Consulta: subjectFacets:"Haplotypes"
Registros recuperados: 18
Data/hora: 11/06/2024 13:25:04

Single nucleotide polymorphisms in partial sequences of the gene encoding the large sub-units of ADP-glucose pyrophosphorylase within a representative collection of 10 Musa genotypes
Provedor de dados: 69
Autores: Mahendhiran,Muhilan; Ramirez-Prado,Jorge Humberto; Escobedo-Gracia Medrano,Rosa Maria; Canto-Canché,Blondy; Tzec-Simá,Miguel; Grijalva-Arango,Rosa; James-Kay,Andrew.
Background ADP-glucose pyrophosphorylase (AGPase) is a rate-limiting enzyme catalyzing the first step in the starch biosynthesis pathway in higher plants. To date, there are no reported variants or isoforms of the AGPase enzyme in bananas (Musa spp. family Musaceae) as is the case of other plants. In this study, genomic DNA sequences homologous to the gene encoding one of the large subunits of the enzyme were amplified from 10 accessions of the genus Musa, including representatives of wild ancestors (AA and BB genomes), dessert bananas (AA, AAA, AB and AAB genomes), plantains (AAB genome) and cooking bananas (ABB and AAA genomes), and studied in order to find single nucleotide polymorphisms (SNP) base variations in Musa accessions. Results In the 810-base...
Tipo: Journal article
Palavras-chave: Haplotypes; Starch metabolism enzyme; Variants.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300006

PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: 74
Autores: Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (‘pinheiro-do-Paraná’ in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020

CYP1A1 genotypes and haplotypes and risk of oral cancer: a case-control study in South Indians
Provedor de dados: 74
Autores: Balaji,Lakshmi; Singh,Krishna Balaji; Bhaskar,Lakkakula V.K.S.
The CYP1A1 gene encodes for the enzyme, aryl hydrocarbon hydroxylase, which is involved in the biotransformation of various aromatic tobacco precarcinogens. In the present study, the association between CYP1A1 gene polymorphisms (IVS1-728G &gt; A, Thr461Asn and Ile462Val), and the risk of oral cancer, was examined among 157 patients with oral cancer and 132 age-matched controls, in a south Indian population. The strength of the association between CYP1A1 variants and oral cancer was estimated by logistic regression. It was found that Thr461Asn was not polymorphic. Both IVS1-728G &gt; A and Ile462Val frequencies were consistent with Hardy-Weinberg equilibrium in the control group. There were no significant differences in genotype or haplotype frequencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CYP1A1; Haplotypes; Allele; Oral cancer.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300004

Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome
Provedor de dados: 74
Autores: Tiwari,Santosh K.; Sharma,Vishwas; Sharma,Varun Kumar; Gopi,Manoj; Saikant,R; Nandan,Amrita; Bardia,Avinash; Gunisetty,Sivaram; Katikala,Prasanth; Habeeb,Md. Aejaz; Khan,Aleem A.; Habibullah,C.M.
The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Helicobacter pylori; EPIYA motifs; Tyrosine phosphorylation motifs; Haplotypes; Anatomically modern humans.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019

Beta-globin gene cluster haplotypes in Venezuelan sickle cell patients from the State of Aragua
Provedor de dados: 74
Autores: Moreno,Nancy; Martínez,José A.; Blanco,Zorella; Osorio,Leidys; Hackshaw,Patrick.
Seven polymorphic sites in the beta-globin gene cluster were analyzed on a sample of 96 chromosomes of Venezuelan sickle cell patients from the State of Aragua. The Benin haplotype was predominant with a frequency of 0.479, followed by the Bantu haplotype (0.406); a minority of cases with other haplotypes was also identified: atypical Bantu A2 (0.042), Senegal (0.031), atypical Bantu A7 (0.021) and Saudi Arabia/Indian (0.021) haplotypes; however, the Cameroon haplotype was not identified in this study. Our results are in agreement with the historical records that establish Sudanese and Bantu origins for the African slaves brought into Venezuela.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Beta-globin gene cluster; Sickle cell.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100005

β-globin haplotypes in normal and hemoglobinopathic individuals from Reconcavo Baiano, State of Bahia, Brazil
Provedor de dados: 74
Autores: Silva,Wellington dos Santos; Klautau-Guimarães,Maria de Nazaré; Grisolia,Cesar Koppe.
Five restriction site polymorphisms in the β-globin gene cluster (HincII-5'ε, HindIII-Gγ, HindIII-ªγ, HincII-'ψβ1 and HincII-3''ψβ1) were analyzed in three populations (n = 114) from Reconcavo Baiano, State of Bahia, Brazil. The groups included two urban populations from the towns of Cachoeira and Maragojipe and one rural Afro-descendant population, known as the "quilombo community", from Cachoeira municipality. The number of haplotypes found in the populations ranged from 10 to 13, which indicated higher diversity than in the parental populations. The haplotypes 2 (+----),3(----+),4(-+--+)and6(-++-+)onthe βA chromosomes were the most common, and two haplotypes, 9 (-++++)and 14 (++--+), were found exclusively in the Maragojipe population. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Afro-descendant; Β-globin; Haplotypes; Hemoglobinopathies.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300002

alpha-thalassemia, HbS, and beta-globin gene cluster haplotypes in two Afro-Uruguayan sub-populations from northern and southern Uruguay
Provedor de dados: 74
Autores: Luz,Julio A. da; Sans,Mónica; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins; Sonati,Maria de Fatima; Costa,Fernando Ferreira.
Hemoglobinopathies are the most common monogenic disorders worldwide; however, they have never been systematically studied from a genetic perspective in Uruguay. In this study, we determined the frequencies of hemoglobin variants in Afro-Uruguayans. A sample of 52 healthy unrelated Afro-Uruguayans from the northern (N = 28) and southern (N = 24) regions of the country was analyzed. Eight individuals (15.4%) were heterozygous for -alpha3,7thalassemia; seven of them (29.2%) were originally from the southern region, whereas one of them (3.6%) was from the northern region; the differences between both regions were statistically significant (p = 0.016 +/-0.003). The only structural mutation detected was betaS, which is typical of African populations. Four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemoglobinopathies; Alpha-thalassemias; HbS; Haplotypes; Afro-derived populations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400002

Determination of βS haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil
Provedor de dados: 74
Autores: Cabral,Cynthia Hatsue Kitayama; Serafim,Édvis Santos Soares; Medeiros,Waleska Rayane Dantas Bezerra de; Fernandes,Thales Allyrio Araújo de Medeiros; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sonati,Maria de Fátima; Rebecchi,Ivanise Marina Moretti; Medeiros,Tereza Maria Dantas de.
βS haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Β-globin; Sickle-cell anemia; Brazilian population; S hemoglobin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300009

A la recherche de l'huître portuguaise: identification de marqueurs moléculaires discriminant Crassostrea angulata et Crassostrea gigas
Provedor de dados: 5
Autores: Cornette, Florence.
L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR...
Tipo: Text
Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes.
Ano: 1996
URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf

Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae)
Provedor de dados: 16
Autores: Boluda, C.G.; Rico, V.J.; Divakar, P.K.; Nadyeina, O.; Myllys, L.; McMullin, R.T.; Zamora, J.C.; Scheidegger, C.; Hawksworth, D.L.
In many lichen-forming fungi, molecular phylogenetic analyses lead to the discovery of cryptic species within traditional morphospecies. However, in some cases, molecular sequence data also questions the separation of phenotypically characterised species. Here we apply an integrative taxonomy approach ‒ including morphological, chemical, molecular, and distributional characters ‒ to re-assess species boundaries in a traditionally speciose group of hair lichens, Bryoria sect. Implexae. We sampled multilocus sequence and microsatellite data from 142 specimens from a broad intercontinental distribution. Molecular data included DNA sequences of the standard fungal markers ITS, IGS, GAPDH, two newly tested loci (FRBi15 and FRBi16), and SSR frequencies from 18...
Tipo: Article / Letter to the editor
Palavras-chave: Chemotypes; Cryptic species; Haplotypes; Incomplete lineage sorting; Integrative taxonomy; Microsatellites; Speciation; Species concepts.
Ano: 2019
URL: http://www.repository.naturalis.nl/record/660277

Populations analysis of the Brazilian Sharpnose Shark Rhizoprionodon lalandii (Chondrichthyes: Carcharhinidae) on the São Paulo coast, Southern Brazil: inferences from mt DNA sequences
Provedor de dados: 83
Autores: Mendonça,Fernando Fernandes; Oliveira,Claudio; Gadig,Otto Bismarck Fazzano; Foresti,Fausto.
Sharks of the genus Rhizoprionodon can be considered some of the most important predators along the trophic coastal marine ecosystems and represent an important economic resource for the small-scale fisheries, especially on the Brazilian coastline. In order to analyze the population structure of the shark Rhizoprionodon lalandii of São Paulo, Southeastern coast of Brazil, levels of genetic diversity were identified by nucleotide sequence analyses of the mitochondrial DNA control region. The results obtained from this study present moderate values of haplotype diversity and low nucleotide diversity. Although the AMOVA tests (ΦST = 0.08394, P &lt; 0.01) had shown slightly differences among the studied samples, evidence for the occurrence of population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-loop; Haplotypes; Mitochondrial control region; Shark conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000200012

Stranded humpback whale (Megaptera novaeangliae) (Cetacea: Balaenopteridae) in Paraná River Delta, Buenos Aires Province, Argentina. Comments on the occurrence of marine mammals in the La Plata River Basin
Provedor de dados: 84
Autores: Lucero,Sergio O.; Gariboldi,María Constanza; Bauni,Valeria; Meluso,Juan Manuel; Castillo,Daniela del; Agnolin,Federico L.; Bogan,Sergio.
Abstract The humpback whale (Megaptera novaeangliae) is distributed among most oceans and seas of the globe (except Mediterranean Sea). These whales migrate from feeding regions in the Antarctic waters to breeding areas in tropical and subtropical seas. Here we report the stranding of a female young humpback whale, which was founded dead in the vicinity of the Talavera Island, in the Paraná River Delta, Buenos Aires Province, Argentina. From the analysis of mitochondrial cytochrome c oxidase subunit I gene sequences, two novel haplotypes were found, totalizing four haplotypes described for the species. In the La Plata River Basin this species was found only twice at the end of the XIX century. Thus, the new finding constitutes an important addition to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Megaptera novaeangliae; Humpback whale; Delta del Río Paraná; Haplotypes; Argentina.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492018000100200

Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops
Provedor de dados: 86
Autores: Queiroz,Paulo Roberto; Ramiro,Carolina Almeida; Martins,Érica Soares; Soberón,Mário; Bravo,Alejandra; Monnerat,Rose Gomes.
Abstract: The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gossypium hirsutum; Zea mays; Fall armyworm; Haplotypes; ND1 gene; RAPD.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500692

Exploring Parthenium weed biotypes by chloroplast DNA barcode analysis
Provedor de dados: 87
Autores: Anjum,Tehmina; Jabeen,Rasheda; Adkins,Steve; Akram,Waheed.
Abstract  Background:  Parthenium weed (Parthenium hysterophorus L.) is an invasive weed that has invaded vast regions of Pakistan in a relatively very short period of a decade or two, threatening the crop fields of the agrarian fed country. Parthenium hysterophorus L. is native of central South America and Gulf of Mexico, has now turned out to be a weed of global significance due to its alarming invasions and profuse spread in approximately all parts of the world. Its invasion is probably due to the contamination of its seeds in the imported grains from other countries of the world.  Objective:  During comprehensive sampling from Pakistan and Australia, it was observed that parthenium weed accessions exhibited several distinct morphological features...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parthenium hysterophorus; Variations; Genetic diversity; MEGA; Haplotypes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100302

Molecular characterization of Aedes aegypti (L.) (Diptera: Culicidae) of Easter Island based on analysis of the mitochondrial ND4 gene
Provedor de dados: 92
Autores: Núñez,Claudia Andrea; González,Christian Raúl; Obreque,Víctor; Riquelme,Brenda; Reyes,Carolina; Rojas,Mabel.
ABSTRACT Aedes aegypti mosquitoes are the main vector of viruses Dengue, Zika and Chikungunya. Shortly after the first report of the dengue vector Ae. aegypti in Easter Island (Rapa Nui) in late 2000, the first disease outbreak dengue occurred. Viral serotyping during the 2002 outbreak revealed a close relationship with Pacific DENV-1 genotype IV viruses, supporting the idea that the virus most likely originated in Tahiti. Mitochondrial NADH dehydrogenase subunit 4 (ND4) DNA sequences generated from 68 specimens of Ae. aegypti from Easter Island reporting a unique finding of a single maternal lineage of Ae. aegypti on Easter Island.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dengue; Haplotypes; Rapa Nui.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000200186

Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences
Provedor de dados: 49
Autores: Borba,Rafael Splendore de; Silva,Edson Lourenço da; Ponzetto,Josi Margarete; Pozzobon,Allan Pierre Bonetti; Centofante,Liano; Alves,Anderson Luis; Parise-Maltempi,Patrícia Pasquali.
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flood pulse; Genetic diversity; Gene flow; Haplotypes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000100004

Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region
Provedor de dados: 56
Autores: Piranda,D.N.; Abreu,R.B.V.; Freitas-Alves,D.R.; de Carvalho,M.A.; Vianna-Jorge,R.
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P&lt;0.01). Despite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COX-2; PTGS2; Haplotypes; Gene expression; MCF-7; HEK293FT.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200602

Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays
Provedor de dados: 56
Autores: Apalasamy,Y.D.; Ming,M.F.; Rampal,S.; Bulgiba,A.; Mohamed,Z.
The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FTO gene; SNP; Obesity; Linkage disequilibrium; Haplotypes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200003