aplicação de métodos alternativos no monitoramento de áreas degradadas pode gerar vantagens econômicas quando comparados à implementação de protocolos de análise que utilizam métodos de alto custo que demandam maior tempo de análise. Uma vez que a saúde ambiental pode ser avaliada por meio de estudos com indicadores biológicos, a abordagem aqui apresentada objetiva discutir alguns aspectos a respeito do monitoramento de...
Tipo: Separatas
Palavras-chave: Monitoramento ambiental; Árvore de decisão; Enzimas; Indicadore biológico; Biomarcador; Agrotóxico; Impacto ambiental; Poluição ambiental; Pesticides; Environmental monitoring; Biomarkers; Enzymes.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/930565

Effect of temperature in Amylase and amyloglucosidase produced from english potato residue via fermentation in solid state.
Provedor de dados: 119
Autores: MELO, F. S.; ALVES, I. D. C.; BARBOSA, T. C. V.; CABRAL, L. M. C.; SANTOS, T. C.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Amyloglucosidase; Amylase; Enzymes; Fermentation.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122367

Antioxidant defenses of irrigated forage sorghum with saline aquaculture effluent.
Provedor de dados: 119
Autores: GUIMARÃES, M. J. M.; SIMOES, W. L.; CAMARA, T. de J. R.; SILVA, C. U. de C; WILLADINO, L. G.
The objective of this work was to evaluate the biomass production and antioxidant enzymatic system activity of irrigated forage sorghum with saline aquaculture effluent under different leaching fractions. The experiment was conducted in the Caatinga Experimental Field of the Embrapa Semiarido, in Petrolina, State of Pernambuco, Brazil. The experimental design was a complete randomized block in a split-plot arrangement with four replications, consisting of three forage sorghum varieties (Volumax, F305 and Sudan) and four leaching fractions (0, 5, 10 and 15%). The vegetal materials were collected when the plants were at the soft-dough stage. The biomass production and activity of the enzymes superoxide dismutase, catalase and ascorbate peroxidase were...
Tipo: Artigo de periódico
Palavras-chave: Enzimas; Estresse oxidativo; Sorghum.; Sorgo forrageiro; Irrigação; Água Salina; Sorghum Bicolor.; Enzymes; Oxidative stress.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089388

Eficiencia de uso de nitrogênio em sorgo: produtividade de grãos e atividade de enzimas de assimilação de carbono e nitrogênio.
Provedor de dados: 119
Autores: PURCINO, A. A. C.; ANDRADE, S. R. M.; SANTOS, F. G.; OLIVEIRA, A. C.; SILVA, M. R.
Tipo: Parte de livro
Palavras-chave: Enzimas.; Carbono; Nitrogenase; Microbiologia; Nitrogênio.; Enzymes; Carbon; Nitrogen.; Microbiology.
Ano: 1995
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/474604

Estabilidade térmica de celulases em extratos aquosos de sementes de Libidibia ferrea (Mart.) L.P. Queiroz.
Provedor de dados: 119
Autores: SILVA, L. J. da C.; OLIVEIRA, T. dos R.; SOUZA, E. M. de; LIMA, T. C.; SALLES, H. O.; ANDRADE, L. B. da S.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Libidibia ferrea; Estabilidade térmica; Cellulase.; Jucá; Celulase; Enzima; Semente; Extrato vegetal; Vegetable extracts.; Enzymes.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/975134

Atributos microbiológicos do solo de áreas mineradas em recuperação: I - atividade de urease e arginase.
Provedor de dados: 119
Autores: DUARTE, E. S.; TEIXEIRA, G. D.; FONSECA, L. M. F.; REIS, D. P.; OLIVEIRA, C. M. R.; MARRIEL, I. E.; FAGGI, A. M.
Tipo: Parte de livro
Palavras-chave: Bioindicador; Recuperação; Enzima; Enzymes.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1001580

Enzimas lignocelulolíticas da microbiota ruminal de ovinos Morada Nova e seu potencial na sacarificação do bagaço de cana-de-açúcar.
Provedor de dados: 119
Autores: RIBEIRO, R. P.
Resumo: Os ruminantes são fontes de microrganismos produtores de enzimas lignocelulolíticas, as quais podem ser utilizadas para diferentes aplicações biotecnológicas, como a degradação da fibra do bagaço de cana-de-açúcar (BCA) para uso alimentar ou para produção de bioetanol. Enzimas mais específicas são obtidas quando o próprio substrato de interesse é utilizado na produção dos microrganismos. Nesse sentido, o presente trabalho direcionou a microbiota ruminal de ovinos Morada Nova para a degradação do BCA objetivando extrair as proteínas aderidas à fibra (PAFs) e caracterizá-las quanto ao potencial de degradação do BCA in natura e pré-tratado com 10, 20, 30, 40 ou 50% de NaOH. O conteúdo ruminal foi obtido via fístula, filtrado, lavado com Solução Salina...
Tipo: Teses
Palavras-chave: Microorganismos ruminais; Pré-tratamento; Sacarificação enzimática; Hidrólise enzimática; Endoglucanase; Ruminant microorganisms; Enzymatic saccharification; Lignocellulolytic enzymes; Ovino; Enzima; Saccharum Officinarum; Sheep; Enzymes; Pretreatment; Sugarcane bagasse; Brazil.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112164

Potencial biotecnológico de actinobactérias para produção de celulase.
Provedor de dados: 119
Autores: OLIVEIRA, J. C. M. D. de; COTTA, S. P. M.; MELO, I. G.; REIS, D. P. dos; LANA, U. G. de P.; MARRIEL, I. E.
FertBio 2016.
Tipo: Parte de livro
Palavras-chave: Enzima; Streptomyces; Amycolatopsis; Enzymes.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056201

Enzymatic activity in cerrado soil under crop-livestock-forest integration system in the central region of Minas Gerais.
Provedor de dados: 119
Autores: REIS, D. P.; VIANA, M. C. M.; SANTOS, K. K. B.; MELO, I. G.; OLIVEIRA-PAIVA, C. A.; GUIMARAES, L. J. M.; OLIVEIRA, M. C.; MARRIEL, I. E.
Tipo: Parte de livro
Palavras-chave: Enzima; Nutriente; Enzymes; Soil nutrient dynamics.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078804

Characterization and phylogenetic affiliation of actinobacteria of biotechnological interest isolated from tropical soils.
Provedor de dados: 119
Autores: OLIVEIRA, J. C. M. D.; ABREU, C. S.; FIGUEIREDO, J. E. F.; LANA, U. G. P.; OLIVEIRA-PAIVA, C. A.; GOMES, E. A.; STOIANOFF, M. A. R.; MARRIEL, I. E.
Tipo: Parte de livro
Palavras-chave: Actinomiceto; Enzima; Actinobacteria; Enzymes.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078785

Protein profile in arabica coffee seeds in electrophoresis gel: importance of freeze-drying.
Provedor de dados: 119
Autores: FÁVARIS, N. A. B.; ROSA, S. D. V. F. da; FIGUEIREDO, M. A. de; COELHO, S. V. B.; VILELA, A. L. de O.; PADILHA, L.
Coffee seeds are sensitive to desiccation and are used or stored with different moisture content values, which may affect the results of quality assessment. The aim of this study was to evaluate the changes in protein profile in electrophoresis gel in coffee seeds with different moisture content values under freeze-drying and without freeze-drying. Two lots of arabica coffee seeds were used, one of newly-harvested seeds and another of stored seeds. The seeds were dried to the moisture content values of 12, 15, 20, 25, 30, 35, and 40%. The physiological quality of the seeds was assessed through the germination test, electrical conductivity, and the profiles of the enzymes SOD, CAT, PO, GOT, MDH, and EST and of heat-resistant proteins. In general, there is...
Tipo: Artigo de periódico
Palavras-chave: Coffea Arábica; Enzymes; Seeds; Freeze drying.
Ano: 2022
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150809

Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.
Provedor de dados: 119
Autores: SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F.
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase,...
Tipo: Artigo de periódico
Palavras-chave: Enzima; Clonagem; Programa de Computador; Peptídeo; Metagenomics; Enzymes; Cloning (animals); Signal peptide; Prevotella; Computer software; Screening.
Ano: 2021
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129624

Antioxidant responses of wheat plants under stress.
Provedor de dados: 119
Autores: CAVERZAN, A.; CASASSOLA, A.; BRAMMER, S. P.
Tipo: Separatas
Palavras-chave: Stress; Enzima; Trigo; Abiotic stress; Enzymes; Wheat.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067833

Laying performance and egg quality of japanese quails fed diets containing castor meal and enzyme complex.
Provedor de dados: 119
Autores: LUDKE, M. do C. M. M.; PIMENTEL, A. C. S.; LUDKE, J. V.; SILVA, J. C. N. S.; RABELLO, C. B.; SANTOS, J. S.
Título equivalente em português: Revista Brasileira de Ciência Avícola.
Tipo: Artigo de periódico
Palavras-chave: Enzyme complex; Codorna; Postura; Dieta; Suplemento Enzimático; Proteína; Laying hens; Laying performance; Egg quality; Quails; Coturnix japonica; Enzymes; Protein sources.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113611

Detecção da presença da enzima beta-galactosidase em bactérias láticas.
Provedor de dados: 119
Autores: MATOS, C. R. de; SANTOS, K. M. O. dos.
Tipo: Parte de livro
Palavras-chave: Â-galactosidase; Bactéria láctica; Intolerância à lactose; Alpha galactosidase.; Caprino; Leite de cabra; Lactose; Enzima; Goat milk; Enzymes; Food allergies; Food intolerance; Lactose intolerance.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/980366

ENZYMATIC AND ANTAGONIST ACTIVITY OF ENDOPHYTIC FUNGI FROM Sapindus saponaria L. (SAPINDACEAE)
Provedor de dados: 37
Autores: SANTOS,Caroline Menicoze dos; RIBEIRO,Amanda da Silva; GARCIA,Adriana; POLLI,Andressa Domingos; POLONIO,Julio Cesar; AZEVEDO,João Lúcio; PAMPHILE,João Alencar.
ABSTRACT Endophyte microorganisms have great biotechnological interest, with features applicable to different areas and are potentially useful in agriculture. The current study determines the biotechnological potential of endophytic fungi, isolated from leaves of Sapindus saponaria, to control phytopathogenic fungi and evaluate their enzyme production. Molecular taxonomy was performed by sequencing of the ITS1-5.8S-ITS2 ribosomal DNA region, identifying the genera Phomopsis, Sordariomycetes, Diaporthe, and Colletotrichum. In vitro antagonism against phytopathogens showed better results against Fusarium solani and provided inhibition indices between 41.8 % and 67.5 %. The endophytic strain SS81 (Diaporthe citri) presented the highest antagonism index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Endophytes; Enzymes; Microbial antagonisms; Molecular taxonomy.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200322

Fungal endophyte diversity in the leaves of the medicinal plant Myracrodruon urundeuva in a Brazilian dry tropical forest and their capacity to produce L-asparaginase
Provedor de dados: 39
Autores: Pádua,Ana Patrícia Sousa Lopes de; Freire,Karla Torres Lins de Sousa; Oliveira,Thays Gabrielle Lins de; Silva,Leticia Francisca da; Araújo-Magalhães,Gianne Rizzuto; Agamez-Montalvo,Gualberto Segundo; Silva,Iolanda Ramalho da; Bezerra,Jadson Diogo Pereira; Souza-Motta,Cristina Maria de.
ABSTRACT We studied the fungal endophyte community of the leaves of Myracrodruon urundeuva in Brazil along with their potential to produce L-asparaginase. In total, 187 endophytes were isolated. The colonization rate of leaf fragments from caatinga (10.41 %) was lower than those from brejo de altitude (39.58 %). Sequences of ITS rDNA from all endophytes revealed relationships with Ascomycota (Botryosphaeriales, Chaetothyriales, Diaporthales, Eurotiales, Glomerellales, Hypocreales, and Pleosporales) and Basidiomycota (Polyporales). The most frequent endophytes were members of the genus Diaporthe. Talaromyces was an indicator genus for caatinga while Phyllosticta, Diaporthe, and Colletotrichum were for brejo de altitude. The composition of endophytic fungi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian ecosystems; Diaporthe; Endophytes; Enzymes; Fungal richness.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100039

Accelerated ageing test and behaviour investigation of isoenzymes in sesame seeds
Provedor de dados: 1
Autores: Nery, Marcela Carlota; Rocha, Adriana de Souza; Pinho, Édila Vilela de Resende Von; Santos, Heloisa Oliveira dos; Fialho, Cintia Maria Teixeira; Nery, Fernanda Carlota.
The seeds of a sesame crop contain approximately 50% oil content, one of the main reasons for their cultivation. However, information regarding methodologies for the assessment of quality seeds of this culture are scarce. Therefore, the objective of this study was to adapt the methods of accelerated ageing tests for sesame seeds and investigate changes in the behaviour of isoenzymes. The physiological characterization of the seeds and electrophoretic analysis of the isoenzymes superoxide dismutase, esterase, catalase, alcohol dehydrogenase, malate dehydrogenase and isocitrate lyase were also performed. For the accelerated ageing test, the seeds were subjected to the traditional method, with a saturated NaCl solution, at ageing times of 0, 24, 48, 72, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Oilseed; Vigour; Quality; Sesamum indicum.; Produção e tecnologia de sementes.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39449

Utilização de complexo enzimático em rações para codornas de corte - doi: 10.4025/actascianimsci.v33i3.11216
Provedor de dados: 2
Autores: Iwahashi, Alexandre Shigueki; Universidade Estadual de Maringá; Furlan, Antônio Claudio; Universidade Estadual de Maringá; Scherer, Carina; Universidade Estadual de Maringá; Ton, Ana Paula Silva; UEM; Lorençon, Leticia; UEM; Scapinello, Cláudio; Universidade Estadual de Maringá.
Foram conduzidos dois experimentos com o objetivo de avaliar os efeitos da suplementação enzimática das rações à base de milho e farelo de soja sobre o desempenho e metabolizabilidade dos nutrientes em codornas de corte. Os tratamentos foram: controle positivo para atendimento das exigências; controle positivo suplementado com complexo enzimático e controles negativos suplementados reduzidos em 2 e 4% nos teores de energia metabolizável e aminoácidos. Na fase inicial, observou-se aumento no consumo de ração (p &lt; 0,05) no tratamento controle negativo reduzido em 4% na energia metabolizável e aminoácidos em comparação aos tratamentos controle positivos. Na fase de crescimento o consumo de ração, conversão alimentar e rendimento de carcaça não diferiram (p...
Tipo: Pesquisa de campo ; análise laboratorial
Palavras-chave: 5.04.03.02-8 balanço de nutrientes; Carboidrases; Coturnix coturnix sp.; Desempenho; Enzimas; Rendimento de carcaça nutrient balance; Carbohydrases; Coturnix coturnix sp.; Performance; Enzymes; Carcass yield.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/11216

Associação de carboidrase e fitase em dietas valorizadas para poedeiras semipesadas - doi: 10.4025/actascianimsci.v34i3.12735
Provedor de dados: 2
Autores: Silva, Leandro Moreira; Instituto Federal Minas Gerais - Campus Bambuí; Geraldo, Adriano; Instituto Federal Minas Gerais - Campus Bambuí; Vieira Filho, Javer Alves; Instituto Federal Minas Gerais - Campus Bambuí; Machado, Luiz Carlos; Instituto Federal Minas Gerais - Campus Bambuí; Brito, Jerônimo Ávito Gonçalves de; Universidade Federal do Recôncavo Baiano; Bertechini, Antonio Gilberto; Universidade Fderal de Lavras.
O objetivo do estudo foi avaliar o efeito da associação de carboidrases e fitase em dietas de poedeiras. Foram utilizadas 300 poedeiras Isa Brown com 26 semanas, submetidas aos tratamentos: Controle Positivo: sem enzimas e valorização dos nutrientes; T2: valorização em 1,5 e 6% a EMA (kcal kg-1), do milho e o farelo de soja, respectivamente, e redução nos níveis nutricionais conforme a matriz nutricional para a fitase; T3: valorização da EMA do milho e o farelo de soja (idem T2) e redução nos níveis de cálcio e fósforo conforme matriz nutricional da fitase; T4: T2 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase; T5: T3 suplementado com 100 g t-1 de carboidrases e 30 g t-1 de fitase. Foi utilizado delineamento inteiramente casualizado, seis...
Tipo: Pesquisa de campo
Palavras-chave: 5.04.03.00-1 desempenho; Qualidade de ovos; Enzimas  energia metabolizável; Fósforo disponível; Valorização de dietas performance; Egg quality; Enzymes; Metabolizable energy; Disponible phosphorus; Valorization of diets.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12735

Effect of protease supplementation on production performance of laying hens
Provedor de dados: 2
Autores: Vieira Filho, Javer Alves; Geraldo, Adriano; Machado, Luiz Carlos; Brito, Jerônimo Ávito Golçalvez de; Bertechini, Antônio Gilberto; Murakami, Elise Saori Floriano.
The experiment was conducted with the objective of evaluating the effect of protease enzyme supplementation on performance parameters and quality shell of laying hens. We used 240 Isa Brown commercial laying hens with 44 weeks of age. A completely randomized split-plot design (5 periods of 21 days) with four treatments and six replications (10 hens per replication) was used. The experimental diets were formulated according to the requirements of the breed. The following parameters were evaluated: egg production, feed intake, feed conversion, average egg weight, egg loss, specific gravity, percentage and shell thickness. After collection, the data were analyzed with SISVAR Statistical Package, and the means compared by SNK test at 5% probability. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Laying hen farming; Enzymes; Grains; Nutrition.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22830

Evaluation of the protective effect of guava fruits and leaves on oxidative stress   - doi: 10.4025/actascibiolsci.v36i1.19839
Provedor de dados: 3
Autores: Freire, Juliana Mesquita; Faculdades Unidas do Vale do Araguaia; Abreu, Celeste Maria Patto de; Universidade Federal de Lavras; Duarte, Stella Maris da Silveira; Universidade Federal de Alfenas; Paula, Fernanda Borges Araújo de; Universidade Federal de Alfenas; Lima, Adriene Ribeiro; Universidade Federal de Lavras.
Oxidative stress is an imbalance between reactive oxygen species and antioxidant capacity of action. Thus, research, alternative to mitigate the damaging effects of oxidative stress, improving the body´s antioxidant capacity, prevented the disease and its complicatons. The leaves and fruits of guava are rich in the antioxidants. This work aimed to study the effect of flour and ethanolic/acetone extracts leaves and fruits of guava on lipid oxidation in rats with hypercholesterolemic. The flour and extracts decreased leves of triglycerides and non – HDL cholesterol and increased in the HDL. Cholesterol levels decreased only leaves and fruits. The activity of GPx decreased in samples serum in all treatments  and the SOD only the extracts were effective. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioquímica antioxidants; Enzymes; Malonic dialdehyde Metabolismo e bioenergéetica.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/19839

Annual production of grass silage for biogas: effects of fibrolytic enzyme additives on ensilage efficiency and specific methane yields
Provedor de dados: 111
Autores: Nolan, Pearl; Doyle, Evelyn; O'Kiley, Padraig.
The aims of this study were to quantify the effects of fibrolytic enzyme additives applied to each of four consecutive cuts of unwilted grass at ensiling on ensilage characteristics and specific CH4 yields (SMY) per unit mass and per unit land area. In light of the importance of the primary growth yield, the effects of the timing of Cut 1 were also investigated. Furthermore, the mass-SMY and area-SMY effect of any effluent produced during ensilage was determined.At each of four cuts that comprised annual growth, samples from four replicate plots of Lolium perenne and of Phleum pratense were untreated (control) or were treated with either of two fibrolytic enzymes (ENZ 1 and ENZ 2) prior to ensiling for 120 days. The mass-SMY of silages and effluents were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grass yield; Silage; Fibre; Enzymes; Effluent; CH4 yield per unit land area.
Ano: 2018
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4159

Metabolic responses of channel catfish (Ictalurus punctatus) exposed to phenol and post-exposure recovery
Provedor de dados: 42
Autores: Moraes,Fernanda D.; Rossi,Priscila A.; Figueiredo,Juliana S.L.; Venturini,Francine P.; Cortella,Lucas R.X.; Moraes,Gilberto.
Metabolic adjustments were studied in channel catfish Ictalurus punctatus exposed to 1.5 mg L-1 of phe nol (10% LC50) for four days and recovered for seven days. Lower triacylglycerol (TGA) stores and increased muscle fat free acids (FFA) suggest fat catabolism in muscle. Remarkable liver FFA decrease (-31%) suggests liver fat catabolism as well. Increased muscular ammonia levels and ASAT (aspartate aminotransferase) and decreased plasma aminoacids suggest higher muscular amino acid uptake. Constant levels of glucose and increased liver glycogen stores, associated with lower amino acids in plasma, indicate gluconeogenesis from amino acids. This is supported by higher hepatic ALAT and ASAT. Higher hepatic LDH followed by lower plasma lactate may indicate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic adjustments; Enzymes; Lipid stores; Intermediary metabolism; Recovery; Xenobiotic.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000300865

Ecology and biotechnological potential of bacterial community from three marine sponges of the coast of Rio de Janeiro, Brazil
Provedor de dados: 42
Autores: ARAÚJO,FÁBIO V. DE; NETTO,MARCELLE C.M.; AZEVEDO,GUSTAVO P.; JAYME,MARCELLY M.A.; NUNES-CARVALHO,MONICA C.; SILVA,MARIANA M.; CARMO,FLÁVIA L. DO.
ABSTRACT Marine sponges has been a large reservoir of microbial diversity, with the presence of many species specific populations as well as producing biologically active compounds, which has attracted great biotechnological interest. In order to verify the influence of the environment in the composition of the bacterial community present in marine sponges and biotechnological potential of bacteria isolated from these organisms, three species of sponges and the waters surrounding them were collected in different beaches of Rio de Janeiro, Brazil. The profile of the bacterial community present in sponges and water was obtained by PCR-DGGE technique and the biotechnological potential of the strains isolated by producing amylase, cellulase, protease and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial diversity; Biosurfactants; Enzymes; Marine sponges; PCR-DGGE.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602785

Enzymatic reactions involving the heteroatoms from organic substrates
Provedor de dados: 42
Autores: MARQUES NETTO,CATERINA G.C.; PALMEIRA,DAYVSON J.; BRONDANI,PATRÍCIA B.; ANDRADE,LEANDRO H.
ABSTRACT Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These reports include mainly native and mutated enzymes and whole cells biocatalysis. Herein, we describe the historical background along with the progress of biocatalyzed reactions involving the heteroatom(S, Se, B, P and Si) from hetero-organic substrates.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Heteroatom; Biocatalysis; Enzymes; Biotransformation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200943

Microbiological Indicators of Soil Quality Under Native Forests are Influenced by Topographic Factors
Provedor de dados: 42
Autores: TEIXEIRA,ANITA F.S.; SILVA,JACQUELINE S.; VILELA,LAÍZE A.F.; COSTA,PATRÍCIA F.; COSTA,ELAINE M. DA; GUIMARÃES,AMANDA A.; SANTOS,JESSÉ V. DOS; SILVA,SÉRGIO H.G.; CARNEIRO,MARCO AURÉLIO C.; MOREIRA,FATIMA M.S.
Abstract: Several microbiological indicators of soil quality present high sensitivity, but little is known about the influence of topographic factors on them. This work aimed to evaluate variability of biological indicators of soil quality across a hillslope under native forest and the influence of topographic factors on them. Four positions on a hillslope were evaluated. Activity of the enzymes β-glucosidase, acid phosphatase, urease and fluorescein diacetate (FDA) hydrolysis were determined, as well as basal and substrate-induced respiration, and density of microorganisms: total bacteria, total fungi, actinobacteria, phosphate solubilizers, ammonifiers, native rhizobia, free-living N2-fixing bacteria, spores of arbuscular mycorrhizal fungi and percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological indicators; Digital elevation model; Enzymes; Microbial activity; Random forest.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700866

Enzymes immobilized in Langmuir-Blodgett films: Why determining the surface properties in Langmuir monolayer is important?
Provedor de dados: 42
Autores: CASELI,LUCIANO.
ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Langmuir-Blodgett; Enzymes; Biosensors; Air-water interface.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200631

Antimicrobial and enzymatic activity of anemophilous fungi of a public university in Brazil
Provedor de dados: 42
Autores: SOBRAL,LAUREANA V.; MELO,KELLY N.; SOUZA,CLECIANA M.; SILVA,SÍLVIO F.; SILVA,GILVANIA L.R.; SILVA,ANDRESSA L.F.; WANDERLEY,KATHARINE A.A.; OLIVEIRA,IDJANE S.; CRUZ,ROBERTA.
ABSTRACT To the fungal microbiota the UFPE and biotechnological potential enzymatic and antimicrobial production. Air conditioned environments were sampled using a passive sedimentation technique, the air I ratio and the presence of aflatoxigenic strains evaluated for ANVISA. Icelles were to determine the enzymatic activity of lipase, amylase and protease metabolic liquids to determine antimicrobial activity. Diversity was observed in all CAV environments, CFU/m3 ranged from 14 to 290 and I/E ratio from 0.1 to 1.5. The of the fungal genera were: Aspergillus (50%), Penicillium (21%), Talaromyces (14%), Curvularia and Paecilomyces (7% each). Aspergillus sydowii (Bainier &amp; Sartory) Thom &amp; Church presented enzymatic activity and the Talaromyces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Air quality; Bacteria; Enzymes; Filamentous fungi; Refrigerated environments.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502327

Effect of herbicides in the oxidative stress in crop winter species
Provedor de dados: 42
Autores: ALVES,CARLA; COSTA,EDUARDA; SOFIATTI,JESSICA R.; FORTE,CESAR T.; WINTER,FÁBIO L.; HOLZ,CINTHIA M.; KAIZER,ROSILENE R.; GALON,LEANDRO.
ABSTRACT Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Enzymes; Agricultural plants; PROTOX system.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401533

Alterations in muscular enzymes of horses competing long-distance endurance rides under tropical climate
Provedor de dados: 45
Autores: Teixeira-Neto,A.R.; Ferraz,G. C.; Moscardini,A. R. C.; Balsamão,G. M.; Souza,J. C. F.; Queiroz-Neto,A.
This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Endurance; Enzymes; Muscle.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300004

Performance and morphology of intestinal mucosa of broilers fed mannan-oligosaccharides and enzymes
Provedor de dados: 45
Autores: Oliveira,M.C.; Rodrigues,E.A.; Marques,R.H.; Gravena,R.A.; Guandolini,G.C.; Moraes,V.M.B.
The performance and the morphology of intestinal mucosa of broilers fed mannan-oligosaccharides (MOS) and enzymes (E) from one to 21-day-old were evaluated using 750 one-day-old chicks, assigned to a 2 x 2 + 1 factorial design - two levels of MOS (0 and 0.1%), two levels of E (0 and 0.05%) plus an antibiotic positive control diet - performing five treatments of five replications each one. MOS x E interaction was significant for both duodenal (P&lt;0.002 and P&lt;0.002) and ileal (P&lt;0.04 and P&lt;0.05) perimeters and heights of villi, being the values lower in the mucosa of birds fed non-supplemented diets. MOS based-diet determined an increase on perimeter of jejunal villi (P&lt;0.05). Compared with antibiotic treatment group, villi perimeter...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Nutrition; Enzymes; Prebiotic.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200025

Glycolysis and gluconeogenesis in the liver of catfish fed with different concentrations of proteins, lipids and carbohydrates
Provedor de dados: 45
Autores: Melo,J.F.B.; Lundstedt,L. M; Inoue,L.A.K.; Metón,I.; Baanante,I.V.; Moraes,G.
ABSTRACT The activities of enzymes from a number of metabolic pathways have been used as a tool to evaluate the best use of nutrients on fish performance. In the present study the catfish Rhamdia quelen was fed with diets containing crude protein-lipid-carbohydrate (%) as follows: treatment (T) T1: 19-19-44; T2: 26-15-39; T3: 33-12-33; and T4: 40-10-24. The fish were held in tanks of re-circulated, filtered water with controlled temperature and aeration in 2000L experimental units. The feeding experiment lasted 30 days. The following enzymes of the carbohydrate metabolism were determined: Glucokinase (GK), Phosphofructokinase 1 (PFK-1), Pyruvate kinase (PK), Fructose-1,6-biphosphatase 1 (FBP-1). The activities of 6 phosphogluconate dehydrogenase (6PGDH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolism; Enzymes; Nutrition; Adaptation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501251

Malassezia pachydermatis: enzymes production in isolates from external ear canal of dogs with and without otitis
Provedor de dados: 45
Autores: Coutinho,S.D.A.
The relationship between production of enzymes and pathogenicity of Malassezia pachydermatis strains was studied. Thirty strains of M. pachydermatis isolated from dogs were used: 15 originating from animals with external otitis and 15 obtained from ears of asymptomatic dogs. The samples, obtained by introduction of a sterile swab into the auditory canal after cleaning the auricle with alcohol-ether solution, were seeded onto Sabouraud dextrose agar with chloramphenicol (100mg/l) and were incubated at 37°C for up to two weeks. The colonies were identified according to their macro-and-micromorphology. All strains were investigated for the production of chondroitin-sulphatase, hyaluronidase, phospholipase and proteinase. Most of the strains showed a high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Malassezia pachydermatis; Enzymes; Otitis; Microbiota.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000800003

Temperature-dependent alterations in metabolic enzymes and proteins of three ecophysiologically different species of earthworms
Provedor de dados: 52
Autores: Tripathi,G; Kachhwaha,N.; Dabi,I.; Bandooni,N.
The effects of varying temperatures (12 - 44° C) on the specific activity of cytoplasmic malate dehydrogenase ((cMDH), mitochondrial malate dehydrogenase (mMDH) and lactate dehydrogenase (LDH) of some earthworms (Metaphire posthuma, Perionyx sansibaricus and Lampito mauritii) were studied. The effects of different temperatures on supernatant and mitochondrial protein contents were also investigated. The specific activities of cMDH, mMDH and LDH of the earthworms decreased gradually as a function of increasing temperature from 12 to 44°C. Higher metabolic energy was needed to maintain the activity at low temperatures. Hence, the earthworms showed increased enzyme specific activity at low temperatures. However, the protein content increased upto 28°C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Proteins; Earthworms; Temperatures.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400017

Enzymatic programs for broilers
Provedor de dados: 52
Autores: Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda.
Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P&lt;0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P&lt;0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030

Isolation and Identification of a Potential Amylolytic Probiotic Bacterium from the Gut of Jundiá Catfish, Rhamdia quelen.
Provedor de dados: 52
Autores: Poletto,Tatiana Vieira; Vieira,Cleide Rosana Werneck; Silva,Carlos Peres; Fracalossi,Debora Machado.
ABSTRACT This study aimed to isolate a potential probiotic amylolytic strain from the gut of jundiá catfish to improve carbohydrate digestibility in fish. Two of 31 strains isolated from the foregut of Rhamdia quelen were able to grow on starch-agar medium and were considered amylolytic. The strain that presented higher amylolytic potential, based on a qualitative amylase assay, was chosen. The strain was phenotypically characterized and analysed to determine bile and pH tolerance and extracellular quantitative amylase activity. The probiotic candidate, identified as Aeromonas veronii, showed the ability to survive stresses from a range of pH and bile salt conditions and secreted an interesting enzymatic profile, which may exhibit a synergistic effect when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Probiotics; Aquaculture; Intestinal microbiology; Aeromonas veronii.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100323

Influence of stationary and bioreactor cultivation on Lentinula edodes (berk) pegler lignocellulolitic activity
Provedor de dados: 52
Autores: Regina,Magali; Broetto,Fernando; Giovannozzi-Sermanni,Giovanni; Marabotini,Rosita; Peranni,Claudio.
This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25º C and in a bioreactor with four complete rotations daily at 25º C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shiitake; Enzymes; Agricultural residues.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200001

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems
Provedor de dados: 52
Autores: Yan,Xu; Souza,Maria Aparecida; Pontes,Marcela Z. R.; Vitolo,Michele; Pessoa Júnior,Adalberto.
From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400030

Isolation of lipase producing fungi from palm oil Mill effluent (POME) dump sites at Nsukka
Provedor de dados: 52
Autores: Nwuche,Charles Ogugua; Ogbonna,James Chukwuma.
In this study, twelve fungal lipase producing strains belonging to Aspergillus, Penicillium, Trichoderma and Mucor genera were isolated from palm oil mill effluent composts. The Aspergillus spp. were more frequent (42%) and was present in all the samples assayed. Mucor sp. was the least encountered (8.3%).The lipase producing profile showed that Trichoderma (8.07-8.24 u/mL) and Aspergillus (6.25 -7.54 u/mL) spp. were the highest lipase producers while Mucor (5.72 u/mL) was the least.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Lipases; Triglycerides; Esterification; Waste water treatment.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100015

Enzymatic Activity in the Gastrointestinal Tract of Pimelodus maculatus (Teleostei, Siluriformes) in Two Neotropical Reservoirs with Different Trophic Conditions
Provedor de dados: 52
Autores: Duarte,Silvana; Bemquerer,Marcelo; Araújo,Francisco Gerson.
Enzymatic activities for digestion of proteins and carbohydrates were compared among three organs of the digestive system of Pimelodus maculatus in two reservoirs with different trophic conditions during the winter of 2006. The aim was to test the hypothesis that enzymatic activity for the digestion of proteins and carbohydrates differed among organs and that such activities differ between the trophic state of the environment. Enzymatic activities were determined through the assays of specificity for trypsin, chymotrypsin and β-glucosidase enzymes. The intestine had higher trypsin-like enzymatic activities compared to the stomach and liver. The highest β-glucosidase activity was found in the liver compared to the stomach and intestine in the oligotrophic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Catfish; Physiology; Freshwater fishes; Digestive tract.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400605

Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo)
Provedor de dados: 52
Autores: Carrim,Aysha Jussara Ivonilde; Barbosa,Edweis Cândida; Vieira,José Daniel Gonçalves.
The objectives of this work was to isolate endophytic bacteria from Jacaranda decurrens Cham. and screening of some enzymes of biotechnological interest. Ten (10) bacterial species were isolated and identified from the leaves and steams. All the isolates presented enzymatic activity, which was ranked as follows: proteolytic (60%) and amilolytic activity (60%), lipolytic (40%), esterasic (40%). However, cellulolytic and pectinolytic activities were not detected. This is the first report on the isolation and identification of endophytic bacteria from Jacaranda decurrens Cham.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic bacteria; Jacaranda decurrens; Enzymes.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400001

Two related thrombin-like enzymes present in Bothrops atrox venom
Provedor de dados: 56
Autores: Petretski,J.H.; Kanashiro,M.; Silva,C.P.; Alves,E.W.; Kipnis,T.L.
This article describes the presence of two new forms of a thrombin-like enzyme, both with apparent molecular masses of 38 kDa, in Bothrops atrox venom. Both share the ability to cleave fibrinogen into fibrin and to digest casein. Both present identical Km on the substrate BApNA. Their N-terminal amino acid sequences are identical for 26 residues, sharing 80% homology with batroxobin and flavoxobin. Two groups of monoclonal antibodies (mAbs) raised against the purified enzyme forms recognized different epitopes of the putative corresponding enzymes present in B. atrox crude venom. On Western blotting analysis of B. atrox crude venom, mAbs 5DB2C8, 5AA10 and 5CF11, but not mAbs 6CC5 and 6AD2-G5, revealed two or more protein bands ranging from 25 to 38 kDa. By...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venoms; Bothrops atrox; Enzymes; Thrombin-like enzymes; Blood coagulation system.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100005

Cellulase and xylanase activity during the decomposition of three aquatic macrophytes in a tropical oxbow lagoon
Provedor de dados: 58
Autores: Sciessere,L; Cunha-Santino,M. B; Bianchini Jr,I.
Due to the connection between enzymatic activity and degradation of different fractions of organic matter, enzyme assays can be used to estimate degradation rates of particulate and dissolved organic carbon in freshwater systems. The aim of this study was to quantify and model the enzymatic degradation involving the decomposition of macrophytes, describing temporal activity of cellulases (EC 3.2.1.4 and EC 3.2.1.91) and xylanase (EC 3.2.1.8) during in situ decomposition of three aquatic macrophytes (Salvinia sp., Eichhornia azurea and Cyperus giganteus) on the surface and water-sediment interface (w-s interface) of an oxbow lagoon (Óleo lagoon) within a natural Brazilian Savanna Reserve. Overall, the enzymatic degradation of aquatic macrophytes in Óleo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Litterbags; Mass decay; Particulate organic matter.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300009

Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material
Provedor de dados: 58
Autores: Cardoso,Wilton Soares; Queiroz,Paula Viana; Tavares,Gabriella Peterlini; Santos,Fernando Almeida; Soares,Filippe Elias de Freitas; Kasuya,Maria Catarina Megumi; Queiroz,José Humberto de.
ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enzymes; Cellulases; Fungus; Hydrolysis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400879

Screening of actinomycetes from earthworm castings for their antimicrobial activity and industrial enzymes
Provedor de dados: 58
Autores: Kumar,Vijay; Bharti,Alpana; Negi,Yogesh Kumar; Gusain,Omprakash; Pandey,Piyush; Bisht,Gajraj Singh.
Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%), Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Enzymes; Earthworm castings; Actinomycetes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100022

Chemical products induce resistance to Xanthomonas perforans in tomato
Provedor de dados: 58
Autores: Itako,Adriana Terumi; Tolentino Júnior,João Batista; Silva Júnior,Tadeu Antônio Fernandes da; Soman,José Marcelo; Maringoni,Antonio Carlos.
The bacterial spot of tomato, caused by Xanthomonas spp., is a very important disease, especially in the hot and humid periods of the year. The chemical control of the disease has not been very effective for a number of reasons. This study aimed to evaluate, under greenhouse conditions, the efficacy of leaf-spraying chemicals (acibenzolar-S-methyl (ASM) (0.025 g.L−1), fluazinam (0.25 g.L−1), pyraclostrobin (0.08 g.L−1), pyraclostrobin + methiran (0.02 g.L−1 + 2.2 g.L−1), copper oxychloride (1.50 g.L−1), mancozeb + copper oxychloride (0.88 g.L−1 + 0.60 g.L−1), and oxytetracycline (0.40 g.L−1)) on control of bacterial spot. Tomatoes Santa Clara and Gisele cultivars were pulverized 3 days before inoculation with Xanthomonas perforans. The production of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum lycopersicum; Bacterial spot; Control; Enzymes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300701

Electrochemical biosensors in pharmaceutical analysis
Provedor de dados: 60
Autores: Gil,Eric de Souza; Melo,Giselle Rodrigues de.
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002

Activation of nitrate reductase of cashew leaf by exogenous nitrite
Provedor de dados: 61
Autores: Viégas,Ricardo Almeida; Silveira,Joaquim Albenísio Gomes.
Nitrate reductase (NR) plays a central role in plant primary metabolism and exhibits complex regulation mechanisms for its catalytic activity. There is controversy in the literature concerning the possible direct effect of NO2- on the stimulation and/or activation of NR activity. The influence of NO2- was studied on the NR activity in the leaves of 30-day-old cashew (Anacardium occidentale L.) seedlings. Addition of NO2- to the reaction mixture containing leaf enzymatic extract resulted in a remarkable increase in NR activity. A trace amount (5 mumol.L-1) of NO2- was required to achieve full NR activity. The in vitro NR-activity showed a steady time-dependent increase when incubated in the presence of only NO3- + NO2-. In contrast, in vitro NR activity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale; Enzymes; NADH; Nitrate metabolism; Nitrate reductase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100005

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.
Provedor de dados: 61
Autores: Shweta,Mishra; Agrawal,S.B.
The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg-1 soil) and Ni (68 µmol.kg-1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinaicia oleracea; Biomass; Cadmium; Enzymes; Nickel; Pigments; Ultraviolet-B.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200007

Effects of hydric deficiency on gas exchange parameters and metabolism of Eucalyptus grandis clones
Provedor de dados: 61
Autores: Coscolin,Renata B. S.; Broetto,Fernando; Marchese,José A.; Campohermoso,Marco C.; Paladini,Marcos V.
The metabolic effects caused by hydric deficiency (HD) on Eucalyptus grandis clones were assessed by an experiment where plants were cultivated in four blocks. The first was the control block, normally irrigated, whereas the other three blocks were submitted to cycles of hydric deficiency. Analysis of photosynthetic efficiency, enzymatic activity of antioxidant response system, level of pigments and L-proline concentration were performed to evaluate the HD effects. Results showed that HD altered some parameters related to photosynthetic activity, pigments accumulation, proline and enzymatic activity. Clone 433 of E. grandis presented higher response ability to HD.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Eucalypt; Hydric deficiency; Photosynthesis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000400002

The influence of processing and long-term storage on the antioxidant metabolism of acerola (Malpighia emarginata) purée
Provedor de dados: 61
Autores: Oliveira,Luciana S.; Rufino,Maria S. M.; Moura,Carlos F. H.; Cavalcanti,Fabio R.; Alves,Ricardo E.; Miranda,Maria R. A.
This study evaluated the effect of the processing and long-term storage on the antioxidant potential and activity of antioxidant enzymes of frozen purées from six acerola clones. Ripe acerolas from clones BRS 235, BRS 236, BRS 237, BRS 238, II47/1 and BRS 152 were harvested; the pulp was processed, packed in sealed polyethylene plastic bags and stored in a domestic freezer at -18°C for 11 months. Samples of each clone were analyzed on harvest/processing day and every 30 days after for bioactive antioxidant compounds, antioxidant enzyme activity and total antioxidant activity. Acerola purées presented a decrease of non-enzyme antioxidants and an increase of antioxidant enzymes activities, indicatives of a compensatory mechanism between enzymatic and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulp; Enzymes; Vitamin C; Postharvest; Frozen.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200007

Antioxidant system response induced by aluminum in two rice cultivars
Provedor de dados: 61
Autores: Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da.
The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004

Use of Enzymatic Biosensors as Quality Indices: A Synopsis of Present and Future Trends in The Food Industry
Provedor de dados: 117
Autores: Serna Cock,Liliana; Zetty Arenas,Ana María; Ayala Aponte,Alfredo.
Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities....
Tipo: Journal article
Palavras-chave: Biosensors; Enzymes; Analysis in food; Safety; Quality; Control processes.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200017

Enzyme activity in soybean seeds produced under foliar application of manganese
Provedor de dados: 64
Autores: Carvalho,Everson Reis; Oliveira,João Almir; Von Pinho,Édila Vilela de Resende; Costa Neto,Jaime.
Several factors affect the quality of soybean seeds, including the mineral nutrition of plants. Manganese (Mn) is an important nutrient because it has as one of its functions the enzyme activation. The aim of this study was to evaluate enzyme activity in seeds of soybean cultivars produced with foliar application of different doses of Mn. The experiment was conducted at the Universidade Federal de Lavras (UFLA) (Federal University of Lavras) in randomized blocks with three replications and 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and its genetically modified RR derived (BRS Celeste and BRS Baliza RR; BRSGO Jataí and BRS Silvânia RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha-1) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymes; Glycine max; Quality; Micronutrients.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400001

Physicochemical and enzimatic transformations of "eva" apples stored under modified atmosphere
Provedor de dados: 64
Autores: Thomas,Ariela Betsy; Pires,Caroline Roberta Freitas; Lima,Luiz Carlos de Oliveira.
The need for a permanent market supply of fresh fruit has generated great interest in postharvest techniques that can promote useful life and quality of the fruit. The aim of this study was to evaluate the efficiency of atmospheric modification using different polymeric films in maintaining postharvest quality and prolonging the shelf life of Eva (Mallus sp) apples. For this, fruits from the city of Barbacena - MG, selected and sanitized were packed in plastic trays and wrapped in the corresponding films. The films used were: polypropylene - 20 µm, low-density polyethylene - 14 µm and high-density polyethylene - 7 µm. Another group of fruits (control) was not submitted to a modified atmosphere. Subsequently, all the the packages were stored under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mallus sp; Packaging; Firmness; Enzymes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000400008

Quality of fresh-cut avocado (Persea americana Mill.) stored under different temperatures
Provedor de dados: 64
Autores: Pinheiro,Ana Carla Marques; Vilas Boas,Eduardo Valério de Barros; Silva,Lucas Carvalho e; Alves,Alessandra de Paiva; La Selva,Marcelo; Chitarra,Adimilson Bosco.
The goal of this work was to evaluate the effect of different storage temperatures on the quality maintenance of fresh-cut 'Fortuna' avocado (Persea americana Mill.). The fruit was selected, washed and sanitized with sodium hypochlorite solution (190 ppm total residual chlorinee) for 15 minutes. After that, the tip was cut and the fruit was also cut in halves, peeled and the pit removed, manually. Then the halves were immersed in 0.5% calcium chlorite + 0.5% cysteine solution for 2 minutes, to avoid darkening and packed in rigid plastic covered with 20 micrometer thick plastic wrap (PVC film) with a permeability to O2, CO2 and water vapor of 15,000, 80,000 and 390 cm³/m²/day, respectively. The product was stored under 3 different temperatures 0, 5, 10ºC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Appearance; Acceptability; Enzymes; Firmness; Avocado; Persea Americana.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000400021

Enzyme activity and biochemical changes during production of Lentinula edodes (Berk.) Pegler
Provedor de dados: 116
Autores: SOUSA,Maiara Andrade de Carvalho; COSTA,Lívia Martinez Abreu Soares; PEREIRA,Thiago Souza; ZIED,Diego Cunha; RINKER,Danny Lee; DIAS,Eustáquio Souza.
Abstract  Shiitake is an important edible and medicinal mushroom cultivated worldwide. Its cultivation involves a complex process of browning that precedes the primordia initiation. The present work through the evaluation of enzymes, lectin and β-glucan during the cultivation cycle of Lentinula edodes, the degradation of cellulose, hemicellulose and lignin, attempted to correlate these with mushroom yield. Strains UFLA-LE1, UFLA-LE2 and UFLA-LE6 consumed significantly more hemicelluloses than the other three strains with strain LE5 consuming significantly the most lignin of all six strains. Strains UFLA-LE4 and UFLA-LE6 were significantly the most productive. The laccase activity increased continuously until the end of the cultivation for all strains....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shiitake; Lectin; Enzymes; Β-glucan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300774

Influence of carbon source, pH, and temperature on the polygalacturonase activity of Kluyveromyces marxianus CCMB 322
Provedor de dados: 116
Autores: Oliveira,Rodrigo de Queiroz; Valasques Junior,Gildomar Lima; Uetanabaro,Ana Paula Trovatti; Koblitz,Maria Gabriela Bello; Góes-Neto,Aristóteles; Rosa,Carlos Augusto; Assis,Sandra Aparecida de.
Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. Two kinds of yeast can be discerned regarding the production of enzymes. One group includes those which can produce enzymes in the absence of an inducer, and the other group comprises the yeasts that produce enzymes in the presence of an inducer. The objective of this study was to investigate the influence of pectic substances, glucose, pH, and temperature on the polygalacturonase activity by Kluyveromyces marxianus CCMB 322. The yeast was grown in a fermentation broth containing different concentrations of glucose and pectic substances. The polygalacturonase activity was determined by the DNS method, and the pH and temperature were optimized...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeasts; Enzymes; Thermostability; Pectic substances; Biotechnology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300012

Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp
Provedor de dados: 116
Autores: Pagnussatt,Fernanda Arnhold; Kupski,Larine; Darley,Francine Toralles; Filoda,Paula Freitas; Ponte,Émerson Medeiros Del; Garda-Buffon,Jaqueline; Badiale-Furlong,Eliana.
The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms' primary metabolism. The results indicate that phenolic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Natural antifungal agent; Structural compounds; Enzymes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000500012

Oxidative enzymes activity in sugarcane juice as a function of the planting system
Provedor de dados: 116
Autores: Marques,Tadeu Alcides; Rampazo,Érick Malheiros; Marques,Patricia Angélica Alves.
In Brazil, the largest producer of sugarcane in the world, the industrial process transforms this crop into ethanol and/or granulated sugar. Some cultivars exhibit enzymatic browning in the extracted sugarcane juice at levels harmful to the manufacturing process of white granulated sugar. The objective of this study was to assess the effect of sugarcane straw used as soil coverage, the use of different planting systems, and treatments with hydrogel polymer on enzymatic activity. The cultivar RB 86 7515 was sampled for 8 months; the first sample was obtained by cutting the upper portion of the stalk at the internode, which was taken to the laboratory for determination of the enzymatic activity of polyphenoloxidase (PPO) and peroxidase (POD). The soil...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saccharum; Straw coverage; Enzymes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100021

Studies of chemical and enzymatic characteristics of Yacon (Smallanthus sonchifolius) and its flours
Provedor de dados: 116
Autores: Pereira,Juciane de Abreu Ribeiro; Barcelos,Maria de Fátima Píccolo; Pereira,Michel Cardoso De Angelis; Ferreira,Eric Batista.
Due to the importance of studies on yacon related to health, its in natura pulp, in natura peel, pulp flour, and peel flour were chemically analyzed in terms of its centesimal composition, specific minerals, total dietary fiber and fractions, pH, total soluble solids, total titratable acidity, tannins, oxalic acid, and nitrate. The polyphenoloxidase and peroxidase enzymatic activities were evaluated for in natura pulp and peel only. Yacon pulp and peel flour presented average yield of 7.94% and 10.86%, respectively. The in natura pulp presented a higher moisture and carboydrate content and lower lipid, protein, total dietary fiber, and ash than those of the peel flour. The same pattern was observed for pulp flour when compared to peel flour. The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dietary fiber; Antinutritional factors; Enzymes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100012

Polyphenoloxidase and peroxidase in avocado pulp (Persea americana Mill.)
Provedor de dados: 116
Autores: Vanini,Lucimara Salvat; Kwiatkowski,Angela; Clemente,Edmar.
The aim of the present investigation was to evaluate the enzymatic activity of polyphenoloxidase and peroxidase in avocado pulps, from the Northwest area of Paraná-Brazil, in order to compare the varieties on their enzymatic activity for both, minimum and industrial processing. Enzymatic extracts were prepared from avocado pulp of Choquete, Fortuna and Quintal varieties, in green and ripe maturation stage. Thermal treatment was applied with temperatures 60, 65, 70, 75 and 80 °C. The enzymatic activities were determined by using spectrophotometer. A decline of polyphenoloxidase activity was observed in all of the varieties when both, temperature and time increased. Total inactivation of enzymes was not observed in the largest temperature. Fortuna and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persea americana Mill; Enzymes; Temperature; Maturation stage.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200036

Pseudomonas spp. and P. fluorescens: population in refrigerated raw milk
Provedor de dados: 65
Autores: Almeida,Kelly Molin de; Bruzaroski,Samera Rafaela; Zanol,Daniel; Melo,Marcela de; Santos,Joice Sifuentes dos; Alegro,Lina Casale Aragon; Botaro,Bruno Garcia; Santana,Elsa Helena Walter de.
ABSTRACT: Raw milk samples were collected from cooling tanks (after they cooled for 48 h) in five dairy farms and the corresponding bulk tank (bulk milk transportation, BMT) when they arrived to the industry. Routine physical chemical analyzes and quantification of psychrotrophic ( Pseudomonas spp. and P. fluorescens ) and aerobic mesophilic (AM) populations were performed. Only relative density and titratable acidity values for samples of milk from three farms were in agreement to the quality parameters required by law. In the BMT, only the protein content has not reached the minimum value established by law, and counting was performed for AM (&gt;105 colony forming units (CFU) mL-1) and psychrotrophic bacteria (2.8x106CFU mL-1).  Pseudomonas spp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychrotrophic; Quality; Enzymes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100451

Bacillus subtilis improves maize tolerance to salinity
Provedor de dados: 65
Autores: Ferreira,Nathalia Calhabeu; Mazzuchelli,Rita de Cassia Lima; Pacheco,Ana Claudia; Araujo,Fabio Fernando de; Antunes,Jadson Emanuel Lopes; Araujo,Ademir Sergio Ferreira de.
ABSTRACT: The aim of this study was to evaluate the biochemical responses of maize, under saline stress, inoculated with Bacillus subtilis. Four levels of salinity were assessed: 0mM, 50mM, 100mM, and 200mM of sodium chloride (NaCl). Saline conditions influenced negatively maize growth. However, the inoculation of B. subtilis improved the plant growth at highest level of NaCl. Chlorophyll content decreased while proline increased in inoculated plants submitted to highest salt levels. Also, B. subtilis increased the relative water content in leaves. B. subtilis improves the plant growth under salinity and ameliorates the biochemical damages in maize.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; PGPB; Biochemical responses; Enzymes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000800202

Interaction between xylanase, glucose oxidase and ascorbic acid on the technological quality of whole wheat bread
Provedor de dados: 65
Autores: Silva,Camila Batista da; Almeida,Eveline Lopes; Chang,Yoon Kil.
ABSTRACT: This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whole wheat bread; Enzymes; Oxidants; Texture; Response surface methodology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202249

Cold induced changes on sugar contents and respiratory enzyme activities in coffee genotypes
Provedor de dados: 65
Autores: Partelli,Fábio Luiz; Vieira,Henrique Duarte; Rodrigues,Ana Paula Dias; Pais,Isabel; Campostrini,Eliemar; Chaves,Maria Manuela Coelho Cabral; Ramalho,José Cochicho.
The present research aimed to characterize some biochemical responses of Coffea canephora (clones 02 and 153) and C. arabica (Catucaí IPR 102) genotypes subjected to low positive temperatures, helping to elucidate the mechanisms involved in cold tolerance. For that, one year old plants were subjected successively to 1) a temperature decrease (0.5°C a day) from 25/20°C to 13/8°C (acclimation period), 2) a three day chilling cycle (3x13/4°C) and to 3) a recovery period of 14 days (25/20°C). In Catucaí (less cold sensitive when compared to clone 02) there was an increased activity in the respiratory enzymes malate dehydrogenase and pyruvate kinase. Furthermore, Catucaí showed significant increases along the cold imposition and the higher absolute values after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee tree; Coffea sp.; Sucrose; Enzymes; Cold stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400006

La ecotoxicidad y el pasto japonesa (Leersia sp.) en la fitorremediación de un Gleysol contaminado con petróleo fresco, intemperizado y sulfatos.
Provedor de dados: 32
Autores: Arias Trinidad, Alfredo.
El estudio de ecotoxicidad y fitorremediación de un Gleysol (GL) contaminado con petróleo fresco (PF) e intemperizado (PI) y sulfatos solubles (S-SO4), se realizo para seleccionar indicadores de toxicidad biológicos, evaluar el potencial del pasto japonesa (PJ) en la descontaminación de estos suelos, la presencia de S-SO4 en su interacción con los hidrocarburos, las bacterias sulfato reductoras y actividad enzimática de la arilsulfatasa (ASA). Dos GLs se usaron para establecer los bioensayos (BIO). El BIO1 con nueve tratamientos [T1: 2 607 (biogénico), T2: 6 000, T3: 10 000, T4: 30 000, T5: 60 000, T6: 90 000, T7: 120 000. T8: 150 000 y T9: 180 000 mg kg-1 suelo seco de hidrocarburos totales de petróleo fresco (HTPF)]. El BIO2 con seis tratamientos [T1: 2...
Palavras-chave: Petróleo; Sulfatos; Bacterias; Enzimas; Degradación; Maestría; Producción Agroalimentaria en el Trópico; Oil; Sulfates; Enzymes; Degradation.
Ano: 2012
URL: http://hdl.handle.net/10521/1762

Diversidad bacteriana en el tracto digestivo de la lombriz de tierra Pontoscolex corethrurus.
Provedor de dados: 32
Autores: Brito Vega, Hortensia.
El objetivo del presente trabajo fue estudiar la diversidad bacteriana en el contenido digestivo de la lombriz de tierra endogea Pontoscolex corethrurus. El muestreo de la especie P. corethrurus se realizó en dos localidades de Veracruz, México: Plan de las Hayas, zona ganadera con suelo arcilloso y La Mancha, zona de reserva ecológica y con suelo arenoso. El intestino de las lombrices fue dividido en cuatro secciones (A, B, C y  D). Bajo condiciones asépticas, se prepararon diluciones decimales con el contenido intestinal  empleando el medio líquido Brain Heart Infusion. Posteriormente, la suspensión del contenido intestinal se sembró en medios específicos. La identificación taxonómica de los aislamientos bacterianos puros  se realizó por dos...
Palavras-chave: Anisosimbiosis; Lombriz de tierra; Enzimas; Biodiversidad; Earthworm; Enzymes; Biodiversity; Doctorado; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/265

Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
Provedor de dados: 69
Autores: Martínez,Claudio; Gertosio,Cecilia; Labbe,Angelica; Pérez,Rafael; Ganga,Maria Angélica.
Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.
Tipo: Journal article
Palavras-chave: Alpha-L-arabinofuranosidase yeast; Enzymes; Wine.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009

Thermotolerant fermenting yeasts for simultaneous saccharification fermentation of lignocellulosic biomass
Provedor de dados: 69
Autores: Choudhary,Jairam; Singh,Surender; Nain,Lata.
Lignocellulosic biomass is the most abundant renewable source of energy that has been widely explored as second-generation biofuel feedstock. Despite more than four decades of research, the process of ethanol production from lignocellulosic (LC) biomass remains economically unfeasible. This is due to the high cost of enzymes, end-product inhibition of enzymes, and the need for cost-intensive inputs associated with a separate hydrolysis and fermentation (SHF) process. Thermotolerant yeast strains that can undergo fermentation at temperatures above 40°C are suitable alternatives for developing the simultaneous saccharification and fermentation (SSF) process to overcome the limitations of SHF. This review describes the various approaches to screen and develop...
Tipo: Journal article
Palavras-chave: Bioethanol; Biofuel; Enzymes; Genome shuffling; Lignocellulosic biomass.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300013

Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae)
Provedor de dados: 74
Autores: Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F.
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005

Application forms and mode of action of biocontroller in the management of Meloidogyne incognita in tomato
Provedor de dados: 75
Autores: Frederic,Jean B; Costa,Cândido A da; Rocha,Fernando da S; Fernandes,Maria de Fátima G.
ABSTRACT Mixture of antagonist agents and form of application may influence in the control of root-knot nematodes. Thus, the aim of this work was to study the action of a biological product based on enzyme mixtures, Bacillus sp. and Trichoderma sp., at different concentrations on hatching, motility, mortality and reproduction of Meloidogyne incognita in tomato, considering two ways of application of the product. Eggs and second-stage juveniles (J2) of M. incognita were placed in biological product solutions at concentrations: 0, 1.25, 2.5, 5, 10, 20 and 40 g L-1. The same concentrations were applied to planting pits or to the soil surface. Afterwards, 3,692 eggs of M. incognita were inoculated in tomato crop. A significant reduction in J2 hatching of M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacillus spp.; Trichoderma longibrachiatum; Root-knot nematodes; Enzymes; Biological control.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300254

Действие цинка на активность супероксиддисмутазы и каталазы у Spirulina platensis (Nordst) geitl.
Provedor de dados: 13
Autores: Шахматова, О. А.
Изучали влияние двухвалентного цинка на активность антиоксидантных ферментов супероксиддисмутазы и каталазы у одноклеточной синезеленой водоросли Spirulina platensis. Двухвалентный цинк в концентрациях 0,09 - 9,0 мг/л угнетающе действовал на активность антиоксидантных ферментов  супероксиддисмутазы и каталазы у спирулины. Зависимость активности каталазы и супероксиддисмутазы от концентрации цинка выражалась формулой y=а/(1+bх), где a=0,52 и b=5,28 для каталазы и а=0,49 и b=4,79 для супероксиддисмутазы, что подтверждает факт согласованности действия данных энзимов.
Tipo: Journal Contribution
Palavras-chave: Enzymes; Protein; Zn; Антиоксидантные ферменты; Спирулина Enzymes Enzymes http://aims.fao.org/aos/agrovoc/c_2603.
Ano: 2002
URL: http://repository.ibss.org.ua/dspace/handle/99011/390

Активность ферментов пероксидного комплекса тканей мидии Mytilus galloprovincialis Lam в норме и в условиях естественного окислительного стресса
Provedor de dados: 13
Autores: Гостюхина, О. Л.; Солдатов, А. А.; Головина, И. В.
Исследовано содержание продуктов перекисного окисления липидов (ПОЛ) и состояние ферментативного пероксидного комплекса (ПК) (глутатионпероксидазы (ГП), каталазы и пероксидазы) в гепатопанкреасе, жабрах и ноге черноморского моллюска Mytilus galloprovincialis Lam. в условиях физиологического покоя и нереста. Последнее рассматривали как модель естественного окислительного стресса. Изучали особей двух цветовых морф - черной и коричневой. Выявлена тканевая специфика активностей ферментов и распределения продуктов ПОЛ в состоянии нормы, а также особенности ответных реакций тканей моллюсков обеих морф при действии окислительной нагрузки. Наиболее чувствительной к действию перекисных продуктов в условиях естественного окислительного стресса оказались особи черной...
Tipo: Journal Contribution
Palavras-chave: Ферменты; Каталаза; Пероксидаза; Глутатионпероксидаза; Перекисное окисление липидов; Окислительный стресс; Мидии; Цветовые морфы; Нерест; Enzymes; Catalase; Peroxidase; Glutathione peroxidase; Lipid peroxidation; Spawning; Oxidative stress; Mussels; Color morphs Spawning Enzymes Lipids Spawning Enzymes Lipids http://aims.fao.org/aos/agrovoc/c_14490 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_4362.
Ano: 2005
URL: http://repository.ibss.org.ua/dspace/handle/99011/226

Comparação de duas soluções extratoras na resolução de cinco sistemas enzimáticos em folhas jovens de cupuaçuzeiro (Theobroma grandiflorum Schum.).
Provedor de dados: 14
Autores: COSTA, M. R.; GAIA, J. M. D.; POLTRONIERI, M. C.
Tipo: Folhetos
Palavras-chave: Extração de enzima; Eletroforese de isoenzima; Substância extratora; Isoenzymes; Cupuaçu; Folha; Theobroma Grandiflorum; Analytical methods; Enzymes; Electrophoresis; Leaves.
Ano: 1999
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/377275

Guia prático para caracterização de enzimas.
Provedor de dados: 14
Autores: PACHECO, T. F.; MENDES, T. D.
Neste guia serão abordados os temas mais básicos que integram o estudo da cinética e termodinâmica de reações enzimáticas e, partindo de estudo hipotético, será demonstrado como planejar os experimentos e obter as informações requeridas de forma prática.
Tipo: Livros
Palavras-chave: Termodinâmica; Enzima; Atividade Enzimática; Energia Cinética; Enzymes; Kinetics; Thermodynamics.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1131161

Metodo de purificacao de uma endoquitinase de Trichoderma harzianum com atividade sobre a parede celular de fungos fitopatogenicos.
Provedor de dados: 14
Autores: LIMA, L. H. C.; DE MARCO, J. L.; QUEIROZ, P. R.; ULHOA, C. J.; FELIX, C. R.
Tipo: Folhetos
Palavras-chave: Controle Biológico; Enzima; Trichoderma Harzianum; Biological control; Enzymes.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/180559

Aplicação e pontos críticos no uso de enzimas para monogástricos.
Provedor de dados: 14
Autores: KRABBE, E. L.
Tipo: Artigo na mídia
Palavras-chave: Nutrição animal; Enzima; Dieta; Animal nutrition; Enzymes; Diet.
Ano: 2013
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/972199

Caracterização bioquímica de germoplasma de fruteiras.
Provedor de dados: 14
Autores: MARTINS, C. da S.; POLTRONIERI, M. C.; GAIA, J. M. D.; IKETANI, H.; KAJITA, T.; YOSHIOKA, H.
Convênio Embrapa Amazônia Oriental/JICA-1990-1997.
Tipo: Parte de livro
Palavras-chave: Pimenta-do-reino; Urucu; Isoenzymes; Bixa Orellana; Cupuaçu; Enzima; Germoplasma; Isoenzima; Piper Nigrum; Theobroma Grandiflorum; Enzymes; Germplasm.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/395077

Enzymes: Bioprospecting of fungi that produce enzyme complexes.
Provedor de dados: 14
Autores: FAVARO, L. C. de L.
Elite strains of endophytic fungi, isolated from the Brazilian biodiversity, producers of efficient enzyme complexes for hydrolysis of the pre-treated sugarcane bagasse.
Tipo: Folders
Palavras-chave: Sugarcane bagasse; Enzymes; Fungi; Hydrolysis.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133089

Macauba: Obtaining of macauba pulp oil by aqueous extraction enzyme assisted process.
Provedor de dados: 14
Autores: FAVARO, S. P.
Process of extraction of oil from macauba pulp from fresh fruits, using commercial enzymatic cocktails. The technology consists in extracting oil from macauba pulp using water and enzymes that increase the efficiency of the process without compromising the quality of the oil.
Tipo: Folders
Palavras-chave: Aqueous process; Macaúba; Pulp; Oils; Enzymes.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133080

Definição de protocolos de eletroforese para análise de isoenzimas em pimenta-do-reino (piper nigrum L.).
Provedor de dados: 14
Autores: OLIVEIRA, M. R. C. de; POLTRONIERI, M. C.; MARTINS, C. da S.; GAIA, J. M. D.
Tipo: Folhetos
Palavras-chave: Pimenta-do-reino; Extração de enzima; Macador genético; Eletroferese; Gel de amido; Gel poliacriamida; Pará; Brasil; Isoenzymes; Electropharesis; Isoenzima; Piper Nigrum; Amazonia; Enzymes; Genetic markers.
Ano: 1998
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/375197

Distribuicao da atividade enzimatica no perfil de um latossolo vermelho amarelo de cerrado sob vegetacao nativa e sob sistemas de plantio direto e convencional.
Provedor de dados: 14
Autores: MENDES, I. de C.; REIS JUNIOR, F. B. dos; P. NETO, J. V.
Tipo: Folders
Palavras-chave: ECOSOL160; Zero tillage; Bioquímica do Solo; Cerrado; Enzima; Perfil do Solo; Plantio Direto; Biochemistry; Enzymes; Soil; Soil profiles.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567257

Indicadores biológicos em solos de propriedades rurais sob plantio direto e convencional na região de Rio Verde-GO.
Provedor de dados: 14
Autores: MENDES, I. de C.; SCOPEL, E.; REIS JÚNIOR, F. B.; MARCHETTI, A. D.
Tipo: Folders
Palavras-chave: Enzymic activity; Biologia do Solo; Atividade Enzimática; Cerrado; Enzima; Enzymes; Soil biology.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567690

Effects of cover crops species on Passiflora edulis nutrition.
Provedor de dados: 14
Autores: ANDRADE, L. R. M. de; CARVALHO, A. M. de; MENDES, I. de C.; VIVALDI, L.; KARIA, C. T.; JUNQUEIRA, N. T. V.
Tipo: Folders
Palavras-chave: Cover plants; Enzima; Maracujá; Nutrição Vegetal; Planta de Cobertura; Produtividade; Solo; Crop yield; Enzymes; Plant nutrition; Soil.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567640

Atividade enzimática de um latossolo vermelho de cerrado sob sistemas integrados lavoura-pastagens.
Provedor de dados: 14
Autores: SILVA, L. G.; MENDES, I. de C.; ROLIM, L. F. S.; SANTOS, A. B.; REIS JÚNIOR, F. B.; VILELA, L.
Tipo: Folders
Palavras-chave: Microbial flora; Bioquímica do Solo; Biomassa; Cerrado; Enzima; Microbiologia do Solo; População Microbiana; Biochemistry; Biomass; Enzymes; Microbiology; Soil.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569446

Une utilisation des enzymes proteolytiques extraites des visceres de poissons: La coagulation du lait.
Provedor de dados: 5
Autores: Guerard, F.
Chronic shortages in rennet have led to the use of enzyme preparations  from various biological origins and capable of undergoing a milk-clotting process similar to that catalyzed by calf rennet. A proteolytic enzyme extracted from the gastric mucosa of dogfish Scyliorhinus canicula exhibits a clotting activity and several physical properties, which render it suitable for utilization in cheese-making industry.
Tipo: Text
Palavras-chave: Scyliorhinus canicula; Food technology; Enzymes; Shark utilization; Fishery products.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/publication-1781.pdf

Growth factors in Penaeid shrimp feeding
Provedor de dados: 5
Autores: Guillaume, Julien; Cruz, R; Cuzon, Gerard; Wormhoudt Van, A; Revol, A.
Some nutrients known as non essential in vertebrates could be essential in invertebrates and could be related to some "unknown growth factors" which were evidenced or suspected, mainly in mollusc or crustacean flesh or meal, by several authors working on different species of crustaceans. A set of experiments was carried out in our laboratory in order to isolate one of these factors found in squid meal.
Tipo: Text
Palavras-chave: Penaeus japonicus; Food absorption; Digestive system; Haemolymph; Amino acids; Glucose; Digestion; Enzymes; Experimental research; Feeding; Shrimp culture; Laboratory culture.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1464.pdf

Effet de l'incorporation de farines de soja et de poisson dans l'aliment sur la croissance et les enzymes digestives de Penaeus vannamei
Provedor de dados: 5
Autores: Galgani, Francois; Ceccaldi, Hubert J.; Aquacop, Aquacop.
In order to improve the formulation of diets fed to reared Penaeus vannamei, the effects of soya bean and fish meals on the growth, survival, and the characteristics of the hepatopancreas and digestive enzymes of the species were studied. Four soya bean meal concentrations (0, 5, 15, 40%), four fish meal concentrations (0, 5, 15, 30%), two forms of soya bean meal and three fish meals of different geographical origin were tested. Experimental conditions were as follows: ten individuals were placed in each tank (0.5 m2 ) and two tanks were subjected to each treatment. Food was given twice a day (08-09.00 h and 19.00 h) at 10% of the initial weight each day. For biochemical analyses, nine individuals per treatment were used for the determination of the...
Tipo: Text
Palavras-chave: Aquaculture; Nutrition; Pénéidés; Soja; Farine de poisson; Enzymes; Enzymes digestives.; Aquaculture; Nutrition; Penaeidae; Soya bean; Fish meal; Digestive enzymes.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00135/24597/22626.pdf

Caractérisation morphologique et fonctionnelle des hémocytes chez l'huître plate, Ostrea edulis
Provedor de dados: 5
Autores: Xue, Quiggang.
The mechanism that bivalve molluscs react to infectious agents are important research subjects in the fIeld of disease control studies for these animals. While the selection of disease resistant populations have made decisive progression in some species, the mechanism behinds these resistant phenomena is not yet clear so far. However, hemocytes must represent one of the key factors in the reaction process. Based on this hypothesis, the structure and function of European flat oyster, Ostrea edulis, hemocytes have been investigated in aid of different techniques in the present study. ln addition, Pacific oyster, Crassostrea gigas, hemocytes have been analysed in certain aspects so as to provide some results for comparison. European flat oyster and Pacific...
Tipo: Text
Palavras-chave: Bivalvia; Ostrea edulis; Crassostrea gigas; Mécanismes de défense; Hémocytes; Granulocytes; Hyalinocytes; Séparation; Anticorps monoclonaux; Enzymes; Cytométrie de flux; Bivalvia; Ostrea edulis; Crassostrea gigas; Defence mechanisms; Hemocytes; Granulocytes; Hyalinocytes; Separation; Monoclonal antibodies; Enzymes; Flow cytometry.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00107/21820/19406.pdf

A brief review of nutritional studies for Penaeus monodon
Provedor de dados: 5
Autores: Liao, I; Liu, F.
In order to develop effective formulated for Penaeus monodon , sufficient information is needed on its nutritional requirements. Unfortunately, research on this area has been very limited. This paper outlines the results of such research. Since data on the nutritional requirements of P. japonicus are relatively well established, some of these data are also reviewed in this paper and are used as a basis for assessing the nutritional requirements of P. monodon . Protein, lipid, carbohydrate, mineral and vitamin requirements are evaluated from the parameters of growth survival and feed efficiency. Studies on the effects of enzymes and the development of microparticulate diet for larvae are also summarized.
Tipo: Text
Palavras-chave: Penaeus monodon; Pond culture; Shrimp culture; Amino acids; Fatty acids; Marine crustaceans; Diets; Enzymes; Feed efficiency; Survival; Growth; Vitamins; Carbohydrates; Lipids; Proteins.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1471.pdf

Anatomy and physiology of digestive tract of crustaceans decapods reared in aquaculture
Provedor de dados: 5
Autores: Ceccaldi, H.
The digestive tract of crustacea is complex. It is composed of a shor t oesophagum, a stomach with two bags, and internal wall with appendags specialized in grinding of feeds. These hard mastication pieces represent the gastric mill. Setae, filters included in the stomach structure allow the separation between food particles and liquid compounds. Those one are coming over through filters and more on to the digestive gland tubules or mid gut gland. In this organ, several types of cells play specialized functions: absorption, enzyme secretion, stocking function. Some cells of a new type, with undefined function, may be neurosecretions function, have been described recently. The end of tubules of digestive gland exhibits cells with embryonic characteristics....
Tipo: Text
Palavras-chave: Decapoda; Neurosecretion; Feed; Rearing; Digestive glands; Enzymes; Stomach; Crustacean culture.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1486.pdf

Fonctionnalisation de surfaces d'acier inoxydable par des enzymes en vue d'inhiber l'adhésion de bactéries et la formation de biofilms en eau de mer
Provedor de dados: 5
Autores: Caro, A.; Humblot, V.; Minier, M.; Salmain, M.; Compère, Chantal; Pradier, C.-m.
All surfaces that are immersed in seawater rapidly become covered by nitrogenous compounds, proteins, carbohydrates, salts, silica and bacteria in a few hours, and by a grown biofilm in a few days. These biodeterioration, physical and chemical damages involve important cleaning costs. Organostanic paintings used up to now to protect the surfaces against this spoiling will be prohibited in 2008 because of their toxicity. That's why the stake today is to find alternatives to these paintings. The solution which is here suggested consists in acting on the first stages of the formation of the biofilm i.e. the proteins and bacterial adsorption on the surface. For this, the surface is chemically modified then functionalized by enzymes. Enzymes will act locally on...
Tipo: Text
Palavras-chave: Acier inoxydable; Fonctionnalisation; Enzymes; Adsorption bactérienne; PM-IRRAS; XPS; Stainless steel; Functionalization; Enzymes; Bacterial adsorption; IR; XPS.
Ano: 2006
URL: https://archimer.ifremer.fr/doc/00479/59095/61733.pdf

Testing Protein Digestibility in Red Grouper Epinephelus Morio using In Vitro and In Vivo Methods
Provedor de dados: 5
Autores: Silva, A. F.; Escalante, Karla; Alvarez-gonzalez, C. A.; Guerrero-olazaran, M.; Cuzon, Gerard; Gaxiola, Gabriela.
The digestibility of pre-selected ingredients was evaluated for Epinephelus morio using pH-stat and multi-enzyme extract (stomach, intestine, and ceca). The degree of hydrolysis (DH) was tested with acid and alkaline proteases using crustacean meals, squid meal, fish protein concentrate (FPC), fish meal, dried whey, as well as canola paste (w/or w/o phytase), soybean meal (w/or w/o phytase), soyprotein concentrate (SPC), and wheat gluten. SPC produced DH values between 3.6-4.1%, obtained from the three different parts of the digestive tract. Canola paste produced high DH in the stomach by adding phytase to 0.8 and 2% feed. This process was repeated under alkaline conditions for DH, with 0.6-2.6% in the pyloric ceca, and 1-2.2% in the intestine. The DH from...
Tipo: Text
Palavras-chave: ADC; In vitro digestibility; Enzymes; Fish; Feedstuffs.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00248/35930/80189.pdf

Effects of commercial enzymes on the adhesion of a marine biofilm-forming bacterium
Provedor de dados: 5
Autores: Leroy, C.; Delbarre Ladrat, Christine; Ghillebaert, F.; Compere, Chantal; Combes, D.
The antifouling potential of commercial hydrolases, four proteases, seven glycosidases and one lipase was evaluated on the adhesion of marine Pseudoalteromonas sp. D41. The experimental method, adapted to screen antifouling agents, was based on bacterial adhesion in natural sterile sea water in a microtiter plate and on total biomass quantification by the fluorescent dye DAPI (4[prime]6-diamidino-2-phenylindole). Savinase (subtilisin) was the most effective hydrolase in both the prevention of bacterial adhesion and the removal of adhered bacteria. However, some enzymatic preparations tested such as Amano protease were not only ineffective but also increased the number of adhered bacterial cells. Enumeration using epifluorescence microscopy of CTC...
Tipo: Text
Palavras-chave: Marine biofilm; Antifouling; Pseudoalteromonas; Enzymes; Adhesion.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6140.pdf

Formaldehyde preservation for deferred measurements of alkaline phosphatase activities in marine samples
Provedor de dados: 5
Autores: Labry, Claire; Urvoy, Marion.
Alkaline phosphatases are the main enzymes required by microorganisms to hydrolyse organic phosphorus into available phosphate in aquatic environments. The investigations of alkaline phosphatase activity (APA) usually generate numerous samples (size fractionation, Michaelis-Menten kinetics). Therefore, convenient and reliable preservation of incubated samples for a deferred analysis would be very useful when measurements cannot be performed right away. The APA of marine pond waters was measured using 4-Methylumbelliferyl phosphate (MUF-P) as the fluorogenic substrate modelling natural organic phosphorus compounds. Where typical inhibitors of other enzymatic activities, such as 1% sodium dodecyl sulfate, mercuric chloride, or buffered solutions of ammonium...
Tipo: Text
Palavras-chave: Alkaline phosphatase activity; Formaldehyde; Inhibition; Enzymes; Aquatic studies; Deferred measurements.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00656/76787/78211.pdf

Relations between bacterial extracellular enzyme activities and heterotrophic substrate uptake in a brackishwater environment.
Provedor de dados: 5
Autores: Hoppe, H.
Extracellular enzymes mediate the decomposition of polymeric organic compounds in natural waters. In many cases these enzymes comprise a component linked to the bacterial fraction of the aquatic community and react in close association with these cells. An annual survey of microbial activities in a brackish water fjord exhibited an excellent correlation between V sub(m) of proteases (ability to split the substrate analogue methylumbelliferyl-leucine) and V sub(m) as well as T sub(R) for leucine uptake by microorganisms. Hydrolysis rates (H sub(R)) for the decomposition of naturally occurring competitive substrate analogues of the model substrates (methylumbelliferyl-(MUF)-leucine, MUF- alpha -D-glucose, MUF-N-acetyl-glucosamine, MUF-phosphate) drastically...
Tipo: Text
Palavras-chave: Biogeochemistry; Fjords; Bacteria; Brackishwater environment; Seasonal variations; Dissolved organic matter; Interspecific relationships; Hydrolysis; Enzymes.
Ano: 1984
URL: http://archimer.ifremer.fr/doc/1984/acte-952.pdf

Variations des activites digestives en fonction des facteurs du milieu chez les crustaces
Provedor de dados: 5
Autores: Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J.
Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases.
Tipo: Text
Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf

Extraction de lipides en voie aqueuse par bioréacteur enzymatique combine à l'ultrafiltration : application a la valorisation de co-produits de poisson (Sardina pilchardus)
Provedor de dados: 5
Autores: Dumay, Justine.
The main goal of this work was to up-grade sardine (Sardina pilchardus) by-products using mild procedure, environmentally sound, in order to obtain valuable compounds involving moderate investment and low energy consumption.   Hydrolysis and ultrafiltration techniques have been investigated. Firstly, selected enzymes have been calibrated in order to determine their optimal conditions with a model substrate and to permit the comparison between them. Then, hydrolysis on sardine head and viscera have been carried out in two steps. The first one had the objective to determine the enzyme efficiency and the study area. The second step was to optimise enzymatic hydrolysis using experimental designs. The aim of this second step was to obtain the highest lipid...
Tipo: Text
Palavras-chave: Up grading; By products; Ultrafiltration; Enzymes; Lipids; Sardina pilchardus; Valorisation; Co produits; Ultrafiltration; Enzymes; Lipides; Sardina pilchardus.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/these-1556.pdf

Early weaning of marine fish larvae onto microdiets: Constraints and perspectives
Provedor de dados: 5
Autores: Person, L.
Weaning small sized marine fish larvae at first feeding directly onto compound pellets is still difficult, while good results can be obtained when used in combination with live prey or when a prefeeding period on live prey is provided up to a size of about 2-3 mg. Microdiets are generally well ingested as their acceptability and visual detection may be improved using feeding activators and increasing good perception contrast. In theory, food may be processed to have a correct nutritional balance, selection of high digestible components is very important as larval digestive system is not fully functional during the first 2 or 3 weeks of life.
Tipo: Text
Palavras-chave: Hatching; Fatty acids; Amino acids; Nutritional requirements; Feeding behaviour; Stomach; Digestive system; Enzymes; Digestion; Physiology; Diets; Pellet feeds; Feeding; Fish larvae.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/1989/acte-1422.pdf

Evaluating the effects of formulated moist diets on juveniles of Patagonian Octopus Enteroctopus megalocyathus (Gould 1852)
Provedor de dados: 5
Autores: Farias, Ana; Pereda, Sandra V.; Uriarte, Iker; Doerner, Jessica; Cuzon, Gerard; Rosas, Carlos.
The aim of this study was to evaluate the performance of Patagonian octopus fed with moist diets formulated with several local feed ingredients. All formulated diets were based on crab paste (70%) and the experimental feed ingredient (30%). Experiment I assayed salmon meal, prime sardine meal, and wheat gluten, using fresh fish as a control; experiment 2 assayed prime fish meal and macroalgal meal against crab paste alone as a control. The ingestion rate was lower than expected for all diets except those of fresh fish, crab paste alone, and crab paste plus prime sardine meal. No significant differences were found in the observed digestibility of the diets, indicating, in general, low digestibility, even for fresh fish. The highest protease values were...
Tipo: Text
Palavras-chave: Octopus; Emeroctopus megalocyathus; Digestibility; Enzymes; Consumption rate; Nutrition; Moist diets.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00030/14106/11342.pdf

Ultrastructure et activite de la glande salivaire de Patella safiana LMK (Mollusque, Gasteropode)
Provedor de dados: 5
Autores: Benmeradi, S; Benmeradi, N.
The ultrastructural study of the Patella safiana salivary gland reveals two types of cells: the dark cells containing round-shaped secretory vesicles of lamellar structure and the light cells which have homogenous and fibrous secretory granules that are star-shaped sometimes. The two types of cells have the cytological characteristics of high proteosynthetic activity cells.
Tipo: Text
Palavras-chave: Patella safiana; Gastropoda; Biochemical analysis; Secretory organs; Cytology; Cells; Marine molluscs; Enzymes; Ultrastructure.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1712.pdf

Effet des hautes pressions sur les indicateurs de maturation de la viande et d'altération du muscle de poisson
Provedor de dados: 5
Autores: Cheret, Romuald.
This study deals with the influence of the high pressure process on the ageing of meat and the degradation of the fish muscle. The aim is to characterize the effect of the high pressure treatment on the protease activities responsible for degradation for the sea bass muscle (Dicentrarchus labrax  L.) and bovine muscle (Biceps femoris), but also on the texture, the colour and the microstructure. The samples were pressurized between 0 and 500 MPa during 5 minutes, and were stored at 4°C. The different proteasic systems involved in the post-mortem degradation of the muscle (mainly cathepsins and calpains) were quantified after the high pressure treatment and during the post-mortem storage. The results shown that the sensitivity of the proteases depended on...
Tipo: Text
Palavras-chave: Enzymes; Muscular proteins; Bovine muscle; Fish muscle; Proteases; High pressure; Protéines musculaires; Enzymes; Viande bovine; Muscle de poisson; Protéases; Hautes pressions.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-553.pdf

La bonamiose: caractérisation du parasite Bonamia ostreae et étude des ses interactions avec l'hôte, l'huître plate Ostrea edulis
Provedor de dados: 5
Autores: Besnard-cochennec, Nathalie.
La bonamiose, maladie due au protozoaire Bonamia ostreae a été détectée en 1979 pour la première fois en Bretagne au cours d'épisodes de mortalité. Depuis, cette maladie s'est propagée à tous les centres ostréicoles français, puis européens. Cette étude a pour objectif de décrire les compartiments du modèle B. ostreae/hémocyte de l'huître plates, Ostrea edulis et d'analyser leurs interactions in vivo et in vitro. La caractérisation enzymatique, antigénique et moléculaire du parasite a été réalisée. Ceci a permis de disposer d'une palette de réactifs pour la comparaison de ce parasite avec d'autres protozoaires. Ainsi, un parasite de l'huître creuse chinoise Crassostrea rivularis a té décrit, puis rapproché du genre Bonamia. La morphologie et l'équipement...
Tipo: Text
Palavras-chave: Huître plate; Ostrea edulis; Huître creuse chinois; Crassostrea rivularis; Bonamia ostreae; Enzymes; Résistance; Mécanisme de défense.
Ano: 1997
URL: http://archimer.ifremer.fr/doc/00211/32198/30635.pdf

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development
Provedor de dados: 5
Autores: Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R.
Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA...
Tipo: Text
Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae.
Ano: 2004
URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf

Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas
Provedor de dados: 5
Autores: Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text
Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf

Gongronema latifolium Modulates Rauwolfia vomitoria-Induced Behaviour and Histomorphology of the Cerebral Cortex
Provedor de dados: 77
Autores: Ekong,Moses B; Peter,Aniekan I; Ekpene,Ubong U; Bassey,Enobong I; Eluwa,Mokutima A; Akpanabiatu,Monday I; Ekanem,Theresa B.
Rauwolfia vomitoria (RV) has potent sedative effect, which may result in severe unpleasant consequences if not controlled. This necessitated this study on the effect of Gongronema latifolium (GL) on RV-induced behaviour, biochemical activities, and histomorphology of the cerebral cortex. Eighteen male Wistar rats of average weight 266 g were grouped into three (1­3). Group 1 was the control administered 0.5 mL of Tween®20, while groups 2 and 3 were administered 150 mg/kg of RV, and a combination of 150 mg/kg of RV and 200 mg/kg of GL (RV+GL), respectively for seven days. Twelve hours after treatments, open field neurobehavioral test was carried-out and the animals euthanized. Their sera were analyzed, and their cerebral cortices routinely processed by...
Tipo: Journal article
Palavras-chave: Rauwolfia vomitoria; Gongronema latifolium; Behavior; Enzymes; Lipid; Cerebral cortex; Histomorphology.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100013

ROLE OF ENZYMES IN THE REMEDIATION OF POLLUTED ENVIRONMENTS
Provedor de dados: 126
Autores: Rao,M.A; Scelza,R; Scotti,R; Gianfreda,L.
Environmental pollution is growing more and more due to the indiscriminate and frequently deliberate release of hazardous, harmful substances. Research efforts have been devoted to develop new, low-cost, low-technology, eco-friendly treatments capable of reducing and even eliminating pollution in the atmosphere, the hydrosphere and soil environments. Among biological agents, enzymes have a great potentiality to effectively transform and detoxify polluting substances because they have been recognized to be able to transform pollutants at a detectable rate and are potentially suitable to restore polluted environments. This brief review will examine some classes of pollutants and enzymes capable of transforming them effectively into innocuous products....
Tipo: Journal article
Palavras-chave: Pollutants; Enzymes; Environmental pollution; Bioremediation.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000100008

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom
Provedor de dados: 80
Autores: Tan,NH; Fung,SY; Ponnudurai,G.
Bungarus flaviceps (red-headed krait) venom presents an intravenous LD50 of 0.32 μg/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes with other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bungarus flaviceps venom; Enzymes; ELISA; Neutralization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100014

Pathogenic characteristics of yeasts isolated from vaginal secretion preserved under mineral oil
Provedor de dados: 80
Autores: Severo Gomes,B; Souza Motta,CM; Lima,AN; Porto,ALF.
In order to evaluate the pathogenicity of yeasts isolated from vaginal secretion of pregnant and non-pregnant women - stored in mineral oil at the URM Mycology Collection, Department of Mycology, Federal University of Pernambuco - 30 samples belonging to the genera Candida, Rhodotorula, Trichosporon, and Kloeckera, were studied regarding their pathogenic characteristics, ability to grow at room temperature (28°C ± 1°C), 37°C, and 42°C for 72 hours, and production of both phospholipase and proteinase. Results showed that all 30 isolates (100%) were able to grow at room temperature and 37°C, and that 17 samples (57%) were able to grow at 42°C. Evaluation of enzymatic activity showed protease activity in only two isolates (7%), namely C. maritima and C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity; Yeasts; Enzymes.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400013

Proteome of fraction from Tityus serrulatus venom reveals new enzymes and toxins
Provedor de dados: 80
Autores: Amorim,Fernanda Gobbi; Longhim,Heloisa Tavoni; Cologna,Camila Takeno; Degueldre,Michel; Pauw,Edwin De; Quinton,Loïc; Arantes,Eliane Candiani.
Abstract  Background: Tityus serrulatus venom (Ts venom) is a complex mixture of several compounds with biotechnological and therapeutical potentials, which highlights the importance of the identification and characterization of these components. Although a considerable number of studies have been dedicated to the characterization of this complex cocktail, there is still a limitation of knowledge concerning its venom composition. Most of Ts venom studies aim to isolate and characterize their neurotoxins, which are small, basic proteins and are eluted with high buffer concentrations on cation exchange chromatography. The first and largest fraction from carboxymethyl cellulose-52 (CMC-52) chromatography of Ts venom, named fraction I (Fr I), is a mixture of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tityus serrulatus; Scorpion venom; Enzymes; Proteases; ACE inhibitors; Proteome.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100308

Enzymatic and toxinological activities of Hypnale hypnale (hump-nosed pit viper) venom and its fractionation by ion exchange high performance liquid chromatography
Provedor de dados: 80
Autores: Tan,CH; Sim,SM; Gnanathasan,CA; Fung,SY; Ponnudurai,G; Pailoor,J; Tan,NH.
Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hypnale hypnale venom and venom fractions obtained using Resource Q ion exchange chromatography were determined. The venom exhibited toxic activities typical of pit viper venom, comparable to that of its sister taxon, the Malayan pit viper (Calloselasma rhodostoma). Particularly noteworthy were its high activities of thrombin-like enzyme, proteases, phospholipase A2, L-amino acid oxidase and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypnale hypnale; Venom; Enzymes; Toxins; Fibrinogen; Nephrotoxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400015

Hymenoptera venom review focusing on Apis mellifera
Provedor de dados: 80
Autores: Lima,P. R. de; Brochetto-Braga,M. R.
Hymenoptera venoms are complex mixtures containing simple organic molecules, proteins, peptides, and other bioactive elements. Several of these components have been isolated and characterized, and their primary structures determined by biochemical techniques. These compounds are responsible for many toxic or allergic reactions in different organisms, such as local pain, inflammation, itching, irritation, and moderate or severe allergic reactions. The most extensively characterized Hymenoptera venoms are bee venoms, mainly from the Apis genus and also from social wasps and ant species. However, there is little information about other Hymenoptera groups. The Apis venom presents high molecular weight molecules - enzymes with a molecular weight higher than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Venom; Enzymes; Peptides.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200002

Molecular key to seven Brazilian species of Trichogramma (Hymenoptera: Trichogrammatidae) using sequences of the ITS2 region and restriction analysis
Provedor de dados: 82
Autores: CIOCIOLA JR,AMÉRICO I.; ZUCCHI,ROBERTO A.; STOUTHAMER,RICHARD.
Using the ITS2 sequences of the ribosomal DNA together with some restriction enzymes, a simple and precise molecular key to some Brazilian species of Trichogramma was created. This key is very easy to make and quick results can be obtained on the identification of this minute parasitoid (0.25 mm). The methodology presented is easily implemented and can be used to detect possible contaminations under laboratory rearings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; DNA; Enzymes; Biological control.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000200008

Electrophoretic protein pattern and acid phosphatase activity in the midgut extracts of Apis mellifera L. (Hymenoptera: Apidae) during metamorphosis
Provedor de dados: 82
Autores: Cavalcante,Vagner M.; Cruz-Landim,Carminda da.
The electrophoretic pattern of proteins and the activity of acid phosphatase were described in the midgut extracts during the post-embryonic stages of Apis mellifera L. in order to stablish a correlation with the metamorphosis events. The results show the greatest variation in proteic electrophoretical bands during prepupal stage and the smallest variation during the brown eyed pupae stage. The acid phosphatase activity was higher during the larval last instar and lower in white eyed pupae. The greatest variety of bands during the prepupal stage coincides with the histolysis of the larval midgut epithelium and the lowest variability in the brown eyed pupa coincides with the end of the midgut differentiation. In another hand, the greatest phosphatase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differentiation; Enzymes; Histolysis; Larva; Prepupa; Post-embryonic development; Pupa.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200007

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures
Provedor de dados: 83
Autores: Tuzuki,Bárbara Louise L.; Delunardo,Frederico Augusto C.; Ribeiro,Luciana N.; Melo,Caroliny P. de; Gomes,Levy Carvalho; Chippari-Gomes,Adriana Regina.
ABSTRACT This study evaluates the effects of exposure to manganese (Mn2+) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; DNA damage; Enzymes; Genotoxicity; Metal; Micronuclei.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000400202

SERUM BIOCHEMICAL CHANGES IN EXPERIMENTAL GAMBIAN TRYPANOSOMOSIS I. ENZYMES AND ELECTROLYTES
Provedor de dados: 19
Autores: Abenga, J. N.; Anosa, V. O.
Palavras-chave: Gambian trypanosomosis; Enzymes; Electrolytes; Trypanosoma brucei gambiense; Monkeys.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/643

Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut
Provedor de dados: 30
Autores: Manjula, K.
Palavras-chave: Biological control; Biochemical compounds; Bacteria; Poultry equipment; Fungi; Enzymes; Fungicides; Antibiotics; Seed treatment; Chitin.
Ano: 2004
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3322

Manual of laboratory procedures for quality evaluation of sorghum and pearl millet
Provedor de dados: 30
Autores: M. I.
Palavras-chave: Sampling; Flours; Distilling; Husking; Grain; Sorghum; Enzymes; Millets; Bakery products.
Ano: 1997
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4197

Groundnut bacterial wilt: past, present, and future
Provedor de dados: 30
Autores: V.K.
Palavras-chave: Bacteria; Groundnuts; Pathogens; PCR; DNA; Enzymes; Genotypes; Oil crops; Mutants.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4181

Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera
Provedor de dados: 30
Autores: Arora, R.,( Richa Arora ).
Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230

Assessing joint research impacts
Provedor de dados: 30
Autores: M.C.S.
Palavras-chave: Pigeonpeas; Chickpeas; Millets; Sorghum; Hybrids; Germplasm; Genetic soil types; Enzymes; Groundnuts; Double cropping.
Ano: 1998
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4201

Development of cost-effective SNP assays for chickpea genome analysis and breeding
Provedor de dados: 30
Autores: Varshney, R.K.,( Rajeev K. Varshney ).
Palavras-chave: Genomes; Polymorphism; Genotypes; Enzymes; Nucleotides; Chickpeas; DNA; Bioinformatics; Genetic structures; Genes.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3282

International Chickpea and Pigeonpea Newsletter
Provedor de dados: 30
Autores: Jagdish.
Palavras-chave: Chickpeas; Pigeonpeas; Enzymes; Genotypes; Integrated pest management; Grafting; Biochemical compounds; Grain legumes; Sowing date; Abiotic injuries.
Ano: 2002
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4648

CHICKPEA BIBLIOGRAPHY
Provedor de dados: 30
Autores: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Palavras-chave: Chickpeas; Grain legumes; Amino acids; Peas; Proteins; Enzymes; Sowing rates; Vitamins; Wheats; Biosynthesis.
Ano: 1977
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3940

Nitrogen Nutrition of Groundnut in Alfisols
Provedor de dados: 30
Autores: P.T.C.
Palavras-chave: Root nodulation; Groundnuts; Nitrogen fixation; Genotypes; Rhizobium; Bacteria; Nitrogen; Enzymes; Application methods; Legumes.
Ano: 1990
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4084

Environmental and physiological factors that affect the efficacy of herbicides that inhibit the enzyme protoporphyrinogen oxidase: a literature review
Provedor de dados: 87
Autores: Matzenbacher,F.O.; Vidal,R.A.; Merotto Jr.,A; Trezzi,M.M.
Herbicides that inhibit the enzyme protoporphyrinogen oxidase (PROTOX) are usually effective to control dicotyledonous weeds and their agronomic efficacy is affected by environmental and physiological factors. The objective of this review is to summarize the knowledge of those factors available in the scientific literature in the last decade. Environmental factors that influence PROTOX inhibitors include temperature, irradiance and relative humidity. The most relevant physiological factors are the activity of enzymes that can detoxify herbicides and also of enzymes that mitigate the effects of oxidative stress in plants. The study also suggests some possible management strategies that could optimize the activity of PROTOX-inhibiting herbicides.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Temperature; Light; Relative humidity; Enzymes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000200024