Consulta: subjectFacets:"Flow cytometry"
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Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae).
Provedor de dados: 32
Autores: Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/569

Evolución genética de cuatro especies de Anastrepha spp. (Díptera: Tephritidae).
Provedor de dados: 32
Autores: Teodoro Pardo, Claudia Verónica de.
El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los...
Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/569

Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Provedor de dados: 69
Autores: Mendoza,Héctor; de la Jara,Adelina; Freijanes,Karen; Carmona,Laura; Ramos,Ana Alexandra; de Sousa Duarte,Vanessa; Serafim Varela,João Carlos.
In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye...
Tipo: Journal article
Palavras-chave: Carotene; Carotenogenesis; Dunaliella salina; Flow cytometry; Nile red.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400005

Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis
Provedor de dados: 69
Autores: Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M.
The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant....
Tipo: Journal article
Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010

A new variety of Bacopa monnieri obtained by in vitro polyploidization
Provedor de dados: 69
Autores: Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol.
The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences...
Tipo: Journal article
Palavras-chave: Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002

Genetic diversity in a world germplasm collection of tall fescue
Provedor de dados: 74
Autores: Cuyeu,Romina; Rosso,Beatriz; Pagano,Elba; Soto,Gabriela; Fox,Romina; Ayub,Nicolás Daniel.
Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR markers; Genetic diversity; Flow cytometry; Tall fescue; Genetic resources.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200014

Nuclear DNA content and karyotype of Rosewood (Aniba rosaeodora)
Provedor de dados: 74
Autores: Contim,Luis Antônio Serrão; Carvalho,Carlos Roberto de; Martins,Franciele Alline; Freitas,Danival Vieira de.
Rosewood (Aniba rosaeodora Ducke, Lauraceae) is ecologically and economically important to the Amazon region. As a consequence of its economic importance, rosewood populations have been decimated in the Amazon forest. Species of nine genera of the Lauraceae family have characterized karyotypes with n = x = 12 chromosomes in the gametophytic phase but the genus Aniba is one of the least studied Lauraceae genera with a previously undescribed genome. We used cytogenetic techniques to determine that the A. rosaeodora karyotype contained 12 pairs (2n = 24) of relatively small submetacentric chromosomes with lengths ranging from 1.34 to 2.25 mum and a nucleolar organizer region (NOR) in the short arm of chromosome 7. Flow cytometry gave 2C = 2.32 pg of DNA,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosewood; Aniba rosaeodora; Lauraceae; Karyotype; Flow cytometry.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500017

Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae)
Provedor de dados: 74
Autores: Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019

Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa
Provedor de dados: 74
Autores: Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J.
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016

Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum
Provedor de dados: 74
Autores: Monteiro,Rita; Carvalho,Cláudia; Collares-Pereira,Maria João.
This study aimed to define the karyotype of the recently described Iberian endemic Iberochondrostoma almacai, to revisit the previously documented chromosome polymorphisms of its sister species I. lusitanicum using C-, Ag-/CMA3 and RE-banding, and to compare the two species genome sizes. A 2n = 50 karyotype (with the exception of a triploid I. lusitanicum specimen) and a corresponding haploid chromosome formula of 7M:15SM:3A (FN = 94) were found. Multiple NORs were observed in both species (in two submetacentric chromosome pairs, one of them clearly homologous) and a higher intra and interpopulational variability was evidenced in I. lusitanicum. Flow cytometry measurements of nuclear DNA content showed some significant differences in genome size both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag/CMA3-banding; Flow cytometry; NORs; Chromosome polymorphism; Spontaneous triploidy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200011

Spatial and temporal variability of the pico- and nanophytoplankton and bacterioplankton in a deep Polynesian atoll lagoon
Provedor de dados: 5
Autores: Thomas, Y.; Garen, Pierre; Courties, C.; Charpy, L.
Spatial and temporal scales of variability in phyto- and bacterioplankton abundance were investigated in a deep atoll lagoon (Ahe, French Polynesia), Ahe Lagoon showed a classical phytoplankton composition, but its picophytoplankton (Prochlorococcus, Synechococcus and picoeukaryotes) concentrations are higher than the mean for Polynesian atoll lagoons. Phyto- and bacterioplankton descriptors were highly variable, with mean CVs of &gt;20% in most cases: day night, day-to-day, vertical, lagoonal and seasonal. Phytoplankton biomass, which was estimated by in vivo fluorescence, was higher in the deep layer than in the upper layer. This pattern was detected on a day night scale, showing transitory vertical gradients, and through long-term events that were...
Tipo: Text
Palavras-chave: Flow cytometry; Picophytoplankton; Nanophytoplankton; Bacterioplankton; Vertical distribution; Day-to-day dynamics; Large-scale heterogeneity; Deep atoll lagoon; French Polynesia.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00003/11425/7963.pdf

Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis.
Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced...
Tipo: Text
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Quiniou, Louis; Beker, Beatriz; Haberkorn, Hansy; Marie, Dominique; De La Broise, Denis.
The impacts of the fungicide Opus(A (R)) (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 mu g l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 mu g l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant...
Tipo: Text
Palavras-chave: Microcosm; Phytoplankton community; Epoxiconazole; TTGE; Flow cytometry; HPLC.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00088/19888/17547.pdf

Seasonal to hour variation scales in abundance and production of total and particle-attached bacteria in the open NW Mediterranean Sea (0-1000 m)
Provedor de dados: 5
Autores: Mevel, G.; Vernet, M.; Goutx, M.; Ghiglione, J. F.
We present the vertical and temporal dynamics of total vs. particle-attached bacterial abundance and activity over a 5 week period under summer to autumn transition in NW Mediterranean Sea. At a weekly time scale, total bacterial biomass and production in the euphotic layers was significantly correlated with phytoplanktonic biomass. At an hourly time scale, total bacterial biomass responded very rapidly to chlorophyll a fluctuations, suggesting a tight coupling between phytoplankton and bacteria for resource partitioning during the summer-autumn transition. In contrast, no influence of diel changes on bacterial parameters was detected. Episodic events such as coastal water intrusions had a significant positive effect on total bacterial abundance and...
Tipo: Text
Palavras-chave: Nucleic acid content; Free living bacteria; Top down control; Flow cytometry; Atlantic ocean; Water column; Spring bloom; Bottom up; North sea; Biogeochemical signifiance.
Ano: 2008
URL: https://archimer.ifremer.fr/doc/00208/31973/30408.pdf

Pollutant effects on Pacific oyster, Crassostrea gigas (Thunberg), hemocytes: Screening of 23 molecules using flow cytometry
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan.
The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed...
Tipo: Text
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf

A study of autophagy in hemocytes of the Pacific oyster,   Crassostrea gigas
Provedor de dados: 5
Autores: Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf

Short-term interactions between phytoplankton and intertidal seagrass vegetation in a coastal lagoon (Bassin d'Arcachon, SW France)
Provedor de dados: 5
Autores: De Wit, Rutger; Troussellier, Marc; Courties, Claude; Buffan-dubau, Evelyne; Lemaire, Emmanuelle.
We experimentally simulated the temporality of the interactions between intertidal seagrasses and phytoplankton in the context of nutrient enrichment from continental origin. Phytoplankton development was retarded after 1-day exposure to intertidal Zostera noltii Hornemann vegetation samples, with respect to control treatments. This was not explained by resource competition and we hypothesized a direct interference between seagrass leaves and phytoplankton. After separation from the vegetation in 5-day incubations, the final yield of large phytoplankton, mainly diatoms and haptophytes, was determined by nutrient loading. In contrast, Synechococcus-like cells (SYN), phototrophic picoeukaryotes and nanophytoplankton decreased after day 3, most likely due to...
Tipo: Text
Palavras-chave: Competition; Allelopathy; Zostera noltii; Sediment; Intertidal vegetation; Flow cytometry; Photosynthetic pigments.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00106/21717/19414.pdf

Diploid and triploid Pacific oysters, Crassostrea gigas (Thunberg), reared at two heights above sediment in Marennes-Oleron Basin, France: Difference in mortality, sexual maturation and hemocyte parameters
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Soletchnik, Patrick; Madec, Patrick; Geairon, Philippe; Le Moine, Olivier; Renault, Tristan.
Summer mortality is an important economic concern for the Pacific oyster, Crassostrea gigas, industry all over the world and particularly in France. Outbreaks appear when oysters are in gonadal maturation. Bivalve defence mechanisms against pathogen invasion are assumed by circulating cells, the hemocytes. A field experiment was conducted in the Marennes-Oleron Basin (Charente-Maritime, France) in order to monitor hemocyte parameters, gametogenesis and mortality rates among C. gigas during 7 months from March to September 2002. Diploid (from natural bed and from hatchery) and triploid oysters were placed at 15 cm or 70 cm above sediment at Marennes-Oleron Basin. Triploid animals were included because they are considered as sterile animals. Mortality rates...
Tipo: Text
Palavras-chave: Sexual maturation; Summer mortality; Triploids; Diploids; Flow cytometry; Hemocyte activities; Crassostrea gigas; Pacific oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2923.pdf

Spatial and temporal extension of eutrophication associated with shrimp farm wastewater discharges in the New Caledonia lagoon
Provedor de dados: 5
Autores: Thomas, Yoann; Courties, Claude; El Helwe, Yasmin; Herbland, Alain; Lemonnier, Hugues.
Shrimp farming in New Caledonia typically uses a flow-through system with water exchange rates as a tool to maintain optimum hydrological and biological parameters for the crop. Moreover, the effluent shows hydrobiological characteristics (minerals, phytoplankton biomass and organic matter) significantly higher than that of the receiving environment. Separate surveys were carried out in a bay (CH Bay) with a medium-size intensive farm (30 ha) (PO) and in a mangrove-lined creek (TE Creek) near a larger semi-intensive farm (133 ha) (SO). Net loads of nitrogen exported from the semi-intensive farm and the intensive farm amounted to 0.68 and 1.36 kg ha(-1) day(-1), respectively. At CH Bay, discharge effects were spatially limited and clearly restricted to...
Tipo: Text
Palavras-chave: Environmental impact; Shrimp farming; Eutrophication; Flow cytometry; New Caledonia.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00014/12527/9882.pdf

Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites
Provedor de dados: 5
Autores: Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois.
High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes).   Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered...
Tipo: Text
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf

Effets d'un mélange de pesticides sur les hémocytes d'huître creuse, Crassostrea gigas.
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Geret, Florence; Haure, Joel; Martin, Jean-louis; Burgeot, Thierry; Thomas-guyon, Hélène; Renault, Tristan.
Shellfish industry is often realised in estuarine areas, subjected to pollutions due to agricultural activities. The harmful effects of pesticides on animals inhabiting these estuarine zones are poorly documented. Bivalve molluscs represent an interesting model because they are sedentary and they filter water intensively. Effects of pesticides on haemocytes functions were tested in Pacific oyster, Crassostrea gigas and analysed by flow cytometry. A combination of pesticide at environmental concentrations was tested in vivo. Animals used corresponded to two genetically selected populations produced in hatchery.
Tipo: Text
Palavras-chave: Haemocytes; Flow cytometry; Pesticides; Crassostrea gigas.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-924.pdf

TOPHYPAC. Tolérance des communautés phytoplanctoniques aux phytosanitaires dans le panache de la Charente. Programme Évaluation et réduction des risques liés à l’utilisation des Pesticides
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Guesdon, Stephane; Bechemin, Christian; Chiffoleau, Jean-francois; Brach-papa, Christophe; Soudant, Philippe; Beker, Beatriz; Jadas-hécart, Alain.
TOPHYPAC is a field study that was conducted in the Charente river estuary (Marennes-Oléron bay). The main goal was to assess the possible impact of chemical contamination with copper and pesticides on phytoplankton communities. Physical, chemical (nutrients, metals and pesticides) and biological (phytoplankton community) parameters were monitored in the field during three years, from 2011 to 2014. In 2012 and 2013, field sampling was coupled with experimental exposure of phytoplankton communities to copper and to an herbicide cocktail, after transportation to the lab: the effects were assessed using photosynthesis endpoint and cellular densities. TOPHYPAC allowed to set the framework of Charente estuary contamination with pesticides: herbicides glyphosate...
Tipo: Text
Palavras-chave: Continuum eau douce/eau côtière; Pesticides; Métaux; Communautés phytoplanctoniques; Efficacité de photosynthèse; PICT; Tolérance; Cytométrie en flux; Toxicité chronique; Freshwater/coastal water continuum; Pesticides; Metals; Phytoplankton communities; Photosynthesis efficiency; PICT; Tolerance; Flow cytometry; Chronic toxicity.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00300/41115/40289.pdf

In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas Guyon, H; Renault, Tristan.
In the past decades. shellfish culture has developed in a significant way around the world. However, culture areas are Often Subject to recurring anthropic pollution. The recrudescent presence of industrial wastes is a source of heavy metals and results ill pollutant transfer towards the aquatic environment in estuarine areas. Because of their mode of life. bivalves. including Mussels and oysters. are suggested as ideal indicator organisms. The development of techniques allowing the analysis of the effects of Pollutants on bivalve biology may lead to the monitoring of pollutant transfer in estuarine areas. in this context. the effects of cadmium and mercury On defence mechanisms were analysed in Pacific oysters. Crassostrea gigas. Pollutant effects were...
Tipo: Text
Palavras-chave: Toxicity; Cellular activities; Phenoloxidase; Mercury; Cadmium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-691.pdf

Study of atrazine effects on Pacific oyster, Crassostrea gigas, haemocytes
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Bouilly, Karine; Lapegue, Sylvie; Thomas-guyon, H.
Shellfish farming is an important economic activity around the world. This activity often takes place in areas subjected to various recurring pollutions. The recrudescent use of herbicides in agriculture including atrazine implies pollutant transfer towards aquatic environment in estuarine areas. Harmful effects of such substances on animals in marine environment, particularly on cultured bivalves, are poorly documented. Bivalve molluscs such as mussels and oysters have been postulated as ideal indicator organisms because of their way of life. They filter large volumes of seawater and may therefore accumulate and concentrate eontaJ.11inants within their tissues. Moreover, development of techniques allowing effect analysis of such compounds on bivalve...
Tipo: Text
Palavras-chave: Crassostrea gigas; Haemocytes; Flow cytometry; Atrazine; Cellular activities; Toxicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-2827.pdf

Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text
Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf

Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea
Provedor de dados: 5
Autores: Da Costa, Fiz; Le Grand, Fabienne; Quere, Claudie; Bougaran, Gael; Cadoret, Jean-paul; Robert, Rene; Soudant, Philippe.
Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates...
Tipo: Text
Palavras-chave: Tisochrysis lutea; Nitrogen limitation; Growth phase; Flow cytometry; Fatty acids; Lipid classes.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00399/51074/51936.pdf

Métabolisme énergétique du spermatozoïde d'huître creuse (Crassostrea gigas)
Provedor de dados: 5
Autores: Boulais, Myrina.
The present study aims at describing the energy metabolism of Pacific oyster, Crassostrea gigas, spermatozoon. The main objectives are: (1) describing the kinetic of cellular and biochemical characteristics of Pacific oyster spermatozoon during its movement phase, (2) identifying the metabolic pathways to produce energy and their substrates, (3) determining the involvement of energy metabolism in fertilization success. The results showed the long duration (ranging 24 to 72h) of the movement of Pacific oyster spermatozoon and the maintenance of a high intracellular ATP content until the end of the movement phase. The role of oxidative phosphorylation (OXPHOS) in ATP production was highlighted. Alternative ATP producing pathways might contribute to sustain...
Tipo: Text
Palavras-chave: Fécondation; Sperme; Analyse du mouvement; Métabolisme énergétique; RMN; Cytométrie en flux; Crassostrea gigas; Reproduction; Fertilization; Sperm; Movement analysis; Energetic metabolism; NMR; Flow cytometry.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00498/60940/64334.pdf

Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan.
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf

Phytoplankton dynamics in the southern New Caledonian lagoon during a southeast trade winds event
Provedor de dados: 5
Autores: Neveux, Jacques; Lefebvre, Jean-pierre; Le Gendre, Romain; Dupouy, Cecile; Gallois, Francis; Courties, Claude; Gerard, Philippe; Fernandez, Jean-michel; Ouillon, Sylvain.
During the Echolag cruise (13 February-8 March 2007), the effect of 16-30 knot trade winds on abundance, community structure and dynamics of phytoplankton was investigated in the southern part of the New Caledonian coral reefs and surrounding oceanic waters. In this area, the coral reefs form two horn-like structures (hereinafter referred to as 'horn reefs'), oriented south-east, and separated by a relatively deep valley. Three repeated samplings at one week intervals of a 32 station array showed that trade winds induce surface water cooling and significant enrichment of mid- and bottom waters above the reef shelf. This enrichment appeared as the result of a general rise of the oceanic thermocline and nitracline due to the combination of trade winds and...
Tipo: Text
Palavras-chave: Chlorophyll; Phycoerythrin; Flow cytometry; Phytoplankton; In vivo fluorescence; Growth rate; Trade winds; Lagoon; Internal wave; New Caledonia.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00013/12437/9258.pdf

Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie.
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant...
Tipo: Text
Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf

In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes
Provedor de dados: 5
Autores: Lambert, Christophe; Soudant, Philippe; Jegaden, Marine; Delaporte, Maryse; Labreuche, Yannick; Moal, Jeanne; Samain, Jean-francois.
Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types...
Tipo: Text
Palavras-chave: NO synthase; NADPH oxidase; Flow cytometry; Reactive nitrogen intermediate RNI; Reactive oxygen intermediate ROI; Hemocytes; Crassostrea gigas.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3057.pdf

Isolation and primary culture of gill and digestive gland cells from the common mussel Mytilus edulis
Provedor de dados: 5
Autores: Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry.
As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater...
Tipo: Text
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf

Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Becker, Beatriz; Marie, Dominique; Haberkorn, Hansy; Coroller, Louis; De La Broise, Denis.
The effects of the herbicide Roundup (R) (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6 m depth, and 10% of their water content was changed every other day. The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 165 and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1 mu g L-1 (ANOSIM,p=0.055; R=0.53), and 10 mu g L-1 (ANOSIM,...
Tipo: Text
Palavras-chave: Microcosm; Marine microbial communities; Glyphosate; TTGE; Flow cytometry.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00088/19887/17546.pdf

Eutrophication in a tropical pond: Understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses
Provedor de dados: 5
Autores: Lucas, Ronan; Courties, C.; Herbland, Alain; Goulletquer, Philippe; Marteau, Anne-laure; Lemonnier, Hugues.
In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4...
Tipo: Text
Palavras-chave: Tropical shrimp aquaculture; Ecosystem shift; Phytoplankton; Bacterioplankton; Vibrio; Flow cytometry.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00029/14068/11312.pdf

Seasonal variation of stable isotope ratios of size-fractionated zooplankton in the Bay of Marseille (NW Mediterranean Sea)
Provedor de dados: 5
Autores: Banaru, Daniela; Carlotti, Francois; Barani, Aude; Gregori, Gerald; Neffati, Nada; Harmelin-vivien, Mireille.
Stable isotope ratios of six size fractions of zooplankton (80 to &gt;2000 mm) were analyzed seasonally in 2009-2010 at the SOMLIT site in the Bay of Marseille. Isotopic ratios generally increased with zooplankton size. The highest delta N-15 values were observed in the 1000-2000 mm fraction. The largest size class (&gt;2000 mu m), dominated by gelatinous plankton, had lower delta N-15 values due to the low isotopic signatures of most of these organisms. In the larger size fractions (&gt;1000 mu m), isotopic ratios were measured at the taxon level. Brachyuran, stomatopod, teleost and cephalopod larvae showed the highest delta N-15 values, and salps and pteropods the lowest ones. Lower values of both delta C-13 and delta N-15 were recorded in winter and...
Tipo: Text
Palavras-chave: Plankton; Delta C-13; Delta N-15; Size classes; Flow cytometry.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00372/48275/48536.pdf

Survey for neoplasia in Macoma balthica from the Gulf of Gdansk by flow cytometry
Provedor de dados: 5
Autores: Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for...
Tipo: Text
Palavras-chave: Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2919.pdf

Recherche d'effets biologiques de l'atrazine sur hémocytes d'huître creuse, Crassostrea gigas, in vivo et in vitro
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Thomas-guyon, Hélène; Lapegue, Sylvie; Bouilly, Karine; Renault, Tristan.
In the last decades, shellfish farming and in particular ostreiculture have developed in a significant way in France. Marennes-Oleron (Charente-Maritime) basin is the first French producer of oysters. But this area is also subjected to many recurring pollution, brought mainly by the Charente river.  The recrudescence using of herbicides in agriculture including atrazine implies the transfer towards the aquatic environment of new pollutants in estuarine areas. Moreover industrial rejections bring many heavy metals in these same estuarine areas.  It is appropriate of wondering on harmful effects of all these pollutants on the animals living in these areas, particularly oysters.  Bivalve molluscs have been postulated as ideal indicator organisms because of...
Tipo: Text
Palavras-chave: Atrazine; Flow cytometry; Haemocytes; Crassostrea gigas; Atrazine; Cytométrie de flux; Hémocytes; Crassostrea gigas.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/acte-834.pdf

Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan.
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR)....
Tipo: Text
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf

Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Bado Nilles, A; Gagnaire, Beatrice; Thomas-guyon, Helene; Le Floch, S; Renault, Tristan.
The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24 h contamination period and 10 of them showed significant modulations of...
Tipo: Text
Palavras-chave: Toxicity; PAHs; Pacific oyster; Spectrophotometry; Flow cytometry; Haemocyte activity.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4696.pdf

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile
Provedor de dados: 5
Autores: Seoane, Marta; González-fernández, Carmen; Soudant, Philippe; Huvet, Arnaud; Esperanza, Marta; Cid, Ángeles; Paul-pont, Ika.
Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their...
Tipo: Text
Palavras-chave: Microalgae; Microplastic; Flow cytometry; Cytotoxicity; Scanning electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00492/60415/63850.pdf

Cultured heart cells from oyster: an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin
Provedor de dados: 5
Autores: Droguet, Mickael; Devauchelle, Nicole; Pennec, Jean-pierre; Quinn, Brian; Dorange, Germaine.
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10(-6) M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4,...
Tipo: Text
Palavras-chave: Oyster; Heart cells; Flow cytometry; Tributyltin; Toxicity; Antifouling.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00089/20032/17688.pdf

In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families.
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf

Induction of apoptosis by UV in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that...
Tipo: Text
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf

Analysis of larval oyster grazing by flow cytometry
Provedor de dados: 5
Autores: Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene.
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice...
Tipo: Text
Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf

Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
Provedor de dados: 5
Autores: Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle.
Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to...
Tipo: Text
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf

Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
Provedor de dados: 5
Autores: Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity...
Tipo: Text
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf

A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes
Provedor de dados: 5
Autores: Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium.   Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf

Impact of the toxic dinoflagellate Alexandrium catenella on Pacific oyster reproductive output: application of flow cytometry assays on spermatozoa
Provedor de dados: 5
Autores: Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline; Miner, Philippe; Suquet, Marc; Lambert, Christophe; Soudant, Philippe.
The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only...
Tipo: Text
Palavras-chave: Harmful algal bloom; Sperm quality; Cellular parameters; Flow cytometry; Pacific oyster; Crassostrea gigas; Alexandrium catenella.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26853/24982.pdf

Étude chez l'huître creuse, Crassostrea gigas, des anomalies génomiques provoquées par l'exposition à des concentrations environnementales de diuron : caractérisation des atteintes, étude de leur héritabilité et conséquences pour la survie et la croissance des naissains
Provedor de dados: 5
Autores: Barranger, Audrey.
The aims of this PhD were to determine (1) the effects of the herbicide diuron on the genome of the oyster, Crassostrea gigas, (2) the possible transmission to the offspring of damaged DNA and (3) its consequences on oyster physiology. The genotoxicity of diuron and the vertical transmission of DNA damage were highlighted after parental exposure to environmental concentrations of diuron during the gametogenesis. Primary DNA lesions were detected in both somatic cells and germ cells of exposed genitors. Detection of the oxidized base 8-oxodGuo in gonads shows that exposure to diuron led to an oxidative stress which likely explains the observed DNA strand breaks in spermatozoa. The transmission of these DNA damage was confirmed by fluorescent in situ...
Tipo: Text
Palavras-chave: Crassostrea gigas; Herbicide; Diuron; Génotoxicité; Effets trans-générationnels; Cytogénétique; Test des comètes; Cytométrie en flux; Crassostrea gigas; Herbicide; Diuron; Genotoxicity; Trans-generational effects; Cytogenetic; Comet assay; Flow cytometry.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00271/38237/36419.pdf

Etude des effets de polluants sur les paramètres hémocytaires de l'huître creuse, Crassostrea gigas - Interactions entre environnement, mécanismes de défense et maladies infectieuses
Provedor de dados: 5
Autores: Gagnaire, Beatrice.
Shellfish industry is mostly realized in estuary zones, which are subjected to pollutions due to anthropic activities. The harmful effects of pollutants on animals inhabiting these estuarine zones are poorly known. Among these animals, Pacific oyster, Crassostrea gigas, may represent a model because they are sedentary and they filter water intensively. Among all physiological functions possibly disturbed by pollutants, defence mechanisms are poorly studied in bivalves. Moreover, animals presenting impaired defence mechanisms may be more sensitive to infectious diseases. In this context, effects of pollutants on hemocyte functions and on sensitiveness to diseases were tested in C. gigas. After adjusting the protocols for the monitoring of hemocyte...
Tipo: Text
Palavras-chave: Real time PCR; Flow cytometry; Pesticides; Pollutants; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster; Pathology; Immunotoxicology; PCR en temps réel; Cytométrie de flux; Pesticides; Polluants; Phagocytose; Hémocytes; Crassostrea gigas; Huître creuse; Pathologie; Immunotoxicologie.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-730.pdf

An Improved Protocol for Flow Cytometry Analysis of Phytoplankton Cultures and Natural Samples
Provedor de dados: 5
Autores: Marie, Dominique; Rigaut-jalabert, Fabienne; Vaulot, Daniel.
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of side scatter (a proxy of cell size) and fluorescence of natural pigments. We found that different marine phytoplankton species react differently to the action of Pluronic F68. In particular, photosynthetic prokaryotes are less sensitive than eukaryotes. Observed cell losses may result from cell lysis or from cell adhesion to the walls of plastic tubes that are commonly used for flow cytometry analysis....
Tipo: Text
Palavras-chave: Phytoplankton; Flow cytometry; Preservation; Pluronic F68; Glutaraldehyde.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00374/48493/48858.pdf

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone
Provedor de dados: 5
Autores: Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf

Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure
Provedor de dados: 5
Autores: Le Goic, Nelly; Hegaret, Helene; Boulais, Myrina; Beguel, Jean-philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe.
The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but...
Tipo: Text
Palavras-chave: Pacific oyster; Gamete quality; ROS production; Viability; Flow cytometry; Ecotoxicology.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00216/32760/31321.pdf

Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater
Provedor de dados: 5
Autores: Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979&amp;#305; in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf

Etude in vivo et in vitro des effets du mercure sur la réponse hémocytaire de l'huître creuse, Crassostrea gigas : développement de techniques évaluant les impacts de la pollution estuarienne
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
In the last decades, shellfish industry represents an important economic activity in France and particularly in Charente-Maritime. Marennes-Oleron basin is the first French area of oyster production, especially Pacific  oyster, Crassostrea gigas. But this area is also subjected to many pollution due to anthropic activities. Industrial wastes are sources of mercury pollution in the Charente river. The harmful effects of such contaminants on animals inhabiting these estuarine zones are poorly known, although many studies begin to be developed. Most of these studies use bivalve molluscs as biological indicators : these animals can bioaccumulate pollutants within their tissues by filtration. Moreover, they are sedentary so they cannot escape in the case of a...
Tipo: Text
Palavras-chave: Flow cytometry; Haemocytes; Mercury; Crassostrea gigas; Cytométrie de flux; Hémocytes; Mercure; Crassostrea gigas.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/acte-830.pdf

Amélioration des productions phytoplanctoniques en écloserie de mollusques : caractérisation des microalgues fourrage
Provedor de dados: 5
Autores: Robert, Rene; Chretiennot-dinet, Marie-josèphe; Kaas, Raymond; Martin-jezequel, Véronique; Moal, Jeanne; Le Coz, Jean-rene; Nicolas, Jean-louis; Bernard, Eudes; Connan, Jean-paul; Le Dean, Loic; Le Gourrierec, Gaetane; Leroy, Bertrand; Quere, Claudie.
in mollusc hatcheries. Consequently, we assessed themost commonly used species to establish their growth and size profiles, their cytomorphological, biochemical, biomolecular identification as well as their cytometer signs, and also their ability to grow under extreme conditions of temperature and salinity. This work aimed at matching the conformity of the strains used in Argenton (dispatched to most of the French commercial hatcheries) to those of a referenced culture collection. A previous screening preceded this study, because of the 45 species and/or clones held originally, Argenton kept no more than 15 species. As most of the latter are also represented in referenced collection, our strains are therefore correctly identified. On the other hand, the...
Tipo: Text
Palavras-chave: Ecological limits; Biomolecular profile; Flow cytometry; Biochemical composition; Cytomorphology; Phytoplankton; Mollusc; Hatchery; Ecotolérance; Profil biomoléculaire; Cytométrie en flux; Composition biochimique; Cytomorphologie; Phytoplancton; Mollusques; Ecloserie.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/rapport-1546.pdf

Multigenerational exposure of the microalga Tetraselmis suecica to diuron leads to spontaneous long-term strain adaptation.
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Jerome, Marc; Rouxel, Julien; Khelifi, Celia; Rince, Maeva; Burgeot, Thierry.
To investigate the ability of microalgae to develop stable, long-term resistance to herbicides, the marine microalga Tetraselmis suecica was exposed to the herbicide diuron (5 µg/L) for a 43-generation exposure period followed by a 12-generation depuration phase. During the first 25 generations, diuron-exposed cultures showed doubling times ranging from 1.95 to 2.6 days, which was 2 to 2.5-fold longer than control cultures. Between generations 25 and 38, during diuron exposure, two out of the three exposed cultures exhibited a spontaneous drop in doubling time. These results provided evidence of culture adaptation to diuron. To assess persistence of the diuron adaptation observed on growth performance, one of the adapted cultures (D3) was maintained for 12...
Tipo: Text
Palavras-chave: Microalgae; Diuron; Multigenerational; Adaptation; Flow cytometry.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00146/25718/23796.pdf

Trophic relationships between the farmed pearl oyster Pinctada margaritifera and its epibionts revealed by stable isotopes and feeding experiments
Provedor de dados: 5
Autores: Lacoste, Elise; Raimbault, Patrick; Harmelin-vivien, Mireille; Gaertner-mazouni, Nabila.
Bivalve cultures support a host of epibionts, mainly suspension feeders, which can compete for food resources with the cultivated bivalves. However, the magnitude of interspecific competition for food in bivalve aquaculture settings remains inconclusive, especially in tropical areas. We investigated the interactions for food between the farmed pearl oyster Pinctada margaritifera and its epibionts, using stable isotope analysis and feeding experiments. Inter-and intraspecific variations of delta C-13 and delta N-15 stable isotope ratios (SIRs) were determined for oysters in the presence or absence of epibionts. The diet of the most abundant epibionts, Herdmania momus and Didemnum sp., was specified using isotope measurements and flow cytometry during...
Tipo: Text
Palavras-chave: Ascidian; Biofouling; Trophic interactions; Stable isotope; Flow cytometry.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00664/77582/79564.pdf

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis
Provedor de dados: 5
Autores: Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major...
Tipo: Text
Palavras-chave: Baltic Sea; Macoma balthica; Neoplasia; Cytogenetics; Histology; Flow cytometry.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-2918.pdf

Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan.
Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster...
Tipo: Text
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf

Genomic abnormalities affecting mussels (Mytilus edulis-galloprovincialis) in France are related to ongoing neoplastic processes, evidenced by dual flow cytometry and cell monolayer analyses
Provedor de dados: 5
Autores: Benabdelmouna, Abdellah; Saunier, Alice; Ledu, Christophe; Travers, Marie-agnes; Morga, Benjamin.
In the context of the abnormal mass mortality of mussels in France since 2014, Flow CytoMetry (FCM) was used in 2015 and 2016 to study the DNA content and cell cycle characteristics of hemic circulating cells collected from 2000 mussels. The mussels were sampled from 12 wild and cultivated blue mussels stocks distributed along the French Atlantic coast from the south Brittany to Pertuis Charentais areas. During these surveys, various genetic abnormalities were frequently detected, and ploidy characteristics revealed contrasting profiles that corresponded to respective contrasting sanitary status, i.e. healthy mussels with high cytogenetic quality (HCQ) versus diseased mussels with low cytogenetic quality (LCQ). In the present work, FCM and hemocytology...
Tipo: Text
Palavras-chave: Mussels; Hemocytes; Flow cytometry; Hemocytology; Cytogenetic quality; Neoplasia.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00455/56623/58443.pdf

Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Frouin, Heloise; Moreau, Kevin; Thomas Guyon, Helene; Renault, Tristan.
The Pacific oyster, Crassostrea gigas, is extensively cultivated and represents an important economic activity. Oysters are reared in estuarine areas, subjected to various biotic and abiotic factors. One of the limiting factors in aquaculture is mortality outbreaks, which may limit oyster production, and the causes of these outbreaks are not completely understood. In this context, the effects of temperature and salinity on Pacific oyster, C. gigas, haemocytes, were studied. Haemocytes are the invertebrate blood cells and thus have been shown to be involved in defence mechanisms. Flow cytometry was used for monitoring several haemocyte parameters. An increase of temperature induced an increase of haemocyte mortality, in both in vitro and in vivo...
Tipo: Text
Palavras-chave: Cellular activity; Flow cytometry; Salinity; Temperature; Haemocyte; Crassostrea gigas; Pacific oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-934.pdf

Possible bloom of free trichomes in the Bay of Marseille, NW Mediterranean Sea: an anomaly evidenced by flow cytometry
Provedor de dados: 5
Autores: Zhao, Li; Denis, Michel; Barani, Aude; Beker, Beatriz; Mante, Claude; Xiao, Tian; Gregori, Gerald.
The composition of ultraphytoplankton (10 m) sampled in surface waters (1 m depth) was investigated during summer 2010 in the Bay of Marseille (NW Mediterranean) using flow cytometry. In addition to groups of Synechococcus, Prochlorococcus, picoeukaryotes and nanoeukaryotes, an unidentified additional cluster was observed. The particles forming this cluster had a high abundance (7 10(4) events mL(1)), with both orange and red fluorescence intensities like Synechococcus, but with a size signal larger than that of 2 m fluorospheres (beads). These unknown particles were sorted out by flow cytometry and then observed by epifluorescence microscopy: they appeared to be chain-forming microorganisms, just like trichomes of some diazotroph cyanobacteria with one...
Tipo: Text
Palavras-chave: Ultraphytoplankton; Trichomes; Bay of Marseille; Flow cytometry; Cell sorting.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00372/48292/48454.pdf

Cellular and molecular responses of haemocytes from Ostrea edulis during in vitro infection by the parasite Bonamia ostreae
Provedor de dados: 5
Autores: Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle.
Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used...
Tipo: Text
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf

Caractérisation morphologique et fonctionnelle des hémocytes chez l'huître plate, Ostrea edulis
Provedor de dados: 5
Autores: Xue, Quiggang.
The mechanism that bivalve molluscs react to infectious agents are important research subjects in the fIeld of disease control studies for these animals. While the selection of disease resistant populations have made decisive progression in some species, the mechanism behinds these resistant phenomena is not yet clear so far. However, hemocytes must represent one of the key factors in the reaction process. Based on this hypothesis, the structure and function of European flat oyster, Ostrea edulis, hemocytes have been investigated in aid of different techniques in the present study. ln addition, Pacific oyster, Crassostrea gigas, hemocytes have been analysed in certain aspects so as to provide some results for comparison. European flat oyster and Pacific...
Tipo: Text
Palavras-chave: Bivalvia; Ostrea edulis; Crassostrea gigas; Mécanismes de défense; Hémocytes; Granulocytes; Hyalinocytes; Séparation; Anticorps monoclonaux; Enzymes; Cytométrie de flux; Bivalvia; Ostrea edulis; Crassostrea gigas; Defence mechanisms; Hemocytes; Granulocytes; Hyalinocytes; Separation; Monoclonal antibodies; Enzymes; Flow cytometry.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00107/21820/19406.pdf

Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis
Provedor de dados: 5
Autores: Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text
Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf

Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf

Antioxidant Effects of Protocatechuic Acid, Ferulic Acid, and Caffeic Acid in Human Neutrophils Using a Fluorescent Substance
Provedor de dados: 77
Autores: Hyogo,Akiyo; Kobayashi,Toshihiro; Garcia del Saz,Eva; Seguchi,Harumichi.
Human neutrophils stimulated by phorbol myristate acetate (PMA), an activator of protein kinase C, produce active oxygen by NADPH oxidase in intracellular structures. We added succinimidyl ester of dichlorodihydrofluorescein diacetate (H2DCFDA), which first emits fluorescence when oxidized with active oxygen species, to neutrophils to produce active oxygen, in order to investigate the antioxidant effects of protocatechuic acid, ferulic acid, and caffeic acid which belong to polyphenols and are widely distributed among plants. Particularly, we focused on examining whether these substances capture and eliminate active oxygen inside or outside the neutrophil cytoplasm and whether these substances inhibit NADPH oxidase. Fluorescence microscopy demonstrated...
Tipo: Journal article
Palavras-chave: Antioxidant effects; Polyphenols; Human neutrophils; Flow cytometry.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300040

Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study
Provedor de dados: 77
Autores: Rosenberg,David R; Kernitsky,Jeremy R; Andrade,Catherine X; Ramirez,Valeria; Violant,Deborah; Nart,José.
Reports indicate that statins (cholesterol-lowering drugs), in addition to lowering cholesterol, have an immunomodulatory effect. This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effect of an atorvastatin-medicated dentifrice on CD4+ T cell proliferation. CD4+ T cell proliferation assays and peripheral blood mononuclear cell (PBMC) viability assays were conducted on PBMCs from healthy donors cultured under the following conditions: control, atorvastatin solution, atorvastatin-medicated dentifrice, and dentifrice without atorvastatin at concentrations of 1, 5, 10, 50 and 100 µM. A Generalized Equation Estimation (GEE) model was used to...
Tipo: Journal article
Palavras-chave: Atorvastatin calcium; Dentifrices; T cells; Flow cytometry.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200002

Immunophenotypic characterization of patients with American cutaneous leishmaniasis prior to and after treatment in Pernambuco, Brazil
Provedor de dados: 80
Autores: Almeida,AF; Castro,MCAB; Oliveira,AP; Souza,MA; Pereira,VRA.
Leishmania infections induce a specific activation of host immunological response, particularly characterized by T cell expansion. Studies indicate the importance of the balance between CD4+ and CD8+ T cells, in which the first ones would have their number reduced during the healing process. Meanwhile, CD25+ T cells have been associated with the suppression of the immune response. Since the immune response has an essential role in both healing and progression of diseases, this study aimed to identify the percentage of CD3+, CD4+, CD8+, CD16+ and CD25+ T cells in the peripheral blood of patients afflicted with American cutaneous leishmaniasis (ACL) - before and after treatment - and healthy controls. Peripheral blood was collected and transferred to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: American tegumentary leishmaniasis; Cellular immune response; Flow cytometry; Leishmania (Viannia) braziliensis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200016

Six new Phytophthora species from ITS Clade 7a including two sexually functional heterothallic hybrid species detected in natural ecosystems in Taiwan.
Provedor de dados: 16
Autores: Jung, T.; Jung, M.H.; Scanu, B.; Seress, D.; Kovács, G.M.; Maia, C.; Pérez-Sierra, A.; Chang, T.-T.; Chandelier, A.; Heungens, K.; Poucke, K. van; Abad-Campos, P.; Léon, M.; Cacciola, S.O.; Bakonyi, J.
During a survey of Phytophthora diversity in natural ecosystems in Taiwan six new species were detected. Multigene phylogeny based on the nuclear ITS, ß-tubulin and HSP90 and the mitochondrial cox1 and NADH1 gene sequences demonstrated that they belong to ITS Clade 7a with P. europaea, P. uniformis, P. rubi and P. cambivora being their closest relatives. All six new species differed from each other and from related species by a unique combination of morphological characters, the breeding system, cardinal temperatures and growth rates. Four homothallic species, P. attenuata, P. flexuosa, P. formosa and P. intricata, were isolated from rhizosphere soil of healthy forests of Fagus hayatae, Quercus glandulifera, Q. tarokoensis, Castanopsis carlesii,...
Tipo: Article / Letter to the editor
Palavras-chave: Biosecurity; Breeding systems; Evolution; Flow cytometry; Phylogeny; Phytophthora cambivora; Radiation.
Ano: 2017
URL: http://www.repository.naturalis.nl/record/618617

Flow Cytometric Analysis of Eimeria tenella Sporozoites Exposed to Nigericin, Monensin and Lasalocid In Vitro
Provedor de dados: 19
Autores: Hofmann, J.; Raether, W.; Ehrlich, K.
Palavras-chave: Flow cytometry; Polyether antibiotics; Eimeria tenella; Esterase activity; Membrane activity.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/185

Dexamethasone Regulates Macrophage and Cd4+Cd25+ Cell Numbers in the Chicken Spleen
Provedor de dados: 89
Autores: Calefi,AS; Quinteiro-Filho,WM; Fukushima,AR; Cruz,DSG da; Siqueira,A de; Salvagni,FA; Namazu,LB; Gomes,COMS; Ferreira,AJP; Palermo Neto,J.
Abstract Dexamethasone (DEX) is a corticoid hormone that is experimentally used to mimic the effects of increased levels of endogenous corticosterone observed during the stress response. Currently, stress is considered one of the major predisposing factors for diseases in the poultry industry. The aim of this study was to analyze the effects of DEX and/or of a 20-fold coccidial vaccine dose on leukocyte phenotypes in the spleen and cecal tonsils of chickens. Twenty specific-pathogen-free (SPF) Leghorn chickens were divided into four groups: a non-treated group (NT), a DEX-treated group (Dex), a vaccinated group (V) and a DEX-treated+vaccinated group (Dex+V). On experimental day (ED) 42, each bird in the vaccinated groups received a anti-coccidial vaccine....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coccidiosis; Eimeria; Avian immunology; Flow cytometry; Neuroimmunomodulation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100093

DNA content estimation of Fig and Black Mulberry using flow cytometry
Provedor de dados: 94
Autores: Dalkiliç,Zeynel; Dalkiliç,Gonca Günver.
Abstract  In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ficus carica; Morus nigra; DAPI; DNA content; Flow cytometry; Genome size.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200801

Nuclear DNA content and chromosome number in Brachiaria spp. genotypes
Provedor de dados: 124
Autores: Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Souza Sobrinho,Fausto; Davide,Lisete Chamma.
Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm bank; Cytogenetics; Flow cytometry; Forage.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000100008

Genome size and ploidy variations in pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) genotypes
Provedor de dados: 109
Autores: Animasaun,David Adedayo; Morakinyo,Joseph Akintade; Mustapha,Oba Toyin; Krishnamurthy,Rama.
Abstract Pennisetum glaucum (Pearl millet) and P. purpureum (Napier grass) are economically important members of the genus  Pennisetum . The knowledge of variability in chromosome number, size and genomic content of the species could provide clues on the mechanisms responsible for decrease or increase in genomic content in the evolutionary pathway. In the present study, twenty nine genotypes consisting of 24 pearl millet and 5 Napier grass were assessed for inter- and intra-variations in chromosome number, ploidy status and genome content. Conventional cytogenetic was used for chromosome counting and flow cytometry technique for assessing genomic contents of the genotypes. Pearl millet genotypes were diploid (2n = 14) while Napier grass were mainly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genome size; Flow cytometry; Napier grass; Pearl millet; Pennisetum; Ploidy analysis.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000400299

Active caspase-3 expression levels as bioindicator of individual radiosensitivity
Provedor de dados: 42
Autores: SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR.
ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649

Lymphocyte subset reference intervals in blood donors from northeastern Brazil
Provedor de dados: 42
Autores: TORRES,ALEX J.L.; CISNEIROS,PATRÍCIA; GUEDES,ROSA; GRASSI,MARIA FERNANDA R.; MEYER,ROBERTO; BENDICHO,MARIA T.; LOPES,TAIS G.S.L.; FÉLIX,GABRIELA; NETTO,EDUARDO M.; BRITES,CARLOS; ABE-SANDES,KIYOKO; BRANDÃO,CLAUDIO; ALCANTARA-NEVES,NEUZA; FREIRE,SONGELI M.
The reference intervals for leukocytes and lymphocytes currently used by most clinical laboratories present limitations as they are primarily derived from individuals of North American and European origin. The objective this study was to determine reference values for peripheral blood B lymphocytes, T lymphocyte subsets (CD4+, CD8+, naïve, memory, regulatory, TCRαβ and TCRγδ+) and NK cells from blood donors in Salvador-Bahia, Brazil. Results: The proportion of included male subjects was 73.7% and the median ages of males (34) and females (35) were found to be similar. Absolute counts total lymphocytes subsets to both gender was 1,956 (1,060-4,186) cells and relative values 34%. The T CD4+ and T CD8+ lymphocytes relative values was 51% (20-62) and 24%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocyte subsets; Reference values; Flow cytometry; Brazil; Blood donors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201019

rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)
Provedor de dados: 42
Autores: REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M.
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727

Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ)
Provedor de dados: 42
Autores: COELHO-SOUZA,SERGIO A.; ARAÚJO,FÁBIO V.; CURY,JULIANO C.; JESUS,HUGO E.; PEREIRA,GILBERTO C.; GUIMARÃES,JEAN R.D.; PEIXOTO,RAQUEL S.; DÁVILA,ALBERTO M.R.; ROSADO,ALEXANDRE S.
ABSTRACTUpwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability of Bacteria and Archaea in two stations of the Arraial do Cabo coastal region, one under upwelling pressure and another under anthropogenic pressure. In addition, biotic and abiotic variables were measured in surface and deep waters from three other stations between these stations. Six samplings were done during a year and adequately represented the degrees of upwelling and anthropogenic pressures to the system. Principal Component Analysis (PCA) showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial diversity; Leucine incorporation; Flow cytometry; Temperature; Nutrients availability; Brazilian Upwelling System.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401737

Karyotype characterization and nuclear DNA content measurement in Bromeliaceae: State of the art and future perspectives
Provedor de dados: 42
Autores: NUNES,ANDREI C.P.; CLARINDO,WELLINGTON R.
In Bromeliaceae, cytogenetic and flow cytometry analyses have been performed to clarify systematic and evolutionary aspects. Karyotyping approaches have shown the relatively high chromosome number, similar morphology and small size of the chromosomes. These facts have prevented a correct chromosome counting and characterization. Authors have established a basic chromosome number of x = 25 for Bromeliaceae. Recently, one karyomorphological analysis revealed that x = 25 is no longer the basic chromosome number, whose genome may have a polyploid origin. Besides cytogenetic characterization, the 2C DNA content of bromeliads has been measured. Nuclear DNA content has varied from 2C = 0.60 to 2C = 3.34 picograms. Thus, in relation to most angiosperms, the 2C DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allopolyploid; Bromeliads; Cytogenetics; Flow cytometry; Genome evolution.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401849

Hematological and immunological development from birth to six months of age in Holstein calves
Provedor de dados: 45
Autores: Baccili,C.C.; Oliveira,S.M.F.N. de; Costa,J.F.R; Massoco,C.O.; Pozzi,C.R.; Gomes,V.
ABSTRACT The hematological and immunological development of calves from birth to 6 months of age was performed by hemogram and cellular phenotype. Ten male Holstein calves were assessed in 13 moments: before colostrum intake (D0), every 2 days until the 10th day of life (D2 to D10), at the 15th day after birth (D15), and then monthly up to 6 months (D180). Calves presented hemoconcentration on day (D) 0 and showed a gradual decrease in hematimetric rates until D180. The inversion of the neutrophilic for lymphocytic profile was observed on D4. The percentage of CD3+ cells on D10 was higher than D30 up to D180. The number of CD4+ and CD8+ cells did not change between time points. The number of CD21+ lymphocytes was significantly higher at early time points...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Immune system; Leukogram; Immunophenotyping; Flow cytometry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601823

Regulatory T cells in dogs with multicentric lymphoma: peripheral blood quantification at diagnosis and after initial stage chemotherapy
Provedor de dados: 45
Autores: Munhoz,T.D.; Anai,L.A.; Fonseca,D.M.; Semolin,L.M.; Sueiro,F.R.; Tinucci-Costa,M.
Lymphoma is the most common hematopoietic malignancy in dogs and one of the most frequent among all neoplastic diseases in this species. It can occur in several anatomical locations with distinct histological and immunophenotypes. Depending on the host immune response towards the tumor, prognosis information could be collected. Because its well established immunosuppressant, antitumor activity, the function of regulatory T cells (Tregs) in canine neoplasias has been investigated. In this study, we sought to quantify, using flow cytometry, the Tregs subpopulation in peripheral blood of healthy dogs (10) and in those diagnosed with type-B (14) and type-T (8) multicentric lymphoma before (at diagnosis) and after the first cycle (5-week) of 19-week...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Multicentric lymphoma; Flow cytometry; Regulatory T cells.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000100001

Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?
Provedor de dados: 48
Autores: Ontsouka,Edgar Corneille; Bertschi,Janique Sabina; Huang,Xiao; Lüthi,Michael; Müller,Stefan; Albrecht,Christiane.
BACKGROUND: Mammary cell cultures are convenient tools for in vitro studies of mammary gland biology. However, the heterogeneity of mammary cell types, e.g., glandular milk secretory epithelial or myoepithelial cells, often complicates the interpretation of cell-based data. The present study was undertaken to determine the relevance of bovine primary mammary epithelial cells isolated from American Holstein (bMEC US) or Swiss Holstein-Friesian (bMEC CH) cows, and of primary bovine mammary alveolar epithelial cells stably transfected with simian virus-40 (SV-40) large T-antigen (MAC-T) for in vitro analyses. This was evaluated by testing their expression pattern of cytokeratin (CK) 7, 18, 19, vimentin, and &amp;#945;-smooth muscle actin (&amp;#945;-SMA....
Tipo: Journal article
Palavras-chave: Cell characterization; Flow cytometry; Gene expression; Lactation marker; Mammalian; Mammary epithelial cell.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100001

Chromosome numbers, meiotic abnormalities, and 2n pollen formation in accessions of the wild species Chrysolaena flexuosa (Vernonieae, Compositae) from its distribution range in Argentina
Provedor de dados: 51
Autores: Echeverría,María Lis; Camadro,Elsa Lucila.
Chrysolaena flexuosa is a South American species of potential ornamental value (basic chromosome number of x=10). Diploid (n=10) and tetraploid (n=20) cytotypes have been reported for its distribution area, although one hexaploid (n=30-32ca.) cytotype has been reported for its most southern distribution. To investigate if ploidy and latitude are positively related in Ch. flexuosa natural populations and if sexual polyploidization could have had a role in the origin of the polyploid cytotypes, we determined chromosome numbers, DNA content, and pollen viability and size, and analyzed microsporogenesis in samples of seven Argentinian accessions. Two of the northeastern accessions were diploid and one was tetraploid, whereas the four southeastern accessions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyploid cytotypes; DNA content; Flow cytometry; Meiotic abnormalities; Abnormal spindle orientation; 2n pollen.
Ano: 2017
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722017000400010

Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
Provedor de dados: 52
Autores: Santos,Neyliane Gonçalves dos; Amaral,Ademir; Cavalcanti,Mariana Brayner; Neves,Maria Amélia Batista; Machado,Cíntia Gonsalves de Faria.
The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Combined therapy; Radiation effect; P-glycoprotein; Multidrug resistance; Functional analysis; Flow cytometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection
Provedor de dados: 55
Autores: Palma,Priscila F.R.; Baggio,Giovana L.; Spada,Celso; Silva,Renata da; Ferreira,Silvia Inês A.C.P.; Treitinger,Aricio.
Evaluation of apoptosis by flow cytometry is generally accomplished by methods that use annexin V-FITC as vital dye, which access phosphatidylserine exposed on the external membrane at the beginning of this process. In addition, the concomitant use of propidium iodide makes possible to verify the characteristic nuclear alterations in the late stages of apoptosis, as a consequence of the increase in membrane permeability. On the other hand, the use of calcein-AM in association with ethidium homodimer (EthD-1) allows the evaluation of cell apoptosis through detection of esterase activity and cellular membrane physical and chemical alterations. The aim of this study was to compare the sensibility of calcein-AM and EthD-1 with annexin V-FITC and propidium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Flow cytometry; Annexin V; Propidium iodide; Calcein-AM; Ethidium homodimer.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200003

Evaluation of urinalysis parameters to predict urinary-tract infection
Provedor de dados: 55
Autores: Santos,Juliana Conrad dos; Weber,Liliana Portal; Perez,Leandro Reus Rodrigues.
We evaluated the performance of automated-flow cytometry, urinalysis dipsticks and microscopic urine sediment analysis as predictors of urinary tract infection. Urine cultures were used as a reference method for comparison. Six-hundred-seventy-five urine samples from hospitalized and not hospitalized patients attended at Hospital Mãe de Deus, Porto Alegre, in south Brazil, were included in the study. Among the individual measures analyzed, intense bacteriuria in the microscopic analysis of urinary sediment gave an accuracy of 92.9%. A combination between intense bacteriuria (microscopic analysis) and &gt;20 leukocytes per µL of urine (flow cytometry) gave a higher accuracy (97.3%). We conclude that though it is laborious, microscopic urinalysis is a good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urinary tract infection; Urinalysis; Flow cytometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500008

High degree of concordance between ﬂow cytometry and geno2pheno methods for HIV-1 tropism determination in proviral DNA
Provedor de dados: 55
Autores: Torres,Alex José Leite; Brígido,Luis Fernando de Macedo; Abrahão,Marcos Herculano Nunes; Angelo,Ana Luiza Dias; Ferreira,Gilcivaldo de Jesus; Coelho,Luana Portes; Ferreira,João Leandro; Jorge,Célia Regina Mayoral Pedroso; Martins Netto,Eduardo; Brites,Carlos.
Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a ﬂow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identiﬁed by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identiﬁcation of X4 and R5 tropic viruses was attempted by geno2pheno...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Tropism; Flow cytometry; Geno2pheno.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200163

Compositional changes of PBL population in patients with chronic hepatitis B virus infection
Provedor de dados: 55
Autores: Alireza,Khabiri; Mohsen,Abolhassani; Sudabeh,Aslani; Hiedeh,Darabi; Farideh,Bagheri; Mohammad-Hossein,Alimohammadian.
In this report we have analysed the peripheral blood lymphocyte of several patients with chronic hepatitis B virus infection with flow cytometry. Based on the presence and absence of the HBeAb, patients were divided into two groups. In both, all the patients were HBsAg positive with normal range of serum alanine aminotranferase (23.9 ± 17.8). We have found that the immunophenotypic profiles of patients were different from healthy donors with significant decrease in CD3+ T cells, specially CD8+ T cells and a siginificant increase in the CD19+ B cells. The differences were seen in other subset of T cells (CD4+) or NK cells (CD56+/CD16+ ) and HLA-DR markers were not significant. When the phenotypic profiles of both groups were compared with each other, such...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B virus; Chronic HBV infection; HBsAg; HBeAg; HBeAb; Lymphocyte subset; Flow cytometry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600009

Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies
Provedor de dados: 55
Autores: De Gaspari,Elizabeth N.; Zollinger,Wendell D.
Determining the profile of antigen expression among meningococci is important for epidemiologic surveillance and vaccine development. To this end, two new mouse monoclonal antibodies (MAbs) have been derived against Neisseria meningitidis proteins (class 5). The MAbs were reactive against outer membrane antigens and were bactericidal. Selected anti-class 5 MAbs [(5.1)-3E6-2; (5.3)-3BH4-C7; (5.4)-1BG11-C7; (5.5)-3DH-F5G9 also 5F1F4-T3(5.c)], and the two new monoclonal antibodies C14F10Br2 (5.8) and 7F11B5Br3 (5.9), were then tested against different meningococcal strains, (63 strains of serogroup A, 60 strains of serogroup C (from 1972 to 1974); and 136 strains of serogroup B (from 1992) meningococci). Our results demonstrated that the expression of class 5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria meningitidis; Monoclonal antibodies; Outer membrane protein; Flow cytometry.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300007

A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
Provedor de dados: 56
Autores: Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606

Immunophenotype of normal and leukemic bone marrow B-precursors in a Brazilian population. A comparative analysis by quantitative fluorescence cytometry
Provedor de dados: 56
Autores: Rego,E.M.; Garcia,A.B.; Carneiro,J.J.; Falcão,R.P.
The distinction between normal and leukemic bone marrow (BM) B-precursors is essential for the diagnosis and treatment monitoring of acute lymphoblastic leukemia (ALL). In order to evaluate the potential use of quantitative fluorescence cytometry (QFC) for this distinction, we studied 21 normal individuals and 40 patients with CD10+ ALL. We characterized the age-related changes of the CD10, CD19, TdT, CD34 and CD79a densities in normal and leukemic BM. Compared to normal adults, the B-precursors from normal children expressed significantly lower values of CD34-specific antibody binding capacity (SABC) (median value of 86.6 vs 160.2 arbitrary units (a.u.) in children and adults, respectively). No significant age-related difference was observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic leukemia; Lymphocytes; Quantitative fluorescence cytometry; Flow cytometry; Age-related changes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200005

Trypan blue exclusion assay by flow cytometry
Provedor de dados: 56
Autores: Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.
Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells
Provedor de dados: 56
Autores: Hu,T.; Shi,J.; Jiao,X.; Zhou,J.; Yin,X.
Phosphatidylserine (PS) exposure occurs during the cell death program and fluorescein-labeled lactadherin permits the detection of PS exposure earlier than annexin V in suspended cell lines. Adherent cell lines were studied for this apoptosis-associated phenomenon to determine if PS probing methods are reliable because specific membrane damage may occur during harvesting. Apoptosis was induced in the human tongue squamous carcinoma cell line (Tca8113) and the adenoid cystic carcinoma cell line (ACC-2) by arsenic trioxide. Cells were harvested with a modified procedure and labeled with lactadherin and/or annexin V. PS exposure was localized by confocal microscopy and apoptosis was quantified by flow cytometry. The detachment procedure without trypsinization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adherent cells; Annexin V; Apoptosis; Flow cytometry; Lactadherin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900002

Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis
Provedor de dados: 56
Autores: Godoy,C.R.T.; Levy,D.; Giampaoli,V.; Chamone,D.A.F.; Bydlowski,S.P.; Pereira,J.
We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P&lt;0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circulating endothelial cells; Vascular endothelial growth factor; Chronic myeloid leukemia; Flow cytometry; Angiogenesis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509

Adhesion molecule profiles of B-cell non-Hodgkin's lymphomas in the leukemic phase
Provedor de dados: 56
Autores: Matos,D.M.; Rizzatti,E.G.; Garcia,A.B.; Gallo,D.A.P.; Falcão,R.P.
We evaluated the expression of 10 adhesion molecules on peripheral blood tumor cells of 17 patients with chronic lymphocytic leukemia, 17 with mantle-cell lymphoma, and 13 with nodal or splenic marginal B-cell lymphoma, all in the leukemic phase and before the beginning of any therapy. The diagnosis of B-cell non-Hodgkin's lymphomas was based on cytological, histological, immunophenotypic, and molecular biology methods. The mean fluorescence intensity of the adhesion molecules in tumor cells was measured by flow cytometry of CD19-positive cells and differed amongst the types of lymphomas. Comparison of chronic lymphocytic leukemia and mantle-cell lymphoma showed that the former presented a higher expression of CD11c and CD49c, and a lower expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-Hodgkin's lymphoma; Adhesion molecules; Flow cytometry; CD49c/CD49d.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001000011

Asynchronous expression of myeloid antigens in leukemic cells in a PML/RARalpha transgenic mouse model
Provedor de dados: 56
Autores: Santana,B.A.A.; Pintão,M.C.; Lima,R.S. Abreu e; Scheucher,P.S.; Santos,G.A.S.; Garcia,A.B.; Falcão,R.P.; Rego,E.M.
Acute promyelocytic leukemia (APL) is characterized by the expansion of blasts that resemble morphologically promyelocytes and harbor a chromosomal translocation involving the retinoic acid receptor a (RARa) and the promyelocytic leukemia (PML) genes on chromosomes 17 and 15, respectively. The expression of the PML/RARa fusion gene is essential for APL genesis. In fact, transgenic mice (TM) expressing PML/RARa develop a form of leukemia that mimics the hematological findings of human APL. Leukemia is diagnosed after a long latency (approximately 12 months) during which no hematological abnormality is detected in peripheral blood (pre-leukemic phase). In humans, immunophenotypic analysis of APL blasts revealed distinct features; however, the precise...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Acute promyelocytic leukemia; Acute myelogenous leukemia; Transgenic mice; Flow cytometry; Leukemogenesis.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500008

In vitro modulation of Bcl-2 levels in small cell lung cancer cells: effects on cell viability
Provedor de dados: 56
Autores: Santos,A.O.; Pereira,J.P.; Pedroso de Lima,M.C.; Simões,S.; Moreira,J.N.
Small cell lung cancer (SCLC) is an aggressive disease, representing 15% of all cases of lung cancer, has high metastatic potential and low prognosis that urgently demands the development of novel therapeutic approaches. One of the proposed approaches has been the down-regulation of BCL2, with poorly clarified and controversial therapeutic value regarding SCLC. The use of anti-BCL2 small interfering RNA (siRNA) in SCLC has never been reported. The aim of the present study was to select and test the in vitro efficacy of anti-BCL2 siRNA sequences against the protein and mRNA levels of SCLC cells, and their effects on cytotoxicity and chemosensitization. Two anti-BCL2 siRNAs and the anti-BCL2 G3139 oligodeoxynucleotide (ODN) were evaluated in SCLC cells by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Small cell lung cancer; Gene silencing; Bcl-2; SiRNA; Antisense ODN; Flow cytometry; Chemosensitization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000013

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis
Provedor de dados: 56
Autores: Bertho,A.L.; Santiago,M.A.; Da-Cruz,A.M.; Coutinho,S.G.
Human localized cutaneous leishmaniasis (LCL), induced by Leishmania braziliensis, ranges from a clinically mild, self-healing disease with localized cutaneous lesions to severe forms which can present secondary metastatic lesions. The T cell-mediated immune response is extremely important to define the outcome of the disease; however, the underlying mechanisms involved are not fully understood. A flow cytometric analysis of incorporation of 7-amino actinomycin D and CD4+ or CD8+ T cell surface phenotyping was used to determine whether different frequencies of early apoptosis or accidental cell death occur at different stages of LCL lesions. When all cells obtained from a biopsy sample were analyzed, larger numbers of early apoptotic and dead cells were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Flow cytometry; Human leishmaniasis; Cell death; T cells.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300010

CD19+ B cell subsets in the peripheral blood and skin lesions of psoriasis patients and their correlations with disease severity
Provedor de dados: 56
Autores: Lu,J.; Ding,Y.; Yi,X.; Zheng,J.
T lymphocytes are important in the pathogenesis of psoriasis, and increasing evidence indicates that B cells also play an important role. The mechanisms of action, however, remain unclear. We evaluated the ratios of CD19+ B cells in peripheral blood mononuclear cells (PBMCs) from 157 patients with psoriasis (65 patients with psoriasis vulgaris, 32 patients with erythrodermic psoriasis, 30 patients with arthropathic psoriasis, and 30 patients with pustular psoriasis) and 35 healthy controls (HCs). Ratios of CD19+ B cells in skin lesions were compared with non-lesions in 7 erythrodermic psoriasis patients. The Psoriasis Area Severity Index (PASI) was used to measure disease severity. CD19+ B cell ratios in PBMCs from psoriasis vulgaris (at both the active...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psoriasis; B-lymphocytes; B-lymphocyte subsets; Psoriasis Area Severity Index (PASI); Flow cytometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900706

Rhein induces apoptosis of human gastric cancer SGC-7901 cells via an intrinsic mitochondrial pathway
Provedor de dados: 56
Autores: Li,Yiwen; Xu,Yuqing; Lei,Bo; Wang,Wenxiu; Ge,Xin; Li,Jingrui.
Rhein is a primary anthraquinone found in the roots of a traditional Chinese herb, rhubarb, and has been shown to have some anticancer effects. The aim of the present study was to investigate the effect of rhein on the apoptosis of the human gastric cancer line SGC-7901 and to identify the mechanism involved. SGC-7901 cells were cultured and treated with rhein (0, 50, 100, 150, and 200 µM) for 24, 48, or 72 h. Relative cell viability assessed by the MTT assay after treatment was 100, 99, 85, 79, 63% for 24 h; 100, 98, 80, 51, 37% for 48 h, and 100, 97, 60, 36, 15% for 72 h, respectively. Cell apoptosis was detected with TUNEL staining and quantified with flow cytometry using annexin FITC-PI staining at 48 h after 100, 200 and 300 µm rhein. The percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhein; SGC-7901 cells; MTT; TUNEL; Flow cytometry; Apoptosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100009

Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
Provedor de dados: 56
Autores: Machado,C.G.; Calado,R.T.; Garcia,A.B.; Falcão,R.P.
The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P-glycoprotein; Multidrug resistance; Lymphocytes; Rhodamine 123; Flow cytometry.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean
Provedor de dados: 58
Autores: Andrade,Luciana; Gonzalez,Alessandra M.; Rezende,Carlos Eduardo; Suzuki,Marina; Valentin,Jean Louis; Paranhos,Rodolfo.
Bacterioplankton was studied in a large area of Southwest Atlantic Ocean between 13 and 25ºS and 28 and 42ºW. Samples were collected in 108 stations at 20 m depth. Bacteria were enumerated by flow cytometry after nucleic acid staining with syto13 and two subgroups were differentiated: low nucleic acid content (LNA) and high nucleic acid content (HNA) bacteria. Total bacterial numbers varied from 0.37 to 5.53 10(5) cells mL-1. HNA cells represented 15 to 70% of the total number while LNA cells represented 30 to 85%. Heterotrophic bacterial production was determined by incorporation of tritiated leucine and ranged from 2.7 to 171.07 ng C L-1 h-1. No significant correlation was found between abundance and production. Nevertheless with support of multivariate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial abundance; HNA bacteria; LNA bacteria; Flow cytometry; Southwest Atlantic Ocean.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200028

Goat umbilical cord cells are permissive to small ruminant lentivirus infection in vitro
Provedor de dados: 58
Autores: Martins,Gabrielle R.; Marinho,Rebeca C.; Bezerra Junior,Rosivaldo Q.; Alves,Antoniel de O.; Câmara,Lilia M.C.; Albuquerque-Pinto,Luiz C.; Teixeira,Maria F. da S.
Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CAEV; MVV; Cell culture; MSC; Flow cytometry.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100125

Heterotrophic bacteria abundances in Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil)
Provedor de dados: 58
Autores: Gonzalez,Alessandra M.; Paranhos,Rodolfo; Lutterbach,Márcia S.
The Rodrigo de Freitas Lagoon (RJ, Brazil) is an important coastal ecosystem that has been submitted to an accelerated degradation process. The aim of this work was to determine the abundance and the spatial distribution of total heterotrophic (by flow cytometry) and cultivated bacteria ("pour plate" method on R2A agar). Another objective was to evaluate the lagoon's influence on water quality of Ipanema and Leblon beaches. Physical and chemical data were acquired too. Sub-superficial water samples were taken monthly, from December 1999 to October 2000. On lagoon, the cultivated bacteria abundance varied from 6.9x10(5) to 5.0x10(7) CFU.100 mL-1. On Ipanema and Leblon beaches, this parameter yielded 1.4x10(5) and 2.8x10(6) CFU.100 mL-1, respectively. Total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterioplankton; Flow cytometry; Eutrophication; Water pollution; Rodrigo de Freitas Lagoon.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400005

Immune response elicited by the oral administration of an intermediate strain of IBDV in chickens
Provedor de dados: 58
Autores: Carballeda,Juan Manuel; Zoth,Silvina Chimeno; Gómez,Evangelina; Lucero,María Soledad; Gravisaco,María José; Berinstein,Analía.
The immune response elicited by the oral inoculation of an intermediate strain of infectious bursal disease virus was studied in chickens. A strong over expression of IL-6, IL-8, IFNα and IFNγ was observed in bursa at 3 days post inoculation together with an increase in splenic NO2 release. An influx of T-lymphocytes was also detected.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious Bursal Disease virus; Innate immunity; Avian cytokines; Flow cytometry; RT-qPCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400049

In vitro anticancer activity of microbial isolates from diverse habitats
Provedor de dados: 60
Autores: Thomas,Angel Treasa; Rao,Josyula Venkata; Subrahmanyam,Volety Mallikarjuna; Chandrashekhar,Hariharapura Raghu; Maliyakkal,Naseer; Kisan,Tukaram Kedar; Joseph,Alex; Udupa,Nayanabhirama.
Extracts from natural products, especially microorganisms, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Identification of microbial strains having promising biological activities and purifying the bio-molecules responsible for the activities, have led to the discovery of many bioactive molecules. Extracellular, as well as intracellular, extracts of the metabolites of thirty-six bacterial and twenty-four fungal isolates, grown under unusual conditions such as high temperature, high salt and low sugar concentrations, were in vitro tested for their cytotoxic potential on various cancer cell lines. The extracts were screened on HeLa and MCF-7 cell lines to study the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anticancer; Cytotoxicity; Flow cytometry; Microbial extracts; MTT assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200009

Effects of different concentrations of sodium azide and chloramphenicol on the preservation of raw milk samples
Provedor de dados: 65
Autores: Vancin,Franciele Rampazzo; Webber,Bruna; Bondan,Carlos; Daroit,Luciane; Santos,Luciana Ruschel dos; Rodrigues,Laura Beatriz.
ABSTRACT: The preservation of milk samples for microbiological analyses by the Brazilian Network of Milk Quality Control Laboratories requires the addition of preservatives to maintain the microbiota from the time of sample collection to the moment of analysis. The number of microorganisms can change as a result of the active ingredients and concentration of the preservative, as well as due to interactions between the preservatives, incubation time, and packaging temperature. The objective of this research was to evaluate the conservation potential of different concentrations of sodium azide and chloramphenicol on the analytical shelf life of milk samples. Two farms were selected, one with a low bacterial count and one with a high bacterial count. The milk...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Azidiol; Individual bacterial count; Flow cytometry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200750