Consulta: subjectFacets:"Gene"
Registros recuperados: 55
Data/hora: 01/06/2024 04:25:56

Between the cross and the sword: the crisis of the gene concept
Provedor de dados: 74
Autores: El-Hani,Charbel Niño.
Challenges to the gene concept have shown the difficulty of preserving the classical molecular concept, according to which a gene is a stretch of DNA encoding a functional product (polypeptide or RNA). The main difficulties are related to the overlaying of the Mendelian idea of the gene as a ‘unit’: the interpretation of genes as structural and/or functional units in the genome is challenged by evidence showing the complexity and diversity of genomic organization. This paper discusses the difficulties faced by the classical molecular concept and addresses alternatives to it. Among the alternatives, it considers distinctions between different gene concepts, such as that between the ‘molecular’ and the ‘evolutionary’ gene, or between ‘gene-P’ (the gene as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene; Classical molecular gene concept; Mendelian gene; Genomic complexity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300001

A SNP in 5′ untranslated region of CD40 gene is associated with an increased risk of ischemic stroke in a Chinese population: a case-control study
Provedor de dados: 74
Autores: Huang,Hua-Tuo; Guo,Jing; Xiang,Yang; Chen,Jian-Ming; Luo,Hong-Cheng; Meng,Lan-Qing; Wei,Ye-Sheng.
Abstract Cluster of differentiation 40 (CD40), the receptor for CD154, is a member of the tumor necrosis factor (TNF) receptor superfamily. Several studies have been conducted to investigate the effect of the CD40 rs1883832 polymorphism on atherosclerotic disease in different population; however, inconsistent results were obtained. In this study, we investigated the association of four polymorphisms (rs1883832, rs13040307, rs752118 and rs3765459) of CD40 gene and their effect on CD40 expression with the risk of ischemic stroke (IS) in a Chinese population. Three hundred and eighty patients with IS and 450 control subjects were included in the study. The CD40 polymorphisms were discriminated by Snapshot SNP genotyping assay. Serum soluble CD40 (sCD40)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CD40; Gene; Polymorphism; Ischemic stroke.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300442

Replication of TCF7L2 rs7903146 association with type 2 diabetes in an Iranian population
Provedor de dados: 74
Autores: Amoli,Mahsa M.; Amiri,Parvin; Tavakkoly-Bazzaz,Javad; Charmchi,Elham; Hafeziyeh,Jila; Keramatipour,Mohammad; Abiri,Maryam; Ranjbar,Shirin Hasani; Larijani,Bagher.
The transcription factor 7-like 2 gene (TCF7L2) rs7903146 T allele is constantly associated with Type 2 diabetes in various populations and ethnic groups. Nevertheless, this has not been observed in two studies involving Arab populations. The aim of the present study was to investigate the association between TCF7L2 rs7903146 in an Iranian population. Type 2 diabetes patients (N = 258) and normal healthy control subjects (N = 168) from the same area, were examined. The ARMS-PCR (Amplification Refractory Mutation System) technique, subsequently validated by direct sequencing, was used for genotyping. Allele and genotype frequencies were significantly different between patients and controls TT vs. CT + CC [p 0.0081 OR 3.4 95%CI (1.27-11.9)] and T vs. C...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TCF7L2; Gene; Polymorphism; T2DM.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300010

TALEN uma ferramenta na edição de genomas.
Provedor de dados: 14
Autores: VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.
Nucleases com efetores do tipo ativador transcricional (do inglês “transcription activator-like effector nucleases” – TALENs) é uma das técnicas incluídas nas chamadas Novas Tecnologias no Melhoramento de Plantas (do inglês “New Plant Breeding Techniques – NPBT”), as quais abrangem um conjunto de novas metodologias e abordagens que diferem da estratégia de melhoramento genético utilizando as técnicas de transgenia clássica que foram desenvolvidas na década de 1990. A tecnologia TALENs apoia-se em novos conhecimentos sobre os processos celulares de expressão e regulação gênica e tanto podem se apresentar como um refinamento das técnicas de modificação por transgenia clássica quanto podem, sob alguns aspectos, produzir eventos de transformação genética...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Melhoramento genético.; Genética; Gene; Genoma.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1037915

O Mecanismo de regulação da FBN e sua diversidade.
Provedor de dados: 14
Autores: TEIXEIRA, K. R. dos S.
Regulação da FBN no grupo dos rhizobios. Regulação da FBN em azospirillun brasiliense. Regulação da FBN em herbaspirillun seropedicae. Regulação da FBN em acetobacter diazotrophicus.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Fixação biológica de nitrogênio; FBN; Acetobacter diazotrophicus.; Genética.; Gene; Azospirillum brasilense; Herbaspirillum seropedicae.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624245

Utilização de ferramentas computacionais para análise de expressão de genes.
Provedor de dados: 14
Autores: NARCISO, M. G.; BEVITORI, R.
No âmbito da genômica funcional, o estudo do transcriptoma pode auxiliar na elucidação das funções dos genes. Isto é possível devido ao conhecimento existente sobre expressão gênica de que ela ocorre de maneira diferenciada nos diferentes tecidos, nas diferentes fases fenológicas, em geral, nas diferentes situações em que o organismo se encontra. Entretanto, o grande volume de dados gerados pelo NGS requer as ferramentas computacionais para serem analisados. Nesse sentido, um dos objetivos desta publicação é demonstrar a utilização de dois programas de bioinformática que são amplamente utilizados na análise de transcriptoma: Tophat e Cufflinks.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Sequenciamento; Gene.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1003470

Caracterização do gene 25k FP de Anticarsia gemmatalis nucleopolyhedrovirus.
Provedor de dados: 14
Autores: SOUZA, M. L. de; CASTRO, M. E. B. de; SIHLER, W.; DALMOLIN, C. C.; PEDRINI, M. R. da S.; SILVA, F. R. da.
bitstream/CENARGEN/24691/1/bp044.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Anticarsia gemmatalis nucleopolyhedrovirus; Produção in vitro; Sequenciamento.; Controle Biológico; Gene; Identificação; Lagarta.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/185380

Transformação de plantas.
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain new varieties. Genetic transformation is the targed organism using non sexual methods. Transgenic plants can be defined as those the present genes different from the original genoma obtained by techniques of genetic engineering. The transformation stages consist of the identification isolation, cloning and introduction of gene into the cells. There are several transformation methods, and the choice...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Transformação genética; Biolística; Eletroporação; Bactéria; Biotecnologia; DNA; Gene; Melhoramento Genético Vegetal; Planta Transgênica.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/562707

Anotação funcional de sequências com BLAST2GO.
Provedor de dados: 14
Autores: NODA, R. W.; DAMASCENO, C. M. B.; SOUSA, S. M. de.
bitstream/item/29525/1/circ-150.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Bioinformática; Gene; Genoma.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/880839

Programa e resumos expandidos.
Provedor de dados: 14
Autores: WORKSHOP DE INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 1., 2004, Brasília, DF.
bitstream/CENARGEN/25860/1/doc123.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Controle de insetos praga; Nematóide de planta.; Gene; Parasitismo.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/186363

Avaliação genômica e resultados para características Slick e Dupla musculatura de fêmeas da raça Senepol: edição 2022.
Provedor de dados: 14
Autores: KATSURAGI, R. R.; NOBRE, P. R. C.; SOUZA JUNIOR, M. D. de; TORRES JUNIOR, R. A. de A.; EGITO, A. A. do; SIQUEIRA, F.; GONDO, A.; MENEZES, G. R. de O.
Dados analisados; Metodologia; Resultados; Outros conceitos importantes; Resultados genômicos para características slick e dupla musculatura; Anexos.
Tipo: Folhetos
Palavras-chave: Gado de Corte; Vaca.; Genética Animal; Gene; Melhoramento Genético Animal.
Ano: 2022
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148597

Síntese da renina humana utilizando o sistema de expressão baculovirus Bac-to- Bac.
Provedor de dados: 14
Autores: MENDES, D. N. M.; SIHLER, W.; NEVES, F. de A. R.; SIMEONI, L. A.; SOUZA, M. L. de S.
bitstream/CENARGEN/29532/1/bp207.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Baculovirus recombinante; Construção; Recombinação homóloga; Co-transfecção de células; VSyn VI-gal; Pré-pró-renina humana.; Inseto; Gene; Vírus.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/189417

Ativação do promotor AtPT2 de Arabidopsis thaliana sob estresse de fósforo em plantas transgênicas de milho.
Provedor de dados: 14
Autores: CARNEIRO, A. A.; CARVALHO, C. H. S.; COELHO, G. T. C. P.; SOUZA, I. R. P.; PAIVA, L. V.; CARNEIRO, N. P.; SCHAFFERT, R. E.; BRANDAO, R. L.; ALVES, V. M. C.
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Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Milho; Zea mays; Gene; Planta Transgênica.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/865599

Biologia sintética.
Provedor de dados: 14
Autores: VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.
A Biologia Sintética, em franca expansão nos dias de hoje, consiste no uso de bioinformática e técnicas de engenharia genética e bioquímica com o objetivo de desenhar circuitos biológicos modulares, por meio do redirecionamento ou construção de novas rotas metabólicas e a criação de organismos artificiais, visando maximizar o seu funcionamento. Não se trata de uma técnica específica, mas de um conceito que pode utilizar diferentes abordagens de edição genômica para alcançar a meta final. Esse documento tem como objetivo fornecer uma visão abrangente desse novo ramo da biologia, de modo a ampliar a nossa percepção sobre as possibilidades futuras que podem ser vislumbradas sobre o desenvolvimento e as aplicações dessa nova abordagem científica.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Genética; Gene; Synthetic biology; Genetics.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1037794

Metodologias de extração e avaliação semi quantitativa da expressão de genes de metabolismo secundário do milho (Zea mays L.).
Provedor de dados: 14
Autores: MESSIAS, R. da S.; GALLI, V.; SILVA, S. D. dos A. e; SCHIRMER, M. A.; PILLON, C. N.
bitstream/item/30587/1/boletim-117.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Gene; Grão; Milho.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/877511

Produção e qualidade de leite de bubalinos no Rio Grande do Sul: dados preliminares.
Provedor de dados: 14
Autores: DAME, M. C. F.; LIMA, C. T. S. de; MARCONDES, C. R.; RIBEIRO, M. E. R.; GARNER, A. D. V.; SANTOS, C. da S. dos.
bitstream/item/44883/1/documento-296.pdf
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Gene; Grão; Milho.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/884503

Avaliação agronômica do milho geneticamente modificado com o gene SbMATE, que confere tolerância ao alumínio: processo de biossegurança para o primeiro estudo de campo.
Provedor de dados: 14
Autores: VASCONCELOS, M. J. V. de; CARNEIRO, A. A.; MAGALHAES, J. V. de; GUIMARÃES, C. T.
O desenvolvimento de uma planta geneticamente modificada envolve a introdução de um ou vários genes exógenos nesse organismo, sem que haja fecundação ou cruzamento. As plantas que tiveram seu código genético alterado pela introdução de uma ou mais sequências de DNA provenientes de outra espécie devem passar por rigorosas análises que comprovam suas características agronômicas e de segurança, tanto para o meio ambiente, como para os seres vivos, cujo conjunto de práticas são chamadas de biossegurança. A biossegurança de uma planta transgênica, além de trabalhos em regime de contenção, passa por liberações planejadas no meio ambiente para se obter dados agronômicos, físico-químicos e ambientais, visando o preparo de um dossiê para a liberação comercial. O...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Biossegurança; Zea Mays; Genética; Gene; Planta Transgênica; Biotecnologia.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1100096

Transformacao de Phaseolus vulgaris Via SAAT ("Sonication-assisted agrobacterium- mediated transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
bitstream/CPAC-2009/24346/1/p2002_72.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transgenico; Transformacao genetica; Transgenics.; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris.; Genetics; Beans; Biotechnology; Genetic transformation; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567648

Bases moleculares e genética da fixação de nitrogênio.
Provedor de dados: 14
Autores: TEIXEIRA, K. R. dos S.
Genes nif e suas funções. Envolvimento de outros genes no processo de FBN Organização estrutural dos genes nif em diversos diazotrófos. Organização dos genes nif em microrganismos não simbióticos. Organização de genes nif em microrganismos simbióticos.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Fixação biológica de nitrogênio; FBN.; Gene; Genética; Microrganismo.
Ano: 1997
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624230

CNV: uma ferramenta promissora para aplicação em programas de melhoramento genético de bovinos.
Provedor de dados: 14
Autores: SIQUEIRA, F.; GIACHETTO, P. F.
Variações no número de cópias de regiões específicas do DNA, ou simplesmente CNVs (Copy Number Variations), constituem um tipo de marcador molecular que é encontrado com frequência nos genomas de mamíferos e plantas. Em humanos, o impacto das CNVs sobre os fenótipos já foi relatado em características adaptativas, mortalidade embrionária e em algumas doenças complexas como autismo, esquizofrenia, doença de Chron, artrite reumatoide, diabetes tipo I e obesidade. Comparada aos SNPs (Single Nucleotide Polymorphism), as CNVs envolvem um número maior de sequências genômicas, apresentando, potencialmente, um impacto mais pronunciado na expressão gênica, em função de alterações na estrutura e na dosagem gênica. Assim, acredita-se que esse marcador também exerça um...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Bovino; DNA; Fenótipo; Gene; Genética Animal; Marcador Molecular; Variação Genética; Melhoramento Genético Animal.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1118279

Engenharia genética: conceitos básicos, ferramentas e aplicações.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132

Introdução de genes em Rhizobium.
Provedor de dados: 14
Autores: SKOT, L.; ARAUJO, R. S.
O objetivo foi a compilação de métodos específicos para estudos de biologia molecular em Rhizobium.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Gene; Nodulação; Rhizobium; Simbiose.
Ano: 1994
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/199957

Validação de marcadores moleculares para eficiência na utilização de fósforo com base em genes SbPSTOL1 em sorgo.
Provedor de dados: 14
Autores: MAGALHAES, J. V.; MACIEL, B. H.; SOUSA, S. M. de; SILVA, L. A.; SCHAFFERT, R. E.; PASTINA, M. M.; BARROS, B. de A.; GUIMARAES, C. T.; NEGRI, B.; AZEVEDO, G.; VIANA, J. H. M.
O presente trabalho teve como objetivo identificar homólogos de sorgo do gene OsPSTOL1, validar o efeito desses genes na absorção de P e na produção de grãos de sorgo cultivado em solos ácidos e gerar marcadores moleculares para seleção assistida com base em genes PSTOL1 em Sorghum bicolor (SbPSTOL1).
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Marcador molecular; Genética; Gene; Sorghum bicolor.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1063400

O gene de tolerância ao alumínio, sbMATE, aumenta ao produção de grãos de sorgo cultivado em solos ácidos.
Provedor de dados: 14
Autores: MAGALHAES, J. V.; CARVALHO JÚNIOR, G. A. de; SCHAFFERT, R. E.; LANDAU, E. C.; MIRANDA, R. A. de; VIANA, J. H. M.; MENEZES, C. B. de; SILVA, L. A.; GUIMARAES, C. T.; COELHO, A. M.
bitstream/item/159487/1/circ-218.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Sorgo; Solo; Toxidez; Alumínio; Gene; Melhoramento genético vegetal.
Ano: 2016
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1063395

Isolamento e caracterização de genes transportadores de fósforo de alta afinidade em sorgo.
Provedor de dados: 14
Autores: VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.; SCHAFFERT, R. E.; RAGHOTHAMA, K. G.
O fosfato (Pi) é um dos macronutrientes essenciais para o crescimento e desenvolvimento das plantas. No entanto, sua disponibilidade é altamente restrita em solos ácidos do Cerrado brasileiro, que são frequentemente usados para o cultivo de sorgo (Sorghum bicolor). Embora vários genótipos contrastantes eficientes e ineficientes do uso de Pi tenham sido obtidos de variantes genéticas naturais, os mecanismos moleculares subjacentes permanecem obscuros. Os transportadores Pi de alta afinidade desempenham um papel fundamental na aquisição de Pi pelas raízes e sua posterior mobilização para partes aéreas. Neste estudo, preparamos uma biblioteca de cDNA a partir de plântulas de sorgo crescidas sob deficiência de Pi que foi escrutinada utilizando-se os...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: SbPTs; Biblioteca de cDNA; Melhoramento Genético Vegetal; Gene; Marcador Genético; Sorghum Bicolor.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112263

Transformacao de feijoeiro com gene Lhcb1*2 por meio do metodo SAAT ("Sonication-assited-agrobacterium transformation").
Provedor de dados: 14
Autores: ANDRADE, S. R. M. de; GOMES, D. G.; LABATE, M. T. V.; LABATE, C. A.
bitstream/CPAC-2009/24345/1/p2002_71.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Transformacao genetica; Transgenico; Transgenics.; Biotecnologia; Feijão; Gene; Melhoramento Genético Vegetal; Phaseolus Vulgaris.; Biotechnology; Genetic transformation; Beans; Plant breeding.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567647

COMBATE ao carrapato com seleção de genes resistentes: programa 01.
Provedor de dados: 14
bitstream/item/100329/1/PGM-05-S-COMBATE-AO-CARRAPATO-COM-SELECAO-DE-GENES-RESISTENTES.mp3
Tipo: Prosa Rural (INFOTECA-E)
Palavras-chave: Seleção genômica; Gado Braford; Gene; Carrapato; Gado de corte; Tristeza parasitária; Sanidade animal; Gado Hereford; Acaricida.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/983602

Genes associados à necrose da cabeça do fêmur em frangos de corte.
Provedor de dados: 14
Autores: PETRY, B.; IBELLI, A. M. G.; PEIXOTO, J. de O.; LEDUR, M. C.
bitstream/item/159864/1/final8527.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E)
Palavras-chave: Melhoramento genético animal; Gene; Frango de corte; Carcaça; Fêmur.
Ano: 2017
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1069596

Avaliação de cultivares de arroz (Oryza sativa L.) para cultivo in vitro.
Provedor de dados: 14
Autores: BEVITORI, R.
Este estudo teve o objetivo de avaliar cinco cultivares elite de arroz quanto ao potencial de produzir calos e regenerar plantas: BRS Bonança, BRS Sertaneja, BRS Esmeralda, BRS Talento e BRS CIRAD 302 (híbrido), que apresentam diferentes características quanto a ciclo, porte, produtividade, dentre outras. A cultivar BRS Bonança foi incluída no estudo para servir de comparação entre as cultivares testadas por apresentar alta capacidade de resposta à indução de calos e regeneração em plantas.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Transformação genética; Regeneração em planta; Arroz; Oryza sativa; Calo; Gene.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1019019

The identification of genes from the oyster Crassostrea gigas that are differentially expressed between progeny exhibiting opposed susceptibility to summer mortality
Provedor de dados: 5
Autores: Huvet, Arnaud; Degremont, Lionel; Labreuche, Yannick; Daniel, Jean-yves; Dubrunfaut, Thomas; Boudry, Pierre; Haure, Joel; Bedier, Edouard; Ropert, Michel; Herpin, Amaury; Cunningham, Charles; Moal, Jeanne; Samain, Jean-francois.
Summer mortality associated with juveniles of the oyster Crassostrea gigas is the result of a complex interaction between the host, pathogens and environment. Divergent selection based on summer survival have been applied to produce families with resistant and susceptible progeny. Here, we describe the use of suppression subtractive hybridization to generate 150 clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these 150 clones was determined. Among the 25 unique sequences that matched with the products of known genes, those putatively implicated in energy generation (12%) and immune function (16%), systems suspected to be implicated in summer mortality, were extended and analyzed by real...
Tipo: Text
Palavras-chave: Selection; Gene; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3447.pdf

Heat shock protein 90 of Bonamia ostreae: characterization and possible correlation with infection of the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Prado Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle.
In this study we described the cytosolic HSP90 of Bonamia ostreae, an intracellular parasite of Ostrea edulis haemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B. ostreae infected haemocytes. HSP90 amplification was corroborated in infected oysters and B. ostreae purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in haemocyte invasion. Our results inform the molecular basis that governs B. ostreae-O. edulis interactions.
Tipo: Text
Palavras-chave: Bivalve haemocytes; Gene; Haplosporidia; Pathogen; Protist; Radicicol.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00137/24799/22878.pdf

Gene expression pattern of digestive and antioxidant enzymes during the larval development of reared Atlantic bluefin tuna (ABFT), Thunnus thynnus L.
Provedor de dados: 5
Autores: Mazurais, David; Coves, Denis; Papandroulakis, Nikos; Ortega, Aurelio; Desbruyeres, Elisabeth; Huelvan, Christine; Le Gall, Marie-madeleine; De La Gandara, Fernando; Cahu, Chantal.
The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and...
Tipo: Text
Palavras-chave: Tuna; Gene; Expression; Development; Digestion; Antioxidant.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00171/28264/26521.pdf

Reduced female gene flow in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie.
The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial...
Tipo: Text
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf

La bonamiose un fléau de l'huître plate....
Provedor de dados: 5
Autores: Morga, Benjamin.
The history of French oyster culture shows very well how fragile this farming is to stock overexploitation and diseases. The production of flat oyster, Ostrea edulis, fell dramatically after the appearance of two parasitic diseases: arteiliosis and bonamiosis. The curative means against bonamiosis (Bonamia ostreae) are restricted and imply a good knowledge of the parasite's life cycle and of its interaction with its host. Studying the expression of the parasite's and the host's genes during the infection seems essential in providing new adapted measures in order to maintain, if not relaunch the flat oyster culture. In this context, this thesis aims to understand the flat oyster-parasite interactions, and especially, the molecular basis of the resistance to...
Tipo: Text
Palavras-chave: Biologie moléculaire; Gene; Parasite; Huîtres; Ostrea edulis.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3369.pdf

Microarray analysis highlights immune response of Pacific oysters as a determinant of resistance to summer mortality
Provedor de dados: 5
Autores: Fleury, Elodie; Huvet, Arnaud.
Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data...
Tipo: Text
Palavras-chave: Mollusca; Crassostrea gigas; Gene; Immunity; CDNA microarray; Summer mortality.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19423/17213.pdf

Development of mussel-specific expression vectors suitable for transgenic organisms: implication on the establishment of mollusc continuous cell lines
Provedor de dados: 5
Autores: Delsert, Claude; Cancela Da Fonseca, Leonor.
The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in...
Tipo: Text
Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi
Provedor de dados: 5
Autores: Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf

Molecular cloning and seasonal expression of oyster glycogen phosphorylase and glycogen synthase genes
Provedor de dados: 5
Autores: Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne.
To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes...
Tipo: Text
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf

Plasmid patterns of efficient and inefficient strains of Bacillus thuringiensis against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 82
Autores: Fagundes,RBS; Picoli,EAT; Lana,UGP; Valicente,FH.
Bacillus thuringiensis harbors genes encoding Cry proteins found in chromosomes or plasmids of different sizes (4-150 Mb). Although the smaller plasmids are more abundant in B. thuringiensis, their specific function is unknown. As for the megaplasmids, their main recognized function is to harbor cry genes, although the sequencing of some of these plasmids indicates the occurrence of other important genes. This work used a new protocol for practical and rapid extraction of plasmid DNA in order to characterize the plasmid patterns of Brazilian strains belonging to Embrapa Milho e Sorgo research center B. thuringiensis bank. We tried to further assess the relationship of plasmid patterns with strains belonging to the same serovars and strains causing 100% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterium; Cry protein; Gene; Megaplasmid.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000500012

Associação de bioensaios e caracterização molecular para seleção de novos isolados de Bacillus thuringiensis efetivos contra Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)
Provedor de dados: 82
Autores: Fatoretto,Júlio C.; Sena,Janete A.D.; Barreto,Marliton R.; Lemos,Manoel V.F.; Boiça Junior,Arlindo L.
A lagarta de Spodoptera frugiperda (J. E. Smith) é uma das principais pragas do milho e para seu controle o Bacillus thuringiensis se destaca por sua atividade entomopatogênica. Este trabalho objetivou a caracterização molecular de isolados de B. thuringiensis quanto à presença do gene cry1 e a avaliação da sua eficiência no controle de lagartas de S. frugiperda. Nas análises da PCR, foi utilizado o Gral-cry1 para confirmação da presença do gene cry1 nos 115 isolados. Uma suspensão de 3 x 10(8) esporos/ml banhou a dieta utilizada para alimentação de 30 lagartas por isolado, com três repetições. A identificação do tipo de genes cry1 dos diferentes isolados foi realizada para cinco sub-classes de genes e análises de regressão linear foram realizadas para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lagarta-do-cartucho; Cry1; Bactéria; Prospecção; Gene.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000500015

Relationship between grain colour and preharvest sprouting-resistance in wheat
Provedor de dados: 86
Autores: Bassoi,Manoel Carlos; Flintham,John.
Since red alleles (R) of the genes that control grain colour are important for the improvement of preharvest sprouting resistance in wheat and there are three independently inherited loci, on chromosomes 3A, 3B and 3D of hexaploid wheat, it is possible to vary the dosage of dominant alleles in a breeding program. The objective of this work was to evaluate the dosage effect of R genes on preharvest sprouting, in a single seed descent population, named TRL, derived from the cross between Timgalen, white-grained wheat, and RL 4137, red-grained wheat. The study was carried out using sprouting data in ripe ears obtained under artificial conditions in a rainfall simulator over three years. According to the results there is a significant effect on preharvest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum; Gene; Allele; Dormancy; Dosage effect.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000006

Phenotypic and genotypic resistance profile of Salmonella Typhimurium to antimicrobials commonly used in poultry
Provedor de dados: 89
Autores: Biffi,CP; Stefani,LM; Miletti,LC; Matiello,CA; Backes,RG; Almeida,JM; Neves,GB.
Isolates of Salmonella sp (104) from poultry samples were isolated and serotyped where eleven were identified as Salmonella Typhimurium (ST). ST isolates were phenotypically tested by disk diffusion and minimum inhibitory concentration (MIC). Four genes related to enrofloxacin (GyrA, GyrB, ParC and ParE), two to gentamicin (AadA and AadB) and two to ceftiofur (BlaCMY-2 and AmpC) resistance were searched by PCR. Our results showed ST resistance to all three antibiotics tested (18.1% for ceftiofur, 45.4% for gentamicin, and 18.1% for enrofloxacin) according to the diffusion test. In the MIC test, the ST isolates showed higher levels of resistance (27.2% for ceftiofur, 54.5% for gentamicin, and 18.2% for enrofloxacin). Three resistance genes out of four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial; Gene; Resistance; Salmonella.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200013

ความหลากหลายของยีนต้านทานไวรัส (Mx1) ในสุกร
Provedor de dados: 106
Autores: Santi Pinthukas; Christoph Knorr; Ratchaneewan Kumphakam; Bertram Brenig; Kesinee Gatphayak.
Disease is a major problem in the swine production. The study of genes associated with disease resistance characteristics is used to select for innate immunity in swine. The Mx1 gene was found to have potent antiviral affect against some virus and can be hereditary. The study of Mx1 polymorphisms in among 3 pig breeds (Thai native pig breeds, Chinese pig breeds and European pig breeds) were found SNPs in exon 8 (c. 1022C&gt;G; 1078A&gt;C) that has allele frequencies of statistical significance between the breeds. (P&lt;0.05) The two variant in exon 15 found silent point mutation (c. 2120G&gt;T) และ 11-bp indels (c.2117_2128 indels CGGCGCCGGCT) that has allele frequencies of statistical significance in some breed (P&lt;0.05)especially in German Landrace and...
Tipo: Collection
Palavras-chave: Porcine; Gene; Mx1; Virus resistance; Porcine myxovirus resistance gene; Genetic marker; สุกร; ยีน; การต้านทานไวรัส; อัลลีล; ความหลากหลายทางพันธุกรรม; ตัวบ่งชี้ทางพันธุกรรม.
Ano: 2011
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5189

การพัฒนาข้าวพันธุ์บางแตนให้ต้านทานเพลี้ยกระโดดสีน้ำตาลโดยใช้เครื่องหมายโมเลกุล
Provedor de dados: 106
Autores: Reunreudee Kaewcheenchai; Sommai Sriwisut; Suradet Palawisut; Apichart Noenplab; Peera Dungsoongnern; Udompan Promnart.
Bahng Taen is a non-glutinous, non-photoperiod sensitive, short duration and high yielding rice variety. This variety showed resistance against brown planthopper (BPH) in the Eastern region, but the resistance has decreased drastically. Therefore, marker-assisted backcrossing was applied to improve BPH resistance by introgression of a Bph3 resistant gene from PTB33  and  Rathu Heenati into Bahng Taen. Two SSR markers, RM 170 for Bahng Taen with PTB33 crossing and RM 190 for Bahng Taen with Rathu Heenati crossing, were used  in combination with plant type selection to obtain the target plants with Bph3 gene. Twenty six and thirty heterozygous BC4F1 plants were developed from the cross of Bahng Taen with PTB33 and Bahng Taen with Rathu Heenati, respectively....
Tipo: PhysicalObject
Palavras-chave: Bahang Taen; Brown planthopper; Back crossing; Rice variety; Marker assisted selection; Insect resistance; Plant breeding; Gene; ข้าว; เพลี้ยกระโดดสีน้ำตาล; การผสมกลับ; การคัดเลือกพันธุ์; พันธุ์บางแตน; เครื่องหมายโมเลกุล; ความต้านทานแมลง; การถ่ายทอดพันธุกรรม; การปรับปรุงพันธุ์; ยีน.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5677

การพัฒนาสายพันธุ์แท้ที่มีศักยภาพสำหรับเป็นแหล่งเชื้อพันธุกรรมในโครงการปรับปรุงพันธุ์ข้าวโพดข้าวเหนียวลูกผสม
Provedor de dados: 106
Autores: Surasak Pidkwamlub; Bunyarit Sinkangam.
The sixty-two of the exotic germplasms waxy corn were performed. The agronomic characters were averaged 45 - 52 days to flowerings, opaque and small kernel. Almost all plant aspects were green and less uniformity, unfortunately, downy mildew was susceptible. Three methods for S1 selection were (1) half - sib (HS) (2) full - sib (FS) and (3) S1 (S1) progeny test. Each method was received for two ears. Also, the agronomic characters were recorded, 45 - 52 days to tasseling and 46 - 52 days to silking. Economic yields, green and white weight were 588 - 1,063 and 340 - 631 kg/rai, namely, UPDW-0013 was showed the best of both. Furthermore, UPCW-0004 showed that the best shelling (71.16%). And, cutting trait was UPWC-0002 (66.67%). Afterward, 26 lines are...
Tipo: PhysicalObject
Palavras-chave: Zea mays; Waxy corn; Plant breeding; Selective breeding; Gene; Inbred line; Hybrid breeding; Crop improvement; ข้าวโพดข้าวเหนียว; พันธุ์ลูกผสม; การปรับปรุงพันธุ์พืช; การคัดเลือกสายพันธุ์; พันธุกรรม; การทดสอบสายพันธุ์; ผลผลิต; แหล่งเชื้อพันธุกรรม.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5739

Mechanisms of plant responses to global climate change
Provedor de dados: 106
Autores: Ed Sarobol.
Tipo: Collection
Palavras-chave: Agriculture; Climate change; Environmental stress; Stress; Hormone; Gene; Plant adaptation; เกษตรกรรม; การตอบสนองของพืช; การปรับปรุงพันธุ์พืช; การเปลี่ยนแปลงสภาพภูมิอากาศโลก; สภาพแวดล้อม; กลไกการทำงานของพืช; ความเครียด; ฮอร์โมน; ยีน; การปรับตัว.
Ano: 2011
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5123

Nuclear entropy, angular second moment, variance and texture correlation of thymus cortical and medullar lymphocytes: Grey level co-occurrence matrix analysis
Provedor de dados: 42
Autores: PANTIC,IGOR; PANTIC,SENKA; PAUNOVIC,JOVANA; PEROVIC,MILAN.
Grey level co-occurrence matrix analysis (GLCM) is a well-known mathematical method for quantification of cell and tissue textural properties, such as homogeneity, complexity and level of disorder. Recently, it was demonstrated that this method is capable of evaluating fine structural changes in nuclear structure that otherwise are undetectable during standard microscopy analysis. In this article, we present the results indicating that entropy, angular second moment, variance, and texture correlation of lymphocyte nuclear structure determined by GLCM method are different in thymus cortex when compared to medulla. A total of 300 thymus lymphocyte nuclei from 10 one-month-old mice were analyzed: 150 nuclei from cortex and 150 nuclei from medullar regions of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Texture analysis; GLCM matrix; CD4; CD8; Gene.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301063

A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
Provedor de dados: 48
Autores: RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article
Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011

Molecular Cloning and Sequencing of AlkalophilicCellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product
Provedor de dados: 52
Autores: Walia,Abhishek; Mehta,Preeti; Guleria,Shiwani; Chauhan,Anjali; Shirkot,C.K.
ABSTRACT A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology withCellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cellulase free xylanase; Cellulosimicrobium cellulans; E.coli; Cloning; Gene.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600913

Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection
Provedor de dados: 62
Autores: Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira.
Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005

Association of the 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene with unstable angina
Provedor de dados: 56
Autores: Iturry-Yamamoto,G.R.; Moriguchi,E.H.; Zago,A.C.; Alho,C.S.; Zago,A.J.
The 894G&gt;T polymorphism of the endothelial constitutive nitric oxide synthase gene consists of the substitution of a guanine base by a thymine at the 894th nucleotide of the gene. An association of this polymorphism with acute coronary syndromes has been described, only when in combination with other polymorphisms of this gene. The aim of the present study was to search for an association between this polymorphism and unstable angina in a southern Brazilian population. In a case-control study, 156 patients (group 1 (N = 83): unstable angina, group 2 (N = 73): stable angina) were genotyped by PCR and digestion of the product. Univariate analysis demonstrated that the minimal luminal diameter and the degree of stenosis of the culprit lesion differed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Gene; Polymorphism; Unstable angina; Coronary.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000400005

Clinical and molecuar characterization of Brazilian patients with growth hormone gene deletions
Provedor de dados: 56
Autores: Arnhold,I.J.P.; Osorio,M.G.F.; Oliveira,S.B.; Estefan,V.; Kamijo,T.; Krishnamani,M.R.S.; Cogan,J.D.; Phillips III,J.A.; Mendonça,B.B.
Genomic DNA from 23 patients with isolated growth hormone (GH) deficiency (12 males and 11 females: heights -4.9 ± 1.4 SDS) was screened for GH gene deletions by restriction endonuclease analysis of polymerase chain reaction amplification products. Three unrelated patients had typical features of severe GH deficiency and deletions (6.7 kb in two and 7.6 kb in one) of the GH gene. The two patients with 6.7-kb deletions developed growth-attenuating anti-GH antibodies whereas the patient with the 7.6-kb deletion continued to grow with GH replacement therapy. Our finding that 3/23 (~13%) Brazilian subjects had GH gene deletions agrees with previous studies of severe isolated GH deficiency subjects in other populations. Two of three subjects (67%) with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth; Growth hormone; Dwarfism; Gene.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400003

Liposomes as a gene delivery system
Provedor de dados: 56
Autores: Ropert,C.
Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle. This has encouraged the development of nonviral DNA-mediated gene transfer techniques such as liposomes. Many liposome-based DNA delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. Another recent technology using cationic lipids has been evaluated and has generated substantial interest in this approach to gene transfer.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene; PH-sensitive liposome; Cationic lipid; Transfection.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200004

Multi-drug resistance gene (MDR1) and opioid analgesia in horses
Provedor de dados: 65
Autores: Natalini,Cláudio Corrêa; Cunha,Anderson Fávaro da; Linardi,Renata Lehn.
Opioid absorption in the intestinal tract as well as its effects in the central nervous system is modulated by the P-glycoprotein (P-gp) encoded in the Multi-drug Resistance gene (MDR1) also named ATP-binding cassete, subfamily B, member 1 (ABCB1). This MDR1 gene acts as a selective pump. The expression of this protein in humans and rodents inhibits cellular uptake of substrate opioids. The presence of the intestinal iso-enzyme CYP3A4 associated with MDR1 gene decreases the opioid analgesic activity due to an increase in intestinal metabolism, with a predicted intestinal first pass extraction around 20% which significantly influences the oral availability of opioids. In the central nervous system, P-gp expression decreases opioid neuronal uptake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1; Analgesia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100055

Identification of multi-drug resistance gene (MDR1) in equine ileum
Provedor de dados: 65
Autores: Natalini,Cláudio Corrêa; Linardi,Renata Lehn.
P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048