Consulta: subjectFacets:"HCV"
Registros recuperados: 31
Data/hora: 01/06/2024 06:09:30

Association of killer cell immunoglobulin-like receptor polymorphisms with chronic hepatitis C and responses to therapy in Brazil
Provedor de dados: 74
Autores: Vasconcelos,Janaina Mota de; Móia,Lizomar de Jesus Maués Pereira; Amaral,Ivanete do Socorro Abraçado; Miranda,Esther Castello Branco Mello; CicaliseTakeshita,Louise Yukari; Oliveira,Layanna Freitas de; Mendes,Lilian de Araújo Melo; Sastre,Danuta; Tamegão-Lopes,Bruna Pedroso; Pedroza,Larysse Santa Rosa de Aquino; Santos,Sidney Emanuel Batista dos; Soares,Manoel do Carmo Pereira; Araújo,Marialva Tereza Ferreira de; Bandeira,Camila Lucas; Silva,Adriana Maria Paixão de Sousa da; Medeiros,Zilene Lameira de; Sena,Leonardo; Demachki,Samia; Santos,Eduardo José Melo dos.
Soroprevalence for Hepatitis C virus is reported as 2.12% in Northern Brazil, with about 50% of the patients exhibiting a sustained virological response (SVR). Aiming to associate polymorphisms in Killer Cell Immunoglobulin-like Receptors (KIR) with chronic hepatitis C and therapy responses we investigated 125 chronic patients and 345 controls. Additionally, 48 ancestry markers were genotyped to control for population stratification. The frequency of the KIR2DL2 and KIR2DL2+HLA-C Asp80 gene and ligand was higher in chronic infected patients than in controls (p &lt; 0.0009, OR = 3.4; p = 0.001, OR = 3.45). In fact, KIR2DL3 is a weaker inhibitor of NK activity than KIR2DL2, which could explain the association of KIR2DL2 with chronic infection. Moreover,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; KIR; HLA-C; Hepatitis C; KIR2DL2.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100004

Levels of interleukins 12 (IL-12) and 13 (IL-13), hepatitis B and C serology, and blood cultures among acute myeloid leukemia (AML) patients in Egypt
Provedor de dados: 80
Autores: RAA,Nour el deen; RA,Harfoush; MM,Elgharabawy; NA,Hamed; MG,Morsi.
There is an interest in the use of IL-12 as a possible anti-cancer drug to induce immune responses and anti-IL-13 formulations to treat the undesirable effects of IL-13. Thus, the present study aimed at analyzing IL-12 and IL-13 profiles, viral hepatitis serology and blood cultures in acute myeloid leukemia (AML) patients. Forty individuals (20 without septicemia - Group A, and 20 with septicemia - Group B) and 20 healthy controls were evaluated. Hepatitis B virus antigens (HBsAg) and hepatitis C virus antibodies (HCV Ab) were quantified using commercial ELISA kits. IL-12 and IL-13 levels were estimated in culture supernatant of mitogen-stimulated peripheral blood mononuclear cells by ELISA. Significantly low IL-12 values were observed among AML patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myeloid leukemia; IL-12; IL-13; HBsAg; HCV; ELISA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300009

LACK OF EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN HUMAN MONOCYTE-DERIVED DENDRITIC CELLS USING RECOMBINANT SEMLIKI FOREST VIRUS
Provedor de dados: 37
Autores: NAVAS,Maria-Cristina; STOLL-KELLER,Françoise; PAVLOVIC,Jovan.
ABSTRACT Hepatitis C Virus belongs to the Flaviviridae family. One proposed mechanism of HCV persistence in the ability to infect hematopoietic cells, including Dendritic cells (DCs). HCV infection of DCs could impair their functions that represent one of the mechanisms, thus hampering viral clearance by the host immune system. Among HCV-encoded proteins, the highly conserved Core protein has been suggested to be responsible for the immunomodulatory properties of this Hepacivirus. Recombinant viral vectors expressing the HCV Core protein and allowing its transduction and therefore the expression of the protein into DCs could be useful tools for the analysis of the properties of the Core protein. Vaccinia Virus and retrovirus have been used to transduce...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendritic cells; HCV; SFV; Viral vector.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300493

Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
Provedor de dados: 48
Autores: ACOSTA-RIVERO,NELSON; POUTOU,JOANNA; ÁLVAREZ-LAJONCHERE,LIZ; GUERRA,IVIS; AGUILERA,YARAIMA; MUSACCHIO,ALEXIS; RODRÍGUEZ,ARMANDO; AGUILAR,JULIO C; FALCON,VIVIANA; ÁLVAREZ-OBREGON,JULIO C; SORIA,YORDANKA; TORRES,DINORAH; LINARES,MARBELIS; PÉREZ,ÁNGEL; MORALES-GRILLO,JUAN; DUEÑAS -CARRERA,SANTIAGO.
In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular immune responses. Particularly, HCcAg.120-VLPs induced specific delayed type hypersensitivity, and generated a predominant T helper 1 cytokine pro file in immunized mice. In addition, HCcAg.120-VLPs prime splenocytes proliferate in vitro against different HCcAg.120-specific peptides, depending on either the immunization route or the adjuvant used. Remarkably, immunization with HCcAg.120-VLPs/Montanide ISA888 formulation resulted in a significant control of...
Tipo: Journal article
Palavras-chave: Challenge; HCV; Immunity; Adjuvant; Capsid.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100005

Hematological particularities and co-infections in injected drug users with AIDS
Provedor de dados: 55
Autores: Brunetta,Denise Menezes; De Santis,Gil Cunha; Vilar,Fernando Crivelenti; Brandão,Renata Amorim; Muniz,Renata Zomer de Albernaz; Lima,Geovana Momo Nogueira de; Amorelli-Chacel,Manuela Emiliana; Covas,Dimas Tadeu; Machado,Alcyone Artioli.
HIV patients infected through injected drug use have poorer prognosis than other groups. We evaluated the hematological alterations and rates of co-infections in injected drug use patients with AIDS. Injected drug use patients were younger, predominantly of male gender, and presented lower CD4, total lymphocyte, and platelet counts, but not neutrophil count, than control group. Injected drug use patients had a higher rate of hepatitis C and mycobacteria infection. Furthermore, all injected drug use patients with hemoglobin &lt;10.0 g dL-1 and lymphocyte &lt;1000 µL-1 had CD4 count lower than 100 µL-1. In conclusion, HIV-infected injected drug use patients constitute a special group of patients, and hemoglobin concentration and lymphocyte count can be used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AIDS; Injected drug use; Anemia; HCV; Mycobacteria.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600005

Characteristics of co-infections by HCV and HBV among Brazilian patients infected by HIV-1 and/or HTLV-1
Provedor de dados: 55
Autores: Moreira,Marcia; Ramos,André; Netto,Eduardo M.; Brites,Carlos.
BACKGROUND: The human retroviruses HIV-1 and HTLV-1 share the routes of infection with hepatitis viruses B and C. Co-infection by these agents are a common event, but we have scarce knowledge on co-infection by two or more of these agents. OBJECTIVE: To evaluate the characteristics and risk factors for co-infections by HBV and HCV in patients infected by HIV-1 or/and HTLV-1, in Salvador, Brazil. METHODS: In a case-control study we evaluated patients followed in the AIDS and HTLV clinics of Federal University of Bahia Hospital. Clinical and epidemiological characteristics were reviewed, and patients were tested for the presence of serological markers of HBV and HCV infections. HCV-infected patients were tested by PCR to evaluate the presence of viremia....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; HTLV-1; Coinfection; HCV; HBV; Brazil.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600007

How are HCV-infected patients being identified in Brazil: a multicenter study
Provedor de dados: 55
Autores: Portari-Filho,Luiz H.; Álvares-da-Silva,Mario R.; Gonzalez,Aline; Ferreira,Adalgisa P.; Nogueira,Cristiane V.; Mendes-Correa,Maria C.; Lima,José M.; Lopes,Edmundo P.; Brandão,Carlos E.; Ivantes,Cláudia; Lyra,André; Lindenberg,Andreia; Ferraz,Maria L.
ABSTRACT  Background: Hepatitis C is an important health problem. In Brazil, 1-2 million people are infected. Despite this expressive number, and the availability of very successful treatment, many patients remained undiagnosed mainly because of the asymptomatic nature of the infection.  Objectives: To describe epidemiological characteristics of HCV-infected patients seen at referral centers in Brazil, the source of referral, and the time spanned to reach a reference center, in order to improve the identification of undiagnosed patients.  Methods: Multicenter observational, cross-sectional study carried out in 15 centers of Brazil, between January/2016 and June/2017. Data of patients with a confirmed diagnosis (anti-HCV and HCV-RNA) were collected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epidemiology; Hepatitis C; HCV; Brazil.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100034

Magnetic bead technology for viral RNA extraction from serum in blood bank screening
Provedor de dados: 55
Autores: Albertoni,Guilherme Ambrozio; Arnoni,Carine Prisco; Araujo,Patricia Regina Barboza; Andrade,Sheila Siqueira; Carvalho,Fabrício Oliveira; Girão,Manoel João Batista Castello; Schor,Nestor; Barreto,José Augusto.
Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; HIV; Infection; Reverse transcriptase polymerase chain reaction; Blood banks.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008

One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil
Provedor de dados: 55
Autores: Andrade,Elisabete; Rocha,Daniele; Fontana-Maurell,Marcela; Costa,Elaine; Ribeiro,Marisa; de Godoy,Daniela Tupy; Ferreira,Antonio G.P.; Tanuri,Amilcar; Alvarez,Patrícia; Brindeiro,Rodrigo.
ABSTRACT The Brazilian Public Health Service provides freely αPEG-IFN to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects the treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay and it has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; Viral load; Real Time PCR; Monitoring patients.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500418

High prevalence of hepatitis C infection in a Brazilian prison: identification of risk factors for infection
Provedor de dados: 55
Autores: Guimarães,Thaís; Granato,Celso F. H.; Varella,Drauzio; Ferraz,Maria Lúcia G.; Castelo,Adauto; Kallás,Esper Georges.
Hepatitis C virus (HCV) causes infectious hepatitis worldwide. It is transmitted mainly by blood products and sharing of intravenous paraphernalia during illicit drug use. High prevalence rates have been described among specific groups considered to be at higher risk for HCV infection, including prison inmates. The objectives of this study were: to determine the HCV seroprevalence among inmates of Casa de Detenção de São Paulo; to identify risk factors for HCV infection; and to compare the seroprevalence of HCV to other blood borne or sexually transmitted diseases. From December, 1993, to January, 1994, a total of 779 inmates were interviewed to collect information on sociodemographic status, sexual behavior, and past experience with illicit drugs. Blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; Seroprevalence; Prison; Risk factor.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000300002

Prevalence and characteristics of HIV/HBV and HIV/HCV coinfections in Tuscany
Provedor de dados: 55
Autores: Puglia,Monia; Stasi,Cristina; Da Frè,Monica; Voller,Fabio.
Abstract  Introduction Worldwide about 30% of HIV-infected patients are coinfected with HCV or HBV. The HIV/HCV coinfection is more common in individuals who have a history of drug addiction. The aims of this study were to assess the HCV and HBV prevalence in HIV-infected patients and analyze their characteristics.  Methods We considered the new HIV diagnoses notified by the regional surveillance system of Tuscany from 2009 to 2013. Descriptive analyses were conducted on the socio-demographic characteristics, routes of transmission, and reason to perform the test. In coinfected patients we assessed the risk for being late presenter (LP) or the risk of having AIDS.  Results In 5 years of surveillance a total of 1354 new HIV diagnoses were notified: 1188...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; HBV; HCV; Coinfections.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400330

HBV, HCV and HIV seroprevalence among blood donors in Istanbul, Turkey: how effective are the changes in the national blood transfusion policies?
Provedor de dados: 55
Autores: Acar,Ali; Kemahli,Sabri; Altunay,Husnu; Kosan,Erdogan; Oncul,Oral; Gorenek,Levent; Cavuslu,Saban.
The national blood transfusion policies have been changed significantly in recent years in Turkey. The purpose of this study was to determine the prevalence of HBV, HCV, and HIV in blood donors at the Red Crescent Center in Istanbul and to evaluate the effect of changes in the national blood transfusion policies on the prevalence of these infections. The screening results of 72695 blood donations at the Red Crescent Center in Istanbul between January and December 2007 were evaluated retrospectively. HBsAg, anti-HCV, and anti-HIV-1/2 were screened by microparticle enzyme immunoassay (MEIA) method. Samples found to be positive for anti-HIV 1/2 and anti-HCV were confirmed by Inno-Lia HCV Ab III and Inno-Lia HIV I/II Score, respectively. The seropositivity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HCV; HIV; Seroprevalence; Blood donors; Turkey.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100009

Impact of psychiatric disorders on the quality of life of brazilian HCV-infected patients
Provedor de dados: 55
Autores: Batista-Neves,Susana; Quarantini,Lucas C.; Galvão-de Almeida,Amanda; Cardeal,Maurício; Lacerda,Acioly L.; Paraná,Raymundo; Reis de-Oliveira,Irismar; Bressan,Rodrigo A.; Miranda-Scippa,Angela.
The aim of our study was to determine the impact of psychiatric comorbidities on the health-related quality of life of HCV-infected patients. Assessment of clinical, socio-demographic and quality of life data of the patients followed up at a Hepatology unit was performed by using a standard questionnaire and the SF-36 instrument. Psychiatric diagnoses were confirmed by using the Mini International Neuropsychiatric Interview, Brazilian version 5.0.0 (MINI Plus). Evaluation using the MINI plus demonstrated that 46 (51%) patients did not have any psychiatric diagnosis, while 44 (49%) had at least one psychiatric diagnosis. Among patients with a psychiatric comorbidity, 26 (59.1%) had a current mental disorder, out of which 22 (84.6%) had not been previously...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mental disorders; Quality of life; HCV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100009

Demographic and anthropometrical analysis and genotype distribution of chronic hepatitis C patients treated in public and private reference centers in Brazil
Provedor de dados: 55
Autores: Focaccia,R.; Baraldo,D.C.M.; Ferraz,M.L.G.; Martinelli,A.L.C.; Carrilho,F.J.; Gonçales Jr.,F.L.; Pedroso,M.L.A.; Coelho,H.S.M.; Lacerda,M.A.; Brandão,C.E.; Mattos,A.A.; Lira,L.G.C.; Zamin Jr.,I.; Pinheiro,J.O.P.; Tovo,C.V.; Both,C.T.; Soares,J.A.S.; Dittrich,S.
Hepatitis C virus (HCV) infection is a serious public health problem, since 80% to 85% of HCV carriers develop a persistent infection that can progress into liver cirrhosis and hepatocarcinoma. Considering that the response of hepatitis C patients to combination therapy with interferon and ribavirin depends on HCV characteristics as well as on host features, we made a retrospective analysis of demographic and anthropometrical data and HCV genotype distribution of chronic hepatitis C patients treated in public and private reference centers in Brazil. The medical records of 4,996 patients were reviewed, 81% from public and 19% from private institutions. Patients' median age was 46 years, and there was a higher prevalence of male (62%) and white patients...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; Chronic hepatitis C; Genotype; Body weight; Brazil.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500003

Seroprevalence of hepatitis B and hepatitis C markers among children and adolescents in the south brazilian region: metropolitan area of Florianópolis, Santa Catarina
Provedor de dados: 55
Autores: Voigt,Andréia Royer; Strazer Neto,Miguel; Spada,Celso; Treitinger,Arício.
Hepatitis B and C are important causes of morbidity and mortality worldwide. In Brazil, according to the Ministry of Health, about 15% of population is infected by hepatitis B virus (HBV) and less than 1% by hepatitis C virus (HCV). Nevertheless, the age-specific prevalence of HBV and HCV markers remains unknown. This study aimed to determine the seroprevalence of HBV and HCV markers of infection and immunity in children and adolescents between 10 to 16 years old who live in the metropolitan area of Florianópolis, state of Santa Catarina, South of Brazil. Three hundred and eighty four individuals were enrolled in this study. Serological markers for HBV and HCV (HBsAg, total anti-HBc, anti-HBc IgM, anti-HBs and anti-HCV) were determined through...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HCV; HBsAg; Anti-HBs; Anti-HBc; Anti-HCV; Seroprevalence.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100012

Long-term follow-up of patients with chronic hepatitis C with sustained virologic response to interferon
Provedor de dados: 55
Autores: Ferreira,Sandro da Costa; Carneiro,Marcos de Vasconcelos; Souza,Fernanda Fernandes; Teixeira,Andreza Corrêa; Villanova,Marcia Guimarães; Figueiredo,José Fernando de Castro; Passos,Afonso Dinis Costa; Ramalho,Leandra Naira Zambelli; Zucoloto,Sergio; Martinelli,Ana de Lourdes Candolo.
BACKGROUND AND AIM: The durability of the sustained virologic response (SVR) in patients with chronic hepatitis C after treatment and the ideal follow-up time for these patients remains undefined. The objective of the study was to evaluate the durability of the virologic response in patients with chronic hepatitis C followed up for at least 12 months after SVR at HCFMRP-USP. METHODS: The study was conducted on 174 patients with chronic hepatitis C treated with different antiviral regimens who had achieved SVR. Qualitative serum HCV-RNA was determined by the commercial kit (COBAS AMPLICOR HCV, v2.0). RESULTS: There was predominance of male (73%) with a mean age of 45.6 ± 10 years. Liver cirrhosis was present in 16.1% of the study subjects. Mean follow-up...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis C; Interferon; HCV; Follow-up; Hepatocellular carcinoma.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400003

Disease burden of chronic hepatitis C in Brazil
Provedor de dados: 55
Autores: Ferreira,Paulo Roberto Abrão; Brandão-Mello,Carlos Eduardo; Estes,Chris; Gonçales Júnior,Fernando Lopes; Coelho,Henrique Sérgio Moraes; Razavi,Homie; Cheinquer,Hugo; Wolff,Fernando Herz; Ferraz,Maria Lúcia Gomes; Pessoa,Mário Guimarães; Mendes-Correa,Maria Cássia.
Background: Hepatitis C virus infection is a major cause of cirrhosis; hepatocellular carcinoma; and liver transplantation. The aim of this study was to estimate hepatitis C virus disease progression and the burden of disease from a nationwide perspective.Methods: Using a model developed to forecast hepatitis C virus disease progression and the number of cases at each stage of liver disease; hepatitis C virus-infected population and associated disease progression in Brazil were quantiﬁed. The impact of two different strategies was compared: higher sustained virological response and treatment eligibility rates (1) or higher diagnosis and treatment rates associated with increased sustained virological response rates (2).Results: The number of infected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; Disease burden; Epidemiology; Incidence; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400363

In vitro basal T-cell proliferation among asymptomatic Human T cell Leukemia Virus type 1 patients co-infected with hepatitis C and/or Human Immunodeficiency Virus type 1
Provedor de dados: 55
Autores: Assone,Tatiane; Kanashiro,Tatiana M.; Baldassin,Maira P.M.; Paiva,Arthur; Haziot,Michel E.; Smid,Jerusa; Oliveira,Augusto Penalva de; Fonseca,Luiz Augusto M.; Norris,Philip J.; Casseb,Jorge.
ABSTRACT  Background: Infection with Human T cell Leukemia Virus type 1 can be associated with myelopathy/tropical spastic paraparesis (HAM/TSP) and other inflammatory diseases. Lymphocytes from about half of Human T cell Leukemia Virus type 1-infected subjects spontaneously proliferate in vitro, and how this phenomenon relates to symptomatic disease and viral burden is poorly understood.  Objective: To evaluate T-cell proliferation in vitro among patients co-infected with Human T cell Leukemia Virus type 1/Hepatitis C Virus/Human Immunodeficiency Virus type 1.  Material and methods: From 610 Human T cell Leukemia Virus-infected patients of the Human T cell Leukemia Virus outpatient clinic from Institute of Infectious Diseases “Emilio Ribas” in São Paulo,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; HTLV-1; HIV-1; T-cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200106

Phylogenetic analysis of the emergence of main hepatitis C virus subtypes in São Paulo, Brazil
Provedor de dados: 55
Autores: Nishiya,Anna Shoko; Almeida-Neto,César de; Romano,Camila Malta; Alencar,Cecília Salete; Ferreira,Suzete Cleusa; Di-Lorenzo-Oliveira,Claudia; Levi,José Eduardo; Salles,Nanci Alves; Mendrone-Junior,Alfredo; Sabino,Ester Cerdeira.
ABSTRACTBACKGROUND: It is recognized that hepatitis C virus subtypes (1a, 1b, 2a, 2b, 2c and 3a) originated in Africa and Asia and spread worldwide exponentially during the Second World War (1940) through the transfusion of contaminated blood products, invasive medical and dental procedures, and intravenous drug use. The entry of hepatitis C virus subtypes into different regions occurred at distinct times, presenting exponential growth rates of larger or smaller spread. Our study estimated the growth and spread of the most prevalent subtypes currently circulating in São Paulo.METHODS:A total of 465 non-structural region 5B sequences of hepatitis C virus covering a 14-year time-span were used to reconstruct the population history and estimate the population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth rate; HCV; Phylogenetic analysis; Subtypes.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500473

Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship?
Provedor de dados: 55
Autores: Albertoni,Guilherme; Arnoni,CP; Araújo,PRB; Carvalho,FO; Barreto,JA.
BACKGROUND: Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as ''reactive'' or ''non reactive'' and EIA S/CO ratio may also be reported as ''high'' or ''low.'' This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. STUDY DESIGN AND METHODS: Sera from blood donors were analyzed by Enzyme-Linked Immunosorbent Assay (ELISA) and RT-PCR assay to detect HCV infection. RESULTS: The RT-PCR assay to genotypes 1a/b showed an acceptable linear response in serial dilutions. The samples were divided into two groups based on their serological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; HCV genotype 1; Real-time PCR; ELISA; RNA extraction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200006

Hepatitis C virus detection in the semen of infected patients
Provedor de dados: 55
Autores: Cavalheiro,Norma de Paula; Santos,Ana Carolina de Oliveira; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Barone,Antonio Alci.
Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche) and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%), blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; PCR; Sexual transmission; Semen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500003

Hepatitis C viral load in HCV-monoinfected and HCV/HIV-1-, HCV/HTLV-1/-2-, and HCV/HIV/HTLV-1/-2-co-infected patients from São Paulo, Brazil
Provedor de dados: 55
Autores: Alves,Fabiana Aparecida; Campos,Karoline Rodrigues; Lemos,Marcílio Figueiredo; Moreira,Regina Célia; Caterino-de-Araujo,Adele.
ABSTRACT Co-infections of hepatitis C virus (HCV) and either human immunodeficiency virus type 1 (HIV-1), human T-cell lymphotropic virus type 1 (HTLV-1) or type 2 (HTLV-2) have been described as having an impact on HCV viremia and subsequent disease progression. HCV load in serum samples from 622 patients (343 males, 279 females; median age 50.8 years) from São Paulo/southeast Brazil was analyzed using the Abbott Real Time HCV assay (Abbott Molecular Inc., IL, USA). Samples were obtained from HCV-monoinfected (n = 548), HCV/HIV-1- (n = 41), HCV/HTLV-1- (n = 16), HCV/HTLV-2- (n = 8), HCV/HIV/HTLV-1- (n = 4), and HCV/HIV/HTLV-2-co-infected (n = 5) patients, and results were compared among the groups and according to sex. The median HCV load in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; HIV-1; HTLV-1: HTLV-2; Co-infection; Viral load.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200123

Hepatitis C treatment: shorter and better?
Provedor de dados: 55
Autores: Araújo,Evaldo Stanislau Affonso de; Courtouké,Cláudia; Barone,Antonio Alci.
Herein, we present a synthesis of two publications that evaluate an abbreviated therapeutic approach to treating chronic hepatitis C virus (HCV) infection. Based on those publications, we discuss the use of the early virologic response (EVR) as a tool for the optimized management of patients under treatment, as well as reviewing concepts of HCV viral kinetics. The fourth-week EVR, characterized by HCV RNA dropping to undetectable levels, allows individuals infected with HCV genotype 1 and presenting low baseline viral loads to be treated with the combination of pegylated interferon and ribavirin for 24 weeks, whereas individuals infected with HCV genotypes 2 or 3 can be treated for only 12 weeks. Therefore, by adopting abbreviated treatment regimens...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV; Interferon; Viral kinetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100026

Liver histological alterations in patients with chronic hepatitis C and normal ALT levels in the city of Salvador, Northeast-Brazil
Provedor de dados: 55
Autores: Santana,Nelma Pereira de; Freitas,Luiz A.R. de; Lyra,André Castro; Paraná,Raymundo; Santana,Genoile; Trepo,Christian; Lyra,Luiz G.C.
Patients with chronic hepatitis C can have variable clinical progression. Hepatic histological alterations appear to be milder in asymptomatic subjects who have persistently normal ALT levels. AIMS: To evaluate the severity of histological liver alterations in blood donors with normal and elevated ALT levels. METHODS: We evaluated volunteer blood donors from the main blood bank of the city of Salvador-Brazil. Those who were anti-HCV positive were invited to participate in the study. Serum ALT and AST levels were measured at two time points, two months apart. Donors were divided into two groups: group I, individuals with ALT &gt; 1.5 times the upper limit of normal in at least one time point and group II, individuals with normal or near normal ALT, at both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ALT levels; Liver alterations; HCV; Northeast-Brazil.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000200003

Prevalence of selected sexually and blood-borne infections in Injecting drug abuser inmates of bandar abbas and roodan correction facilities, Iran, 2002
Provedor de dados: 55
Autores: Davoodian,Parivash; Dadvand,Habib; Mahoori,Khatereh; Amoozandeh,Alireza; Salavati,Alborz.
Human Immunodeficiency Virus (HIV) and other blood borne viral infections like hepatitis B virus (HBV) and hepatitis C virus (HCV) are major health issues especially in young and growing population of developing countries. All around the globe correctional facilities are known as potential source of spreading such disease. During summer 2002, HIV, HCV antibodies, hepatitis B surface (HBs) antigen and rapid plasma reagin (RPR) test were checked in venous blood samples of 252 injecting drug abuser prisoners from correctional facilities in southern Iran. Overall HIV, HCV and HBV infection rate was 15.1% (38/249), 64.8% (163/249), and 4.7% (12/249), respectively. HCV infection rate of HIV positive cases was 94% (35/38). All RPR results were negative. Duration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Iran; Prisons; HBV; HCV.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500008

Prevalence and risk factors for HBV, HCV and HDV infections among injecting drug users from Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Oliveira,M.L.A.; Bastos,F.I.; Telles,P.R.; Yoshida,C.F.T.; Schatzmayr,H.G.; Paetzold,U.; Pauli,G.; Schreier,E.
Viral hepatitis constitutes a major health issue, with high prevalence among injecting drug users (IDUs). The present study assessed the prevalence and risk determinants for hepatitis B, C and D viruses (HBV, HCV and HDV) infections among 102 IDUs from Rio de Janeiro, Brazil. Serological markers and HCV-RNA were detected by enzyme immunoassay and nested PCR, respectively. HCV genotyping was determined by restriction fragment length polymorphism analysis (RFLP). HBsAg, anti-HBc and anti-HBs were found in 7.8, 55.8 and 24.7% of IDUs, respectively. In the final logistic regression, HBV infection was independently associated with male homosexual intercourse within the last 5 years (odds ratio (OR) 3.1; 95% confidence interval (CI) 1.1-8.8). No subject...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HDV; HCV; HCV genotypes; IDU.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900009

Distribution of HCV genotypes among different exposure categories in Brazil
Provedor de dados: 56
Autores: Oliveira,M.L.A.; Bastos,F.I.; Sabino,R.R.; Paetzold,U.; Schreier,E.; Pauli,G.; Yoshida,C.F.T.
Hepatitis C virus (HCV) infection is widespread and responsible for more than 60% of chronic hepatitis cases. HCV presents a genetic variability which has led to viral classification into at least 6 genotypes and a series of subtypes. These variants present characteristic geographical distribution, but their association with different responses to treatment with interferon and severity of disease still remains controversial. The aim of this study was to investigate the patterns of distribution of HCV genotypes among different exposure categories in Brazil. Two hundred and fifty anti-HCV positive samples were submitted to HCV-RNA detection by RT-PCR and their genotype was determined by restriction fragment length polymorphism (RFLP) analysis. In addition,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: HCV; HCV genotypes; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300005

The vulnerability of Brazilian female prisoners to HIV infection
Provedor de dados: 56
Autores: Strazza,L.; Azevedo,R.S.; Carvalho,H.B.; Massad,E.
The purpose of the present study was to determine the vulnerability of women in prison to HIV infection. The study was carried out from August to October 2000 in a São Paulo State Penitentiary, where 299 female prisoners were serving time. We interviewed and obtained a blood sample from 290 females who agreed to enter the study. Sera were tested for the presence of antibodies to HIV, hepatitis C virus (HCV) and syphilis and the odds ratio (OR) was calculated for variables related to HIV positivity on the basis of a questionnaire. The overall prevalence data were: 13.9% for HIV (37 of 267), 22.8% for syphilis (66 of 290), and 16.2% for HCV (47 of 290). Sexual partnership variables were significantly related to HIV infection. These included HIV-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; HCV; Syphilis; Vulnerable populations; Women; Prisoners.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500020

Association of genotypes with viral load and biochemical markers in HCV-infected Sindhi patients
Provedor de dados: 58
Autores: Riaz,Saba; Bashir,Muhammad Faisal; Haider,Saleem; Rahid,Naeem.
Abstract The presented study had two objectives. The first was to examine distributions of Hepatitis C Virus (HCV) genotypes in Sindh, Pakistan, where HCV is prevalent. The other was to explore clinically relevant relationships between the genotypes, viral load (measured by real-time polymerase chain reaction assays) and biochemical markers. For this, 1471 HCV-infected patients in six cities in Sindh were recruited and sampled. HCV genotype distributions varied among the cities, but genotype 3a was most prevalent, followed by 3b, 1a and 1b (detected in 51.5, 22.7. 9.25 and 3.2% of the cases, respectively). No type-specific sequences were detected in serum samples from 189 (12.8%) of the 1471 patients. Frequencies of low (&lt;200,000 IU/mL serum),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sindh; HCV; Pakistan; Genotype; Biomarkers; Viral load.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400980

Diagnostic performance of an immunoassay for simultaneous detection of HCV core antigen and antibodies among haemodialysis patients
Provedor de dados: 58
Autores: El-Emshaty,Wafaa M; Raafat,Douaa; Elghannam,Doaa M; Saudy,Niveen; Eltoraby,Ehab E; Metwalli,Abd Elhameed A.
Nosocomial transmission of HCV is a concern in haemodialysis (HD) units worldwide. Diagnosis of HCV infection among dialysis patients is currently based on the detection of anti HCV antibodies by ELISA, and is confirmed by HCV RNA. The average window period between HCV infection and seroconversion with new generations of HCV antibody tests remains approximately 70 days with more prolonged period among dialysis patients. In this study we assessed the diagnostic performance of an immunoassay designed for simultaneous detection of anti HCV antibodies and core antigen in one step in comparison to qualitative RT-PCR and anti HCV antibodies detection test among Egyptian haemodialysis patients. The studied patients were 39 chronic renal failure patients on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Egypt; Haemodialysis; HCV; HCV core antigen; Immunoassay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100039

Seroprevalence of HBV and HCV markers among young adult males in the Air Force in Florianópolis, South Brazil
Provedor de dados: 60
Autores: Passos,Ana Maria; Strazer Neto,Miguel; Treitinger,Aricio; Spada,Celso.
We investigated the prevalence of hepatitis B virus (HBV) and hepatitis C virus (HCV) serological markers of infection in young adults from the metropolitan region of Florianópolis who were conscripts of the Air Base of Florianópolis in the state of Santa Catarina, Brazil. A population-based cross-sectional seroprevalence study was conducted with 371 young males during a one year period starting in June 2009. Demographic characteristics, socio-economic characteristics and possible risk factors to HBV and HCV were assessed. Blood samples were analyzed for HBsAg, anti-HBc, anti-HCV and anti-HBs through automated microparticle enzymatic immunoassays (Abbott®, AxSYM System, Wiesbaden, Germany). None of the participants showed positivity to HBsAg or anti-HCV....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HBV; HCV; Hepatitis B; Hepatitis C; Seroprevalence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200009