Consulta: subjectFacets:"Epitope"
Registros recuperados: 9
Data/hora: 11/06/2024 07:16:02

Characterization of Epitopes on an 18 kDa Piroplasm Surface Protein of Babesia equi
Provedor de dados: 19
Autores: Ali, Shuja; Sugimoto, Chihiro; Kanemaru, Takumi; Kamada, Masanobu; Onuma, Misao.
Palavras-chave: B. equi; Piroplasm; Epitope.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/225

Studies on Epitope Recognition of the Toxoplasma Nucleoside Triphosphate Hydrolase
Provedor de dados: 19
Autores: Johnson, M. S.; Broady, K. W.; Johnson, Alan M.
Palavras-chave: Toxoplasma; NTPase; Epitope.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/327

Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil
Provedor de dados: 55
Autores: Queiroz,Artur Trancoso Lopo de; Santos,Luciane Amorim; Moreau,Domingos Ramon; Oliveira,Tulio de; Watkins,David I.; Galvão-Castro,Bernardo; Alcantara,Luiz Carlos Junior.
Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program (SNAP). The HIVbase epitope mapping...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Env; Vaccine; Epitope.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100008

Prediction of exposed domains of envelope glycoprotein in Indian HIV-1 isolates and experimental confirmation of their immunogenicity in humans
Provedor de dados: 56
Autores: Mohabatkar,H.; Kar,S.K.
We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrophilicity, flexibility, accessibility, inverted hydrophobicity, and secondary structure were considered. We identified several potential antigenic epitopes in a B subtype strain of envelope glycoprotein of HIV-1 (IIIB). Solid- phase peptide synthesis methods of Merrifield and Fmoc chemistry were used for synthesizing peptides. These synthetic peptides corresponded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human immunodeficiency virus; Glycoprotein; Epitope; Immunogenicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500008

Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system
Provedor de dados: 56
Autores: Langella,P.; Le Loir,Y.
Lactic acid bacteria (LAB) are Gram-positive bacteria and are generally regarded as safe (GRAS) organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc) was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein secretion; Staphylococcal nuclease; Lactococcus lactis; Propeptide; Bovine coronavirus; Epitope.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200007

N-terminal amino acids of bovine alpha interferons are relevant for the neutralization of their antiviral activity
Provedor de dados: 56
Autores: Barreto Filho,J.B.; Eiras,P.R.S.; Golgher,R.R.
The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) binds to a linear sequence of residues 10 to 34. We show here that the antiviral activities of these two IFNs were neutralized by the F12 mAb to different extents using two tests. In residual activity tests the antiviral activity dropped by more than 99% with rBoIFN-alphaC and by 84% with rBoIFN-alpha1. In checkerboard antibody titrations, the F12 mAb titer was 12,000...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine alpha interferon; Neutralization; Amino acid; Epitope.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500015

Genetic variations of EBV-LMP1 from nasopharyngeal carcinoma biopsies: potential loss of T cell epitopes
Provedor de dados: 56
Autores: Tang,Y.L.; Lu,J.H.; Cao,L.; Wu,M.H.; Peng,S.P.; Zhou,H.D.; Huang,C.; Yang,Y.X.; Zhou,Y.H.; Chen,Q.; Li,X.L.; Zhou,M.; Li,G.Y.
To find Epstein-Barr virus (EBV) strains with genetic variations of EBV latent membrane protein 1 (EBV-LMP1) from nasopharyngeal carcinoma (NPC), the full-length DNA of LMP1 genes from 21 NPC biopsies obtained in Hunan province in southern China was amplified and sequenced. Our sequences were compared to those previously reported by the Clustal V method. Results showed that all 21 sequences displayed two amino acid changes most frequently in LMP1 of CD4+ T cell epitopes at codons 144 (F®I, 21/21) and 212 (G®S, 19/21) or (G®N, 2/21). We also show that type A EBV strain is prevalent in the cases of NPC from Hunan province with a 30-bp 18/21 deletion, and we highlight that this deletion resulted in loss of one of the CD4+ T cell-restricted epitopes. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variation; Epitope; Immune recognition; Latent membrane protein 1; Tumorigenicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200006

Immunologic cross-reactivity between Muscovy duck parvovirus and goose parvovirus on the basis of epitope prediction
Provedor de dados: 58
Autores: Li,Ming; Yu,Tian-fei.
Through bioinformatic prediction, between Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV), there were one epitope AA503-509 (RANEPKE) on non-structural protein and three epitopes AA426-430 (SQDLD), 540-544 (DPYRS), 685-691 (KENSKRW) on structural protein might cross-react with each other. Furthermore, the four epitops were expressed in Escherichia coli. All the four recombinant proteins could react with GPV-antisera and MDPV-antisera in Western blot.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: MDPV; GPV; Cross-reactivity; Prediction; Epitope.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200031

Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin
Provedor de dados: 58
Autores: Alves,Guilherme Guerra; Machado-de-Ávila,Ricardo Andrez; Chávez-Olórtegui,Carlos Delfin; Silva,Rodrigo Otávio Silveira; Lobato,Francisco Carlos Faria.
Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epsilon; Toxin; Enterotoxemia; Epitope; Mapping.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300570