Consulta: subjectFacets:"HPLC"
Registros recuperados: 89
Data/hora: 05/06/2024 12:34:17

Propuesta y validación de una técnica de extracción de IMAZAPYR aplicado al suelo para su determinación por HPLC y espectroscopía NIR.
Provedor de dados: 32
Autores: Soto Barajas, Miltón Carlos.
Se propone un método que utiliza agua desionizada para la extracción de Imazapyr® del suelo.  La identificación y cuantificación del herbicida se realizó por cromatografía de líquidos de alta precisión (HPLC).  La validación del procedimiento de extracción se hizo considerando el desempeño de los siguientes parámetros: selectividad, recuperación, repetibilidad y robustez, de acuerdo a las definiciones del Centro Nacional de Metrología de México.  La recuperación del herbicida fue de 98% en promedio con el método propuesto, lo cual permite que se le considere como procedimiento de rutina en las investigaciones sobre disipación de este plaguicida. Alternativamente a la cuantificación del herbicida por cromatografía de líquidos de alta precisión (HPLC), se...
Palavras-chave: Extracción de Imazapyr; Validación de métodos; Detección de Imazapyr; HPLC; NIRS; Imazapyr TM extraction; Validation of methods; Imazapyr TM detection; Maestría; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/298

Actividad antimicrobiana y caracterización fenólica de extractos de cálices de jamaica (Hibiscus sabdariffa L.).
Provedor de dados: 32
Autores: Morales Cabrera, Mercedes.
En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de...
Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/568

Actividad antimicrobiana y caracterización fenólica de extractos de cálices de jamaica (Hibiscus sabdariffa L.).
Provedor de dados: 32
Autores: Morales Cabrera, Mercedes.
En este estudio se evaluó la actividad antimicrobiana de los extractos etanólicos, metanólicos y acuosos (frío y caliente) de cálices deshidratados de Hibiscus sabdariffa L. de cinco genotipos, sobre S. typhimurium (ATCC14028) y S. cholleraesuis (ATCC10708), se empleó el método difusión en agar. Se determinó la concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) por dilución. Se analizó la influencia del pH en la actividad antimicrobiana. Así mismo se realizó la caracterización fenólica de los extractos etanólicos. El efecto antimicrobiano fue variable; los extractos de la variedad Alma blanca (cálices verdes), presentaron el mayor efecto antimicrobiano (diámetros de inhibición de12.5 a 19.5 mm) sobre ambos serotipos de...
Palavras-chave: CMI; CMB; PH; Salmonella; Compuestos fenólicos; HPLC; MIC; MCB; Phenolic compounds; Fitopatología; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/568

Aerobic biodegradation of nonylphenol ethoxylates in shaking-flask test
Provedor de dados: 69
Autores: Wang,Ping; Nong,Xu-Hua; Ge,Jie-Hong.
Nonylphenol ethoxylates (NPEOs), which are widely used for industrial and domestic purposes, exert adverse effects on wildlife after being used and discharged into the environment. However, their ultimate biodegradability and biodegradation pathway remains unclear. In this study, the aerobic degradability of nonylphenol ethoxylates (NPEOs) by the acclimated microorganisms in active sludge was examined using shaking-flask tests. The degradation of benzene rings in NPEOs was determined using UV spectroscopy and high performance liquid chromatography (HPLC). Results showed that more than 80% of benzene rings were removed after 8-10 days of degradation, and the majority of NPEOs were also removed after 9 days of degradation, indicating NPEOs and the benzene...
Tipo: Journal article
Palavras-chave: Aerobic biodegradation; Degradation pathway; ESI-MS; HPLC; Nonylphenol ethoxylates.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400001

Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter diazotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC).
Provedor de dados: 14
Autores: RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAUJO, J. L. S. de; BALDANI, J. I.
A espécie Gluconacetobacter diazotrophicus é uma bactéria endofítica reconhecida pela sua capacidade de fixação biológica de nitrogênio (FBN) em associação com a cultura de cana-de-açúcar. Além da FBN, a produção de auxinas tem sido considerada como um fator adicional na promoção do crescimento desta cultura. Neste estudo, foi descrita uma metodologia para a identificação e quantificação de compostos indólicos presentes no sobrenadante de Gluconacetobacter diazotrophicus, crescida em meio líquido, utilizando a técnica de cromatografia líquida de alta eficiência (HPLC) e extração em fase sólida (SPE). Com esta metodologia foi possível realizar a detecção por HPLC de ácido indol pirúvico no sobrenadante de G. diazotrophicus sugerindo que a rota do IPyA foi...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Compostos indólicos; HPLC.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/664190

Monthly HPLC measurements of pigment concentration from an intertidal muddy sediment of Marennes-Oléron Bay, France
Provedor de dados: 5
Autores: Cariou-le Gall, Valerie; Blanchard, Gerard.
Chloropigments and carotenoids were measured by HPLC in an intertidal muddy sediment of Marennes-Oleron Bay (France). Concentrations were determined as a function of sediment depth at low tide. The analyses were carried out at monthly intervals over 1 yr. Pigment analysis indicated that the microphytobenthic community was dominated by diatoms throughout the sampling year. Chlorophyll b was not encountered at any time or depth, indicating that no input from macrophytic detritus had occurred at the sampling site. There was a large pool of phaeopigments a, of which phaeophorbides were the major forms (75%). A microphytobenthic bloom occurred between March and June during which phaeopigments significantly increased with a high proportion of phaeophorbides...
Tipo: Text
Palavras-chave: Pigment; HPLC; Intertidal mudflat; Microphytobenthos; Seasonal change.
Ano: 1995
URL: http://archimer.ifremer.fr/doc/00257/36844/36666.pdf

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities
Provedor de dados: 5
Autores: Stachowski-haberkorn, Sabine; Quiniou, Louis; Beker, Beatriz; Haberkorn, Hansy; Marie, Dominique; De La Broise, Denis.
The impacts of the fungicide Opus(A (R)) (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 mu g l(-1) of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 mu g l(-1), TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant...
Tipo: Text
Palavras-chave: Microcosm; Phytoplankton community; Epoxiconazole; TTGE; Flow cytometry; HPLC.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00088/19888/17547.pdf

Monosaccharide composition of suspended organic particulate matter in relation to its origin
Provedor de dados: 5
Autores: Compiano, Am; Romano, Jc; Delagiraudiere, I; Laborde, P.
Samples of phytoplankton cultures and of suspended particulate matter from continental, brackish and marine waters were examined by HPLC for the monosaccharide content of their hydrolysable sugar fraction. Eight monosaccharides were currently assayed and their weight compared to HPLC-assayed chlorophyll a concentrations. With the exception of oligotrophic waters, these two variables are well correlated in the various prospected environments. Glucose was always the strongly dominant form, not only in fresh and brackish waters but also in marine samples with high phytoplanktonic biomass. If samples are compared on a salinity basis, only four monosaccharides showed significant differences in their relative concentrations, as a consequence of sampling...
Tipo: Text
Palavras-chave: MONOSACCHARIDE; HPLC; PARTICULATE MATTER; SEA WATER; BRACKISH WATER.
Ano: 1993
URL: http://archimer.ifremer.fr/doc/00100/21134/18754.pdf

Evaluation of FluoroProbe® performance for the phytoplankton-based assessment of the ecological status of Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Garrido, Marie; Cecchi, Philippe; Malet, Nathalie; Bec, Beatrice; Torre, Franck; Pasqualini, Vanina.
The European Water Framework Directive and several other legislations worldwide have selected phytoplankton for monitoring the ecological status of surface waters. This assessment is a complicated task in coastal lagoons due to their intrinsic variability, prompting moves to use real-time measurements. Here, we tested the ability of the submersible spectrofluorometer FluoroProbe® to accurately estimate the phytoplankton biomass and to efficiently discriminate spectral groups in Mediterranean coastal lagoons, by using sub-surface water samples (n = 107) collected at Biguglia lagoon (Corsica) in different environmental situations (salinity and trophic state) from March 2012 to December 2014. We compared the estimates of biomass and phytoplankton group...
Tipo: Text
Palavras-chave: Phytoplankton; Monitoring; Mediterranean coastal lagoons; FluoroProbe (R); HPLC.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00484/59582/62623.pdf

Sources et caractéristiques des composés phénoliques dans l’estuaire de la Seine
Provedor de dados: 5
Autores: Motamed, B; Texier, H.
Within the framework of the multi-disciplinary program Seine Aval, phenolic compounds obtained by CuO oxidation have been studied since 1995. Quantitative analyses have shown a terrestrial contribution in flood, characterized by compounds of the group P (polyols + phenol), S (syringic) and H (hydroxybenzoic). During the low water period, phenolic compounds allow a clear distinction between fluvial and estuarine suspended material. Fluvial run-off contained Syringic, Hydroxybenzoic and Vanillic phenols. In contrast, upstream penetrating marine and estuarine waters are characterized by cinnamic phenols originating from estuarine herbus. In the turbidity maximum zone, aerobic degradation of lignin material increases the (vanillic acid/vanillin) ratio.
Tipo: Text
Palavras-chave: Composés phénoliques; HPLC; Matière en suspension; Seine; Phenolic compounds; HPLC; Suspended matter; Seine.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/00325/43570/44051.pdf

CHEMOTAXONOMIE PIGMENTAIRE - Initiation aux calculs appliqués au phytoplancton
Provedor de dados: 5
Autores: Lampert, Luis.
Ce travail recueille l’ensemble de procédures pour une approche chémotaxonomique des analyses pigmentaires par HPLC. Il décrit plus particulièrement les méthodes « Diagnostic Pigments » (DP), les régressions linéaires multiples (RLM) et l’utilisation du logiciel ©CHEMTAX 1.95 (CSIRO, Australie)
Tipo: Text
Palavras-chave: CHEMTAX; Chémotaxonomie; HPLC; Pigments phytoplanctoniques.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00226/33714/32130.pdf

Application de l’indice de composition pigmentaire ICBC pour Manche-Atlantique aux masses d’eau méditerranéennes
Provedor de dados: 5
Autores: Lampert, Luis; Hernandez Farinas, Tania.
Suite aux  études concernant la mise en place d’un indice de composition pigmentaire dans les eaux de Manche-Atlantique (Lampert, 2018) et en Méditerranée (Goffart, 2018), il a été convenu de procéder à un échange de données pour ces deux façades afin d’évaluer le potentiel de ces deux indicateurs sur les façades pour lesquelles ils n’ont pas été conçus. Nous avons donc, appliqué l’indice de composition ICBC aux données obtenues entre 2009 et 2017 en Méditerranée (ICBCmed), défini une grille de classement pour l’EQR IC et finalement classé les masses d’eau afin d’inclure l’indice de composition dans l’indicateur phytoplancton de Méditerranée. Cet ICBCmed a été calculé pour les 15 points échantillonnés. Le résultat du classement est cohérent avec les...
Tipo: Text
Palavras-chave: MEDITERRANEE; DCE; HPLC; PHYTOPLANCTON; INDICE DE COMPOSITION; ICBC.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00469/58111/60531.pdf

Pigment dynamics associated with the grazing of a ciliate and a flagellate feeding on a cyanobacterium
Provedor de dados: 5
Autores: Mostajir, B; Bustillos-guzman, J; Claustre, H; Rassoulzadegan, F.
The egestion of particulate material as well as pigment degradation during microzooplankton grazing on phytoplankton are poorly known processes. In an attempt to evaluate these processes, changes in pigment concentrations within various size fractions were monitored in batch cultures of an assemblage of a pelagic ciliate (Strombidium sulcatum) and a heterotrophic flagellate (Paraphysomonas sp.) feeding on a cyanobacterium (Synechococcus sp.) over a 10-day period. Chlorophyll a, carotenoids and phaeopigments were not found in the 0.1-0.7 mu m fraction while the pigments originally in the 0.7-3.0 mu m fraction (prey) were transferred into the &gt; 3.0 mu m size fraction (predator). During this transfer, the carotenoids (zeaxanthin and B-carotene) were not...
Tipo: Text
Palavras-chave: Pigment; Phéopigment; Broutage; Protozoaire; HPLC; Pigment; Phaeopigment; Grazing; Protozoa; HPLC.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/00325/43660/43209.pdf

Phytoplankton strategies to exploit nutrients in coastal lagoons with different eutrophication status during re-oligotrophication
Provedor de dados: 5
Autores: Leruste, A; Guilhaumon, F; De Wit, R; Malet, Nathalie; Collos, Y; Bec, Beatrice.
We studied a mesotrophic and a hypertrophic Mediterranean coastal lagoon, both of which had been simultaneously subjected to a nutrient input reduction for 9 yr. We compared these 2 lagoons to an oligotrophic coastal lagoon. Using bioassays comprising 24 h incubations with added phosphorus and/or ammonium, we investigated the response of the phytoplankton communities to nutrient enrichment during summer in terms of biomass, size class structure, abundance and growth. For nitrogen and phosphorus, we identified which nutrient limited phytoplankton growth, and what strategies of nutrient exploitation the communities adopted to cope with these limitations. Ultraphytoplankton dominated the 3 communities, but it differed in composition among the lagoons. Green...
Tipo: Text
Palavras-chave: Growth rate; Nutrient limitation; Dilution experiment; HPLC.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00508/61978/66084.pdf

Identification et quantification des pigments chlorophylliens et caroténoïdes des sédiments marins : un protocole d'analyse par HPLC
Provedor de dados: 5
Autores: Brotas, V; Plantecuny, Mr.
A HPLC protocol is described which permits analysis of the pigment composition of coastal and intertidal sediments. The HPLC technique has been extensively applied to phytoplankton pigments for fifteen years. Papers dealing with living benthic microphytic populations have been more rare, due to complexity existing in the sediment surface, where pigments (chlorophylls and carotenoids) from several taxonomic groups of microalgae are mixed with degraded pigments from senescent phytoplankton cells and macrophytic material. The present paper describes in detail a protocol adapted from Kraay et al. (1992), which allows a good discrimination of photosynthetic pigments of microphytobenthos communities. The following procedure is recommended. Sediment samples are...
Tipo: Text
Palavras-chave: Plants pigments; Sediments; Protocol; HPLC.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00096/20730/18367.pdf

Photo-protective compounds in red macroalgae from Brittany: Considerable diversity in mycosporine-like amino acids (MAAs)
Provedor de dados: 5
Autores: Lalegerie, Fanny; Lajili, Sirine; Bedoux, Gilles; Taupin, Laure; Stiger-pouvreau, Valerie; Connan, Solène.
To cope with the biotic and abiotic stresses experienced within their environment, marine macroalgae have developed certain defence mechanisms including the synthesis of photo-protective molecules against light and particularly harmful UV radiation. The aim of this study was to screen selected red algae, a highly diverse phylogenetic group, for the production of photo-protective molecules. The pigment content and composition (i.e. chlorophyll-a, phycobiliproteins and carotenoids) and the composition of mycosporine-like amino acids (MAAs) were studied in 40 species of red macroalgae collected in Brittany (France), at two distinct periods (i.e. February and July 2017). A high inter-specific variability was demonstrated in terms of pigment content and MAA...
Tipo: Text
Palavras-chave: Algae; Diversity; HPLC; MAAs; Metabolites; Photo-protection; Pigments; Rhodophyta; Screening.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00488/59929/63133.pdf

Test d’un Indice de composition pigmentaire pour les secteurs Atlantique et Manche (DCE)
Provedor de dados: 5
Autores: Lampert, Luis.
Afin de produire des Indices de Composition pigmentaires (Ic), les indices de similarité de Bray-Curtis et de Kulczynski ont été calculés sur les matrices pigmentaires brutes (HPLC) et confrontés aux indicateurs de pressions disponibles, essentiellement nutriments et biomasse chlorophyllienne. Un volet est dédié à la façade atlantique et un autre à la Manche orientale.
Tipo: Text
Palavras-chave: DCE; Indice de composition; Chémotaxonomie; HPLC; Pigments phytoplanctoniques; Manche; Atlantique.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00254/36556/35101.pdf

Les détergents non-ioniques dans les stations d'épuration
Provedor de dados: 5
Autores: Porot, V.
Nonionic detergents of nonylphenolpolyethoxylate type, have been analyzed and identified for each dligomer by high performance liquid .' chromatography, in primary and secondary effluents of the sewage treatment plants Toulon and Morlaix. Measured concentrati~ns for each oligomer indicate variations on 24 hours CyClE and great differences:between sewage treatment.
Tipo: Text
Palavras-chave: Détergents non-ioniques; Nonylphénolpolyéthoxylates; HPLC; : station d'épuration.; Nonionic detergents; Nonylphénolpolyethoxylates; HPLC; Sewage ...t.r..e..a..tm.en.t.. .:cJ. an t.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00080/19134/16729.pdf

Etude chémotaxonomique de la campagne TIC-MOC (mars 2015) – Projet CALHIS
Provedor de dados: 5
Autores: Lampert, Luis; Perrot, Laurie; Delmas, Daniel.
A partir des échantillons collectés lors de la campagne TIC-MOC (mars 2015) sur le plateau continental argentin, des analyses en pigments phytoplanctoniques permettent de quantifier la distribution des principales classes algales par la méthode CHEMTAX. Les Prochlorophycées et cyanobactéries sont essentiellement dominantes dans les eaux chaudes du courant du Brésil, tandis que dans les eaux plus froides du courant des Malouines et la zone de mélange (T°&lt; 20°C), c’est les Haptophytes (Phaeocystis et Emiliania huxleyi) qui dominent la biomasse chlorophyllienne. Des dominances moyennes en dinoflagellés supérieures 30% sont observées dans les deux masses d’eau.
Tipo: Text
Palavras-chave: Chémotaxonomie; Coccolithophoridés; HPLC; Pigments phytoplanctoniques; Patagonie; Plateau continental.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00327/43784/43342.pdf

Caractérisation chimique des composés humiques et de leurs diverses classes de poids moléculaires dans les dépôts du delta du Rhône
Provedor de dados: 5
Autores: Gadel, F; Charriere, B; Serve, L; Comellas, L.
The analysis of humic compounds by elemental analysis, PY-GC-MS and HPLC techniques permitted determination of the origin of organic inputs, essentially terrestrial, and the effects of environmental conditions in the deposits along a north-south transect through the Rhone delta, from the river mouth to the open sea. In the prodelta the sedimentation of fine particles enriches the sediments in organic matter. The humic compound contents were relatively low in sediments, perhaps due to a rapid burying of organic matter which leads to anoxic conditions. In sediments fulvic acids are more abundant than humic acids. Elemental analysis indicated that fulvic acids were enriched in hydrogen and nitrogen as compared to humic acids. Furthermore, H/C and N/C ratios...
Tipo: Text
Palavras-chave: HUMIC COMPOUNDS; MOLECULAR WEIGHT CLASSES; PY-GC-MS; HPLC; RHONE DELTA.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00101/21187/18804.pdf

Détermination d’un indice de composition phytoplanctonique pigmentaire pour les eaux de la Manche et de l’Atlantique (DCE)
Provedor de dados: 5
Autores: Lampert, Luis; Hernandez Farinas, Tania.
La demande de la part de la Commission Européenne, pour inclure un Indice de Compositions (IC) dans l’indicateur « phytoplancton » de la Directive Cadre de l’Eau, a été prise en compte et un test en conditions réelle a été mené sur les données pigmentaires acquises entre 2016 et 2017. Deux échantillons par mois ont été analysés par chromatographie liquide (HPLC) sur 16 points, situés de la frontière belge et jusqu’au nord de la Loire. Cet indice (ICBC) a été bâti par la comparaison des moyennes pigmentaires de chaque point à une référence, via l’indice de similarité de Bray-Curtis. Cette référence (REF-Atl) a été définie comme la moyenne des deux points les moins impactées (Querqueville et Les Hébihens). La fréquence d’échantillonnage retenue est celle de...
Tipo: Text
Palavras-chave: MANCHE; ATLANTIQUE; DCE; HPLC; PHYTOPLANCTON; INDICE DE COMPOSITION; ICBC.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00469/58110/60530.pdf

Summary Proceedings of the Second Coordinators' Meeting on Peanut Stripe Virus
Provedor de dados: 30
Palavras-chave: Viruses; Groundnuts; Genotypes; Yield losses; ELISA; Peptides; Quarantine; Legumes; Germplasm; HPLC.
Ano: 1989
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4070

Ten Myths about Tannins in Sorghums
Provedor de dados: 30
Autores: Rooney, L.
Palavras-chave: Organic acids; Sorghum; Antioxidants; Pigments; Phenols; Linseed; Aromatic compounds; HPLC; Bakery products; Polymers.
Ano: 2005
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3264

Effects of genetics and environment on isoflavone content of soybean from different regions of Brazil
Provedor de dados: 86
Autores: Carrão-Panizzi,Mercedes Concórdia; Beléia,Adelaide Del Pino; Kitamura,Keisuke; Oliveira,Maria Cristina Neves.
The effects of genetics and environmental factors on isoflavone content of soybean (Glycine max L.) cultivars grown in different locations in Brazil in 1993/94 were evaluated. Seeds of different cultivars were analised by high performance liquid chromatography (HPLC). In Rio Grande do Sul (RS), Paraná (PR), and Mato Grosso do Sul (MS) States, a significant difference in the isoflavone total content average of the cultivars IAS 5 and FT-Abyara (163.9, 116.4 and 79.5 mg/100 g, respectively) was observed. In general, IAS 5 contained higher isoflavone than FT-Abyara. Cultivars IAS 5 and FT-Abyara grown at Vacaria, RS (28°30' S latitude) with temperature average of 19°C, had the highest isoflavone concentrations (218.7 and 163.8 mg/100 g, respectively). In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Daidzin; Genistin; Crop location; HPLC.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000004

Sorption and desorption of picloram in soils under pastures in Brazil
Provedor de dados: 87
Autores: Assis,E.C; Silva,A.A; Barbosa,L.C; Queiroz,M.E.L.R; D'Antonino,L; Cruz,L.S.
The objective of this work was to determine the coefficients of sorption and desorption of picloram in Ultisol (PVA) and Oxisol (LVA), displaying different physical and chemical characteristics. Samples of soil were collected at the 0 20 cm depth in degraded pasture areas in Viçosa-MG. Firstly, the equilibrium time between the herbicide in solution and the herbicide which was sorbed in the soil was determined by the Batch Equilibrium method. The time required was 24 hours. Sorption and desorption studies were carried out under controlled laboratory conditions; the sorption evaluation consisted in adding 10.0 mL of herbicide solutions at different concentrations to tubes containing 2.00 g of soil, with vertical rotary agitation being maintained during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbicide; Batch equilibrium; Freundlich isotherms; HPLC.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000400020

Lixiviação do glyphosate e do imazapyr em solos com diferentes texturas e composição química: II. método analítico
Provedor de dados: 87
Autores: Souza,Adailson P. de; Prates,Hélio T.; Ferreira,Francisco A.; Reis,Efraim L.; Jordão,Claudio P.
Em razão das poucas de informações existente na literatura, sobre a mobilidade do glyphosate e do imazapyr, em solos de baixa atividade, comum em países tropicais, foi conduzido, em laboratório, um estudo sobre a mobilidade vertical desses herbicida s em dois solos com diferente s composições químicas e fisicas. Para estudar essa mobilidade, foram utilizadas colunas de solo com diferentes alturas, as quais, após aplicação dos produtos na dose comerc ial de 4 L ha-1, foram submetida s a um regime hídrico de 40 mm h-1, por um período de 4 h. As análises dos resíduos nas diferentes profundidades foram feitas utilizando-se os métodos voltamétrico (polarografia) e HPLC, para o glyphosate e imazapyr, respectivame nte. Com base nos resultados, foi possível...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbicidas; Voltametria; Polarografia; Cromatografia; HPLC.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83581999000200009

Microwave-assisted solvent extraction and analysis of shikimic acid from plant tissues
Provedor de dados: 87
Autores: Matallo,M.B; Almeida,S.D.B; Cerdeira,A.L; Franco,D.A; Blanco,F.M.G; Menezes,P.T.C; Luchini,L.C; Moura,M.A.M; Duke,S.O.
A better method for determination of shikimate in plant tissues is needed to monitor exposure of plants to the herbicide glyphosate [N-(phosphonomethyl)glycine] and to screen the plant kingdom for high levels of this valuable phytochemical precursor to the pharmaceutical oseltamivir. A simple, rapid, and efficient method using microwave-assisted extraction (MWAE) with water as the extraction solvent was developed for the determination of shikimic acid in plant tissues. High performance liquid chromatography was used for the separation of shikimic acid, and chromatographic data were acquired using photodiode array detection. This MWAE technique was successful in recovering shikimic acid from a series of fortified plant tissues at more than 90% efficiency...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glyphosate; Green chemistry; Herbicide; HPLC.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000500012

VINASSE EFFECT ON HERBICIDES CLOMAZONE AND TEBUTHIURON AVAILABILITY IN DIFFERENT KINDS OF SOILS
Provedor de dados: 87
Autores: MATOS,A.K.A.; CARBONARI,C.A.; VELINI,E.D.; GOMES,G.L.G.C.; TRINDADE,M.L.B.; MACEDO,G.C.
ABSTRACT The objective of this study was to evaluate the availability of herbicides clomazone and tebuthiuron in the solution in different kinds of soils saturated with water or vinasse. Samples of 30 soils with different characteristics were arranged on trays to the herbicides spraying. Then they were homogenized, placed in plastic cartridges and saturated with deionized water or vinasse, and remaining at rest during 18 hours. Two extractions were made, the first one quantified the presence of the herbicides in the soil solution and in the second one the total extraction of herbicide remaining in the soil was taken to determine the recovery percentage of each herbicide tested. For quantification, a LC-MS/MS system was used, a compound of a high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Mass spectrometry; HPLC; Sorption.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000400771

Glyphosate as a tool to produce shikimic acid in plants
Provedor de dados: 87
Autores: Matallo,M.B.; Almeida,S.D.B.; Franco,D.A.S.; Cerdeira,A.L.; Gazzeiro,D.L.P.
Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha¹) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbicides; Corn; Soybean; Oseltamivir; Pandemics; HPLC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000300016

Distribuição de massa molecular de ácidos húmicos e promoção do crescimento radicular
Provedor de dados: 90
Autores: Aguiar,Natália de Oliveira; Canellas,Luciano Pasqualoto; Dobbss,Leonardo Barros; Zandonadi,Daniel Basílio; Olivares,Fábio Lopes; Façanha,Arnoldo Rocha.
Os ácidos húmicos (AH) estimulam diretamente vários processos fisiológicos que promovem o crescimento vegetal, especialmente do sistema radicular. O conhecimento da natureza química e do papel de AH na expressão de efeitos biofertilizantes e bioestimulantes é fundamental para o desenvolvimento de insumos biológicos à base de ácidos húmicos. O objetivo deste trabalho foi avaliar uma possível relação entre a distribuição de massa molecular aparente de AH isolados de vermicomposto e a magnitude de resposta na promoção do crescimento radicular. Para isso, foram obtidas subfrações (SF) dos AH por meio de cromatografia preparativa por exclusão de tamanho em gel de Sephadex G-50 (CGE). O processo preparativo foi validado pelo uso da cromatografia líquida de alta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Efeitos fisiológicos; Estrutura e conformação; Cromatografia em gel; HPLC.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000600010

Contaminação de águas superficiais por agrotóxicos em função do uso do solo numa microbacia hidrográfica de Agudo, RS
Provedor de dados: 91
Autores: Bortoluzzi,Edson C.; Rheinheimer,Danilo dos S.; Gonçalves,Celso S.; Pellegrini,João B.R.; Zanella,Renato; Copetti,André C.C.
Realizou-se este trabalho com o intuito de quantificar a presença de moléculas de agrotóxicos em águas superficiais de três unidades paisagísticas (UP) com diferentes configurações de uso, numa microbacia hidrográfica de cabeceira em Agudo, RS. Dois coletores semi-automáticos de água foram instalados no córrego de cada UP, onde se coletaram amostras, por ocasião de chuvas, em três épocas da cultura do fumo: transplante, aterramento junto aos pés da cultura (planta com 6-10 folhas) e colheita. Os princípios ativos imidacloprid, atrazina, simazina e clomazone foram quantificados por Cromatografia Líquida de Alta Eficiência com Detecção no Ultravioleta (HPLC-UV). Clorpirifós, flumetralin e iprodione foram analisados por Cromatografia Gasosa com Detecção por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SIG; Erosão; HPLC; Paisagem; Fumo.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662006000400015

Xylose production from sugarcane bagasse by surface response methodology
Provedor de dados: 91
Autores: Paiva,José E. de; Maldonade,Iriani R.; Scamparini,Adilma R. P.
The aim of this study was to optimize the production of xylose from sugarcane bagasse. The hydrolysis process was carried out to evaluate the effect of temperature and sulphuric acid concentration on the xylose production at 18, 30, and 50 min of hydrolysis. A Central Composite Rotatable Design (CCRD) was used to study two process variables, temperature (111.5; 114.0; 120.0; 126.0 and 128.5 ºC) and sulphuric acid concentration (0.20; 0.70; 1.90; 3.10 and 3.60% v v-1). Sulphuric acid had a greater influence on the hydrolysis process than temperature. At concentrations of sulphuric acid higher than 3.10% (v v-1), raising temperature had little influence on the yield of xylose production. The best condition to obtain xylose (266.73 mg g-1 of dry bagasse) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemicellulose; Hydrolysis; HPLC; Aldopentose; Renewable carbon source.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662009000100011

Influence of photoperiod on the accumulation of allantoin in comfrey plants
Provedor de dados: 93
Autores: CASTRO,ANA HORTÊNCIA FONSÊCA; YOUNG,MARIA CLÁUDIA MARX; ALVARENGA,AMAURI ALVES DE; ALVES,JOSÉ DONIZETI.
In order to evaluate the influence of photoperiod on the accumulation of allantoin in comfrey (Symphytum officinale L.), a well known medicinal plant, an experiment was conducted during July and August, 1998. Cuttings obtained from 90 day old plants were submitted to four photoperiods (8, 12, 16 and 20 hours). After 60 days, allantoin content in roots and rhizomes was evaluated. The results showed that increases in photoperiod promoted an increment in the average content of allantoin in roots (0.06%, 0.303%, 1.213% and 4.78%). On the other hand, in rhizomes, allantoin accumulation decreased (9.65%, 7.14%, 0.55%) when the photoperiod was increased from 8 to 12 and 16 hours, respectively, stabilizing on a 20-hour photoperiod (0.53%). Plants cultivated under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Medicinal plant; Secondary metabolism; Symphytum officinale; Ureides.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100006

QUANTIFICAÇÃO DE AÇÚCARES EM PÊSSEGOS DA VARIEDADE BIUTI, ARMAZENADOS SOB CONDIÇÕES DE AMBIENTE E REFRIGERAÇÃO
Provedor de dados: 94
Autores: OLIVEIRA,MARCELO ALVARES DE; CEREDA,MANRNEY PASCOLI; CABELLO,CLÁUDIO; URBANO,LUIZ HENRIQUE.
O objetivo deste trabalho foi identificar e quantificar os açúcares em frutos de pessegueiro da variedade 'Biuti', armazenados sob condições de ambiente (27,3ºC; 70% UR) e sob refrigeração (4ºC; 90%), comparar as diferenças entre os teores de açúcares nas duas condições de armazenamento. A identificação e a quantificação precisa dos açúcares (glicose, frutose e sacarose) foi realizada por cromatografia em fase líquida HPLC. Verificou-se que a sacarose foi o açúcar encontrado em maior quantidade, sendo verificado apenas traços de glicose e frutose em alguns frutos. Sob condições ambientais, os teores de sacarose do 3º até o 9º dia de armazenamento não diferiram significativamente entre si; entretanto, no 12º dia, os frutos obtiveram baixos teores de...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Armazenamento; HPLC; Sacarose; Frutose e glicose.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452001000200046

2,6-Diaminopimelic acid (DAPA) in microbial protein quantification of heifers fed different forage sources
Provedor de dados: 96
Autores: Couto Filho,Cristovão Colombo de Carvalho; Saliba,Eloísa de Oliveira Simões; Rodriguez,Norberto Mario; Barbosa,Geraldo Sérgio Senra Carneiro; Freitas,Regeane Martins de; Resende,Matheus Pinheiro Diniz.
The objective of this study was to evaluate the flow of nitrogenous compounds, protein degradability, rumen degradation of total carbohydrate and organic matter and microbial efficiency in heifers subjected to diets containing corn silage, sugarcane or Tifton. For this purpose, the 2,6-diaminopimelic acid (DAPA) technique was adopted and analytical procedures for amino acids by HPLC were adapted. Six rumen-fistulated Holstein-Zebu heifers with 480 kg of initial BW and at 24 months of age kept in individual tie stalls were assigned to two 3 × 3 Latin squares. Omasal digesta dry matter and microbial dry matter flows were determined using the isolated, purified and enriched lignin (LIPE®) and DAPA markers, respectively. Isolated bacteria from rumen showed on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn silage; Flow of nitrogen compounds; HPLC; Microbial efficiency; Protein degradability; Sugarcane.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015000500180

Effects of enzyme complex SSF (solid state fermentation) in pellet diets for Nile tilapia
Provedor de dados: 96
Autores: Moura,Guilherme de Souza; Lanna,Eduardo Arruda Teixeira; Filer,Keith; Falkoski,Daniel Luciano; Donzele,Juarez Lopes; Oliveira,Maria Goreti de Almeida; Rezende,Sebastião Tavares de.
The effects of enzyme complex SSF (solid state fermentation) on growth performance and the availability of sucrose and monosaccharides in the chyme of Nile were involved. The study included 360 fish (70g±4.43) in a completely randomized design with six dietary treatments (0, 50, 100, 150, 200 and 250 ppm of SSF) arranged in six replicates, with 10 fish per replicate. Every 15 days, one tilapia of each experimental unit was sacrificed for analyses of carbohydrate in the chyme. On day 60 of the experiment, the performance parameters were measured. There was a linear effect according to treatment for final weight and weight gain. For the other performance parameters, there were no differences. There was quadratic effect for sucrose and glucose in function of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquaculture; Enzymes; Fish nutrition; HPLC; Oreochromis niloticus.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000001

Evaluación fitoquímica de hojas de Excoecaria lucida Sw. (Aité) (Euphorbiaceae) y Aislamiento e Identificación de un hemiterpenoide
Provedor de dados: 98
Autores: Ochoa Pacheco,Ania; Escalona Arranz,Julio César; Fechine Tavares,Josean; Sobral Da Silva,Marcelo.
Tipo: Journal article
Palavras-chave: Excoecaria lucida; Hemiterpenoide; Resonancia Magnética Nuclear; HPLC.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000100011

Método para la cuantificación de cumarina en extracto seco a partir de extractos de Justicia pectoralis Jacq.
Provedor de dados: 98
Autores: Rodríguez Chanfrau,Jorge E.; López Hernández,Orestes D.; Gil Apan,José M.
Fundamentos: Justicia pectoralis Jacq., especie de la familia Acantaceae, es una planta herbácea que se emplea como sedante tradicionalmente por la población cubana. Objetivos: desarrollar y validar un método de análisis por cromatografía líquida de alta resolución para la determinación de cumarina en el extracto seco obtenido a partir de extractos de J. pectoralis. Métodos: se desarrolla y valida un método de análisis por cromatografía líquida de alta resolución para la determinación de cumarina. Resultados: el método desarrollado es específico, preciso, exacto y lineal. Conclusiones: el método puede ser empleado para cuantificar cumarina, en muestras de polvo seco obtenidas mediante secado por aspersión a partir de extractos de J. pectoralis.
Tipo: Journal article
Palavras-chave: Cumarina; Método de análisis; Justicia pectoralis; Validación de métodos de análisis; HPLC; Control de calidad.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962008000300004

Desarrollo y validación de un método de cromatografía líquida de alta resolución para la determinación y cuantificación de clenbuterol en hígado de bovino
Provedor de dados: 101
Autores: Morales-Trejo,F; Vega y León,S; Escobar-Medina,A; Pérez-González,J.J; Urbán-Carrillo,G; Gutiérrez-Tolentino,R.
Se desarrolló una metodología para la determinación de clenbuterol en hígado de bovino por cromatografía líquida de alta resolución (HPLC). La extracción se realizó con acetonitrilo e isopropanol. El extracto se analizó empleando una columna de fase reversa C18, un detector UV a 214 nm y una mezcla de NaH2PO4 0.05M (pH 3.0)/acetonitrilo (85:15, v/v) como fase móvil. El tiempo de retención para el clenbuterol fue 24.82 min. La recta de mejor ajuste de la curva de calibración del estándar fue y = 1340.8563x - 1539.7319, con un r²=0.9997 (p&lt;0.05). Los límites de detección y de cuantificación mostraron valores de 0.9 y 1.8 ng/mL, respectivamente. Las medias de recuperación de clenbuterol fueron de 111.7, 82.0 y 84.8%, para tres niveles de concentración...
Tipo: Journal article
Palavras-chave: Clenbuterol; HPLC; Validación; Hígado de bovino.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100006

Evaluación de aflatoxina M1 en leche orgánica producida en Tecpatán, Chiapas, México
Provedor de dados: 101
Autores: Gutiérrez,R; Vega,S; Pérez,J.J; Ruiz,J.L; Yamazaki,A; Rivera,J.G; Urbán,Georgina; Escobar,A.
Con la finalidad de demostrar la inocuidad de la leche orgánica producida en Tecpatán, Chiapas, México, se determinó la presencia de aflatoxina M1 por cromatografía líquido de alta resolución (HPLC). Los resultados mostraron que el 23.3 % de las muestras sobrepasó el límite máximo de residuo propuesto por la regulación mexicana de 0.5 µg kg-1, mientras el 62.7% sobrepasaba el valor de 0.05 µg kg-1 de la UE. Los valores medios de aflatoxina M1 encontrados en la leche durante las épocas de seca y lluvia fueron de 3.53± 0.55 µg kg-¹ y 0.17 ± 0.13 µg kg-1 respectivamente. Los resultados corroboran que los productos orgánicos pueden estar expuestos a la presencia de contaminantes cuando no se cumplen las buenas prácticas agrícolas, afectando su inocuidad.
Tipo: Journal article
Palavras-chave: Aflatoxina M1; Leche orgánica; México; HPLC.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100005

VALIDACIÓN DE UN MÉTODO DE CROMATOGRAFÍA LÍQUIDA DE ALTA RESOLUCIÓN (CLAR), EMPLEANDO COMO MARCADOR LA EPIGALOCATEQUINGALATO,PARA EVALUAR LA CALIDAD DE UNA FORMULACIÓN SEMISÓLIDA OBTENIDA DE Rhizophora mangle L.
Provedor de dados: 101
Autores: Escobar,A; Faure,R; Sosa,Dayana; Bueno,Tania; Rodríguez,Yanet; Soler,Dulce M.
Con el objetivo de establecer un marcador químico de polifenoles y validar un método por cromatografía líquida de alta resolución (CLAR) para evaluar la calidad de productos semisólidos obtenidos a partir del extracto acuoso de la corteza de Rhizophora mangle L, se compararon cuatro procedimientos de extracción del analito de la muestra; donde la variante que emplea etil acetato y su aplicación directa al cromatógrafo fue la más efectiva. De los diferentes polifenoles identificados en la muestra, la epigalocatequingalato (EGCG) fue seleccionada como marcador. El producto semisólido en condiciones de estrés oxidativo muestra una disminución del marcador y cambios en el perfil cromatográfico. Los parámetros de desempeño del método revelan una linealidad en...
Tipo: Journal article
Palavras-chave: Rhizophora mangle L.; Semisólido; EGCG; Polifenoles; HPLC; Validación.
Ano: 2011
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2011000300004

DETERMINACIÓN DE CLORTETRACICLINA EN PIENSO POR CROMATOGRAFÍA LÍQUIDA DE ALTA RESOLUCIÓN (CLAR)
Provedor de dados: 101
Autores: Escobar,A; Oliva,Yuleivys; Abeledo,María Antonia; Vega,E; Cedeño,V.
Se desarrolló un procedimiento por Cromatografía Líquida de Alta Resolución (HPLC) para determinar clortetraciclina en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con una solución de acetona ácida. El extracto se analizó por HPLC con una columna de fase reversa C18 y un detector UV (340 nm) con una mezcla de dimetilformamida al 27% como fase móvil. Los resultados de la validación mostraron una linealidad entre 50-1000 µg/mL que responde a una ecuación general de Y=0.23X-2.62 con un coeficiente de regresión de 0.996 (p&lt;0.05) y una variación del intervalo de confianza de la pendiente entre 0.23-0.24. El límite de detección y el de cuantificación fue de 33µg/g y 50 µg/g...
Tipo: Journal article
Palavras-chave: Clortetraciclina; HPLC; Pienso.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200009

DETERMINACIÓN DE AIVLOSIN EN PIENSOS POR CROMATOGRAFÍA LÍQUIDA DE ALTA RESOLUCIÓN (HPLC)
Provedor de dados: 101
Autores: Escobar,A; Abeledo,María Antonia; Vega,E; Cedeño,V.
Un procedimiento se desarrolló por cromatografía líquida de alta resolución (HPLC) para determinar Aivlosin en alimentos destinados a diferentes categorías productivas de la crianza del cerdo. La extracción del analito se realizó con acetonitrilo y se concentró cinco veces cuando fue inferior a 20 ppm. El extracto se analizó por HPLC con una columna de fase reversa C18, un detector UV (280 nm) y una mezcla de acetonitrilo: acetato de amonio 0.15M: ácido acético en una proporción de 45:45:10 v/v/v, como fase móvil. Los resultados de la validación mostraron una linealidad entre 5-80 µg/ml, que responde a una ecuación general de Y=-2.60+1.72X con un coeficiente de regresión de 0.999(p&lt;0.05) y una variación del intervalo de confianza de la pendiente entre...
Tipo: Journal article
Palavras-chave: Aivlosin; HPLC; Piensos.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200008

APLICACIÓN DEL HPLC EN EL ESTABLECIMIENTO DEL PERIODODE LATENCIA DE LA OXITETRACICLINA EN MÚSCULO DE CAMARÓN(Litopenaeus vannamei) EN UN SISTEMA DE PRODUCCIÓNSEMI-INTENSIVO EN CUBA
Provedor de dados: 101
Autores: Escobar,A; Faure,R; Sosa,Dayana; Betancourt,A; Hernández,Damaiky.
Se desarrolló una metodología para la determinación de oxitetraciclina en músculo de camarón por cromatografía líquida de alta resolución empleando columna de fase reversa y detección UV a 340 nm. La extracción se desarrolló con una solución Mcllvaine-EDTA y los extractos fueron purificados empleando columnas de extracción fase sólida (C18-500mg-6mL). El método presentó un recobrado entre 78.2±9.9 % con una precisión menor del 10% en las concentraciones de 200 y 400 ng/g, la linealidad mostró un coeficiente de correlación mayor de 0.99 y el límite de detección fue 105 ng/g, Se estableció el periodo de latencia de la oxitetraciclina en músculo de camarones (Litopenaeus vannamei) entre 14-20 días cuando se suministra el alimento medicamentado a una dosis de...
Tipo: Journal article
Palavras-chave: Oxitetraciclina; HPLC; Camarón; Periodo de latencia.
Ano: 2010
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000200005

ความสัมพันธ์ของฤทธิ์ต้านอนุมูลอิสระกับปริมาณ Tocopherol และ gamma aminobutyric acid (GABA) ในข้าวกล้องและข้าวกล้องงอก
Provedor de dados: 106
Autores: Pakinee Akkaravessapong; Suganya Wongpornchai; Sunanta Wongpiyachon; Kitsada Pitija; Angsutorn Wasusun; Panawan Suttiarporn.
Rice contains several kinds of bio-functional components. In this study, γ-aminobutyric acid (GABA) and Tocopherol, or Vitamin E were identified and their relationships to antioxidant activity using DPPH assay of both brown and pre germinated Thai rice varieties were studied. GABA are one of the two inhibitory neurotransmitters which make the neuron resistant to stimulation by excitatory neurotransmitters and inhibitor of some cancer cells activity whereas Tocopherol, or Vitamin E, which acts as antioxidant and improve blood cholesterol. Identification of GABA and Tocopherol in both brown and pre-germinated brown rice grown from different locations were conducted in eleven varieties, namely: Khao Dawk Mali 105, PTT1, CN1, RD31, Sang yod, RD6, Hom...
Tipo: PhysicalObject
Palavras-chave: Antioxidant activity; Rice varieties; Tocopherol; DPPH assay; Brown rice; Pre-germinated brown rice; GABA; Gamma aminobutyric acid; Vitamin e; Nutritive value; Chemical composition; High performance liquid chromatography; HPLC; ฤทธิ์ต้านอนุมูลอิสระ; กาบา; โทโคฟีรอล; พันธุ์ข้าว; ข้าวกล้อง; ข้าวกล้องงอก; วิตามินอี; โครงสร้างทางเคมี; การวิเคราะห์องค์ประกอบทางเคมี; คุณค่าทางโภชนาการ.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5713

การวิเคราะห์ปริมาณบีตา-แคโรทีนในผลปาล์มน้ำมันแบบรวดเร็ว
Provedor de dados: 106
Autores: Sirinapha Khongcharean; Weeraphan Sridokchan; Patcharin Tanya; Pornsiri Liengsakul; Ronarit Ritiron.
ผลปาล์มน้ำมันเป็นแหล่งบีตา-แคโรทีนทางธรรมชาติที่สำคัญ ยังไม่มีรายงานถึงปริมาณบีตา-แคโรตีนในพันธุ์ปาล์มน้ำมันต่างๆ ที่ปลูกในประเทศไทย รวมถึงการวิเคราะห์หาปริมาณบีตา-แคโรทีนแบบรวดเร็ว วัตถุประสงค์ของงานวิจัยเพื่อวิเคราะห์ปริมาณบีตา-แคโรทีน ในผลปาล์มน้ำมันพันธุ์ต่างๆ ใน 2 พื้นที่ปลูก ได้แก่ จังหวัดกาญจนบุรีและจังหวัดทางภาคใต้ของประเทศ การวิเคราะห์แบบรวดเร็วโดยวิธีสเปกโตรสโกปีแบบ NIR เทียบกับวิธีสเปกโตรสโกปีแบบ UV-Vis ซึ่งเป็นวิธีมาตรฐานที่ใช้ในการอ้างอิง เพื่อนำมาสร้างสมการเทียบมาตรฐานหาปริมาณบีตา-แคโรทีน ในตัวอย่างผลปาล์มน้ำมันสด พบค่าเฉลี่ยปริมาณบีตา-แคโรทีนในผลปาล์มน้ำมันตั้งแต่ 1.04 – 15.42 mg/100g fw ซึ่งแตกต่างกันอย่างมีนัยสำคัญทางสถิติตามพันธุ์ปาล์มน้ำมัน ค่าเฉลี่ยของบีตา-แคโรทีนในกลุ่มตัวอย่างทางใต้ของประเทศมีค่าสูงกว่าในจังหวัดกาญจนบุรี คือ 9.41 และ...
Tipo: PhysicalObject
Palavras-chave: Oil palm; Beta-carotene; NIR spectroscopy; Near Infrared spectroscopy; UV-Vis spectroscopic; Fast analysis; High performance liquid chromatography; HPLC; Calibration set; ปาล์มน้ำมัน; สายพันธุ์; บีต้าแคโรทีน; สารธรรมชาติ; การวิเคราะห์แบบรวดเร็ว; สมการเทียบมาตรฐาน; สเปกโตรสโกปี; เทคนิคโครมาโตกราฟีของเหลวสมรรถนะสูง; สารชีวโมกุล.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5753

Quantitative determination by HPLC of iridoids in the bark and latex of himatanthus sucuuba
Provedor de dados: 36
Autores: Silva,Jefferson Rocha de Andrade; Amaral,Ana Claudia Fernandes; Silveira,Cleoni Virgínio da; Rezende,Claudia Moraes; Pinto,Angelo Cunha.
The main iridoids from the bark and latex of Himatanthus sucuuba were isolated and characterised by spectroscopic methods. HPLC was used for the quantitative analyses of these iridoids and the chromatograms of bark and latex showed a similar iridoid composition. Both parts of the plant are used in folk medicine for the treatment of various ailments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apocynaceae; HPLC; Himatanthus sucuuba; Iridoids; Quantitative determination.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000100014

ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES
Provedor de dados: 37
Autores: OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda.
In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017

Antioxidant activity and total phenol content of blackberries cultivated in a highland tropical climate
Provedor de dados: 1
Autores: Guedes, Mayara Neves Santos; Pio, Rafael; Maro, Luana Aparecida Castilho; Lage, Fabíola Fonseca; Abreu, Celeste Maria Patto de; Saczk, Adelir Aparecida.
Blackberries are an important option for the diversification of fruit crops. However, there is currently no literature regarding plant cultivation in high-altitude tropical climates. Knowledge of the phenolic composition of blackberries is essential because variations in the levels of these components may exist between cultivars and may depend on environmental conditions. High performance liquid chromatography (HPLC) was used to evaluate the total phenol content of different blackberry cultivars (Arapaho, Brazos, Cainguangue, Cherokee, Choctaw, Comanche, Ébano, Guarani, Tupy and Xavante). Free radical scavenging activity in these cultivars was assayed using a DPPH test. The HPLC-UV chromatogram of blackberry fruit extracts at 280 nm revealed the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.01.03.00-8 total phenols; Free radicals; HPLC; Rubus spp. Fitotecnia.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/28413

Quantification of anti-nutritional factors and their correlations with protein and oil in soybeans
Provedor de dados: 42
Autores: BUENO,RAFAEL D.; BORGES,LEANDRO L.; GOD,PEDRO I.V. GOOD; PIOVESAN,NEWTON D.; TEIXEIRA,ARLINDO I.; CRUZ,COSME DAMIÃO; BARROS,EVERALDO G. DE.
ABSTRACT Soybeans contain about 30% carbohydrate, mainly consisting of non-starch polysaccharides (NSP) and oligosaccharides. NSP are not hydrolyzed in the gastrointestinal tract of monogastric animals. These NSP negatively affect the development of these animals, especially the soluble fraction. This work aimed to establish a method to quantify NSP in soybeans, using high performance liquid chromatography (HPLC), and to estimate correlations between NSP, oligosaccharides, protein and oil. Sucrose, raffinose + stachyose, soluble and insoluble NSP contents were determined by HPLC. Oil and protein contents were determined by near-infrared spectroscopy (NIRS). The soluble PNAs content showed no significant correlation with protein, oil, sucrose and raffinose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Carbohydrates; Anti-nutritional factors; Quantitative analysis; HPLC; NIR spectroscopy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100205

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.
Provedor de dados: 42
Autores: EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN.
Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621

Gestational diabetes: a risk of puerperal hypovitaminosis A?
Provedor de dados: 42
Autores: RESENDE,FERNANDA B.S.; LIRA,LARISSA Q. DE; GRILO,EVELLYN C.; LIMA,MAYARA S.R.; DIMENSTEIN,ROBERTO.
The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colostrum; Gestational diabetes; HPLC; Hypovitaminosis A; Postpartum.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100463

HPLC identification of isoniazid residues in bovine milk
Provedor de dados: 45
Autores: Leite,R.M.H.; Leite,R.C.; Lima,J.A.; Fóscolo,C.B.; Mota,P.M.P.C.; Lobato,F.C.F.; Lage,A.P.
The high pressure liquid chromatography (HPLC) was used for the identification of isoniazid (isonicotinic acid hydrazide) in the milk of cattle treated with a dose of 25 mg/kg/day in alternated days. The effect of milk pasteurization on the isoniazid residue concentration was also studied. The drug excretion presented a cyclic variation, with higher levels in the first day after administration (aa), a mean of 1104.48µg/l, and a decrease two days aa, with a mean of 104.12µg/l. Four days after the last administration of the drug it was not possible to identify residues of isoniazid in the milk of treated animals. Body weight and milk yield influenced the amount of the excreted drug, and pasteurization decreased (mean 47.07%) the concentration of isoniazid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Tuberculosis; Isoniazid; Milk; HPLC.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600018

Caseinomacropeptide index in UHT whole milk stored under different conditions of temperature and time
Provedor de dados: 45
Autores: Villanoeva,C.N.B.C.; Andrade,E.H.P.; Baffa Junior,J.C.; Souza,M.R.; Cerqueira,M.M.O.P.; Fonseca,L.M.; Penna,C.F.A.M.; Leite,M.O.
Caseinomacropeptide (CMP) index is a method used to detect adulteration of milk by addition of cheese whey, since CMP is a glycopeptide characteristic produced during cheesemaking, and soluble in the whey phase. The objective of this work was to evaluate the caseinomacropeptide index of UHT milk stored under different temperatures. Six batches of recently processed UHT milk were collected and stored under three temperatures (21ºC, 6ºC, and -12ºC) and analyzed by HPLC in the day of the milk collection (day 0) and at 30, 60, 90, and 120 days of storage. The experiment was run as a randomized block design with a 3x5 factorial arrangement, and the Student-Newman-Keuls (SNK) method was used as the post-hoc test (p = 0.05). There was a progressive increase of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: UHT whole milk; Caseinomacropeptide; HPLC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100039

Detection of cheese whey and caseinomacropeptide in fermented milk beverages using high performance liquid chromatography
Provedor de dados: 45
Autores: Andrade,E.H.P.; Souza,M.R.; Fonseca,L.M.; Penna,C.F.A.M.; Cerqueira,M.M.O.P.; Roza,T.; Seridan,B.; Resende,M.F.S.; Pinto,F.A.; Villanoeva,C.N.B.C.; Leite,M.O.
Cheese whey level and caseinomacropeptide (CMP) index of fermented milk beverages added with four levels of cheese whey (0, 10, 20, and 40%) and stored at 8-10oC for 0, 7, 14 and 21 days were determined by high performance liquid chromatography-gel filtration (HPLC-GF). Additionally, the interference of the starter culture and the storage time on the detection of cheese whey and CMP were investigated. Refrigerated storage up to 21 days did not affect (P&gt;0.05) cheese whey and CMP amounts in milk (0% of cheese whey) and in fermented milk beverages added with 10 and 20% of cheese whey (P&gt;0.05). However, cheese whey and CMP amounts were higher than expected (P&lt;0.05) in fermented milk beverages added with 40% of cheese whey and stored for 21 days.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermented milk beverages; Cheese whey; CMP; HPLC.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300959

Liberação intraocular de ofloxacina associada a lente de contato biossintética em ceratite bacteriana experimental em cães
Provedor de dados: 45
Autores: Cremonini,D.N.; Ranzani,J.J.T.; Cunha,M.L.R.S.; Tonin,F.G.; Brandão,C.V.S.; Messadeq,Y.
Avaliou-se a concentração de ofloxacina liberada por uma lente de contato de membrana de celulose biossintética, para tratamento de ceratite bacteriana experimental em cães, pela inoculação de Staphylococcus aureus intraestromal. Comparou-se o tratamento com a lente de contato biossintética impregnada com ofloxacina à terapia tópica convencional. Realizou-se avaliação microbiológica e dosagem de ofloxacina no humor aquoso por meio do método de cromatografia líquida de alto rendimento (HPLC). Houve diferença estatística na contagem de colônias bacterianas entre os olhos com ceratite e os demais grupos, no primeiro dia de coleta. O biomaterial, impregnado com ofloxacina, promoveu liberação gradual durante o período de avaliação, aos três e sete dias; no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Ceratite bacteriana; Membrana biossintética; Ofloxacina; HPLC.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400004

Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography
Provedor de dados: 45
Autores: Lasmar,M.M.; Leite,M.O.; Fonseca,L.M.; Souza,M.R.; Cerqueira,M.M.O.P.; Penna,C.F.A.M.; Couto,C.N.B.; Ferreira,J.M.
High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P&gt;0.05),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Raw milk; Bronopol; Cheese whey detection; HPLC.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600037

Acaricidal activity of leaves of Morus nigra against the cattle tick Rhipicephalus microplus
Provedor de dados: 45
Autores: Dantas,A.C.S.; Freire,D.P.; Souza,G.R.; Almeida,J.R.G.S.; Rolim,L.A.; Castro,R.N.; Horta,M.C.
ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tick control; Acaricide; Plant extract; Mulberry; HPLC.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300523

Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments
Provedor de dados: 47
Autores: Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan.
Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Salicylic acid; UV-B.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006

Growth, delayed fluorescence and pigment composition of four Prorocentrum minimum strains growing at two salinities
Provedor de dados: 48
Autores: BERDEN-ZRIMEC,MAJA; FLANDER-PUTRLE,VESNA; DRINOVEC,LUKA; ZRIMEC,ALEXIS; MONTI,MARINA.
Prorocentrum minimum is a potentially harmful and widely distributed marine dinoflagellate. Several P. minimum strains have already been studied, showing phylogenetical relations of strains isolated from the same geographical regions. Similarity among the strains was further examined on the basis of their physiology. Pigment composition and concentration, as well as delayed fluorescence (DF) decay kinetics and intensity, were measured in four P. minimum strains isolated from the Baltic and Adriatic Seas. The strains were grown at two salinities characteristic of the Baltic (8 PSU) and North Adriatic Seas (32 PSU). Strain differences in DF decay kinetics and growth did not always follow their genetic relations. While two strains showed similarities to the...
Tipo: Journal article
Palavras-chave: Adriatic Sea; Baltic Sea; Delayed fluorescence; HPLC; Prorocentrum minimum; Strains.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100003

Phenolic compounds and vitamins in wild and cultivated apricot (Prunus armeniaca L.) fruits grown in irrigated and dry farming conditions
Provedor de dados: 48
Autores: Kan,Tuncay; Gundogdu,Muttalip; Ercisli,Sezai; Muradoglu,Ferhad; Celik,Ferit; Gecer,Mustafa Kenan; Kodad,Ossama; Zia-Ul-Haq,Muhammad.
BACKGROUND: Turkey is the main apricot producer in the world and apricots have been produced under both dry and irrigated conditions in the country. In this study, phenolic compounds and vitamins in fruits of one wild (Zerdali) and three main apricot cultivars ('Cataloglu', 'Hacihaliloglu' and 'Kabaasi') grown in both dry and irrigated conditions in Malatya provinces in Turkey were investigated. RESULTS: The findings indicated that higher content of phenolic compounds and vitamins was found in apricot fruits grown in irrigated conditions. Among the cultivars, 'Cataloglu' had the highest rutin contents both in irrigated and dry farming conditions as 2855 µg in irrigated and 6952 µg per 100 g dried weight base in dry conditions and the highest chlorogenic...
Tipo: Journal article
Palavras-chave: Apricot; Phytochemicals; Ripening; Irrigation; HPLC.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100046

Altered dopamine levels induced by the parasite Profilicollis antarcticus on its intermediate host, the crab Hemigrapsus crenulatus
Provedor de dados: 48
Autores: ROJAS,JOSÉ MIGUEL; OJEDA,F. PATRICIO.
A serotonergic pathway is apparently involved in parasite-host interactions. Previous studies conducted in our laboratory showed increased rates in oxygen consumption and alterations in body posture in the crab Hemigrapsus crenulatus parasitized by the acanthocephalan, Profilicollis antarcticus. Such changes may be related to the functions described for biogenic amines in crustaceans. During the infective stage the acanthocephalans live freely in the hemocelomic cavity, suggesting that the possible alteration induced by biogenic amines may be related to their neurohormonal function in crustaceans. To test whether the presence of P. antarcticus produced neurohormonal changes in its intermediate host, H. crenulatus, we analyzed serotonin and dopamine levels...
Tipo: Journal article
Palavras-chave: Acanthocephalans; Biogenic amines; HPLC; Parasite-host interaction; Parasite transmission.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200015

In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica
Provedor de dados: 48
Autores: Moukette,Bruno Moukette; Pieme,Constant Anatole; Njimou,Jacques Romain; Biapa,Cabral Prosper Nya; Marco,Bravi; Ngogang,Jeanne Yonkeu.
BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose...
Tipo: Journal article
Palavras-chave: Antioxidant; Radical scavenging; HPLC; Monodora myristica; Non-timber forest product.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100015

Sugars, organic acids, and phenolic compounds of ancient grape cultivars (Vitis vinifera L.) from Igdir province of Eastern Turkey
Provedor de dados: 48
Autores: Eyduran,Sadiye Peral; Akin,Meleksen; Ercisli,Sezai; Eyduran,Ecevit; Maghradze,David.
BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz....
Tipo: Journal article
Palavras-chave: Table grape; Biochemical composition; HPLC; Spectrophotometer; Germplasm characterization.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100002

Stability of unpasteurized and refrigerated orange juice
Provedor de dados: 52
Autores: Souza,Maria Cristina Corrêa de; Benassi,Marta de Toledo; Meneghel,Renata Fraxino de Almeida; Silva,Rui Sérgio dos Santos Ferreira da.
The stability of orange juice obtained from a small extractor and stored in a polyethylene bottle was assessed under isothermal and non-isothermal storage conditions at 4, 8 and 12ºC for 72 hours. pH, titratable acidity and Brix did not alter significantly during the 72 hours storage. Microbiological analysis showed high initial count for moulds and yeasts that increased in the juice stored for 72h under the non-isothermal conditions with temperature abuse (12ºC/4h). Date of the sensory evaluation showed a small reduction in product acceptance in this condition. The juice, in the recommended validity period (48h), presented losses of less than 20% of the initial ascorbic acid content regardless of the treatment. However, after this time, the degradation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus fruit; Shelf life; Vitamin C; HPLC; Sensory quality; Moulds and yeasts.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300009

Production of specific polyclonal antibodies anti-human factor IX
Provedor de dados: 52
Autores: Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010

Determination of 4-vinylgaiacol and 4-vinylphenol in top-fermented wheat beers by isocratic high performance liquid chromatography with ultraviolet detector
Provedor de dados: 52
Autores: Zhu,Mingguang; Cui,Yunqian.
This work aimed at determining 4-vinylguaiacol and 4-vinylphenol in the top-fermented wheat beers using different wavelength and the mobile phase for HPLC. Best results for isocratic elution were obtained at 260 nm and the mobile phase comprising methanol/ultrapure water/phosphoric acid (400/590/10, V/V). Under these conditions, the retention time of 4-vinylguaiacol and 4-vinylphenol was 25 and 27min, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 4-vinylguaiacol; 4-vinylphenol; Top-fermented wheat beers; HPLC; Phenolic off-flavor.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600018

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee
Provedor de dados: 52
Autores: Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020

Identification and quantification of monomers released from dental composites using HPLC
Provedor de dados: 52
Autores: Archegas,Lucí Regina Panka; Rached,Rodrigo Nunes; Ignacio,Sérgio Aparecido; Vasconcelos,Eliane Carvalho de; Ramos,Débora Toledo; Souza,Evelise Machado de.
The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250) and immersed in deionized water at 37ºC for 28 days, with water changes in 1, 7, 14 and 21 days. The mean concentration of residual monomers were subject to the Kruskal-Wallis test (p&lt;0.05). Tetric Ceram exhibited significantly higher concentrations of leached monomers. Bis-GMA was the monomer released in lower concentrations for all the materials. There was no statistical difference between the amounts of TEGDMA and UDMA. Most of the monomers demonstrated maximal concentration at the 7-day period. The HPLC analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dental composites; Monomers; HPLC; TEGDMA; Bis-GMA; UDMA.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400009

Extraction time for soybean isoflavone determination
Provedor de dados: 52
Autores: Carrão-Panizzi,Mercedes C.; Favoni,Silvana Pedroso de Goés; Kikuchi,Akio.
Studies were carried out on the optimization of the extraction procedures for isoflavones determination in soybean by HPLC. Isoflavones were extracted from 100 mg samples of full fat soybean flour "Kinako" (milled toasted grains). Recovery of average total isoflavones was higher when extraction was performed with agitation(217.2 mg/100g), than without agitation(191.0 mg/100g). Isoflavone extraction without agitation for 1,4, 20 and 24 hours, were equally efficient for total and individual compounds. These results suggested that an efficient isoflavone recovery could be achieved with extraction for one hour with agitation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybeans; Isoflavones; Glucosides; Aglucones; Extraction; Ethanol; HPLC.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600015

Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents
Provedor de dados: 52
Autores: Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos.
The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001

Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31
Provedor de dados: 58
Autores: Todorov,Svetoslav D.; Vaz-Velho,Manuela; Gibbs,Paul.
Two methods of purification of the plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31 are used in this study - the method of ammonium sulfate precipitation, Sep-pack C18 cartridge and reverse-phase HPLC chromatography on C18 Nucleosil column, and the method of direct purification by cation exchange SP Sepharose Fast Flow column Amersham (Pharmacia Biotech). The purity of the products from the two experimental protocols are examined for their molecular weight, aminoacid composition and sequence. Comparison of results show that the plantaricins purified with the two methods are identical. Both methods may be used to purify plantaricin ST31. Comparison of the yield in the purification protocols is 0.8% in the HPLC experimental protocol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus plantarum; Bacteriocin; Plantaricin; HPLC; Cation-exchange chromatography (IEX).
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100026

Occurrence of aflatoxin B1 in natural products
Provedor de dados: 58
Autores: Prado,Guilherme; Altoé,Aline F.; Gomes,Tatiana C. B.; Leal,Alexandre S.; Morais,Vanessa A. D.; Oliveira,Marize S.; Ferreira,Marli B.; Gomes,Mateus B.; Paschoal,Fabiano N.; Souza,Rafael von S.; Silva,Daniela A.; Madeira,Jovita E. G. Cruz.
The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Fungi; Mycotoxin; High-performance liquid chromatography; HPLC.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026

Regional analysis of potential polychlorinated biphenyl degrading bacterial strains from China
Provedor de dados: 58
Autores: Shuai,Jianjun; Yu,Xurun; Zhang,Jing; Xiong,Ai-sheng; Xiong,Fei.
ABSTRACT Polychlorinated biphenyls (PCBs), the chlorinated derivatives of biphenyl, are one of the most prevalent, highly toxic and persistent groups of contaminants in the environment. The objective of this study was to investigate the biodegradation of PCBs in northeastern (Heilongjiang Province), northern (Shanxi Province) and eastern China (Shanghai municipality). From these areas, nine soil samples were screened for PCB-degrading bacteria using a functional complementarity method. The genomic 16S rDNA locus was amplified and the products were sequenced to identify the bacterial genera. Seven Pseudomonas strains were selected to compare the capacity of bacteria from different regions to degrade biphenyl by HPLC. Compared to the biphenyl content in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polychlorinated biphenyls; Biphenyl; Biodegradation; Pseudomonas sp.; HPLC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300536

Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens
Provedor de dados: 58
Autores: Mahmoud,D.A; Hassanein,N.M; Youssef,K.A; Abou Zeid,M.A.
This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was purified and chemically...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azadirachta indica; Aqueous and organic extracts; HPLC; Fungal inhibitory effect.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300021

Developed and validated stability indicating HPLC method for the determination of epirubicin in bulk drug, marketed injection and polymeric nanoparticles
Provedor de dados: 60
Autores: Tariq,Mohammad; Thomas,Shindu; Singh,Anu; Talegaonkar,Sushama.
Present work is aimed to develop a simple, sensitive, robust and reliable HPLC method for routine quality control of epirubicin (EPI) in bulk drug, marketed injections and polymeric nanoparticles. Separation was carried out by C18 column. Isocratic elution was carried out using mobile phase A: 0.16% o-phosphoric acid solution, B: acetonitrile and methanol mixture (80:20, v/v) in the ratio of 60:40 (A: B) while the flow rate was maintained at 1mL/min. Analyses were performed at 233.5 nm using PDA detector. Excellent linear relationship was observed between peak-area versus drug concentration in the range of 1.0-100.0 μg/mL (r2, 0.999). Developed method was found to be sensitive (Limits of detection and quantification were found to be ~8 ng/mL and ~25 ng/mL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epirubicin; HPLC; Polymeric nanoparticles; Quality control; Forced degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400619

Antidiabetic activity of aqueous extract of Sigesbeckia orientalis (St. Paul’s Wort) in alloxan-induced diabetes model
Provedor de dados: 60
Autores: Asif,Muhammad; Saleem,Mohammad; Yousaf,Sobia; Saadullah,Malik; Zafar,Memoona; Khan,Rizwan Ullah; Yuchi,Alamgeer.
The current study evaluated antidiabetic and antihyperlipidemic activities of aqueous extract of Sigesbeckia orientalis L. (St. Paul’s Wort) (AESO) in an alloxan-induced diabetic rat model. Following OECD guidelines safe doses of AESO were assessed in rats for the main study. Serum/bood glucose, α-amylase, and lipids levels and histopathological evaluations were conducted to assess antidiabetic and associated antihyperlipidemic efficacies of AESO. AESO was found to be safe up to the dose of 2000 mg/kg. Significant (p &lt; 0.05) reduction in glucose and lipids (total cholesterol, triglycerides, low-density lipoproteins) levels was observed in AESO treatment groups. Serum α-amylase, high-density lipoproteins, and total body weight was increased significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antidiabetic; Sigesbeckia orientalis L; Alloxan; Antihyperlipidemic; HPLC.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100607

RP-HPLC method development and validation for estimation of rivaroxaban in pharmaceutical dosage forms
Provedor de dados: 60
Autores: Çelebier,Mustafa; Reçber,Tuba; Koçak,Engin; Altinöz,Sacide.
Rivaroxaban, an anti-clotting medication, acts at a crucial point in the blood-clotting process and stops the formation of blood clots. In this study, RP-HPLC method was developed for the determination of rivaroxaban in tablets (Xarelto® (10 mg)). Phenomenex Luna 5 µm C18 100 Å LC Column (250 x 4.6 mm) was used at 40 ºC. Isocratic elution was performed with ACN:Water (55:45 v/v) mixture. The flow rate was 1.2 mL min-1 and UV detection was at 249 nm. Internal standard (Caffeine) and rivaroxaban were eluted within 2.21 and 3.37 minutes, respectively. The developed method was validated according to the ICH guidelines and found to be linear within the range 0.005 - 40.0 µg mL-1. The method was accurate, precise, robust and rapid. Thus, it was applied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPLC; Rivaroxaban; Validation; System suitability; Stability-indicating; Pharmaceutical dosage form.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200018

Determination of apramycin in oral soluble powder by a HPLC method using pre-column derivatization with o-phthalaldehyde and UV detection
Provedor de dados: 60
Autores: Antunes,Elisabete de Almeida Barbosa; Lourenço,Felipe Rebello; Pinto,Terezinha de Jesus Andreoli.
A high-performance liquid chromatographic method employing pre-column derivatization with o-phthalaldehyde (OPA) and 2-mercaptoacetic acid was developed for the determination of apramycin, an aminoglycoside antibiotic used in veterinary medicine, in the oral soluble powder form. The chromatographic separation was done by ion-pair HPLC using a C18 reversed-phase column, Synergy Hydro (150 mm x 4.6 mm x 4 µm) and mobile phase composed of 0.005 mol/L sodium octanosulfonate in a mixture of acetonitrile: water: acetic acid (45:55:2) (v/v/v) with a flow rate of 1.0 mL/min; the UV detector was operated at 332 nm. The developed method was validated according to official compendia guidelines, having demonstrated robustness, selectivity and linearity for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apramycin; HPLC; Microbiological Assay; Pre-column Derivatization; OPA.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200007

Determination of phytoconstituents of n-hexane extract of leaves of Morus nigra and evaluation of their effects on biochemical and histopathological parameters in paracetamol intoxicated mice liver
Provedor de dados: 60
Autores: Mallhi,Tauqeer Hussain; Qadir,Muhammad Imran; Khan,Yusra Habib.
In-vitro investigation of Morus nigra L. (Moraceae) has demonstrated the evidence of several antioxidant compounds. Current study was aimed to determine the phytoconstituents and hepatoprotective potential of n-hexane extract of Morus nigra. Swiss albino mice were divided into five groups: normal control, paracetamol control, silymarin control, 250 mg/kg p.o. extract for 7 days plus paracetamol 3 h later, 500 mg/kg p.o. extract for 7 days plus paracetamol 3 h later. Serum levels of liver enzymes and total bilirubin were assessed and compared between the groups by using one-way ANOVA to confirm hepatoprotective activity. HPLC analysis revealed the presence quercetin, oleanolic acid, luteolin, apigenin, vitamin C and kuwanon C. n-hexane extract of Morus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moraceae; HPLC; Quercetin; Morus nigra/antioxidant; Morus nigra/extract/hepatoprotective.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300631

Determination of Gemifloxacin in human plasma by high performance liquid chromatography using Ultra Violet detector and its application to a bioequivalence study
Provedor de dados: 60
Autores: Mousavi,Syed Husain Hashemi.
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610

Development and validation of an HPLC-UV method for accelerated stability study and pharmacokinetic analysis of venlafaxine
Provedor de dados: 60
Autores: Sher,Muhammad; Ahmad,Maria; Hassan,Faiza; Naeem-ul-Hassan,Muhammad; Hussain,Muhammad Ajaz.
A reverse phase high performance liquid chromatography method has been developed and validated for accelerated stability study and determination of pharmacokinetic parameters of venlafaxine HCl. The chromatographic separation was carried out using ODS analytical column (250 × 4.6 mm i.d., 5 µm particle size). The mobile phase included acetonitrile, methanol and potassium dihydrogen phosphate buffer (30:30:40; pH 6.1) at a flow rate 1.5 mL min−1. UV-Visible detector was used at wavelength of 227 nm to monitor elutions. Retention time observed was 2.745 min. The method was validated for linearity, accuracy, precision, sensitivity and robustness. Accelerated stability study of venlafaxine HCl capsules was carried out at 40 and 50 ºC under 75% RH level....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venlafaxine; HPLC; Stability; Pharmacokinetics; Chromatography.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100514

Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria)
Provedor de dados: 60
Autores: Nassima,Boumghar; Nassima,Behidj; Riadh,Ksouri.
The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of Tizi-Ouzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Populus nigra; Populus alba; HPLC; Polyphenols; Antimicrobial activity; Antibiofilm activity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100563

Effect on hyperglycemia and pancreas cells of chaya aqueous extracts from two different regions in streptozotocin-induced diabetes rats
Provedor de dados: 60
Autores: Guzmán,Eduardo Lozano; González,José Cruz Calderón; Flores,Maribel Cervantes; Carrillo,Ada Sandoval; Pescador,María Guadalupe Nieto; Cruz,Francisco Javier Moreno.
Cnidoscolus chayamansa is a native plant of the Mayan region, which is also cultivated in other places like northern Mexico, Tunisia and India. Many properties are attributed to Mayan Chaya, such as aid in the control of glycemia in diabetics. Thus this study aimed to evaluate the hypoglycemic effects of chaya aqueous extracts in a model of streptozotisin-induced diabetic Wistar rats. Chaya aqueous extracts were collected from plants cultivated in Quinta Roo (Mayan region) and Durango (northern Mexico), and in this study we compare their effect with metformin (as a control). Additionally, we compared the extracts mass profiles from both regions by high-resolution liquid chromatography coupled to a triple quadrupole tandem mass detector (HPLC-MS/MS QQQ)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Chaya; Langerhans islets; Hypoglycemia; HPLC.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100619

Stability-indicating HPLC-DAD method for the simultaneous determination of fluoroquinolone in combination with a non-steroidal anti-inflammatory drug in pharmaceutical formulation
Provedor de dados: 60
Autores: Araujo,Elaine de Oliveira; Rosa,Aline Marques; Amaral,Marcos Serrou do; Sversut,Rúbia Adrieli; Baroni,Adriano César de Morais; Oliveira,Lincoln Carlos Silva de; Kassab,Nájla Mohamad.
We developed and validated a stability-indicating assay method for the simultaneous determination of enrofloxacin and piroxicam in combination and in the presence of degradation products. Reverse-phase high-performance liquid chromatography analyses were carried out on a Vertisep C18 column and acetonitrile-water (48:52 v/v, pH 3.0) mobile phase with a 1.00 mL min−1 flow rate. The efficient chromatographic separation of these drugs and their forced degradation products was achieved in less than 5min with a peak purity match factor higher than 950. The method used showed linearity in the concentration ranges of 0.25 to 16.0 µg mL−1 for enrofloxacin (r = 0.9997) and 0.125 to 8.0 µg mL−1 for piroxicam (r = 0.9999) as well as precision (relative standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enrofloxacin; HPLC; Piroxicam; Simultaneous determination; Stability-indicating method.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100515

Postharvest Sensory and Phenolic Characterization of ‘Elegant Lady’ and ‘Carson’ Peaches
Provedor de dados: 117
Autores: Infante,Rodrigo; Contador,Loreto; Rubio,Pía; Aros,Danilo; Peña-Neira,Álvaro.
High quality fruits are increasingly demanded along with the need to ensure this attribute to consumers. Thus, this study was aimed at characterizing a melting (‘Elegant Lady’) and a non-melting flesh (‘Carson’) peach (Prunus persica (L.) Batsch) varieties by considering both their bioactive compound contribution and their sensory quality in ripe fruit at harvest and after a prolonged period of cold storage. Cultivars were evaluated at harvest (F0) and after 30 d of cold storage (F30), as well as after a maturation period at 20 °C for both F0 and F30 (R0 and R30, respectively). Fruit weight, flesh firmness, soluble solid content (SSC), titratable acidity (TA), and background color (Hue) were recorded at each stage. Furthermore, total phenol content was...
Tipo: Journal article
Palavras-chave: Quality parameters sensory evaluation total phenols (+)-catechin; HPLC.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300016

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst
Provedor de dados: 117
Autores: Cartes Riquelme,Pricila; Ríos Leal,Darcy; Sáez Carrillo,Kátia; Uribe Moraga,Matilde; Valenzuela Aguilar,Sofía; Joseph Bolus,Stephen; Sánchez Olate,Manuel.
Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. &amp;amp; Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification....
Tipo: Journal article
Palavras-chave: Somatic embryogenesis; Maturation; Germination; HPLC.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007

Nutritional value of non-conventional vegetables prepared by family farmers in rural communities
Provedor de dados: 65
Autores: Oliveira,Heliane Aparecida Barros de; Anunciação,Pamella Cristine; Silva,Bárbara Pereira da; Souza,Ângela Maria Natal de; Pinheiro,Soraia Silva; Lucia,Ceres Mattos Della; Cardoso,Leandro de Morais; Castro,Luiza Carla Vidigal; Pinheiro-Sant’Ana,Helena Maria.
ABSTRACT:  Four most consumed non-conventional vegetables were analyzed raw and after cooking techniques routinely used by family farmers: ora-pro-nobis (Pereskia aculeata Mill.); wild mustard (Sinapis arvensis L.), serralha (Sonchus arvensis L), and capiçova (Erechtites valeriana). Chemical composition was determined according to AOAC. Vitamin C, vitamin E and carotenoids were determined by high-performance liquid chromatography, and phenolic compounds and minerals by spectrophotometry. Vitamin E and carotenoids concentrations were higher in stir fried wild mustard (7.68 mg.100 g-1 and 7.45 mg.100 g-1, respectively). Cooking reduced some minerals concentration in the non-conventional vegetables, but increased vitamins and carotenoids concentrations. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamins; HPLC; Agroecology; Food sovereignty.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800900

Lixiviação do imazethapyr em solo de várzea sob dois sistemas de manejo
Provedor de dados: 65
Autores: Kraemer,Alejandro Fausto; Marchesan,Enio; Grohs,Mara; Avila,Luis Antonio de; Machado,Sérgio Luiz de Oliveira; Zanella,Renato; Massoni,Paulo Fabricio Sachet; Sartori,Gerson Meneghetti Sarzi.
O imazethapyr está sendo amplamente utilizado no Rio Grande do Sul desde o desenvolvimento da Tecnologia Clearfield® (CL) em arroz, em uma mistura formulada de imazethapyr + imazapic (75 + 25g L-1). No entanto, com o uso dessa tecnologia, surgiu o problema da persistência do herbicida no solo, afetando cultivos não tolerantes em sucessão com diferentes intensidades. A fitointoxicação está relacionada, dentre vários fatores, à localização do herbicida no perfil do solo. O presente trabalho teve por objetivo determinar o posicionamento do imazethapyr em profundidade, no perfil de um solo de várzea cultivado com arroz, frente a dois tipos de manejo. Foram coletadas amostras de solos, em diferentes profundidades (0-5, 5-10, 10-15 e 15-20cm), do solo de várzea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Percolação de herbicidas; Residual; Preparo de solo; HPLC; Oryza sativa.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600005