Consulta: subjectFacets:"GlnB"
Registros recuperados: 2
Data/hora: 03/06/2024 09:55:45

Partial characterization of nif genes from the bacterium Azospirillum amazonense
Provedor de dados: 56
Autores: Potrich,D.P.; Passaglia,L.M.P.; Schrank,I.S.
Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum amazonense; Nif genes; GlnB; 16S rDNA.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900002

Different responses of the GlnB and GlnZ proteins upon in vitro uridylylation by the Azospirillum brasilense GlnD protein
Provedor de dados: 56
Autores: Araújo,L.M.; Huergo,L.F.; Invitti,A.L.; Gimenes,C.I.; Bonatto,A.C.; Monteiro,R.A.; Souza,E.M.; Pedrosa,F.O.; Chubatsu,L.S.
Azospirillum brasilense is a diazotroph found in association with important agricultural crops. In this organism, the regulation of nitrogen fixation by ammonium ions involves several proteins including the uridylyltransferase/uridylyl-removing enzyme, GlnD, which reversibly uridylylates the two PII proteins, GlnB and GlnZ, in response to the concentration of ammonium ions. In the present study, the uridylylation/deuridylylation cycle of A. brasilense GlnB and GlnZ proteins by GlnD was reconstituted in vitro using the purified proteins. The uridylylation assay was analyzed using non-denaturing polyacrylamide gel electrophoresis and fluorescent protein detection. Our results show that the purified A. brasilense GlnB and GlnZ proteins were uridylylated by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Azospirillum brasilense; Nitrogen fixation; PII-like protein; GlnD; GlnB; GlnZ.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400006