Consulta: subjectFacets:"Exopolysaccharide"
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Data/hora: 06/06/2024 05:43:31

Computational analysis suggests that virulence of Chromobacterium violaceum might be linked to biofilm formation and poly-NAG biosynthesis
Provedor de dados: 74
Autores: Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques.
Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031

Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology
Provedor de dados: 5
Autores: Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.
The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the...
Tipo: Text
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf

Ultrasonic depolymerization of an exopolysaccharide produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid
Provedor de dados: 5
Autores: Petit, Anne-celine; Noiret, Nicolas; Guezennec, Jean; Gondrexon, N; Colliec-jouault, Sylvia.
Low frequency ultrasound was used to depolymerize a high-molecular-weight exopolysaccharide (EPS) produced by a deep-sea hydrothermal bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. The influence of several parameters was examined including the duration of ultrasonic irradiation, EPS concentration. reaction temperature and volume of the sonicated solution. With the aim of optimizing the depolyrnerization, the native EPS was simultaneously treated with hydrogen peroxide and ultrasound. This study identified the sonication conditions that produce low-molecular-weight derivatives from the native EPS (&gt;10(6) Da) with good reproducibility. (C) 2006 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: Exopolysaccharide; Depolymerization; Ultrasound.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2123.pdf

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection
Provedor de dados: 5
Autores: Zykwinska, Agata; Marquis, Melanie; Godin, Mathilde; Marchand, Laetitia; Sinquin, Corinne; Garnier, Catherine; Jonchere, Camille; Chedeville, Claire; Le Visage, Catherine; Guicheux, Jerome; Colliec-jouault, Sylvia; Cuenot, Stephane.
Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating...
Tipo: Text
Palavras-chave: Exopolysaccharide; Growth factor; Microparticles; Microfluidics; Bioactivity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00477/58882/61432.pdf

Enzymes de dépolymérisation d'exopolysaccharides bactériens marins
Provedor de dados: 5
Autores: Lelchat, Florian.
Exopolysaccharides (EPSs) are a class of biopolymer synthesized by Eukarya, Archea and Procarya.Bacterial EPSs are involved in biofilm establishment and biofouling phenomenon. These polymers have physicochemical and biological properties suitable with biotechnological valorization. At the opposite, their involvment in biofouling of pathogenic strains can be problematic.Enzymatic depolymerization process are necessary for EPSs structural elucidation, Bioactive oligosaccharides production or to disrupt polysaccharidic biofilms. The highlight of enzymatic phenomenon can help to understand biogeochimical process in the ocean. Nevertheless the important structural diversity as well as their complexity make the sourcing of specific enzymes difficult.Two...
Tipo: Text
Palavras-chave: Bactéries marines; Bactériophages marins; Phages marins; Exopolysaccharide; Marine bacteria; Marine bacteriophages; Marine phages; Polysaccharidase; Cazyme.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00498/60944/64337.pdf

Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus
Provedor de dados: 5
Autores: Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine.
Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the...
Tipo: Text
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf

Transparent Exopolymeric Particles (TEP) Selectively Increase Biogenic Silica Dissolution From Fossil Diatoms as Compared to Fresh Diatoms
Provedor de dados: 5
Autores: Toullec, Jordan; Moriceau, Brivaela.
Diatom production is mainly supported by the dissolution of biogenic silica (bSiO(2)) within the first 200 m of the water column. The upper oceanic layer is enriched in dissolved and/or colloidal organic matter, such as exopolymeric polysaccharides (EPS) and transparent exopolymeric particles (TEP) excreted by phytoplankton in large amounts, especially at the end of a bloom. In this study we explored for the first time the direct influence of TEP-enriched diatom excretions on bSiO(2) dissolution. Twelve dissolution experiments on fresh and fossil diatom frustules were carried out on seawater containing different concentrations of TEP extracted from diatom cultures. Fresh diatom frustules were cleaned from the organic matter by low ash temperature, and...
Tipo: Text
Palavras-chave: Exopolysaccharide; Diatom excretion; Diatomite; Chaetoceros muelleri; Biogenic silica; BSiO(2) dissolution; TEP.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00616/72795/72270.pdf

Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp nov., from New Caledonia
Provedor de dados: 5
Autores: Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir, H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle.
Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size...
Tipo: Text
Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf

Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment
Provedor de dados: 5
Autores: Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf

Deep-sea Hydrothermal Vent Bacteria as a Source of Glycosaminoglycan-Mimetic Exopolysaccharides
Provedor de dados: 5
Autores: Zykwinska, Agata; Marchand, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Bacteria have developed a unique strategy to survive in extreme environmental conditions through the synthesis of an extracellular polymeric matrix conferring upon the cells a protective microenvironment. The main structural component of this complex network constitutes high-molecular weight hydrophilic macromolecules, namely exopolysaccharides (EPS). EPS composition with the presence of particular chemical features may closely be related to the specific conditions in which bacteria evolve. Deep-sea hydrothermal vent bacteria have already been shown to produce EPS rich in hexosamines and uronic acids, frequently bearing some sulfate groups. Such a particular composition ensures interesting functional properties, including biological activities mimicking...
Tipo: Text
Palavras-chave: Exopolysaccharide; Glycosaminoglycan; Uronic acid; Hexosamine; Phylogenetic analysis.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00506/61756/65728.pdf

Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae
Provedor de dados: 5
Autores: Lebellenger, Lou; Verrez-bagnis, Veronique; Passerini, Delphine; Delbarre Ladrat, Christine.
Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis...
Tipo: Text
Palavras-chave: Vibrionaceae; Exopolysaccharide; Genetic biodiversity; Biosynthesis; Vibrio diabolicus; HE800 EPS.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00424/53536/54392.pdf

Use of Natural Antimicrobial Peptides and Bacterial Biopolymers for Cultured Pearl Production
Provedor de dados: 5
Autores: Simon-colin, Christelle; Gueguen, Yannick; Bachere, Evelyne; Kouzayha, Achraf; Saulnier, Denis; Gayet, Nicolas; Guezennec, Jean.
Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with...
Tipo: Text
Palavras-chave: Pearl oyster; Exopolysaccharide; Tachyplesin; Antimicrobial.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00275/38599/37128.pdf

Exopolysaccharides produced by bacteria isolated from deep-sea hydrothermal vents: new agents with therapeutic potential
Provedor de dados: 5
Autores: Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean.
Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new...
Tipo: Text
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf

Modifications d'un exopolysaccharide biosynthétisé par une bactérie issue des écosystèmes Hydrothermaux profonds
Provedor de dados: 5
Autores: Petit, Anne-celine.
Le travail présenté dans ce mémoire concerne la modification d'un exopolysaccharide (EPS), l'HYD 657, biosynthétisé par une bactérie issue des écosystèmes hydrothermaux profonds. Ce polysaccharide d'un poids moléculaire de 1,8.106 Da est composé de 40-50% d'oses neutres et de 30- 40% d'acides uroniques. L'hypothèse de structure la plus probable décrit son unité de répétition comme un undécasaccharide possédant 3 ramifications courtes. Cet EPS, sous sa forme native, a déjà fait l'objet d'une étude approfondie en vue d'applications dans le domaine de la cosmétique pour ses propriétés apaisantes en application topique cutanée.  Pour élargir le spectre d'applications de cet EPS, la réduction de sa masse molaire a été envisagée.  La première partie décrit la...
Tipo: Text
Palavras-chave: Oxidation; Sulfation; Esterification; Metallic catalysts; Sonochemistry; Deep sea hydrothermal; Depolymerization; Exopolysaccharide; Oxydation; Sulfatation; Estérification; Catalyse métallique; Ultrasons; Source hydrothermale; Dépolymérisation; Exopolysaccharide.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-1232.pdf

Lactobacillus fermentum TDS030603株の生産する中性多糖の構造研究
Provedor de dados: 19
Autores: Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6...
Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183

Effects of carbohydrate source on physicochemical properties of the exopolysaccharide produced by Lactobacillus fermentum TDS030603 in a chemically defined medium
Provedor de dados: 19
Autores: Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly...
Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694

Screening of basidiomycetes for the production of exopolysaccharide and biomass in submerged culture
Provedor de dados: 59
Autores: Maziero,Rosana; Cavazzoni,Valeria; Bononi,Vera Lúcia Ramos.
Fifty-six strains of Basidiomycetes, including native Brazilian fungi isolated from different ecosystems and edible mushrooms, were screened for production of exopolysaccharides and biomass in submerged culture. Agaricus sp. (CCB 280) and Oudemansiella canarii (Jungh.) Hohn (CCB 179) were the highest exopolysaccharide producers (6.01 and 3.54 g dry w./l respectively) after 7 days of incubation. The best producer of biomass was Schizophyllum commune Fr.:Fr. (CCB 473) with 16.68 g dry w./l in 14 days of incubation. When the culture filtrate was submitted to freezing prior to polysaccharide precipitation, a gelatinous fraction was formed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Basidiomycetes; Exopolysaccharide; Biomass; Submerged culture.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100015

Effect of carbon source on production, characterization and bioactivity of exopolysaccharide produced by Phellinus vaninii Ljup
Provedor de dados: 42
Autores: XU,CHUNPING; YU,JINWEI; ZHAO,SHANSHAN; WU,SHUNGSHUNG; HE,PEIXIN; JIA,XUEWEI; LIU,YANQI; MAO,DUOBIN.
ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phellinus vaninii Ljup; Exopolysaccharide; Carbon source; Antioxidant activity; Composition.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502033

Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain
Provedor de dados: 42
Autores: Paulo,Elinalva M.; Boffo,Elisangela F.; Branco,Alexsandro; Valente,Ângela M.M.P.; Melo,Itamar S.; Ferreira,Antonio G.; Roque,Milton R.A.; Assis,Sandra A. de.
The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leuconostoc pseudomesenteroides; Exopolysaccharide; Polymer; Dextran.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018

Influence of gastrointestinal system conditions on adhesion of exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to caco-2 cells
Provedor de dados: 52
Autores: Darilmaz,Derya Onal; Aslım,Belma; Suludere,Zekiye; Akca,Gülcin.
This study aimed to assess the transit tolerance of potential probiotic dairy Lactobacillus strains in human uppergastrointestinal tract in vitro, and to evaluate the effect of EPS production on the viability and adhesion of these strains. Survival and adhesion of two exopolysaccharide (EPS)-producing L. delbrueckii subsp. bulgaricus strains (B3 and B2) and E. coli ATCC11229 were assessed after the exposure of different pH (gastric juice) and gastric plus pancreatic juice challenges. In the artificial gastric juice (pH 2), both the viability of the strain B3 and B2 was decreased. Artificial juice treatments significantly reduced the adhesion to caco-2 cells (P&lt; 0.05). High EPS-producing B3 survived better in the adverse gastrointestinal conditions and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adhesion; Exopolysaccharide; Caco-2 Cells; Gastric-pancreatic juices; Lactobacillus delbrueckii subsp. bulgaricus.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500009

Nutritional requirements of Zymomonas mobilis CCT 4494 for levan production
Provedor de dados: 52
Autores: Ernandes,Fernanda Maria Pagane Guereschi; Garcia-Cruz,Crispin Humberto.
The aim of this work was to study the nutritional requirements of Zymomonas mobilis CCT 4494 for levan production in three chemically defined media. During the optimization of the fermentative process for the production of the exopolysaccharide, different concentrations of glucose, fructose and sucrose as carbon source and yeast extract as vitamin source were tested. Variations of incubation temperature and initial pH of the medium were observed. The results showed that medium containing 20.0% sucrose and 0.5% yeast extract, with initial pH of 7.0, incubated at 30°C gave a 43.0% yield of the biopolymer.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zymomonas mobilis; Levan; Exopolysaccharide; Production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300021

Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
Provedor de dados: 58
Autores: Sun,Xinyi; Hao,Long; Ma,Hua; Li,Tong; Zheng,Lan; Ma,Zhao; Zhai,Guoyin; Wang,Liqin; Gao,Shanglong; Liu,Xiaonan; Jia,Mengshi; Jia,Le.
The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pleurotus eryngii SI-02; Exopolysaccharide; Extraction; Antioxidant activity; In vitro.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009

Bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggregation under salt stress
Provedor de dados: 58
Autores: Qurashi,Aisha Waheed; Sabri,Anjum Nasim.
To compensate for stress imposed by salinity, biofilm formation and exopolysaccharide production are significant strategies of salt tolerant bacteria to assist metabolism. We hypothesized that two previously isolated salt-tolerant strains Halomonas variabilis (HT1) and Planococcus rifietoensis (RT4) have an ability to improve plant growth, These strains can form biofilm and accumulate exopolysacharides at increasing salt stress. These results showed that bacteria might be involved in developing microbial communities under salt stress and helpful in colonizing of bacterial strains to plant roots and soil particles. Eventually, it can add to the plant growth and soil structure. We investigated the comparative effect of exopolysacharide and biofilm formation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Cicer arietinum; Exopolysaccharide; Salinity; Soil aggregates.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300046

Industrial wastewater as raw material for exopolysaccharide production by Rhizobium leguminosarum
Provedor de dados: 58
Autores: Sellami,Mohamed; Oszako,Tomasz; Miled,Nabil; Ben Rebah,Faouzi.
The objective of this study was to evaluate the exopolysaccharide (EPS) production by Rhizobium leguminosarum cultivated in wastewater generated by oil companies (WWOC1 and WWOC2) and fish processing industry (WWFP). The results obtained in Erlenmeyer flasks indicated that the rhizobial strain grew well in industrial wastewater. Generally, wastewater composition affected the growth and the EPS production. WWFP allowed good bacterial growth similar to that obtained with the standard medium (YMB). During growth, various quantities of EPS were produced and yields varied depending on the media. Growing in YMB, EPS production did not exceed 9.7 g/L obtained after 72 h of growth. In wastewater, the maximum EPS value reached 11.1 g/L obtained with the fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Industrial wastewater; Biopolymer; Exopolysaccharide; Rhizobium.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200407

Gordonia polyisoprenivorans from groundwater contaminated with landfill leachate in a subtropical area: characterization of the isolate and exopolysaccharide production
Provedor de dados: 58
Autores: Fusconi,Roberta; Godinho,Mirna Januária Leal; Hernández,Isara Lourdes Cruz; Bossolan,Nelma Regina Segnini.
A strain of Gordonia sp. (strain Lc), from landfill leachate-contaminated groundwater was characterized by polyphasic taxonomy and studied for exopolysaccharide (EPS) production. The cells were Gram-positive, catalase-positive, oxidase-negative and non-motile. The organism grew both aerobically and, in anoxic environment, in the presence of NaNO3. Rods occured singly, in pairs or in a typical coryneform V-shaped. The organism had morphological, physiological and chemical properties consistent with its assignment to the genus Gordonia and mycolic and fatty acid pattern that corresponded to those of G. polyisoprenivorans DSM 44302T. The comparison of the sequence of the first 500 bases of the 16S rDNA of strain Lc gave 100% similarity with the type strain of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gordonia polyisoprenivorans; Exopolysaccharide; Groundwater; Landfill.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200014

Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust
Provedor de dados: 58
Autores: Mikiashvili,Nona A; Isikhuemhen,Omoanghe S; Ohimain,Elijah I.
Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grifola frondosa; Exopolysaccharide; Laccase; Lignin degradation; Peroxidase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300031

Growth and exopolysaccharide production by Streptococcus thermophilus ST1 in skim milk
Provedor de dados: 58
Autores: Zhang,Tiehua; Zhang,Chunhong; Li,Shengyu; Zhang,Yanchun; Yang,Zhennai.
To analyze the exopolysaccharide (EPS) production by Streptococcus thermophilus ST1, cultures were cultivated in 10% (w/v) reconstituted skim milk under different growth conditions including various temperatures and pHs of growth medium, supplementation of the medium with various carbon sources (glucose, lactose, sucrose, galactose and fructose) and nitrogen source (whey protein concentrate, or WPC). The results showed that most EPS production by strain ST1 was obtained at a temperature (42°C) and pH (6.5) optimal for its growth. Supplementation of the skim milk medium with either carbohydrates or WPC increased both growth and polymer formation by different extents, with sucrose being most effective among the carbon sources tested. Under the optimal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exopolysaccharide; Streptococcus thermophilus ST1; Skim milk.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400033