Consulta: subjectFacets:"Genetic characterization"
Registros recuperados: 7
Data/hora: 09/06/2024 07:35:57

Microsatellite characterization of Cimarron Uruguayo dogs
Provedor de dados: 74
Autores: Gagliardi,Rosa; Silvia,Llambí; García,Cristina; Arruga,María Victoria.
Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values &gt; 0.7, in agreement with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cimarron Uruguayo dog; Genetic characterization; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028

D-loop haplotype diversity in Brazilian horse breeds
Provedor de dados: 74
Autores: Ianella,Patrícia; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Egito,Andréa Alves do; Almeida,Leonardo Daniel; Sereno,Fabiana T. P. S.; Carvalho,Luiz Felipe Ramos; Mariante,Arthur da Silva; McManus,Concepta Margaret.
Abstract The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mitochondrial DNA; Genetic characterization; Equus caballus; Locally adapted breeds; Animal genetic resources.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400604

Molecular Characterization of Novel Cryptosporidium Fish Genotypes in Edible Marine Fish
Provedor de dados: 5
Autores: Certad, Gabriela; Zahedi, Alireza; Gantois, Nausicaa; Sawant, Manasi; Creusy, Colette; Duval, Erika; Benamrouz-vanneste, Sadia; Ryan, Una; Viscogliosi, Eric.
Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be...
Tipo: Text
Palavras-chave: Piscine Cryptosporidium; Edible marine fish; Genetic characterization; 18S rDNA gene; Actin gene; Molecular phylogeny.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00665/77741/79864.pdf

Mitochondrial Genetic Characterization Of Bluefin Tuna (Thunnus Thynnus) From Three Mediterranean (Libya, Malta, Tunisia); And One Atlantic Locations (Gulf Of Cadiz).
Provedor de dados: 20
Autores: Farrugia, A.F.; Pla, C.; Viñas, J.; Tawil, M.Y.; Hattour, A.; de la Serna, J.M.
None of the previous genetic analysis found significant differences within the Mediterranean. However, a more exhaustive analysis is needed inside this sea. In this sense, we have analyzed the mitochondrial control region sequence variability of three Mediterranean locations: Libya (n = 22), Tunisia (n = 23) and Malta (n = 12). Additionally a sample from the Gulf of Cadiz (n = 24) in the East Atlantic Ocean was also included in the study. The AMOVA analysis revealed slightly (Fst = 0.013) but significative genetic differences (P &lt; 0.000) for the Malta location, probably due to its small sample size (n = 12). In the other hand, when this location is removed from the analysis, no differences where found among the three remaining location (Fst = 0,005; P =...
Tipo: Journal Contribution
Palavras-chave: Atlantic ocean; Mitochondrial DNA; Bluefin tuna; Sequencing; Genetic characterization; Mediterranean sea; Tuna fisheries.
Ano: 2003
URL: http://hdl.handle.net/1834/792

Genetic diversity and population structure of Brazilian native bovine breeds
Provedor de dados: 86
Autores: Serrano,Germana Maria; Egito,Andréa Alves do; McManus,Concepta; Mariante,Arthur da Silva.
Conservation and improvement strategies should be based on the association between genetic and phenotypic characteristics. The objective of this work was to characterize five native Brazilian cattle breeds (Caracu, Crioulo Lageano, Curraleiro, National Polled and Pantaneiro) and two commercial breeds (Holstein and Nellore) using RAPD technique to estimate genetic distances and variability between and within breeds. Genetic relationships were investigated using 22 primers which generated 122 polymorphic bands. Analysis of molecular variance indicated that most of the genetic variation lay among individuals within populations. The genetic variabilities between pairs of breeds were statistically significant. The smallest genetic divergence was between Crioulo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal conservation; Cattle; Genetic characterization; RAPD.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600005

Identificarion of contaminant bacteria in cachaça yeast by 16s rDNA gene sequencing
Provedor de dados: 63
Autores: Carvalho-Netto,Osmar Vaz de; Rosa,Daniel Dias; Camargo,Luis Eduardo Aranha.
Cachaça is a typical Brazilian liquor produced from the distillation of fermented sugarcane juice mainly by Saccharomyces cerevisiae. Most of the domestic production is artisanal, and producers usually are not concerned regarding microbiological control of the fermentation. This study aimed to characterize the contaminant bacterial community of the yeast used in the production of cachaça in an artisanal still. Four samples were collected, of which one (NA) was used for comparison purposes and was collected one year earlier. The remaining samples were collected at three different periods: at the end of the first day of fermentation (NP), after fifteen days (NS), and thirty days after the same yeast was used (NT). Five hundred and eighty-seven sequences were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aguardente; Alcoholic fermentation; Genetic characterization; Pé-de-cuba.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500010

Genetic characterization of a brangus-ibage cattle population: biochemical polymorphisms and reproductive efficiency
Provedor de dados: 65
Autores: Henkes,Luiz Ernani; Papadopolis,Lídia Gonzalez; Steigleder,Clara Sabina; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
Biochemical techniques were used to investigate the genetic variability in a Brangus-Ibage population by determining allele frequencies of 18 blood protein systems: Hemogloin beta-Chain (Hb), Albumin (Alb), Amylase (Am), Transferrin (Tf), Carbonic Anhydrase (CA), Ceruloplasmin (Cp), Malic Enzyme (ME), Diaphorase I and II (Dia I and Dia II), Slow Alpha 2 Macroglobulin (Ap), Acid Phosphatase (ACP), Esterase B and D (EstB and EstD), Phosphogluconate Dehydrogenase (PGD), Glucose-6-Phosphate Dehydrogenase (G-6-PD), Glucose-Phosphate-Isomerase (GPI), Superoxide Dismutase (SOD) and Glyoxalase I (GLO). The percentage of polymorphic loci were estimated at 0.27, the mean number of alleles was 1.33 and the mean heterozygosity was 0.07. There was a good agreement...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brangus-Ibage cattle; Genetic characterization; Reproductive efficiency.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000500010