Consulta: subjectFacets:"Glutaraldehyde"
Registros recuperados: 5
Data/hora: 06/06/2024 02:19:42

Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Provedor de dados: 69
Autores: Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán.
The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 Â± 21 U/g of CLEA and 4800 Â± 30 U/g of CLEA and particle sizes between 52.6 Â± 18.7 Âµm and 126.2 Â± 53.5 Âµm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 Â± 16 U/g of CLEA and 2874 Â± 20 U/g of CLEA and particle sizes between 21.2 Â± 5.1 Âµm and 83.4 Â± 24.9 Âµm); Thermal stability assays of bioctalysts at 60ÂºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual...
Tipo: Journal article
Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010

An Improved Protocol for Flow Cytometry Analysis of Phytoplankton Cultures and Natural Samples
Provedor de dados: 5
Autores: Marie, Dominique; Rigaut-jalabert, Fabienne; Vaulot, Daniel.
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of side scatter (a proxy of cell size) and fluorescence of natural pigments. We found that different marine phytoplankton species react differently to the action of Pluronic F68. In particular, photosynthetic prokaryotes are less sensitive than eukaryotes. Observed cell losses may result from cell lysis or from cell adhesion to the walls of plastic tubes that are commonly used for flow cytometry analysis....
Tipo: Text
Palavras-chave: Phytoplankton; Flow cytometry; Preservation; Pluronic F68; Glutaraldehyde.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00374/48493/48858.pdf

Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
Provedor de dados: 77
Autores: Guerrero,F; Cerutti,P; Marcaccini,A.
Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of the sample processing. Therefore in this work, the quality of preservation of haemal nodes fixed with two classic aldehyde fixatives: formaldehyde and glutaraldehyde we have compared under the scanning electron microscope. Our results showed that both fixatives were successful in preserving the morphology of haemal nodes components; however glutaraldehyde conferred satisfactory results mainly in the preservation of parenchymal cells, whereas formaldehyde was...
Tipo: Journal article
Palavras-chave: Scanning electron microscopy; Fixation; Fixative; Haemal node; Glutaraldehyde; Formaldehyde.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059

Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems
Provedor de dados: 52
Autores: Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed.
ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447

The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA
Provedor de dados: 56
Autores: Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013