Consulta: subjectFacets:"Gag"
Registros recuperados: 2
Data/hora: 07/06/2024 01:46:26

Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages
Provedor de dados: 5
Autores: Mariani, Charlotte; Desdouits, Marion; Favard, Cyril; Benaroch, Philippe; Muriaux, Delphine M.
HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is...
Tipo: Text
Palavras-chave: HIV-1; Lipids; Assembly; Gag; CD4(+) T cells; Macrophages.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00344/45490/45040.pdf

A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
Provedor de dados: 48
Autores: Acharya,Arpan; Vaniawala,Salil; Shah,Parth; Parekh,Harsh; Misra,Rabindra Nath; Wani,Minal; Mukhopadhyaya,Pratap N.
BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p&amp;#8201;&lt;&amp;#8201;0.001). Lower limit of detection of the test protocol was 50...
Tipo: Journal article
Palavras-chave: HIV-1; Gag; SYBR Green; RT PCR.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100022