Consulta: subjectFacets:"Gene promoter"
Registros recuperados: 3
Data/hora: 01/06/2024 11:24:29

Identification in silico of putative damage responsive elements (DRE) in promoter regions of the yeast genome
Provedor de dados: 74
Autores: Silva,Walkiria Luckwu de Santana; Cavalcanti,Andre Ricardo de Oliveira; Guimarães,Katia Silva; Morais Jr.,Marcos Antonio de.
We report an in silico analysis to identify nucleotide sequence motifs in DNA repair genes that may define a binding site for regulatory proteins during the induction of those genes by mutagens. The damage responsive elements (DRE) weight matrix generated in this analysis was used to search for homologous sequences in the promoter region of all genes, including putative gene and hypothetical open reading frames (ORFs), in the Saccharomyces Genome Data Base (SGD). The results demonstrated that over one third of the yeast genes in the database presented at least one 15-bp sequence in their promoter region with 85% or more of similarity to the DRE consensus sequence. The presence of the DRE sequence in the promoter region of regulatory genes and its high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA binding site; DNA repair; Gene promoter; Gene regulation; Transfac; Weight matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500025

A pilot study on genetic variation in purine-rich elements in the nephrin gene promoter in type 2 diabetic patients
Provedor de dados: 48
Autores: GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA.
Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter...
Tipo: Journal article
Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010

Essential regulatory elements for NHE3 gene transcription in renal proximal tubule cells
Provedor de dados: 56
Autores: Neri,E.A.; Bezerra,C.N.A.; Rebouças,N.A.
The objectives of the present study were to identify the cis-elements of the promoter absolutely required for the efficient rat NHE3 gene transcription and to locate positive and negative regulatory elements in the 5’-flanking sequence (5’FS), which might modulate the gene expression in proximal tubules, and to compare this result to those reported for intestinal cell lines. We analyzed the promoter activity of different 5’FS segments of the rat NHE3 gene, in the OKP renal proximal tubule cell line by measuring the activity of the reporter gene luciferase. Because the segment spanning the first 157 bp of 5’FS was the most active it was studied in more detail by sequential deletions, point mutations, and gel shift assays. The essential elements for gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Na+/H+ exchanger isoform 3; Gene promoter; Specificity protein 1; Early growth response factor 1; Proximal tubules.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600004