Consulta: subjectFacets:"Functional genomics"
Registros recuperados: 7
Data/hora: 01/06/2024 07:48:28

Global analysis of gene function in mammals: Integration of physical, mutational and expression strategies
Provedor de dados: 69
Autores: Schimenti,John.
The field of Genetics is undergoing tumultuous change after nearly a century of standard approaches to genetic analysis. The Human Genome Project is providing tools and technologies that are changing the ways that we pursue an understanding of gene function, which is the underlying goal in modern and traditional genetics. In this paper, I overview the directions of the genome project as they relate to gene function analysis in mice and humans, and how various modern technologies are coalescing to address this in a powerful way.
Tipo: Journal article
Palavras-chave: Gene expression; ESTs; Mutagenesis; Genome; Functional genomics.
Ano: 1998
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100005

T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics
Provedor de dados: 69
Autores: Radhamony,Resmi Nath; Mohan Prasad,Anand; Srinivasan,Ramamurthy.
With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using...
Tipo: Journal article
Palavras-chave: Arabidopsis; Functional genomics; Insertional mutagenesis; Promoter trapping; T-DNA tagging.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Provedor de dados: 74
Autores: Pereira,Wendell Jacinto; Melo,Arthur Tavares de Oliveira; Coelho,Alexandre Siqueira Guedes; Rodrigues,Fabiana Aparecida; Mamidi,Sujan; Alencar,Sérgio Amorim de; Lanna,Anna Cristina; Valdisser,Paula Arielle Mendes Ribeiro; Brondani,Claudio; Nascimento-Júnior,Ivanildo Ramalho do; Borba,Tereza Cristina de Oliveira; Vianello,Rosana Pereira.
Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Water deficit; Functional genomics; Single nucleotide polymorphism (SNP).
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309

Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text
Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf

Combined functional genomics and genetic approach in oyster to identify summer mortality-resistant markers
Provedor de dados: 5
Autores: Huvet, Arnaud; Lapegue, Sylvie.
The oyster Crassostrea gigas has global distribution and for the past several years the highest annual production of any freshwater or marine organism. Economic importance of oysters motivates a great deal of biological research, which provides the most immediate scientific rationales for sequencing ESTs and developing genomic tools.
Tipo: Text
Palavras-chave: Environmental genomics; Comparative genomics; Functional genomics; Markers; Summer mortalities; Genetic; Oysters; Crassostrea gigas.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4006.PDF

A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
Provedor de dados: 48
Autores: MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010

Cracking in sweet cherries: A comprehensive review from a physiological, molecular, and genomic perspective
Provedor de dados: 117
Autores: Balbontín,Cristián; Ayala,Héctor; M. Bastías,Richard; Tapia,Gerardo; Ellena,Miguel; Torres,Carolina; Yuri,José Antonio; Quero-García,José; Ríos,Juan Carlos; Silva,Herman.
Rain-induced cracking in fruits of sweet cherry (Prunus avium L.) is a problem in most producing areas of the world and causes significant economic losses. Different orchard management practices have been employed to reduce the severity of this problem, although a complete solution is not yet available. Fruit cracking is a complex phenomenon and there are many factors that seem to be involved in its development. During the last decade, genomic and biochemical approaches have provided new insights on the different mechanisms that could be involved in the differential susceptibility shown by commercial cultivars. For instance, sweet cherry genome and transcriptome sequencing information have provided new opportunities to study the expression and structure of...
Tipo: Journal article
Palavras-chave: Physiology; Functional genomics; RNA seq.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100010