Consulta: subjectFacets:"Extracellular matrix"
Registros recuperados: 48
Data/hora: 07/06/2024 00:23:19

Epistasis of polymorphisms related to the articular cartilage extracellular matrix in knee osteoarthritis: Analysis-based multifactor dimensionality reduction
Provedor de dados: 74
Autores: Fernández-Torres,Javier; Martínez-Nava,Gabriela Angélica; Zamudio-Cuevas,Yessica; Lozada,Carlos; Garrido-Rodríguez,Daniela; Martínez-Flores,Karina.
Abstract Osteoarthritis (OA) is a complex disease with a multifactorial etiology. The genetic component is one of the main associated factors, resulting from interactions between genes and environmental factors. The aim of this study was to identify gene-gene interactions (epistasis) of the articular cartilage extracellular matrix (ECM) in knee OA. Ninety-two knee OA patients and 147 healthy individuals were included. Participants were genotyped in order to evaluate nine variants of eight genes associated with ECM metabolism using the OpenArray technology. Epistasis was analyzed using the multifactor dimensionality reduction (MDR) method. The MDR analysis showed significant gene-gene interactions between MMP3 (rs679620) and COL3A1 (rs1800255), and between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epistasis; Extracellular matrix; Knee osteoarthritis; Multifactor dimensionality reduction; Polymorphisms.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400102

Elastin (ELN) gene point mutation in patients with inguinal hernia
Provedor de dados: 74
Autores: Rodrigues,Consuelo Junqueira; Yoo,Jin Hwan; Rodrigues Junior,Aldo Junqueira.
Groin hernias emerge at the myopectineal orifice of Fruchaud which is closed off by the fascia transversalis. Our previous studies showed structural and quantitative changes of the fascia transversalis elastic fibers of inguinal hernia patients and elderly people. The present study used single-strand conformation polymorphism (SSCP) elastin (analysis to investigate the 34 exons of the ELN gene of 49 inguinal hernia patients (7 females, 42 males aged 58.7 ± 19.82 years) and 75 non-herniated controls (35 females, 40 males aged 46.2 ± 14.32 years). We found that 47 patients and 24 controls had an abnormal exon 20 pattern caused by a g28197A &gt; G missense mutation leading to an S422G amino acid substitution in the elastin hydrophobic domain. The g28197A &gt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fascia transversalis; Extracellular matrix; Elastic fibers; Elastin gene; Inguinal hernia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100009

Species-specific differences in diatom-induced anomalies in calanoid copepod embryogenesis: are they linked to the stock-piled antioxidants?
Provedor de dados: 13
Autores: Khanaychenko, A. N.
Egg production and viability of co-occurring in nature copepods, Calanus helgolandicus and Calanoides carinatus, fed diatom, Thalassiosira rotula, and then switched to dinoflagellate Prorocentrum minimum, were compared in experimental conditions. Egg production of both species on diatom diet was similar (up to 17-22 eggs.female-1.d-1) but no viable nauplii were observed. Development was arrested at different stages depending on degree of anomalies. Abnormal embryos displayed various degrees of deteriorations in pigment distribution and organization of extracellular matrix. Reproductive responses of C.carinatus both to negative (diatom) and positive (dinoflagellate) diets were postponed in comparison with quick responses of C.helgolandicus. After 3 days of...
Tipo: Journal Contribution
Palavras-chave: Calanoid copepods; Diatom; Embryo; Anomaly; Extracellular matrix; Pigment; Каланоидные копеподы; Диатомовые; Эмбрион; Аномалии; Экстраклеточный матрикс; Пигмент Diatoms Embryos Pigments Pigments Diatoms http://aims.fao.org/aos/agrovoc/c_5875 http://aims.fao.org/aos/agrovoc/c_32657.
Ano: 2004
URL: http://repository.ibss.org.ua/dspace/handle/99011/92

Comparative histology in the liver and spleen of three species of freshwater teleost
Provedor de dados: 83
Autores: Sales,Camila F.; Silva,Regianne F.; Amaral,Marília G. C.; Domingos,Fabrício F. T.; Ribeiro,Rosy I. M. A.; Thomé,Ralph G.; Santos,Hélio B.
ABSTRACT We describe and compare the histology of liver and spleen ofGeophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) and Hoplias aff. malabaricus (Characiformes), tropical freshwater fishes. InG. brasiliensisandH. aff. malabaricusthe hepatocytes were arranged in tubular form whereas in H. franciscithey cord-like. In all species, hepatocytes presented glycogen, but in G. brasiliensis and H. aff. malabaricus they showed strong stained for hemossiderin in the cytoplasm. InG. brasiliensis and H. aff. malabaricus, melanomacrophage centres (MMCs) were associated to hepatic structures and only in G. brasiliensis was observed intrahepatic exocrine pancreas. The spleen, in all species, was characterized by red and white pulp without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Hepatocytes; Intrahepatic exocrine pancreas; Melanomacrophage centres; Red and White pulp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100209

Wave onset in central gray matter - its intrinsic optical signal and phase transitions in extracellular polymers
Provedor de dados: 42
Autores: FERNANDES-DE-LIMA,VERA M.; KOGLER,JOÃO E.; BENNATON,JOCELYN; HANKE,WOLFGANG.
The brain is an excitable media in which excitation waves propagate at several scales of time and space. ''One-dimensional'' action potentials (millisecond scale) along the axon membrane, and spreading depression waves (seconds to minutes) at the three dimensions of the gray matter neuropil (complex of interacting membranes) are examples of excitation waves. In the retina, excitation waves have a prominent intrinsic optical signal (IOS). This optical signal is created by light scatter and has different components at the red and blue end of the spectrum. We could observe the wave onset in the retina, and measure the optical changes at the critical transition from quiescence to propagating wave. The results demonstrated the presence of fluctuations preceding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intrinsic optic signals; Volume phase transitions; Polymers; Spreading depression; Central gray matter; Extracellular matrix.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000300006

Modulators of axonal growth and guidance at the brain midline with special reference to glial heparan sulfate proteoglycans
Provedor de dados: 42
Autores: CAVALCANTE,LENY A.; GARCIA-ABREU,JOSÉ; MOURA NETO,VIVALDO; SILVA,LUIZ CLAUDIO; WEISSMÜLLER,GILBERTO.
Bilaterally symmetric organisms need to exchange information between the left and right sides of their bodies to integrate sensory input and to coordinate motor control. Thus, an important choice point for developing axons is the Central Nervous System (CNS) midline. Crossing of this choice point is influenced by highly conserved, soluble or membrane-bound molecules such as the L1 subfamily, laminin, netrins, slits, semaphorins, Eph-receptors and ephrins, etc. Furthermore, there is much circumstantial evidence for a role of proteoglycans (PGs) or their glycosaminoglycan (GAG) moieties on axonal growth and guidance, most of which was derived from simplified models. A model of intermediate complexity is that of cocultures of young neurons and astroglial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astrocytes; Axon growth; Extracellular matrix; Heparan sulfate; Midline; Midbrain.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400010

Heparan sulfate proteoglycans: structure, protein interactions and cell signaling
Provedor de dados: 42
Autores: Dreyfuss,Juliana L.; Regatieri,Caio V.; Jarrouge,Thais R.; Cavalheiro,Renan P.; Sampaio,Lucia O.; Nader,Helena B.
Heparan sulfate proteoglycans are ubiquitously found at the cell surface and extracellular matrix in all the animal species. This review will focus on the structural characteristics of the heparan sulfate proteoglycans related to protein interactions leading to cell signaling. The heparan sulfate chains due to their vast structural diversity are able to bind and interact with a wide variety of proteins, such as growth factors, chemokines, morphogens, extracellular matrix components, enzymes, among others. There is a specificity directing the interactions of heparan sulfates and target proteins, regarding both the fine structure of the polysaccharide chain as well precise protein motifs. Heparan sulfates play a role in cellular signaling either as receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans and protein interactions; Growth factors; Focal adhesion; Extracellular matrix; Cell cycle; Cell proliferation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300007

Interleukin-1&amp;#946; regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model
Provedor de dados: 47
Autores: Fontana,Vanina Andrea; Sanchez,Melisa; Cebral,Elisa; Calvo,Juan Carlos.
Implantation is one of the most regulated processes in human reproduction, by endocrine and immunological systems. Cytokines are involved in embryo-maternal communication and an impaired balance could result in pregnancy loss. Here we investigated the effect of interleukin 1-&amp;#946; on the activity of two important metalloproteinases (MMP-2 and MMP-9) that are involved in extracellular matrix remodeling as well as the secretion of leptin, one of the reproductive hormones actively regulating their activity and secretion. We found that IL-1&amp;#946; activates matrix metalloproteinase activity as well as increases leptin secretion. We propose that this interleukin, through the regulation of leptin, in turn activates matrix metalloproteinases which results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokines; Embryo implantation; Placenta; Extracellular matrix.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100005

Red Wine administration to Apolipoprotein E-deficient Mice reduces their Macrophage-derived Extracellular Matrix Atherogenic Properties
Provedor de dados: 48
Autores: KAPLAN,MARIELLE; AVIRAM,MICHAEL.
Proteoglycans (PGs) from the arterial extracellular matrix (ECM) contribute to the trapping of LDL and oxidized LDL (Ox-LDL) in the arterial wall, a phenomenon called "lipoprotein retention". Moreover, we have shown that subsequent to their binding to the matrix, LDL and Ox-LDL are taken up by macrophages. Oxidative stress significantly increases macrophage secretion of ECM-PGs, lipoprotein binding to the ECM and the uptake of ECM-retained lipoproteins by macrophages. The aim of the present study was to determine whether red wine administration to atherosclerotic mice would affect their peritoneal macrophage-derived extracellular matrix properties, such as the glycosaminoglycan content and the ability to bind LDL. In addition, we questioned the ability of...
Tipo: Journal article
Palavras-chave: Antioxidants; Atherosclerosis; Extracellular matrix; Macrophages; Polyphenols; Proteoglycans; Red wine.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200010

Integrin-&amp;#946;1, not integrin-&amp;#946;5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Provedor de dados: 48
Autores: Zeng,Qiangcheng; Guo,Yong; Liu,Yongming; Li,Ruixin; Zhang,Xinchang; Liu,Lu; Wang,Yang; Zhang,Xizheng; Zou,Xianqiong.
BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-&amp;#946; (&amp;#946;1, &amp;#946;5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-&amp;#946; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-&amp;#946;1 siRNA or integrin-&amp;#946;5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µ&amp;#949;) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted...
Tipo: Journal article
Palavras-chave: Tensile strain; Osteoblast; Extracellular matrix; Integrin; SiRNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025

Composition and significance of glycosaminoglycans in the uterus and placenta of mammals
Provedor de dados: 52
Autores: Oliveira,Gleidson Benevides de; Vale,André Menezes do; Santos,Amílton Cesar dos; Moura,Carlos Eduardo Bezerra de; Rocha,Hugo Alexandre de Oliveira; Oliveira,Moacir Franco de.
Glycosaminoglycans (GAGs) are heteropolysaccharides in mammalian tissue and consist of repeated disaccharide units with mono-sulfated or non-sulfated monosaccharides. GAGs are important components of the Extracellular Matrix (ECM) with several physiological roles, in the recognition, migration, adhesion, proliferation and differentiation processes. They are also important in angiogenesis, blood homeostasis, immune reactions, follicule development and also in the development of pathologies such as infertility, tumors and metastases. It has been shown that the profile of glycosaminoglycans in the uterine and placental tissues is highly variable throughout the reproductive cycle and during pregnancy. It may be directly related to their physiological or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Hormonal fluctuation; Reproductive events; Euterium mammals; Pathology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400512

Decellularized Bovine Articular Cartilage Matrix Reinforced by Carboxylated-SWCNT for Tissue Engineering Application
Provedor de dados: 52
Autores: Mohammadie,Zari Majidi; Parivar,Kazem; Shahri,Nasser Mahdavi; Fereidoni,Masoud; Hayati-Roodbari,Nasim.
ABSTRACT Nanotubes with their unique properties have diversified mechanical and biological applications. Due to similarity of dimensions with extracellular matrix (ECM) elements, these materials are used in designing scaffolds. In this research, Carboxylated Single-Wall Carbon Nanotubes in optimization of decellularized scaffold of bovine articular cartilage was used. At first, the articular cartilage was decellularized. Then the scaffolds were analyzed in: (i) decellularized scaffolds, and (ii) scaffolds plunged into homogenous suspension of nanotubes in distilled water, were smeared with Carboxylated-SWCNT. The tissue rings derived from the rabbit's ear were assembled with reinforced scaffolds and they were placed in a culture media for 15 days. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Articular cartilage; Carboxylated-SWCNT; Extracellular matrix; Natural scaffold; Tissue engineering.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100607

Histochemical study of fibrillar proteins of the extracellular matrix in benign and malignant mammary neoplasms in dogs
Provedor de dados: 62
Autores: Martins,Ana Maria Cristina Rabello Pinto da Fonseca; Tamaso,Elia; Guerra,José Luiz.
The aim of the present study was to study some of the proteins that form the extracellular matrix of 54 benign and malignant mammary neoplasms in dogs, using histochemical methods: Picrosirius and polarization microscopy for collagenous fibers, Gordon -Sweats's method for reticular fibers and Weigert's fucsine-resorcine method for elastic fibers. A large variability in quantity, distribution and characteristics of the matrix components was observed in the different types of neoplasms. Collagen type I, III and elements of the elastic system had different distribution in benign and malignant neoplasms. The simple Picrossirius method and under polarization enabled visualization of collagen as thick fibers irregularly distributed in the stroma of carcinomas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mammary neoplasms; Collagen; Dogs; Extracellular matrix.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100008

Retrospective review and systematic study of mammary tumors in dogs and characteristics of the extracellular matrix
Provedor de dados: 62
Autores: Martins,Ana Maria Cristina Rabello Pinto da Fonseca; Tamaso,Elia; Guerra,José Luiz.
The aim of the present study was to perform a retrospective review, from 1932 to 1999, in order to establish the number of cases of mammary tumors in dogs in the records at the Pathology Department of Faculdade de Medicina Veterinária - USP, as well as the presence of desmoplasia and cartilaginous and bone metaplasia in 578 of these tumors. Among the 537 malignant tumors, 13.05% were simple tubular adenocarcinomas, 3.91% were complex tubular adenocarcinomas, 7.26% simple papillary adenocarcinomas, 4.28% complex papillary adenocarcinomas , 23.27% simple papillary cystadenocarcinomas ,8.37% complex papillary cystadenocarcinomas,16.38% simple solid adenocarcinomas ,6.70% complex solid adenocarcinomas, 2.04% simple spindle cell carcinomas, 1.11% simple spindle...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mammary neoplasms; Dogs; Pathology; Extracellular matrix; Frequency.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100007

The conveyor belt hypothesis for thymocyte migration: participation of adhesion and de-adhesion molecules
Provedor de dados: 56
Autores: Villa-Verde,D.M.S.; Calado,T.C.; Ocampo,J.S.P.; Silva-Monteiro,E.; Savino,W.
Thymocyte differentiation is the process by which bone marrow-derived precursors enter the thymus, proliferate, rearrange the genes and express the corresponding T cell receptors, and undergo positive and/or negative selection, ultimately yielding mature T cells that will represent the so-called T cell repertoire. This process occurs in the context of cell migration, whose cellular and molecular basis is still poorly understood. Kinetic studies favor the idea that these cells leave the organ in an ordered pattern, as if they were moving on a conveyor belt. We have recently proposed that extracellular matrix glycoproteins, such as fibronectin, laminin and type IV collagen, among others, produced by non-lymphoid cells both in the cortex and in the medulla,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymus; Thymic nurse cells; Adhesion molecules; Extracellular matrix; Galectin-3.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500010

Hypotrophy of conduit artery walls of the offspring of nitric oxide-defective rats
Provedor de dados: 56
Autores: Kristek,F.; Gerová,M.
The objective of the present study was to investigate the structure of the arterial walls of the offspring stemming from nitric oxide (NO)-defective hypertensive parents. The parents were treated with N G-nitro-L-arginine methyl ester (40 mg kg-1 day-1) for 5 weeks. Blood pressure was measured noninvasively in six 30-day-old rats and nine age-matched controls. The cardiovascular system was perfused with glutaraldehyde at 120 mmHg. The thoracic aorta and carotid artery were processed for electron microscopy, and geometry was determined by light microscopy. Endothelial cells, smooth muscle cells (SMC) and extracellular matrix (ECM) were determined by the point counting method in electron micrographs of the carotid artery. The blood pressure of experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artery; Nitric oxide; Offspring; Hypertension; Vascular smooth muscle; Extracellular matrix; Morphometry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400018

Dimensions and dynamics in integrin function
Provedor de dados: 56
Autores: Yamada,K.M.; Pankov,R.; Cukierman,E.
Integrins play crucial roles in cell adhesion, migration, and signaling by providing transmembrane links between the extracellular matrix and the cytoskeleton. Integrins cluster in macromolecular complexes to generate cell-matrix adhesions such as focal adhesions. In this mini-review, we compare certain integrin-based biological responses and signaling during cell interactions with standard 2D cell culture versus 3D matrices. Besides responding to the composition of the matrix, cells sense and react to physical properties that include three-dimensionality and rigidity. In routine cell culture, fibroblasts and mesenchymal cells appear to use focal adhesions as anchors. They then use intracellular actomyosin contractility and dynamic, directional integrin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Cell adhesion; Integrin; Fibronectin; Signal transduction; Migration.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800001

Expression of extracellular matrix components and their receptors in the central nervous system during experimental Toxoplasma gondii and Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,A.A.; Roffê,E.; Lannes-Vieira,J.
Alterations in extracellular matrix (ECM) expression in the central nervous system (CNS) usually associated with inflammatory lesions have been described in several pathological situations including neuroblastoma and demyelinating diseases. The participation of fibronectin (FN) and its receptor, the VLA-4 molecule, in the migration of inflammatory cells into the CNS has been proposed. In Trypanosoma cruzi infection encephalitis occurs during the acute phase, whereas in Toxoplasma infection encephalitis is a chronic persisting process. In immunocompromised individuals such as AIDS patients, T. cruzi or T. gondii infection can lead to severe CNS damage. At the moment, there are no data available regarding the molecules involved in the entrance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Central nervous system; Extracellular matrix.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500013

Transforming growth factor beta activity in urine of patients with type 2 diabetes and diabetic nephropathy
Provedor de dados: 56
Autores: Rivarola,E.W.R.; Moyses-Neto,M.; Dantas,M.; da-Silva,C.G.; Volpini,R.; Coimbra,T.M.
Diabetic nephropathy (DN) is characterized structurally by progressive mesangial deposition of extracellular matrix (ECM). Transforming growth factor-ß (TGF-ß) is considered to be one of the major cytokines involved in the regulation of ECM synthesis and degradation. Several studies suggest that an increase in urinary TGF-ß levels may reflect an enhanced production of this polypeptide by the kidney cells. We evaluated TGF-ß in occasional urine samples from 14 normal individuals and 23 patients with type 2 diabetes (13 with persistent proteinuria &amp;gt;500 mg/24 h, DN, 6 with microalbuminuria, DMMA, and 4 with normal urinary albumin excretion, DMN) by enzyme immunoassay. An increase in the rate of urinary TGF-ß excretion (pg/mg UCreat.) was observed in...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: TGF-ß urinary excretion; Type 2 diabetes; Proteinuria; Diabetic nephropathy; Extracellular matrix.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200011

Effect of photodynamic therapy on the extracellular matrix and associated components
Provedor de dados: 56
Autores: Pazos,M.d.C.; Nader,H.B.
In many countries, photodynamic therapy (PDT) has been recognized as a standard treatment for malignant conditions (for example, esophageal and lung cancers) and non-malignant ones such as age-related macular degeneration and actinic keratoses. The administration of a non-toxic photosensitizer, its selective retention in highly proliferating cells and the later activation of this molecule by light to form reactive oxygen species that cause cell death is the principle of PDT. Three important mechanisms are responsible for the PDT effectiveness: a) direct tumor cell kill; b) damage of the tumor vasculature; c) post-treatment immunological response associated with the leukocyte stimulation and release of many inflammatory mediators like cytokines, growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photodynamic therapy; Extracellular matrix; Adhesion; Chemical mediators; Matrix metalloproteinases.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800002

The extracellular matrix provides directional cues for neuronal migration during cerebellar development
Provedor de dados: 56
Autores: Porcionatto,M.A.
Normal central nervous system development relies on accurate intrinsic cellular programs as well as on extrinsic informative cues provided by extracellular molecules. Migration of neuronal progenitors from defined proliferative zones to their final location is a key event during embryonic and postnatal development. Extracellular matrix components play important roles in these processes, and interactions between neurons and extracellular matrix are fundamental for the normal development of the central nervous system. Guidance cues are provided by extracellular factors that orient neuronal migration. During cerebellar development, the extracellular matrix molecules laminin and fibronectin give support to neuronal precursor migration, while other molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Central nervous system; Cerebellum; Neuron; Migration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300001

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix
Provedor de dados: 56
Autores: Paez-Pereda,M.; Kuchenbauer,F.; Arzt,E.; Stalla,G.K.
The extracellular matrix is a three-dimensional network of proteins, glycosaminoglycans and other macromolecules. It has a structural support function as well as a role in cell adhesion, migration, proliferation, differentiation, and survival. The extracellular matrix conveys signals through membrane receptors called integrins and plays an important role in pituitary physiology and tumorigenesis. There is a differential expression of extracellular matrix components and integrins during the pituitary development in the embryo and during tumorigenesis in the adult. Different extracellular matrix components regulate adrenocorticotropin at the level of the proopiomelanocortin gene transcription. The extracellular matrix also controls the proliferation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Integrins; Pituitary gland; Pituitary adenomas; Pituitary hormones; Cell proliferation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000005

Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Provedor de dados: 56
Autores: Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004

A comparative study of aging of the elastic fiber system of the diaphragm and the rectus abdominis muscles in rats
Provedor de dados: 56
Autores: Rodrigues,C.J.; Rodrigues Junior,A.J.
In the present study the age-related changes of the striated muscle elastic fiber system were investigated in the diaphragm and rectus abdominis muscles of 1-, 4-, 8- and 18-month-old rats. The activation patterns of these muscles differ in that the diaphragm is regularly mobilized tens of times every minute during the entire life of the animal whereas the rectus abdominis, although mobilized in respiration, is much less and more irregularly activated. The elastic fibers were stained by the Verhoeff technique for mature elastic fibers. Weigert stain was used to stain mature and elaunin elastic fibers, and Weigert-oxone to stain mature, elaunin and oxytalan elastic fibers. The density of mature and elaunin elastic fibers showed a progressive increase with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Elastin; Extracellular matrix; Diaphragm; Rectus abdominis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200008

CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell
Provedor de dados: 56
Autores: Aguiar,D.P.; Coelho-Aguiar,J.M.; Abreu,J.G.
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300004

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers
Provedor de dados: 56
Autores: Lenzi,H.L.; Kimmel,E.; Schechtman,H.; Pelajo-Machado,M.; Vale,B.S.; Panasco,M.S.; Lenzi,J.A.
The collagen structure of isolated and in situ liver granuloma from Swiss Webster mice infected with Schistosoma mansoni was sequentially and three-dimensionally analyzed during different times of infection (early acute, acute, transitional acute-chronic, and chronic phases) by laser scanning confocal microscopy and electron scanning variable vacuum microscopy. The initial granuloma structure is characterized by vascular collagen residues and by anchorage points (or fiber radiation centers), from where collagenous fibers are angularly shed and self-assembled. During the exudative-productive stage, the self-assembly of these fibers minimizes energy and mass through continuous tension and focal compression. The curvature or angles between collagen fibers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Granuloma; Schistosoma mansoni; Collagen; Extracellular matrix; Confocal microscopy.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500018

Extracellular matrix molecules as targets for brown spider venom toxins
Provedor de dados: 56
Autores: Veiga,S.S.; Zanetti,V.C.; Braz,A.; Mangili,O.C.; Gremski,W.
Loxoscelism, the term used to describe lesions and clinical manifestations induced by brown spider's venom (Loxosceles genus), has attracted much attention over the last years. Brown spider bites have been reported to cause a local and acute inflammatory reaction that may evolve to dermonecrosis (a hallmark of envenomation) and hemorrhage at the bite site, besides systemic manifestations such as thrombocytopenia, disseminated intravascular coagulation, hemolysis, and renal failure. The molecular mechanisms by which Loxosceles venoms induce injury are currently under investigation. In this review, we focused on the latest reports describing the biological and physiopathological aspects of loxoscelism, with reference mainly to the proteases recently...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brown spider; Venom; Extracellular matrix; Proteolytic effect.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700002

Thymocyte migration: an affair of multiple cellular interactions?
Provedor de dados: 56
Autores: Savino,W.; Ayres Martins,S.; Neves-dos-Santos,S.; Smaniotto,S.; Ocampo,J.S.P.; Mendes-da-Cruz,D.A.; Terra-Granado,E.; Kusmenok,O.; Villa-Verde,D.M.S.
Cell migration is a crucial event in the general process of thymocyte differentiation. The cellular interactions involved in the control of this migration are beginning to be defined. At least chemokines and extracellular matrix proteins appear to be part of the game. Cells of the thymic microenvironment produce these two groups of molecules, whereas developing thymocytes express the corresponding receptors. Moreover, although chemokines and extracellular matrix can drive thymocyte migration per se, a combined role for these molecules appears to contribute to the resulting migration patterns of thymocytes in their various stages of differentiation. The dynamics of chemokine and extracellular matrix production and degradation is not yet well understood....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thymocyte migration; Extracellular matrix; Integrins; Chemokines; Thymic epithelial cells; Thymic nurse cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800007

Interactions of laminin with the amyloid ß peptide: Implications for Alzheimer's disease
Provedor de dados: 56
Autores: Morgan,C.; Inestrosa,N.C.
Extensive neuronal cell loss is observed in Alzheimer's disease. Laminin immunoreactivity colocalizes with senile plaques, the characteristic extracellular histopathological lesions of Alzheimer brain, which consist of the amyloid ß (Aß) peptide polymerized into amyloid fibrils. These lesions have neurotoxic effects and have been proposed to be a main cause of neurodegeneration. In order to understand the pathological significance of the interaction between laminin and amyloid, we investigated the effect of laminin on amyloid structure and toxicity. We found that laminin interacts with the Aß1-40 peptide, blocking fibril formation and even inducing depolymerization of preformed fibrils. Protofilaments known to be intermediate species of Aß fibril formation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Laminin; Amyloid ß peptide; Neuronal injury; Neurodegenerative disease.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500006

Understanding the mechanisms of lung mechanical stress
Provedor de dados: 56
Autores: Garcia,C.S.N.B.; Prota,L.F.M.; Morales,M.M.; Romero,P.V.; Zin,W.A.; Rocco,P.R.M.
Physical forces affect both the function and phenotype of cells in the lung. Bronchial, alveolar, and other parenchymal cells, as well as fibroblasts and macrophages, are normally subjected to a variety of passive and active mechanical forces associated with lung inflation and vascular perfusion as a result of the dynamic nature of lung function. These forces include changes in stress (force per unit area) or strain (any forced change in length in relation to the initial length) and shear stress (the stress component parallel to a given surface). The responses of cells to mechanical forces are the result of the cell's ability to sense and transduce these stimuli into intracellular signaling pathways able to communicate the information to its interior. This...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mechanical forces; Mechanosensors; Mechanotransduction; Cell-cell interactions; Cytokines; Extracellular matrix.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600001

Modulation of fibronectin expression in the central nervous system of Lewis rats with experimental autoimmune encephalomyelitis
Provedor de dados: 56
Autores: De-Carvalho,M.C.A.; Chimelli,L.M.C.; Quirico-Santos,T.
Fibronectin (FN), a large family of plasma and extracellular matrix (ECM) glycoproteins, plays an important role in leukocyte migration. In normal central nervous system (CNS), a fine and delicate mesh of FN is virtually restricted to the basal membrane of cerebral blood vessels and to the glial limitans externa. Experimental autoimmune encephalomyelitis (EAE), an inflammatory CNS demyelinating disease, was induced in Lewis rats with a spinal cord homogenate. During the preclinical phase and the onset of the disease, marked immunolabelling was observed on the endothelial luminal surface and basal lamina of spinal cord and brainstem microvasculature. In the paralytic phase, a discrete labelling was evident in blood vessels of spinal cord and brainstem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental autoimmune encephalomyelitis (EAE); Extracellular matrix; Fibronectin; Central nervous system.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500012

Is pancreas development abnormal in the non-obese diabetic mouse, a spontaneous model of type I diabetes?
Provedor de dados: 56
Autores: Homo-Delarche,F.
Despite extensive genetic and immunological research, the complex etiology and pathogenesis of type I diabetes remains unresolved. During the last few years, our attention has been focused on factors such as abnormalities of islet function and/or microenvironment, that could interact with immune partners in the spontaneous model of the disease, the non-obese diabetic (NOD) mouse. Intriguingly, the first anomalies that we noted in NOD mice, compared to control strains, are already present at birth and consist of 1) higher numbers of paradoxically hyperactive ß cells, assessed by in situ preproinsulin II expression; 2) high percentages of immature islets, representing islet neogenesis related to neonatal ß-cell hyperactivity and suggestive of in utero ß-cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NOD mice; Postnatal pancreas development; Antigen-presenting cell; Extracellular matrix.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400002

Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor
Provedor de dados: 56
Autores: Song,Rui; Bian,Hui-Ning; Lai,Wen; Chen,Hua-De; Zhao,Ke-Seng.
Basic fibroblast growth factor (bFGF) regulates skin wound healing; however, the underlying mechanism remains to be defined. In the present study, we determined the effects of bFGF on the regulation of cell growth as well as collagen and fibronectin expression in fibroblasts from normal human skin and from hypertrophic scars. We then explored the involvement of mitochondria in mediating bFGF-inducedeffects on the fibroblasts. We isolated and cultivated normal and hypertrophic scar fibroblasts from tissue biopsies of patients who underwent plastic surgery for repairing hypertrophic scars. The fibroblasts were then treated with different concentrations of bFGF (ranging from 0.1 to 1000 ng/mL). The growth of hypertrophic scar fibroblasts became slower with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BFGF; Hypertrophic scar; Fibroblast; Extracellular matrix; Mitochondria; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500004

Transient outward potassium current and Ca2+ homeostasis in the heart: beyond the action potential
Provedor de dados: 56
Autores: Bassani,R.A.
Normal central nervous system development relies on accurate intrinsic cellular programs as well as on extrinsic informative cues provided by extracellular molecules. Migration of neuronal progenitors from defined proliferative zones to their final location is a key event during embryonic and postnatal development. Extracellular matrix components play important roles in these processes, and interactions between neurons and extracellular matrix are fundamental for the normal development of the central nervous system. Guidance cues are provided by extracellular factors that orient neuronal migration. During cerebellar development, the extracellular matrix molecules laminin and fibronectin give support to neuronal precursor migration, while other molecules...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Central nervous system; Cerebellum; Neuron; Migration.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300010

A connection between extracellular matrix and hormonal signals during the development of the human fetal adrenal gland
Provedor de dados: 56
Autores: Chamoux,E.; Otis,M.; Gallo-Payet,N.
The human adrenal cortex, involved in adaptive responses to stress, body homeostasis and secondary sexual characters, emerges from a tightly regulated development of a zone-specific secretion pattern during fetal life. Its development during fetal life is critical for the well being of pregnancy, the initiation of delivery, and even for an adequate adaptation to extra-uterine life. As early as from the sixth week of pregnancy, the fetal adrenal gland is characterized by a highly proliferative zone at the periphery, a concentric migration accompanied by cell differentiation (cortisol secretion) and apoptosis in the central androgen-secreting fetal zone. After birth, a strong reorganization occurs in the adrenal gland so that it better fulfills the newborn's...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal gland; Development; Human fetus; Extracellular matrix; Angiotensin II; Adrenocorticotropin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000006

Building bone tissue: matrices and scaffolds in physiology and biotechnology
Provedor de dados: 56
Autores: Riminucci,M.; Bianco,P.
Deposition of bone in physiology involves timed secretion, deposition and removal of a complex array of extracellular matrix proteins which appear in a defined temporal and spatial sequence. Mineralization itself plays a role in dictating and spatially orienting the deposition of matrix. Many aspects of the physiological process are recapitulated in systems of autologous or xenogeneic transplantation of osteogenic precursor cells developed for tissue engineering or modeling. For example, deposition of bone sialoprotein, a member of the small integrin-binding ligand, N-linked glycoprotein family, represents the first step of bone formation in ectopic transplantation systems in vivo. The use of mineralized scaffolds for guiding bone tissue engineering has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Bone matrix; Bone formation; Biomaterials; Tissue engineering.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800008

Cellular and matrix interactions during the development of T lymphocytes
Provedor de dados: 56
Autores: Owen,J.J.T.; McLoughlin,D.E.; Suniara,R.K.; Jenkinson,E.J.
The thymus contains an extensive extracellular matrix. Although thymocytes express integrins capable of binding to matrix molecules, the functional significance of the matrix for T cell development is uncertain. We have shown that the matrix is associated with thymic fibroblasts which are required for the CD44+ CD25+ stage of double negative (CD4-8-) thymocyte development. The survival of cells at this stage is dependent on IL-7 and we propose that the role of fibroblasts is to present, via the matrix, IL-7 to developing T cells.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Epithelial cells; Fibroblasts; Integrins; Interleukin-7; Thymus.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500008

Decellularization of placentas: establishing a protocol
Provedor de dados: 56
Autores: Leonel,L.C.P.C.; Miranda,C.M.F.C.; Coelho,T.M.; Ferreira,G.A.S.; Caãada,R.R.; Miglino,M.A.; Lobo,S.E.
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas. Canine placentas were subjected to ten preliminary tests to analyze the efficacy of parameters such as the type of detergents, freezing temperatures and perfusion. Two protocols were chosen for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Placenta; Decellularization; Acellular biomaterial.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100601

Role of gelatinases MMP-2 and MMP-9 in tissue remodeling following acute lung injury
Provedor de dados: 56
Autores: Corbel,M.; Boichot,E.; Lagente,V.
Acute lung injury is characterized by a severe disruption of alveolo-capillary structures and includes a variety of changes in lung cell populations. Evidence suggests the occurrence of rupture of the basement membranes and interstitial matrix remodeling during acute lung injury. The dynamic equilibrium of the extracellular matrix (ECM) under physiological conditions is a consequence of the balance between the regulation of synthesis and degradation of ECM components. Matrix metalloproteinases (MMPs) represent a group of enzymes involved in the degradation of most of the components of the ECM and therefore participate in tissue remodeling associated with pathological situations such as acute lung injury. MMP activity is regulated by proteolytic activation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix metalloproteinase; TIMPs; Acute lung injury; ARDS; LPS; Extracellular matrix.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700004

Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation
Provedor de dados: 56
Autores: Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V.
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007

Trypanosoma cruzi infection: a continuous invader-host cell cross talk with participation of extracellular matrix and adhesion and chemoattractant molecules
Provedor de dados: 56
Autores: Marino,A.P.M.P.; Silva,A.A.; Pinho,R.T.; Lannes-Vieira,J.
Several lines of evidence have shown that Trypanosoma cruzi interacts with host extracellular matrix (ECM) components producing breakdown products that play an important role in parasite mobilization and infectivity. Parasite-released antigens also modulate ECM expression that could participate in cell-cell and/or cell-parasite interactions. Increased expression of ECM components has been described in the cardiac tissue of chronic chagasic patients and diverse target tissues including heart, thymus, central nervous system and skeletal muscle of experimentally T. cruzi-infected mice. ECM components may adsorb parasite antigens and cytokines that could contribute to the establishment and perpetuation of inflammation. Furthermore, T. cruzi-infected mammalian...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Extracellular matrix; Cell adhesion; Cell migration; Adhesion molecules; Chemokines.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800020

Manipulation of a quasi-natural cell block for high-efficiency transplantation of adherent somatic cells
Provedor de dados: 56
Autores: Chung,H.J.; Hassan,M.M.; Park,J.O.; Kim,H.J.; Hong,S.T.
Recent advances have raised hope that transplantation of adherent somatic cells could provide dramatic new therapies for various diseases. However, current methods for transplanting adherent somatic cells are not efficient enough for therapeutic applications. Here, we report the development of a novel method to generate quasi-natural cell blocks for high-efficiency transplantation of adherent somatic cells. The blocks were created by providing a unique environment in which cultured cells generated their own extracellular matrix. Initially, stromal cells isolated from mice were expanded in vitro in liquid cell culture medium followed by transferring the cells into a hydrogel shell. After incubation for 1 day with mechanical agitation, the encapsulated cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quasi-natural cell block; Transplantation; Scaffold; Extracellular matrix; Hydrogel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500392

Importance of hyaluronan biosynthesis and degradation in cell differentiation and tumor formation
Provedor de dados: 56
Autores: Heldin,P.
Hyaluronan is an important connective tissue glycosaminoglycan. Elevated hyaluronan biosynthesis is a common feature during tissue remodeling under both physiological and pathological conditions. Through its interactions with hyaladherins, hyaluronan affects several cellular functions such as cell migration and differentiation. The activities of hyaluronan-synthesizing and -degrading enzymes have been shown to be regulated in response to growth factors. During tumor progression hyaluronan stimulates tumor cell growth and invasiveness. Thus, elucidation of the molecular mechanisms which regulate the activities of hyaluronan-synthesizing and -degrading enzymes during tumor progression is highly desired.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hyaluronan; Synthase; Hyaluronidase; Tumor invasion; Extracellular matrix; Pericellular matrix.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800002

Microencapsulation and tissue engineering as an alternative treatment of diabetes
Provedor de dados: 56
Autores: Maria-Engler,S.S.; Mares-Guia,M.; Correa,M.L.C.; Oliveira,E.M.C.; Aita,C.A.M.; Krogh,K.; Genzini,T.; Miranda,M.P.; Ribeiro,M.; Vilela,L.; Noronha,I.L.; Eliaschewitz,F.G.; Sogayar,M.C.
In the 70's, pancreatic islet transplantation arose as an attractive alternative to restore normoglycemia; however, the scarcity of donors and difficulties with allotransplants, even under immunosuppressive treatment, greatly hampered the use of this alternative. Several materials and devices have been developed to circumvent the problem of islet rejection by the recipient, but, so far, none has proved to be totally effective. A major barrier to transpose is the highly organized islet architecture and its physical and chemical setting in the pancreatic parenchyma. In order to tackle this problem, we assembled a multidisciplinary team that has been working towards setting up the Human Pancreatic Islets Unit at the Chemistry Institute of the University of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human pancreatic islets; Pancreas; Extracellular matrix; Stem cells; Islet microencapsulation; Transplantation; Ductal cells.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600001

Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma
Provedor de dados: 56
Autores: Yu,Cheng Hao; Zhao,Jin Shuang; Zhao,Hui; Peng,Teng; Shen,Dong Cheng; Xu,Qiu Xia; Li,Yao; Webb,R. Clinton; Wang,Mong Heng; Shi,Xing Ming; Peng,Cheng; Ding,Wei Jun.
The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E2) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uterine leiomyoma; Transcriptional profiling; Extracellular matrix; Curcumae rhizoma; Sparganii rhizoma.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600606

The extracellular matrix in multiple sclerosis: an update
Provedor de dados: 56
Autores: Sobel,R.A.
Extracellular matrix (ECM) molecules play important roles in the pathobiology of the major human central nervous system (CNS) inflammatory/demyelinating disease multiple sclerosis (MS). This mini-review highlights some recent work on CNS endothelial cell interactions with vascular basement membrane ECM as part of the cellular immune response, and roles for white matter ECM molecules in demyelination and remyelination in MS lesions. Recent basic and clinical investigations of MS emphasize axonal injury, not only in chronic MS plaques, but also in acute lesions; progressive axonal degeneration in normal-appearing white matter also may contribute to brain and spinal cord atrophy in MS patients. Remodeling of the interstitial white matter ECM molecules that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Axon regeneration; Central nervous system; Extracellular matrix; Proteoglycans; Multiple sclerosis; Versican.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500007

Regulatory roles of microtubule-associated proteins in neuronal morphogenesis. Involvement of the extracellular matrix
Provedor de dados: 56
Autores: Ramírez,G.; Alvarez,A.; Garcia-Abreu,J.; Gomes,F.C.A.; Moura-Neto,V.; Maccioni,R.B.
As a result of recent investigations, the cytoskeleton can be viewed as a cytoplasmic system of interconnected filaments with three major integrative levels: self-assembling macromolecules, filamentous polymers, e.g., microtubules, intermediate filaments and actin filaments, and supramolecular structures formed by bundles of these filaments or networks resulting from cross-bridges between these major cytoskeletal polymers. The organization of this biological structure appears to be sensitive to fine spatially and temporally dependent regulatory signals. In differentiating neurons, regulation of cytoskeleton organization is particularly relevant, and the microtubule-associated protein (MAP) tau appears to play roles in the extension of large neuritic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Tau functions; Regulatory patterns; Neuronal morphogenesis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500015

Elastin increases biofilm and extracellular matrix production of Aspergillus fumigatus
Provedor de dados: 58
Autores: Brandão,Ildnay de Souza Lima; Oliveira-Moraes,Heloiza Maria da Silva; Motta,Cristina Maria de Souza; Oliveira,Neiva Tinti de; Magalhães,Oliane Maria Correia.
Abstract Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10 mg/mL of elastin, a significant increase (absorbance p &lt; 0.0001; dry weight p &lt; 0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8 mg (dry weight) of biofilm in 72 h. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus fumigatus; Biofilm; Elastin; Extracellular matrix; Hydrophobicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300675