Consulta: subjectFacets:"Germline cells"
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Data/hora: 10/06/2024 18:31:49

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers
Provedor de dados: 5
Autores: Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude.
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster...
Tipo: Text
Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf