Consulta: subjectFacets:"Extracellular products"
Registros recuperados: 5
Data/hora: 01/06/2024 08:57:59

Caractérisation de la virulence d'une souche de Vibrio aestuarianus, pathogène de l'huître Crassostrea gigas
Provedor de dados: 5
Autores: Labreuche, Yannick.
In France. shellfish production is a traditional. well-established industry. mainly based upon commercial farming of the Pacifie oyster. Crassostrea gigas. Since the 1980's, annual mass mortalities of C. gigas have been reported during summer. Several studies on this subject have demonstrated that these mortality outbreaks resulted from complex interactions between the physiologieal andJor genetic status of the oysters. environmental factors, and one or more infectious agents, among which are bacteria of the genus Vibrio.
Tipo: Text
Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarlanus; Pathogénicité; Produits extracellulaires; Métalloprotéase; Hémocytes; Interactions hôte/pathogène; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Pathogenicity; Extracellular products; Metalloprotease; Hemocytes; Host/pathogen interactions.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00034/14561/11859.pdf

Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses
Provedor de dados: 5
Autores: Labreuche, Yannick; Le Roux, Frederique; Henry, Joel; Zatylny, Celine; Huvet, Arnaud; Lambert, Christophe; Soudant, Philippe; Mazel, Didier; Nicolas, Jean-louis.
Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP...
Tipo: Text
Palavras-chave: Vibrio aestuarianus; Metalloprotease; Crassostrea gigas; Oyster; Hemocytes; Extracellular products.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00015/12649/9588.pdf

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone
Provedor de dados: 5
Autores: Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf

Toxic factors of Vibrio strains pathogenic to shrimp
Provedor de dados: 5
Autores: Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C; Martin, B.
Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida...
Tipo: Text
Palavras-chave: Vibrio; Vibrio penaeicida; Shrimp; Extracellular products; Temperature.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00000/10543/7600.pdf

Vibrio tubiashii en France: description d'isolats pathogènes affectant des mollusques et étude de leurs mécanismes de virulence
Provedor de dados: 5
Autores: Mersni Achour, Rachida.
The oyster farming constitutes one of the major components of the global aquaculture. However, this sector is facing abnormal mortalities outbreaks that affect diploid and triploid oyster at their different life stages, in the hatcheries and in the field. During bivalve molluscs mortality events in France, bacteria initially classified into Harveyi group, were regularly isolated along with herpes virus, V. splendidus or V. aestuarianus. In order to fine tune the taxonomic affiliation of those isolates, a genotypic and phenotypic approach was used. The bacterial isolates, initially misclassified into the Harveyi clade, were shown to be genetically closed to V. tubiashii strains already recognized as the main causative agents of larvae and juvenile mollusc...
Tipo: Text
Palavras-chave: Crassostrea gigas; Isolats français; Vibrio tubiashii; Hémocytes; Pathogénicité; Infection expérimentale; Produits extracellulaires; Facteurs de virulence; Metalloprotéases; Crassostrea gigas; French isolates; Vibrio tubiashii; Hemocytes; Pathogenicity; Experimental infection; Extracellular products.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00230/34093/32540.pdf